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2020 Spring Meeting

Biomaterials and soft materials

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Bioinspired and biointegrated materials as new frontiers nanomaterials

This symposium is composed of SPECIAL SESSIONS contemporary hottest topics from a field a nature inspiration - mimetic single, supramolecular nanomaterials to micromimetic materials with mimetic supramolecular chemistry and which will be smart living building blocks, with LIFE Inspired processes, of smart materials for better Human Life.

Scope:

Newest science ideas and nanotechnologies for smart nano - materials, - integrated systems, - robotic devices fields which determine developing biomimetic cells and skin, bone tissue engineering, remodeling ones and adaptation to a regeneration of neural systems using created implantable bionic systems. These systems can include molecular systems, bioimmobilized Nanoparticles NPs as nanorobots in vivo applications and designed synthesized supramolecules which are templated by biomolecules (virus, marine plants, proteins, pigments) inorganic NPs for the quantum dots nanosystems, bioinspired composites –Biohybrids- materials ...

The design, engineering of these materials are aimed to obtain the properties which respond to external, biologically compatible stimuli (physical, chemical, biological) and to electronic, photonic, magnetic nanosystems. Next step is transferring from nano to macro materials for regenerative medicine of bones and teeth (stem cells regenerative orthopedic and dental medicine), for example, and engineering of multifunctional biointerfaces and biotemplating.

The symposium will bring together researchers from chemical, physical sciences and bio - science and – nanotechnology biomaterials for nanomedicine and engineering bio - electronic, - photonic, - magnetic nanosystems to discuss the latest advancements.

Proposed subjects for discussions at this event have actuality for Investigators - Collaborators of the EU COST Actions, as and the EU HORIZON 2020-2025 Projects. A special Young Scientist FORUM - the session for Advanced Researchers (Post-Graduate, PhD and Graduate students’ talks) will be held at the symposium’s second day.

Hot topics to be covered by the symposium:

  1. Designed synthesized single, supramolecular materials and their action in bio – mimetic material synthesis;
  2. Bioinspired synthesis of inorganic nanoparticles (NPs), hybrid systems with smart activity;
  3. Design and creation of the 2D and 3D nanocarbon’s smart supramolecular materials and their Biohybrids;
  4. Novel concepts in nano - characterization, bio-recognition of smart and specially bio-hybrid stimuli responsible nanomaterials (biosynthesized, bioimmobilized and biointegrated inorganic nanoparticles, carbon and bioimmobilized carbon supramolecules)with applications for clinical, food, feed and environmental monitoring;
  5. 2D-3D-4D molecular imprinting biological cell structures and biomimetics ones as scaffolds in tissue engineering with specially cell, tissue actuating, manipulation using constructed bionic systems;
  6. Electronic, photonic and magnetic smart functions of biosupramolecules (nucleic acids, virus, marine plants proteins, pigments) and mimetic analogs: adaptation to human systems functions for biomedical nano – systems, - robotic devices designing, for example, molecular robot - DNA motor “robotic” molecule into living cell; Special – Neuroelectronics;
  7. Biosensing characterization for medical (in vivo and in vitro diagnostics) and environmental biotechnologies (developed 3rd Generation Biosensors on biomolecules, carbon 2D materials, bioelectronic textiles, wears tissues, printed paper electronics, electronic skin);
  8. Biomimetic analogs functions in compare to bionic functions for natural systems (electronic skin, neuroengineering).
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08:50 The 10th edition Symposium Presentation - Professor Insung S. Choi (KAIST, Korea)    
 
Smart Nanomaterials and Nanosystems Strategy from Nature FORUM : Chairs - Dr. Emmanuel I.Stratakis (Institute of Electronic Structure and Lasers -FRT Hellas,Greece), Professors: Insung S. Choi (KAIST,Korea), Bert Mueller (University of Basel, Switzerland),Yutaka Wakayama (National Institute for Materials Science, Japan), Joerg K.N.Linder ( Universitat Paderborn, Germany), Roger Woerdenweber (Universitat zu Koeln, Germany)
09:00
Authors : Prof. Dr. Bert Muller
Affiliations : Director, Biomaterials Science Center (BMC), University of Basel, Gewerbestrasse 14 4123 Allschwil Switzerlan bert.mueller@unibas.ch

Resume : Biomaterials nanoscience and nanotechnology development determinate Nanomedicine success because Nanomedicine, also termed nanotechnology-enabled medicine [1], is the science and technology of diagnosing, treating, and preventing diseases and traumatic injuries, of relieving pain, and of preserving and improving health using molecular tools and molecular knowledge of the human body according to the European Science Foundation. To clearly distinguish nanomedicine from established treatment forms, it can alternatively be defined as characterizing hard and soft tissues on the nanometer scale and tailoring nanostructured man-made materials for improving human health. The understanding of tissue organization down to its nanometer-size components and the development of interrelated tools to prevent, diagnose, and treat diseases are essential steps in current clinically applied science. The societal need for cost-effective improvements of patient health thereby motivates the research activities in nanomedicine and their scientific approaches [2]. These efforts are converged on targeting applications in orthopedics and dentistry; oral and musculoskeletal biology are both largely driven by mechanical forces, and the prosthesis materials in both applications require matching the unique nanostructure and material properties of the host tissues [3].It makes sense to adopt the principals of Nature and take advantage of these. [1] B. Müller, Nanomedicine at a glance Book chapter 1 "in Nanoscience and Nanotechnology: Advances and Developments in Nano-sized Materials? (Ed. M.Van de Voorde) De Gruyter (2018) pp. 3-15 DOI:10.1117/12.2317867 [2] C. Netzer, P. Distel, U. Wolfram, H. Deyhle, G. F. Jost, S. Schären, J.Geurts Comparative analysis of bone structural parameters reveals subchondral cortical plate resorption and increased trabecular bone remodeling in human facet joint osteoarthritis International Journal of Molecular Sciences 19 (2018) 854 DOI:10.3390/ijms19030845 [3] C. Bikis, L. Degrugillier, P. Thalmann, G. Schulz, B. Müller, S. E.Hieber, D. F. Kalbermatten, S. Madduri Three-dimensional imaging and analysis of entire peripheral nerves after repair and reconstruction, Journal of Neuroscience Methods 295 (2018) 37-44 DOI:10.1016/j.jneumeth.2017.11.015

R.1.1
09:25
Authors : Thomas J. Webster
Affiliations : Chemical Engineering, Northeastern University, Boston, MA 02115

Resume : Nanotechnology was first defined in the 1959 Nobel lecture, "There is plenty of room at the bottom," presented by the Nobel Laureate, Prof. Richard Feynman. Ever since, numerous researchers have investigated the role of nanotechnology in many aspects of life and science. During recent decades, nanotechnology has captured wide attention across various areas such as energy, the environment, cosmetics, medicine and healthcare, drugs, information technology, and so forth. Particularly, the emergence of nanotechnology has set high expectations in medicine and biological science. In the near future, nanotechnology may be able to solve many key questions concerning biological systems that currently burden society. This is partially due to the fact that the sizes of functional elements in biology (such as amino acids, sugars, nucleotides, proteins, and DNA) are in the nanometer range and therefore promote the interaction between nanomaterials and biological systems. For example, by altering or manipulating the topographical features of conventional biomaterials that possess micron scale or larger surface features, one can create nanomaterials that promote interactions with native tissues on similar nanometer scales. The application of nanotechnology to medicine has led to the creation of a new field of research with an entirely new horizon of applications and possibilities. Nanomedicine allows scientists and researchers to study biological systems at the nanoscale range and to mimic or refine mechanisms to replicate natural tissues. In this regard, one may consider the complexities and difficulties that can arise when combining nanotechnology with human physiology; therefore, a fundamental understanding of both nanotechnology and their respective physiological counterparts is essential to develop beneficial nanotechnology. For instance, in the realm of utilizing biomaterials for regenerative medicine, promising biomaterials must simultaneously boost tissue regeneration while minimizing immune responses and preventing infection. This talk will cover some of the more significant advancements of nanomedicine over the past 14 since, since the founding of the open access journal, International of Nanomedicine.

R.1.2
09:40
Authors : Gonen Ashkenasy
Affiliations : Ben-Gurion University of the Negev, Beer Sheva, Israel

Resume : Synthetic and Materials Chemistry initiatives have enabled the translation of the macromolecular functions of biology into new frameworks. These explorations into alternative chemistries of life attempt to capture the versatile functionality and adaptability of biopolymers in orthogonal scaffolds. Information storage and transfer however, so beautifully represented in the Central Dogma of biology, require multiple components functioning synergistically. Consequently, studies in Systems Chemistry are now beginning to incorporate mutualistic biopolymer networks.[1] Our approach towards studying synergistic behavior started with amyloid-like assemblies as potential alternative genetic elements, inducing regio- and stereo-selective replication.[2] The successful integration of both chemical and physical processes through β-sheet assisted replication processes crucially informed the synergistic potential of these networks. Inspired by the cooperative synergies of nucleic acids and proteins in biology, we have recently explored synthetic nucleic-acid-peptide chimeras, with the aim to extend their assembly, binding, and replication capacity.[3] 1. Bai, Chotera, Taran, Liang, Ashkenasy, Lynn “Achieving Biopolymer Synergy in Systems Chemistry" Chem. Soc. Rev. 2018, 47, 544. 2. Nanda, Rubinov, Ivnitski, Mukherjee, Shtelman, Motro, Miller, Wagner, Luria, Ashkenasy. Nature Commun. 2017, 8, 434. 3. Chotera, Sadihov, Cohen‐Luria, Monnard, Ashkenasy. Chem. Eur. J. 2018, 24, 10128.

R.1.3
09:55
Authors : Emilie Moulin, Nicolas Giuseppone
Affiliations : Institut Charles Sadron ? UPR 22, University of Strasbourg, SAMS Research Group, 23 Rue du Loess, BP84047, 67034 Strasbourg Cedex 2, France

Resume : Switchable functional molecules capable of producing mechanical work constitute an active focus in nanotechnologies as they can be a source of components for molecular-based devices and materials. Many examples of such nano-machines operating at the molecular level and including molecular actuators, molecular electronic components, and molecular nano-valves have been developed. However, one of the most fundamental and challenging objectives associated to nano-machines rests on their coupling (in space and time) in order to transfer controlled motions from the molecular arena to the macroscopic scale, similarly to living systems such as muscular tissues. Here, using a combination of organic synthesis and characterization by scattering and microscopy techniques, we will show that nanomachines can be used to build responsive contractile macroscopic materials such as gels or films which can behave as artificial muscles, with possible applications in robotic devices. Two well-defined systems will be presented: i) one based on single contractile nano-switches which can be polymerized to form single-chain supramolecular polymers and further produce micro- and macroscale motions by integration of their nanoscopic movement; ii) a second one based on the connection of rotary motors with the possibility to make them functioning far from equilibrium (that is under light energy supply) and in connection within a cross-linked polymer network.

R.1.4
10:10
Authors : Emmanuel Stratakis1,2
Affiliations : 1Foundation for Research and Technology - Hellas (F.O.R.T.H.), Institute of Electronic Structure and Laser (I.E.S.L.), Heraklion, Crete, Greece, 2 Physics Department, University of Crete, Heraklion, Crete, Greece

Resume : The surface topography of biomaterials can have an important impact on the cellular adhesion, growth and proliferation. Apart from the overall roughness, the detailed morphological features at all length scales, significantly affect the cell-biomaterial interactions in a plethora of applications including structural implants, tissue engineering scaffolds and biosensors. We present simple, one-step direct femtosecond (fs) laser patterning techniques to fabricate various types of micro/nano structured biomaterials platforms. Variation of the laser fluence, alters the surface morphology of solid materials, leading to a rippled-type, at lower laser fluences, or a conical spiked-type morphology, as the laser fluence increases. Hierarchically-structured cell culture platforms incorporating gold nanoparticles functionalized with specific bio-functional moieties have been additionally developed. Cells with nerve cell phenotype were cultured on the substrates. More specifically, PC12 cells cultured on the developed substrates and treated with nerve growth factor showed a differentiation response that was highly dependent on the surface topography. While, experiments with DRG/SCG nerve cells showed a differential orientation of the cells, depending of the underlying geometry of the laser engineered surface structures (Figure 1). Depending on the laser processing conditions, distinct SW10 cell-philic or cell-repellent patterned areas can be attained with a desired motif, enabling spatial patterning of cells in a controllable manner. Furthermore, we report on the fs laser microfabrication of porous collagen scaffolds, providing implants that via precise micron-sized features held the promise to control neuron cell phenotypes. Finally, the fs laser fabrication of a novel microfluidic platform for the study of the combined effect of fluid shear forces and culture substrate morphology on neuron cell proliferation and directionality will be demonstrated. Our work provides a versatile laser-based biofabrication approach to tune neuron cell responses by proper selection of the surface free energy of the substrate and may be promising for the design of cell culture biomaterial platforms for neural tissue engineering applications.

R.1.5
10:30 11:00 Coffee Break, General Photo    
11:00
Authors : Insung S. Choi
Affiliations : Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Korea

Resume : Single-cell nanoencapsulation is defined as the cytocompatible chemical strategy to physically confine individual living cells with ultrathin (preferably, < 100 nm), tough shells in the three-dimensional space. The cellular hybrid structures generated by single-cell nanoencapsulation have been called various names, such as cell-in-shell structures, artificial spores, micrometric Iron Men, cyborg cells, or SupraCells. Since concept development in 2013, the field has rapidly grown and recently entered the second stage of development, where the artificial shells are actively and intimately involved in the cellular metabolism and activities, not just protecting the cells from harmful aggressors. The past, present, and future of single-cell nanoencapsulation are discussed concisely.

R.1.6
11:20
Authors : Karsten Haupt; Paulina Medina Rangel; Jingjing Xu; Bernadette Tse Sum Bui
Affiliations : Sorbonne Universities, Université de Technologie de Compiègne, CNRS Institute for Enzyme and Cell Engineering, Compiègne, France

Resume : Molecularly imprinted polymers (MIPs) are synthetic antibody mimics that specifically recognize molecular targets.1,2 They are highly cross-linked polymers synthesized in the presence of the target molecule ore an epitope thereof, acting as a molecular template. This templating induces three-dimensional binding sites in the polymer that are complementary to the template in size, shape and chemical functionality. The synthetic antibody can recognize and bind its target with an affinity and selectivity similar to a biological antibody.1 Herein, we demonstrate the potential of MIPs for antibody therapy on the example of two cell surface biomarker targets, glycans and a cell-cell adhesion protein.3 We also present the potential use of MIPs for the inhibition of cell-virus interactions.4 Finally, their applications in diagnostics and bioanalysis will be demonstrated.3,5

R.1.7
11:40
Authors : Marleine Tamer (a,b), Maria Bassil (b), Mario El Tahchi (b), Sebastien Balme (a), Philippe Miele (a), and Mikhael Bechelany (a)
Affiliations : a. European Institute of Membranes, ENSCM, CNRS, University of Montpellier, France.; b. Laboratory of Biomaterials and Intelligent Materials, Department of Physics, Lebanese University - Faculty of Science II, Jdeidet, Lebanon.

Resume : Organs-on-a-chip technology has shown strong promise in organ replication, combining bioengineering and microfluidic technologies to closely mimic the natural organ [1,2]. Kidney-on-a-chip has emerged as a novel strategy for the improvement of filtration function in the case of kidney disease [3]. Inspired by the natural organ and the dialysis system, we designed a new bioartificial kidney device composed of several filtration units. Each unit encapsulates a Polyacrylamide hydrogel matrix, considered as functional unit of the device and acts as an exchange membrane. Several hydrogel matrices are prepared and their physicochemical properties are tailored as desired in order to test their effects on the filtration ability and efficiency of the “filtration unit”. Results show that the speed of solute diffusion across the matrix is controlled by varying the pores number, while the size selectivity and the molecular weight cutoff of the matrix depend on the pore size. Bovine serum albumin 66 kDa and Fluorescein isothiocyanate–dextran (MW: 40, 70, 150 kDa) were used to test the molecular weight cutoff of PAAM membrane as they mimic the blood proteins. The results show a restriction of the passage of proteins having a molecular weight above 66 kDa across the membrane. The ion selectivity of the membrane was tested to verify the stability of a normal body fluid composition. The future bioartificial kidney would be an assembly of several filtration units working in parallel each having a specific function and imitating a specific renal activity. 1. Zheng, F. et al. Organ-on-a-Chip Systems: Microengineering to Biomimic Living Systems. Small 12, 2253–2282 (2016). 2. Ashammakhi, N., Wesseling-Perry, K., Hasan, A., Elkhammas, E. & Zhang, Y. S. Kidney-on-a-chip: untapped opportunities. Kidney Int. 94, 1073–1086 (2018). 3. Wilmer, M. J. et al. Kidney-on-a-Chip Technology for Drug-Induced Nephrotoxicity Screening. Trends Biotechnol. 34, 156–170 (2016).

R.1.8
12:00
Authors : Abdurrahim Can Egil, Egemen Acar, Gozde Ozaydin Ince
Affiliations : 1 Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey, 2 Sabanci University Nanotechnology Research and Application Center (SUNUM), Sabanci University, Istanbul, Turkey

Resume : In recent years, there has been considerable interest on the development of functional polymeric nanoparticles for controlled drug delivery applications due to their biocompatible nature and ability to tune their response to changes in the environment. Nanoparticles synthesized from stimuli responsive materials enable the triggered release of the drugs at a controlled rate at the target. Chitosan (CS), a widely used polymer in drug delivery, exhibits pH response at acidic environment. Poly-N-Vinyl-Caprolactam (PNVCL), on the other hand, is a thermoresponsive polymer which undergoes phase transition at a lower critical solution temperature (LCST) of approximately 32 °C. The LCST value can be tuned by copolymerization of the n-vinyl-caprolactam with other hydrophilic monomers such as acrylic acid (AA). In this study, pH and thermo responsive positively charged core shell nanoparticles were synthesized with p(NVCL-co-AA) polymer as the core and chitosan as the shell. Potassium persulfate was used as the initiator for surfactant free copolymerization of NVCL with AA in the presence of chitosan, and Temozolamide was chosen as the model drug. Scanning electron microscopy (SEM) was used to image the morphology of the nanoparticles and Fourier-transform infrared spectroscopy (FTIR) was utilized for the chemical characterization. The average size, zeta potential and polydispersity index (PDI) of the synthesized nanoparticles were measured using dynamic light scattering. Ultraviolet-visible spectrophotometer was used in order to obtain encapsulation efficiency, loading capacity and release profile of loaded nanoparticles. The synthesized positively charged nanoparticles showed the ability to collapse at high temperatures due to the thermoresponsive core and to swell at acidic environment because of the pH responsive chitosan shell. Dual response of the nanoparticles enabled fast and triggered release of the drug at the acidic conditions of the tumor extracellular site and endosome and lysosome environment. The particles retained the drugs at a higher pH value of 7.4, preventing the early release of the drugs before reaching the target. Due to the ability to tune the response of the drug-loaded nanoparticles to the changes in the environment, these nanoparticles can potentially be used for the delivery of various drugs under different physiological conditions.

R.1.9
12:15
Authors : Ahmed Al-Kattan
Affiliations : Aix-Marseille University, LP3 laboratory, Marseille, France

Resume : Driven by surface cleanness, unique physical and chemical properties, bare (ligand-free) laser- synthesized nanoparticles (NPs) are now in the focus of intensive researches in a wide range of applications including environment, catalysis and biomedicine. Indeed, based on the interaction of ultrafast laser beam in liquid ambiance (e.g., aqueous solution) with a solid target material, this process leads naturally to the formation of spherical NPs with modulate physicochemical properties including diameter and size dispersion, surface chemistry and oxidation rate [1]. Recently, in our group we have thus demonstrated the possibility to elaborate ultraclean and very stable colloidal suspension of AuNPs and SiNPs with unique physicochemical properties for biomedical applications [1-3]. For instance, we have shown the possibility to modulate the dissolution behavior of SiNPs by varying the amount of dissolved oxygen in water [2]. We have also evidenced the complete safety properties of AuNPs and SiNPs using in vivo nude mice animal model [4,5]. As one of the major result, we shown that the SiNPs can be also exploited as significant sensitizers of radiofrequency (RF)-induced hyperthermia on Lewis lung carcinoma with efficient tumor inhibition at relative low concentration [6]. Very recently, we have also elaborated promising alternative plasmonic tools based on TiN NPs for potential photothermal therapy modalities [7]. Beside conventional additives mainly made-it by chemical way, we have also started to explore such bare laser synthesized NPs as novel functional additives for tissue engineering applications [8]. [1] A. Al-Kattan et al, IJMS, 2018, 19(6), 1563). [2] A. Al-Kattan et al, J. Mater. Chem. B, 2016, 4, 78523 [3] F. Correard et al, Int. J. Nanomed., 2014, 9, 54152 [4] T. Baati et al, Sci. Rep., 2016, 6, 25400 [5] A.L. Bailly et al, Sci. Rep., 2019, 9, 1258 [6] K.P. Tamarov et al, Sci. Rep., 2014, 4, 7034 [7] A. Popov et al, Sci Rep, 2019, 9, 1194 [8] A. Al-Kattan et al, RSC Adv., 2017, 7, 31759

R.1.10
12:30
Authors : Zied Ferjaoui, Raphaël Schneider, Lina Bezdetnaya, Eric Gaffet, Halima Alem
Affiliations : Zied Ferjaoui, Eric Gaffet, Halima Alem: Institut Jean Lamour (IJL), UMR CNRS 7198, Université de Lorraine, Department N2EV, allée André Guinier - Campus Artem 54000 Nancy, France Lina Bezdetnaya: Institut Cancérologie de Lorraine, 6 Avenue de Bourgogne CS 30519 54519 Vand?uvre-lès-Nancy, France Raphaël Schneider :Laboratoire Réactions et Génie des Procédés (LRGP), UMR CNRS 7274, Université de Lorraine, 1 rue Grandville 54001 Nancy, France

Resume : Grafting functional biocompatible thermo-responsive copolymers at the surface of superparamagnetic nanoparticles (NPs) is a promising route to develop new targeting nano-carriers for cancer therapy. In this work, we report on the development and the characterization of superparamagnetic and thermo-responsive core/shell NPs which were used as a drug delivery based system. These NPs were obtained by the growth via controlled living polymerization of co-polymers based on 2-(2-methoxy) ethyl methacrylate (MEO2MA) and oligo (ethylene glycol) methacrylate (OEGMA) moieties leading to P(MEO2MAx-OEGMA100-X) (the ratio of the both monomers were varied to tune the LCST)1?5. We demonstrate that the NPs are able to load and release a model drug, i. e. doxorubicine (DOX) with an associated DOX loading capacity of 93%, markedly higher than the current nanocarrier performances. Those new systems exhibit also hyperthermia properties. The cytotoxicity results showed that the core/shell NPs exhibit limited cytotoxicity up to concentration of 12 mg.mL-1 towards SKOV3 human ovarian cancer lines. Upon DOX release from DOX-loaded NPs, the NPs are much more cytotoxic towards those cells than free DOX. Moreover, when folic acid (FA) was anchored at the periphery of these NPs3, an enhancement of the selective binding and of the delivery of the drug to the targeted cells (SKOV 3) was demonstrated. This study offers a promising route to improve the current available drug nanocarrier for cancer therapy combining in one nano-object hyperthermia and chemotherapy.

R.1.11
12:45
Authors : Valerio Voliani
Affiliations : Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, P.zza San Silvestro, 12 - 56127, Pisa (PI), Italy.

Resume : Plasmonic noble metal nanoparticles (NPs) have attracted increasing interest for their potentiality to revolutionize diagnosis and treatment of many diseases, especially neoplasms. Nonetheless, there are still no clinically approved NPs for cancer therapy/diagnostic and very few candidates are in clinical trials. The clinical translation of NPs is mainly prevented by their persistence in organism after the medical action. Such persistence increases the likelihood of toxicity and the interference with common medical diagnoses. Size-reduction to ultrasmall nanoparticles (USNPs) is a suitable approach to promote metal excretion by the renal pathway, however altering most of the behaviors of NPs. A groundbreaking advance to jointly combine the appealing features of NPs with metal excretion relies on the ultrasmall-in-nano approach. Within this approach, we have designed inorganic all-in-one biodegradable nano-platforms comprising plasmonic USNPs: the nature-inspired passion fruit-like nano-architectures (NAs). The versatility of NAs production will be presented, together with the significant metal-excretion trend from murine models and preliminary applications, in particular for photothermal treatments. Furthermore, the last achievements from this novel approach will be discussed and the next exciting perspectives provided. Such nano-architectures might bring again noble metal nanomaterials to the forefront of cancer theranostics, in order to treat carcinomas in a less invasive and more efficient manner. The research leading to these results has received funding from AIRC under MFAG 2017 ? ID 19852 project ? P.I. Voliani Valerio.

R.1.12
13:00 14:00 Lunch Break    
14:00
Authors : Silvia Gross
Affiliations : Dipartimento di Scienze Chimiche Università degli Studi di Padova via Francesco Marzolo, 1 35131- Padova Italy

Resume : Control on shape, morphology, size and crystallinity of inorganic nanomaterials are major requirements not only in the fields of catalysis, but also for bio-oriented and environmental applications. In this context, the paradigms of green and sustainable chemistry are currently raising a sharply growing interest in all fields of inorganic materials chemistry [1]. In this framework, resorting to sustainable, green and easy scalable wet-chemistry, typically aqueous-based, synthesis routes is a convenient approach to produce nanostructures for different functional applications. In this framework, we have explored and optimised different low temperature (T< 150°C) and sustainable wet chemistry and colloidal routes [1, 2] and developed an efficient and versatile synthetic toolbox, encompassing (i) hydrothermal routes [3], (ii) very low (0°C) temperature precipitation, (iii) continuous flow synthesis, (iv) seeded-growth) and combinations thereof, to prepare different inorganic functional nanomaterials ranging from metal and metal alloys nanoparticles, pure and doped metal oxides for optical bioimaging and possible oxidation catalysis reactions, doped copper and zinc sulphide for optical bioimaging and bio-oriented applications. Cytoxicity studies were also performed for in vitro applications. Common factors of all these approaches are the low temperature of processing, the easy procedure, the reproducibility, the possibility to up-scale the optimised route. [1] S. Diodati, P. Dolcet, M. Casarin, S. Gross; Chem. Rev. 2015, 115, 11449?11502

R.1.13
14:20
Authors : Jörg K. N. Lindner
Affiliations : Department of Physics, Paderborn University, Germany; Institute for Lightweight Design with Hybrid Systems ILH, Paderborn; Centre for Optoelectronics and Photonics Paderborn CeOPP, Paderborn.

Resume : More than twenty years ago, the mutual investigation of objects on the nanoscale was predicted to lead to the convergence of sciences like biology, chemistry and physics with fruitful applications in various technologies. A blazing example of such objects are regular nanopatterns on solid surfaces which can be easily created on large areas by exploiting the self-assembly of block-copolymers (BCPs). Various patterns with sub-ten to few-ten nanometer feature size can be fabricated, depending on the application in mind: While lamellar nanopatterns are candidates for next generation ultra-large scale integrated electronics, ordered arrays of cylindrical dots or nanocylinders hold promise of enabling the controlled placement of quantum dots, catalysts, and biological entities such as drugs and proteins. Macroscopic surface properties such as optical reflectivities and wettabilities can be precisely tuned by controlling the nanopatterns, allowing to exploit patterned surfaces e.g. in the biomed area. At the same time, all these applications require a detailed knowledge of the morphological and chemical characteristics of nanopatterns. This can be achieved using advanced (scanning) transmission electron microscopy ((S)TEM) in combination with other techniques. The presentation will shed light on recent progress in the characterization of BCP thin films ordered on the nanoscale and their applications in the fields mentioned above.

R.1.14
14:35
Authors : Alessandro Iannaci, Adam Myles, James A. Behan, Thomas Flinois, Eoin M. Scanlan, Frédéric Barrière and Paula E. Colavita
Affiliations : School of Chemistry, CRANN and AMBER Research Centres, Trinity College Dublin, College Green, Dublin 2, Ireland; Univ Rennes, CNRS, Institut des Sciences Chimiques de Rennes - UMR 6226, F-35000 Rennes, France

Resume : Surface-bound glycans hold potential as versatile tools for tailoring the interactions of biomolecules, cells and organisms with solid surfaces via regulation of either specific or non-specific interactions. We have recently focused on the development of precursors and surface modification protocols for the immobilization of carbohydrates based on the chemistry of aryldiazonium cations, with the objective of offering a scalable one-step route to their covalent immobilization onto carbon, metals and polymers. In this work we will discuss applications of functional saccharide thin layers in which we leverage both the ability to regulate non-specific adsorption and the ability to promote affinity binding via bio-recognition of selected glycans. First, we show that, in the absence of specific recognition, we can achieve fouling mitigation in complex biomass matrices. This is of vital importance for instance in electroanalysis and we demonstrate applications of such layers to voltammetric detection at carbon electrodes. Second, we show that use of glycans that are specifically recognised by lectins can be leveraged to promote bacterial recruitment and colonization of surfaces. We demonstrate applications of this approach to electrocatalysis at bioanodes in bioelectrochemical systems such as microbial fuel cells. A selected glycan layer was found to accelerate electrode colonization and fuel cell start-up times. The scalability of aryldiazonium reactions further supports the possibility of extending this strategy to devices with larger total power outputs based on processing of wastewater or other biomass sidestreams.

R.1.15
14:50
Authors : G. I. Márk1, K. Kertész1, G. Piszter1, Zs. Bálint2, and L. P. Biró1
Affiliations : 1 Institute of Technical Physics and Materials Science, (MFA), Centre for Energy Research, Hungarian Academy of Sciences, Budapest, Hungary, http://www.nanotechnology.hu/ 2 Hungarian Natural History Museum, Baross Utca 13, H-1088 Budapest, Hungary

Resume : Nature often applies photonic nanostructures in butterfly wing scales. These nanostructures play an important biological role in sexual communication, because they can produce various optical effects, e.g. iridescent or non-iridescent colours. The colour generating scales are flat sacs of dried cuticle, containing a quasi ordered arrangement or air voids. The optical effects are generated by constructive and destructive interference of light reflected and scattered on the boundary surfaces of these voids. The thickness of the scale is often as small as 1-2 m, still it can reflect conspicuous colours. We examine in detail the importance of multiple light scattering in butterfly wing optical spectra. While the spectral position and width of the main peak can often be predicted by first order scattering calculations, second- and higher order scattering contributes to the details of the spectrum, mainly on its small wavelength (UV) region. References 1. Biró, L. P. and Vigneron, J. P. “Photonic nanoarchitectures in butterflies and beetles: valuable sources for bioinspiration”, Laser Photon. Rev. Vol. 5, 27–51, 2011. 2. Bálint, Z., Kertész, K., Piszter, G., Vértesy, Z., and Biró, L. P. “The well-tuned blues: the role of structural colours as optical signals in the species recognition of a local butterfly fauna (Lepidoptera: Lycaenidae: Polyommatinae)”, J. R. Soc. Interface Vol 9, 1745–56, 2012. 3. Wilts, B. D., Leertouwer, H. L., and Stavenga, D. G. “Imaging scatterometry and microspectrophotometry of Lycaenid butterfly wing scales with perforated multilayers”, J. R. Soc. Interface 6 Suppl 2, S185-92 (2009). 4. Márk, G. I., Vértesy, Z., Kertész, K., Bálint, Zs., and Biró, L. P. “Order-disorder effects in structure and color relation of photonic-crystal-type nanostructures in butterfly wing scales”, Phys.Rev.E Vol 80, 051903-1, 2009.

R.1.16
15:05
Authors : Masaya Nogi, Kasuga Takaaki, Chenyang Li, Yunxia Wang
Affiliations : The Institute of Scientific and Industrial Research, Osaka University

Resume : Cellulose nanofiber with 3-15 nm wide is the main components of plat cell wall. Nanopaper consisting of only cellulose nanofiber is highly transparent, high smooth surface and foldable. Therefore, it is one of the best candidate for the flexible and disposable electronics. This presentation will introduce the recent our three achievements. First one is the transparent nanopaper fabrication procedures, which is rapid drying condition of cellulose nanofiber dispersion. Second one is high performance flexible cellulose nanofiber capacitor. The last one is the nanopaper sensor device that combines humidity sensing, wireless information transmission, and degradability has been fabricated using cellulose nanofibers.

R.1.17
15:20
Authors : Arzum Erdem
Affiliations : Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova, 35100 Izmir, TURKEY email: arzum.erdem@ege.edu.tr and arzume@hotmail.com Web site: www.arzumerdem.com/indexen.html

Resume : Electrochemical nucleic acid biosensors have an intrinsic specificity towards to biorecognition process with high sensitivity of physical transducers to evaluate the nucleic acid hybridization and also, the interaction occured between DNA and a target analyte (i.e, drugs, proteins, toxins etc). Single-use electrochemical biosensors could provide easy and rapid analysis resulting in a short time with high sensitivity and reproducubility. An overview to single-use electrochemical nucleic acid biosensors has been discussed herein with their further applications on biomedical diagnostics. Acknowledgements. A.E acknowledges to the financial support from Turkish Science and Technology Research Council (TÜB?TAK) (Project No: 114Z400) as the Project Investigator, and she also would like to express her gratitude to the Turkish Academy of Sciences (TÜBA) as the Principal member for its partial support.

R.1.18
15:40 16:00 Coffee Break, General Photo    
16:00
Authors : Yutaka Wakayama
Affiliations : International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS)

Resume : A main purpose of this study is to develop a new ultra-highly sensitive sensor in particular for the detection of Cs ions in natural water. The long-time effect of radioactivity of Cs is known to be responsible for dramatic health condition of humans, as well as aquatic plants and animals. Therefore, the detection and monitoring of Cs ions in natural water are necessary. Our sensor is based on an organic field-effect transistor (OFET) structure, which is composed of three main components: (1) organic semiconducting channel with high stability in working with natural water, (2) monolayer lipid membrane working as ultra-thin dielectric layer to allow low operating voltage and high sensitivity, and (3) novel calixarene-crown ether probe for high selectivity to Cs, which is grafted with the lipid layer. In comparison with other conventional methods like ICP-MS, our sensor has many advantages: portability for on-site monitoring, high sensitivity on the femtomolar (pg/l) level of Cs ion and a high selectivity against competing ions, such as K, Na and Cl.

R.1.19
16:15
Authors : L.Possanzini(1), I. Gualandi(2), M. Tessarolo(1), F. Mariani(2), F.Decataldo(1), E. Scavetta(2) and B. Fraboni(1)
Affiliations : (1) Dipartimento di Fisica e Astronomia, Università di Bologna, Italy (2)Dipartimento di Chimica Industriale, Università di Bologna, Italy

Resume : The development of portable and wearable sensors is of high importance in several ?elds, such as point-of-care medical applications and environmental monitoring. To this end, Organic Electrochemical Transistors (OECTs) offer consistent advantages such as easy and cheap readout electronics, low supply voltage (usually < 1 V), low power operation (< 100 ?W), bio-compatibility, ease of integration. Moreover, the transistor configuration provides intrinsic amplification of the output signal and gives design freedom in terms of device geometries and substrates (flat/flexible). Here we report a new biosensing platform inspired by the organic electrochemical transistor (OECT), based on a composite material of PEDOT:PSS (poly(3,4-ethylenedioxythiophene) poly(styrene sulfonate)) and Ag/AgnX nanoparticles, where X refers to the ion to be detected in the fluid of interest (e.g. Cl-, Br-, I- and S2-). The Ag/AgX NPs act as a gate electrode embedded into the conductive polymer channel, thus combining an intrinsically amplified response with a simple two terminal electrical connection. Electrostatic Force Microscopy and Electrochemical Impedance Spectroscopy analyses demonstrate the electronic coupling between the electrochemically active NPs and the semiconducting polymer, which allows to explain the sensor ampli?ed transduction. The analytical signal is the current that ?ows in the composite polymer and its variation is directly proportional to the logarithm of Cl- concentration in the range 10?4 to 1 M. The simple, two terminal configuration of the here proposed biosensors has relevant positive implications on the read-out electronics, on the adaptability to unconventional geometries and on the response time, faster than for a conventional OECT endowed with a standard Ag/AgCl gate electrode [1]. Moreover, its ability to operate by sampling only a few microliters of fluid is ideal for wearable, non-invasive bio-fluid sampling. The analysis of concentrations of ions in biofluids, such as sweat, is crucial for several health conditions. In particular, the evaluation of Chloride concentration in sweat for infants and children is a method for diagnosing Cystic Fibrosis. Moreover, the presence of different ion concentrations in sweat can be directly related to dehydration and its real-time monitoring while training can help athletes control and improve their performance. The main bottlenecks for developing such non-invasive biosensors are the relatively large amount of biofluid needed for such analyses and the sensitivity and portability of the sensing system, issues that are fully overcome by the here proposed biosensors. We demonstrate their operation and performance in artificial sweat and we validate the implementation of our biosensors in a fully textile electronic device, fabricated directly onto a cotton yarn for real-time sweat monitoring. [1] I. Gualandi, M. Tessarolo, F. Mariani, T. Cramer, D. Tonelli, E. Scavetta and B. Fraboni, Sensors & Actuators: B. 273, 834 (2018)

R.1.20
16:30
Authors : R. Wördenweber, A. Markov, N. Wolf, Xiaobo Yuan, M. Glass, D. Mayer, V. Maybeck, A. Offenhäusser
Affiliations : Institute for Complex Systems (ICS-8), Forschungszentrum Jülich, 52425 Jülich, Germany; Institute for Bionic Technology and Engineering, First Moscow State Medical University, 119991, Moscow, Russian Federation

Resume : The interface between electrogenetic cells and inorganic surfaces represents one of the key elements for bioelectronic applications ranging from cell cultures and bioelectronics devices to medical implants. Here we describe a way to tailor the biocompatibility of substrates to guide cell growth and at the same time improve cell-chip communication. By using combinations of various oxides coated with different silanes, the neuron growth rate is significantly affected, patterned oxide layers equipped with silanes lead to guided cell growth, and the extracellular detected action potentials could be enhanced up to 5mV (neurons) and 10mV (HL-1) with a SNR of 50. Lithographically patterned surfaces of different oxides (e.g. SiO2, Al2O3, TiO2, Ta2O5) are generated and coated via gas-phase MLD with silane SAMs (e.g. APTES and GLYMO). By varying the ratio of the molecules in the SAM, we are able to tune the surface properties of the substrate (hydrophobicity, surface potential, etc.), the density and live/dead ratio of neurons. Using a combination of different oxides we are able to transfer the oxides' pattern via the SAM coating to the cell growth. Especially the combination of SiO2 and Ta2O5 leads to a large contrast of the cell density (20x). We believe that this technology could represent an ideal tool for engineering robust and reusable surfaces for bioelectronics purposes ranging from improvements in cell adhesion and cell proliferation to bioelectronics sensors or devices.

R.1.21
16:45
Authors : Bo ZHU
Affiliations : School of Materials Science and Engineering, Shanghai University, 99 Shangda Road, BaoShan, Shanghai, 200444, China

Resume : Bioelectronic implants, electrically coupled with nerve tissues, can help to resolve the sensory, motor, and cognitive deficits of the treated patients. These devices can serve as neural prostheses, brain-machine interfaces, nerve regeneration scaffolds, and bio-integrated devices for biological signal detection and recording. The long-term electrocoupling of the devices with nerve tissues are being challenged, however, by the immune system?s severe reaction to foreign bodies and the emigration of neuron cells. An ideal bioelectronic implant should combine flexibility, protein-resistance and cell-targeted electro-coupling to ensure the required biocompatibility and efficient electrical trade-offs for interfacing with cells/tissues. Being driven by this aim, we have adopted a biomimicking molecule design for both the polymer of electrodes and that of electric insulating packages. During the past several years, we synthesized a series of bio-mimicking conducting PEDOTs with either static, dynamic or 3D cell interaction of high selectivity and low impedance. All these conductive polymers have demonstrated an intimate, stable and efficient electrical electro-coupling with targeted cells by integrating nonspecific-binding resistance, specific interaction and low-impedance. As the insulating surface is the major part of the device surface, we also synthesized a protein resistant parylene polymer for further ensuring the biocompatibility of electronic implants. Most recently, we utilized the cell-selective EDOT polymer and the cell-resistant parylene polymer to construct a fully bio-mimicking OECT array device, which presents a spatially resolved and selective electro-coupling to targeted cells.

R.1.22
17:05
Authors : Yaopeng Zhang, Ao Zhuang, Bo Zhu
Affiliations : Donghua University, China

Resume : Conductive transparent film based on biomaterial substrate as electrode material is advancing towards various applications. Poly(3,4-ethylenedioxythiophene (PEDOT) and its derivatives have been well developed in this field as an excellent conductor. In this paper, hydroxymethyl-3,4-ethylenedioxythiophene (EDOT-OH) has been polymerized and deposited on the surface of regenerated silk fibroin (RSF) film in a aqueous system. Sodium dodecyl sulfate (SDS) was adopted as surfactant to form micelle which is beneficial to the polymer structure. To overcome the challenge of conflict between transparency and conductivity of PEDOT-OH coating, a composite oxidants recipe of iron chloride and ammonium persulfate (APS) was adopted. Through an electrostatic interaction of opposite doping ions, a well-organized conductive coating in nanoscale was constructed. As a result, a transparent conductive RSF/PEDOT-OH film was produced. With surface roughness and coating thickness in nanoscale, the square resistance Rs could reach 5.12×104? corresponding to the conductivity of 8.9× 10-2 S/cm, and the maximum transmittance of the film in visible region could reach above 73% at wet state at the same time. In addition, superior electrochemical stability and fastness of the film was demonstrated. High transparency of the film allowed a real-time observation of alive cells. The PEDOT-OH layer endowed an improved environment for absorption, proliferation and differentiation of PC12 cells than RSF surface, and a feasibility of electrical stimulation for cells.

R.1.23
17:25
Authors : Zhi Geng, Bo Zhu*
Affiliations : Zhi Geng, Lecturer, PHD, College of Materials Science and Engineering, Shanghai University, 99 Shangda Road, BaoShan, Shanghai, 200444, China, e-mail: gengzhi@shu.edu.cn; Bo ZHU, Professor, PHD, College of Materials Science and Engineering, Shanghai University, 99 Shangda Road, BaoShan, Shanghai, 200444, China, e-mail: bozhu@shu.edu.cn

Resume : Poly(3,4-ethylenedioxythiophene) (PEDOT) has been considered as next generation mateirlas in bioelectronics due to its excellent electrical conductivity, electrochemical stability and biocompability1-6. Introducing bio-functional groups would endow useful properties including strong cell-material interactions with good specificity, high resistance to the binding of nonspecific enzyme/cells, etc7,8. Nano assembling of bio-functionalized PEDOTs would further enhance the specific surface area for charge transporting and meet the requirement of communicating between micro sized bioelectronic devices and nerve cells with high signal-to-noise ratios and spatial resolutions. Therefore, designning simple but controllable nano-assembling methods for multiple kinds of bio-functionalized PEDOT materials was important in the field of bioelectronics. Templating method9,10 and fabricating nanocomposites11 had been reported to nano assemble the functionalized PEDOTS recently. Compared to templating method and nanocomposites, template-free assembling showed the beneficial of easy fabrication in large scale and integrated preservation of the conducting properties of PEDOTs and the achieved nanostructures, which would make it possible for fabricating mirco/nano electronic devices in wafer-scale. However, disadvantages were commonly existed, in which the surface morphology parameters could not be tuned precisely with template-free assembling. Starting from the assembling mechanism, we herein proposed a novel type of template-free method for fabricating tubular biofunctionalized PEDOTs with high controllability in morphology parameters, including diameter, length and density. Based on glancing incident wide angle XRD measurements, we found that the two phase systems in the electrochemical solution would lead to the formation of these tubular PEDOTs. Simulation tests were also carried out to verify the beneficial of the tubular functionalized PEDOTs in bioelectronic devices. The proposed method could be fuuly used in assembling various kinds of bio-functionalized PEDOT materials with controllable 3D nanomorphologies based on the requirements of bioelectronic devices. Reference: 1. Khodagholy, D.; Rivnay, J.; Sessolo, M.; Gurfinkel, M.; Leleux, P.; Jimison, L. H.; Stavrinidou, E.; Herve, T.; Sanaur, S.; Owens, R. M.; Malliaras, G. G. Nature Communications 2013, 4, 2133. 2. Rivnay, J.; Inal, S.; Collins, B. A.; Sessolo, M.; Stavrinidou, E.; Strakosas, X.; Tassone, C.; Delongchamp, D. M.; Malliaras, G. G. Nature Communications 2016, 7, 11287. 3. Williamson, A.; Rivnay, J.; Kergoat, L.; Jonsson, A.; Inal, S.; Uguz, I.; Ferro, M.; Ivanov, A.; Sjostrom, T. A.; Simon, D. T.; Berggren, M.; Malliaras, G. G.; Bernard, C. Advanced Materials 2015, 27, 3138. 4. Williamson, A.; Ferro, M.; Leleux, P.; Ismailova, E.; Kaszas, A.; Doublet, T.; Quilichini, P.; Rivnay, J.; Rozsa, B.; Katona, G.; Bernard, C.; Malliaras, G. G. Advanced Materials 2015. 5. Sotzing, G. A.; Briglin, S. M.; Grubbs, R. H.; Lewis, N. S. Analytical Chemistry 2000, 72, 3181. 6. Jalili, R.; Razal, J. M.; Innis, P. C.; Wallace, G. G. Advanced Functional Materials 2011, 21, 3363. 7. Sekine, J.; Luo, S. C.; Wang, S.; Zhu, B.; Tseng, H. R.; Yu, H. H., Advanced Materials 2011, 23, 4788. 8. Zhu, B.; Luo, S. C.; Zhao, H.; Lin, H. A.; Sekine, J.; Nakao, A.; Chen, C.; Yamashita, Y.; Yu, H. H., Nature Communications 2014, 5, 4523. 9. Yu, C.-C.; Ho, B.-C.; Hsiao, Y.-S.; Juang, R.-S.; Juang, R.-S.; Fang, J.-T.; Naidu, R. V. R.; Kuo, C.-W.; You, Y.-W.; Shyue, J.-J.; Chen, P.; Fang, J.-T., ACS Applied Materials & Interfaces 2017, 9, 30329. 10. Lin, H.-A.; Luo, S.-C.; Zhu, B.; Chen, C.; Yamashita, Y.; Yu, H.-h., Advanced Functional Materials 2013, 23, 3212. 11. Spain, E.; Keyes, T. E.; Forster, R. J., Biosensors & Bioelectronics 2013, 41, 65.

R.1.24
17:35
Authors : E. Perez, M. K. Mahadevaiah, E. Pérez-Bosch Quesada, P.S. Zarrin, Ch. Wenger
Affiliations : IHP - Leibniz Institut fuer innovative Mikroelektronik

Resume : Brain-inspired neuromorphic computing hardware technologies which could enhance the capability of computing systems to process large data sets with extreme efficiency are highly attractive. The short-term goal is to overcome the serious materials-based limitations that are preventing the exploitation of memristive elements as valuable new microelectronic components. By doing this, hardware accelerators for machine learning applications could be realized in the near future; while the long-term ambition is the establishment of spiking neural networks as truly disruptive computing hardware. To achieve this vision, memristive microelectronic devices should offer a number of important features, like: a controllable and predictable analogue response to stimuli; a response that incorporates hysteresis; the capability to change state reversibly a very large number of times; the ability to respond to pulsed stimulation; the ability to vary the dynamics of device response. All these issues must be achieved based on technology approaches which can be integrated in existing semiconductor manufacturing and processing techniques.

R.1.25
17:50
Authors : Thomas J. Webster, Art Zafiropoulo Chair
Affiliations : Department of Chemical Engineering, Northeastern University, Boston, MA 02115

Resume : There is an acute shortage of organs due to disease, trauma, congenital defects, and most importantly, age related maladies. While biotechnology (and nanotechnology) has made great strides towards improving tissue growth, infection control has been largely forgotten. Critically, as a consequence, the Centers for Disease Control have predicted more deaths from antibiotic-resistant bacteria than all cancers combined by 2050. Moreover, there has been a lack of biotechnology translation to real commercial products. This talk will summarize how nanotechnology with FDA approval can be used to increase tissue growth and decrease implant infection without using antibiotics. Studies will also be highlighted using nano sensors (while getting regulatory approval). Our group has shown that nanofeatures, nano-modifications, nanoparticles, and most importantly, nanosensors can reduce bacterial growth without using antibiotics. This talk will summarize biotechnology techniques and efforts to create nanosensors for a wide range of medical and tissue engineering applications, particularly those that have received FDA approval and are currently being implanted in humans.

R.1.26
17:50
Authors : Monica Marini1, S. Stassi,1 M. Allione,2 B. Torre,2 A. Giugni,2 M. Moretti,2 C.F. Pirri,1 C. Ricciardi,1 E. Di Fabrizio2
Affiliations : 1 Department of Applied Science and Technology (DISAT), Polytechnic of Turin, Italy 2 SMILEs Lab, PSE Division, KAUST, Thuwal 23955, Saudi Arabia

Resume : Super-hydrophobic surfaces (SHS) with regular patterns of micropillars were used to suspend DNA molecules and DNA after its interaction with other compounds such as protein, intercalants, and chemotherapeutic drugs. Briefly, a 5l droplet of a physiological-compatible solution containing nucleic acids and ligands is pipetted on the SHS. At temperature and humidity-controlled conditions the solution evaporates, and the droplet reduces the volume retracting from one pillar to the following one. The DNA helices follow this movement and the molecules linked to the top of one pillar are pulled to the next in line. With this technique we obtained free-standing self-assembled back ground-free DNA bundles, analysed by several techniques such as Raman Spectroscopy, high-resolution transmission electron microscopy (HRTEM), and Laser Doppler Vibrometer. The strategy shown allows studying the native characteristics of DNA helices and their alterations to the pristine conditions, showing a great potential in medical-oriented research.

R.1.27
 
ROUND TABLE DISCUSSION on FRONTIER RESEARCH Invited 7en minutes Key Speech : Organizer/Chair Dr. Valerio Voliani (Institute di Italiano Tecnologia,Pisa, Italy) (17.50-18.30)
17:50
Authors : Ch. Wenger
Affiliations : IHP - Leibniz Institut fuer innovative Mikroelektronik, 15236 Frankfurt / Oder, Germany

Resume : Recently, artificial intelligence reached impressive milestones in many machine learning tasks such as the recognition of faces, objects, and speech. These achievements have been mostly demonstrated in software running on high-performance computers. Novel CMOS approaches with in-memory processing is however more promising in view of the reduced latency and the improved energy efficiency. In this scenario, emerging memory technologies as resistive switching memory (RRAM), have been proposed for hardware accelerators of both learning and inference tasks. Latest results support RRAM technology as memristive synapses for in-memory hardware accelerators of machine learning.

R.1.28
17:50
Authors : Zhi Geng, Bo Zhu
Affiliations : Zhi Geng, Lecture, PHD, School of Materials Science and Engineering, Shanghai University, 99 Shangda Road, BaoShan, Shanghai, 200444, China, e-mail: gengzhi@shu.edu.cn Bo ZHU, Professor, PHD, School of Materials Science and Engineering, Shanghai University, 99 Shangda Road, BaoShan, Shanghai, 200444, China, e-mail: bozhu@shu.edu.cn

Resume : To realize the controllable design of the tubular surface structures, we try to figure out the possible formation process of the tubular functionalized PEDOTs during the electrochemical polymerization. By changing the component of the electrochemical solvent and the polymerization temperature, we found that the phase-separated water nanoparticles in the solvent directly induced the formation of PEDOT nanotubes. We further designed the explanation experiment and demonstrated that the phase-separated water nanoparticles act as templates and induce the formation of the nanotubes. To further explore the formation process of nanotubes in molecular level, we used glancing incident wide angle X-ray diffraction (GIWAXD) to measure the differences between nanodot and nanotube functionalized PEDOT thin films. We found that without adding any water in the electrochemcial solvent, the achieved PEDOT nanodot thin films showed face-on orientation. However, once we added little amount of water and formed the nanotube thin films, the crystal orientation would change to edgeon. Such phenomena would prove that the phase-separated water nanoparticles would act as soft templates and change the polymerization direction from parallel to vertical compared to the substrates. The achieved discovery would not only help us to further tune the morphology parameters on demand, but also expand the nanoassembling method to other two-phase system solvent via electrochemical deposition

R.1.29
17:50
Authors : Gonen Ashkenasy Nathaniel Wagner Indrajit Maity
Affiliations : Ben-Gurion University of the Negev, Beer Sheva, Israel

Resume : Like many other open systems in nature, living organisms are replete with rhythmic and oscillatory behaviour at all levels, to the extent that oscillations have been termed as a defining attribute of life. Recently, we have started to investigate the ?bottom-up? construction of peptide-based networks that display bistable behaviour and oscillations. We have utilized replicating coiled coil peptides, which have already served to study emergent phenomena in complex mixtures. In this talk, we will first describe the kinetic behaviour of coupled oscillators, producing various functions such as logic gates, integrators, counters, triggers and detectors.[1] These networks were also utilized to simulate the Kai-proteins circadian clocks, producing rhythms whose constant frequency is independent of the input intake rate and robust towards concentration fluctuations.[2] Then, we shall disclose our recent experimental results, showing that the peptide replication process also lead to bistability in product distribution out-of-equilibrium.[3-5] We propose that these recent studies may help further reveal the underlying principles of biological clocks, and pave the way to exploit synthetic oscillators for switching and signalling functions. 1. Wagner et al. J. Phys. Chem. Lett. 2015, 6, 60. 2. Gurevich et al. Chem. Commun. 2015, 51, 5672. 3. Mukherjee et al. Angew. Chem. Int. Ed. 2015, 54, 12452. 4. Wagner et al. ChemPhysChem 2017, 18, 1842. 5. Maity et al. Nature Commun. 2019, 11, 681.

R.1.30
17:50
Authors : Valerio Voliani
Affiliations : Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, P.zza San Silvestro, 12 - 56127, Pisa (PI), Italy.

Resume : Inorganic nanomaterials hold the promise to shift the current medical paradigms for the treatment and diagnosis of several diseases, among which neoplasms and infections. This is due to their peculiar chemical, physical and physiological behaviors. Despite the massive efforts, treatments based on inorganic nanomaterials are mainly at the preclinical stage, due to the body persistence issue. Indeed, non-biodegradable materials usually result in long-term persistence within excretion system organs, increasing their likelihood of toxicity. In this round table, the next strategies to support the translation of inorganic nanomaterials to the clinical settings will be discussed. The research leading to these results has received funding from AIRC under MFAG 2017 – ID 19852 project – P.I. Voliani Valerio.

R.1.31
Start atSubject View AllNum.
08:45 The Nobel Prize in Chemistry 2016 was awarded jointly to Jean-Pierre Sauvage, Sir J. Fraser Stoddart and Bernard L.Feringa “for the design and synthesis of molecular machines”    
09:45 Plenary Session (08. 45 – 09.45): Lecture by Professor Sir James Fraser Stoddart, Nobel Laureate in Chemistry (2016), Northwestern University, USA    
09:45 10.00 - Coffee Break    
 
YOUNG INVESTIGATOR FORUM Salutation to Nature : Invited Organizers/Chairs - PhD, Post-Doctoral Researchers Nanasaheb D.Thorat (Poland) and Katharina Brassat (Germany), PhD students Toshifumi Imajo (Japan) and Ana Arché Núñez (Spain), MS student Ting-Yu Lu (Taiwan)
09:55
Authors : Professor Insung S.Choi
Affiliations : KAIST, Korea

Resume : 2020 Young Investigator Forum - 107 Presentations on Frontier Research in Smart Nanomaterias, Nanosytems for a better life building and Human well-being

R.YIF.0
 
Frontier Research in Theranostics SESSION Invited Presenters : Organizer/Chair -Dr., Assistant Professor Nanasaheb D.Thorat (Wroclaw University of Science and Technology, Poland)
10:00
Authors : Nanasaheb D. Thorat, Joanna Bauer
Affiliations : Department of Biomedical Engineering, Faculty of Fundamental Problems in Technology, Politechnika Wroclawska, Wroclaw, Poland

Resume : Countless efforts are made by scientist to design different type of nanostructures, in the investigation for next generation cancer care applications. Various nanostructures often employ multimodality and show encouraging results at preclinical stage in cancer therapy. Specially designed smart nanostructures such as hybrid nanostructures are responsive to external physical stimuli such as light, magnetic field, electric, ultrasound, radio frequency, X-ray, etc are currently being investigated for cancer therapy. Thus, hybrid nanostructures provide a unique combination of important properties to address key challenges in modern cancer therapy: (i) an active tumor targeting mechanism of therapeutic drugs driven by a physical force rather than passive antibody matching, (ii) an externally/remotely controlled drugs on-demand release mechanism, and (iii) a capability for advanced image guided tumor therapy and therapy monitoring. The present report/talk cover various type of nanostructures that are developed by our group and those are responsive to magnetic and light-triggered modalities currently being developed for nonconventional cancer treatments. The physical basis of nanostructures and stimuli responsive modality is explained; so audience with a physics or, materials science background can easily grasp new developments in this field.

R.YIF.1
10:10
Authors : Grace Brennan1, Nanasaheb Thorat1,2, Martina Pescio1, Silvia Bergamino1, Joanna Bauer2, Ning Liu1, Tofail Syed1, Christophe Silien1.
Affiliations : 1 Modelling Simulation and Innovative Characterisation (MOSAIC), Department of Physics, School of Natural Sciences and Bernal Institute, University of Limerick, Limerick, V94 T9PX, Ireland. 2 Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 27, Wrocław 50-370, Poland.

Resume : Magnetic-plasmonic, Fe3O4-Au nanoparticles have been of interest in recent years, owing to their bifunctionality and biocompatibility. A plasmonic gold shell facilitates enhanced optical properties, with strong light scattering and absorption capabilities, facilitating bioimaging, surface-enhanced Raman and photothermal activity. Furthermore, thanks to its magnetic core, magnetic manipulation can be achieved which can be exploited in magnetic-field directed drug delivery, MRI contrast imaging and alternating magnetic-field hyperthermia. With great potential for clinical translation, it is important to uncover the physical properties these particles possess, which may help to optimise the stimuli applied to achieve the most efficient theranostics. Herein, superparamagnetic cores are functionalised with varying stages of gold growth from core-satellite structure to full gold shell growth. The optical extinction and single-particle scattering spectra of the various nanostructures are collected, exposing a spectral drift (up to 110 nm) or difference between the extinction and scattering maxima wavelengths. We explore how such a large drift may be of use in multimodal nanotheranostics, where photothermal therapy is conducted near the maximum absorption wavelength and imaging is carried out near the scattering maximum. COMSOL Multiphysics ® was used to create finite element analysis (FEA) models in wave optics and heat transfer to support the experimental findings. We also explore the effects of the gold shell on the magnetic properties of the multistage nanoparticles. This work was supported by Science Foundation Ireland (SFI) centre CÚRAM, the European Regional Development Fund (Grant Number 13/RC/2073), Marie Sklodowska-Curie Grant Agreement No. 751903. and CDA award 13CDA2221.

R.YIF.2
10:20
Authors : Erik Laurini, Domenico Marson, Suzana Aulic, Andrea Mio, Maurizio Fermeglia and Sabrina Pricl
Affiliations : MolBNL@UniTS Laboratory, Department of Engineering and Architecture, University of Trieste, Trieste, 34018, Italy

Resume : Theranostics is a new field of medicine which combines specific targeted therapies based on specific targeted diagnostic tests. The theranostic paradigm in cancer involves nanoscience to unite diagnostic and therapeutic applications to form agents for diagnosis, drug/gene delivery and treatment response monitoring. In this respect, our team as a part of a pan-European task force in the field has developed a series of innovative theranostic systems which proven to be excellent agents in cancer imaging and therapeutics in vivo. Nanotechnology-based imaging in cancer diagnosis plays a prominent role in both improving imaging sensitivity and specificity and reducing toxicity. Quite recently, based on the previous experience with Amphiphilic Dendrimers (AD) and further studies on AD modifications, we developed innovative nanosystems for positron emission tomography (PET) and single photon emission computed tomography (SPECT) imaging again exploiting AD-based self-assembly in which the surface of the amphiphile was decorated with different radionuclide (Gallium, Gadolinium and Indium) complexed within different macrocyclic chelator. The key findings of these efforts can be summarized as follows: the nanovectors were characterized by effective accumulation in tumors, exceeding sensitivity and specific imaging of various tumours, and was especially efficacious for tumors otherwise undetectable using the clinical gold reference. In addition, this nanovectors were endowed with an excellent safety profile and favorable pharmacokinetics. This study also demonstrated that nanotechnology based on self-assembling dendrimers can a fresh perspective for biomedical imaging and cancer diagnosis, i.e., cancer theranostics.

R.YIF.3
10:30
Authors : Dr. Ganeshlenin Kandasamy
Affiliations : Department of Biomedical Engineering, Vel Tech Rangarajan Dr. Sagunthala R&D Institute of Science and Technology, Chennai, Tamil Nadu, India

Resume : The induction of the heat by the magnetic nanoparticles (especially superparamagnetic iron oxide nanoparticles – i.e., SPIONs (i.e., Fe3O4/Fe2O3)) under the exposure of an alternating magnetic field (AMF) has a remarkable potential for being utilized in distinct non-invasive cancer nanotherapeutics such as magnetic hyperthermia therapy (or thermotherapy) and magnetically-triggered release of drugs for chemotherapy. But, the poor induction efficacies (estimated using parameters such as specific absorption rate (SAR) and/or intrinsic loss power (ILP)) of these SPIONs in the controlled environments have hampered their practical implementation so far. This could be mainly attributed to the significant lack of any of the following properties of the SPIONs: suitable size/shape, uniform aqueous dispersibility, biocompatibility, saturation magnetization and/or surface chemistry (for cell uptake/ functionalization). Therefore, it is highly desirable to prepare the SPIONs with the improved physicochemical, magnetic and biological properties for efficient nanotherapeutics. We have approached this via two different processes. In the first process, we have surface-engineered (individual or combined) (i) the unicore hydrophilic SPIONs via π-π conjugated carboxylic acid (COOH-)/amine (NH2-) based surfactants, and (ii) the multicore hydrophilic SPIONs (also called as iron oxide nanoclusters - IONCs) via ethylene glycol & ethanolamine based solvents in in situ manner through co-precipitation and/or thermolysis synthesis methods. It has been observed that these unicore/multicore SPIONs have possessed better properties including colloidal stability, and showed better efficacies (i.e., heat induction (SAR/ILP) & therapeutic) in the treatment of breast and liver cancers via thermotherapy. In the second process, we have encapsulated the as-synthesized hydrophobic SPIONs inside polymeric nanoparticles along with two diverse drugs – i.e., curcumin (a chemotherapeutic drug) and verapamil/nifedipine (calcium channel blocker (CCB) based drugs) to form multi-functional nanoparticles. The polymeric nanoparticles are made of poly(lactic-co-glycolic acid) (PLGA) in association with different stabilizers (i.e., polyvinyl alcohol (PVA) or d-α-tocopherol polyethylene glycol 1000 succinate (TPGS – vitamin E molecules)). Herein, the in vitro magnetically-triggered drug release studies have revealed that the application of AMF has effectually enhanced the discharge of the drugs, which have consequently improved the growth inhibition rates in the cervical and liver cancer cells via combined thermotherapy and chemotherapy (i.e., multimodal cancer nanotherapeutics).

R.YIF.4
10:40
Authors : Vijaykumar V. Jadhav, Pritamkumar V. Shinde, Rajaram S. Mane, and Colm O’Dwyer
Affiliations : School of Chemistry, University College of Cork, Cork, Ireland Department of Physics, Shivaji Mahavidyalaya, Udgir, India Center of Nanomaterials for Energy Harvesting/Generation and Storage Technologies, School of Physical Science, S. R. T. M. University, Nanded, Maharashtra, India

Resume : Hybrid nanostructures of metal, polymer, chalcogenide, oxide, hydroxide, nitride, and carbonaceous materials are believed to promise a great impact on the next generation of cancer diagnosis and therapy technologies. This chapter focuses on hybrid nanostructures that contain two or more distinct nanoparticles assembled in a functional structure that itself is still of nanoscale dimensions. This type of research aims to build a hybrid nanostructure whose medical effects are superior to those that could be realized from any simple mixture of the individual nanostructures. The unique characteristics of hybrid nanostructures in the nanometer range, such as high surface-area-to-volume ratio or shape/size-dependent optical properties, are drastically different from those of their bulk counterparts and hold pledge in the clinical field for disease therapeutics. This work deals with various kinds of novel hybrid nanostructures such as TiO2@Au, nanotube, silica, gold, and polymer that have demonstrated potential biogenic applications, especially in cancer research, including detection and treatments. Synergistic effects corroborated in these hybrid organic and inorganic nanostructures, useful for theranostics, are also explored in this article.

R.YIF.5
10:50
Authors : Niroj Kumar Sahu
Affiliations : Associate Professor, Centre for Nanotechnology Research, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India

Resume : Nanoparticle mediated chemotherapy for cancer is a potentially curative treatment modality that has been undergoing rapid technological improvements with the development of nanotechnology. Since chemotherapy is not fully effective at certain therapy resistance tumor cells, a combination of drug and heat can effectively exhibit a tumoricidal effect assuring selective attachment to target sites conserving surrounding healthy tissues. Nanomaterials are potential candidates to get functionalized for both chemotherapy and thermal therapy (hyperthermia) in a single system. Further, the dual drug delivery system using two drugs shows effective synergistic effects which enhances tumor regression capabilities compared to individual analog. In this regard, formulation of nano drug delivery systems by layer-by-layer (LBL) techniques employing dual drug loaded magnetite (Fe3O4) nanoparticles for synergistic chemothermal therapy has been investigated. The multifunctional mesoporous dual drug delivery system having magnetite with spherical morphology and high drug loading capacity shows sustained release in lysosomal and physiological pH. Polyphenolic drug increases the effect of DOX in MDA-MB-231 cells as well as repair the DOX induced cardiotoxicity. Thus this nano drug delivery system can be efficiently utilized for thermo-chemotherapy of cancer as well as to combat the cardiotoxicity induced by DOX which were investigated in vitro in H9C2 cells.

R.YIF.6
11:00
Authors : Enrico Catalano, Jürgen Geisler , Vessela N. Kristensen
Affiliations : Faculty of Medicine, University of Oslo (UiO), Oslo, Norway

Resume : Breast cancer is the second leading cause of death in women worldwide. The extremely fast development of metastasis and ability to develop resistance mechanism to all the conventional drugs make them very difficult to treat which are the causes of high morbidity and mortality of breast cancer patients. Nanotheranostics can be used for this purpose to implement the integration of diagnostic and therapeutic function in one system using the benefits of nanotechnology, with the purpose to treat cancer like one-size-fits-all scenario. The development of liposomes conjugated to superparamagnetic gold-coated iron oxide nanoparticles (Au-SPIONs) functionalized with an aptamer, to mimic natural antibody targeting, was implemented to allow target delivery of the anticancer drug of doxorubicin (DOX) on triple-negative breast cancer cells. Liposomes with iron oxide nanoparticles (SPIONs) and gold nanoparticles (GNPs) were prepared by ultrasound-assisted and controlled seeded growth synthetic methods, respectively. The aptamers AS1411, ERaptD4 and H2 were loaded by a desolvation cross-linking method and characterized by magnetic and physicochemical techniques. The synthesized nanoparticles were found to be spherical with an average diameter of 90 nm and zeta potential of about -49.4 mV. The level of cell death involved in the pathway of apoptosis, were measured to evaluate the synergistic effect of Au-SPIONs-mediated RF hyperthermia. MCF-7 and MDA-MB-231 human breast cancer cells were treated with different concentrations of Au-SPIONs. After incubation with NPs, the cells were exposed to RF waves (13.56 MHz; 100 W; 15 min). Cellular uptake of nanoparticles was confirmed qualitatively and quantitatively. The in-vitro anti-tumor effect of the designed delivery vehicle on MCF7 and MDA-MB-231 human breast cancer cells was evaluated by cell viability assay. The results obtained from cell viability and apoptosis evaluation showed that NPs and radiofrequency (RF) had no significant effect when applied separately, while their combination had synergistic effects on cell viability percentage and doxorubicin release and the level of apoptosis induction. The experimental results revealed that it could significantly inhibit the proliferation of cancerous cells. Aptamer-functionalized magnetic liposomes improved cellular uptake and efficiency to breast cancer cells as compared to non-targeting nanoparticles because of the high affinity of mentioned aptamer toward the overexpressed nucleolin and targeting of HER2 and ERα on MCF7 and MDA-MB-231 cell surface. The obtained results showed that the use of aptamer-functionalized magneto-plasmonic NPs in the process of hyperthermia and radiofrequency (RF) of cancer holds a great promise to develop a new combinatorial breast cancer therapy strategy.

R.YIF.7
11:10
Authors : Vishwajeet M Khot
Affiliations : Department of Medical Physics, Center for Interdisciplinary Research, D Y Patil Education Society (Institution Deemed to be University), Kolhapur-416006 (MS) India

Resume : Magnetic Hyperthermia Therapy (MHT) with magnetic nanoparticles (MNPs) has emerged as a potential treatment method for cancer either alone or in conjunction with chemo and radiotherapy. The major drawback during such treatment is to control the temperature and distribution of nanoparticles in vivo. In MHT, magnetic nanoparticles when subjected to an alternating current (AC) magnetic field heats the specific region of body or tissue [1]. Heat dissipation by MNPs is measured in terms of specific absorbance rate which in turn depends on magnetic moment, anisotropy energy, density, particle size and size distribution[2]. In the present talk, I will be discussing about synthesis, characterization, surface functionalization of nanocrystalline iron oxide based ferrites for MHT. Iron oxide (magntite) being a class of spinel ferrites due to its proven biocompatibility and good chemical stability is extensively studied and projected for its application in MHT. Other nanoferrites including cobalt ferrite, magnesium ferrite and mixed ferrites like Co-Zn ferrite etc have also been explored for their possible application in MHT [3]. To attain specific absorption rate at lowest particle concentrations, magnetic nanoparticles with uniform size distribution and high magnetic moment need to be synthesized. Tuning of magneto-structural properties will be achieved by choice of proper synthesis route. Once synthesized particle need to be surface functionalized with biocompatible polymers to improve colloidal stability in physiological media and cell compatibility. Finally, magnetic induction heating studies of these surface functionalized nanoferrites can be evaluated in vitro for their potential application as heating mediator in MHT for cancer. References: 1. V. M. Khot, A. B. Salunkhe, N. D. Thorat, R. S. Ningthoujam, and S. H. Pawar, Dalton Trans., 2013, 42, 1249 2. A.B. Salunkhe,, V.M. Khot and S.H. Pawar, Current Topics in Medicinal Chemistry, 2014, 14, 572-594 3. V.M. Khot, A.B. Salunkhe, J.M. Ruso, S.H. Pawar, Journal of Magnetism and Magnetic Materials 384 (2015) 335–343

R.YIF.8
11:20
Authors : Ashwini B Salunkhe
Affiliations : Department of Physics, Rajaram College, Vidyanagar, Kolhapur -416004, Email: vasusalunkhe@gmail.com

Resume : High magnetic moment Fe3O4 nanoparticles (NPs) are synthesized through simple co precipitation method by using new generation base Diisopropylamine (DIPA) which plays dual role as reducing agent and surface stabilizer. Spherical NPs with ~ 15 nm size and high magnetization value of about 92 emug-1 at room temperature are obtained by this novel method. High specific absorption rate value of ~717 wg-1is obtained for Fe3O4 NPs in water at an alternating magnetic field of 20kAm-1 and frequency of 267 KHz, which is attributed to high magnetization value. Magneto-polymeric micelle structure is formed by using Pluronic F127, anticancer drug Doxorubicin is conjugated in micelle by covalent linking with ligand molecules for magneto-chemotherapy. Finally, the magnetic resonance imaging (MRI) guided magneto-chemotherapy is achieved on breast cancer (MCF7) cells with ~ 96 % killing of cancer cells.

R.YIF.9
 
Smart Surfaces,Interfaces Frontiers SESSION Invited Presenters : Organizer/Chair - Dr. Katharina Brassat (Paterborn University, Germany)
11:30
Authors : K. Brassat, J. K. N. Lindner
Affiliations : Nanostructuring, Nanoanalysis and Photonic Materials Group, Dept. of Physics, Paderborn University, Germany; Center for Optoelectronics and Photonics Paderborn CeOPP, Paderborn, Germany; Institute for Lightweight Design with Hybrid Materials ILH, Paderborn, Germany

Resume : A vast variety of biosensors and devices for bio- and molecular electronics calls for ordered arrays of functional bioactive or biological nanoparticles arranged at high density and at predefined positions. One approach to create such arrays on large areas at low cost and under mild conditions is directed self-assembly (DSA), which allows to guide the deposition of entities on chemically or topographically prepatterned surfaces. We use block copolymer (BCP) lithography, a straight-forward upcoming nanolithography technique, to create arrays of guiding patterns for DSA with sub-20 nm sized features such as lines, dots or holes on surfaces of several materials on large areas. In this work, we exemplarily present two approaches to apply nanopatterns created by BCP lithography as templates to guide the arrangement of bionanoparticles into periodically ordered arrays. (i) Ferritin nanoparticles are site-selectively deposited from a colloidal suspension into hexagonally arranged nanopores; (ii) Melanin nanoparticles are site-selectively synthesized employing enzyme-functionalized nanopores as nanobatch reactors. The influence of surface wetting, zeta potentials and deposition/synthesis conditions on the nanoparticle arrangement are investigated by AFM, SEM and analytical TEM.

R.YIF.10
11:40
Authors : Monica Marini1, S. Stassi 1, M. Allione 2, B. Torre 2, A. Giugni 2, M. Moretti 2, C.F. Pirri 1, C. Ricciardi 1, E. Di Fabrizio 2
Affiliations : 1 Department of Applied Science and Technology (DISAT), Polytechnic of Turin, Italy 2 SMILEs Lab, PSE Division, KAUST, Thuwal 23955, Saudi Arabia

Resume : Recently, we used super-hydrophobic surfaces (SHS) to obtain suspended DNA molecules and bundles with a simplified physiological-compatible preparation procedure. A droplet of a saline solution containing nucleic acids is deposited over the SHS and at room temperature it spontaneously dehydrates and retracts from one pillar to the next in line without collapsing. The DNA molecules linked to the top of a pillar are pulled, following the drop movement: with this method we obtained free-standing self-assembled DNA fibers, studied by HRTEM [1,2], Raman Spectroscopy [3,4] and Laser Doppler Vibrometer [5]. The DNA-SHS platform allows characterizing native nucleic acids molecules and the variations to the pristine conditions as the ones occurring after the interaction with ligands such as intercalants, chemotherapeutic drugs and proteins by using different techniques and achieving quantitative information. This approach can find its application in medical-oriented research for personalized drugs administration (e.g., CisPt-DNA adducts), food safety (e.g., mycotoxins-DNA adducts), and the study of environmental pollutants effects on the double helix.

R.YIF.11
11:50
Authors : Yu-Ling Chang, Yu-Hsuan Chen, Hsuen-Li Chen, Dehui Wan
Affiliations : Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan (Yu-Ling Chang, Yu-Hsuan Chen, Dehui Wan); Department of Material Science and Engineering, National Taiwan University, Taipei, Taiwan (Hsuen-Li Chen)

Resume : Aluminum, with low price and high electric density, is the most abundant metal on earth. Besides, aluminum nanocrystal (AlNC) is expected as an alternative plasmonic material by adjusting its size and shape, leading to the shift of localized surface plasmon resonance (LSPR) band from UV-visible to near-infrared (NIR) regions. Previous research focused on the AlNC-based surface enhanced Raman scattering (SERS) detection throughout the UV region, while recent SERS substrates have been applied in the visible and NIR wavelengths. To facilely control the size and shape of AlNC and thus enhancing the electric field, the chemical synthesis has been reported but restricted to two dimensional (2D) SERS substrate. Therefore, herein, the chemically synthesized AlNC were introduced to develop a novel SERS substrate with low cost and high sensitivity based on inexpensive, lightweight, and disposable quasi-three-dimensional (3D) filter paper. The 3D feature of a filter paper can increase the number of hot spots and analytes in the z-axis direction of the depth of field, thus enhancing the sensitivity. On the other hand, the depth of field of a portable Raman spectrometer is generally larger than that of a microscopic Raman system, due to the simpler optical design. Hence, we believed that a portable Raman spectrometer might become an on-site detection tool to effectively provide higher sensitivity, if accompanying a sensitive 3D SERS substrate. In this work, we successfully synthesized AlNCs in an inert atmosphere based on the decomposition of aluminum precursors. The sizes ranged from 65 to 175 nm by simply controlling the ratio of the cosolvents. In order to prevent AlNCs from oxidation in the solution, a polydopamine (PDA) layer, which thickness was adjustable so as not to affect the electric field intensity, was self-assembled onto the surface of AlNCs. After that, droplets of AlNC@PDA solution and analyte solution were successively dropped onto a ?lter paper with pre-modified a hydrophobic layer. The hydrophobic property can condense the AlNCs and analytes to a small spot on the paper surface after air-drying. All Raman measurements were conducted with a portable 785-nm Raman spectrometer. The SERS signal was strongly enhanced as increasing density of AlNCs on a small contact area of the paper surface, indicating that the significant generation of SERS hot spots between the gaps of the aggregated AlNCs. For example, R6G is exploited as a Raman reporter with a LOD concentration of 10-6 M. More evaluations about the stability, reproducibility and real sample testing are still progressing and the details will be reported during the conference.

R.YIF.12
12:00
Authors : Jing Yu1, Minglun Li1, Xin Xu1, Jilei Wang1, Yan Cheng1
Affiliations : 1 School of Materials Science and Engineering, Nanyang Technological University, Singapore

Resume : The unique functionalities of polyelectrolyte brushes depend on several types of specific interactions, including solvent structure effects, hydrophobic forces, electrostatic interactions, and specific ion interactions. Subtle variations in the solution environment can lead to conformational and surface structural changes of the polyelectrolyte brushes, which is discussed mainly from a surface interaction perspective in this review. We highlight the use of these surface-grafted polymer films to create functional surfaces for various applications, including non-fouling surfaces, boundary lubricants, as well as stimuli-responsive surfaces. Based on the SFA and AFM methods, the impact factors for constraining the lubricity of polyelectrolyte brushes have been investigated. Experimental results reveal that the charged polymer chains and the osmotic pressure of the counterions can lead to wear resistance and low friction in monovalent salt solution. However, the lubrication can break down in the presence of multivalent counterions, even at very low concentrations, resulting from electrostatic bridging and brush collapse between chains from apposing surfaces and changes in surface topology. This study helps to give some insight into the interchain interactions contained in polyelectrolyte brushes but also can promote their application in many technical, medical, physiological, and biological areas. Additionally, we investigated the structure and antifouling properties of surface tethered zwitterionic peptide monolayers with different peptide chain lengths and charge distributions using a combination of surface plasma resonance (SPR), atomic force microscopy (AFM), and all atomistic molecular dynamics (MD) simulation techniques. Our results demonstrate that longer zwitterionic peptides exhibit better antifouling performance. The patchy charge distributions of the positive and negative charges in the peptides, although affecting the structure of the peptide molecules, do not significantly change the antifouling properties of the peptide monolayers.

R.YIF.13
12:10
Authors : Ji-Hyeon Kim, Ma Junfei, Seunghun Lee, Chang Su Kim
Affiliations : Advanced Nano-Surface Department, Korea Institute of Materials Science, Changwon 51508, South Korea

Resume : Infectious diseases and the deaths caused due to contact with germ-contaminated surfaces are severe problems worldwide. Antibacterial material based on silver nanowires (AgNWs) has a structural advantage when addressing this issue; this is because agglomeration is minimized when nanowires are fabricated into a film. Therefore, employing AgNWs for antimicrobial applications has garnered continuous interest, and increased research for further improvements has been observed. In this study, an AgNW film was fabricated onto glass by spin-coating and then subjected to surface irradiation up to a dose of 1200 kGy, using a low-energy electron beam (e-beam). This ?e-beam? irradiation changed the surface morphology and chemical composition; consequently, this improved the performance of the film. The generation of a silver (I) oxide (Ag2O) outer layer was identified over the AgNWs by X-ray photoelectron spectroscopy. The antibacterial test corresponding to a contact time of 1-h revealed that the e-beam irradiation increased the antibacterial activity from 93.5 to 97.3 % for Staphylococcus aureus, and from 96.1 to 99.9 % for Escherichia coli. Based on the experimental results and the known antibacterial mechanisms of silver (Ag) nanospecies, we discuss the method by which the antibacterial performance of the AgNW film was improved via the e-beam irradiation. This work provides a simple and swift method to functionally enhance the AgNW antibacterial film via e-beam irradiation.

R.YIF.14
12:20
Authors : Hayeon Kim, Eunji Lee*
Affiliations : School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea

Resume : Cryopreservation is very important in biotechnological, pharmaceutical, biochemical or food industries as the purpose of storage of protein drugs, cells, tissues and food, and ice slurries for refrigeration systems. Antifreeze protein (AFP) have been received attention with their potential as a cryopreservation agent by their ability to prevent the organisms from freezing at the subzero environment through antifreeze activity such as ice recrystallization inhibition or thermal hysteresis effect. However, it is struggling to apply the natural AFP in practical industries as cryopreservation agent because of their irreversible denaturation and the difficulty in extraction from nature. These challenges have led to developing artificial cryopreservation agents like dimethyl sulfoxide and sodium phosphates but due to the cytotoxicity and less biocompatible of them, the recovery rate of the target matter is too low when they are added. Here, the natural AFP mimetic short peptides conjugated with specific amino acids showing antifreeze activity and fibrous assembly with enhanced ?-? stacking are prepared by supramolecular chemistry to increase both antifreeze activity and biocompatibility. This research might provide a useful strategy to fabricate the cryopreservation agent through the supramolecular nanomaterials and to figure out the mechanism of ice binding to antifreeze protein.

R.YIF.15
12:30
Authors : Raquel Parreira, Ece Özelçi, Mahmut Selman Sakar
Affiliations : Institute of Mechanical Engineering, École Polytechnique Fédérale de Lausanne (EPFL)

Resume : Directed self-assembly of colloids capable of transducing remotely provided energy into mechanical motion may become instrumental for microfluidics technology and minimally invasive medicine. So far, magnetic torque has been the primary choice for the assembly and actuation of microdevices from ensembles of spherical or anisotropic microparticles. While proof-of-concept examples such as mixers, valves, and swimmers demonstrated the potential of the approach, the functionality of the devices engineered with bottom-up techniques is not comparable to the MEMS devices engineered using conventional top-down manufacturing methods [1, 2]. We developed a methodology that combines rotational motion induced by magnetic torque with contractile linear actuation provided by optomechanical nanogels on the same colloidal system. By combining thermocapillary forces with magnetic manipulation, we realized transient and permanent self-assembly of artificial microrobotic muscle that manifests multiple degrees of freedom actuation. Optomechanical actuation is provided by gold nanorods coated with a thermoresponsive polymer NIPMAM (N-isopropylmethacrylamide). Gold nanorods transduce incident light into surface-localized heat that drives fast (within milliseconds) and powerful polymer collapse (up to 50% strain) due to the transition of the polymer shell from hydrophilic to hydrophobic state above the lower critical solution temperature [3]. We introduced thin films of platinum and nickel around the gold core to augment the heat losses and drive convective flows that bring the particles together, introduce magnetization to the particle, and catalyse reactions that can lead to permanent engagement of particles. Adorning the surface of the particles with a repertoire of functional groups permits programmable self-assembly of complex devices from active and passive components, either through microfluidics or with direct laser writing. The assembly process can be re-initiated in the presence of free particles for sequential assembly and directed self-healing of defects. The presented methodology and selected materials are compatible with biological matter, paving the way for versatile and dexterous biomanipulation. References: [1] T. Sawetzki, S. Rahmouni, C. Bechinger, and D.W.M. Marr. PNAS (2008) 105:20141-20145. [2] A. Snezhko and I. S. Aranson. Nature Materials (2011) 10:698-703. [3] Berna Özkale, et al. Lab on a Chip (2019) 19:778-788.

R.YIF.16
12:40
Authors : Dias, L. F. G.(1)(2), Salz, D.(1), Noeske, P.-L. M. (1), Paulkowski, D. (1), Lisboa-Filho, P. N. (2)
Affiliations : (1)Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM, Germany (2)São Paulo State University ? UNESP, School of Science, Department of Physics, Brazil

Resume : Tailoring surface properties of materials for biomedical applications is important to avoid clinical complications. Exemplarily, depositing silver as an antibacterial component or immobilizing functional molecules on medical products are established procedures. (Borcherding et al., 2019; Bronze-Uhle et al., 2019; Ratner, 2013). Having in mind that, both performance and economic constraints need to be considered, we present two approaches for the surface modification of metal oxides sputtered-prepared and characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy and contact angle measurements. Following the strategy, octadecylphosphonic acid and etidronate will be immobilized according to previous work (Bronze-Uhle et al., 2019), and then after characterization the mono and bi phosphate surfaces will be compared, etidronate is a bisphosphonate commonly used to treat osteoporosis . In the frame of a second strategy, we considered immobilizing an organosilane containing quaternary ammonium, namely Dimethyloctadecyl[3-(trimethoxysilyl)propyl] ammonium chloride (DMOAP), from a methanolic solution on silicon wafers and titanium oxide. We obtained chloride-free hydrophobic film surfaces that were stable upon immersion in saline aqueous medium during one week. XPS investigations revealed an incomplete hydrolysis of compound on the surface.

R.YIF.17
12:50
Authors : Xiang YAO
Affiliations : State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China

Resume : Material cues to influence cell proliferation are a fundamental issue in the fields of Biomaterials, Cell Biology, Tissue Engineering and Regenerative Medicine. This report aims to investigate proliferation of single mammal cells on micropatterned material surfaces. To this end, we prepared cell-adhesive circular microislands with 20 areas on the non-fouling background, and systematically examined adhesion and proliferation behaviors of different kinds of single cells (primary stem and non-stem cells, cancer and normal cell lines) on micropatterns. Based on the analysis of experimental data, we found two critical areas about cell proliferation: (1) the critical spreading area of cells from almost no proliferation to confined proliferation, denoted as AP, (2) the critical spreading area of cells from confined proliferation to almost free proliferation, denoted as AFP. We further summarized the relative size relationship between these two critical areas and the characteristic areas about cell adhesion both on patterned and non-patterned surfaces. While proliferation of single primary cells was affected by cell spreading, those cell lines, irrespective of normal and cancer cells, did not exhibit significant cell spreading effects. As a result, this study reveals that proliferation of single cells is dependent upon spreading area, in particular for primary cells on material surfaces.

R.YIF.18
13:00 14:00 Lunch Break    
 
Invited Presentations : The 7en minutes Report on Frontier Research
14:00
Authors : Toshifumi Imajo, Takashi Suemasu, Kaoru Toko
Affiliations : Univ. of Tsukuba

Resume : Ge has higher carrier mobility than conventional mainstream material Si, and also has a low crystallization temperature (< 500 °C), which can be synthesized onto insulators under the heatproof temperature. Therefore, Ge on insulator technology has been widely studied for lowering the fabrication cost and improving the device performance. In particular, Ge-based devices fabricated on flexible plastic substrates will open up the possibility for developing advanced wearable devices such as multi-functional display. Solid phase crystallization (SPC) is a simple method to directly form polycrystalline Ge (poly-Ge) thin films on insulating substrates at low temperatures. Recently, we found that the control of the atomic density of an amorphous Ge precursor for SPC dramatically enlarged the grain size and updated the highest record of hole mobility of semiconductor thin film on insulator [1,2]. In this study, we applied this method onto plastic substrate. As a result, the SPC-Ge on a plastic substrate exhibited higher hole mobility than that of bulk Si. This result mean that single-crystal wafers are no longer necessary for fabricating a high-mobility semiconductor thin film. Besides, the resulting hole mobility 680 cm2/Vs is the highest value to date among those of semiconductor layers directly formed on insulators at low temperatures. This achievement will give a way to realize advanced electronic and optical devices simultaneously allowing for high performance, inexpensiveness, and flexibility. [1] K. Toko et al., Sci. Rep. 7, 16981 (2017). [2] T. Imajo et al., Applied Physics Express 12, 015508 (2019).

R.YIF.19
14:00
Authors : M. Marini,1,2 S. Stassi,1 M. Allione,2 B. Torre,2 A. Giugni,2 M. Moretti,2 P. Zhang,2 C.F. Pirri,1 C. Ricciardi,1 E. Di Fabrizio, 2
Affiliations : 1 DISAT, Politecnico di Torino, Corso Duca Degli Abruzzi, 24, 10129 Torino, Italy. 2 PSE Division, KAUST, Thuwal 23955, Saudi Arabia.

Resume : Recently we obtained free-standing self-assembled biomolecules fibers, by using a physiological-compatible preparation and super-hydrophobic surfaces (SHS). A droplet of the biomolecule solution is deposited over the SHS. At room temperature, the water in solution evaporates: the droplet decreases in volume and moves from one pillar to the next. With this method the molecules in solution are pulled and linked between micro-pillars. We suspended DNA, DNA/ligands, and cell membranes that were characterized by EM, Raman Spectroscopy and Laser Doppler Vibrometer. The DNA fibers allowed us obtaining background-free TEM direct images and measuring DNA bases and backbone without the use of contrast agents, with a resolution of 1.5 Å. TEM diffraction confirmed the quantities measured. Raman spectroscopy data expanded the EM data with structural/chemical information on DNA monomers, conformation and fluctuations related to the environment. DNA bundles were also used as ultrasensitive mechanical resonators to detect and study deviations to the native form, by administering intercalants and the chemotherapy cisplatin at increasing concentration. Alteration to sizes and Young’s modulus were successfully quantified. Overall, the results show that our approach can be applied to medical-oriented developments such as the optimal chemotherapy titration and the evaluation of the effects on DNA of pollutants and contaminants such as heavy metals.

R.YIF.20
14:00
Authors : Eunyoung Jeon (1) *, Joonseok Lee (1)
Affiliations : (1) Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Division of Nano and Information Technology, KIST School, University of Science and Technology (UST), Seoul, 02792, Korea

Resume : Conventional drug-eluting stents (DESs), biomedical implants, mainly use polymers or laser etching to load drugs. However, there are limitations in that polymers cause side effects such as late-thrombosis in the body and laser etching is complicated and expensive. Herein, we propose a porous nanostructured silica film for polymer-free DESs platform, which is synthesized by a sol-gel process using a catalyst, a silicon source, and structure-directing agents. As the structure-directing agents form micelles and grow on the surface, the porous nanostructured silica film is formed and has tunable pore sizes and channel lengths by adjusting reagents, molar ratios and reaction time. This suggests that a large amount of materials can be loaded, from large molecular weight molecules to small ones, and the films can be easily formed on the surface of commercial bare-metal stent. In addition, it is expected to be physically stable in the actual applications through pico-indentation experiment, expansion simulation and SEM, which evaluates the physical characteristics such as young's modulus (85.26 GPa) and hardness (2.69 GPa). Moreover, surface functionalization into hydrophobic with silane coupling agents (Octyltrimethoxysilane, Hexadecyltrimethoxysilane) allow the hydrophobic drugs to be loaded without polymers. The porous nanostructured silica film may represent an attractive platform that can be used in many other areas as well as polymer-free DESs.

R.YIF.21
14:00
Authors : Qun Ma, Pengcheng Gao*, Fan Xia*
Affiliations : Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences (CUG), 388 lumo Road, Wuhan 430074, R. P. China.

Resume : During the decades, widespread advances have been achieved on nanochannels correspondingly, including nanochannel based DNA sequencing, single-molecular detections, smart sensors, energy transfer/storage and so on. However, researchers focus all interests on the contribution from the functional elements (FEs) at the inner wall (IW) of nanochannels, little attention has been paid on the contribution from the FEs at the outer surface (OS) of nanochannels. Herein, we achieve explicit partition of FEOS and FEIW based on accurate regional-modification of OS and IW. Furthermore, the FEIW are served for ionic gating, and the chosen FEOS (hydrophobic or charged) are served for blocking interference molecules into the nanochannels, decreasing the false signals for the ionic gating in complex environments. Furthermore, we also define a composite factor, areas of a radar map, to evaluate the FEOS performance for blocking interference molecules.

R.YIF.22
14:00
Authors : Christopher Jay T. Robidillo,1,2 Jonathan G. C. Veinot1*
Affiliations : 1Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada 2Department of Physical Sciences and Mathematics, University of the Philippines Manila, Manila 1000, Philippines *Corresponding author

Resume : This study reports the ratiometric photoluminescent detection and quantification of the toxicologically potent organophosphate ester nerve agents paraoxon (PX) and parathion (PT) using red-photoluminescent silicon-based quantum dots (SiQDs) and the green fluorescent protein mAmetrine1.2 (mAm).1 The nerve agents selectively quench SiQD photoluminescence via a dynamic quenching mechanism mediated by organophosphate nitroaromatic groups. The method reported herein afforded micromolar detection limits for PX and PT and is unaffected by inorganic and organic interferents. In addition, paper-based sensors prepared from SiQDs and mAm have been successfully employed for the detection of PX and PT at low concentrations using a color analysis smartphone application. The sensor reported herein can potentially be used in the determination of PX and PT in actual samples. Reference: 1. Robidillo, C. J. T.; Wandelt, S.; Dalangin, R..; Zhang, L.; Yu, H.; Meldrum A.; Campbell, R.E.; Veinot, J. G. C. ACS Appl. Mater. Interfaces 2019, 11, 33478-33488.

R.YIF.23
14:00
Authors : H.Gogotsi (a), O.Ivanyuta (a),,E.Buzaneva (a),U.Ritter (b),P.Scharff (b)
Affiliations : (a) National Taras Shevchenko University of Kyiv,, 64, Volodymirska Str., 01601 Kyiv, Ukraine, oivanuta@gmail.com (b)TU Ilmenau, Institut für Chemie and Biotechnology, Postfach 100565, 98684 Ilmenau, Germany.

Resume : Here we report photoinduced electron transfer derivatives CNTs molecular films is easy to detect and electron transfer is sensitive to environment. The possibilities application derivatives CNTs are rotined in composition discrete sensory elements. Was investigational to influence of molecules such matters as an ammonia, alcohols, chlorine, and also to the factors of environment (temperature, humidity).

R.YIF.24
14:00
Authors : Viraj Bhingardive1,2, Guillaume Le Saux1,2 , Avishay Edri3, Angel Porgador3, and Mark Schvartzman1,2
Affiliations : Department of Materials Engineering1, Ilse Katz Institute for Nanoscale Science and Technology2, The Shraga Segal Department of Microbiology Immunology and Genetics Faculty of Health Sciences3, Ben-Gurion University of the Negev

Resume : Nanowires have been attracting great interest for numerous applications. In particular, their dimensions that are comparable to those of biomolecules, as well as their ultra-high aspect ratio and mechanical flexibility, make them very attractive for mimicking physiological environment of cells in the fundamental studies of the cell-environment intentions. To provide nanowires with the ability of chemical signaling they are often functionalized with signaling molecules. However, such a functionalization, based on either chemisorption or physisorption of biomolecules has not been site specific. Here, we took advantage of the fact that chemical-vapor-deposition grown nanowires carry on their tips metallic catalytic nanoparticles, to achieve the first of its type site selective functionalization of biomolecules. Such functionalization comes to provide a biomimetic 3D cell environment with precisely positioned biomolecules. We have grown Si nanowires from Au catalyst. We covered Au tips with biotinylated thiols, and used Neutravidin bridge to attach to the molecules of biotinylated anti-NKp30 – an activating ligands for Natural Killer cells, which are lymphocytes of the innate immune system. To study the effect of clustering at both nanometric and micron scale on cells, we investigated the effect of continues bed of nanowires verses micropatterned areas of nanowires. Further, we varied the stiffness of nanowires by changing their length and followed its effect on cell population.

R.YIF.25
14:00
Authors : Guillaume THOMAS,1 Laurent SCHLUR,1 Geoffrey GERER,1,2 Thomas COTTINEAU,2 Marc COMET,1 Valérie KELLER2 and Denis SPITZER1
Affiliations : 1 NS3E (Nanomatériaux pour les Systèmes Sous Sollicitations Extrêmes), ISL-CNRS-UNISTRA ? UMR 3208, French-German Research Institute of Saint-Louis, 5 rue du Général Cassagnou, B.P. 70034, 68301 Saint-Louis CEDEX, France; 2 Laboratoire des Matériaux Surface et Procédés pour la Catalyse (LMSPC) UMR 7515 CNRS/Université de Strasbourg, 25 rue Becquerel, 67087 Strasbourg, France;

Resume : Chemical Warfare Agents (CWA) and explosives detection with a high sensitivity and selectivity is crucial due to the growing threats from terrorist organizations to human health. Fast, selective and sensitive sensing methods are required to reduce the threat against CWAs. Microgravimetric sensor platforms with chemo or physico-selective interfaces offer an ideal solution to the previous drawbacks. We developed a bio-inspired nanostructured and functionalized cantilever for the detection of ultralow concentrations of explosives and simulants (DMMP (DiMethyl MethylPhosphonate) of CWA with different materials (TiO2, ZnO, CuO and Cu(OH)2) and morphologies (nanotubes or nanorods) in order to increase the surface area of adsorption and the limit of detection. This strategy of nanostructuration and functionalization promises significant improvements over existing sensors and for the next generation of cantilevers.

R.YIF.26
14:00
Authors : Ting-Yu Lu, Te-I Liu, Siou-Han Chang, Ming-Yin Shen, Hsin-Cheng Chiu Jiashing Yu
Affiliations : Department of Chemical Engineering, National Taiwan University Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Taiwan

Resume : The combination therapy, as an emerging strategy for improved clinical efficacy of cancer therapy, may not achieve effective response owing to the lack of highly selective and efficient tumor targeting. Herein, a dual stimuli-guided chemo/magnetothermal combination therapy system based upon histamine dodecyl carbamate (HDC)-coated doxorubicin (DOX)/magnetite-loaded solid lipid nanoparticles (SLNs) was developed for enhanced anticancer effects. Taking advantage of the dual pHe-induced electrostatic and magnetic guidance, the in vitro cellular uptake of these functionalized SLNs by TRAMP-C1 cancer cells was highly enhanced, leading to remarkably increased anticancer ability. With the highly selective delivery of the therapeutics toward tumor via the dual stimuli-mediated guidance, the effective growth inhibition of tumors with the small initial size (ca 50 mm3) by only chemotherapy was observed whereas the combination therapy was essentially required to fully inhibit the growth of large tumors (200 mm3). The IHC staining of tumor tissue sections with the combination therapy against large tumors showed the appreciable increase of tumor cell apoptosis and reduction of tumor angiogenesis. The results suggest that the dual stimuli-guided combination therapy system developed herein be prominent in fully inhibiting tumor growth even with the solid tumors of large size at the onset of the treatment.

R.YIF.27
14:00
Authors : Alina Vladescu1, Jürgen Schmidt2, Pinar Yilgor Huri3, Catalin Vitelaru1, Dorit Kloss2, Nesrin Hasirci4,6, Vasif Hasirci5,6, Lidia R. Constantin1, Mariana Braic1
Affiliations : 1National Institute of Research and Development for Optoelectronics - INOE 2000, 409 Atomistilor St., 077125, Magurele, Romania 2Innovent e.V., Jena, Prüssingstraße 27B, 07745 Jena, Germany 3Ankara University, Department of Biomedical Eng., Golbasi, Ankara, Turkey 4Near East University, Nicosia, Mersin 10, Turkey 5Acibadem Mehmet Ali Aydinlar Univ., Dept. Med. Eng., Istanbul, Turkey 6BIOMATEN, Middle East Technical University, Ankara 06100, Turkey

Resume : The growing implant market requires advanced Ti-based implants for long-term application, and for the newly engineered biodegradable Mg alloys with controllable dissolution rate s short-term applications. For this reason, Mg-alloys gain much more attention recently. Mg alloys are biodegradable material, but with too high corrosion rate and degradation occurs before the end of healing process. Moreover, during the corrosion, hydrogen released thereby causes pH increase of the surrounding tissue, inducing apoptosis and necrosis of tissue cells. For these reasons, it is a challenge to controlled degradation rate of Mg alloy and to provide sufficient time for the tissue to heal up to complete degradation of Mg implant. The aim of the present paper is to investigate different types of CaP coatings as possible candidate for temporary implants made of MgCa1 alloy. The coatings were prepared by magnetron sputtering and micro-arc oxidation methods. The coatings were investigated in terms of microchemical, microstructural and mechanical properties, corrosion resistance in SBF at 37°C, as well as the biocompatibility with human cell line. We acknowledge the support of the Romanian projects: no. COFUND-ERANET-RUS-PLUS-CoatDegraBac (no. 68/2018), within PNCDI III; Core Program-2020; no. 19PFE/2018 (PROINSTITUTIO).

R.YIF.28
14:00
Authors : Ph.D student Alexina Ollier1,2, Marcin Kisiel1, Urs Gysin1 and Ersnt Meyer1
Affiliations : 1 Institute of Physics, University of Basel, Switzerland 2 Swiss Nanoscience Institute, Klingelbergstrasse 82, 4056 Basel, Switzerland

Resume : Understanding nanoscale energy dissipation is nowadays among few priorities particularly in solid state systems. Breakdown of topological protection, loss of quantum information and disorder-assisted hot electrons scattering in graphene are just a few examples of systems, where the presence of energy dissipation has a great impact on the studied object. It is therefore critical to know, how and where energy leaks. Pendulum geometry Atomic Force Microscope (pAFM), oscillating like a pendulum over the surface, is perfectly suited to measure such tiny amount of dissipation, since a minimum detectable power loss is of the order of aW. Here, we report on a low temperature (T=5K) measurement of striking singlets or multiplets of dissipation peaks above graphene nanodrums surface. The stress present in the structure leads to formation of few nanometer sized graphene quantum dots (GDS) and the observed dissipation peaks are attributed to tip-induced charge state transitions in quantum-dot- like entities. In the real space the charging features are observed as Coulomb rings surrounding the GDS. The dissipation peaks and Coulomb rings strongly depend on the external magnetic field (B=0T-2T), the behavior we attributed to crossover from quantum dot carrier confinement to the confinement by magnetic field.

R.YIF.29
14:00
Authors : Paloma Lopes1,4, Xavier Moreira4, Paulo Brites1, Nuno M. M. Moura4, Ana T. P. Gomes3, M. Amparo F. Faustino4, Adelaide Almeida3, Paula Ferreira2, Manuel A. Coimbra4, M. Graça P. M. S. Neves4, Idalina Gonçalves1
Affiliations : 1CICECO – Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal 2CICECO – Aveiro Institute of Materials, Department of Materials and Ceramic Engineering, University of Aveiro, 3810-193, Aveiro, Portugal 3CESAM, Departamento de Biologia, Universidade de Aveiro, 3810-193 Aveiro, Portugal 4LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal

Resume : Porphyrinoid compounds have been incorporated into bio-based formulations as a strategy to develop photosensitive biomaterials suitable to be used in anti-infective strategies such as the photodynamic antimicrobial therapy. In this work, owing to develop a photosensitive carrier with biodegradable and biocompatible properties, potato starch films doped with porphyrins were developed. The influence of porphyrin’s dosage on the optical, photophysical, physicochemical, mechanical, and biological properties of the obtained starch films was evaluated. Porphyrins gave rise to transparent starch films changing from yellowish to reddish, depending on their native chemical structure. They also changed the films’ water tolerance and mechanical performance. Moreover, due to their ability of generating reactive oxygen species, the developed starch/porphyrin-based films inhibit the Gram-negative Escherichia coli bacterium growth. Therefore, the incorporation of porphyrins into starch-based formulations reveals to be a suitable strategy to develop newly photosensitive biomaterials with improved mechanical and water sensitivity performance. Acknowledgments: Thanks are due to the University of Aveiro and FCT/MCTES for the financial support of QOPNA research Unit (FCT Ref. UID/QUI/00062/2019) and of CICECO-Aveiro Institute of Materials (FCT Ref. UIDB/50011/2020 & UIDP/50011/2020). The authors acknowledge to POTATOPLASTIC project (POCI-01-0247-FEDER- 017938), financed by FEDER through POCI, “Isolago – Indústria de Plásticos, S. A.”, the project leader, and to “A Saloinha, Lda.” for providing potato byproducts. Nuno M. M. Moura thanks his research contract (CDL-CTTRI-88-89-97-ARH/2018) which is funded by national funds (OE), through FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in numbers 4, 5 and 6 of the article 23, of the Law Decree 57/2016, of August 29, changed by Law 57/2017, of July 19.. FCT is also thanked for the Investigator FCT program (Paula Ferreira, IF/00300/2015), for the Individual Call to Scientific Employment Stimulus (Idalina Gonçalves, CEECIND/00430/2017).

R.YIF.30
14:00
Authors : A.A. Romansky, V.L. Karbivskyy, V.А. Dubok, S.S. Smolyak, L.I. Karbivska
Affiliations : G.V. Kurdyumov Institute for Metal Physics of the N.A.S. of Ukraine

Resume : Previously, we developed samples of biocompatible composite based on calcium phosphates – "Syntekost" (synthetic bone). The aim of this work is to optimize the properties of bioactive materials for bone tissue recovery – ensuring their oncoprotective and anti-inflammatory properties. At various temperature conditions, a number of inorganic materials were synthesized: calcium phosphate ceramics, glasses and sitalls, as well as composites based on nanodispersed calcium hydroxyapatite. The effect of synthesis temperature conditions on the structure and properties of the obtained materials is investigated. Doping of a number of samples with bioactive elements was carried out with the aim of their functionalization. Samples of conductive bioactive nanocomposites based on nanodispersed apatite and nanodispersed graphite were obtained, and their properties were studied by electrophysical methods. An analysis of the XPS data of the obtained samples showed that, depending on the conditions of glass synthesis – melt cooling rate, temperature regime, and glass doping, the binding energy of the constituent elements changes. The temperature conditions of the process for producing such materials were optimized. The relative fraction of bioactive calcium phosphates and their crystallinity correlate with synthesis conditions, in particular – temperature regime. The XPS data for the fused glass sample indicate the presence of CaSiO_3 and Mg_2SiO_4, which are associated with bio-sitalls.

R.YIF.31
14:00
Authors : Susloparova, A.* (1), Mahdi, G. (1), Guérin, D. (1), Halliez, S. (2), Coffinier, Y. (1), Dargent, T. (1), Alibart, F.(1,3), Pecqueur S. (1).
Affiliations : (1) Univ. Lille, CNRS, Centrale Lille, Yncréa ISEN, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000 Lille, France (2) Université de Lille, Univ. Lille, Inserm, CHU-Lille, Jean-Pierre Aubert Research Centre (JPARC, UMR - S 1172), 59045 Lille, France (3) Université de Sherbrooke, Unité mixte internationale - Laboratoire Nanotechnologies & Nanosystèmes (UMI-LN2), Canada

Resume : Microelectrode arrays (MEAs) are widely used tools for investigating neural activity. To ensure the best sensitivity of the electronic devices to ionic signals and the lowest information loss, their electrochemical interface must be optimized by lowering their surface impedance, with materials that ensure the highest compatibility with the cells at the same time. Here, we show that by the electropolymerization of thiophene-derivatives, functionalized for higher cell biocompatibility and higher electrochemical performances, one can lower the microelectrodes’ surface impedance by the control of the polymer morphology. The microelectrode structuring with bottom-up grown conducting polymers was monitored in-situ by voltage-ramped impedance spectroscopy upon electropolymerization to track its circuit-elements modification. From screening of different electrochemical deposition conditions and morphological control by atomic force and electron microscopy, lower impedance values than commercial MEAs have been achieved, with performances comparable to spin-coated polymer electrodes. With the presented preliminary biocompatibility and stability tests, this study aims to demonstrate that unusual microfabrication techniques derived from electrochemistry can provide unique features at the material level to match properties of future emerging bioelectronics technologies to the strong requirements of sensing involving biological materials with rich material chemistry and morphology.

R.YIF.32
14:00
Authors : Ph.D student Martina Marsotto, Monica Dettin, Anj Zamuner, Luca Tortora, Giovanna Iucci and Chiara Battocchio
Affiliations : Department of Science, Roma Tre University, Rome, Italy

Resume : In the field of tissue engineering, a promising approach to obtain a bioactive, biomimetic, and antibiotic implant is the functionalization of a “classical” biocompatible material, for example, titanium, with appropriate biomolecules. For this purpose, we propose preparing self-assembling films of multiple components, allowing the mixing of different biofunctionalities “on demand”. In this context, the immobilization of Extracellular matrix proteins (ECM), such as fibronectin and vitronectin, that are known to promote osteoblasts adhesion, and therefore bone growth, is an interesting strategy in order to improve the osseointegration of titanium. Actually, the immobilization of an entire protein on a surface can present several drawbacks (protein denaturation and loss in the bioactivity), whereas the immobilization of short biomimetic peptides, reproducing the receptor binding site of the protein offers a convenient alternative route. Since osteoblast adhesion can take place by either interaction with RGD motif via cell membrane integrin receptors or interaction between cell membrane heparin sulfate proteoglicans and heparin binding sites on ECM, both RGD sequence (found in ECM proteins such as fibronectin and known to promote adhesion of several cell lines) and the peptide reproducing the 351-359 sequence of human vitronectin HVP (Human Vitronectin Precursor, amino acid sequence FRHRNRKGY), that increases osteoblast adhesion through the second specific mechanism, are expected to enhance osteoblast adhesion to titanium, the most used biomaterial for implants and prosthesis. Chitosan, on the other hand, is a versatile hydrophilic polysaccharide derived from chitin, with a broad antimicrobial spectrum to which Gram-negative and Gram-positive bacteria and fungi are highly susceptible, and is already known in the literature for the ability of its derivatives to firmly graft titanium alloys and show protective effects against some bacterial species, either alone or in combination with other antimicrobial substances such as antibiotics or antimicrobial peptides. In this context, we functionalized titanium surfaces with two different oligopeptides (RGD and HVP) chemically derivatized with chitosan (Chit-RGD and Chit-HVP), as well as, for comparison, with the two peptides alone. The chemical composition, molecular structure, and coverage quality of the obtained biofunctionalized surfaces were investigated by surface-sensitive techniques such as reflection−absorption infrared spectroscopy (RAIRS) and state-of-the-art synchrotron radiation-induced spectroscopies as X-ray photoemission spectroscopy (SR-XPS), and near-edge X-ray absorption fine structure (NEXAFS). Thanks to the so obtained complementary results, we were able to compare the two series of biofunctionalized surfaces, individuating the best candidate for the production of innovative bioactive materials.

R.YIF.33
14:00
Authors : Gerçem Altunordu, Ay?en Tezcaner, Zafer Evis, Dilek Keskin
Affiliations : Middle East Technical University; Biomaterials and Tissue Engineering Excellence Center

Resume : INTRODUCTION Bone tissue can be damaged by aging, some diseases or some accidents. Critical bone injuries and tissue losses have led to the need for the use of various biomaterials in the clinic. Firstly, metals such as cobalt-chromium alloys, stainless steel, titanium alloys have been used in biomedical applications. However, although these biomaterials support bone repair, they are not suitable for the purpose of supporting new bone formation, because they lack biological resorption characteristics. Therefore, many researchers oriented to other alternatives. One of these alternatives is the production of bioceramics such as hydroxyapatite (HA) and tricalcium phosphate. Many studies have shown that bioceramics containing silicon (Si), calcium (Ca) magnesium (Mg) have high bioactivity, biocompatibility, and strong mechanical properties. Therefore, they can be used in bone repair and regeneration as scaffolds with polymers such as polycaprolactone (PCL). The aim of study is to investigate potential of PCL/HA/Akermanite(AKR) and PCL/ carbonated hydroxyapatite (CHA)/AKR fibers produced by electrospinning method for bone tissue engineering. MATERIALS AND METHODS HA was synthesized by microwave irradiation method while CHA was synthesized by nanoemulsion technique. AKR was fabricated vis sol-gel method. After characterizations of bioceramics such as X-Ray Diffraction (XRD) and Fourier Transform Infrared (FT-IR), and bioactivity tests in simulated body fluid (SBF), scaffolds were producing by wet electrospinning. PCL using carrier material and was dissolved in ethanol. After optimization of some parameters such as voltage and flow rate, PCL/HA/AKR and PCL/CHA/AKR fibers were produced. Then, in vitro bioactivity tests of scaffolds were performed in SBF for 1,7 and 14 days to determine apatite layer formation. At the end of the immersion times, scaning electron microscopy (SEM) analysis was used for imaging apatite layers on the fibers. Also, elemental analysis (EDS) was done. Furthermore, cell adhesion, proliferation and osteogenic activity were studied with Saos-2 cells. CONCLUSION HA, CHA and AKR were synthesized respectively by microwave irradiation, nanoemulsion and sol-gel methods. Characterization techniques such as XRD and FT-IR proved that bioceramics produced successfully. In vitro bioactivity experiments showed that there are spherical apatite layer formations on the PCL/HA/AKR and PCL/CHA/AKR fibers produced by wet electrospinning. Moreover, cell proliferation and osteogenic activity of Saos-2 cells on the scaffolds were good.

R.YIF.34
14:00
Authors : Zhuangjian Liu, Jilei Wang, Jing Yu
Affiliations : School of Materials Science and Engineering, Nanyang Technological University, Singapore

Resume : Wearable electronic devices represent powerful tools towards long-term health-monitoring due to non-invasive measurement, lightweight and high comfort. To obtain accurate human physical information through the skin surface, the adhesion between the skin surface and devices is pivotal. However, it is still a great challenge for the epidermal electronic devices to achieve conformal attachment to the rough, soft and textured surface of the skin. This challenge is further amplified in hot and humid environments or during exercise, in which the sweat and motion of the human body may cause bonding failure (detachment) of many artificial pressure-sensitive adhesives. Herein we present a facile approach to fabricate a gecko-inspired, natural silk fibroin protein based adhesive for achieving highly conformal, comfortable, adjustable, and biocompatible adhesion on the skin surface. This micro-structured adhesive exhibits reliable and stable bonding force on skin surfaces, even under humid or wet conditions, and can be easily peeled off from the skin without causing significant pain. Such bio-inspired adhesive has a great potential to be applied as functional adhesives for various epidermal electronic sensors in the era of personalized healthcare.

R.YIF.35
14:00
Authors : Ahmaduddin Khan, Niroj Kumar Sahu
Affiliations : Centre for Nanotechnology Research, VIT, Vellore- 632014 (TN), India khan.ahmad4512@gmail.com, nirojs@vit.ac.in

Resume : Nanoparticles mediated drug delivery system has immense therapeutic applications especially in the field of cancer treatment. The chemo-therapeutic efficacy is enhanced when combined with radiation therapy or thermal therapy (Hyperthermia). For thermalchemotherapy, appropriate functionalised magnetic nanoparticles with desired magnetic parameter are pivotal. In this direction, we synthesised a highly aqueous dispersible PEG-dicarboxylic acid functionalised iron oxide (Fe3O4) nanocluster with superior magnetic properties by a simple solvothermal approach. The well distributed nanocluster (size~200nm) are formed by the assembly of smaller nanoparticles. Fe3O4 crystallizes in inverse spinel structure with crystallite size of 16.9 nm and possess mesoporous structure with high specific surface area of 71.3 m²/g. The carboxyl group was activated using hydrazine hydrate to form pH sensitive hydrazone bond which further was utilised to attach with doxorubicin (DOX) through C=O linkage. High drug loading efficacy was achieved due mesoporous nature of the nanoparticles and carboxyl group activation. This system shows higher and sustained release at pH 4.3 in comparison to normal physiological pH of 7.4 because of labile hydrazone bond. The synthesised Fe3O4 nanoclusters are biocompatible upto a concentration of 1mg as observed in vitro in MCF-7 breast cancer cell line. Due to higher saturation magnetisation of the nanocluster, a hyperthermic temperature of ~ 43 ºC was achieved in just 105 s at nanoparticle concentration of 2 mg/ml and at the applied AC magnetic field strength of 35.2 kA/m and frequency of 316 kHz. Hence, the PEGylated mesoporous Fe3O4 nanoclusters can be effectively utilised for thermochemotherapy of cancer.

R.YIF.36
14:00
Authors : Wenyi Hu* (1), Yuval Elani (2), and Claudia Contini (1).
Affiliations : (1) Department of Chemistry, Imperial College London, London, UK (2) Department of Chemical Engineering, Imperial College London, UK.

Resume : The current state of the art in artificial cell science relies on the construction of lipid vesicle membranes whose inherent properties, however, limit their applications particularly in sustaining cell internal biochemistry and communication between neighbors. Polymersomes are considered as ideal alternatives to liposomes because of their enhanced stability, chemical versatility, chemical and mechanical resistance. Nevertheless, the use of polymeric vesicles is restricted due to their low membrane permeability. Here, a suite of porous polymer membranes which is intrinsically permeable to molecules of defined sizes is explored as chassis for artificial cell, with the ideal functionalities of retaining specific molecules (e.g. protein and DNA) but allowing influx and efflux of small molecules (e.g. metabolites, signal molecules and mRNA). The porous vesicles are expected to form directly by self-assembly of copolymers, and the porosity is hypothesized to be controlled by varying experimental parameters during extrusion. The combinations of copolymers involve the mixtures of diblock copolymer PEG-PBD and triblock copolymer PEG-PPO-PEG with different molar ratios, and the solutions of two or more triblock copolymers PEO-PPO-PEO with different head-to-tail ratios. The porous polymer vesicle is novel in artificial cell science; thus, many potential applications can be tapped into, including for spatially-segregated biochemical reactions in polymer-based synthetic organelles.

R.YIF.37
14:00
Authors : Anita Visan1, Carmen Ristoscu1, Gianina Popescu-Pelin1, Mihai Soprony1, R. Cristescu1, Carmen Mariana Chifiriuc3, David Grossin4, Fabien Brouillet4, Ion N. Mihailescu1
Affiliations : 1 National Institute for Lasers, Plasma and Radiation Physics, Magurele, Ilfov, Romania ; 2 National Institute of Materials Physics, Magurele, Ilfov, Romania;3Department of Microbiology, Faculty of Biology, University of Bucharest, 060101; Research Institute of the University of Bucharest –ICUB, Spl. Independentei 91-95, Bucharest, Romania;4 CIRIMAT – Carnot Institute, University of Toulouse, ENSIACET; 4 Allée Emile Monso, 31030 Toulouse Cedex 4, France

Resume : We report on the successful deposition of antimicrobial chitosan-biomimetic nanocrystalline apatite –tetracycline thin films by Matrix Assisted Pulsed Laser Evaporation (MAPLE) using a KrF* excimer laser source (λ = 248 nm, ζFWHM ≤ 25 ns). Typical FTIR spectra of the obtained thin films were found to be highly similar to the spectrum of the initial powders. Scanning electron microscopy have evidenced a typical morphology characteristic to the deposition technique, advantageous for medical application, the nanoscale roughness increasing with the chitosan concentration. We have evaluated the antibacterial properties of the thin films containing chitosan and tetracycline deposited by MAPLE on titanium samples, using as model organisms both the Gram-negative E. coli and Gram-positive E. faecalis. The biocompatibility of the obtained films deposited on Ti substrates was evaluated by in vitro tests on human bone osteosarcoma cells. These tests have revealed the morphology and cellular cycle of the cells growing on the obtained thin films. The results have demonstrated that the chitosan- biomimetic nanocrystalline apatite-tetracycline composite thin films have improved the bone formation and further facilitated the anchorage between the bone and prosthesis, thus validating the MAPLE efficiency.

R.YIF.38
14:00
Authors : Yifei Luo, Wenlong Li, Qianyu Lin, Feilong Zhang, Sing Shy Liow, Ke He, Xian Jun Loh, Xiaodong Chen
Affiliations : Y. L.; W. L.; Dr. F. Z.; Dr. K. H.; Prof. X. C. Innovative Center for Flexible Devices (iFLEX), Max Planck ? NTU Joint Lab for Artificial Senses, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore, School of Materials Science and Engineering Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore. Y. L.; Q. L.; Dr. S. S. L.; Prof. X. J. L. Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 2 Fusionopolis Way, Innovis, Singapore 138634, Singapore.

Resume : Endogenous electrical signals regulate plant behaviors towards environmental changes. A deeper understanding of plant electrophysiology sets the foundation for plant-electronic hybridization. Hydrogels are the dominant material used for non-invasive plant electrophysiology measurement. However, conventional gels cannot electrically bridge electrodes with highly textured plants (especially hairy plants) effectively, due to lack of adhesiveness or conformability. Here, we show that surface-active thermogelling polymer can be used to establish an adhesive and conformable ionic interface between hairy plants and electrodes. The thermogelling polymer solution possesses drastic sol-gel transition near ambient temperature, which enables liquid-phase application for conformability to hairy plants. The surface-activity facilitates direct physical interaction between polymer networks and solid surfaces, enhancing interfacial adhesion. Such adhesive thermogel was demonstrated to allow for facile and high-fidelity electrical recording on highly hairy plants. It simplifies electrode setup and promises accessibility to a larger array of plant species, which will promote the development of plant-electronic hybrids towards smart sensing and modulating functions and have wider implications to on-human bioelectronics design.

R.YIF.39
14:00
Authors : Happy Agarwal, VenkatKumar Shanmugam
Affiliations : School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore – 632014, TN, India venkatkumars@vit.ac.in; drvenkatshanmugam@gmail.com

Resume : In the present study, we have biosynthesized zinc oxide nanoparticles (ZnO NPs) and investigated the mechanism of anti-inflammatory action of the nanoparticles using in vitro and in vivo models. In the in vitro model system, RAW 264.7 murine macrophages were pre-incubated with ZnO NPs and the effect of NPs on the lipopolysaccharide (LPS)-induced activation of nuclear factor-kappa B (NF- κB) pathway was evaluated. Results show that LPS mediated increase in expression NF- κB pathway was downregulated by an increase in expression of inhibitor of kappa B alpha (IκBα) in NP treated cells in a dose-dependent manner. The anti-inflammatory potential of ZnO NPs was also evaluated in Carrageenan induced mice paw edema model. Administration of ZnO NPs to mice, prevented Carrageenan induced mice paw edema and also suppressed the expression of pro-inflammatory mediators like interleukin-1 beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), and cyclooxygenase-2 (COX-2). Histological analysis of mice paw tissue section clearly indicated a reduction in leukocyte and neutrophil extravasation to the tissue space in mice paw that was pre-treated with ZnO NPs. Present findings give us an insight into the ZnO NPs mediated anti-inflammatory action.

R.YIF.40
14:00
Authors : Siaka Fadera, I-Chi Lee
Affiliations : Chang Gung University

Resume : The existence of cancer stem cells (CSCs) has been demonstrated in several solid tumors including brain cancer. These CSCs represent a sub-population of cells within tumor bulk that exhibit stem-cell-like characteristics and they are believed to play a significant role in tumor formation and recurrence, metastasis and they are resistant to conventional chemotherapeutic drugs. They are enriched in stem cells markers and markers associated with higher drug resistance and self-renewal. Chemotherapy is a well-accepted treatment modality for different cancer types including glioblastoma, however, its efficacy on glioblastoma still remains far from the best in most cases. The chemotherapeutic drugs are mostly effective on normal cancer cells leaving glioblastoma (GBM) stem-like cells insignificantly affected after treatment. Therefore, the establishment of better treatment strategy is needed in order to combat glioblastoma stem-like cells drug resistance. One such strategy is the induction of differentiation on GBM CSCs to normal cancer cells that will circumvent their self-renewal ability and improve their sensitivity to anti-cancer drugs. Previous literature has used low-intensity ultrasound (LIPUS) to inhibit the proliferation and induce apoptosis in cancer cells. Additionally, we have reported the induction effect of LIPUS on liver CSCs. In the present study, LIPUS was deigned to induce differentiation and concomitantly enhance the sensitivity and an uptake of drug by CSCs isolated from GBM cell line. We first designed and developed three dimensional tumor microenvironment using hyaluronic acid (HA) based polyelectrolyte multilayer (PEM) film system in order facilitate the isolation and enrichment of GBM CSCs. The next step is the use of LIPUS stimulation of GBM CSCs in combination with anti-cancer loaded gold nanoparticles. Colony formation, migration and invasion, putative CSCs marker expression, drug resistance, stem cell related genes expression and immunostaining were all investigated and compared with control group. The results revealed that high colony formation was observed on PEM system after 3 days of culture which continued through 7 days after culture. Higher expression of CD133, higher migration and invasion ability, up-regulation of proteins and genes related to CSCs and an increased drug resistance capacity were observed in cells isolated on PEM film system. From our previous study, LIPUS stimulation may result to down- regulation of CSCs colonies, down-regulation of CSCs related proteins and genes, down-regulation of migration and invasion ability, and an enhanced drug sensitivity of CSCs. Our proposed study design may provide alternative therapeutic strategy on the differentiation and effective combination therapy of glioblastoma CSCs.

R.YIF.41
14:00
Authors : Alina Sharova, Mario Caironi
Affiliations : Dipartimento di Fisica, Politecnico di Milano, P.zza L. da Vinci 32, 20133 Milan, Italy; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia, Via Pascoli 70/3, 20133 Milan, Italy

Resume : Going beyond the traditional concept of electronic devices, we convey the idea of making electronics edible. This unconventional approach exploits the electronic properties of natural and food-based materials for developing ingestible functional devices. Critical biomedical, pharmaceutical, and food industry applications are targeted by the proposed field. In this framework, we explore the potential of cost-effective and edible substance, honey, to be used as electrolytic gate viscous dielectric. Honey-gated organic field effect transistors (OFETs) based on both n & p type semiconductors are fabricated. A distinctive feature of these transistors is their long-term stability, reproducibility and low voltage < 1V operation in air. Devices exhibit forward-looking electronic performances, notably, electron and hole mobility–capacitance product of 3.5 × 10–3 μF/Vs and 23 × 10–3 μF/Vs, respectively, surpassing ones of the previously reported water-gated OFETs. Furthermore, the observed devices responsivity to humidity provides promising opportunities for sensing applications. We then demonstrate, for the first time, the implementation of honey-based integrated circuits: inverting logic gate and ring oscillator.

R.YIF.42
14:00
Authors : Soumya Menon, Dr.Venkatkumar S.
Affiliations : School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore 632014, India, soumya.menon1793@gmail.com

Resume : Cervical cancer is the most common malignancy suffered by women worldwide in the female reproductive system. The chemotherapeutic treatment using anticancer drugs are developed recently, but they lack delivery to the targeted tumour tissues. The commonly used anticancer drug paclitaxel is highly efficient against various cancer types, but due to its low solubility the enhancement of its property decreases. Recent advances includes nanotechnology based combinatorial drug delivery systems that offer enhanced pharmacokinetic property due the EPR (enhanced permeable retention), the larger surface area to volume ratio in nanoparticles, it also helps in adsorbing large amount of drug and act as a drug carrier. The combination of nanosystems aids in active or passive drug delivery through the cell membranes via endocytosis. In particular selenium nanoparticles is a well established chemotherapeutic agent due to low toxicity to the normal tissues, when compared to other nanosystems. The selenium nanoparticles act as both prooxidant in the presence of cancer tissues by producing ROS which eventually causes oxidative stress, mitochondrial damage, alter the genetic arrangements of the cancerous tissues, they are also major component of many antioxidant enzymes that also function at protecting from tumour like GSH (glutathione), selenomethioninecystien (recent established amino acid), theoredoxin (Trx) and so on. They act as antioxidant agent for normal tissues by scavenging ROS, regulating the DNA repair proteins, and protecting the damages caused by oxidative stress. In this paper more emphasis is done on the green synthesis of selenium nanoparticles to avoid the utilization of external harmful chemicals. The precursor used for the reduction of the sodium selenite which itself it a harmful chemical which was traditionally used as anticancerous agent, are Mucuna pruriens seed extract which is also a traditional medicine against various cancer types. The extract is used as a bioreductive catalyst which also helps in stabilization of the nanoparticle development. The biosynthesized SeNPs are optimized using BB design for evaluating the optimum particle size and process variable. The optimized SeNPs are then coated with chitosan which acts a biodegradable linker against anticancer drug paclitaxel. The combinatorial nanosystem is evaluated for its anticancerious activity against HeLa cancer cells. This innovative strategy can help in overcoming the drawbacks faced by chemotherapeutic drugs with low solubility, delivery to the targeted sites, highly biodegradable and low toxicity.

R.YIF.43
14:00
Authors : Priya Mullick1, Gopal Das2, and Aiyagari Ramesh1
Affiliations : 1Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, India, 2Department of Chemistry, Indian Institute of Technology Guwahati, India

Resume : Hydroxyapatite nanoparticles (HANPs) have elicited tremendous interest in recent times owing to their application potential in healthcare [1-5]. Herein, we report a biogenic route of hydroxyapatite nanoparticle (HANP) synthesis either in presence of the whole cell (WC) of the lactic acid bacteria (LAB) Lactobacillus rhamnosus GG or a malonic acid amphiphile (MA). HANPs were generated by in situ precipitation and the characteristic peaks at 3568, 1461, and 1041 cm-1 observed in FTIR analysis and the prominent peaks at around 2θ = 26° and 2θ = 33° in PXRD analysis revealed that the LAB cells and MA could generate hydroxyapatite phase. FESEM revealed that WC-HANPs were spindle-shaped and 70-110 nm in size while MA-HANPs were spherical and 22-27 nm in size. HRTEM indicated that the lattice distance for WC-HANPs and MA-HANPs was 0.28 nm and 0.29 nm, respectively, while SAED confirmed the crystallinity of HANPs. TGA analysis indicated degradation of WC-HANPs and MA-HANPs from 20°C to 800°C, with 83% and 87% residual mass, respectively. BET analysis indicated that WC-HANPs and MA-HANPs had a surface area of 170.47 m²/g and 90 m²/g, respectively, and an average pore size of 4.9 nm and 4.04 nm, respectively. Both the HANPs were non-toxic to cultured osteosarcoma MG-63 cells. The MG-63 cells grown in presence of HANPs appeared elongated, suggesting that HANPs likely support bone cell differentiation. The biogenic route of HANP synthesis outlined herein is an interesting prospect to generate potentially biocompatible nanomaterials for tissue engineering applications. [1] H. Cao et al., J. Phys. Chem. C 114,18352−18357 (2010).[2] Z. Xu et al., RSC Adv. 6, 76426−76433 (2016).[3] B. Palazzo et al., Adv. Funct. Mater. 17, 2180−2188 (2007).[4] H. Liu et al., J. Phys. Chem. C 115, 18538−18544 (2011).[5] P. Mullick et al., ACS Appl. Bio Mater. 2, 2927−2936 (2019).

R.YIF.44
14:00
Authors : Gi Doo Cha, Dae-Hyeong Kim
Affiliations : Center for Nanoparticle Research, Institute for Basic Science (IBS). School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University.

Resume : Implantation of biodegradable wafers near the brain surgery site to deliver anti-cancer agents which target residual tumor cells by bypassing the blood-brain barrier has been a promising method for brain tumor treatment. However, further improvement in the prognosis is still necessary. We herein present novel materials and device technologies for drug delivery to brain tumors, i.e., a flexible, sticky, and biodegradable drug-loaded patch integrated with wireless electronics for controlled intracranial drug delivery through mild-thermic actuation. The flexible and bifacially-designed sticky/hydrophobic device allows conformal adhesion on the brain surgery site and provides spatially-controlled and temporarily-extended drug delivery to brain tumors while minimizing unintended drug leakage to the cerebrospinal fluid. Biodegradation of the entire device minimizes potential neurological side-effects. Application of the device to the mouse model confirms tumor volume suppression and improved survival rate. Demonstration in a large animal model (canine model) exhibited its potential for human application.

R.YIF.45
14:00
Authors : Kyoung Won Cho and Dae-Hyeong Kim
Affiliations : Center for Nanoparticle Research, Institute for Basic Science Interdisciplinary Program for Bioengineering, Seoul National University

Resume : Industrial applications of anchorage-dependent cells require large-scale cell culture with multifunctional monitoring of culture conditions and control of cell behaviour. Here, we introduce a large-scale, integrated, and smart cell-culture platform (LISCCP) that facilitates digital mass culture of anchorage-dependent cells. LISCCP is devised through large-scale integration of ultrathin sensors and stimulator arrays in multiple layers. LISCCP provides real-time, 3D, and multimodal monitoring and localized control of the cultured cells, which thereby allows minimizing operation labour and maximizing cell culture performance. Wireless integration of multiple LISCCPs across multiple incubators further amplifies the culture scale and enables digital monitoring and local control of numerous culture layers, making the large-scale culture more efficient. Thus, LISCCP can transform conventional labour-intensive and high-cost cell cultures into efficient digital mass cell cultures. This platform could be useful for industrial applications of cell cultures such as in vitro toxicity testing of drugs and cosmetics and clinical scale production of cells for cell therapy.

R.YIF.46
14:00
Authors : Ting-Yu Lu, Te-I Liu, Siou-Han Chang, Hsin-Cheng Chiu*
Affiliations : Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan

Resume : Stimuli-responsive nanostructures have shown great promise for intracellular delivery of anticancer compounds. A critical challenge remains in the exploration of stimuli-responsive nanoparticles for fast drug release. Herein, ROS-responsive nanoparticles were rationally designed to effectively enhance drug release of radiosensitizer agents for synergistic thermo-radiotherapy. In this study, ROS-responsive poly(thiodiethylene malonate) (PSDEM) and PEG-PSDEM-PEG were synthesized and used as the main materials for ROS-responsive PSDEM nanoparticle (MNP) assembly in aqueous conditions. The radiosensitizer, suberoylanilide hydroxamic acid (SAHA), and the photothermal/photodynamic agent, indocyanine green (ICG), were encapsulated in the MNPs via hydrophobic interaction. The encapsulated ICG could generate not only heat but also ROS during NIR laser irradiation. In addition, via the ROS oxidation of the nonpolar sulfide residues in PSDEM into polar sulfoxide residues, the deformation of SAHA/ICG-loaded MNPs (I/S-MNPs) occurred, which this leading the SAHA release. The combination of NIR-triggered radiosensitizing and PTT effect could enhance the following therapeutic efficacy of radiotherapy, as confirmed via in vivo and in vitro evaluations. I/S-MNPs combined with radiotherapy showed the best tumor growth inhibition and the longest surviving time of mice, showing that I/S-MNPs exhibit a high potential for combination with radiotherapy to eliminate the malignant tumor.

R.YIF.47
14:00
Authors : Alesh Kumar and C. R. Mariappan
Affiliations : Department of Physics, National Institute of Technology, Kurukshetra 136119,India Registered email id: aleshkumar90@gmail.com

Resume : Bioactive materials are designed to interface with biological systems to treat, augment, or replace any tissue, organ, or function of the body. Among the different types of biomaterials, the mesoporous bioactive glass-ceramics (MBCs) are containing control amount of different ions with the aim of different activity like as antibacterial, osteogenesis and angiogenesis. Here we report the synthesis, characterization and bioactivity of different composition of silver containing MBCs. Bioactive glass-ceramics were synthesized by used CTAB. The introduction of Ag2O into the MBCs is intended to minimize the risk of microbial contamination through the potential antimicrobial activity of the leaching silver ions. The prepared samples were characterized by small angle X-ray scattering (SAXS), Fourier Transform infrared (FTIR) spectroscopy and high resolution transmission electron microscopy(HR-TEM). The SAXS patterns of samples show the agglomerates of ~15.3 nm average size with high polydispersity in size and size of the agglomerates varies from 2-40 nm range. FT-IR spectra show possible stretching and bending vibration modes of silicate and borate groups. HR-TEM confirms the mesoporous nature of MBCs. Bioactivity of MBCs was investigated by immersion of samples in simulated body fluid (SBF) at different time point followed by XRD and FTIR studies. The XRD patterns clearly show diffraction peaks of bone-like hydroxyapatite after immersion in DMEM. Silver-MBCs nanoparticles and there ionic dissolution extracts exhibited antibacterial effect against both positive and negative bacteria. Keywords:Bioactive glass-ceramics, Biocompatibility, Hydroxyapatite

R.YIF.48
14:00
Authors : Qianqian Niu, Shasha Lv, Huili Shao, Suna Fan*, and Yaopeng Zhang*
Affiliations : State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Belt and Road Joint Laboratory of Advanced Fiber and Low-Dimension Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China e-mail: zyp@dhu.edu.cn

Resume : Triboelectric nanogenerators (TENGs) convert mechanical energy into electrical energy, providing the possibility of self-powered implantable electronic devices. However, most implantable electronic devices still require an external power source. In addition, TENGs must have outstanding biocompatibility, biodegradability, and controllable degradation rate to prevent the need for a second surgery and avoid an inflammatory response. In this work, a silk nanofibril (SNF)-based bio-TENG is fabricated using a nascent SNF film (SNFF) and regenerative silk fibroin film (RSFF). To maintain the original meso/nanoscale structure of silk, SNFs with a thickness of 0.38 nm are directly exfoliated from natural silk. RSFF and SNFF have different microstructure and work functions. The output performance of the bio-TENG with the maximum voltage, current, and power density (PD) reach up to 41.6 V, 0.5 μA, and 86.7 mW/m2, respectively. The lifetime of the TENG depends on post-treatment of the RSFF package. The raw materials of the proposed TENG, including silk and magnesium (Mg), are completely biodegradable and biocompatible in vitro. With its high sensitivity and the ability to generate power from just a human pulse, the all silk-based bio-TENG may be an attractive power source for implantable self-powered electronic devices, such as pacemakers and implantable sensors.

R.YIF.49
14:00
Authors : Li Xu*(1), Yi-Ping Chen (2), Chung-Yuan Mou (1)
Affiliations : (1) Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan; (2) Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, No. 250, Wu Xinyi Street, Taipei 11031, Taiwan

Resume : Cancer is a growing public health concern of this century. So far, the major anti-tumor therapeutic approaches, including chemotherapy and radiotheray, still have a lot of limitations in clinical. Besides, it is well acknowledged that the tumor exhibits some mechanisms of immune suppression in the tumor microenvironment (TME) to evade immune surveillance. To overcome these problems, cancer immunotherapy has definitely grabbed the spotlight in recent years. Thereinto, cancer vaccine, especially ?in situ vaccination?, which enhances tumor immunogenicity via local applying of the immunomodulatory adjuvant is a promising option. Cyclic di-guanylate (c-di-GMP), an activator of the stimulator of interferon genes (STING), has been considered as a potential adjuvant in immunotherapy. It could induce the production of type I interferons (IFNs) via the STING-IRF3 pathway in antigen presenting cells (APC), resulting in enhancement of the tumor immunogenicity. However, the efficacy of c-di-GMP is limited by some inherent shortcomings, including the poorly membrane permeance, rapid clearance and the nonspecific distribution in body. So, we attempt to incapsulate the c-di-GMP with positively charged mesoporous silica nanoparticles (MSNs) to overcome its limitaions and induce enhanced antitumor immune response. The PEGylated RITC fluorescent MSNs modified with positively charged molecule (N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride, TA) were synthesized by co-condensation (labeled as RMSN-PEG/TA). The anionic c-di-GMP was loaded into RMSN-PEG/TA via electrostatic interactions (c-di-GMP@RMSN-PEG/TA). The loading amount of c-di-GMP on c-di-GMP@RMSN-PEG/TA was around 3 wt%. RAW264.7 cells treated with c-di-GMP@RMSN-PEG/TA obviously promoted the production of IL-6, IL-1?, and IFN-?, as well as the expression level of phospho-STING (Ser365) protein. The mouse 4T1 breast tumor-bearing Balb/c mice received intratumoral injection of c-di-GMP@RMSN-PEG/TA revealed dramatically tumor growth inhibition compared with the free c-di-GMP treated ones. In addition, the infiltration of activated CD11c+ dendritic cells and CD4+ T cells at the tumor microenvironment were detected by flow cytometry. By utilization of MSNs, we demonstrated that STING agonist c-di-GMP is a quite prospective adjuvant in cancer immunotherapy and MSNs are appropriate vehicles for ?in situ vaccination?.

R.YIF.50
14:00
Authors : Vincent Mauricio Kadiri,1,2 Claudio Bussi, 3 Andrew Holle, 4 Kwanghyo Son, 2 Hyunah Kwon,2 Gisela Schütz, 2 Maximiliano Gutierrez, 3 Peer Fischer 1,2
Affiliations : 1 University of Stuttgart, Institute of Physical Chemistry, 70569 Stuttgart, Germany 2 Max Planck Institute for Intelligent Systems 70569 Stuttgart, Germany 3 Francis Crick Institute, NW1 1AT London, United Kingdom 4 Max Planck Institute for Medical Research, 69120 Heidelberg, Germany

Resume : Applications in gene or drug delivery call for nanometer precision in the actuation of nanoscopic probes to navigate them reliably towards target tissues and eventually specific cells. While chemical targeting e.g. enables tissue-specific delivery, magnetically propelled nanomotors can navigate through complex biological media[1-2] and can transport material towards individual cells. However, commonly used magnetic materials are not biocompatible (Ni, Co) or possess weak magnetic moments (Fe, Fe3O4). Here, we demonstrate the first actively propelled nanomotors based on a rare-earth free hard magnetic system that is fully biocompatible while exhibiting magnetic moments that rival those of very strong permanent NdFeB micromagnets. We demonstrate cell targeting using gradient-free mT fields. The nanopropellers are fabricated with a physical vapour deposition technique termed glancing angle deposition (GLAD). The results establish biocompatible magnetic micromotors as promising tools for biomedical applications. 1. Wu, Z.; Troll, J.; Jeong, H.-H.; Wei, Q.; Stang, M.; Ziemssen, F.; Wang, Z.; Dong, M.; Schnichels, S.; Qiu, T.; Fischer, P., A swarm of slippery micropropellers penetrates the vitreous body of the eye. Science Advances 2018, 4 (11), eaat4388. 2. Walker, D.; Käsdorf, B. T.; Jeong, H.-H.; Lieleg, O.; Fischer, P., Enzymatically active biomimetic micropropellers for the penetration of mucin gels. Science Advances 2015, 1 (11), e1500501.

R.YIF.51
14:00
Authors : Deppie Brasinika, Anna Karatza, Dion Tsimourtos, Marco Scatto, Paolo Scopece
Affiliations : BioG3D - New 3D printing technologies, Lavrion, Greece; Nadir - Plasma & Polymers, Padova, Italy

Resume : Personalised orthotic devices that provide targeted support to weakened muscles and facilitate treatment of fractured bones, damaged joints or tendons are expected to usher in a new generation of patient-specific solutions without affecting active lifestyle. The latest advances in technologies of Additive Manufacturing allow for fabrication of highly customised designs with finely controlled structure in a fast and cost-efficient process. Utilising state-of-the-art 3D scanning processes, a 3D model of the unique human anatomy is reconstructed through reverse engineering. Subsequently, the bioinspired device design is optimised via Generative Design algorithms, considering the specific needs of each patient, e.g. areas in need of increased ventilation, or wound dressing, towards a thoroughly personalised medical solution. Taking advantage of innovative polymer materials and cutting-edge enhancing additives, smart functionalities, enhanced product strength and durability at lower overall weight are integrated in such devices. This is illustrated in the development of a 3D printed mutlimaterial wrist splint with antimicrobial and tailored mechanical properties using nanocomposite reinforced polymeric materials. By creating this product through Additive Manufacturing, a high level of personalisation is achievable, leading to predefined structures with tunable size and shape, thus allowing for increased breathability, making re-dressing wounds easier and daily tasks untroublesome.

R.YIF.52
14:00
Authors : Shuo-Hsiu Kuo, Po-Ting Wu, Ting-Yu Lu, Chih-Chia Huang, Jiashing Yu
Affiliations : Department of Chemical Engineering, National Taiwan University; Department of Photonics, National Cheng Kung University

Resume : Nanoparticle (NP) is widely used in biomedical application and cancer treatment due to the minute scale, multi-function, and long retention time. Among the various nanoparticles, the special optical property derived from localized surface plasmon resonance (LSPR) effect of metallic nanoparticles is a major reason that metallic nanoparticles are researched and applied. Copper nanoparticles (CuNPs) exhibit an absorption peak at the wavelength of 590 nm that can transfer the energy of photon to heat and rise the temperature of tumor microenvironment (TME), inducing cell apoptosis, which makes it a possible agent for photothermal therapy. Besides, compared to metals commonly used in cancer therapy such as gold and silver, copper has better biodegradability and is less likely to greatly accumulate in human body.On the other hand, iron nanoparticles (FeNPs), which have the potential to generate reactive oxygen species (ROS) via Fenton or Fenton-like reaction and produce oxygen in excess H2O2, are suitable for cancer treatment. Also, the magnetic property of FeNPs is appropriate for magnetic resonance imaging (MRI) to combine diagnosis and therapy. In drug delivery systems, the efficacy of traditional drugs is usually largely reduced after injection into human body due to their non-specificity, and side effects on normal tissues also emerge. Thus, through modificaiton of drugs on the surface of the FeNPs, when applying an external magnetic field, the nanoparticles could be guided to the targeted area, simultaneously reducing the amount of drugs used and minimizing injuries on normal cells. Also, it has also been proved that an externally applied magnetic field enhances the accumulation of magnetic nanoparticles at tumor sites, which in turn improves the curative efficacy of chemotherapy. Moreover, poly(styrene-co-maleic anhydride) (PSMA) could help increase drug solubility and stability, lengthening the half-life in blood plasma, and it can also improve the pharmacokinetic properties of the drugs. In our previous work, we found that PSMA could also serve as a successful drug carrier loaded with methylene blue (MB) via ?-? stacking and hydrogen bonding, indicating that it is a potential drug delivery platform. In this work, we synthesized dual metal nanoparticles, copper-iron nanoparticles (CuFeNPs), by a simple one-step hydrothermal reduction reaction. The Fe/Cu ratio of product could be controlled by manipulating the Fe/Cu ratio of reactant. The spinel structure of metal core was determined by XRD pattern. Moreover, the ROS generation ability of CuFeNPs was proved to be significantly higher than CuNPs. The magnetic property measured by hysteresis loop gave the potential of MRI agent. The photothermal effect was measured and the temperature was obviously elevated. The result indicated that CuFeNP is a potential agent for MRI and photothermal therapy.

R.YIF.53
14:00
Authors : Gabriela Moreira Lana, Judith Eubel, Rene Hensel, Eduard Arzt, Klaus Kruttwig
Affiliations : Gabriela Moreira Lana 1,2; Judith Eubel 1,2; Rene Hensel 1; Eduard Arzt 1,2; Klaus Kruttwig 1 1 INM - Leibniz Institute for New Materials 2 Department of Materials Science and Engineering, Saarland University

Resume : New adhesives for interaction with human skin are required for multiple applications. These range from wearable electronics to medical devices for diagnostics and therapy. Micropatterned dry adhesives are potential candidate materials allowing for a conformal contact and adhesion based on van der Waals interactions, dismissing the use of glues. At the same time they provide gentle but reliable long-term adhesion, as well as detachment without damaging delicate tissues. In this study, we developed high performance dry adhesives for biomedical purposes. The design consists of an array of elastic microfibrills with an aspect ratio of 3:1, terminated by a viscoelastic top layer of variable thickness. An advanced and robust method was developed to fabricate these film-terminated structures using medical-grade silicone. Systematic investigation of the adhesive properties regarding pull-off stress and work of separation was performed for epoxy substrates of different roughness, ranging from smooth (Rz=0.1 µm) to skin-like roughness (Rz=49.6 µm). The influence of hold time and pre-load for samples with different top layer thickness was evaluated in depth. Enhanced adhesion was observed for the microstructured samples compared to non-structured control samples on surfaces with skin-like roughness. For example, when applying 1.6 kPa compressive pre-stress for 60 seconds on substrates with skin-like roughness, samples with a top layer thickness of 30 µm reached up to approximately 7 kPa pull-off stress. On the contrary, the pull-off stress for the respective flat reference was approximately 1.7 kPa using the same conditions. The improved adhesive properties of the film-terminated adhesion system, particularly on surfaces with skin-like roughness, are attributed to the combined effect of the compliant soft film and the dynamic structural features of the fibrils, indicating the high potential of this adhesion system for skin device applications. Further investigations include a miniaturization of the system, for biomedical applications, e.g. treatment of tympanic membrane perforation. This work on dry adhesives displays a novel design for high performance adhesion systems for wearable electronics and wound dressing applications.

R.YIF.54
14:00
Authors : Micaela Matta, Alessandro Troisi
Affiliations : University of Liverpool, Department of Chemistry, Crown St., Liverpool L69 7ZD, United Kingdom

Resume : Organic bioelectronics is an interdisciplinary research area which involves the development of polymer semiconductors for nanomedicine and healthcare applications, such as wearable, implantable, diagnostic or sensing devices.1 Polymers are ideal candidates for bioelectronics, as their mechanical, physical and chemical properties can be tailored to match those of cellular tissues. A possible way to obtain biocompatible materials for bioelectronics is to exploit naturally occurring compounds, either alone or in composite materials. Eumelanin is an amorphous insoluble pigment present in both mammals and invertebrates. Eumelanin consists of amorphous aggregates of DHI (5,6-dihydroxyindole) and DHICA (5,6-dihydroxyindole-2-carboxylic acid) oligomers, interacting via ?- stacking and hydrogen bonding. Synthetic melanins are usually obtained using either DHICA or DHI and present a lower degree of structural disorder; they are being investigated for energy storage/production2 and as composite materials for biomedical applications.3 We present a systematic study of the electronic structure and conformational space of DHICA oligomers, characterized by a linear polymeric structure and strong antioxidant properties. Our computational insight not only fits well within existing experimental evidence, but can also predict the charge transport landscape in the bulk polymer.2 We then use molecular dynamics simulations to study the amorphous structures formed by this material, and investigate its aggregation-dependent excited state properties. Finally, we describe our approach towards the simulation of realistic DHI melanin aggregates using a combination of experimental evidence, statistics and molecular dynamics. (1) Rivnay, J.; Inal, S.; Salleo, A.; Owens, R. M.; Berggren, M.; Malliaras, G. G. Organic Electrochemical Transistors. Nat. Rev. Mater. 2018, 3 (2), 17086. (2) Kumar, P.; Di Mauro, E.; Zhang, S.; Pezzella, A.; Soavi, F.; Santato, C.; Cicoira, F. Melanin-Based Flexible Supercapacitors. J. Mater. Chem. C 2016, 4 (40), 9516?9525. https://doi.org/10.1039/C6TC03739A. (3) Migliaccio, L.; Altamura, D.; Scattarella, F.; Giannini, C.; Manini, P.; Gesuele, F.; Maglione, M. G.; Tassini, P.; Pezzella, A. Impact of Eumelanin?PEDOT Blending: Increased PEDOT Crystalline Order and Packing?Conductivity Relationship in Ternary PEDOT:PSS:Eumelanin Thin Films. Adv. Electron. Mater. 2019, 5 (3), 1?8. (4) Matta, M.; Pezzella, A.; Troisi, A. Relation Between Local Structure, Electric Dipole and Charge Carrier Dynamics in DHICA Melanin, a Model for Biocompatible Semiconductors, ChemRxiv 2019. https://doi.org/10.26434/chemrxiv.11323016.v1

R.YIF.55
14:00
Authors : Dimitrios A. Koutsouras, Leona V. Lingstedt, Katharina Lieberth, Jonas Reinholz, Volker Mailänder, Paul W. M. Blom, Paschalis Gkoupidenis.
Affiliations : Max Planck Institute for Polymer Research

Resume : PEDOT:PSS (Poly(3,4?ethylenedioxythiophene):polystyrene sulfonate)-coated electrodes have been widely used to evaluate the integrity of cellular barriers. Nevertheless, an experimental and systematic study of the correlation between tissue integrity and impedance of the sensing device has not yet been conducted. In this work, with the use of impedance spectroscopy, we investigate the way that the impedance ratio of the biological tissue to the recording device affects the recording ability of the latter. Various sized PEDOT:PSS-coated electrodes are employed and the effect of their dimensions to their sensing ability is determined. A simple equivalent circuit is proposed to model the biotic/abiotic ensemble and an analytical expression of the total impedance as a function of frequency is extracted. The study results in a critical impedance ratio of the biological tissue to the sensor, which allows for efficient sensing of the tissue integrity. This work opens new pathways for the realization of improved impedance?based biosensors with optimized sensitivity.

R.YIF.56
14:00
Authors : Emine Berber 1, Céline Muller 1, Julien Barthes 1, Nihal Engin Vrana 2*, Florent Meyer 1,3*
Affiliations : (1) Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, 11 Rue Humann, 67000 Strasbourg, France; (2)Spartha Medical, 14B rue de la Canardière, 67100 Strasbourg, France; (3) Faculté de Chirurgie Dentaire, Université de Strasbourg, 8 rue Sainte Elisabeth, 67000 Strasbourg, France

Resume : Biologic scaffolds composed of naturally occurring extracellular matrix (ECM) facilitate the constructive and functional tissue remodeling. Wharton's Jelly (WJ) contains significant amounts of ECM components and it`s a rich source of endogenous growth factors. Herein, we developed gelatin based porous scaffolds supplemented with WJ derived ECM micro-particles and Tannic acid (TA) by freeze-drying technique. TA was incorporated to the gelatin/gelatin-ECM hydrogels for antimicrobial and anti-biofilm purposes against Gram + and Gram- bacteria. Transglutaminase (TGA) was used to crosslink the composite hydrogels and to tailor their mechanical properties. Morphology and dimensions of the resultant scaffolds were characterized by scanning electron microscopy. Scaffolds with different pore sizes were obtained by adjusting the concentration of the gelatin solution. Ideal concentration was defined as 5.6 %v/w which resulted in 54.82 ± 24.6 µm pore diameter and 88.8 % porosity. In vitro cell culture results indicated that the WJ particles loaded porous gelatin scaffold facilitates the attachment and migration of macrophages and mesenchymal stem cells. Wharton?s Jelly derived micro-particles can be a strong candidate as a supplement to tissue engineering scaffolds for improving their functionality and biological activity. Key words: gelatin, Wharton?s jelly, tannic acid, extracellular matrix, scaffold, tissue engineering.

R.YIF.57
14:00
Authors : Hsien-Yeh Chen
Affiliations : Department of Chemical Engineering, National Taiwan University

Resume : Functionalized poly(p-xylylenes) can be deposited via chemical vapor deposition (CVD) polymerization to generate ultra thin films as conformal coatings and, due to the pre-defined chemical functionalities, provide a flexible solution to surface engineering challenges as they decouple surface design from bulk properties. Hence, the technology comprises essentially a one-step coating procedure to generate functionalized surfaces without requiring any kind of post-treatment once the films are deposited. The CVD-based polymer films can be generically applied to a wide variety of substrates and establish a reactive interface that allows for further modification. The simplicity in providing a wide range of functional groups, the excellent adhesion to various substrates, and its applicability to devices with three-dimensional geometries are key advantages when compared to polymers deposited by solvent-based methods. Related applications have been shown to provide engineered interface properties to resist bacteria/biofilm formation, to prevent non-specific adsorption of proteins and cells (antifouling), and to manipulate cellular activities including proliferation, differentiation, and spheroids formation for a range of cells and stem cells. Furthermore, the coating technology has used for the encapsulation of liquids and to result in a revolutionary production of a intraocular lens (IOL), and the new IOL ehxibited: (i) enhanced compatibility and stability to avoid leaching of potential harmful substances to the surrounding biological environment and (ii) customizable optical and biological properties for diverse patient needs. Finally, instead of forming conventional thin films, the vapor deposition of the poly-para-xylylene polymers on a dynamic substrate, i.e., sublimating ice, was found to produce three dimensional bulk materials and composites with controllable porosity and size.

R.YIF.58
14:00
Authors : Federico Cerrone*(1),Tatyana Pozner(2), Paolo Ceppi(3), Murugan Rajendiran(4), Shu Wenting(5), Eoin O?Cearbhaill(5) Brian J. Rodriguez(6) Kevin O?Connor(7).
Affiliations : *(1) School of Biomolecular and Biomedical Science, University College Dublin, Belfield Campus Dublin, Ireland (2) Department of Stem Cell Biology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany (3) IZKF Junior Research Group I, Interdisciplinary Center for Clinical Research, University Hospital Erlangen, Erlangen, Germany (4) Polymeric Materials and Nanocomposites group, School of Physics, Trinity College Dublin, D2, Dublin, Ireland (5) School of Mechanical and Materials Engineering, University College Dublin, Dublin, Ireland (6) School of Physics, University College Dublin, Dublin, Ireland (7) BEACON SFI Bioeconomy Research Centre, O?Brien Science Centre, University College Dublin, Dublin, Ireland

Resume : Efficient neurite outgrowth, mechanoresponse and the increasing of the survival rates are critical for the successful cultivation of cortical neurons in vitro[1] and the potential of central nervous system regeneration in vivo, after maturation of these cells[2]. Polyhydroxyphenylvalerate (PHPV), an aromatic polyester of bacterial origin[3], was electrospun into nanofibers and blended with polycaprolactone (PCL) and electrospun in nanofibers for use in a 3D (CellCrown?) configuration and in a 2D coverslip coated configuration. Contact angle and nanoindentation tests by atomic force microscopy (AFM) showed that PHPV has a higher hydrophilicity and adhesion when compared to pure PCL. Height images derived by AFM scan identify a mixed roughness/smoothness ratio of the PHPV/PCL blend, where PHPV is responsible for the smoother component while PCL gives the roughness component of the blend. Nanoindentation tests of the electrospun fibers, done by AFM, exhibit correlated force-map curves that can identify the Young?s modulus of the materials[4]. PHPV, other than increased adhesion, has a more viscoplastic behaviour in the stress/strain response (where the plastic modification of the material is evident at an earlier stage than PCL) but is too soft and show poor mechano-response for cell cultivation. PCL has a strain hardening behaviour under stress[5] and higher elasticity, but it is hydrophobic and too tough and stiff, hindering cell survival. PHPV/PCL shows a superior behaviour compared both to PCL and PHPV. The same rheological results have been confirmed by stress/strain tests at macroscale by Universal Tensile Machine (UTM); PHPV/PCL has a measured stiffness and an elastic modulus 2.3 and 2.4-fold lower than PCL, respectively. The PHPV/PCL blend maintains the mechanical solidity but having a higher hydrophilicity, softness and a relatively low stiffness can sustain for longer the attachment of neuronal cells, and improves their adhesion and their neurite elaboration, facilitating synaptic contacts. In facts, electrospun PHPV/PCL allow a 2.3-fold increase in the life-span of human induced pluripotent stem derived cortical neuronal cells (hiPS) compared to pure PCL fibers. HiPS-derived cortical neuronal cells grown on PHPV/PCL fibers show a 3.8-fold higher cumulative neurite elaboration compared to neurites grown on PCL fibers only. 96% of cortical neuronal cells die after 8 days of growth when plated on PCL fibers alone while more than 83% and 55% are alive on PHPV/PCL fibers on day 8 and day 17, respectively[6]. An increased migration rate of cortical neuronal cells is also promoted by the blend compared to the PCL fibers alone. The critical survival rate improvement of hiPS derived cortical neuronal cells on PHPV/PCL blend holds promise in using these biocompatible nanofibers as implantable materials for regenerative purposes of an active cortical neuronal population after full maturation in vitro[6]. References [1] D.E. Koser, A.J. Thompson, S.K. Foster, A. Dwivedy, E.K. Pillai, G.K. Sheridan, H. Svoboda, M. Viana, L.D. Costa, J. Guck, C.E. Holt, K. Franze, Mechanosensing is critical for axon growth in the developing brain, Nature Neuroscience 19(12) (2016) 1592-1598. [2] W. Ma, T. Tavakoli, E. Derby, Y. Serebryakova, M.S. Rao, M.P. Mattson, Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells, Bmc Developmental Biology 8 (2008). [3] K. Fritzsche, R.W. Lenz, R.C. Fuller, AN UNUSUAL BACTERIAL POLYESTER WITH A PHENYL PENDANT GROUP, Makromolekulare Chemie-Macromolecular Chemistry and Physics 191(8) (1990) 1957-1965. [4] N.D. Wanasekara, S. Ghosh, M. Chen, V.B. Chalivendra, S. Bhowmick, Effect of stiffness of micron/sub-micron electrospun fibers in cell seeding, Journal of Biomedical Materials Research Part A 103(7) (2015) 2289-2299. [5] Q.H. Liu, S.C. Yuan, Y.H. Guo, A. Narayanan, C. Peng, S.J. Wang, T. Miyoshi, A. Joy, Modulating the crystallinity, mechanical properties, and degradability of poly(epsilon-caprolactone) derived polyesters by statistical and alternating copolymerization, Polym Chem-Uk 10(20) (2019) 2579-2588. [6] F. Cerrone, T. Pozner, A. Siddiqui, P. Ceppi, B. Winner, M. Rajendiran, R. Babu, H. S. Ibrahim, B.J. Rodriguez, J. Winkler, K.J. Murphy, K.E. O?Connor Polyhydroxyphenylvalerate/polycaprolactone nanofibers improve the life-span and mechanoresponse of human IPSC-derived cortical neuronal cells. Material Science Engineering C. Accepted

R.YIF.59
14:00
Authors : Jiuk Jang, Hyobeom Kim, Jang-Ung Park
Affiliations : Department of Materials Science and Engineering, Yonsei University, Seoul 03722, Republic of Korea; Center for Nanomedicine, Institute for Basic Science (IBS), Seoul, 03722, Republic of Korea

Resume : Blindness is one of the most severe problems that degrade the quality of human life, rather than any other disorders. Various methods for restoring the vision have been studied. Among them, electronic prosthetic devices for a visually-impaired person allows the individual to recognize the phase and movement of an object. It is based on the principle of converting an external light signal into an electric signal that is transmitted to the human optic nerve, which allows a person to perceive the electric signal as light. This retinal prosthesis has undergone considerable development over the last few decades; however, there are several challenges to be solved. First, the resolution is too low to visualize the information from the outside to human eye. This problem is mainly originated from the high impedance of the electrodes. Second, the mechanical modulus of the material within the device is a mismatch to the modulus of the retina tissues, followed by severe inflammation and permanent damage to the retina. Finally, since this device is planned to be implanted on the human eye, the reliable operation is important. Here, we presented the high-resolution, flexible retinal prosthesis based on three-dimensional (3D) soft stimulation electrodes for blind people. The high-resolution phototransistor array with a stimulation electrode allows the patient to perceive the image clearly. 3D structure of the electrode reduces the impedance of each pixel so that the single-pixel can be integrated into high density without any electrical breakdown. Since we formed 3D electrodes using soft, self-healable materials, this stimulation electrode hardly damages the retina surface and easily restore their electrical characteristics under various mechanical deformations, suggesting the reliable operation of the implanted retinal prosthesis. Furthermore, in-vitro and in-vivo animal experiments using mouse and rabbit provided enough biocompatibility for the human trial. This optoelectronic retinal prosthesis using high-resolution, flexible transistor arrays provides a substantial chance to step-up for next-generation implantable electronic devices.

R.YIF.60
14:00
Authors : Paola Pellacani (1,2), Rodrigo Calvo (1)*, Carlo Morasso (3), Dario Gallach (1), Lucia Fornassari (1), Franco Marabelli (2) and Miguel Manso Silvan (1).
Affiliations : (1)*Departamento de Física Aplicada and Instituto de Ciencia de Materiales Nicolás Cabrera, Universidad Autónoma de Madrid, 28049, Madrid, Spain;(2)Plasmore S.r.l., Via Riviera 12b, Pavia, Italy; (3)ICS Maugeri, Pavia 27100, Italy

Resume : Sequential processes combined with specific lithographic methods allow the fabrication of advanced materials structures. In the present work we have used self-assembled colloidal monolayers as lithographic structures for the conformation of ordered Si submicrometer pillars by reactive ion etching. We have explored different discharge conditions to optimize the Si pillar geometry. Selected structures were further decorated with gold by conventional sputtering, prior to lift-off of the colloidal monolayer. The resulting structures consist of a gold crown, that is, a cylindrical coating on the edge of the Si pillar and a cavity on top. We have analysed the Au structures in terms of electronic properties by using X-ray absorption spectroscopy prior and after post-processing with thermal annealing at 300ºC and/or interaction with a gold etchant solution (KI). The angular dependent analysis of the plasmonic properties has been studied by Fourier transformed UV-vis measurements. Particular conditions have been selected to perform a SERS evaluation of these platforms with two model dyes, prior to confirming the potential interest for the well-resolved analysis of biomolecules of several tens of KDa.

R.YIF.61
14:00
Authors : Duenas-Ramirez, P.* (1), Juillot, M. (2), Pillods, F. (3), Pichon, B. (1), Carton, A. (1), Zaloszyc, A. (3), Choquet, P. (3), Mertz, D. (1), Bégin-Colin, S. (1)
Affiliations : (1) Institut de Physique et Chimie des Matériaux (UMR CNRS-UdS 7504, University of Strasbourg), France. (2) Ecole Européenne de Chimie Polymères et Matériaux (University of Strasbourg), France. (3) 2. Imagerie Préclinique?UF6237, Pôle d?imagerie, (Hôpitaux Universitaires de Strasbourg), France.

Resume : The use of functionalized nanoparticles (NPs) for pollutant removal applications in a biological environment emerged recently as an important topic. Nowadays, studies have shown the promising capacity of magnetic iron oxide (IO) nanoparticles for removing of pollutants from water or human body. Magnetic nanoparticles can be easily separated from water under an external magnetic field. Moreover, they may have a high surface-area that allow high removal capacity of micro-pollutants with a small quantity1. In that context, we have designed iron oxide nanostructures to improve the phosphate removal during the peritoneal dialysis process. At first, we have developed the synthesis of magnetic raspberry shaped nanostructures (RSN) by modified-polyol solvothermal methods2,3.This IO nanostructures consist in orientated aggregates (150-500 nm) of nanocrystals (10-25 nm) which preserve them from oxidation and provide a very high saturation magnetization (Ms~85 emu/g). In addition, they display a superparamagnetic behaviour favouring the colloidal stability. We have investigated the adsorption of phosphates using these RSNs and demonstrated that they can be used as phosphate recyclable adsorbents, extracted from the media by applying a magnet. In vitro experiments have been conducted in a home-made peritoneal dialysis model to evaluate the efficiency of dialysis performed model. 1. Chem. Mater. 19 (2007), 4494?4505 2. Chem. Eng. J. 256 (2014) 187?204 3. Nanoscale (2017), 9(1), 305-313

R.YIF.62
14:00
Authors : Jéssica D. C. Santos1, Cláudia Nunes1, Paula Ferreira2, Manuel A. Coimbra3, Idalina Gonçalves1
Affiliations : 1- CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal 2- CICECO-Aveiro Institute of Materials, Department of Materials and Ceramic Engineering, University of Aveiro, 3810-193 Aveiro, Portugal 3- LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal

Resume : Many plastic materials offer an opportunity to decrease weight, while enhancing the efficiency of their products. Hence, the development of lightweight fillers for plastic formulations is on demand. In this work, starch was used to develop lightweight calcium carbonate-based fillers for low density polyethylene (LDPE) materials. Under a circular economy concept, starch and calcium carbonate (CaCO3) were recovered potato washing slurries and eggshells, respectively. Commercially available CaCO3 was used as reference. The influence of these fillers on optical, physicochemical, and mechanical properties of LDPE formulations was studied. CaCO3 recovered from eggshells shows a similar size (ca. 10 µm) than the commercial one (ca. 8 µm) and is 3% less dense. On the other hand, CaCO3/starch-based conjugates have higher particle sizes (ca. 60 µm) and are 15% lighter than the reference. When incorporated into LDPE formulations, CaCO3/starch-based fillers conferred a yellowish coloration to the LDPE materials, that may be related with the starch sensitivity to the temperature used on LDPE/fillers melt-mixing. When compared with the plastic produced using commercially available CaCO3, the CaCO3/starch-based fillers decrease in 5% the density of LDPE materials and increase their mechanical rigidity, which may be potentiated by the hydrogen bonding between starch and the LDPE network. Therefore, CaCO3/starch-based conjugates can be considered to develop lightweight LDPE materials. Acknowledgments: Thanks are due to the University of Aveiro and FCT/MCTES for the financial support of CICECO (FCT Ref. UID/CTM/50011/2019) and QOPNA (FCT UID/QUI/00062/2019) through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement, and to the Portuguese NMR Network. FCT is also thanked for the Investigator FCT program (PF) and to the Scientific Employment Stimulus program (IG). The authors acknowledge to PLASTICOLIGHT (POCI-01-0247-FEDER-33848), financed by FEDER through POCI, to ?Isolago ? Indústria de Plásticos, S. A.? (PLASTICOLIGHT leader); and to ?Derovo? and ?A Saloinha, Lda.? for providing the wastes.

R.YIF.63
14:00
Authors : Caio H. N. Barros, Henry Devlin, Dishon W. Hiebner, Stephanie Fulaz, Stefania Vitale, Laura Quinn, Eoin Casey
Affiliations : School of Chemical and Bioprocess Engineering, University College Dublin, Ireland

Resume : Curcumin, the active ingredient of turmeric (Curcuma longa), is very well known for its wide range of biological activities. Amongst them, its antibacterial and antibiofilm capacity are well-known, however hindered by its low water solubility and thus low delivery efficiency to the desired site. This work presents a valuable method for increasing curcumin?s antibiofilm activity via conjugation with silica nanoparticles. Specifically, Pseudomonas putida was used to produce model biofilms to assess the diffusion, delivery and antimicrobial/antibiofilm efficacy of the curcumin-functionalised nanoparticles (curc-NPs). Biofilms are known for their increased resistance against antimicrobials due to the existence of extracellular polymeric substances (EPS) and therefore represent an extra hurdle for curcumin delivery. The curc-NPs were shown to be stable both sterically and electrostatically and were more effective than free curcumin at the same concentration at disrupting pre-formed biofilms and impeding the proper development of P. putida biofilms. The bacterial cell viability of the biofilms was also significantly decreased. Furthermore, a proteomics study of extracted EPS matrices of biofilms grown in the presence of curc-NPs was carried out, and mechanistic insights of the action of this nanoparticle were acquired.

R.YIF.64
14:00
Authors : Stephanie Fulaz, Henry Devlin, Dishon Hiebner, Caio H.N. Barros, Stefania Vitale, Laura Quinn, Eoin Casey
Affiliations : UCD School of Chemical and Bioprocess Engineering, University College Dublin, Ireland

Resume : The formation of biofilms, bacterial communities enclosed in a self-produced matrix, raise the challenge to fight infections because the existence of the extracellular polymeric substances (EPS) largely contributes to the decrease of antimicrobial susceptibility. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most frequently occurring infections, whose treatment is mainly based on vancomycin (VAN) and daptomycin, with emerging susceptibility issues. Given the long timeline for novel drug development, repurposing existing drugs and improving antibiotic efficacy should be considered. Amongst the antibiofilm strategies currently being tested, nanoparticles (NPs) are showing promise as antimicrobial carriers. Here, mesoporous silica nanoparticles (MSNs) of different surface functionalization (bare-B, amine-D, carboxyl-C, aromatic-A) were investigated for VAN encapsulation and delivery, its entrapment in the EPS matrix and resulting decrease in cell viability of two clinical isolates of S. aureus, MSSA and MRSA. Negatively charged MSN-B and MSN-C encapsulated a higher concentration of VAN in comparison to positively charged MSN-D and MSN-A. All NPs tested effectively reduced cell viability, with a 3-6-fold decrease in viable cells for MRSA compared to free VAN. MSNs improved drug penetration and protected VAN from deactivation in the EPS matrix. Thus, allowing the use of lower VAN concentrations, while increasing its local concentration delivered to the cells.

R.YIF.65
14:00
Authors : Rotar, D.V. (1), Kobasa, I.M. (2), Kornyakov A. O. (3), Vorobets, M.M. (4)
Affiliations : (1) PhD, Departments of Microbiology and Virology Bukovina State Medical University, 2, Theater Square, Chernivtsi, 58002, Ukraine E-mail: diana.rotar@bsmu.edu.ua (2)Yu. Fedkovych Chernivtsi National University. , Ukraine Kotsiubynskiy str 2 Chernivtsi 58012 E-mail: i.kobasa@chnu.edu.ua (3) Bukovyna State Medical University, Ukraine 3rd year student of medical faculty Bukovina State Medical University, 2, Theater Square, Chernivtsi, 58002, Ukraine Email: sahosome@gmail.com (4)Yu. Fedkovych Chernivtsi National University., Ukraine Kotsiubynskiy str 2 Chernivtsi 58012 E-mail: m.vorobets@gmail.com

Resume : According to the US National Institutes of Health, over 60% of all human microbial infections are caused by biofilms. Today, biofilm formation by hospital strains by bacteria is a serious threat to practical health care. We conducted a study aimed at the effect of nano-materials on the effect of film formation by reference and clinical strains of microorganisms. Screening studies were conducted to detect changes in the effect of film formation on objects which coated nano-TiO 2 and basalt tuff. With fingerprint steklyshek control samples coated with acrylic paint only basalt and tufa culture S . aureus ATCC 25923 was seeded from 1.31 × 10^2 CFU / cm ^2 to> 3.0 × 10 ^2 CFU / cm^ 2 , the concentration of bacterial cells in the extracted fluid from the surface of the glasses or remained as in the working suspension of 10 ^5 cells / ml , or increased to 10 ^9 cells / ml. As for E . coli ATCC 25922 , the data samples from the surface of vysivalys 1,22 × 10^ 2 CFU / cm ^2 to> 3,0 × 10^ 2 CFU / cm ^2 , and the concentration of viable microorganisms in the liquid from the surface ranged from 10 ^6 cells / mL, or grew to> 10 ^9 cells / ml. The next step in establishing the effect of nano-TIO 2 on the adhesive and colonization properties of the reference cultures was demonstrated. Addition to the roofing material of nano-TIO 2 from 0.05% to 2% (samples No. 6-No. 9) significantly changed the adhesive properties, so, from the surfaces of these samples sown 1.14 × 10^2 CFU / cm 2 to 0.6 × 10 ^1 CFU / cm 2 , respectively, on E . coli ATCC 25922 , from surface samples from ?6-9 0,98 × 10^2 CFU / cm^2 to 0,22 × 10^2 CFU / cm^2 . The experiment found that nano-based materials affect the ability of microorganisms to form biofilms , which can be used in medical practice.

R.YIF.66
14:00
Authors : S.Yu. Turishchev1, S.S. Antipov123, E.V. Parinova1, N.V. Praslova1, O.A. Chuvenkova1, E.A. Belikov1, E.V. Preobrazhenskaya3, D.A. Koyuda1, V. Sivakov4, O.N. Ozoline3
Affiliations : 1Voronezh State University, Voronezh, Russia, 2Immanuel Kant Baltic Federal University, Kaliningrad, Russia, 3Institute of Cell Biophysics, Pushchino, Russia, 4Leibniz Institute of Photonic Technology, Jena, Germany

Resume : Dps protein of Escherichia coli (E.coli) belongs to the ferritine-like group and represents a nanoscale hybrid particles consisting of 9 nm organic shell and 5 nm inorganic core. The protein shell is formed by a twelve identical subunits with the known structure as a dodecamer. In present paper the direct experimental information about specificity of iron atoms local surrounding in ferritines immobilized into planar and nanostructured silicon surfaces using a soft X-ray synchrotron radiation spectroscopy have been applied. Additionally, high resolution cryo-transmission electron microscopy, AFM, dynamic light scattering have been performed. The thermal and ion beam treatments were used for surface modification. The presence of both Fe2+ and Fe3+ ions in the octahedral and tetrahedral surrounding of O atoms in the Dps protein samples consisted of ~10 nm hybrid particles with ~5 nm inorganic cores were observed. Partial Fe restoration followed by the surface post treatment is shown revealing a complex composition of the hybrid particles cores even in the native Dps protein, that has been isolated from aerobically grown bacteria. These proteins containing inorganic iron-oxygen nanoparticles can be considered perspective for a novel low-cost and energy effective technology for the functional low-dimensional materials formation.

R.YIF.67
14:00
Authors : Jéssica D. C. Santos1, Cláudia Nunes1, Kamila Kapu?niak2, Janusz Kapu?niak2, Paula Ferreira3, Manuel A. Coimbra4, Idalina Gonçalves1
Affiliations : 1CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal 2Faculty of Science and Technology, Jan Dlugosz University in Czestochowa, 42-200 Czestochowa, Poland 3CICECO-Aveiro Institute of Materials, Department of Materials and Ceramic Engineering, University of Aveiro, 3810-193 Aveiro, Portugal 4QOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal Presenting author: jessica.santos2604@ua.pt

Resume : The gelling ability of starch has been explored in organic/inorganic formulations to develop porous materials [1]. On the other hand, the inherent high density of calcium carbonate (CaCO3), common filler in plastic industry, limits its application for the development of lightweight plastics. To overcome this drawback, in this work, the influence of gelatinize eggshell powder/starch conjugates assisted by microwave irradiation on optical, morphological, and physicochemical properties was studied. For comparison, a conventional gelatinization process and commercial CaCO3 were used. Starch was recovered from potato washing slurries and eggshells powders were used as the CaCO3 matrix. When assisted to microwave irradiation, gelatinized eggshell powder/starch conjugates show a whiteness level, chemical profile and crystalline structure comparable with the commercial CaCO3. These conjugates have higher particle size and specific surface area than the commercial CaCO3. However, their density is 24% lower than the reference. By gelatinizing eggshell powder/starch conjugates through conventional heating only 18% of density is decreased. Therefore, the microwave irradiation seems to potentiate the density decrease of eggshell powder/starch, offering a new strategy to develop lightweight fillers for plastic formulations. Acknowledgments: Thanks are due to the University of Aveiro and FCT/MCTES for the financial support of CICECO (FCT Ref. UID/CTM/50011/2019) and QOPNA (FCT UID/QUI/00062/2019) through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement, and to the Portuguese NMR Network. FCT is also thanked for the Investigator FCT program (PF) and to the Scientific Employment Stimulus program (IG). The authors acknowledge to PLASTICOLIGHT (POCI-01-0247-FEDER-33848), financed by FEDER through POCI, to ?Isolago ? Indústria de Plásticos, S. A.? (PLASTICOLIGHT leader); and to ?Derovo? and ?A Saloinha, Lda.? for providing the wastes. References: [1] A. F. Lemos and J. M. F. Ferreira, Mater. Sci. engeering, 11 (2000)

R.YIF.68
14:00
Authors : Silambarasan Anbumani * (1), Aldeliane M. da Silva (1), A.A.G. von Zuben (1), Carlos L. Cesar (1), Alessandra A. de Souza (2), Monica A. Cotta (1)
Affiliations : (1) Institute of Physics ?Gleb Wataghin?, University of Campinas-13083-859 Campinas, São Paulo, Brazil; (2) Citrus Center APTA ?Sylvio Moreira? Agronomic Institute of Campinas 13490-970 Cordeirópolis, São Paulo, Brazil

Resume : SU-8 is an epoxy-based photoresist which has been used as a novel platform for biomedical applications due to its chemically tunable and biocompatible surface in addition to its relatively high stiffness, chemical resistance, optical transparency and ease of processing properties. In this work, we tailor SU-8 surface properties to investigate single cell motility and adhesion of the bacteria Xylella fastidiosa. Different SU-8 samples have been prepared using UV illumination, thermal processing, and oxygen plasma treatment. Atomic Force Microscopy and X-Ray Photoelectron Spectroscopy were used to determine nanoscale surface properties; ex-vivo studies at the level from single cell to biofilm formation were carried out with Confocal Laser Scanning Microscopy (CLSM). The mean velocity and displacement of single cells have been extracted from CLSM tracking information data and the size and quantity of biofilms are compared for different samples. We observed a significant difference in bacterial cell motility, adhesion and also biofilm architecture on SU-8 as nanoscale surface property changes. Larger density of carboxyl groups in SU-8 surfaces provide enhanced cell motility, while denser biofilms are found in untreated SU-8. Our results can improve understanding of the role of nanoscale properties on bacteria-surface interaction and thereby create strategies to preventing microbial adhesion and consequently, biofilm development of pathogenic species.

R.YIF.69
14:00
Authors : M. Shkunov(1), A.N. Solodukhin(2), P. Giannakou(1), D.O. Balakirev(2), Y.N. Luponosov(2), S.A. Ponomarenko(2,3)
Affiliations : (1) Electrical and Electronic Engineering, Advanced Technology Institute, University of Surrey, Guildford, UK; (2) Enikolopov Institute of Synthetic Polymeric Materials of the Russian Academy of Sciences; (3) Chemistry Department, Lomonosov Moscow State University, Russia

Resume : Opto-stimulation of semiconductor-biointerfaces provides efficient pathways towards eliciting neural activity through selective spectral excitation. In visual prosthesis, tri-colour excitation capability, is the key to restoring full-colour vision. Among organic semiconductor materials, donor?acceptor molecules have the most promising properties due to excellent light absorption, high stability and good solubility in organic solvents. Varying the donor and acceptor functionalities allows fine tuning of their absorption bands. These carbon-based molecules bear intrinsic affinity to biological systems, demonstrate excellent photoresponses, electronic and ionic conductivity, and allow intimate interface with liquid bioenvironment. We demonstrate tri-colour optoelectronic devices based on solution-processable conjugated donor-acceptor molecules with absorption in red, green and blue spectral regions, mimicking the absorption of human retinal cones. Photo-response is studied via interfacing with biological electrolyte solution and using long-pulse, narrow-band excitations, where both photo-voltage and photo-current responses show clear signatures of capacitive charging at the electrolyte/device interface. We further explore ink-jet printing to reproduce individual photo-pixels with red, green and blue absorption characteristics, with small diameters of 35 to 45 microns. We discuss the suitability of organic semiconductors for potential medical applications as retinal bio-engineered prosthesis for the restoration of human vision, and evaluate prospects of wider, colour-selective excitations of bio-interfaces. (This work was supported by Russian Science Foundation grant 19-73-30028).

R.YIF.70
14:00
Authors : Ana Arché-Núñez [1], Beatriz L. Rodilla [1,2], Ana Domínguez-Bajo [3], Ankor González-Mayorga [4], Ivo Calaresu [5], Rossana Rauti [5], Denis Scaini [5], Julio Camarero [1,6,] Rodolfo Miranda [1,6], Elisa López-Dolado [4], Laura Ballerini [5], Lucas Pérez [1,2], M. Concepción Serrano [3] and M. Teresa González [1]
Affiliations : [1] Instituto Madrileño de Estudios Avanzados (IMDEA Nanociencia), 28049, Madrid, Spain; [2] Depto. Física de Materiales. Universidad Complutense de Madrid (UCM), 28040, Madrid, Spain; [3] Instituto de Ciencia de Materiales de Madrid (ICMM-CSIC), 28049 Madrid, Spain; [4] Hospital Nacional de Parapléjicos, 45071 Toledo, Spain; [5] International School for Advanced Studies (SISSA) 34136 Trieste, Italy; [6] Depto. Física de la Materia Condensada-IFIMAC. Universidad Autónoma de Madrid, 28049, Madrid, Spain

Resume : Neural activity can be measured or stimulated using electrodes that can be improved with nanotechnology advances. In this work, we propose the use of template-assisted electrodeposition technique to obtain, in a reproducible way, nanostructured metallic surfaces that can work as neural electrodes. On one hand, planar electrodes are used in extracellular experiments and medical applications to register or stimulate neural activity. In the context of the ByAxon project, our results on the fabrication of low-disturbance flexible nanostructured planar electrodes based on vertical metallic nanowires standing over a flexible Au base will be presented. We have obtained low-impedance electrodes by electrodeposition using anodic aluminium oxide templates fabricated at the laboratory, controlling the geometry of the electrode?s nanostructured surface. Biocompatibility studies with these electrodes showed proper neural cell adhesion, growth and differentiation. On the other hand, to perform intracellular in vitro recordings, patch-clamp technique is the most extended one. However, it damages the cell due to perforation size and medium exchange reducing life time when measuring. In this work, our progress in the fabrication of sharp nanostructured metallic electrodes based on the process described above, in order to improve the existing intracellular recordings, will be shown. Work funded by H2020 R&D programme under grant agreement No. 737116 and I D I project DPI2017-90058-R

R.YIF.71
14:00
Authors : H.Gogotsi(a),,O.Ivanyuta(a),,E.Buzaneva(a),U.Ritter(b),P.Scharff(b)
Affiliations : (a)National Taras Shevchenko University of Kyiv,, 64, Volodymirska Str., 01601 Kyiv, Ukraine, oivanuta@gmail.com (b)TU Ilmenau, Institut für Chemie and Biotechnology, Postfach 100565, 98684 Ilmenau, Germany.

Resume : The design and optical characterization of the protein amino - acids is key for understanding of the amino-acids molecules behaviour as basis sequences of biological macromolecules ( proteins) and their using as photoactive molecules, for example, in an advanced photovoltaic systems for an energy sources [1]. Therefore, we have start to design amino-acids molecules and study classic models for light excited electron transition through LUMO-HOMO band gap for the molecules and discuss ones using theories for LUMO-HOMO band gap formation for these molecules having different confirmations [2]. Then we selected amino-acid molecule for studies by the PL spectroscopy take in account that can use the laser with 408 nm wavelength (3.3 eV) for experiments. The cysteine PL excited by the laser with wavelength 408 nm( 3.3 eV) is characterized by the band in 1.5- 2.7 eV range. This band included of subbands, which are centered at 2.04, 2.24 and 2.4 eV. The present of subbands are explained by the dependence on the conformation of the molecule geometry in the cysteine layer of the HOMO-LUMO value as it is found due to compare of these subbands with calculations of electronic excitations of the cysteine conformers [2]. Therefore it is possible to use light excited electron transitions in the cysteine layer with different molecule geometry in photonic systems. 1. Koo, Suk Tai Chang, Joseph M. Slocik, Rajesh R. Naik and Orlin D. Velev, Aqueous soft matter based photovoltaic devices, J. Mater. Chem., 2011, Advanced Article.http://pubs.rsc.org/en/content/ArticleLanding/2011/JM/COJMO1820A#divAbstract;2.R. Maul, M. Preuss, F. Ortmann, K. Hannewald, and F. Bechstedt, Electronic Excitations of Glycine, Alanine, and Cysteine Conformers from First-Principles Calculations, J. Phys. Chem. A 2007, 111, p.4370-4377.

R.YIF.72
14:00
Authors : Francesca Guagnini, Peter B. Crowley
Affiliations : School of Chemistry, National University of Ireland, Galway, Ireland

Resume : Thanks to their versatility, proteins are ideal building blocks for functional materials. Controlled protein assembly is necessary for bottom-up fabrication of biomaterials.[1] Many strategies can be employed to achieve protein assembly, like computational design of protein interfaces, supercharged proteins or tethered linkers.[1] The use of macrocyclic receptors is a viable alternative. Macrocyclic receptors, like cucurbiturils or calixarenes, recognize patches on protein surfaces and induce assembly / crystallization by bridging two or more proteins. This transferable approach has been demonstrated in different proteins and does not require expensive protein functionalization or the use of designed proteins.[2-3] Metal coordination is another directional interaction employed to facilitate protein assembly.[4] We postulated that macrocycle recognition coupled with metal coordination will increase the control over assembly design. To demonstrate this idea, we tested two different protein model systems: a β-propeller lectin and a highly cationic small protein. Our research paves the way for new biohybrid materials. [1] Luo, Q.; Hou, C.; Bai, Y.; Wang, R.; Liu, J., Chem. Rev., 2016, 116, 13571–13632. [2] Rennie, M. L.; Fox, G. C.; Pérez, J.; Crowley, P. B., IUCRJ, 2019, 6, 238-247. [3] Guagnini, F.; Antonik, P. M.; Rennie, M. L.; O’Byrne, P.; Khan, A. R.; Pinalli, R.; Dalcanale, E.; Crowley, P. B., Angew. Chemie Int. Ed., 2018, 57, 7126–7130. [4] Bailey, J. B.; Zhang, L.; Chiong, J. A.; Ahn, S.; Tezcan, F. A., J. Am. Chem. Soc., 2017, 139, 8160–8166.

R.YIF.73
14:00
Authors : V. Dinca1, L. E. Sima2, M. Icriverzi2,3, M. Uta2, N. Nichita-Branza2, A. Roseanu2, L. Rusen1, A. Bonciu1,4, E. C. Sirigim5,6, P. Hoffmann5 and M. Dinescu1
Affiliations : Affiliations : 1National Institute for Lasers, Plasma and Radiation Physics, Bucharest, Romania 2Institute of Biochemistry of the Romanian Academy IBAR, Bucharest, Romania 3University of Bucharest, Faculty of Biology, Bucharest, Romania 4University of Bucharest, Faculty of Physics, Bucharest, Romania 5EMPA, Switzerland 6ETH, Switzerland

Resume : Micro and nano-biointerface has been emerging as a key topic for tissue regeneration, basic biology sciences, and medicine in recent years. Understanding the influence of specific characteristics of materials at biological interfaces can bring advantages to designing two and three-dimensional biointerfaces able to guide cell fate in a controllable and accurate way. By an appropriate combination of fabrication technologies and particular materials properties, effective design tailored supports and scaffolds with controlled micro nanoscale topography and chemistry can be achieved towards controlled cellular microenvironments as a powerful tool for fundamental biology studies. Within this context, in this work, laser processing of micro-structured biomaterials ( i.e ceramics, polymers, PDMS replica starting from laser processed surfaces) with convex and concave topographies for evaluating the role of materials surface topography on different mammalian cell lines responses are presented. For example, laser direct texturing and Matrix-Assisted Pulsed Laser Evaporation -MAPLE were used for modulating specific characteristics of biointerfaces, which roles were studied by investigating the biocompatibility and biological reactions induced by new materials, especially the immune response and the capacity for bone regeneration and hepatocyte-like cells differentiation. The physicochemical characteristics of the microstructured surfaces, along with the in vitro analyses, suggest that our designed interfaces represent an efficient way to tackle the design of implant surfaces or smart interfaces for a wide range of biomedical applications.

R.YIF.74
14:00
Authors : Rouhollah Jalili, Dorna Esrafilzadeh,Rose Amal
Affiliations : Rouhollah Jalili: School of Chemical Engineering, University of New South Wales (UNSW), Sydney, NSW 2052, Australia; Dorna Esrafilzadeh: Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW, 2031 Australia; Rose Amal: School of Chemical Engineering, University of New South Wales (UNSW), Sydney, NSW 2052, Australia;

Resume : Additive manufacturing is also known as 3D printing, is enabling the production of structured functional materials and has the potential to close the gap between discoveries and manufacturing by providing an effective path toward favorable device cost and performance. Central to many promises of 3D printing is to overcome the limitations of conventional device productions to allow freedom of design and provide new functionality while substantially lower the materials cost and energy. Although, additive manufacturing is coined as the revolutionary manufacturing technique of choice, by mimicking nature?s pathway in creating functional systems, perhaps the most important neglected aspect which severely overshadows its promises is the lack of a platform to bridge the gap between the nano-world to the macro-world, which nature elegantly executes. This calls for the convergence of typical 3D printing techniques with a resolution at the molecular level to effectively exploit the nanoscale properties of individual building blocks in the final architecture. If this achieved, 3D printing can be effectively used to print complex multifunctional electrodes containing both scaffolding and active materials for batteries, supercapacitors and electrocatalysis applications. Biological systems implement anisotropic molecular building blocks and their remarkable self-assembling properties to achieve liquid crystal mediated complex structural arrangements and patterns. Following this model, we use self-assembly to impart the fine-tuning required for the design-freedom of architecturally complex systems at the nanoscale with intricate patterns within 3D printed constructs. The printing ink prepared from a mixture of liquid crystals (LC) of nanocellulose (NC) whiskers and large sheets of graphene oxide (GO), which shows highly ordered laminar organization not inherently present in the source materials. The bioinspired 3D printing approach exemplifies how the self-assembly approach in the traditional 3D printing processes can unlock the potential of individual constituent phases to print macroscopic architectures with nanoscopic arrangements. Our demonstrations will serve as a roadmap in additive manufacturing technologies for extensive development ranging from the choice of materials in nanoscale, design, and construct to device performance.

R.YIF.75
14:00
Authors : Aditi Pandey1, Nai-Chia Teng2, Jen-Chang Yang1
Affiliations : 1Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11052, Taiwan, ROC. 2 School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 11052, Taiwan, ROC.

Resume : Dental caries, a critical issue faced by children in poor communities, can credibly be arrested by silver diamine fluoride (SDF), a popular cariostatic agent, though leaving behind black stains on the teeth. To rectify this issue, current work aims to develop polyelectrolyte-based (involving electrostatic interactions with positive silver ions) formulation, comprising of nano silver fluoride (PE-NSF). UV-Vis spectroscopy was used to characterize the PE-NSF, depicting formation of silver nanoparticles by a distinct peak. Further, the particle size and microstructure were analyzed by DLS and TEM. Carious primary molars were utilized for the extraction of dentin specimen and sorted based on tooth size and category, to which SDF and PE-NSF were applied for assessing the staining, until a period of 7 days. Teeth in the PE-NSF were unstained while SDF group were black-stained after 7 days incubation time. Moreover, the arrested caries by PE-NSF were also comparable to that of SDF. The morphological and elemental investigations of the teeth samples were examined by SEM, EDX, and TEM for study of presence of collagen in the arrested lesions and remineralised crystalline apatites. Therefore, the PE-NSF can serve as a potential candidate for the dental caries treatment, without staining the teeth.

R.YIF.76
17:45 Ceremony Handing in The E-MRS Certificates on The BEST Presentation: Professor Insung S.Choi with The Young Investigator Forum Organizers, GENERAL PHOTO    
 
GENERAL POSTER SESSION : Chairs - Insung S. Choi (Korea), Peter Scharff (Germany), Emmanuel Stratakis (Greece), Thomas J. Webster (USA), Eugenia Buzaneva and Oleksandr Ivanyuta (Ukraine) - (18:00 - 18:30)
18:00
Authors : R.M. Dodo1, M. Abdulwahab1,2, U. Shehu1, F. Asuke1, A. Hamisu1
Affiliations : 1Department of Metallurgical and Materials Engineering, Ahmadu Bello University, Zaria, Nigeria 2Department of Chemical, Metallurgical and Materials Engineering, Tshwane University of Technology, Pretoria, South Africa

Resume : The present study explored wear and biocompatibility characteristics of polypropylene (PP)/ periwinkle shell particulates (PWS) composites for dental implant application. Wet-sliding the stainless steel ball against specimen disc revealed the abrasive wear behaviour of the composites under load and linear speed of 5N and 10 cm/s respectively. In the wear tests, the specimens were wetted with simulated human saliva with and without sodium fluoride (NaF) additive. On the other hand, a mouse specimen was used to carry out a biocompatibility test. The composite demonstrated superb wear resistance at 40 and 50wt% reinforcement loading. Further, results showed that wear rate decreased considerably with increasing PWS content. Higher wear rate was noted on composites under condition of simulated human saliva with NaF addition. The biocompatibility test reveals that developed composite is harmless to the living tissues. Thus, PP/PWS composites could be recommended to be used as dental restorative material.

R.P.1
18:00
Authors : Toshifumi Imajo, Takashi Suemasu, Kaoru Toko
Affiliations : Univ. of Tukuba

Resume : To realize high-performance thin-film transistors, solid-phase crystallization (SPC) of Ge films on insulators has been widely investigated, which is a simple method to directly form polycrystalline thin films on insulators. Recently, we succeeded in increasing the grain size of the Ge thin film on the glass substrate by controlling the density of amorphous Ge, which is the precursor of SPC, and have continued to update the highest record of mobility of the low-temperature synthetic film [1,2]. In this study, we develop this SPC-method onto plastic substrate. In the experiment, GeO2 underlayer was sputtered on both SiO2 glass and plastic substrates. After that, amorphous Ge precursors were prepared by molecular beam while heating the samples at 150 °C. Finally, the samples were then loaded into a conventional tube furnace in a N2 atmosphere and annealed (375 °C, 150 h) to induce SPC. As a result, SPC-Ge formed on a plastic has almost the same crystal grain size as that on a glass. By examining the electrical properties of substrate dependence of Ge layer, we found that there is almost the same tendency of electrical properties between the samples on plastic and glass substrates. With the above, we performed post annealing (500 °C, 5 h) in order to reduce the grain boundary barrier and the impurity scattering, and achieving hole mobility of 680 cm2/Vs for Ge thin film on plastic substrate. It is the highest value to date among those of semiconductor layers directly formed on insulator and is a result for exploiting excellent flexible devices. [1] K. Toko et al., Sci. Rep. 7, 16981 (2017). [2] T. Imajo et al., Applied Physics Express 12, 015508 (2019).

R.P.2
18:00
Authors : O. Ivanyuta
Affiliations : Department Electrophysics, Faculty of Radiophysics,Electronics and Computer Systems, Taras Shevchenko Kiev National University, Volodymyrska str. 64, 01033 Kiev, Ukraine, iva@univ.net.ua

Resume : Interest in metal nanostructures stems from their unique optical properties related with single-particle or collective plasmons. The aim is focused on optical properties of gold nanorods and silica/gold nanoshells, whereas the synthesis protocols and biomedical applications are discussed shortly. The effects of the gold nanoshells structural polydispersity and the surface electron scattering in a thin metal layer on the resonance light scattering spectra are studied theoretically and experimentally for the silica/gold nanoshell water colloids. Both the gold nanorods and nanoshells are exceptionally biocompatible nanomaterials, which surface can be easily functionalized by key probe molecules such as antibodies, oligonucleotides, biotin. We provide an illustration of erythrocyte and living bacteria imaging with silica-gold nanoshells as resonance-scattering labels. In this case, nanoshells are seen as bright red dots against a dark background, except for yellow areas of aggregation, where nanoshells are in close proximity and the plasmon resonance wavelength is changed.

R.P.3
18:00
Authors : Pengxiang Si, Fan Jiang, Qingsha S. Cheng*, Geoffrey Rivers, and Boxin Zhao*
Affiliations : P. Si, Dr. G. Rivers, Prof. B. Zhao Department of Chemical Engineering Waterloo Institute for Nanotechnology University of Waterloo 200 University Avenue West, N2L 3G1, Canada F. Jiang, Prof. Q. S. Cheng Department of Electrical and Electronic Engineering Southern University of Science and Technology 1088 Xueyuan Ave, 518055, China

Resume : Antennas are an essential component of next generation wearable electronics for wireless communication. It is highly desirable to develop a robust self-healable antenna to maintain the integrity and function of the device because the wearable devices are becoming ever-thinner and smaller, making them subject to serious wear and damage during the body bending or stretching. However, it has been a significant material challenge as the integral antenna can not be exposed to external stimulus such as heat, light and chemical agents without damaging the devices. Herein, we design a latex-polyelectrolyte coacervation (LPC) system compounded with conductive filler to form a printable conductive paste which exhibits robust toughness, stiffness, extensibility, high electrically conductivity, one-step printability and self-healing capability simultaneously. The formation of polymer network via electrostatic interaction and hydrophobic interaction within antenna provide a self-healing mechanism via the absorption of moisture in the ambient air. The LPC system is promising for broad applications in self-healable stretchable electronics.

R.P.4
18:00
Authors : Oleksandr Ivanyuta1, Eugenia Buzaneva2, Uwe Ritter3, Peter Scharff3
Affiliations : 1 Faculty of Radiophysics Electronics and Computer Systems, Taras Shevchenko National University of Kyiv, Ukraine E-mail: iva@net.univ.ua 2 The Scientific and Training Center on Physical and Chemical Material Science, National Taras Shevchenko National University of Kyiv and NAS of Ukraine 3 Institute of Chemistry & Biotechnology, T U Ilmenau, Germany

Resume : Biofunctionalization of carbon nanotubes, which are well-known for their unique complex of properties, is in the centre of research attention for numerous biomedical applications, also as for fundamental study of nanotubes as templates. Covalent or non-covalent immobilization of biomolecules on carbon nanotubes opens the doors for novel biocomposite manufacturing and biosensing. Carbon nanotube functionalization by DNA, RNA, oligonucleotides or single nucleotides is of special interest as possible approach for nucleic acid sensing, gene delivery, gene therapy, clinical diagnosis and pathogen monitoring. It is also used in nanotechology as technique for suspensioning, characterization and separation of nanotubes. In our investigations the non-covalent immobilization in aqueous suspensions of AMP and DNA on multi-walled carbon nanotubes developed. Previous oxidation of multi-walled carbon nanotubes by ozonolysis were used if it was necessary. The characterization of functionalized nanotubes was carried out by Scanning Electron Microscopy, Transmission Electron Microscopy, Optical Microscopy, Raman, UV-visible-NIR, IR- and Photoluminescence Spectroscopies. Various models of interface formation between biomolecules and nanotube surface are discussed. Optical characteristics of novel biofunctionalized nanotubes also gave us necessary information for their future application as biosensors or activating agents.

R.P.5
18:00
Authors : Monica Marini 1, Bruno Torre 2, Marco Allione 2, Maria Caterina Morello 2, Manola Moretti 2, Andrea Giugni 2, Enzo di Fabrizio 2
Affiliations : 1 DISAT, Politecnico di Torino, Corso Duca Degli Abruzzi, 24, 10129 Torino, Italy. monica.marini@polito.it 2 SMILEs Lab, PSE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia

Resume : Oriented and self-organized DNA filaments were obtained by micro-fabricated super-hydrophobic surfaces (SHS). The bio-macromolecule, suspended over the SHS, can be characterized by using several techniques, such as electron microscopy and optical spectroscopy. DNA direct imaging by high resolution TEM (HRTEM) allowed solving the base pairs with a resolution of 1.5 Å [1,2] and the metrological details can be effectively corroborated by Raman spectroscopy data [3,4]. Raman spectra in the range 600-1800 cm-1 were acquired on suspended DNA filaments and on the droplet residual. The spectra obtained at the same working conditions on DNA samples and buffer deposited over a CaF2 window were used as negative control. The study of the spectra revealed the absence of physiologically compatible buffers on suspended filaments while their contribution is strong in the DNA spectra acquired on CaF2 windows and on the droplet residual. This suggests that the optimized SHS platform separates small molecules from the suspended DNA and the non-interacted material is concentrated in the droplet residual. The SHS-DNA platform revealed a strong potential for the study of polarized Raman spectra as the DNA filaments are autonomously oriented with different angles over the device and for the analysis of the presence and influence of molecules affecting the DNA double helix such as chemotherapeutic compound (Cisplatin), heavy metals and methylations.

R.P.6
18:00
Authors : Dr., Docent Oleksandr .Ivanyuta
Affiliations : Taras Shevchenko National University of Kyiv,64/13, Volodymyrska Str., Kyiv, 01601, Ukraine iva@univ.net..ua

Resume : The ds-DNA supramolecules encodes the architecture and function of cells in all living organisms. A theoretical model for the temperature dependence of the electrical conductivity of DNA is presented, based on microscopic models and percolation theoretical arguments. The theoretical results, excluding or including correlation effects, are applied to recent experimental findings for DNA, considering it as a one-dimensional molecular wire. Two strands may come together through hydrogen bonding of the bases A with T(A T) and G with C (GC). The obtained current-voltage curves were compared with the approximated ones for semiconductor, dielectric, and conductor. Its resistance was greater than 1014 Ω in the region of low bias. The enlightenment of this issue could improve long range chemistry of oxidative DNA damage and repair processes, monitoring protein-DNA interactions and possible applications in nano-electronic circuit technology. A smaller current gap was found showing that the electrical conductivity can be somewhat controlled by silver and gold deposition. The ds-DNA template is flexible enough that it can be shaped in many different way’s geometrical structures of DNA. Periodic arrays are being examined as the main components in nanoscale memory devices and other electronic applications. The approach to build functional devices has relied on the modification of semiconductor surfaces with DNA. These modified surfaces can then be used to fabricate nanoscale electronic devices on a silicon with carbon monolayer to attach double-stranded DNA. The study of electrical conductivity has crucial importance in the development of biosensors.

R.P.7
18:00
Authors : Alexina Ollier1,2, Marcin Kisiel1, Urs Gysin1 and Ernst Meyer1
Affiliations : 1 Institute of Physics, University of Basel, Switzerland 2 Swiss Nanoscience Institute, Klingelbergstrasse 82, 4056 Basel, Switzerland

Resume : Understanding nanoscale energy dissipation is nowadays among few priorities particularly in solid state systems. Breakdown of topological protection, loss of quantum information and disorder–assisted hot electrons scattering in graphene are just few examples of systems, where the presence of energy dissipation has a great impact on the studied object. It is therefore critical to know, how and where energy leaks. High sensitivity pendulum geometry Atomic Force Microscope (AFM), oscillating like a pendulum over the surface, is perfectly suited to measure tiny amount of dissipation. The tip position on the sample is controlled with atomic accuracy owing to a tunneling current line and the enhanced sensitivity allows to distinguish between electronic, phononic or van der Waals types of dissipation. Measurements can be performed in a wide range of temperatures from 5K to room temperature and in magnetic fields spanning from B=0T to B=7T. The design of the sample holder allows to perform dissipation measurements while passing electric current in the plane of the sample surface. In this work we performed energy dissipation measurements on a suspended graphene sheet at room temperature under UHV. The graphene is deposited on a hole patterned substrate in order to have suspended circular (with a diameter of 6.5 µm) graphene sheet. The experiments allows to investigate the phononic and electronic energy dissipation of the suspended graphene.

R.P.8
18:00
Authors : Happy Agarwal; VenkatKumar Shanmugam*
Affiliations : School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore – 632014, TN, India *Corresponding Author Mail id: happyagrawal22@gmail.com; venkatkumars@vit.ac.in

Resume : In the present study, we have biosynthesized ZnO NPs and investigated the mechanism of anti-inflammatory action of the nanoparticles in both in vitro and in vivo models. In the in vitro model system, RAW 264.7 murine macrophages were pre-incubated with ZnO NPs and the effect of NPs on the lipopolysaccharide (LPS)-induced activation of nuclear factor-kappa B (NF- κB) pathway was evaluated. Results show that LPS mediated increase in expression NF- κB pathway was downregulated by an increase in expression of inhibitor of kappa B alpha (IκBα) in NP treated cells in a dose-dependent manner. The anti-inflammatory potential of ZnO NPs was also evaluated in Carrageenan induced mice paw edema model. Administration of ZnO NPs to mice, prevented Carrageenan induced mice paw edema and also suppressed the expression of pro-inflammatory mediators like interleukin-1 beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), and cyclooxygenase-2 (COX-2). Histological analysis of mice paw tissue section clearly indicated a reduction in leukocyte and neutrophil extravasation to the tissue space in mice paw that was pre-treated with ZnO NPs. Present findings give us an insight into the ZnO NPs mediated anti-inflammatory action.

R.P.9
18:00
Authors : Soumya Menon, Dr.Venkatkumar S.
Affiliations : School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore 632014, India

Resume : Cervical cancer is the most common malignancy suffered by women worldwide in the female reproductive system. The chemotherapeutic treatment using anticancer drugs are developed recently, but they lack delivery to the targeted tumour tissues. The commonly used anticancer drug paclitaxel is highly efficient against various cancer types, but due to its low solubility the enhancement of its property decreases. Recent advances includes nanotechnology based combinatorial drug delivery systems that offer enhanced pharmacokinetic property due the EPR (enhanced permeable retention), the larger surface area to volume ratio in nanoparticles, it also helps in adsorbing large amount of drug and act as a drug carrier. The combination of nanosystems aids in active or passive drug delivery through the cell membranes via endocytosis. In particular selenium nanoparticles is a well established chemotherapeutic agent due to low toxicity to the normal tissues, when compared to other nanosystems. The selenium nanoparticles act as both prooxidant in the presence of cancer tissues by producing ROS which eventually causes oxidative stress, mitochondrial damage, alter the genetic arrangements of the cancerous tissues, they are also major component of many antioxidant enzymes that also function at protecting from tumour like GSH (glutathione), selenomethioninecystien (recent established amino acid), theoredoxin (Trx) and so on. They act as antioxidant agent for normal tissues by scavenging ROS, regulating the DNA repair proteins, and protecting the damages caused by oxidative stress. In this paper more emphasis is done on the green synthesis of selenium nanoparticles to avoid the utilization of external harmful chemicals. The precursor used for the reduction of the sodium selenite which itself it a harmful chemical which was traditionally used as anticancerous agent, are Mucuna pruriens seed extract which is also a traditional medicine against various cancer types. The extract is used as a bioreductive catalyst which also helps in stabilization of the nanoparticle development. The biosynthesized SeNPs are optimized using BB design for evaluating the optimum particle size and process variable. The optimized SeNPs are then coated with chitosan which acts a biodegradable linker against anticancer drug paclitaxel. The combinatorial nanosystem is evaluated for its anticancerious activity against HeLa cancer cells. This innovative strategy can help in overcoming the drawbacks faced by chemotherapeutic drugs with low solubility, delivery to the targeted sites, highly biodegradable and low toxicity.

R.P.10
18:00
Authors : Paulo Brites1, Gonçalo Oliveira1, Inês Vilarinho1, Cláudia Nunes1,3, Paula Ferreira2, Manuel A. Coimbra3, Idalina Gonçalves1
Affiliations : 1CICECO – Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal 2CICECO – Aveiro Institute of Materials, Department of Materials and Ceramic Engineering, University of Aveiro, 3810-193, Aveiro, Portugal 3LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal

Resume : Under the scope of circular economy, all plastic packaging on the EU market must be recyclable, biodegradable and/or compostable till 2030. On the other hand, the need to minimize agrofood waste fingerprint is also a necessity. In this work, the feasibility of using potato chips industry byproducts as a source of molecules of interest for developing biobased plastic materials was explored. From potato washing slurries, potato peels, and frying residues, starch, waxes, and oil were recovered, combined, and further converted into thermoplastic formulations through melt-mixing. The influence of waxes and oil content on relative humidity, melt tenacity, mechanical, and wettability properties of starch-based formulations was studied. Both starch/waxes and starch/oil compounds show higher humidity content than the control samples prepared using only starch. Low concentration of waxes increased in twofold the melt flow rate (MFR) of thermoplastic starch, promoting its fluidity. Contrarily, the highest waxes amount studied decreased the formulations MFR, leading to more rigid materials. With oil, an opposite behaviour was observed. When submitted to injection moulding process, both starch/waxes and starch/oil specimens showed higher elasticity and stretchability than the control samples, while maintaining their tensile strength. Moreover, the addition of waxes or oil increased the surface hydrophobicity of starch-based formulations. Therefore, potato chips byproducts can be considered a source of raw materials with potential to be used on the development of biobased plastics with improved mechanical performance and water tolerance. Acknowledgments: Thanks are due to the University of Aveiro and FCT/MCTES for the financial support of QOPNA research Unit (FCT Ref. UID/QUI/00062/2019) and of CICECO-Aveiro Institute of Materials (FCT Ref. UIDB/50011/2020 & UIDP/50011/2020). The authors acknowledge to POTATOPLASTIC project (POCI-01-0247-FEDER- 017938), financed by FEDER through POCI, “Isolago – Indústria de Plásticos, S. A.”, the project leader, “A Saloinha, Lda.” for providing potato byproducts, and to FEB – Cafés, S. A. for supplying coffee silverskin. The costs resulting from the FCT hiring is funded by national funds (OE), through FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. FCT is also thanked for the Investigator FCT program (PF, IF/00300/2015), for the Individual Call to Scientific Employment Stimulus (IG, CEECIND/00430/2017), and for the grants ref. SFRH/BPD/104712/2014 (IG), SFRH/BD/136804/2018 (JL), and SFRH/BPD/100627/2014 (CN).

R.P.11
18:00
Authors : Gerçem Altunordu, Ay?en Tezcaner, Zafer Evis, Dilek Keskin
Affiliations : Middle East Technical University; Biomaterials and Tissue Engineering Excellence Center

Resume : INTRODUCTION Bone tissue can be damaged by aging, some diseases or some accidents. Critical bone injuries and tissue losses have led to the need for the use of various biomaterials in the clinic. Firstly, metals such as cobalt-chromium alloys, stainless steel, titanium alloys have been used in biomedical applications. However, although these biomaterials support bone repair, they are not suitable for the purpose of supporting new bone formation, because they lack biological resorption characteristics. Therefore, many researchers oriented to other alternatives. One of these alternatives is the production of bioceramics such as hydroxyapatite (HA) and tricalcium phosphate. Many studies have shown that bioceramics containing silicon (Si), calcium (Ca) magnesium (Mg) have high bioactivity, biocompatibility, and strong mechanical properties. Therefore, they can be used in bone repair and regeneration as scaffolds with polymers such as polycaprolactone (PCL). The aim of study is to investigate potential of PCL/HA/Akermanite(AKR) and PCL/ carbonated hydroxyapatite (CHA)/AKR fibers produced by electrospinning method for bone tissue engineering. MATERIALS AND METHODS HA was synthesized by microwave irradiation method while CHA was synthesized by nanoemulsion technique. AKR was fabricated vis sol-gel method. After characterizations of bioceramics such as X-Ray Diffraction (XRD) and Fourier Transform Infrared (FT-IR), and bioactivity tests in simulated body fluid (SBF), scaffolds were producing by wet electrospinning. PCL using carrier material and was dissolved in ethanol. After optimization of some parameters such as voltage and flow rate, PCL/HA/AKR and PCL/CHA/AKR fibers were produced. Then, in vitro bioactivity tests of scaffolds were performed in SBF for 1,7 and 14 days to determine apatite layer formation. At the end of the immersion times, scaning electron microscopy (SEM) analysis was used for imaging apatite layers on the fibers. Also, elemental analysis (EDS) was done. Furthermore, cell adhesion, proliferation and osteogenic activity were studied with Saos-2 cells. CONCLUSION HA, CHA and AKR were synthesized respectively by microwave irradiation, nanoemulsion and sol-gel methods. Characterization techniques such as XRD and FT-IR proved that bioceramics produced successfully. In vitro bioactivity experiments showed that there are spherical apatite layer formations on the PCL/HA/AKR and PCL/CHA/AKR fibers produced by wet electrospinning. Moreover, cell proliferation and osteogenic activity of Saos-2 cells on the scaffolds were good.

R.P.12
18:00
Authors : Shigeori Takenaka
Affiliations : Director of Research center for Biomicrosensing Technology (RCBT) Professor of Kyushu Institute of Technology (Kyutech)

Resume : When fluorometric imaging of concentration change of potassium (K ) and/or sodium (Na ) ion due to channel on a cell surface is realized with molecular probe, we will understand the working of these ions as a whole in a cell and the relationship between their abnormality and specific diseases such as channelopathy. Although crown ether type fluorescence probes have been developing in this purpose, it is very difficult to do fluorometric imaging of K or Na specifically under homologues aqueous medium. Under such circumstances, we have developing new type of probe consisting guanine tetraplex (G4) forming sequence oligonucleotide with K or Na carrying two chromophore pairs of Förster resonance energy transfer (FRET), which named potassium or sodium sensing oligonucleotide, PSO or SSO, respectively [1,2]. Additional attachment of biotin with PSO realized fluorometric imaging of cellular cytoplasm K in a living cell and monitored K efflux from cell under apoptosis process [3]. As a next stage, we are tried to localize PSO on a cell surface and monotone K concentration change on a cell surface. Here, we will introduce our new approach to achieve this attempt, where the lipid-modified oligonucleotide carrying FAM (1) on a cell surface hybrids with the G4-forming oligonucleotide carrying TAMRA (2) resulting localization of PSO function on a cell surface. Here, we will discuss our approach with the related results. [1] B. Juskowiak, S. Takenaka, FRET in the studies of guanine-quadruplex, Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols (Methods in Molecular Biology), (Didenko, V. V. Ed.), Humana Press, Inc. (Totowa, NJ), pp. 311-341 (2006). [2] S. Sato et al., Anal. Sci., 35, 85-90 (2019). [3] K. Ohtsuka et al., Chem. Comm., 48, 4740-4742 (2012). S. Takenaka, Curr. Protoc. Nucleic Acid Chem. 62, 8.9.1-8.9.9 (2015).

R.P.13
18:00
Authors : A.A. Romansky, V.L. Karbivskyy, V.А. Dubok, S.S. Smolyak, L.I. Karbivska
Affiliations : G.V. Kurdyumov Institute for Metal Physics of the N.A.S. of Ukraine

Resume : Previously, we developed samples of biocompatible composite based on calcium phosphates – "Syntekost" (synthetic bone). The aim of this work is to optimize the properties of bioactive materials for bone tissue recovery – ensuring their oncoprotective and anti-inflammatory properties. At various temperature conditions, a number of inorganic materials were synthesized: calcium phosphate ceramics, glasses and sitalls, as well as composites based on nanodispersed calcium hydroxyapatite. The effect of synthesis temperature conditions on the structure and properties of the obtained materials is investigated. Doping of a number of samples with bioactive elements was carried out with the aim of their functionalization. Samples of conductive bioactive nanocomposites based on nanodispersed apatite and nanodispersed graphite were obtained, and their properties were studied by electrophysical methods. An analysis of the XPS data of the obtained samples showed that, depending on the conditions of glass synthesis – melt cooling rate, temperature regime, and glass doping, the binding energy of the constituent elements changes. The temperature conditions of the process for producing such materials were optimized. The relative fraction of bioactive calcium phosphates and their crystallinity correlate with synthesis conditions, in particular – temperature regime. The XPS data for the fused glass sample indicate the presence of CaSiO_3 and Mg_2SiO_4, which are associated with bio-sitalls.

R.P.14
18:00
Authors : Viraj Bhingardive1,2, Guillaume Le Saux1,2 , Avishay Edri3, Angel Porgador3, and Mark Schvartzman1,2
Affiliations : Department of Materials Engineering1, Ilse Katz Institute for Nanoscale Science and Technology2, The Shraga Segal Department of Microbiology Immunology and Genetics Faculty of Health Sciences3, Ben-Gurion University of the Negev

Resume : Nanowires have been attracting great interest for numerous applications. In particular, their dimensions that are comparable to those of biomolecules, as well as their ultra-high aspect ratio and mechanical flexibility, make them very attractive for mimicking physiological environment of cells in the fundamental studies of the cell-environment intentions. To provide nanowires with the ability of chemical signaling they are often functionalized with signaling molecules. However, such a functionalization, based on either chemisorption or physisorption of biomolecules has not been site specific. Here, we took advantage of the fact that chemical-vapor-deposition grown nanowires carry on their tips metallic catalytic nanoparticles, to achieve the first of its type site selective functionalization of biomolecules. Such functionalization comes to provide a biomimetic 3D cell environment with precisely positioned biomolecules. We have grown Si nanowires from Au catalyst. We covered Au tips with biotinylated thiols, and used Neutravidin bridge to attach to the molecules of biotinylated anti-NKp30 – an activating ligands for Natural Killer cells, which are lymphocytes of the innate immune system. To study the effect of clustering at both nanometric and micron scale on cells, we investigated the effect of continues bed of nanowires verses micropatterned areas of nanowires. Further, we varied the stiffness of nanowires by changing their length and followed its effect on cell population.

R.P.15
18:00
Authors : Qianqian Niu, Shasha Lv, Huili Shao, Suna Fan*, and Yaopeng Zhang*
Affiliations : State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Belt and Road Joint Laboratory of Advanced Fiber and Low-Dimension Materials, College of Materials Science and Engineering, Donghua University

Resume : Triboelectric nanogenerators (TENGs) convert mechanical energy into electrical energy, providing the possibility of self-powered implantable electronic devices. However, most implantable electronic devices still require an external power source. In addition, TENGs must have outstanding biocompatibility, biodegradability, and controllable degradation rate to prevent the need for a second surgery and avoid an inflammatory response. In this work, a silk nanofibril (SNF)-based bio-TENG is fabricated using a nascent SNF film (SNFF) and regenerative silk fibroin film (RSFF). To maintain the original meso/nanoscale structure of silk, SNFs with a thickness of 0.38 nm are directly exfoliated from natural silk. RSFF and SNFF have different microstructure and work functions. The output performance of the bio-TENG with the maximum voltage, current, and power density (PD) reach up to 41.6 V, 0.5 μA, and 86.7 mW/m2, respectively. The lifetime of the TENG depends on post-treatment of the RSFF package. The raw materials of the proposed TENG, including silk and magnesium (Mg), are completely biodegradable and biocompatible in vitro. With its high sensitivity and the ability to generate power from just a human pulse, the all silk-based bio-TENG may be an attractive power source for implantable self-powered electronic devices, such as pacemakers and implantable sensors.

R.P.16
18:00
Authors : Iryna Bozhyk, O.Shynkaruk (1), O.Ivanyuta , E.Buzaneva (2), D.Zabolotny, A.Karas, H. Karas (3), N. Kurgan,.V.Karbovskyy (4)
Affiliations : 1 Iplant LLC 2 TSN University of Kyiv 3 O.S. Kolomyichenko Otolargngology Institute NAMSU 4 G.V.Kurdyumov Institute for Metal Physics, SPM&RS Centre, NASU, Kyiv, Ukraine, http://iplant.com.ua/

Resume : Are given the concrete examples of the application biochips in medicine, ecology and other spheres human activity. The biochips are used for testing the cell cultures growth at the bio ceramic substrate of parameters electric impedance sensing. We has described how to run a cell cultures growth test using the prepared biochips. A control cell cultures sample and the test clean water sample are mixed with pre-measured powdered media and injected into separate channels of the biochip. Impedance readings from the sensing electrodes in each of the biochip channels are measured and compared by virtual program. Advantages are ease of use and rapid response to a broad spectrum of inorganic and organic chemicals at concentrations that are relevant to human health concerns, as well as the long-term stability of stored biochips in a ready state for testing. Applications for detector cell cultures growth at the bio ceramic scaffold for bone tissue regeneration are of rapid field-portable testing for nanomedicine

R.P.17
18:00
Authors : Geun-Tae Yun, Woo-Bin Jung, and Hee-Tae Jung*
Affiliations : Korea Advanced Institute of Science and Technology (KAIST)

Resume : Both high static contact repellency and resistance to liquid drop impact are quite necessary to achieving a high-performance omniphobic surface. The cuticles of springtails, a small insect in nature, have both of these features, which result from their hierarchical structure composed of primary nanostructures on secondary microgrooves. Despite intensive efforts, none of the previous reports that were inspired by the springtail were able to achieve both high static repellency and pressure resistance simultaneously due to a general trade-off between these features. We demonstrate for the first time a springtail-inspired superomniphobic surface showing both features by fabrication of a hierarchical structure consisting of serif-T–shaped nanostructures on microscale wrinkles. Our biomimetic engineering yielded a surface having high repellency to diverse liquids, from water to ethanol, with an apparent contact angle above 150°. The surface was also able to show extreme pressure resistance from the impacts of diverse liquid drops, which is the best pressure resistance on omniphobic surface ever reported.

R.P.18
18:00
Authors : Priya Mullick 1 , Gopal Das 2* and Aiyagari Ramesh 1*
Affiliations : 1 Department of Biosciences and Bioengineering, 2 Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Tel: 91-361-2582313; Fax: 91-361-2582349 (Gopal Das) Tel: 91-361-2582205; Fax: 91-361-2582249 (Aiyagari Ramesh) E-mail: gdas@iitg.ac.in (Gopal Das); aramesh@iitg.ac.in (Aiyagari Ramesh)

Resume : Herein, we report a biogenic route of hydroxyapatite nanoparticle (HANP) synthesis either in presence of the whole cell (WC) of the lactic acid bacteria (LAB) Lactobacillus rhamnosus GG or a malonic acid amphiphile (MA). HANPs were generated by in situ precipitation and the characteristic peaks at 3568, 1461, and 1041 cm-1 observed in FTIR analysis and the prominent peaks at around 2θ = 26° and 2θ = 33° in PXRD analysis revealed that the LAB cells and MA could generate hydroxyapatite phase. FESEM revealed that WC-HANPs were spindle-shaped and 70-110 nm in size while MA-HANPs were spherical and 22-27 nm in size. HRTEM indicated that the lattice distance for WC-HANPs and MA-HANPs was 0.28 nm and 0.29 nm, respectively, while SAED confirmed the crystallinity of HANPs. TGA analysis indicated degradation of WC-HANPs and MA-HANPs from 20°C to 800°C, with 83% and 87% residual mass, respectively. BET analysis indicated that WC-HANPs and MA-HANPs had a surface area of 170.47 m²/g and 90 m²/g, respectively, and an average pore size of 4.9 nm and 4.04 nm, respectively. Both the HANPs were non-toxic to cultured osteosarcoma MG-63 cells. Fluorescence microscopic analysis revealed that the MG-63 cells grown in presence of HANPs appeared elongated, suggesting that HANPs likely support bone cell differentiation. The biogenic route of HANP synthesis outlined herein is an interesting prospect to generate potentially biocompatible nanomaterials for tissue engineering applications.

R.P.19
18:00
Authors : Alina Sharova, Mario Caironi
Affiliations : Dipartimento di Fisica, Politecnico di Milano, P.zza L. da Vinci 32, 20133 Milan, Italy; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia, Via Pascoli 70/3, 20133 Milan, Italy

Resume : The use of natural and bioinspired materials is an emerging key approach towards development of new-generation safe technology. Going beyond the traditional concept of electronic devices, we convey the idea of making electronics edible. This unconventional approach exploits the electronic properties of natural and food-based materials for developing ingestible functional devices. Critical biomedical, pharmaceutical, and food industry applications are targeted by the proposed field. In this framework, we explore the potential of cost-effective and edible substance, honey, to be used as electrolytic gate viscous dielectric. Honey-gated organic field effect transistors (OFETs) based on both n & p type semiconductors are fabricated. A distinctive feature of these transistors is their long-term stability, reproducibility and low voltage < 1V operation in air. Devices exhibit forward-looking electronic performances, notably, electron and hole mobility–capacitance product of 3.5 × 10–3 μF/Vs and 23 × 10–3 μF/Vs, respectively, surpassing ones of the previously reported water-gated OFETs. Furthermore, the observed devices responsivity to humidity provides promising opportunities for sensing applications. We then demonstrate, for the first time, the implementation of honey-based integrated circuits: inverting logic gate and ring oscillator. Lastly, honey-gated OFETs are fabricated on edible flexible tattoo-paper substrate that acts as a versatile platform for organic edible electronics [1]. [1] Giorgio E. Bonacchini, Caterina Bossio, Francesco Greco, Virgilio Mattoli, Yun‐Hi Kim, Guglielmo Lanzani, and Mario Caironi, Tattoo‐Paper Transfer as a Versatile Platform for All‐Printed Organic Edible Electronics, Advanced Materials 30 (14): 1706091, 2018

R.P.20
18:00
Authors : Hsing-An Lin1,2,3, Yoshikatsu Sato4, Yasutomo Segawa2,3, Taishi Nishihara2,3, Kakishi Uno3, Lawrence T. Scott5, Tetsuya Higashiyama3,4, Kenichiro Itami2,3,4
Affiliations : 1 School of Materials Science and Engineering, Shanghai University, Shanghai, 200444, China. 2 JST ERATO, Itami Molecular Nanocarbon Project, Chikusa, Nagoya 464-8602, Japan. 3 Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan. 4 Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Japan. 5 Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467-3860, USA

Resume : Incorporating hydrophilic tetraethylene glycol with saddle-shaped nanographene make water-soluble warped nanographene (WNG) has good solubility in organic solvents and water. It exhibits strong green-yellow fluorescence, high photostability, and low cytotoxicity. The aforementioned properties imply that water-soluble WNG is applicable to live-cell imaging. Subsequently, water-soluble WNG was applied for HeLa cell imaging. The results indicated that water-soluble WNG internalized in HeLa cells and accumulated into the lysosome as aggregates. During live-cell imaging by water-soluble WNG, we serendipitously discovered that the HeLa cells stained with water-soluble WNG could be killed by selective laser irradiation. In the presence of 5.0 mM of water-soluble WNG, the light irradiated HeLa cell shrunk, generated blebs, and finally died. Moreover, dose-depended cell viability is observed. The cell viability was 98% in the presence of 0.01 mM of water-soluble WNG. Upon increasing the concentration of water-soluble WNG from 0.01 to 1.0 mM, a dramatic decrease in cell viability was observed, and 91% of the cells were killed in the presence of 1.0 mM water-soluble WNG under irradiation. Using 5.0 mM water-soluble WNG, almost no cells survived under the irradiation. In contrast, even in the presence of water-soluble WNG, no obvious change in cell morphology and viability was observed without light stimulation. Although the mechanism is unclear, the relatively high efficiency of the singlet oxygen generation of water-soluble WNG may contribute to its HeLa cell death.

R.P.21
18:00
Authors : Valerio Voliani
Affiliations : Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, P.zza San Silvestro, 12 - 56127, Pisa (PI), Italy.

Resume : Noble metal nanomaterials hold the promise to shift the current medical paradigms for the treatment and diagnosis of several diseases, among which neoplasms and infections, due to their peculiar chemical, physical and physiological behaviors. Despite the massive efforts, treatments based on inorganic nanomaterials are mainly at the preclinical stage, due to the body persistence issue. Indeed, non-biodegradable materials persist within excretion system organs for long-term: that is not acceptable for exogenous drugs. The most recent progresses in the design, production and application of noble metal nanomaterials able to escape from the organism after the designed action will be discussed together with their biokinetics, and biosafety. The research leading to these results has received funding from AIRC under MFAG 2017 – ID 19852 project – P.I. Voliani Valerio.

R.P.22
18:00
Authors : Sangita Kundu, and Nilmoni Sarkar
Affiliations : Senior Research Fellow, Department of Chemistry, Indian Institute of Technology Kharagpur-721302 West Bengal India; Professor, Department of Chemistry, Indian Institute of Technology Kharagpur-721302 West Bengal India

Resume : In vivo thermometry is an emerging area of research to understand the temperature regulation within and between the organs and to describe the intricate cellular metabolic processes. Highly green luminescent gold nanoclusters (Au NCs) have been successfully implemented as potential sensors for temperature measurement in vivo because of their small size, biocompatibility, quick equilibration with temperature and most importantly their response reflect the thermal environment in the physiological temperature range. This thermoresponsive fluorescence lifetime based nanoprobe based on the supramolecular host-guest recognizition employing 6-aza-2-thiothymine and L-arginine (L-Arg) shows selective cytoplasm staining and robust response to temperature. Subsequently, fluorescence lifetime imaging microscopy (FLIM) has been employed to elucidate intracellular nanothermometry inside living cells. In depth understanding of protein-Au NCs interaction offer a promising avenue to rationally design target-specific fluorescent biolabeling agent. Because of the small surface area of the Au NCs, these probes open up the new possibility of combining therapeutic moieties having the specific cytoplasm targeting capability and simultaneously monitor the temperature assisted drug- delivery/release.

R.P.23
18:00
Authors : Hyoung-Joon Jin
Affiliations : Inha University

Resume : Recently, nanocellulose has gained much attention as a potential reinforcing material in the field of composites owing to its excellent mechanical, biodegradable, renewable, and biocompatible properties. Cellulose nanocrystals (CNCs), which combine natural abundance and excellent mechanical properties, are a promising candidate for use as polymer reinforcement agents. CNCs are employed in a variety of fields to enhance the mechanical properties of products such as cosmetics, pharmaceuticals, paper coatings, and additives in paper and paperboard. The high density of hydroxyl groups provides the hydrophilic properties of these materials, making them also suitable for the production of hydrogels. However, because of their hydrophilic nature, CNCs are mainly used to control the hydrophilic properties and improve the mechanical properties of hydrophobic polymers, especially synthetic polymers. On the other hand, in the case of hydrophilic polymers, an additional physicochemical cross-linking process is required because CNCs only affect the mechanical properties of composite materials. In this work, dual functionalized-CNCs as a nanofiller was used to develope a gelatin-based nanocomposite film with enhanced mechanical properties and hydrolytic stability . Through the sodium periodate oxidation reaction, C2-C3 cleavage of cellulose molecules was generated and the aldehyde groups were introduced on these carbon atoms. Using dialdehyde CNCs, interfacial covalent bonding was formed between the aldehyde group of the CNCs and the amine group of the gelatin molecule through a nucleophilic addition reaction. To investigate the effect of the dialdehyde CNC content on the properties of the biocomposite, the mechanical and physicochemical properties and the hydrolytic stability of the fabricated biocomposite films were studied using various analysis techniques. The bi-functional CNCs, which can have both cross-linking and reinforcing effects, would expand the fabrication and exploration of high-performance biopolymer nanocomposites. The gelatin-based nanocomposite film developed by this strategy has greatly improved mechanical properties and hydrolytic stability, which suggests that gelatin can be used not only as a hydrophilic polymer but also as a universal polymer.

R.P.24
18:00
Authors : Doohun Kim, Kyeong-Hee Lee, Bongju Kim, Minwoo Kim
Affiliations : Korea Electro-technology Research Institute (KERI), Changwon 51543, Republic of Korea; Dental Life Science Research Institute & Clinical Translational Research Center for Dental Science, Seoul National University Dental Hospital, Seoul 03080, Republic of Korea; Dentis Ltd., Daegu 41065, Republic of Korea

Resume : To increase bone regeneration around a titanium implant, nanopattern surfaces with 10 nm nanopores in 120 nm dimples were produced by electrochemical nanopattern formation (ENF). The ENF surfaces were obtained by removing the TiO2 nanotube layers prepared by an anodization process. To determine the effect of the ENF surface in vitro, cell proliferation assays, alkaline phosphatase (ALP) activity assays, Alizarin red staining, western blotting, and immunocytochemistry were performed. Atomic force microscopy and scanning electron microscopy analysis showed that the ENF surface had an ultrafine surface roughness with highly aligned nanoporous morphology. In vitro, human mesenchymal stem cells exhibited increased cell proliferation and enhanced expression of osteogenic molecules such as ALP, osteopontin, and osteocalcin on ENF surfaces compared to the TiO2 nanotube surfaces. Therefore, ENF is a promising technique for coating osteointegrative implant materials.

R.P.25
18:00
Authors : Siyu Xiong, Prof Andrew Davenport, Prof Kwang-Leong Choy
Affiliations : Siyu Xiong, Prof Kwang-Leong Choy: Institute for Material Discovery, University College London, London, WC1E 7JE, United Kingdom Prof Andrew Davenport: Centre for Nephrology, Royal Free Hospital, London NW3 2PF, United Kingdom

Resume : Indoxyl sulfate (IS) has been reported to not only accelerate the progression of chronic kidney disease (CKD), but also increase the risk of cardiovascular disease by increasing the oxidative stress of CKD patients. Current haemodialysis facilities are immobilized due to huge dialysis machine and dialysate bag. Some wearable artificial kidney (WAK) devices are still based on diffusion principles, which could not fundamentally solve the problem of portability. We report on a novel IS absorbent material based on biocompatible polycaprolactone-chitosan (PCL-CS) nanocomposite fibrous structure. This composite absorbent material has a high internal surface to volume ratio so as to greatly increase the interaction between the IS and chitosan, which exhibits character of high binding affinity to IS molecules. The PCL-CS absorbent material was fabricated by 2D-controlled close-range electrospinning. The surface morphology and chemical composition were characterized by a combination of scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The IS molecule absorption of the PCL-CS absorbent was quantitatively evaluated. This novel nanocomposite absorbent may provide new sights for the removal of metabolic toxins in a portable way.

R.P.26
18:00
Authors : Kaiwei Tang(1)*, Guomin Wang(1), Paul K. Chu(1).
Affiliations : (1) City University of Hong Kong, China

Resume : Au decorated aligned ZnO nanorods are synthesized on the Ti substrate by the silane coupling agent-assisted seed layer formation and hydrothermal process with the following magnetron sputtering of gold nanoparticles. The Au@ZnO systems inactivate about 80% of Escherichia coli in the first 1h culture in the dark through an extracellular electron transfer mechanism instead of ROS production and zinc ion releasing. Electrons on the respiratory chains of the bacterial membrane transfer to the surface of the materials, making bacteria lose electron until death. At the same time, the transferred extracellular electrons convert into the bacterial current by the electrochemical workstation, primarily demonstrating the property of the materials to function as a sensor of bacteria counting and status observation. This work sheds light on the detailed understanding of antibacterial behaviors of ZnO-based materials especially at the early stage of bacteria-material contact and provides insights for the designing of devices with both antibacterial and detection applications.

R.P.27
18:00
Authors : Tae Ann Kim*, Jun Beom Pyo, Sang-Soo-Lee, and Min Park
Affiliations : Photo-electronic Hybrids Research Center, Korea Institute of Science and Technology, Republic of Korea

Resume : Developing materials that can absorb large amounts of strain without fracture or significant degradation in their electronic properties is important to realize stretchable electronics. Here, stretchable conductors made from reduced graphene oxide/polydimethylsiloxane (r-GO/PDMS) composite are fabricated to achieve stable resistance changes under uniaxial stretching. By controlling the weight fraction of GO in water, highly aligned and porous GO structure is obtained over 0.5 wt% of GO solution. After thermal and chemical reduction of GO to r-GO film, PDMS is infiltrated into pores of the film to make free-standing and elastic composites. Electrical percolation of r-GO in the composite is achieved at the r-GO concentration over 0.3 wt% along with improvement of electrical conductivity. However, highly aligned r-GO/PDMS composite only exhibits notable suppression of resistance change under the large deformation of 100% strain. The resistance change of 0.3 wt% r-GO/PDMS composites at 100% strain is over 7 times higher than that of 0.7 wt% r-GO/PDMS composites, exhibiting the dramatic effect of r-GO alignments. Even after repeated stretching/releasing cycles, the variation in resistance from 0 to 70% of strain is stable. The stretchable conductor based on the aligned r-GO/PDMS composite was successfully applied as a resistive strain sensor.

R.P.28
18:00
Authors : N. Tsierkezos, U. Ritter, P. Scharff (2), O.Ivanyuta, E. Buzaneva (1)
Affiliations : 1National Taras Shevshenko University of Kyiv, 64/13, Vladimirska Str., 01033, Kiev, Ukraine e-mail: iva@univ.net.ua 2 Technische Universitat Ilmenau, Institut fur Chemistry & Biotechnoly, Postfach 100565, 986884 Ilmenau, Germany

Resume : The trend of organic thin films research toward conductivity - photovoltaic chip has allowed using the/films from ds-DNA –templated naocarbons molecular layers obtained by biotechnology. On the base of the review deals to analysis of electronic properties, photosensitivity, photoelectron moving force (PhEMF) and their stability under UV-vis irradiation for nanocarbon films with DNA molecules, we selected the thin films from silf-assembled layers of fullerene C60/C60 oxygen derivatives/ds-DNA on silicon for the detail investigations. The developing model of conductivity, and photovoltaic effect, in these layerss takes in account that C60 molecule is an acceptor of electrons. And the effect enhances with formation of C60 oxygen derivatives: 6-5 open fullerene C60 as we showed in first time [1].The evidence of self-assembling of these layers with (ds-DNA) in the nanostructured films on Si surface were obtained on the base of STM and SEM images of the films with the assembles (8-10 and 30-40 nm in diameter).The conductivity of the films was modulated by diode characteristics of fullerene C60/6-5 open fullerene C60 and C60/ ds-DNA contacts for n-type semiconductors fullerenes witch were determined by STP - tunneling spectroscopy. The discovered dynamic behavior of photosensitivity to 200-400 nm irradiation and PhMF appearance (0,25-0,37 eV) at 400-1000 nm irradiation (during 10 min - 1 h) of these films with several structures allow to consider these nanostructured layers/films as conducting/ photovoltaic chips. The examples for an application of these chips based on conductivity/photovoltaic models which have been developed for organic nanostructured thin films from C60 fullerene/ds-DNA molecular assemblies are discussed. Ref.,{1] E. Buzaneva, A. Gorchinskiy, P. Scharff, K. Risch, A. Nassiopoulou, C. Tsamis, Yu. Prilutsyy, O. Ivanuta, A. Zhugayevych, D. Kolomiyets, A. Veligura, DNA, DNA/Metal Nanoparticles, DNA/Nanocarbon and Macrocyclic Metal Complex/Fullerene Molecular Building Blocks for Nanosystems: Electronics and Sensing, In Book Frontiers of multifunctional integrated nanosystems, Eds: Eugenia Buzaneva and Peter Scharff, NATO Science Series, II-Mathematics, Physics and Chemistry–Vol 64, Kluwer Akademic Publishers, Dordrecht, 251-276, 2004.

R.P.29
18:00
Authors : Wiktoria Lipińska (1)*, Katarzyna Siuzdak (1), Piotr Barski (2), Agnieszka Lindstaedt (2), Katarzyna Grochowska (1)
Affiliations : (1) Centre for Plasma and Laser Engineering, The Szewalski Institute of Fluid-Flow Machinery, Fiszera 14, 80-231 Gdańsk, Poland; (2) Prochimia Surfaces, Zacisze 2, 81-823 Sopot, Poland

Resume : Nowadays, diabetes became a serious problem since everyday 400 million people face with it. Following that, extensive research works towards the enzymatic electrochemical biosensors are undertaken since those devices can selectively monitor glucose level in human body fluids. In this work, preparation method and electrochemical activity of the enzyme-functionalized Au-Ti electrode are presented. Firstly, the conductive platform was fabricated via anodization process of Ti and further chemical etching that results in regularly distributed nanodimples. Secondly, the thin Au film sputtering followed by the thermal annealing leads to the Au nanoparticles formation in the host Ti hollows revealed by scanning electron microscopy. Thirdly, the surface of obtained material was modified with glucose oxidase by the hybrid immobilization route. The first layer was the self-assembled organic monolayer composed of HSC11H22EG6OCH2COONHS chains. Then, GOx enzyme was anchored via the N-hydroxysuccinimide ligand using the cross-linking immobilization route. Basing on the cyclic voltammetry measurements performed in PBS the sensitivity of 43.926 μMcm-2mM-1 was reached within glucose concentration range of 0.006-2.345 mM while the limit of detection was as low as 5.331 μM. Additionally, the measurements in artificial sweat and saliva as well as human serum were carried out to show the performance in the real conditions. Research is financed by NCBR via LIDER/2/0003/L-8/16/NCBR/2017 grant.

R.P.30
18:00
Authors : Aishee Dey* (1), Preetam Guha Ray (2), Santanu Dhara (2) & Sudarsan Neogi (1)
Affiliations : (1) Department of Chemical Engineering, Indian Institute of Technology Kharagpur, India - 721302 (2) School of Medical Science and Technology, Indian Institute of Technology Kharagpur, India - 721302.

Resume : Nanosized zinc oxide is gaining importance in the biomedical field due to its commendable antibacterial efficacy. The increased specific surface area of the nanoparticles ensures enhanced particle activity, hence proving itself to be more effective than the bulk sized particles. The fluorescence property of zinc oxide arised due to defects and its use in biomedical field is recently being explored. In this work, shape controlled fluorescent zinc oxide nanoparticles was synthesized by a facile and novel wet chemical technique. Morphological and physico-chemical characterization of the nanoparticles were performed in order to affirm structural and functional aspects of the nanoparticles. Further, bactericidal activity of the 30 nm sized ZnO nanoparticles were tested against B. subtilis and E. coli. It was observed that 100 ppm conc of the nanoparticles were able to demonstrate 99% (5 log reduction) in bacterial colony size in 6 h of incubation time. Similar results were also witnessed in zone of inhibition, growth curve analysis, DNA leakage assay and live-dead assay, thus confirming bactericidal efficacy of the nanoparticles. Further, in vitro cytocompatibility was also performed with L929 fibroblast cells in order to evaluate toxicity profile of the nanomaterial. The excellent bactericidal efficacy coupled with low toxicity profile renders the fluorescently active sub-50 nm sized ZnO nanoparticles as an excellent alternative for next generation non-invasive biomedical applications. References 1. Ray, Preetam Guha, et al. "Sonication Assisted Hierarchical Decoration of Ag-NP on Zinc Oxide Nanoflower Impregnated Eggshell Membrane: Evaluation of Antibacterial Activity and in Vitro Cytocompatibility." ACS Sustainable Chemistry & Engineering 7.16 (2019): 13717-13733. 2. Alekish, Myassar, et al. "In vitro antibacterial effects of zinc oxide nanoparticles on multiple drug-resistant strains of Staphylococcus aureus and Escherichia coli: An alternative approach for antibacterial therapy of mastitis in sheep." Veterinary world 11.10 (2018): 1428.

R.P.31
18:00
Authors : Gonen Ashkenasy Nathaniel Wagner Indrajit Maity
Affiliations : Ben-Gurion University of the Negev, Beer Sheva, Israel

Resume : Like many other open systems in nature, living organisms are replete with rhythmic and oscillatory behaviour at all levels, to the extent that oscillations have been termed as a defining attribute of life. Recently, we have started to investigate the “bottom-up” construction of peptide-based networks that display bistable behaviour and oscillations. We have utilized replicating coiled coil peptides, which have already served to study emergent phenomena in complex mixtures. In this talk, we will first describe the kinetic behaviour of coupled oscillators, producing various functions such as logic gates, integrators, counters, triggers and detectors.[1] These networks were also utilized to simulate the Kai-proteins circadian clocks, producing rhythms whose constant frequency is independent of the input intake rate and robust towards concentration fluctuations.[2] Then, we shall disclose our recent experimental results, showing for that the peptide replication process also lead to bistability in product equilibrium distribution.[3-5] We propose that these recent studies may help further reveal the underlying principles of biological clocks, and pave the way to exploit synthetic oscillators for switching and signalling functions. 1. Wagner et al. J. Phys. Chem. Lett. 2015, 6, 60. 2. Gurevich et al. Chem. Commun. 2015, 51, 5672. 3. Mukherjee et al. Angew. Chem. Int. Ed. 2015, 54, 12452. 4. Wagner et al. ChemPhysChem 2017, 18, 1842. 5. Maity et al. Nature Commun. 2019, 11, 681.

R.P.32
18:00
Authors : Wenyi Hu* (1), Yuval Elani (2), and Claudia Contini (1).
Affiliations : (1) Department of Chemistry, Imperial College London, London, UK (2) Department of Chemical Engineering, Imperial College London, UK.

Resume : The current state of the art in artificial cell science relies on the construction of lipid vesicle membranes whose inherent properties, however, limit their applications particularly in sustaining cell internal biochemistry and communication between neighbors. Polymersomes are considered as ideal alternatives to liposomes because of their enhanced stability, chemical versatility, chemical and mechanical resistance. Nevertheless, the use of polymeric vesicles is restricted due to their low membrane permeability. Here, a suite of porous polymer membranes which is intrinsically permeable to molecules of defined sizes is explored as chassis for artificial cell, with the ideal functionalities of retaining specific molecules (e.g. protein and DNA) but allowing influx and efflux of small molecules (e.g. metabolites, signal molecules and mRNA). The porous vesicles are expected to form directly by self-assembly of copolymers, and the porosity is hypothesized to be controlled by varying experimental parameters during extrusion. The combinations of copolymers involve the mixtures of diblock copolymer PEG-PBD and triblock copolymer PEG-PPO-PEG with different molar ratios, and the solutions of two or more triblock copolymers PEO-PPO-PEO with different head-to-tail ratios. The porous polymer vesicle is novel in artificial cell science; thus, many potential applications can be tapped into, including for spatially-segregated biochemical reactions in polymer-based synthetic organelles.

R.P.33
18:00
Authors : Xiaozhen Li, Shuang Tian, Zhongming Huang, Yafang Xiao, Xiao Cui, Chun-Sing Lee
Affiliations : City University of Hong Kong

Resume : High-performance photothermal agents remain highly required for realizing efficient tumor photoelimination. However, low-photoconversion and non-biodegradability of photothermal materials seriously lead to restricted tumor suppression or unavoidable biosafety issues. In such, a major mission is focused on developing efficient and dependable agents with prominent photothermal conversion efficiency for maximized cancer photoelimination. Herein, we developed a biodegradable photothermal therapeutic (PTT) agent, π-conjugated oligomer nanoparticles (F8-PEG NPs), for highly efficient cancer theranostics. By exploiting an oligomer with excellent near-infrared (NIR) absorption, the nanoparticles show a high photothermal conversion efficiency (PCE) up to 82% surpassing those of reported inorganic and organic PTT agents. In addition, the oligomer nanoparticles show excellent photostability on one hand but also good biodegradability. The F8-PEG NPs are also demonstrated to have excellent biosafety and PTT efficacy both in vitro and in vivo. This contribution not only proposes a promising oligomer-based PTT agent, but also provides insight into developing highly efficient nanomaterials for cancer theranostics.

R.P.34
18:00
Authors : Xiao Cui,Yafang Xiao,Xiaozhen Li,Shuang Tian,Qi Zhao
Affiliations : Department of Chemistry, Institution Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, Address 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, P. R. China

Resume : Exploitation of new photosensitizes with sufficient oxygen-independent O2−• generation is highly desirable for overcoming hypoxia in photodynamic therapy (PDT). Herein, we report the first demonstration using a radical molecule as a new photosensitizer for hypoxic-overcoming photodynamic therapy. After self-assembling the radical molecules into nanoparticles (NPs), the NPs show good water dispersibility, good biocompatibility, board near infrared (NIR) absorption and emission at ~ 800 nm. Significantly, radical NPs remain stable in various biological solution, 100-days exposure to ambient environment, and long-term laser irradiation, which are ultrastable compared to many reported radical materials. Efficient 1O2 and O2−• generation and cytotoxicity were observed upon NIR irradiation. More importantly, even under hypoxia condition, sufficient oxygen-independent O2−• generation and cytotoxicity were observed addressing the most important hurdle for successful PDT in oxygen-deficient tumor microenvironment. This work demonstrates that stable radical molecules could have interesting properties suitable for novel biomedical applications.

R.P.35
18:00
Authors : Yafang XIAO, Xiao CUI, Shuang TIAN, Xiaozhen LI, Qi ZHAO.
Affiliations : Department of Chemistry Institution Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, Address 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, P. R. China

Resume : Rational manipulation of energy utilization from excited-state radiation of theranostic agents with donor-acceptor structure is relatively unexplored. Herein, we present an effective strategy to tune the exciton dynamics of radiative excited state decay for augmenting two-photon nanotheranostics. As a proof of concept, two thermally activated delayed fluorescence (TADF) molecules with different electron-donating segments are engineered, which possess donor-acceptor structures and strong emissions in the deep-red region with aggregation-induced emission characteristics. Molecular simulations demonstrate that change of the electron-donating sections could effectively regulate the singlet-triplet energy gap and oscillator strength, which promises efficient energy flow. Two-photon laser with great permeability is used to excite TADF NPs to perform as theranostic agents with singlet oxygen generation and fluorescent imaging. These unique performances enable the proposed TADF emitters to exhibit tailored balances between two-photon singlet oxygen generation and fluorescence emission. This result demonstrates that TADF emitters can be rationally designed as superior candidates for nanotheranostics agents by the custom controlling exciton dynamics.

R.P.36
18:00
Authors : Shi Mo (1), Paul K. Chu, *(1), Kaiwei Tang (1), Babak Mehrjou (1), Guomin Wang (1), Huaiyu Wang (2).
Affiliations : (1) Department of Physics, City University of Hong Kong, Hong Kong, China; (2) The Shenzhen Institutes of Advanced Technology, The Chinese Academy of Science (CAS), China.

Resume : Sheet-like structures such as nanoflake and nanolamellae are of great interest in biomedical engineering, because the sharp edge or large surface area can affect the bacterial and cellular behaviors on attaching to these structures. The nanolamellar arrays can be readily constructed on the surface of polyetheretherketone (PEEK) through one-step plasma treatment, showing a re-crystallization and time-dependent growth process. The gap, thickness and size of the nanolamellar array are controlled by adjusting the volume of plasma injection and the treatment time, which in turn affect the antibacterial activity and the growth of osteoblasts. The sharp edge and dimensional parameters of the nanolamellar arrays contribute to the antibacterial activity. However, due to the large size of the osteoblast and the stiffness of the cell membrane, the osteoblasts can grow on the arrays, which is affected by the dimension of the arrays. The improved osteoblast proliferation also renders it desirable osteointegration activity. The overall results in this work offer insights into the antibacterial behavior and the osteogenesis of the nanolamellar arrays, thus assuring this type of structure better application in biomedical engineering.

R.P.37
18:00
Authors : Ting-Yu Lu, Ching-Cheng Tsai, and Jiashing Yu*
Affiliations : Department of Chemical Engineering, National Taiwan University

Resume : Human adipose-derived stem cells (hASCs) can differentiate into multiple lineages and be harvested abundantly. However, the expression of pluripotency markers, which is important for the renewal and differentiation capabilities of hASCs, decreases during monolayer culture. Increasing evidence has proven that cells aggregated to form cell spheroids in 3D cell cultures better mimic the in vivo microenvironment and can enhance the expression of stemness markers. In this study, first, uniform hASC spheroids were formed by seeding cells in agarose microwell plates, and the size of the spheroids could be adjusted. Most importantly, the stemness expression of the spheroids increased significantly. Additionally, we utilized microbial transglutaminase (mTG), which is an enzyme that exhibits highly specific activity over a wide range of temperature and pH, to crosslink gelatin. The enzymatic crosslinking reaction is milder than physical and chemical methods, which may lead to cell death. The properties of the gelatin/mTG hydrogel were evaluated in detail. In addition, the spheroids were encapsulated in the 3D hydrogel successfully. The results showed that the hydrogel has low toxicity to the cells, which significantly proliferated in the 3D hydrogel. Moreover, the analysis of the differentiation potential indicated that the cell spheroids in the 3D hydrogel exhibited good activity, especially adipogenesis and chondrogenesis, compared to the cell suspension group. Furthermore, the in vivo data confirmed the excellent injectability and biocompatibility of the 3D hydrogel. Second, we demonstrated that cell spheroids entrapped in gelatin hydrogel can accelerate wound healing. To evaluate the in vivo effects of these spheroids/hydrogel system, we applied hASC single cells/hydrogel and hASC spheroids/hydrogel in a designed rat skin repair model. Results showed that skin wounds treated with hASC spheroids hydrogel system had faster-wound closure and a significantly higher ratio of angiogenesis. Based on the in vitro and in vivo results, the self‐assembled hASC spheroids hydrogel system may be a promising cellular source for skin tissue engineering and wound regeneration.

R.P.38
18:00
Authors : Seungje Lee*(1), Minji Ko(1), Soomin Ahn(1), Seo Yeon Shin(1), Gang Yeol Yoo(2), Woong Kim(2) and Young Rag Do(1)
Affiliations : (1) Department of Chemistry, KOOKMIN University, Seoul, Republic of Korea, (2) Department of Materials Science and Engineering, Korea University

Resume : Multifunctional anti-reflective (AR) SiO2 nano-rod structures were fabricated on glass on an ITO substrate using nano-spheres in a hexagonal-closed-packing array in an attempt to improve the efficiency of light extraction. Compared to the reflection of flat ITO/glass substrates, surface reflection of AR patterned ITO/glass decreases with gradual change of refractive index, resulting in an increase in the amount of transmitted light. SiO2 nano rod structures were fabricated by a combination of polystyrene (PS)-based nano-sphere lithography (NSL) and reactive ion etching (RIE). Dimensions of height and diameter can be adjusted by selective gas etching process. O2, CHF3, and Ar gas were selected to fabricate the SiO2 nano structure. Finally, moth-eye patterned AR SiO2 nano structures were fabricated. The measurement results for our nanostructure (diameter: 150 nm, height: 400 nm) showed a 2% reflectance reduction in the 380~780 nm range compared to reflectance of flat ITO/glass substrate. Due to the deposition of SiO2 layer by Plasma Enhanced Chemical Vapor Deposition (PECVD), super hydrophilic nano patterns were obtained. To obtain an anti-fogging effect, we performed self-assembled monolayers (SAMs) treatment by applying perfluoro siloxane (PFS) on AR patterned SiO2 nanostructure. A field emission scanning electron microscope (FE-SEM) was used to investigate the morphology of the AR-patterned SiO2 nanostructure. Hydrophobicity was measured by contact angle measurement.

R.P.39
18:00
Authors : Miryam Criado-Gonzalez1,2; Alejandro Hernandez-Sosa3; Maria Rosa Aguilar3,4; Luis Rojo3,4; Fouzia Boulmedais1; Rebeca Hernández3
Affiliations : 1. Université de Strasbourg, CNRS, Institut Charles Sadron, Strasbourg, France 2. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, Strasbourg, France 3. Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Madrid, Spain 4. Biomedical Research Networking Center in Biomaterials, Bioengineering and Nanomedicine, CIBER-BBN, Monforte de Lemos, 5. 28029 Madrid, Spain

Resume : We report on the development of interpenetrating polymer networks (IPN) for specific application in osteochondral regeneration based on the employment of Localized Enzyme Assisted Self-Assembly (LEASA) systems on hydroxyapatite nanoparticles (HA-NPs) as building blocks for the construction of 3D peptide based hydrogels and their combination with alginate hydrogels crosslinked with bioactive cations: Ca2+, Zn2+ and Sr2+. Silica nanoparticles have been recently reported to control the self-assembly of a peptide based hydrogel onto their surface by enzymatic transformation of non-interacting peptides into hydrogelators. To the best of our knowledge, this is the first time that HA nanoparticles are employed as templates for peptide gelation. The combination of alginate and a peptide network as an IPN gives rise to a bioactive material able to be printed, with modulated mechanical properties depending on the concentration of HA nanoparticles and the crosslinker: polymer ratios. As a first step, UV-vis experiments demonstrated the successful adsorption of alkaline phosphatase (ALP) onto HA-NPs. A gel was obtained after 24 hours when ALP-HA-NPs were put in contact with a solution of Fmoc-FFpY peptide. Then, the formation of the IPN was ascertained through morphological and rheological measurements. The determination of the yield stress, thixotropic properties and gelation time allowed to assess the suitability of the proposed material for bioprinting.

R.P.40
18:00
Authors : Dr., Docent Oleksandr .Ivanyuta
Affiliations : Taras Shevchenko National University of Kyiv,64/13, Volodymyrska Str., Kyiv, 01601, Ukraine iva@univ.net..ua

Resume : Excessive microwave exposure in the early 1970s led to injuries and fatal accidents. The higher the power of microwave radiation, the greater the pathological changes in nerve cells. The study aims to investigate the effect of microwave radiation on botanic and animal DNA. [1] DNA of lily flowers and cattle were selected for this study. DNA were exposed for 1 min a day 10 h for 15 days at 1 - 30 GHz frequency (power density, 0.1 µW / cm (2)). The whole-body specific absorption rate was estimated to be 5 µW / kg. Exposure took place in a ventilated resonator surface wave placed in a rectangular waveguide. After completion of the exposure period, DNA were samples conductivity was measured. Experiments were performed in a blind manner and repeated. After completion of the exposure period, DNA were samples conductivity was measured. Experiments were performed in a blind manner and repeated. A significant decrease conductivity was after 9 days of exposure for DNA lily flowers as compared with DNA cattle after 14 days exposed. The study concludes that a reduction in conductivity or an increase in sample volume may cause significant damage in brain due to chronic exposure of these radiations. I report on a new method of electrodynamic modeling of the complex biological objects. The method is based on the computer experiment realized with CAD systems. One-way analysis of variance method was adopted for statistical analysis. Simulations have with CAD systems shown that microwaves in the frequency range from 1 to 30 GHz can penetrate the cranium and that <20% of them can penetrate the deep brain, where they can penetrate <1-4 cm into the brain. These biomarkers clearly indicate possible health implications of such exposures. The great attention is given to the influence of microwave electromagnetic field on a human body, first of all, on a human head. 1. Kesari KK, Kumar S, Behari J. Pathophysiology of microwave radiation: Effect on rat brain. Appl Biochem Biotechnol. 2012;166:379–388

R.P.41
18:00
Authors : Ziqi YU, Kirk Schanze
Affiliations : Department of Chemistry, University of Texas, San Antonio

Resume : Fungal infections caused by Candida albicans have significantly increased through the formation of biofilm on living tissues and indwelling medical devices. These infections have huge clinical implications since fungal biofilm displays increased resistance to antifungal agents, therefore, it shows significant risk to immunocompromised patients.1 Morbidity and health care costs related to fungal infections has become a serious medical problem. Traditional treatments for Candida infections are associated with drugs that show high toxicity and antimicrobial resistance. Developing new antimicrobial agents to disrupt fungal biofilm has become an urgent need in the current scenario. The booming development of water-soluble conjugated polymers in the past decades2,3 has shown promising effects against bacteria. In this context, water-soluble conjugated polymers functionalized with benzimidazolium groups were synthesized by Sonogashira coupling, and antimicrobial properties against Candida albicans were studied. The conjugated polymers differ by the nature of the backbone such as polyfluorene(PF-PPE), poly(para-phenylene ethynylene)(p-Benzimi-PPE), poly(meta-phenylene ethynylene)(m-Benzimi-PPE). The photophysical properties of the polymers have systematically investigated.4 PF-PPE display absorption maximum at 380 nm and emission maximum at 475 nm, fluorescence quantum yield is φH2O=0.05, φMeOH=0.098. The antimicrobial activities of the three main chain types of conjugated polymers irradiated at visible light 420 nm which decreased in the order: PF-PPE > p-Benzimi-PPE > m-Benzimi-PPE. Under visible light irradiation, the PF-PPE showed pronounced antifungal properties against Candida albicans under all three modalities (planktonic, biofilm inhibition and biofilm eradication.

R.P.42
18:00
Authors : Susloparova, A.* (1), Mahdi, G. (1), Guérin, D. (1), Halliez, S. (2), Colin, M. (2), Buée, L. (2), Coffinier, Y. (1), Dargent, T. (1), Alibart, F.(1,3), Pecqueur S. (1).
Affiliations : (1) Univ. Lille, CNRS, Centrale Lille, Yncréa ISEN, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000 Lille, France (2) Université de Lille, Univ. Lille, Inserm, CHU-Lille, Jean-Pierre Aubert Research Centre (JPARC, UMR - S 1172), 59045 Lille, France (3) Laboratoire Nanotechnologies & Nanosystèmes (LN2), CNRS, Université de Sherbrooke, J1X0A5, Sherbrooke, Canada.

Resume : One great challenge for bio-sensing is to ensure that detection/transduction of biochemically rich systems is fully mastered to reliably record relevant information descriptors. For neuro-sensing with microelectrode arrays (MEAs), signal transduction lies at the interface between extracellular ionic currents and the electrodes. By chemically/morphologically engineering electrode materials’ interface to ensure the best electrochemical surface impedance, the aim is to find the right materials that detect ionic signals from neurons and transduce them into electronic signals with the lowest information loss. In this direction, we show how to lower the surface impedance of microelectrodes by electropolymerization of synthesized thiophene-based monomers, functionalized for higher cell biocompatibility and higher electrochemical performances. By in-situ monitoring of impedance change with voltage-ramped impedance spectroscopy, we systematically screened electrical and chemical deposition conditions to control the materials’ morphology (confirmed by atomic force and electron microscopy) reaching lower impedances than commercial MEAs and comparable to state-of-the art spin-coated polymers. With the presented preliminary biocompatibility tests, we aim to show that unconventional deposition processes can access to wider ranges of materials’ chemistry and morphology that cope with biological-constraints/electrical-performances to unlock future emerging bioelectronics technologies.

R.P.43
18:00
Authors : Miguel A. Jimenez-Munoz, Kenny Malpartida-Cardenas, Nicolas Moser, Pantelis Georgiou, Jesus Rodriguez-Manzano
Affiliations : Centre for Bio-Inspired Technology, Department of Electrical and Electronic Engineering, Imperial College London, London, UK

Resume : According to the WHO, Malaria is one of the most severe infectious diseases in the world, affecting almost half of the population. Thus, early diagnosis becomes critical. This disease is caused by the Plasmodium parasite whose main human-infective species are: P. Falciparum (Pf), P. Vivax (Pv), P. Ovale (Po), P. Knowlesi (Pk) and P. Malariae (Pm). Point-of-Care (PoC) systems promise to be a fast and costless alternative to current diagnosis methods, suitable for resource limited settings. Nevertheless, they still present some limitations such as low detection limit or the need of expensive optical equipment. More importantly, most of these PoC systems only focus on the detection of pan-Plasmodium, or Pf, leaving infections caused by other species undiagnosed. Increasing infections of Pv, Pk or Po in countries such as India or Malaysia have been recently reported, urging the development of new PoC devices for their detection. In previous work we have shown a novel Lab-on-a-Chip (LoC) electrochemical biosensor based on real-time LAMP and Ion-Sensitive Field Electrode transistors (ISFET) able to detect Pf. In this work, we propose to extend this system to the detection of other Plasmodium species. This methodology allow us to combine the accuracy of nucleic acid amplification with sensitive and unlabeled detection provided by the ISFET sensors. Furthermore, this LoC technology is promising for multiplexing, allowing the detection of several pathogens at once, ideal for PoC applications.

R.P.44
18:00
Authors : M. Chetyrkina (1), F. Talalaev (1,2), S. Kostyuk (3) and P. Troshin (1,2).
Affiliations : (1) Skolkovo Institute of Science and Technology, Russia (2) Institute for Problems of Chemical Physics of Russian Academy of Sciences (IPCP RAS), Russia (3) Research Centre for Medical Genetics (RCMG), Russia

Resume : The preventive and personalized medicine strategy for disease treatment stands apart from traditional medicine therapy. Nowadays, non-invasive monitoring and diagnostic systems based on wearable electronics and e-skin are considered as crucially important for implementation of smart healthcare technologies. However, materials for such devices should fulfill specific requirements, e.g. have a good combination of charge-transport properties, mechanical flexibility, and biocompatibility. In this work, we explored the biological effects of a series of different organic semiconductors on in vitro living system. Human embryo lung fibroblasts were incubated at standard conditions with thin films of studied materials. Cells were fixed at different time points in order to find out acute and long-term effects on fibroblasts (30 hours, 96 hours, 14 days). Flow cytometry and immunofluorescence microscopy techniques were performed with antibodies to H2A histone family member X and 8-oxoguanine for DNA damages evaluation. The occurred changes in cell metabolism were detected by real-time polymerase chain reaction, which was carried out with primers to the genes involved in apoptosis regulation and oxidative stress in cells. Results of our study demonstrated that some of the studied organic semiconductors represent biocompatible materials suitable for wearableand on-skin electronics.

R.P.45
18:00
Authors : Boyuan Hao, Pavani Cherukupally, Daryl Williams*
Affiliations : Surface and Particle Engineering Laboratory, Department of Chemical Engineering, Imperial College London, SW7 2AZ

Resume : Water scarcity is a large global challenge threatening approximately four billion people. Ambient air contains 13000 trillion liters of water vapor equivalent to approximately 10% of all freshwater in all the world lakes. It can serve as an alternative water resource for addressing the water crisis. Some materials can be used to extract water from the atmosphere, but they need either an expensive water collection device or high temperature to achieve water vapor desorption, which are both costly and labor intensive. The objective is to develop a material that can be used to harvest water from the air in a cost and energy efficient manner. Superabsorbent polymers (SAPs) that have water uptake capacity of 200 to 500 times of their own weight of liquid water have not been investigated for moisture harvesting. Herein, inspired by desert beetles and cactus plants, we investigated the efficacy of SAPs, sponges coated with PNIPAAM cones to sorb water vapor at relative humidity from 30 to 90%, as commonly found in ambient atmospheric conditions. A state-of-the-art Dynamic Vapor Sorption was used to evaluate the kinetics and diffusion properties of water vapor at a relative humidity of 30 to 90% and temperatures of 10 to 50°C. The kinetics of moisture uptake will be also investigated for various humidified airflow rates. The kinetics and diffusion properties of the SAPs will then be quantified using physics-based models.

R.P.46
18:00
Authors : Zhuyun Li, Benhui Hu, Eugene V. Makeyev, Xiaodong Chen
Affiliations : Dr. Z. Li, Dr. B. Hu, Prof. X. Chen Innovative Center for Flexible Devices (iFLEX) School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798, Singapore Prof. E. V. Makeyev Centre for Developmental Neurobiology King’s College London New Hunt’s House London SE1 1UL, UK

Resume : Cellular signalling through diffusible factors and direct cell-to-cell contacts are known to play a critical role in controlling cell viability. To understand how mechanical neuron-neuron contact affects neural survival is essential for study brain development. However, tools to systematically manipulate inter-neuron connection rarely occurs in previous studies. Herein, we developed a strategy to impose stereotypic interneuronal spacing and connectivity using topology micropatterns. With the artificial surface, we found that increased inter-neuron connectivity promotes the survival of neurons by activating neuron survival pathways. Overall, our approach helped to pave the way to improve primary neuron cell culture methodology, and more importantly, enable us to manipulate neuronal survival simply by controlling the connectivity between neurons.

R.P.47
18:00
Authors : Ting-Ying Wu, Zhen-Yu Guan, Chih-Yu Wu and Hsien-Yeh Chen*
Affiliations : Department of Chemical Engineering, National Taiwan University

Resume : The 3-dimensional(3D) porous materials or scaffold have been widely applied to the different fields. Here, we report an innovative manufacturing process for the fabrication of 3D porous structures containing any ingredients even the living cells via the chemical vapor deposition (CVD). This report indicates that the scaffold via sublimation/deposition process could encapsulate any substances, including living cells, growth factors and functional guiding molecules, to form a mono- or multi-functional scaffold and performed its biological function. According to the results, the survival rate of the cell-loaded scaffold via sublimation/deposition process was 80.8±5.3%. The proliferation and osteogenesis activities of MC3T3-E1 loaded in scaffold composited with ingredients, fibroblast growth factor (FGF-2) and bone morphogenetic protein (BMP-2), respectively, could be enhanced, comparing with cell-loaded scaffold without ingredients. Otherwise, osteogenesis and adipogenesis of human adipose-derived stem cells (hASCs) encapsulated in scaffold composited with PRP and cultured with induction medium performed better than the others group without PRP or induction medium. Moreover, according to the neurogenesis test, neurite length of pheochromocytoma 12 cell line (PC12) loaded in scaffold composited with ingredients, PEDOT:PSS and PRP, was 115.3 ± 26.8 μm which is the longest in different groups and tendency of neurogenesis activity were also as same as neurite length. Based on these result, the ingredients could be successfully loaded in the same Trojan scaffold, and also perform their individual functions. This novel approach and scaffold mentioned here provide another way for fabricating multifunctional scaffold with potentially broad applicability.

R.P.48
18:00
Authors : Dongkyu Kang * (1), (2), Jaechul Pyun (2) & Joonseok Lee (1), (3).
Affiliations : (1) Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, South Korea (2) Department of Material Science and Engineering, Yonsei University, Seodaemun-gu Seoul 120-749, South Korea (3) Division of Nano and Information Technology, KIST School, Korea University of Science and Technology (UST), Seoul * lead presenter

Resume : Upconversion nanoparticles (UCNPs) are commonly used as energy donor via a LRET (luminescence resonance energy transfer) in biological applications. In contrast to classic RET donors such as gold nanoparticles, quantum dots, UCNPs can emit near-infrared (NIR) upon the NIR excitation, which provides reduced signal-to-noise ratio due to the strong penetration and less autofluorescence in NIR region called diagnostic window. Here, we report NIR-to-NIR signal based LRET system for detection of progesterone, a natural steroid female sex hormone. We synthesized NaYF4@NaYF4:Yb,Tm@NaYF4 inert-core/active-shell/inert-shell UCNPs as LRET donor. Progesterone-HRP-IRdye and progesterone-BSA-IRdye were used as LRET acceptors with different size for efficient LRET from the UCNPs due to exact spectrum match of the UCNP’s emission and IRdye QC-1’s absorption. Anti-progesterone antibody was conjugated on the surface of water-soluble UCNPs. Progesterone was detected in NIR-to-NIR signal based LRET system via competitive immunoassay. The limitation of detection for progesterone was determined to be 1.36 pg/ml in 10-fold diluted human serum samples. We believe that the LRET system have considerable diagnostic potential, being background-free and able to rapidly detect biomarkers.

R.P.49
18:00
Authors : Pingqiang Cai, Zhuyun Li, Ela Sachyani Keneth, Luying Wang, Changjin Wan, Ying Jiang, Benhui Hu, Yun-Long Wu, Shutao Wang, Chwee Teck Lim, Eugene V. Makeyev, Shlomo Magdassi,* and Xiaodong Chen*
Affiliations : Prof. X. Chen, Dr. P. Cai, Dr. Z. Li, Dr. C. Wan, Dr. B. Hu, Dr. Y.-L. Wu, Y. Jiang Innovative Center for Flexible Devices (iFLEX),School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore Prof. S. Magdassi, E. S. Keneth Institute of Chemistry, Centre for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. Prof. S. Wang, Dr. L. Wang CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing, 100190, P. R. China. Prof. C. T. Lim Mechanobiology Institute, Department of Biomedical Engineering, National University of Singapore, 5A Engineering Drive 1, Singapore 117411. Prof. E. V. Makeyev Centre for Developmental Neurobiology, King's College London, New Hunt's House, London SE1 1UL, UK.

Resume : Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems led current studies to approximate it simply as an in-plane stress. To evaluate possible contribution of force vectors acting in other directions, we reconstituted double epithelium in a 3D-printed GeminiChip containing a sessile and a pendant channel. Intriguingly, the sessile epithelia were prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favored live cell delamination. Transcriptome analyses showed upregulation of RhoA, BMP2 and hypoxia signaling genes in the pendant epithelium, consistent with the onset of an epithelial-mesenchymal transition program. HepG2 microtumor spheroids also displayed differential spreading patterns in the sessile and pendant configuration. Using multilayered GeminiChip, our results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer.

R.P.50
18:00
Authors : Jing Yu, Chen Xiaodong
Affiliations : Nanyang Technological University

Resume : Biological processes such as morphogenesis, angiogenesis or embryogenesis involves group of cells coordinating their movements collectively to achieve specific functions, in a constricted fashion. Unlike single cell, collective cellular motions, which involve intercellular coordination, are much more complex and largely remain mysterious. Variances in environment could easily lead to changes in cell behaviors; factors such as chemical, physical or mechanical signals are common regulators in guiding cell migration. Among these factors, topological cue is a critical, yet easily neglected factor. Different from most benchside scenarios, in which migrations are allowed on infinite surfaces, the physiological microenvironment of cellular migration is usually confined. Here we built fibronectin patterns on polyacrylamide substrate to study confined cell migration. We showed that motion of cell cohort could be modeled through topological cues and confinement. Madin-Darby Canine Kidney (MDCK) cell line is used as an epithelial model to describe a form of oscillation phenomenon under various circular confinement, which amplitude and frequency vary with both the size of constrain as well as regions in the confined monolayer. Through spatio-temporal force analysis, increased intercellular tension and cell-substrate traction are observed with increasing degree of confinement. Together with confocal microscopy of actin cytoskeleton and average orientation field mapping on the cell sheet, a possible fluid-to-solid transition could attribute to this interesting collective cell motion.

R.P.51
18:00
Authors : Eunyoung Jeon(1)*, Joonseok Lee(1)
Affiliations : (1) Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Division of Nano and Information Technology, KIST School, University of Science and Technology (UST), Seoul, 02792, Korea

Resume : Conventional drug-eluting stents (DESs), biomedical implants, mainly use polymers or laser etching to load drugs. However, there are limitations in that polymers cause side effects such as late-thrombosis in the body and laser etching is complicated and expensive. Herein, we propose a porous nanostructured silica film for polymer-free DESs platform, which is synthesized by a sol-gel process using a catalyst, a silicon source, and structure-directing agents. As the structure-directing agents form micelles and grow on the surface, the porous nanostructured silica film is formed and has tunable pore sizes and channel lengths by adjusting reagents, molar ratios and reaction time. This suggests that a large amount of materials can be loaded, from large molecular weight molecules to small ones, and the films can be easily formed on the surface of commercial bare-metal stent. In addition, it is expected to be physically stable in the actual applications through pico-indentation experiment, expansion simulation and SEM, which evaluates the physical characteristics such as young's modulus (85.26 GPa) and hardness (2.69 GPa). Moreover, surface functionalization into hydrophobic with silane coupling agents (Octyltrimethoxysilane, Hexadecyltrimethoxysilane) allow the hydrophobic drugs to be loaded without polymers. The porous nanostructured silica film may represent an attractive platform that can be used in many other areas as well as polymer-free DESs.

R.P.52
18:00
Authors : Petruta Preda1,3*, Marioara Avram1,2, Diana Stan2, Bogdan Mincu2, Anton Ficai3
Affiliations : 1 National Institute for Research and Development in Microtechnologies - IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190, Bucharest, Romania 2 DDS Diagnostic SRL, 7 Vlad Judetul Street, Bucharest, Romania 3 Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 1-7 Gh. Polizu, 011061, Bucharest, Romania

Resume : Bionanocomposite films based of polycaprolactone (PCL) and fish collagen nanoparticles embedded with sea buckthorn oil were prepared by the solvent evaporation method at room temperature conditions. These materials were performed in order to be used as promising candidates in medical applications like regeneration of skin wounds and not only (surgical suture and prosthetic devices) by improvement the proliferation of fibroblast cells properties. The following raw materials were used for obtaining the polymeric composites: polycaprolactone (Sigma-Aldrich, Mw = 45,000); fish collagen extracted in the laboratory from fish waste; pure cyclohexanone (Sigma-Aldrich); acetic acid (Sigma-Aldrich) and sea buckthorn oil. Both polymeric films developed as well as collagen and sea buckthorn oil respectively have been physico-chemically and biologically characterized (FT-IR, SEM, UV-vis, EDX, HPLC, SDS–PAGE, WCA/water contact angle and fibroblast cell cultures study). Experimental results suggest that polycaprolactone films with collagen and sea buckthorn could be used for medical applications, when additional studies are required. Keywords: polymeric composites, skin wounds, fibroblast cell cultures ACKNOWLEDGEMENTS The work of PETRUTA PREDA has been funded by the Operational Programme Human Capital of the Ministry of European Funds through the Financial Agreement 51668/09.07.2019, SMIS code 124705. This work was supported by the grant COP A 1.2.3., ID: P_40_197/2016.

R.P.53
18:00
Authors : Jin Gu Kang, N. Ashwin K. Bharadwaj, Gaurav Chaudhary, Ashesh Ghosh, Marta C.Hatzell, Kenneth S. Schweizer, Randy H. Ewoldt, Paul V. Braun
Affiliations : Korea Institute of Science and Technology; University of Illinois at Urbana-Champaign

Resume : The study of a composite system made of semi-flexible polymeric networks combined with colloidal particles has remained in its infancy despite its ubiquitous nature in the biological space. By imparting functionalities into colloids or polymer networks, reconfigurable and active materials system that dynamically respond to external stimuli can be realized. Here, we demonstrate the 3D hydrogel composites consisting of semi-flexible fibrin networks and colloids. We develop the methods of integration of colloidal particles into fibrin networks and observe the structure of the resulting composite hydrogels. We characterize the mechanical properties by measuring oscillatory shear elastic moduli at the linear and non-linear regimes. The relation between the structure and the mechanical properties are discussed. Furthermore, we synthesize thermo-responsive hard-sphere colloids and soft-microgel colloids via atom-transfer-radical-polymerization and surfactant-free emulsion polymerization respectively. We successfully incorporate these into fibrin networks using the developed method. The changes in structure and dynamics upon the temperature change are observed and the origin of this switchable property is discussed.

R.P.54
18:00
Authors : A.F. Bonciu, S. Iosub, M. Filipescu, V. Dinca* and M. Dinescu
Affiliations : A.F. Bonciu 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania 3) University of Bucharest, Faculty of Physics, RO 077125, Magurele, Romania S. Iosub 2) Institute of Biochemistry of the Romanian Academy, 060031 Bucharest, Romania M. Filipescu 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania V. Dinca 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania M. Dinescu 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania

Resume : In the last few years, Ceria (CeO2) nanostructures are thought to improve the biointerface mechanical properties, but also to stimulate the regenerating tissue biochemical activity by neutralizing the oxidative stress and stimulating cells proliferation. Nevertheless, there is a lack of information of mamallian cells on Ceria coatings, especially with well-defined nano and microstructured surface. Within this context, we present for the first time new designed pyramid shaped nanostructured ceria films obtained by Pulsed Laser Deposition (PLD), targeting the modulated response of SaOs-2 bone cells. The nanostructured surfaces obtained by PLD revealed shapes from the quasipyramidal, with rounded edges and dimensions of 90-120 nm, to the sharp edges and dimensions up to 350 nm, obtained by varying the number of pulses. The shapes and sizes of the nanostructures influenced both wettability and cellular behaviour. Nevertheless, the influence of pyramidal shaped ceria on the in vitro biological performance of SaOs-2 cells used as bone cell model was demonstrated by the differences in early adhesion and distribution of phenotype SaOs-2 cells, onto the ceria surface. In conclusion, different nanostructured ceria films obtained by PLD influenced especially the early response of osteosarcoma cell and are good candidates to improve the performance of orthopedic implants. In perspective, different cells lines response will be analysed toward establishing an osteogenic response. Acknowledgement This work was supported by the National Authority for Research and Innovation in the frame of the Nucleus Program and grants of the Romanian Ministry of Research and Innovation,CCCDI-UEFISCDI, project numbers PN-III-P1-1.2-PCCDI-2017- 0637 (MultiMonD2).

R.P.55
18:00
Authors : Ivanyuta O.,1 Gogotsi H.,1 Buzaneva E.,1 Ritter U.,2 Scharff P.,2
Affiliations : 1 National Taras Shevchenko University of Kyiv, Ukraine; 2 TU of Ilmenau, Institute for Chemistry and Biotechnology, Germany E-mail: oivanuta@gmail.com

Resume : The characterization of the protein amino-acids in natural cells and mimetic medias is one of great value in the amino-acid studies. Using results of investigations for intermolecular interaction between the C60 and ds-, ss- DNA sequences [1], predictive modeling of a interface structures for the fullerol (immobilized by –OH groups fullerene C60) molecules in a cluster and this molecule at an interface with metal ion (Cu or Ag ) complex having coordinative bond to selected amino-acid (glycine, alanine, methionine, histidine) molecules [2,3] has been realized. This modeling should derive interface properties from the chemical functionalities of the constituents, while operating stimuli- response molecular nanostructures in aqueous solutions with amino-acid molecules. Modeling was carried out taking in account that these interfaces organization may increase the photoactivity and the biocompatibility to this carbon molecule counterpart. The interface designing concept as the base for the fullerol molecule in a cluster sensor and molecular ion metal complex sensor development has been discussed. Based on well established rationale of interface organization in biological macromolecules (nucleic acids, proteins), driven by non-covalent interactions (hydrogen bonding, hydrophobic effect and electrostatic interaction) in living cells, we have plan to select biomolecules for non-covalent interaction with a core of the fullerol molecule and a molecule of organic ligand in ion metal complex – amino acids. Then molecular interface organization in solutions can be revealed by photospectroscopy. From experimental results specific interface formation between [Сuх(5-amtetrazole)у]2 complex and histidine molecule for organic specially synthesis ligand with the same coordination nature of this amino-acid molecule has been determined. A special structure of a ligand and histidine molecules gives more coordination geometry to molecular complexes, determines specific interface formation in this supramolecule.Selective coordination of such supramolecule in solutions with the histidine molecules (pH 2.7 – 12.9) was confirmed by absorbance spectra with the intensive band (600 800 nm) and it is assigned to the d – d transition of Cu. Maximum position shifted from 753 to 615 nm after adding the histidine was demonstrated. The histidine concentration effect for the solutions with the fullerol molecular clusters and the functionalized by Cu –azole ligand complex fullerol molecules was also determined. The next step is the development of controlled by light excitation interface between the fullerol, selected ion metal complex and many protein-acid molecules organization for their photo-spectroscopy recognition and single molecular biosensor controlled design. References: 1. O. Kysil, I.Sporysh, E.Buzaneva, T. Erb, G.Gobsch, U.Ritter, P.Scharff, Mat. Sc. and Eng. B169/1-3 (2010) 85. 2. O. A. Bondar, L. V. Lukashuk, A. B. Lysenko, H. Krautscheid, E. B. Rusanov, A. N. Chernega, K V. Domasevitch, Cryst. Eng. Comm, 10 (2008), 1216. 3. J. G. Mesu; T. Visser; F. Soulimani; E. E. van Faassen; P. de Peinder; A. M. Beale; B. M. Weckhuysen, Inorg. Chem., 45/5 (2006) 1960.

R.P.56
18:00
Authors : Rotar, D.V. (1), Kobasa, I.M. (2), Kornyakov A. O. (3), Vorobets, M.M. (4)
Affiliations : (1) PhD, Departments of Microbiology and Virology Bukovina State Medical University, 2, Theater Square, Chernivtsi, 58002, Ukraine E-mail: diana.rotar@bsmu.edu.ua (2)Yu. Fedkovych Chernivtsi National University., Ukraine 2 Kotsiubynskiy str., Chernivtsi 58012 E-mail: i.kobasa@chnu.edu.ua (3) Bukovyna State Medical University, Ukraine 3rd year student of medical faculty Bukovina State Medical University, 2, Theater Square, Chernivtsi, 58002, Ukraine Email: sahosome@gmail.com (4)Yu. Fedkovych Chernivtsi National University. , Ukraine 2 Kotsiubynskiy str., Chernivtsi 58012 E-mail: m.vorobets@gmail.com

Resume : Antibiotic resistance is one of the most pressing problems of modern medical practice. This situation should be addressed through the rational and correct prescribing antimicrobial agents in clinics. Changes in Ukraine's health care system have the need for reconstruction and restoration of medical facilities. The use of ordinary repair materials for this purpose can not be used. Studies have shown the dependence of adhesive and colonization properties of clinical antibiotic-resistant strains of microorganisms from the effects of materials which based on TiO 2 Degussa P25 with photocatalytic activity under UV with a wavelength of 365 nm with a different exposure time. The experiment was carried out by the method of diffusion, a microorganism lawn culture was sown on a cup with a nutrient medium, a part of the cup was covered with a light-proof material, and placed in a light chamber (the duration of irradiation of the lawn was different) . After 24 hours cultivation of lawn cultures of antibiotic-resistant strains of microorganisms were recorded. Established that material with nano-TiO 2 under UV irradiation him with a wavelength of 365 nm resulted in stunted growth and development of culture lawn clinical strain of microorganism. Thus, from a culture with a microbial load of 10^5 cells / ml on the control samples developed a dense lawn, indicating the regeneration of the culture, its adhesion to the surface and colonization of the surface. Nano-TiO 2 at UV with a wavelength of 365 nm caused a delay in the development of all cultures of clinical strains of microorganisms. Thus, from the working suspension with a microbial load of 10^5 cells / ml grew single colonies in an amount of from 2 to 4 CFU. Materials based on Degussa P25 TiO 2 are promising for the manufacture of surfaces of medical equipment where there is a threat of colonization by antibiotic resistant strains of microorganisms.

R.P.57
18:00
Authors : Gryn D.V., Naumenko A.P., Gubanov V.O.
Affiliations : Taras Shevchenko National University of Kyiv

Resume : Cisplatin (cis-Pt) is a complex of platinum which widely used as an anticancer drug. Transplatin (trans-Pt) has the same chemical formula, but different positions of atoms. Cisplatin connects to DNA in cancer cells and stops DNA replication, namely making cross-links between guanine bases. Transplatin is less reactive to DNA but also demonstrate cytotoxicity effect. In this work we make an attempt to connect the differences in the biological activity of mentioned structures with the data obtained by optical spectroscopy methods. The UV-vis spectra as well as fluorescence and phosphorescence spectra of water solutions of cis-Pt and trans-Pt were investigated at temperatures 300 and 77 K. Quantum chemical calculations were used for correct interpretation of obtained results. We also characterized these compounds using such powerful method as Raman spectroscopy.

R.P.58
18:00
Authors : Alkmini D. Negka, Panagiota Koralli, Maria Goulielmaki, Lida Evmorfia Vagiaki, Aristea Pavlou, Giannis N. Antoniou, Panagiotis E. Keivanidis, Dimitris Moschovas, Apostolos Avgeropoulos, Aristotelis Xenakis, Vassilis Zoumpourlis, Antonia Dimitrakopoulou-Strauss, Vasilis G. Gregoriou, Christos L. Chochos
Affiliations : Clinical Cooperation Unit Nuclear Medicine, German Cancer Research Center, 69120 Heidelberg, Germany;Institute of Chemical Biology, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, Athens, 11635, Greece; Department of Mechanical Engineering and Materials Science and Engineering, Cyprus University of Technology, Limassol 3041, Cyprus;Department of Materials Science Engineering, University of Ioannina, Ioannina 45110, Greece;National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, Athens, 11635, Greece

Resume : To date, the therapeutic properties of π-conjugated polymers especially in the form of aqueous nanoparticles, the so-called conjugated polymer nanoparticles (CPNs), have been verified merely upon laser irradiation via the well-established photodynamic and photothermal therapies. In this contribution, it is manifested for the first time the natural anticancer properties of single CPNs. This has been accomplished by studying the cell viability of new CPNs in physiological human umbilical cord mesenchymal stem cells (WJ-MSC), human colorectal carcinoma cell line (HCT116) and metastatic human melanoma cell line (WM164). The discovery of natural anticancer properties in conjugated polymers can form a new frontier in nano-biotechnology and nanomedicine and represent a new direction that is orthogonal to the more established areas of conjugated polymers for optoelectronic devices and conjugated polyelectrolyte biosensors. The combined natural anticancer and bioimaging properties of CPNs point to a new strategy for obtaining novel organic nanomaterials for cancer theranostics.

R.P.59
18:00
Authors : Panagiota Koralli, Alkmini Negka, Lida Evmorfia Vagiaki, Antonia Dimitrakopoulou-Strauss, Vasilis G. Gregoriou, Christos L. Chochos
Affiliations : National Hellenic Research Foundation, Institute of Chemical Biology, Athens, Greece; German Cancer Research Center (DKFZ), Clinical Cooperation Unit Nuclear Medicin, Heidelberg, Germany; National Hellenic Research Foundation, Athens, Greece

Resume : Low-bandgap aqueous conjugated polymer nanoparticles (CPNs) have been prepared and applied in biological applications, especially in bio-imaging, due to their unique optoelectronic properties.In this study, we successfully synthesized a series of conjugated polymers combining thiophene as the electron donating and quinoxaline as the electron deficient (TQs) with varying the number of fluoro atoms in the repeat unit. Water-soluble nanoparticles formatted by two different methods (nanoprecipitation and encapsulation), supporting their potential use as low-bandgap fluorescent probes for bio-imaging.The DLS measurements indicate that all CPNs in aqueous suspension have a unimodal size distribution less than 80nm.TEM & SEM measurements showed that the CPNs have spherical shape.The optical properties of the CPNs show light absorption and fluorescence emission in the UV-Vis region,tunable depending on the structure, the position and the number of fluoro atoms on the polymer backbone.It is revealed a significant reduction of the fluorescence quantum yield in aqueous media derived from severe aggregation and charge transfer induced fluorescence quenching, which can be controlled with proper rational design on the polymer backbone. Moreover, the on-going tests for cellular cytotoxicity and the ability of the obtained probes to image cancer cells are of great importance.Overall, CP dots introduce a new generation of fluorescent probes that appear to be the quintessential contrast agents

R.P.60
18:00
Authors : H.Gogotsi(a),,O.Ivanyuta(a),,E.Buzaneva(a),U.Ritter(b),P.Scharff(b)
Affiliations : (a)National Taras Shevchenko University of Kyiv,, 64, Volodymirska Str., 01601 Kyiv, Ukraine, oivanuta@gmail.com (b)TU Ilmenau, Institut für Chemie and Biotechnology, Postfach 100565, 98684 Ilmenau, Germany.

Resume : The design and optical characterization of the protein amino - acids is key for understanding of the amino-acids molecules behaviour as basis sequences of biological macromolecules ( proteins) and their using as photoactive molecules, for example, in an advanced photovoltaic systems for an energy sources [1]. Therefore, we have start to design amino-acids molecules and study classic models for light excited electron transition through LUMO-HOMO band gap for the molecules and discuss ones using theories for LUMO-HOMO band gap formation for these molecules having different confirmations [2]. Then we selected amino-acid molecule for studies by the PL spectroscopy take in account that can use the laser with 408 nm wavelength (3.3 eV) for experiments. The cysteine PL excited by the laser with wavelength 408 nm( 3.3 eV) is characterized by the band in 1.5- 2.7 eV range. This band included of subbands, which are centered at 2.04, 2.24 and 2.4 eV. The present of subbands are explained by the dependence on the conformation of the molecule geometry in the cysteine layer of the HOMO-LUMO value as it is found due to compare of these subbands with calculations of electronic excitations of the cysteine conformers [2]. Therefore it is possible to use light excited electron transitions in the cysteine layer with different molecule geometry in photonic systems. 1. Koo, Suk Tai Chang, Joseph M. Slocik, Rajesh R. Naik and Orlin D. Velev, Aqueous soft matter based photovoltaic devices, J. Mater. Chem., 2011, Advanced Article.http://pubs.rsc.org/en/content/ArticleLanding/2011/JM/COJMO1820A#divAbstract;2.R. Maul, M. Preuss, F. Ortmann, K. Hannewald, and F. Bechstedt, Electronic Excitations of Glycine, Alanine, and Cysteine Conformers from First-Principles Calculations, J. Phys. Chem. A 2007, 111, p.4370-4377.

R.P.61
18:00
Authors : Krasimir Koev*, Nikolaj Donkov, Hristo Naidenski, Vesslin Kussovski, Timerfayaz Nurgaliev, Latchezar Avramov
Affiliations : Assoc. prof. M.D. PhD Kr. Koev* Department of ophthalmology, Medical University 8 Byalo More str., Sofia, Bulgaria, E-mail: k00007@abv.bg Assoc. prof. M.D. PhD Kr. Koev, Assoc. Prof. Dr. N. Donkov, Prof. D.Sc. T. Nurgaliev, Prof. D.Sc. LatchezarAvramov Institute of Electronics “Acad. Emil Djakov”, Bulgarian Academy of Sciences 72 Tsarigradsko chaussee blvd., 1784 Sofia, Bulgaria Prof. DVM, D.Sc. Hristo Najdenski, Assoc. prof. M.D. PhD Vesslin Kussovski The Stefan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 GeorgiBonchev str., 1113 Sofia, Bulgaria

Resume : Long-term use of ocular prostheses causes frequent ocular infections with a number of adverse effects.The antibacterial and antifungal activity was investigated of Ag-doped Al2O3 nanolayers deposited by radioactive magnetron sputtering on ocular prostheses after a two-year period. A microbiological study was performed to determine the antibacterial action of the nanocomposite Ag/Al2O3 nanolayers against gram positive and gram negative bacteria, and an antifungal action against Candida albicans. From the conducted microbiological studies we have found the preservation of the antibacterial and antifungal action of the Ag/Al2O3 nanocoating of the eye prostheses after two years. The results of microbiological studies show that antibacterial and antifungal activity has the same full inactivation by intensity for the reference period. Experimental studies show a very promising application of such antibacterial and antifungal Ag/Al2O3 nanocoating to reduce ocular infections in the placement of ocular prostheses over a prolonged period of use. Keywords: Ag/Al2O3 nanolayers, antibacterial and antifungal action, ocular prostheses coating, two-year period

R.P.62
18:00
Authors : Ahmaduddin Khan, Niroj Kumar Sahu
Affiliations : Centre for Nanotechnology Research

Resume : Nanoparticles mediated drug delivery system has immense therapeutic applications especially in the field of cancer treatment. The chemo-therapeutic efficacy is enhanced when combined with radiation therapy or thermal therapy (Hyperthermia). For thermalchemotherapy, appropriate functionalised magnetic nanoparticles with desired magnetic parameter are pivotal. In this direction, we synthesised a highly aqueous dispersible PEG-dicarboxylic acid functionalised iron oxide (Fe3O4) nanocluster with superior magnetic properties by a simple solvothermal approach. The well distributed nanocluster (size~200nm) are formed by the assembly of smaller nanoparticles. Fe3O4 crystallizes in inverse spinel structure with crystallite size of 16.9 nm and possess mesoporous structure with high specific surface area of 71.3 m²/g. The carboxyl group was activated using hydrazine hydrate to form pH sensitive hydrazone bond which further was utilised to attach with doxorubicin (DOX) through C=O linkage. High drug loading efficacy was achieved due mesoporous nature of the nanoparticles and carboxyl group activation. This system shows higher and sustained release at pH 4.3 in comparison to normal physiological pH of 7.4 because of labile hydrazone bond. The synthesised Fe3O4 nanoclusters are biocompatible upto a concentration of 1mg as observed in vitro in MCF-7 breast cancer cell line. Due to higher saturation magnetisation of the nanocluster, a hyperthermic temperature of ~ 43 ºC was achieved in just 105 s at nanoparticle concentration of 2 mg/ml and at the applied AC magnetic field strength of 35.2 kA/m and frequency of 316 kHz. Hence, the PEGylated mesoporous Fe3O4 nanoclusters can be effectively utilised for thermochemotherapy of cancer.

R.P.63
18:00
Authors : Jooran Kim
Affiliations : Korea Institute of Industrial Technology

Resume : 3D bioprinting is a widely used technology to dispense cell-laden biomaterials for rapid fabrication of complex 3D tissue constructs or artificial organs. To date, many studies have been investigated the deposition and patterning of cell-laden bioinks with a 3D bioprinter. However, the precise positioning, reasonable mechanical properties, and controlled cell distributions of constructs in 3D bioprinting system still remain technical challenges. In this study, we developed heterogeneous cell-laden microchannel network using human umbilical vein endothelial cell-cardiomyocytes by a direct patterning with a 3D bioprinter. We fabricated 3D tissue constructs consisting of the core (human umbilical vein endothelial cell -laden collagen) and the sheath (cardiomyocytes-laden gelatin methacrylate). We could achieve a stable 3D multilayered core-sheath structure with the reasonable elastic modulus. This system also facilitated cell alignment and migration within each constructs and promoted vascular network with high cell viability. Calcium imaging was used to optically probe intracellular calcium ion signals during excitation-contraction coupling in cardiomyocytes within 3D vascular network. This paper presents the new approach for fabricating vascularized heterogeneous 3D scaffolds and highly controlled deposition technique of bioinks. The cell-laden 3D constructs could be extended to serve as in vitro models for clinical cardiovascular disease researches and cardiovascular tissue regenerations.

R.P.64
18:00
Authors : A.I. Visan1*, I. Jinga1, I. Ungureanu1, F. Antohe2, M.C. Chifiriuc3, R. Cristescu1
Affiliations : 1 Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, Magurele, Ilfov, Romania; 2 Proteomics Department, Institute of Cellular Biology and Pathology “N. Simionescu” Romanian Academy, Bucharest, Romania; 3 Department of Microbiology, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania *Corresponding author: anita.visan@inflpr.ro

Resume : Polymer-coated stents with antiproliferative drugs and growth factor have been proposed to defeat adverse reactions which occur in cardiovascular treatment. We report on successful deposition of functionalized thin films of (everolimus and paclitaxel) drugs encapsulated in complex matrices [either blends of poly(L-lactide) and collagen or nanoparticles of poly(lactic-co-glycolic acid) - polyvinyl alcohol] containing vascular endothelial growth factor (VEGF) by Matrix Assisted Pulsed Laser Evaporation (MAPLE). The morphology, hydrophilicity, and biodegradability of the composite coatings have been investigated. The main recipes of the drug functionalized polymer matrices, synthesized by MAPLE, have been validated by biological investigations, drug release profiles, and physical-chemical investigations. In vitro evaluation tests performed on the fabricated thin films have revealed great biocompatibility, that may endorse them as competitive candidates for the development of improved non-toxic surfaces resistant to microbial colonization. The proposed functionalized thin films apart from preventing the restenosis (due to selected drug inhibitors), also could eliminate the risk of late thrombosis (as the covered stent is replaced by connective tissue thanks to VEGF addition) and are expected to act as improved/appropriate/effective coatings for the next-generation drug-eluting stents.

R.P.65
18:00
Authors : Anita Visan1, Carmen Ristoscu1, Gianina Popescu-Pelin1, Mihai Soprony1, R. Cristescu1, Carmen Mariana Chifiriuc3, David Grossin4, Fabien Brouillet4, Ion N. Mihailescu1
Affiliations : 1 National Institute for Lasers, Plasma and Radiation Physics, Magurele, Ilfov, Romania ; 2 National Institute of Materials Physics, Magurele, Ilfov, Romania;3Department of Microbiology, Faculty of Biology, University of Bucharest, 060101; Research Institute of the University of Bucharest –ICUB, Spl. Independentei 91-95, Bucharest, Romania;4 CIRIMAT – Carnot Institute, University of Toulouse, ENSIACET; 4 Allée Emile Monso, 31030 Toulouse Cedex 4, France

Resume : We report on the successful deposition of antimicrobial chitosan-biomimetic nanocrystalline apatite –tetracycline thin films by Matrix Assisted Pulsed Laser Evaporation (MAPLE) using a KrF* excimer laser source (λ = 248 nm, ζFWHM ≤ 25 ns). Typical FTIR spectra of the obtained thin films were found to be highly similar to the spectrum of the initial powders. Scanning electron microscopy have evidenced a typical morphology characteristic to the deposition technique, advantageous for medical application, the nanoscale roughness increasing with the chitosan concentration. We have evaluated the antibacterial properties of the thin films containing chitosan and tetracycline deposited by MAPLE on titanium samples, using as model organisms both the Gram-negative E. coli and Gram-positive E. faecalis. The biocompatibility of the obtained films deposited on Ti substrates was evaluated by in vitro tests on human bone osteosarcoma cells. These tests have revealed the morphology and cellular cycle of the cells growing on the obtained thin films. The results have demonstrated that the chitosan- biomimetic nanocrystalline apatite-tetracycline composite thin films have improved the bone formation and further facilitated the anchorage between the bone and prosthesis, thus validating the MAPLE efficiency.

R.P.66
18:00
Authors : Iole Venditti 1, Chiara Battocchio 1, Luca Tortora 1, Giovanna Iucci 1, Martina Marsotto 1, Marina Porchia 2, Francesco Tisato 2, Maura Pellei 3, Carlo Santini 3 Corresponding Author, e-mail: iole.venditti@uniroma3.it
Affiliations : 1 Sciences Dept. Roma Tre University of Rome, Italy 2 ICMATE, National Research Council (CNR), Padua, Italy 3 School of Science and Technology, Chemistry Division, University of Camerino, Italy

Resume : Gold nanorods (AuNRs) are successfully employed in drug delivery, biosensors, and biotechnologies [1,2]. Their wide success is due to their unique chemical properties, biocompatibility, easy, cheap and versatile synthesis. In this framework, hydropilic AuNRs were synthetized with the aim to obtain strongly hydrophilic nanomaterials, suitable for drug delivery. AuNRs were investigated by UV-Vis-NIR, FT-IR and HR-XPS spectrocopies, confirming nanosize, with typical surface plasmon resonance (SPR) bands at 550 nm and 900 nm, and surface functionalization by ascorbic acid (AA) and cetyl trimethyl ammonium bromide (CTAB). Therefore, AuNRs were used as drug delivery system for copper (I)-based anti-tumor complex namely [Cu(PTA)4]+[BF4]- (PTA = 1,3,5-triaza-7-phosphadamantane) [3-5]. The loading procedures and efficiency of Cu(I) complexes on the AuNRs surface were optimized in order to control their bioavailability and release. References [1] Park K., Drummy L. F., Wadams R. C., Koerner H., Nepal D., Fabris L., Vaia R. A. Growth Mechanism of Gold Nanorods. Chem. Mater. 2013; 25: 555-563 [2] Venditti I. Engineered gold-based nanomaterials: morphologies and functionalities in biomedical applications. A mini review. Bioengineering. 2019; 6(2): 53 [3] Gandin V., Tisato F., Dolmella A., Pellei M., Santini C., Giorgetti M., Marzano C., Porchia M. In Vitro and in Vivo Anticancer Activity of Copper(I) Complexes with Homoscorpionate Tridentate Tris(pyrazolyl)borate and Auxiliary Monodentate Phosphine Ligands. J. Med. Chem. 2014, 57 (11): 4745-4760. [4] M. Porchia, F. Benetollo, F. Refosco, F. Tisato, C. Marzano, V. Gandin. Synthesis and structural characterization of copper(I) complexes bearing N-methyl-1,3,5-triaza-7-phosphaadamantane (mPTA): Cytotoxic activity evaluation of a series of water soluble Cu(I) derivatives containing PTA, PTAH and mPTA ligands. J. Inorg. Biochem. 2009; 103 (12): 1644 [5] Fratoddi I., Venditti I., Battocchio C., Carlini L., Porchia M., Tisato F., Bondino F., Magnano E., Pellei M., Santini C. Highly hydrophilic gold nanoparticles as carrier for anticancer copper(I) complexes: loading and release studies for biomedical applications. Nanomaterials. 2019; 9: 772 Acknowledgements: The Grant of Excellence Departments, MIUR (ARTICOLO 1, COMMI 314 – 337 LEGGE 232/2016), is gratefully acknowledged by authors of Roma Tre University.

R.P.67
18:00
Authors : Iole Venditti1, Irene Schiesaro1, Martina Marsotto1, Maura Pellei2, Carlo Santini2, Luca Bagnarelli2, Giovanna Iucci1, Carlo Meneghini1, Chiara Battocchio1
Affiliations : 1 Dept. of Science, University of Roma Tre, Viale G. Marconi 446, Rome, Italy. Irene.schiesaro@uniroma3.it 2 School of Science and Technology, Chemistry Division, University of Camerino, Camerino (MC), Italy.

Resume : Copper complexes are coming out as metal-based drugs candidates for the treatment of cancer, due to their wide structural variability, biologically accessible redox properties and bioavailability. Recently, in our quest to find suitable ligands in the design of copper-based anticancer agents, we focused our attention on the synthesis of copper complexes of bis(azol-1-yl)carboxylate ligands functionalized with biomolecules. In addition, Cu(II) complexes of alkyl bis(pyrazol-1-yl)acetate ligands have been investigated for the development of a new and more efficient promoter for the Kharasch-Sosnovsky reaction to oxidize alkenes in allyl position. Since such coordination compounds have low solubility in aqueous medium, it is necessary to design a strategic approach allowing for drug delivery. By conjugating the copper complexes with hydrophilic gold nanoparticles, it is possible to improve their solubility and stability in water, increasing their bioavailability. Moreover, these drug delivery systems allow the investigation of a slow and controlled release of copper complexes [1]. In this context, we carried out a spectroscopic investigation of the molecular, electronic structure and coordination geometry of a selection of Cu(II)-coordination compounds, by means of complementary X-ray techniques induced by Synchrotron Radiation: the molecular and electronic structure were probed by means of SR-XPS and NEXAFS, the oxidation state and the local coordination chemistry of the metal ion were assessed by Cu K-edge XAFS analysed in the near edge (XANES) and extended (EXAFS) regions.

R.P.68
18:00
Authors : Su-Geun Yang
Affiliations : Department of Biomedical Science, College of Medicine, Inha University

Resume : In this study, poly-L-dopa nanoparticle-based drug delivery system was designed for near-infrared light controlled trimodal therapy in which photodynamic therapy (PDT), photothermal therapy (PTT) and chemotherapy are combined to produce synergistic therapeutic effects. Poly-L-dopa nanoparticles (PLD NPs) were synthesized by oxidation and self-polymerization of L-DOPA with KMnO_4. For designing purposes of PTT, PLD NPs were modified with the photosensitizer, pheophorbide a (PheoA), via reducible disulfide linker for glutathione-responsive intracellular release. Due to the ability to convert NIR light into heat, PLD NPs could be used as a photothermal agent without further modification for PTT. Also, PLD NPs have a fluorescence resonance energy transfer- (FRET) based self-quenching effect which reduces the side effects by keeping the system photo-inactive during blood circulation. PheoA modified PLD NPs (PLD-PheoA NPs) were loaded with chemotherapy drug, doxorubicin (Dox), through ᴨ-ᴨ stacking and hydrogen bonding. The in vitro antitumor effect of PLD-PheoA/Dox NPs with trimodal PDT/PTT/chemotherapy was investigated in terms of photothermal efficiency, photoactivity, stimuli-responsive drug release, cellular uptake, and therapeutic efficacy. The best of our knowledge, this is the only study presented in literature, using poly-L-dopa nanoparticles with trimodal synergistic therapy approach for cancer treatment.

R.P.69
18:00
Authors : Ji-Eun Lee, Seung-Min Lee, and Kwang-Ho Lee
Affiliations : Advanced Materials Science and Engineering, College of Engineering, Kangwon National University, Republic of Korea

Resume : To make micropattern including bio-substances on nano-mesh, we developed the photo-crosslinking process using hydrogel. For in-situ micropatterning, the synthesized hyaluronic acid was empolyed with transparent micromold and UV irradiation. The photosensitive hyaluronic acid was rapidly cross-linked through the porous nano-mesh. When hyaluronic acid is patterned on the nanomesh, drugs and cells can be stably selected, and the release of drugs and the behavior of various cells can be controlled according to the patterning conditions. The immobilized material was released by diffusion, which was very precisely controlled through the coating of hyaluronic acid and nanomesh, and the theoretical verification with finite element analysis was performed. The proposed research could be used in the field of tissue engineering for local drug delivery or regeneration of damaged tissues in the body.

R.P.70
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08:45 Plenary Session - (08. 45 – 09.45): Lecture by Prof. André Geim Nobel Laureate in Physics (2010) University of Manchester, U.K.    
09:45 The Nobel Prize in Physics 2010 was awarded jointly to Andre Geim and Konstantin Novoselov “for ground breaking experiments regarding the two-dimensional material graphene”    
09:45 10.00 - Coffee Break    
 
New Frontiers in Smart Carbon SESSION Invited Presenters : Organizer/Chair Prof.Peter Scharff (TU Ilmenau,Germany), Co-Chairs Professors Maurizio Prato (Universita di Trieste, Italy; CICbiomaGune, San Sebastian,Spain), Hsing-An Liu (Shanghai University, Сhina; Nagoya University,Japan), Jun Maruyama (Osaka Research Institute of Industrial Science and Technology,Japan)
10:00
Authors : Prof. Dr. Peter Scharff
Affiliations : TU Ilmenau, Institute of Chemistry and Biotechnology, Weimarer Straße 25 (Curiebau), D-98693 Ilmenau, Germany. peter.scharff@tu-ilmenau.de

Resume : Graphite is composed of layers of linked carbon hexagons. Between the layers, base metals such as potassium and the like, but also metal compounds can be incorporated, forming new substances (GIV). In high-temperature electrolyses any GIV formed may destroy the carbon electrodes. In other processes, such as the production of graphite foils, a versatile material with outstanding chemical and physical properties, make GIV the crucial intermediate. The electrochemical behavior of GIV opened up the possibility of constructing new high-performance lithium-GIV battery systems. New carbon compounds are found and their reaction behaviour characterized [1]. In addition to graphite and diamond, the group of fullerenes is the third carbon form, which has been experimentally accessible by graphite evaporation in the carbon arc. In the case of graphite evaporation, in addition to the fullerenes, similarly constructed carbon tubes. Buckminsterfullerene is soluble in organic solvents and gives a brownish product in the solid state. Fullerenes with many inorganic and organic substances react to form derivatives that have interesting physical properties and potential applications in the field of superconductivity and nonlinear optics. Fullerene research is an area of research in chemistry, materials science, physics and medicine. [2]. Considering of CNTs, our research is focused on the study of CNT synthesis and growth mechanisms upon thermal chemical vapour deposition, and their electrochemical properties. The functionalization of CNTs, through a chemical attachment of either molecules or functional groups to their sidewalls, is an effective way to improve their solubility and to enhance their physical properties that make them of potentially useful for technological applications ranging from nanoelectronics, sensors and electrochemical devices to composite materials. Graphene is the carbon fourth form: the 2D material graphene, made of carbon atoms arranged in a honeycomb lattice, has its peculiar mechanical, electronic, optical, and transport properties. Many of these features result solely from the symmetry properties of the honeycomb lattice. The chemical modification can be achieved via either covalent or non-covalent interactions. Covalent modifications often destroy some of the graphene conjugation system, resulting in compromising some of its properties.[3]. 1. Gupta, Vinay; Scharff, Peter; et al., C60 intercalated graphite: a new form of carbon. - In: Fullerenes, nanotubes & carbon nanostructures : An international and interdisciplinary journal.: Taylor & Francis, 13.2005, Suppl. 1, S. 427-430, http://dx.doi.org/10.1081/FST-200039421 2. H.M. Kuznietsova, O.V. Lynchak, N.V. Dziubenko, V.L. Osetskyi, O.V. Ogloblya, YuI. Prylutskyy, V.K. Rybalchenko, U. Ritter, P. Scharff, Water-soluble C60 fullerenes reduce manifestations of acute cholangitis in rats // Applied Nanoscience (2018), P.1–8, DOI: 10.1007/s13204-018-0700-5 3. Szroeder,PawełTsierkezos, Nikos G.; Walczyk, Mariusz; Strupi´nski, Włodzimierz; Górska-Pukownik, Agnieszka; Strzelecki, Janusz; Wiwatowski, Kamil; Scharff, Peter; Ritter, Uwe, Insights into electrocatalytic activity of epitaxial graphene on SiC from cyclic voltammetry and ac impedance spectroscopy. - Journal of Solid-state electrochemistry: current research and development in science and technology. - Berlin: Springer, 18 (2014), 9, 2555-2562,

R.3.1
10:30
Authors : Maurizio Prato
Affiliations : Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste, Italy CICbiomaGUNE, San Sebastián, Spain

Resume : Connecting nanostructured materials to biological compartments is a crucial step in prosthetic applications, where the interfacing surfaces should provide minimal undesired perturbation to the target tissue. Ultimately, the (nano)material of choice has to be biocompatible and promote cellular growth and adhesion with minimal cytotoxicity or dis-regulation of, for example, cellular activity and proliferation. In this context, carbon nanomaterials, including nanotubes and graphene, are particularly well suited for the design and construction of functional interfaces. This is mainly due to the extraordinary properties of these novel materials, which combine mechanical strength, thermal and electrical conductivity. Our group has been involved in the organic functionalization of various types of nanocarbons, including carbon nanotubes and graphene. The organic functionalization offers the great advantage of producing soluble and easy-to-handle materials. As a consequence, since biocompatibility is expected to improve upon functionalization, many modified carbon nanomaterials may be useful in the field of nanomedicine. In particular, we have shown that carbon nanotubes can act as active substrates for neuronal growth, a field that has given so far very exciting results. Nanotubes are compatible with neurons, but, especially, they play a very interesting role in interneuronal communication. Improved synaptic communication is just one example. Research toward nerve regeneration in damaged tissues has also given very interesting results. During this talk, we will show the latest and most exciting results obtained in our laboratories in these fast developing fields.

R.3.2
11:00
Authors : Ji-Young Hwang, Seung Beom Kang, Sang Won Lee, Ueon Sang Shin, Sang-Hoon Lee
Affiliations : Research Center for Carbon Convergence Materials, Korea Institute of Carbon Convergence Technology (KCTECH), Jeonju, Republic of Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea; Department of Biomedical Engineering, Korea University, Seoul, Republic of Korea jyyellow@gmail.com

Resume : Engineering of the surface is the multidisciplinary research of materials science and has a broad range of applications to chemistry, biology, engineering and medicine. Surface modification of a biomaterial can be done by different methods for altering into required characteristics, such as size, morphology, topology, wettability, roughness, surface charge, reactivity, biocompatibility and applicability. Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions of tissues. Here we provide an overview of the various applications of our surface modification systems and also discuss successes to date, current limitations and future directions. We developed surface modification system of nanotubes, nanoparticles, polymers, plastic wares, polydimethylsiloxane chips, using chemicals, zwitterionic polymers, polyethylene glycol (PEG), 3-aminopropyltriethoxysilane (APTES), extracellular matrix (ECM), other bioactive molecules, etc for efficiently enhancing cell functions. Our results highlight an innovative surface-modified biomaterials for advance cell culture and tissue regeneration system and may allow the development of enabling technologies for tissue engineering and regenerative medicine applications.

R.3.3
11:20
Authors : Hsing-An Lin1,2,3, Yoshikatsu Sato4, Yasutomo Segawa2,3, Taishi Nishihara2,3, Kakishi Uno3, Lawrence T. Scott5, Tetsuya Higashiyama3,4, Kenichiro Itami2,3,4
Affiliations : 1 School of Materials Science and Engineering, Shanghai University, Shanghai, 200444, China. 2 JST ERATO, Itami Molecular Nanocarbon Project, Chikusa, Nagoya 464-8602, Japan. 3 Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan. 4 Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Japan. 5 Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467-3860, USA

Resume : Incorporating hydrophilic tetraethylene glycol with saddle-shaped nanographene make water-soluble warped nanographene (WNG) has good solubility in organic solvents and water. It exhibits strong greenyellow fluorescence, high photostability, and low cytotoxicity. The aforementioned properties imply that water-soluble WNG is applicable to live-cell imaging. Subsequently, water-soluble WNG was applied for HeLa cell imaging. The results indicated that water-soluble WNG internalized in HeLa cells and accumulated into the lysosome as aggregates. During live-cell imaging by water-soluble WNG, we serendipitously discovered that the HeLa cells stained with water-soluble WNG could be killed by selective laser irradiation. In the presence of 5.0 mM of water-soluble WNG, the light irradiated HeLa cell shrunk, generated blebs, and finally died. Moreover, dose-depended cell viability is observed. The cell viability was 98% in the presence of 0.01 mM of water-soluble WNG. Upon increasing the concentration of water-soluble WNG from 0.01 to 1.0 mM, a dramatic decrease in cell viability was observed, and 91% of the cells were killed in the presence of 1.0 mM water-soluble WNG under irradiation. Using 5.0 mM water-soluble WNG, almost no cells survived under the irradiation. In contrast, even in the presence of water-soluble WNG, no obvious change in cell morphology and viability was observed without light stimulation. Although the mechanism is unclear, the relatively high efficiency of the singlet oxygen generation of water-soluble WNG may contribute to its HeLa cell death. [1] Hsing-An Lin, Yoshikatsu Sato, Yasutomo Segawa, Taishi Nishihara, Kakishi Uno, Lawrence T. Scott, Tetsuya Higashiyama, Kenichiro Itami, Synthesis of a water-soluble warped nanographene and its application for photo-induced cell death. Angew. Chem. Int. Ed. 2018, 57, 2874-2878. (VIP)

R.3.4
11:40
Authors : Jun Maruyama, Tsutomu Shinagawa, Mitsuru Watanabe, Yukiyasu Kashiwagi, Shohei Maruyama, Toru Nagaoka, Wakana Matsuda, Yusuke Tsutsui, Shu Seki, Hiroshi Uyama
Affiliations : Osaka Research Institute of Industrial Science and Technology; Kyoto University; Osaka University

Resume : Cylindrical self-assembly of uniform polystyrene nanoparticles with fructose was carried out by hydrothermal treatment in the presence of both carbon nanofiber and sodium alginate, which is followed by heat treatment in an inert atmosphere. The carbonization generated fructose-derived honeycomb-like carbon walls with helically-aligned nanopores left after the polystyrene decomposition. The diffuse reflectance circular dichroism measurements gave peaks with opposite signs for the D- and L-fructose-derived cylindrical carbons. Circularly polarized light sensitivity in transient photoconductivity was confirmed apparently in the carbon-based helical structures. This sensitivity as well as straightforward formation of composites with another component to give helicity showed potential applications of the helically-aligned pores.

R.3.5
12:00
Authors : Rachel Yerushalmi - Rozen, Evgenee Yekymov, Eugene Katz,
Affiliations : Dept. of Chemical Engineering Ben-Gurion University of the Negev Israel; Dept. of Solar Energy and Env. Phys. the, Jacob Blaustein Institute for Desert Research, BGU

Resume : Exohedral van der Waals (vdW) hybrids comprising carbon nanotubes (CNT) and fullerene molecules (C60) may be used for engineering of carbon nano-materials with tunable physiochemical properties. As the hybrids preserve the sp2 hybridization of the components, superior electron transport, low percolation threshold, good electron accepting and efficient singlet oxygen sensitizing ability are expected. These hybrids may be useful as electron acceptors and charge carriers in bulk-heterojunction organic photovoltaics (OPV). In the reported study we demonstrate that induced SP3 point defects in CNT (via UV/O3 treatment) can be used for controlling the nano-structure of CNT- C60 hybrids. We describe a two-step process for assembly of CNTs networks and thermal (vacuum) sublimation of fullerenes nanocrystals. Transmission electron microscopy indicates that the hybrids comprise of random 3D scaffolds of individual CNT and fullerite nanocrystals, and Raman analysis reveals their non-covalent nature. While surface migration of the fullerenes on the CNT surface leads to coarsening of the nanocrystals, we find that controlled induction of SP3 point defects in the CNT can trap the initial nano-morphology and preserve the nano-dimensions and structure of the fullerene crystals, also at elevated temperatures and prolonged annealing. The nano-morphology of the treated nanotubes is preserved also when the hybrids are coated by a polymer layer (poly(3-hexylthiophene-2,5-diyl, P3HT), and the polymer-hybrid films show significant quenching of the photoluminescence, indicating that these hybrids could be useful in photovoltaic applications.

R.3.6
12:20
Authors : Prabhat Kumar, Martin Šilhavík, Zahid Ali Zafar, and Jiří Červenka
Affiliations : Department of Thin Films and Nanostructures, FZU - Institute of Physics of the Czech Academy of Sciences, Prague 16200, Czech Republic

Resume : We demonstrate an ultrasensitive strain-gauge sensor using a conductive and flexible 3D graphene aerogel that enables detecting small pressures and various biological signals from the movement of human skin. The active element of the sensor is composed of a 3D graphene nanoporous framework made of graphene sheets separated with air-filled pores. The 3D graphene aerogel is synthesized using a simple hydrothermal technique followed by freeze-drying and thermal annealing. The resulting graphene aerogel is elastic and exhibits a significant change of electrical resistance with strain. It is found that the resistance of the sample is highly sensitive to applied strain, enabling the detection of small changes of the sample size down to a few micrometers. The responsivity of the samples allows detecting fast events down to a few microseconds. We show that the 3D graphene-based strain-gauge can be used as a wearable electronic sensor for human heartbeat monitoring as well as for other kinds of biological motion detection.

R.3.7
12:40
Authors : Cathal Larrigy, Eoghan Vaughan, Nadim Shahin, Alessandra Imbrogno, Pingyang Ma, Daniela Iacopino, Micheal Burke, and Aidan J. Quinn
Affiliations : Tyndall National Institute, University College Cork,

Resume : There is increasing interest in laser methods for direct-write formation of graphitic and graphene-like carbon structures from polymers, especially under ambient conditions. Applications include direct incorporation of sensing functionality (e.g., electrochemical, temperature, humidity, strain) onto plastic components. While the most popular material for laser-induced graphitization is polyimide, laser-induced graphitization of renewable materials through “multiple lasing” processes has been explored recently. Chitosan, the second most abundant natural polysaccharide after cellulose, is derived from partial deacetylation of chitin (found in insect exoskeletons, some crustaceans and fungi cell walls). Here we report on site-selective laser-induced formation of porous 3D graphene on flexible and water-soluble chitosan films using low-cost lasers. Raman spectroscopy demonstrates formation of high-quality graphene through the presence of sharp 2D peak. Measured full-width at half maximum intensity values (FWHM) values for the 2D peak were FWHM(2D) < 100 cm-1 (wavenumbers). We have also demonstrated proof-of-principle electrochemical sensing of with two redox systems: outer-sphere [Ru(NH3)6]3+/2+ and inner-sphere [Fe(CN)6]3-/4-. Quasi-reversible Nernstian behavior was observed in cyclic voltammetry measurements for both systems over time scales ~ 20 minutes. Measurements at longer time-scales showed evidence of electrode degradation, consistent with dissolution of the biopolymer substrate. These results showcase the potential of these exciting materials for development of compostable sensors which could be used in sustainable packages for perishable food, e.g., dairy or meat products.

R.3.8
13:00 14:00 Lunch Break    
14:00
Authors : Mandeep Singh, Dr. Ashish Gupta, Dr. S. R Dhakate, Dr. Shashank Sundriyal
Affiliations : 1. CSIR-National Physical Laboratory, New Delhi, India 2. Academy of scientific and innovative research, NPL campus, New Delhi

Resume : PAN (Polyacrylonitrile), Rayon and Pitch are three main precursors for carbon fibers where PAN is used mostly due to its good thermal stability, high carbon content and good tensile strength. However, PAN-based carbon nanofibers limit its use in bulk due to the high cost of precursor i.e. PAN. So there is a need to develop carbon fibers from low-cost precursors. Biopolymers such as lignin and cellulose have high carbon content and have the potential to become a low-cost precursor due to their abundance in nature. Lignin is the second most abundant biopolymer after cellulose. Due to the highly complex structure of lignin, it is very difficult to spun it alone in the form of fibers. So, we have blended lignin with PVA (polyvinyl alcohol) and spun the blend using electrospinning. In this work, we present the optimization and synthesis of carbon nanofibers from Lignin: PVA blend using the electrospinning technique. Further, these mats were characterized by their electrochemical properties. SEM analysis shows that lignin: PVA based carbon nanofibers have a diameter in range 600-850 nm. These nanofiber mats were further characterized for their microstructural, thermal and electrical properties using XRD, RAMAN, TGA, DSC, BET. These mats show a specific capacitance of 145F/g at 1A/g.

R.3.9
14:20
Authors : 1 Antonio Riul Jr., 2 Anerise de Barros, 1 Gabriel Gaal, 1 Mawin J. M. Martinez, 2 Carlos A. Avendano, 3 Monica J. Andrade, 2 Manuel Quevedo-Lopez, 1 Fernando Alvarez, 3 Ray H. Baughman
Affiliations : 1 University of Campinas, Department of Applied Physics, Institute of Physics ?Gleb Wataghin?, Campinas, SP, BR; 2 University of Texas at Dallas, Materials Science Department, Dallas, TX, USA; 3 University of Texas at Dallas, NanoTech Institute, Dallas, TX, USA

Resume : Self-healing materials inspire the next generation of multifunctional wearables and IoT appliances. Nonetheless, it is crucial to fabricate thin films enabling seamless and conformational coverage irrespective of the complexity of shape and geometry of the surface for electronic skins, smart textiles monitoring body signals, soft robotics, and wearable devices storing energy. Within this context, the layer-by-layer (LbL) technique is a versatile approach for homogeneously dispersing materials into various matrices. Moreover, it provides molecular level thickness control and conformational configuration on virtually any surface. Poly(ethyleneimine) (PEI) and poly(acrylic acid) (PAA) are materials primarily employed in LbL structures due to their intrinsic ability to form restorative composite coatings at room temperature. However, it is still challenging to achieve thin films having high conductivity, good healing strength, and controlled mechanical properties at ambient conditions. Here, PEI and PAA were mixed with conductive materials such as gold nanorods, poly(3,4-ethylene dioxythiophene) polystyrene sulfonate (PEDOT: PSS), reduced graphene oxide and multi-walled carbon nanotubes in distinct LbL film architectures. Electrical (AC and DC), optical (Raman spectroscopy), and mechanical (nano-indentation) measurements were performed to evaluate changes occurring in multilayered structures. Self-healing tests in some composites indicated multiple healings at the same damaged area, without changes in the electrical properties. The results are promising for the manufacture of self-healing conductors by design, as the mechanical properties were balanced with the healing and electrical efficiencies. The formed nanostructures have the potential for creating smart surface layers having unique features that solve technical challenges.

R.3.10
14:40
Authors : Lamnini S, a b Károly Z, c Bódis E, c Balázsi K, b Balázsi C, b
Affiliations : a Doctoral School of Material Science and Technologies, Óbuda University, Bécsi str.96/B, 1034 Budapest, Hungary b Institute for Technical Physics and Materials Science, Centre for Energy Research, Hungarian Academy of Sciences, Konkoly – Thege M. str. 29-33, 1121 Budapest, Hungary c Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary

Resume : Composites consisting of 8 mol.% yttria-stabilized zirconia (8YSZ) and 1 wt%, 5 wt% and 10 wt% multiwall carbon nanotubes (MWCNTs) have been prepared by attrition milling and spark plasma sintering (SPS). The effect of sintering temperature and MWCNT content on the microstructural features including apparent density, phase transition, crystal size and mechanical properties were investigated. The phase transformation during the sintering process was observed with X-ray diffraction. The MWCNT stability was investigated by Raman spectroscopy. Vickers hardness and indentation fracture toughness of 8YSZ/MWCNTs composites were evaluated and compared with reference 8YSZ composite. The crack propagation mechanisms of composites were determined. MWCNT pull-out, crack bridging and crack deflections were found and constituted a key factor of fracture toughness enhancement in the composites with MWCNT addition. DOI: 10.1016/j.ceramint.2018.11.207

R.3.11
15:00
Authors : Yu Zhao, Yi Tao, Kabin Lin, Jingjie Sha and Yunfei Chen
Affiliations : School of Mechanical Engineering, Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China

Resume : In this work, in order to elucidate the intrinsic mechanism of thermal transport along different crystallographic planes in layered materials, we propose a simple and convenient method to manufacture inclined graphite applying Focused Ion beam (FIB). Then, the thermal conductivities along several crystallographic planes in graphite at room temperature are measured using the time-domain thermoreflectance (TDTR) method. To address the issue of lacking in-plane symmetry in inclined graphite, a three dimensional thermal model is developed to extract the thermal conductivity for layered materials from the measured signals of the TDTR method. In addition, for comparison with the cut graphite samples and extracting the in-plane and cross-plane thermal conductivities of graphite sample, a piece of graphite flake is prepared to conduct the TDTR experiment. Our experimental results show that the processing method is reliable and validate the three dimensional thermal model which we develop is appropriate to describe the thermal transport in layered materials. More important is that the results also provide a direct experimental evidence for the classical anisotropy model derived from the Fourier’s law can well predicting the thermal transport along an arbitrary crystallographic plane in layered materials. Furthermore, for graphite, after phonons undergoing the sufficient scattering processes, the in-plane phonon modes dominate the process of thermal transport along all crystallographic planes except the cross-plane direction owing to the value of the in-plane thermal conductivity is much larger than that of the cross-plane conductivity. Our study provides a new perspective on the thermal transport in graphite and other layered materials, and provides an important design guideline for actively manipulating the direction of thermal transport in nano-layered materials.

R.3.12
15:40 16:00 Coffee Break    
 
ROUND TABLE DISCUSSION ON FRONTIER RESEARCH Invited 7en minutes Key Speech on Frontier Research : Organizer/Chair Pofessor Hsing-An Liu (Shanghai University,China; Nagoya Univesity, Japan)
17:00
Authors : Oleksandr Ivanyuta1, Eugenia Buzaneva2, Uwe Ritter3, Peter Scharff3
Affiliations : 1 Faculty of Radiophysics Electronics and Computer Systems, Taras Shevchenko National University of Kyiv, Ukraine E-mail: iva@net.univ.ua 2 The Scientific and Training Center on Physical and Chemical Material Science, National Taras Shevchenko National University of Kyiv and NAS of Ukraine 3 Institute of Chemistry & Biotechnology, T U Ilmenau, Germany

Resume : Biofunctionalization of carbon nanotubes, which are well-known for their unique complex of properties, is in the centre of research attention for numerous biomedical applications, also as for fundamental study of nanotubes as templates. Covalent or non-covalent immobilization of biomolecules on carbon nanotubes opens the doors for novel biocomposite manufacturing and biosensing. Carbon nanotube functionalization by DNA, RNA, oligonucleotides or single nucleotides is of special interest as possible approach for nucleic acid sensing, gene delivery, gene therapy, clinical diagnosis and pathogen monitoring. It is also used in nanotechology as technique for suspensioning, characterization and separation of nanotubes. In our investigations the non-covalent immobilization in aqueous suspensions of AMP and DNA on multi-walled carbon nanotubes developed. Previous oxidation of multi-walled carbon nanotubes by ozonolysis were used if it was necessary. The characterization of functionalized nanotubes was carried out by Scanning Electron Microscopy, Transmission Electron Microscopy, Optical Microscopy, Raman, UV-visible-NIR, IR- and Photoluminescence Spectroscopies. Various models of interface formation between biomolecules and nanotube surface are discussed. Optical characteristics of novel biofunctionalized nanotubes also gave us necessary information for their future application as biosensors or activating agents.

R.RT2.1
17:00
Authors : Jun Maruyama, Tsutomu Shinagawa, Mitsuru Watanabe, Yukiyasu Kashiwagi, Shohei Maruyama, Toru Nagaoka, Wakana Matsuda, Yusuke Tsutsui, Shu Seki, Hiroshi Uyama
Affiliations : Osaka Research Institute of Industrial Science and Technology; Kyoto University; Osaka University

Resume : Potential applications of cylindrical carbon with helically aligned pores were explored based on its concomitant properties. The first example is transient photoconductivity (ϕΣμ), in which ϕ is the photocarrier generation yield and Σμ is the sum of the charge-carrier mobilities. The flash-photolysis time-resolved microwave conductivity (FP-TRMC) technique was conducted using circularly polarized light excitation at 532 nm. The ϕΣμ difference at the cylindrical carbon synthesized using D-fructose was observed for the left- and right-handed circularly polarized light. In contrast, no appreciable ϕΣμ difference was observed for the cylindrical carbon synthesized using the racemic fructose. These results indicated the potential to form an optoelectronic device that could change the conductivity by the circularly-polarized light. The second example is the feasibility to form composites with the helical structure due to easy access of the constituents to the pores. A composite of the cylindrical carbon and colloidal Au nanoparticles (Au NPs) was formed by immersing the cylindrical carbon in the colloidal Au dispersion in toluene and rinsing with the dispersion medium. The Au NPs clusters were helically aligned like stepping stone. This simple formation method enabling the helical alignment of the Au NP clusters implied the potential of the cylindrical carbon as a platform for feasible generation of helical structures.

R.RT2.2
17:00
Authors : Hsing-An Lin1,2,3, Yoshikatsu Sato4, Yasutomo Segawa2,3, Taishi Nishihara2, Kakishi Uno3, Lawrence T. Scott5, Tetsuya Higashiyama3,4, Kenichiro Itami2,3,4
Affiliations : 1. Department of Polymer Materials, Shanghai University, Baoshan, Shanghai, 200444, China.; 2. JST ERATO, Itami Molecular Nanocarbon Project, Chikusa, Nagoya 464-8602, Japan.; 3. Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan.; 4. Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Japan.; 5. Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467-3860, USA

Resume : Nanographene, a small piece of graphene, has attracted unprecedented interest across diverse scientific disciplines particularly in organic electronics.1 The biological applications of nanographenes such as bioimaging, cancer therapies, and drug delivery would provide significant opportunity and breakthrough in the field. However, intrinsic aggregation behavior and low solubility of nanographenes stemming from the flat structures hamper their development in bioapplications. Herein, we report a water-soluble warped nanographene (WNG)2 that can be easily synthesized by a sequence of regioselective C–H borylation and cross-coupling of saddle-shaped WNG core structure. The high water solubility of water-soluble WNG is ensured by its saddle-shaped structure with hydrophilic tetraethylene glycol chains. The water-soluble WNG possesses a range of additional promising properties such as long lifetime fluorescence, good photostability and low cytotoxicity. Moreover, the water-soluble WNG has been successfully applied for live HeLa cell imaging and photo-induced cell death.

R.RT2.3
17:00
Authors : 1 Antonio Riul Jr., 2 Anerise de Barros, 1 Gabriel Gaal, 1 Mawin J. M. Martinez, 2 Carlos A. Avendano, 3 Monica J. Andrade, 2 Manuel Quevedo-Lopez, 1 Fernando Alvarez, 3 Ray H. Baughman
Affiliations : 1 University of Campinas, Department of Applied Physics, Institute of Physics ?Gleb Wataghin?, Campinas, SP, BR; 2 University of Texas at Dallas, Materials Science Department, Dallas, TX, USA; 3 University of Texas at Dallas, NanoTech Institute, Dallas, TX, USA

Resume : Self-healing materials inspire the next generation of multifunctional wearables and IoT appliances. Nonetheless, it is crucial to fabricate thin films enabling seamless and conformational coverage irrespective of the complexity of shape and geometry of the surface for electronic skins, smart textiles monitoring body signals, soft robotics, and wearable devices storing energy. Within this context, the layer-by-layer (LbL) technique is a versatile approach for homogeneously dispersing materials into various matrices. Moreover, it provides molecular level thickness control and conformational configuration on virtually any surface. Poly(ethyleneimine) (PEI) and poly(acrylic acid) (PAA) are materials primarily employed in LbL structures due to their intrinsic ability to form restorative composite coatings at room temperature. However, it is still challenging to achieve thin films having high conductivity, good healing strength, and controlled mechanical properties at ambient conditions. Here, PEI and PAA were mixed with conductive materials such as gold nanorods, poly(3,4-ethylene dioxythiophene) polystyrene sulfonate (PEDOT: PSS), reduced graphene oxide and multi-walled carbon nanotubes in distinct LbL film architectures. Electrical (AC and DC), optical (Raman spectroscopy), and mechanical (nano-indentation) measurements were performed to evaluate changes occurring in multilayered structures. Self-healing tests in some composites indicated multiple healings at the same damaged area, without changes in the electrical properties. The results are promising for the manufacture of self-healing conductors by design, as the mechanical properties were balanced with the healing and electrical efficiencies. The formed nanostructures have the potential for creating smart surface layers having unique features that solve technical challenges.

R.RT2.4
18:30 AWARD CEREMONY Graduate Student followed by SOCIAL EVENT    
19:55 SOCIAL EVENT    
Start atSubject View AllNum.
 
Stimuli - Responsive Materials, Surfaces/Interfaces and Systems Multi-Functionality SESSION Invited Presenters : Organizer/Chair Dr. Donata Iandolo (Ecole des Mines de Saint-Etienne, University of Lyon, Universite Jean Monnet, INSERM, France)
08:50
Authors : Donata Iandolo, a,b Jonathan Sheard,c Galit Katarivas Levy,d Charalampos Pitsalidis,b Athina E. Markaki,d Darius Widera,c Rόisín M. Owensb
Affiliations : a Mines Saint-Etienne, University of Lyon, Université Jean Monnet, INSERM, France donata.iandolo@emse.fr b Department of Chemical Engineering and Biotechnology, University of Cambridge, United Kingdom c School of Pharmacy, University of Reading, United Kingdom d Department of Engineering, University of Cambridge, United Kingdom

Resume : Osteoporosis is a skeletal disease characterized by bone loss and bone microarchitectural deterioration. The increasing life expectancy calls for innovative and effective approaches to compensate for bone loss. Due to their well-documented regenerative and anti-inflammatory potential, stem cells represent a promising option. The knowledge of bone piezoelectricity and of bioelectricity as a further cue to influence cell fate, in addition to biochemical and mechanical ones, has elicited for the use of physical stimulation together with electroactive materials as smart alternatives for bone tissue engineering. The combination of smart substrates, stem cells and physical stimulation to induce cell differentiation is therefore a new avenue in the field. Biomimetic scaffolds were prepared by combining the conducting polymer PEDOT:PSS with collagen type I, the most abundant protein in bone. Pores sizes, mechanical and impedance properties were measured as a function of scaffolds composition. Two populations of stem cells were used to understand the impact of scaffold composition on cell behaviour. Preliminary electrical stimulation experiments were run to evaluate its use in combination with the developed electroactive scaffolds in the frame of a bioelectronic approach to bone tissue engineering. Recent results on devices developed to assess stem cell differentiation will be presented.

R.4.1
09:10
Authors : Darius Widera
Affiliations : University of Reading, School of Pharmacy, Stem Cell Biology and Regenerative Medicine Group Hopkins Building, Room 108 Reading RG6 6UB United Kingdom

Resume : Since its early days, ex vivo mammalian cell culture has been conducted on flat two-dimensional (2D) glass or polystyrene surfaces. Although 2D cell culture is still widely used, it is known to result in unnatural cell polarity and morphology in addition to lacking the three-dimensional extracellular matrix. These drawbacks are especially evident if the cultivated cells are clinically relevant cell types including but not limited to stem cells. In this context, 2D cultivation is known to interfere with stem cell proliferation and to alter the cell phenotype. In this talk, different approaches to stem cell cultivation, differentiation, and assessment of migration in 3D will be discussed. In particular, biocompatible natural scaffolds for the cultivation of adult human neural crest-derived stem cells and human mesenchymal stem cells of different origins will be presented. Moreover, the impact of 3D cell culture on the regenerative potential of stem cell secretome will be discussed.

R.4.2
09:30
Authors : Gianni Ciofani, Attilio Marino, Christos Tapeinos
Affiliations : Istituto Italiano di Tecnologia, Smart Bio-Interfaces, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy

Resume : Central nervous system tumors are an important cause of morbidity and mortality worldwide. Among them, glioblastoma multiforme is the most aggressive and lethal, characterized by extensive infiltration into the brain parenchyma [1]. In our research we propose nanotechnology-based approaches able to improve both the drug anti-cancer efficacy and its delivery efficiency across the blood-brain barrier. To achieve these aims, we exploit hybrid nanovectors composed by lipids and superparamagnetic iron oxide nanoparticles [2]. The fabricated nanovectors demonstrate an enhanced release after exposure in an alternating magnetic field, and a complete release of the encapsulated drug (temozolomide chosen as model) after the synergic effect of low pH, increased concentration of hydrogen peroxide, and increased temperature due to the applied hyperthermia. The optimal anticancer effects result from the synergic combination of hyperthermal chronic stimulation with the controlled temozolomide release, highlighting therefore the potential of the proposed drug-loaded lipid magnetic nanovectors for the treatment of glioblastoma multiforme. An in-depth proteomic analysis corroborated the findings obtained on a complex multi-cellular in vitro model [3]. Acknowledgments This project has received funding from the European Research Council (ERC) under the European Union?s Horizon 2020 research and innovation program (grant agreement N°709613, SLaMM). References [1] Kim S.S., [?], Chang E.H. Biochem. Biophys. Res. Commun. 468: 485-489 (2015) [2] Tapeinos C., [?], Ciofani G. Nanoscale 11: 72-88 (2019) [3] Marino A., [?], Ciofani G. Nanoscale 11: 21227-21248 (2019)

R.4.3
09:50
Authors : Gabriela Romero, Rohini, Guntnur, Tina Rodgers
Affiliations : Department of Biomedical Engineering and Chemical Engineering, The University of Texas at San Antonio

Resume : Despite the growing evidence supporting the use of biocompatible materials as vehicles to deliver therapeutics, their clinical application is often limited by challenging biological environments such as accessing the brain due to the blood-brain barrier (BBB). The BBB hinders most molecules from entering the central nervous system from the blood stream, limiting the delivery of drugs and materials into the brain for the treatment of brain malfunctions. Soft matter engineering offers a powerful approach to the design and synthesis of effective systems to manipulate cellular signals and behaviors. Here, we investigate soft biomedical materials as minimally-invasive and transgene-free alternatives for the treatment of brain disorders, including safer strategies to bypass the blood brain barrier, and the development of new technologies for wireless neuromodulation and gene-editing therapies. Nanoscale heating effects of magnetic nanoparticles under alternating magnetic fields has been used to control the thermodynamic transition of thermo-sensitive polymer brushes to release neuromodulatory compounds. This magneto-thermal system allow us to modulate on-demand neural activity, which is critical for the treatment of neurological disorders such as Parkinson disease. In the other hand, pH sensitive biocompatible block co-polymers are being used to fabricate artificial viruses. Gene-editing systems have become an important tool in biological engineering and genome editing, providing programmable platforms for precision gene targeting. These tools have immense promise as therapeutics that could potentially correct disease-causing mutations. Most of the gene-editing approaches use viral vectors to deliver the therapeutic tools, limiting clinical applications due to safety concerns. We investigate stimuli-responsive soft biomedical platforms that enable a synthetic, non-invasive strategy for the delivery of gene-editing tools. Our non-viral delivery approach focuses on mimicking biological organisms? functionalities and engineering them into a synthetic polymeric carrier. Specifically, we have been developing polymeric technologies for the delivery of gene-editing tools across the BBB for the treatment of glioblastoma and other brain cancers.

R.4.4
10:10
Authors : Cheng-Yun Wu, Yu-Hsuan Hsu, Dehui Wan, Yunching Chen, Chieh-Cheng Huang
Affiliations : Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan (Cheng-Yun Wu, Yu-Hsuan Hsu, Dehui Wan, Yunching Chen, Chieh-Cheng Huang)

Resume : Tumor hypoxia not only reduced the efficacy of chemotherapy and radiation therapy but also changed the feature of tumor action. Thus, tumor hypoxia microenvironment needs to be improved urgently. Herein, we report a multifunctional silk fibroin hydrogel (SFG) system that combines artificial and natural enzymes, which integrates platinum-coated-hollow-gold-nanoparticles (HGN@Pt, artificial enzyme) and glucose oxidases (GOx, natural enzyme) in SFG, to develop a starvation and photothermal cancer synergistic therapy. The GOx could consume the intratumoral glucose/oxygen leading to tumor starvation and necrosis, but the strategy was strongly limited in tumor hypoxia microenvironment. Therefore, we designed an efficient, stable biomimetic nanoenzyme (HGN@Pt), which could catalyze the decomposition of H2O2 to generate O2 (e.g., from 0 to 8 mg/L at 37 ? in 10 min). In addition, the HGN@Pt could be utilized as photothermal therapy (PTT) agent due to the strong surface plasmon resonance, thereby converting the near infrared (NIR) light into heat (e.g., from 25 to 50 ? under 1 W/cm2 of 808 nm laser irradiation in 10 min). More importantly, we found that the HGN@Pt display great thermal and catalytic stability, which were advantageous for repeated-photothermal therapy and long-term hypoxia improvement ability, respectively. Moreover, the in vitro experimental results show that the HGN@Pt could catalyze endogenous H2O2 to generate O2, thus the GOx were able to consume glucose to induce starvation therapy, even in hypoxia condition. Note that the H2O2 would be also generated after the oxidation of glucose, which would be converted to O2 by HGN@Pt, again. Therefore, the both catalysts successfully form a complementary circulatory system to continuously consume glucose and generate O2 at tumor site. Finally, we constructed a 4T1 tumor model to investigate the therapeutic efficacy in vivo. The mixture of silk fibroin solution was orthotopically injected at the tumor site, followed by the irradiation of NIR laser for inducing gelation. The multifunctional SFG could slowly release the GOx to tumor microenvironment for long-tern starvation therapy, and trap the HGN@Pt in tumor region to conduct the repeated PTT treatments and continuously improve the hypoxia condition in the treatment period. The results of reduction of tumor tissue hypoxia-inducible factors and tumor inhibition ability clearly demonstrated that the system would efficiently improve tumor hypoxia and successfully inhibit the tumor growth.

R.4.5
10:30 11:00 Coffee Break, General Photo    
11:00
Authors : Katalin Balázsi, Csaba Balázsi
Affiliations : Centre for Energy Research, Konkoly ? Thege M. str. 29-33, 1121 Budapest, Hungary

Resume : The 400 000 artificial hip joint operations made every year in the word and there are 25 000 000 people with a total hip replacement. The wear and risk of the implant loosening increases so that after 10 years 10-20% of the implants have to be renewed. Biomaterials used for implant should possess some important properties in order to long-term usage in the body without rejection. The biocompatibility, mechanical, chemical and surface properties play a key role in the creation of sufficient and long term functional replacements. New fundamental research outcomes with industrial perspectives are given for understanding the applications of ceramics in load-bearing and low-load-bearing bioimplants with directions for future developments. Nowadays, Si3N4 is a new bioceramic with extremely good mechanical properties. Hydroxyapatite (HA) is a widely used bioceramic in implantology considering its high bioactivity. A bioactive coating (HA) on the bioinert ceramic implant?s surface (Si3N4) could help avoid the rejection from the body in the critical early few days after the operation. The preparation of bioceramics will be showed from traditional technologies to novel applications. The main trends and fundamental scientific problems will be discussed.

R.4.6
11:20
Authors : Albena Daskalova
Affiliations : Laboratory of Micro and Nano- Photonics, Institute of Electronics-Bulgarian Academy of Sciences 72 Tzarigradsko chaussee 1784 Sofia Bulgaria E-mail: albdaskalova@gmail.com

Resume : Ultrashort pulse laser interaction has been extensively studied for modification of material properties in various materials. Femtosecond laser applications to microprocessing have received great attention in recent years. This is due to the characteristics of ultra-short laser-material interaction expressed in extremely high-peak power and femtosecond pulse duration which is less than material thermal relaxation time. Moreover, the effects of temperature distribution during material processing can be strongly minimized with ultrashort pulses leading to non-thermal and spatially localized effects that can facilitate volume ablation without collateral thermal damage. Using femtosecond processing laser-induced surface structures provide response by formation of micro/nanoscalestrucutres, which are aquired in respect to laser processing parameters. Such surface feedback can be applied to finely tune and control diverse properties like wettability, reflectivity and biomimetics. Bone disorders have been elucidated as one of the most crucial diseases which can disturb the human health. By simply controlling the laser parameters, diverse surface roughness can be achieved, thus influencing cellular dynamics like adhesion, migration and differentiation which can be tuned via altering topographical properties and chemical composition of the surface. In the current study Titanium and diverse bio and synthetic polymer surfaces were modified by Ti:sapphire femtosecond laser at different laser energies and number of applied laser pulses. Thus, implant surface modifications have been examined, and different laser based surface treatments were applied to obtain structures with hierarchical geometries. The results demonstrate that, the control of the laser parameters can obtain bioactive surfaces by laser modification process.

R.4.7
11:40
Authors : Patrick van Rijn
Affiliations : University of Groningen/ University Medical Center Groningen Department of BioMedical Engineering-FB40

Resume : Nanogels are hydrogel-based nanoparticles that are highly tunable in chemical composition and physicochemical properties. These nanoparticles are highly versatile in their uses and allow for several functions to be combined including antimicrobial properties, fluorescence and MRI tracking and imaging, anti-adhesive, controlled release, and responsiveness to various stimuli. Because of this variety of functions and properties, the particles are used in controlled delivery, imaging, theranostic approaches and functional biomedical multi-modal coatings. The studies presented provide insights in the capabilities of such nanogels, the preparation, modification, and the use of them together with biological systems ranging from in vitro studies to in vivo uses. The ease of scaling, the diversity, and ease of applicability make these particles very powerful and tremendously complement the existing nanotoolbox of liposomes, polymersomes, protein-based nanostructures, and inorganic nanoparticles.

R.4.8
12:00 13:00 Lunch Break    
13:00
Authors : Odair Bim Júnior12 , Ana Bedran-Russo2, Valdecir Farias Ximenes3, Regina C. G. Frem4, Paulo Noronha Lisboa-Filho1
Affiliations : 1 São Paulo State University (Unesp), School of Sciences, Department of Physics, Bauru, Brazil 2 The University of Illinois at Chicago (UIC), College of Dentistry, Department of Restorative Dentistry, Chicago, USA 3 São Paulo State University (Unesp), School of Sciences, Department of Chemistry, Bauru, Brazil 4 São Paulo State University (Unesp), Institute of Chemistry, Department of Inorganic Chemistry, Araraquara Brazil

Resume : Biomimetic syntheses combine intrinsic functions of bio(macro)molecules with the unique characteristics of synthetic materials to yield composites that mimic the complex structure of natural tissues. The biomimetic synthesis of coordination networks such as metal-organic frameworks (MOFs) is a relatively new concept in the family of bioinspired materials. For instance, the integration of MOFs with enzymes and proteins via biomimetic strategies has shown potential ground-breaking applications in biomedicine, controlled delivery, biostorage, and biocatalysis. Our group has pioneered the use of biomimetically-mineralized MOFs in the control of extracellular matrix component degradation, particularly of fibrillar collagen (the most abundant protein of the human body). Microporous MOFs have been synthesized in situ either as crystalline shells to immobilize collagen degrading enzymes or as protective coatings for native collagen fibrils. Restraining enzyme catalysis and protecting the fibrillar collagen surface are strategies that ultimately envisage the control of collagen catabolism as this physiological process when unbalanced, is implicated in numeral pathological conditions (e.g., metastasis, neurodegenerative disorders, osteoporosis, periodontal disease, etc.). Currently, we are using these concepts in biomimetics and supramolecular chemistry to address a core problem in restorative dentistry: the degradation of dental hard tissues by endogenous endopeptidases.

R.4.9
13:20
Authors : Dr.Lavalle Ph., Tallet L., Gribova V., Calligaro C., Vrana N. E.
Affiliations : Lavalle Ph., Tallet L., Gribova V., Calligaro C.: Inserm Biomaterials and Bioengineering, Unit 1121, Strasbourg, France; Lavalle Ph., Vrana N. E., SPARTHA Medical, Strasbourg, France;

Resume : All implantable biomedical systems face several risks once in contact with the host tissue: excessive immune response to the implant and development of bacterial biofilms. A multifunctional surface coating that can address all these two issues concomitantly would significantly improve clinical outcomes. Polyarginine (PAR), a synthetic highly cationic polypeptide, can act on macrophages to control innate immune response and also as an antimicrobial agent due to its positive charges. We developed a new polyelectrolyte multilayer film based on PAR and hyaluronic acid (HA). The layer-by-layer PAR/HA films have a strong inhibitory effect on the production of inflammatory cytokines released by human primary macrophages subpopulations [1]. Next, we show that PAR/HA films were very effective to inhibit pathogenic bacteria associated with infections of medical devices [2] [3]. We demonstrate that exclusively films constructed with poly(arginine) composed of 30 residues (PAR30) acquire a strong antimicrobial activity. This system can also be fabricated in the form of hydrogel [4], useful to provide antibacterial properties to porous implants like surgical meshes. Recent developments to render these systems smart and responsive have also been made to obtain a release and activity only when bacteria are closed to the implants. [1] Özçelik, H. et al. Adv. Healthc. Mater. 2015; 4, 2026-36. [2] Mutschler, A. et al. Chem. Mater. 2016; 28, 8700-09. [3] Mutschler A. et al. Chem. Matter., 2017; 29, 3195?01. [4] Knopf-Marques H. et al. Mat. Sci. Engineer., 2019, C, 104, 109898-07.

R.4.10
13:40
Authors : Eya Aloui(1), Marcella De Giorgi(2), Benoit Frisch(2), Pierre Schaaf(1), Philippe Lavalle(1)
Affiliations : (1) INSERM UMR 1121 Biomaterials and Bioengineering, Institut National de la Santé et de la Recherche Médicale, Université de Strasbourg, Faculté Dentaire de Strasbourg, 11, Rue Humann 67085, Strasbourg Cedex, France; (2) Université de Strasbourg, CNRS, Laboratoire de Conception et Application de Molécules Bioactives UMR 7199, Faculté de Pharmacie, 74 route du Rhin 67401, Illkirch Cedex, France

Resume : Albumin membranes formulated by evaporation in the presence of salt represent a new class of materials (EU patent submitted). In the present study, solutions of bovine serum albumin (BSA) and various salts are evaporated at 37 °C and pH 6 in order to prepare albumin-based material. Among others, NaBr allows the formation of stable and water-insoluble albumin membranes, thus providing a solid material exclusively composed of albumin after thorough washing. The conditions for obtaining BSA/NaBr membranes are assessed, and the molar ratio salt/albumin proves to be a key parameter for their formation. The Young?s modulus (E) of the materials lies around 0.86 ± 0.13 MPa. Biological assays show that these albumin membranes are not cytotoxic, do not induce an inflammatory response and allow cell adhesion. Therefore, due to their interesting properties, these materials are suitable candidates for the development of scaffolds for tissue engineering and biodegradable implantable devices.

R.4.11
13:50
Authors : Mark Schvartzman
Affiliations : Department of Materials Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel

Resume : Immune cells recognize tumors and viral cells by receptors that bind ligands on the membrane of target cells. The receptor signaling is extensively studied today, yet its mechanism is still obscure. Here, we engineered a nanoscale biochip with patterned activating ligands, and used it as an artificial target cell for study of how the ligand arrangement regulate the cytotoxic activity of Natural Killer (NK) cells ? lymphocytes of the innate immune system. To pattern the ligands, we tethered them to sub-10 nm nanoimprinted metallic dots. By tracking the NK cell response to the matrix geometry, we discovered the minimal ligand distribution needed for NK cell stimulation. We also engineered a multifunctional biochip that spatially control activating and inhibitory receptors, whose balance regulate the self-tolerance of the immune system. The chip contained patterned nanodots of two different metals selectively functionalized with activating and inhibitory ligands. We found that the inhibition of NK cell activity is optimized upon ~40 nm segregation between the two ligands, due to constrains of the flexibility of the cell membrane. Finally, we discovered nanoscale mechanosensing of NK cells by interfacing them with ligand-functionalized nanowires. We assessed forces on 10 pN scale applied by the cells onto the nanowires, and found that while neither the nanowires or ligands alone stimulated NK cells, their combination boosted their immune response, in an analogy to ?AND gate? with independent mechanical and chemical logic inputs. Our findings provide an important insight into the mechanism of NK cell function, and introduce novel tools for the study of the cell function with unprecedented spatial and mechanical resolutions.

R.4.12
14:10
Authors : Roman Major1, Roman Ostrowski2, Piotr Wilczek3, 4, Grzegorz Lis5, Antoni Rycyk2, Marek Strzelec2
Affiliations : 1 Institute of Metallurgy and Materials Science Polish Academy of Sciences, Reymonta St. 25 30-059 Kraków, Poland 2 Military University of Technology, gen. Sylwestra Kaliskiego 2, 00-908 Warsaw, Poland 3 The President Stanis?aw Wojciechowski State University of Applied Sciences, Medical Faculty, Kaszubska 13, 62-800 Kalisz 4 Foundation for Cardiac Surgery Development, Wolno?ci St. 345a, 41-800 Zabrze, Poland 5 Jagiellonian University Medical College, Department of Histology, Kopernika St. 7, 31-034 Kraków, Poland

Resume : The aim of the work was to create appropriate substrate for organ transplantation utilizing bioactive tissue-based scaffold populated by cells of graft recipient. The described project consists of few stages aiming to create a fully functional tissue. Aortic valves of animal origin were utilized in the study and they were provided background to produce biocompatible tissue for transplantation into humans. Foreign (host) cells are highly immunogenic components of the graft and they are most responsible for graft rejection. Firstly, host cells were removed (decellularization) by various physical methods (acoustic beam, laser light irradiation) without degradation of the extracellular matrix. These methods differ in their effectiveness and they may also cause destructive effect on components of the extracellular matrix (eg. collagen and elastic fibers) resulting in change of biomechanical properties of the graft. Thus decellularization process was controlled by histological and molecular methods. Samples of decellularized valves and aortic wall were fixed for 48 hours in 4% buffered paraformaldehyde and then they were subjected to a routine paraffin embedding. Briefly, each valve was dehydrated in ethanol (in increasing concentrations: 50%, 70%, 80%, 96% alc., absolute alcohol 1, absolute alcohol 1). Then the material was divided into the following parts: (i). ring of the aorta above leaflet, (ii). left side of the leaflet (subjected to decellurization process), (iii). right side of the leaflet (control). Then, the samples were cleared (two changes of xylene), embedded in paraffin (56ºC, two changes of paraffin) finally paraffin blocks was formed. 5 ?m-thick sections were cut on microtome (i). in a plane transverse to the long axis of the aorta (ring of the aorta) and (ii). in plane parallel to the long axis of the aorta (leaflets, left and right fragment). The sections were mounted on poly-L-lysine coated slides, then dewaxed, rehydrated and subjected to histological and histochemical stainings. One of the new tissue decellularization techniques was laser method, carried out using basic radiation of the Nd:YAG laser at wavelength of 1064 nm or its second harmonic at 532 nm. In order to obtain the most pure extracellular matrix with a minimum number of defects. Different beam patterns, different fluencies and numbers of pulses constituting a single exposure were applied. All exposures were carried out with laser pulses of 10-15 ns in duration at a repetition rate of 5 Hz. In the case of a Gaussian beam, irradiations were carried out at an average fluencies of 169 mJ/cm2 and 83 mJ/cm2 for 1064 nm and 109 mJ/cm2 and 56 mJ/cm2 for 532 nm. The number of pulses in a single exposure cycle was 10, 50, 100, 200 or 500. By inserting a scattering window and focusing lens into the beam path, the samples could be irradiated with radiation with a homogeneous distribution of energy in a circular field with a diameter of 5 mm. The irradiations were carried out at 1064 nm, with a fluency of 76 mJ/cm2 or 56 mJ/cm2. A single exposure consisted of 600, 1000, 2000 or 3000 pulses. Direct laser interference lithography was also used for tissue decellularization. The distribution of laser radiation was in the form of circular spots with a diameter of 5.2 µm spaced apart by about 9.1 µm. Irradiations were carried out at 532 nm. The energy of the pulse was 40 mJ, 20 mJ or 10 mJ. 500, 1000 or 2000 pulses were used in a single exposure cycle. Processes were supported by spectroscopic measurements of aortic tissue samples, which allowed to estimate the absorption coefficients, equal to 4.4 cm-1 for 1064 nm and 3.7 cm-1 for 532 nm. The work was performed as part of project OPUS 12, UMO-2016/23/B/ST8/01481 funded by the National Science Centre (NCN) of Poland.

R.4.13
14:20
Authors : Lenka Zajickova, Lucie Blahova, Ivana Nemcakova, Lucie Bacakova, Vojtech Kupka, Lucy Vojtova, Eva Dvorakova, Anton Manakhov, Miroslav Michlicek, Petr Rysanek
Affiliations : CEITEC - Central European Institute of Technology, Masaryk University (MU) Brno, Czechia; CEITEC MU, Brno, Czechia; Institute of Physiology of the Czech Academy of Sciences, Prague; Institute of Physiology of the Czech Academy of Sciences, Prague; CEITEC Brno University of Technology & Palacký University in Olomouc, Czechia; CEITEC Brno University of Technology, Czechia; CEITEC & Faculty of Science MU, Brno, Czechia; Scientific Institute of Clinical and Experimental Lymphology, ICG SB RAS, Novosibirsk, Russian Federation; CEITEC & Faculty of Sciences MU, Brno, Czechia; Faculty of Science, Jan Evangelista Purkyn? University, Ústí nad Labem, Czechia

Resume : Regenerative medicine stands before the problem to replace non-functional tissues. The success of transplantations complicates by immune reactions or deficiency of donor tissues. Therefore, many laboratories try to develop resorbable tissue scaffolds that could support the patient´s cells. The scaffold material should be biocompatible, biodegradable, and easy to manufacture, thus economically viable. A possible answer is to produce a structure made of a biodegradable polymer that mimics extracellular matrix (ECM), which would be peacefully received and gradually degraded when the new tissue has formed. One of the promising polymers is FDA-approved polycaprolactone (PCL) due to its relatively low cost, excellent processability and mechanical properties, non-toxicity and low immunogenicity. However, the pristine form of PCL has a bioinert and hydrophobic surface causing problems with protein adsorption resulting in reduced cell adhesion. Our previous publications showed that PCL nanofibrous mats could be efficiently modified by plasma polymerization, which leads to the formation of bioactive surface exhibiting increased cell attachment and proliferation, and offering a possibility to attach proteins. In this work, we want to discuss electrospinning of PCL and poly(ethylene oxide) (PEO) mixtures and the modification of electrospun mats by amine plasma polymerization. Based on the structural analysis, we disclose the filamentary sub-structure of PCL/PEO nanofibers and show how their functional properties are varying with varied PCL:PEO ratio and plasma processing. The cell experiments reveal that amine plasma polymer modifications improve the initial adhesion of vascular smooth muscle cells (VSMC), and the optimized plasma polymers increase the proliferation and metabolic activity of VSMC. The evaluation of potential immunogenicity of amine-rich films does not show any significant increase in TNF-alpha or IL-1 beta secretion by RAW 264.7 macrophages. Thus, biodegradable PCL or PCL/PEO nanofibers coated by amine plasma polymers are a promising material for vascular grafts manufacturing.

R.4.14
14:30
Authors : Stefania Vitale, Caio H. N. Barros, Henry Devlin, Stephanie Fulaz, Dishon W. Hiebner, Laura Quinn, Eoin Casey
Affiliations : UCD School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Ireland

Resume : Nanoparticles (NPs) have emerged as promising tools for the control of unwanted biofilms. However, a fundamental understanding of the role of the biofilm extracellular polymeric matrix (EPS) in NP-biofilm interactions is currently lacking. This work analyses how the EPS matrix affects the interaction between engineered NP and biofilms, thus influencing NP diffusion and bioaccumulation. Two strains of Pseudomonas biofilms were exposed to various families of fluorescent engineered silica NPs, synthesised with different surface composition and charge (functionalisation with epoxy-, amino-, PEG- or alkyl-groups). When NPs were exposed to EPS extract solutions, significant differences in the biomolecular corona thickness and composition were observed for different NP surface functionalisation approaches. The same variation was observed in biofilm experiments: particles with the same charge and size but different surface show a very different uptake (up to 40%) and distribution patterns across the biofilm thickness. Differences were also observed for the same particles interacting with different biofilms. The data show that NP surface chemistry dictates the interaction with the biofilm and selective interactions with the EPS take place according to specific surface functionalisation. The results support the rational design of functional NPs as a platform for the development of NP-based anti-biofilm technology that will overcome the challenges associated with the EPS matrix.

R.4.15
14:45
Authors : Christian Goldhahn, Mark Schubert, Ingo Burgert, Munish Chanana
Affiliations : Christian Goldhahn, Mark Schubert, and Ingo Burgert: ETH Zürich, Institute for Building Materials, Stefano-Franscini-Platz 3, 8093 Zürich, Switzerland Empa ? Swiss Federal Laboratories for Material Science and Technology, Cellulose & Wood Materials, Überlandstrasse 129, 8600 Dübendorf, Switzerland; Munish Chanana: ETH Zürich, Institute for Building Materials, Stefano-Franscini-Platz 3, 8093 Zürich, Switzerland Empa ? Swiss Federal Laboratories for Material Science and Technology, Cellulose & Wood Materials, Überlandstrasse 129, 8600 Dübendorf, Switzerland Swiss Wood Solutions AG, Stefano-Franscini-Platz 3, 8093 Zürich, Switzerland

Resume : Continuous-flow biocatalysis has big potential as an efficient green alternative to established catalytic processes. It offers a wide variety of possible chemical transformation at moderate reaction conditions. However, most of these processes rely on the immobilization of enzymes on synthetic polymeric beads or membranes. These materials are unsustainable, as they are oil-based and cannot be recycled. Hence, the investigation of greener alternatives is demanded. Wood is a natural material with a complex anisotropic structure, which is dominated by micrometer-sized pores that are aligned along the tree?s growth direction. These pores, which are naturally designed for directed transport of liquids, make wood an interesting membrane material. Hence, we here present a wood-based biocatalytic flow-through reactor. Enzymes were immobilized inside the wood-structure by a two-step process, which relies on nanoparticle-mediated adsorption. The process is easy to execute, versatile, and completely water-based. The resulting wood-metal-enzyme hybrids offer excellent reusability and can be applied as biocatalytic flow-through reactors. Moreover, a two-step reaction cascade was executed in continuous flow. These bio-based biocatalytic membrane reactors can be a promising material for green processes.

R.4.16
14:55
Authors : Agnese Rabissi1-2, Alejandro Alonso-Díaz2, Jordi Floriach-Clark1, Montserrat Capellades2, Núria S. Coll2 , Anna Laromaine1*
Affiliations : 1. Institute of Material Science of Barcelona (ICMAB), CSIC, Campus UAB, Bellaterra, Barcelona, 08193, Spain. 2. Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, 08193, Spain. * lead presenter

Resume : Food security and the need to increase sustainably crop yields for a rapidly growing world population are among the greatest social and economic challenges of our century. The most extended agricultural practice to enhance crop yield is to increase host plant density, which in turn tends to increase the severity of plant diseases.1 Furthermore, globalization has contributed to the spreading of new pests into regions in which plants were not adapted, causing unaffordable agronomic losses. Most plant diseases occurring in agriculture are caused by fungal pathogens.2 Plant diseases caused by pathogenic bacteria are less prevalent; but their effects on agriculture are also devastating.3 In this work, we present an anti-bacterial and anti-fungal patch, which exploits the potential of smart-based nanomaterials as pesticides and silver nanoparticles (AgNPs) as anti-bacterial active component, improving the efficiency and environmental sustainability of pesticides application. In order to prevent the release of the NPs to the environment and their runoff loss during application, we attached the active silver nanoparticles to a bacterial cellulose (BC) matrix to obtain BC-AgNPs hybrid films. The bacterial cellulose matrix has similar chemical composition to the plant cellulose present in leaves but shows higher purity, crystallinity and water absorbance.4-5 We take advantage of the gel-like nature of the bacterial cellulose matrix to in situ synthesize and embed AgNPs. We evaluated the release and attachment of silver nanoparticles to a bacterial cellulose matrix and their in vitro anti-pathogenic properties against an array of plant pathogens with high agro-economical impact, such as the bacterium Pseudomonas syringae and the fungus Botrytis cinerea. These anti-bacterial and anti-fungal capacities were also assessed in vivo using the plant species Nicotiana benthamiana a close relative of tobacco and tomato; both widely used as a model organisms in plant biology.6 Insights of other applications on wound healing also will be provided. References (1) Sundström, J. F. et al. Future threats to agricultural food production posed by environmental degradation, climate change, and animal and plant diseases - a risk analysis in three economic and climate settings. Food Secur. 6, 201?215 (2014). (2) Agrios, G. N. Plant Pathology. Quinta edición. Academic Press. Nueva York. (2005). (3) V. Rajesh Kannan, K. K. B. Sustainable Approaches to controlling plant pathogenic bacteria. (2015). (4) Klemm, D. et al. Nanocelluloses: A new family of nature-based materials. Angew. Chemie - Int. Ed. 50, 5438?5466 (2011). (5) 5 Zeng, M., Laromaine, A. & Roig, A. Bacterial cellulose films: influence of bacterial strain and drying route on film properties. Cellulose 21, 4455?4469 (2014). (6)Enhancing Localized Pesticide Action through Plant Foliage by Silver-Cellulose Hybrid Patches A. Alonso-Díaz, J.i Floriach-Clark, J. Fuentes, M. Capellades, N. S. Coll, A. Laromaine; ACS Biomater. Sci. Eng., (2019), 5 (2), pp 413?419.

R.4.17
15:05
Authors : Antonina P. Naumenko
Affiliations : Taras Shevchenko National University of Kyiv, Faculty of Physics Volodymyrs’ka str. 64/13, 01601 Kyiv, Ukraine

Resume : Heterocyclic compounds play a vital role in the metabolism of all living cells. There are vast numbers of pharmacologically active heterocyclic compounds, many of which are a regular clinical use. Nitrogen and oxygen containing five member heterocyclic compounds have occupied enormous significance in the field of drug discovery process. The pharmacological potential of heterocycles containing the oxazolemoiety was studied, what has enabled the wide introduction of novel farmacological remedies in medicinal practice. As reference molecules we choose substituted 1,3-oxazoles that are an important class of heterocyclic compounds because of their wide spectrum of biological activities. These compounds exhibit a wide range of biological activities, including cytotoxicity, immunosuppression, multiple drug resistance pump inhibition, antibacterial and antiviral activities. They have shown themselves as powerful scaffold in drug design, especially, highly active anticancer agents. In addition, 1,3-oxazole derivatives are useful synthetic intermediates and can be used as diversity scaffolds in combinatorial medical chemistry. This report summarizes the results of the widest investigations of the relationship between the biological affinity and the electron structure of oxazole itself and its main derivatives, as well as comparing these results with the pharmacological testing. Here, the original fragment-to-fragment approach (Pharmacophor molecule-Biomolecule) is proposed for study the interaction between 1,3-oxazole derivatives (as a pharmachophor) and active center of biomolecule. The main characteristics of the electron structure (optimized molecular geometry, charge distribution, energies and shapes of molecular orbitals) were calculated by DFT/6-31 (d,p)/wB97XD method (package GAUSSIAN 09)

R.4.18
15:15
Authors : Chang Kyu Jeong
Affiliations : Division of Advanced Materials Engineering, Jeonbuk National University, Jeonju, Jeonbuk 54896, Republic of Korea

Resume : Elastic composite-based piezoelectric energy harvesting technology is highly desired to enable a wide range of device applications, including self-powered wearable electronics, robotic skins, and biomedical devices. Recently developed piezoelectric composites are based on inorganic piezoelectric fillers and polymeric soft matrix to take the advantages of both components. However, there are still limitations such as the weak stress transfer to piezoelectric elements and the poor dispersion of fillers in matrix. In this talk, a highly-enhanced piezocomposite energy harvesters (PCEH) is representatively developed using a three-dimensional (3D) interconnected electroceramic skeleton by mimicking and reproducing the sea porifera architecture (although the speaker will introduce other research examples and achievements). This new mechanically reinforced PCEH is demonstrated to resolve the problems of previous reported conventional piezocomposites, and in turn induces stronger piezoelectric energy harvesting responses. The generated voltage, current density and instantaneous power density of the biomimetic PCEH device reach up to ~16 times higher power output than that of conventional randomly-dispersed particle-based PCEH. This work broadens the further developments of high-output elastic piezocomposite energy harvesting and sensor application with biomimetic architecture.

R.4.19
15:30 16:00 Coffee Break, General Photo    
 
Frontier Research in Biomaterials and Nanomedicine FORUM Invited Presenters : Organizer/Chair Professor Peilin Chen (Academia Sinica, Taiwan)
16:00
Authors : Dar-Bin Shieh, DDS, DMSc
Affiliations : 1 Institute of Oral Medicine and Department of Stomatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan 2 International Institute of Macromolecular Analysis and Nanomedicine Innovations (iMANI), Center of Applied Nanomedicine, Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan, Taiwan.

Resume : We developed a type of zero-valent iron nanoparticle (ZVI NP) that exhibits selective anti-cancer potency through non-apoptotic cell death in several cancer types. In human head-and-neck cancers (HNC), we demonstrated that ZVI NPs selectively induce cancer ferroptosis yet spare the normal cells and presented strong efficacy in human cancer bearing rodent model without detectable systemic toxicity. Mitochondria and lysosome dependent pathways play critical roles in the ZVI NPs induced cancer cells death. Further investigation revealed that 10 ferroptosis associated gene panel may serve to predict their susceptibility to ZVI NPs treatment. Combination of ferroptosis inducers with ZVI could reverse ZVI therapeutic resistance. In addition, we also discovered that ZVI NPs inhibited the allograft growth of mouse Lewis lung carcinoma. Strikingly, multi-color fluorescent-immunohistochemistry demonstrated that the infiltrated M1 macrophages increased in tumor site whereas M2 macrophage located toward the tumor periphery after ZVI NPs treatment. ZVI also significantly down regulate PD-L1 in tumor infiltrating T cells. These results suggested that ZVI NPs inhibit tumor growth in vivo through both direct induction of cancer cell ferroptosis and modulation of tumor microenvironmental immune system towards anti-cancer phenotype.

R.5.1
16:30
Authors : Yu-Hsuan Chen, Hsuen-Li Chen, Dehui Wan
Affiliations : Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan (Yu-Hsuan Chen, Dehui Wan); Department of Material Science and Engineering, National Taiwan University, Taipei, Taiwan (Hsuen-Li Chen)

Resume : Nowadays, problems of energy crises and climate change pose a threat to human living. Therefore, developing eco-friendly cooling systems is an utmost issue to work on. Most of current cooling methods require energy to carry heat away. By means of passive radiative cooling, the system can radiate heat to outer space through the atmospheric transmission window between 8-13 ?m, thus lowering the temperature without any energy consumption. Some photonic solar reflector and thermal emitter, like HfO2, SiO2 and metamaterial film, can reflect incident sunlight while emitting radiation in the atmosphere windows. However, with its complicated fabrication and high cost, nanophotonic approach is hard to scale up to meet the requirements of commercial applications. Meanwhile, we focus on natural materials of great potential which are still not well-studied. Compared to the typically narrow Infrared (IR) emissivity peaks of artificial daytime cooling materials, the extremely broadband emissivity peak of the natural materials could cover two atmospheric windows (8-13 ?m and 16-25 ?m). Here, we present a study on silk cocoon, which is characterized by its 90% absorption at IR wavelengths and simple, well-studied fabrication process for different morphologies. We fabricate silk fibroin thin film with thickness ranging from 6 to 88 ?m by adjusting the concentration of silk fibroin solution. We observe the broadening of thickness leads to an increase in emissivity over IR wavelength regions, indicating thickness is the key factor to optical and radiative control. In addition to simulated spectra, optical constants of silk film are considered for further analysis. We also investigate the board band absorption and extinction coefficient at NIR wavelength. Throughout the IR wavelength, silk film exhibits a maximum absorbance of 92% at the thickness of 100 ?m. However, absorbance of silk film has positive correlation with thickness in solar spectrum region, resulting in high Psun performance. Therefore, thermal simulation is applied to find the lowest Tequ, at which the optimal thickness of silk film is located. Finally, we conduct temperature measurement on silk film practically to verify its daytime radiation cooling ability. With silk covering, the temperature of mobile phone decrease by 3.5 K, while the average absorbance of mobile phone is significantly enhanced to 94 % in atmospheric window.

R.5.2
16:45
Authors : Tzu-Ting Tseng,?, Yi-Ping Chen, *,?,?, Yun-Pu Chang,? Chung-Yuan Mou,?,? and Si-HanWu*,?,?
Affiliations : ?Department of Chemistry, National Taiwan University, Taipei 106, Taiwan ?Department of Chemistry, University of California Davis, California 95616, United States ?Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 110, Taiwan ?International Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan

Resume : Mesoporous silica nanoparticles (MSNs) is a promising nanocarrier for delivering anti-tumor drugs to cancer. Polyethylene glycol (PEG) has been linked to many nanocarriers to increase the dispersity, bioavailability, and circulation time of nanoparticles. However, on the other hand, PEGylation could limit the cellular uptake and endosomal escape of MSN, resulted in significant loss of activity of the delivery system. In this study, to overcome the ?PEG dilemma?, we synthesized pHR-MSN-PEG/DA@EPIs, in which pH-responsive (pHR) core-shell MSNs were utilized as nanocarriers, the anti-tumor drug epirubicin (EPI) was encapsulated by electrostatic interaction, and the amine-containing (DA) functional groups were introduced to control the loading and release of EPI. In vitro studies showed that the plain pHR-MSN-PEG/DA is non-toxic and the cleavage of PEG from carriers was achieved in response to an acidic environment, result in high efficiency of cellular uptake. In addition, pHR-MSN-PEG/DA@EPIs showed considerable cytotoxicity towards 4T1 tumor cells. For in vivo studies, pHR-MSN-PEG/DA showed excellent passive targeting behavior due to the enhanced permeability and retention (EPR) effect, and strong tumor inhibition effects in a chicken embryo chorioallantoic membrane (CAM) tumor model.

R.5.3
17:00
Authors : Si-Han Wu, Chung-Yuan Mou, and Yi-Ping Chen
Affiliations : Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 110, Taiwan

Resume : Overcoming the immunosuppressive tumor microenvironment is critical to realizing the potential of cancer immunotherapy strategies. Recent evidences are emerging to show that the cyclic di-guanylate (c-di-GMP), a molecular adjuvant, could induce the production of type I interferons (IFNs) via the stimulator of interferon genes (STING) dependent pathway in antigen presenting cells (APC), leading to enhance the tumor immunogenicity. However, the efficacy of negatively charged c-di-GMP may be limited by some inherent shortcomings, including the poorly membrane permeable, rapid clearance and the inefficiency of cytosolic delivery. Hence, we attempt to develop an alternative ?in situ vaccination? approach based on nanotechnology to initiate an antitumor immune response. In this study, PEGylated RITC fluorescent mesoporous silica nanoparticles (MSN) with a positively charged molecule (N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride, TA) by co-condensation was synthesized to form RMSN-PEG/TA. The characteristics of nanoparticles were determined by transmission electron microscopy (TEM), dynamic light scattering (DLS) and nitrogen adsorption-desorption isotherm. The anionic c-di-GMP was loaded into cationic RMSN-PEG/TA via electrostatic interactions (c-di-GMP@RMSN-PEG/TA), showing the loading amount of c-di-GMP on c-di-GMP@ RMSN-PEG/TA was around 3 wt%. RAW264.7 cells treated with c-di-GMP@ RMSN-PEG/TA obvious increased the production of IL-6, IL-1?, and IFN-? analyzed by real-time PCR, as well as the expression level of phospho-STING (Ser365) protein investigated by western blot. The mouse 4T1 breast tumor-bearing Balb/c mice received injection of c-di-GMP@ RMSN-PEG/TA revealed dramatically tumor growth inhibition accompanied by the infiltration of activated CD11c+ dendritic cells and CD4+ T cells at the tumor site detected by flow cytometry. We validate this in situ vaccination by STING pathway activation provides an attractive therapeutics for breast cancer, highlighting its potential to improve clinical outcomes of cancer immunotherapy.

R.5.4
17:15
Authors : Tai-Wei Feng, Jui-Ting Hsiao and Ming-Fa Hsieh
Affiliations : Department of Biomedical Engineering, Chung Yuan Christian University, Taiwan

Resume : Introduction The most common cancer in women is breast cancer and it continues to affect millions of people worldwide. Survival rate increases with early detection but diagnostic procedures may also put patients at risk because of radiation exposure. Development of alternatives such as near-infrared diagnostics may be the key to this problem. Fluorescent dyes may be used but due to their intrinsic characteristics, modification or encapsulation must be done to make it biocompatible. With this, biopolymers may be employed as carriers or encapsulating agents. Experiment Synthesis of polyester-based amphiphilic block copolymers was carried out by ring-opening polymerization reaction. The critical micelle concentration of the copolymers were measured followed by the encapsulation of 2QMEH, a two-photon fluorescent dye. The optimal copolymer for 2QMEH encapsulation showing a loading efficiency of 68.6% was selected. Results and Discussion Encapsulation of 2QMEH gave a two-photon absorption (TPA) cross section of around ~55-120 GM at 820 nm. This is significantly greater than the threshold value for biological applications showing a good potential for in vivo bio-imaging to be used together with near-infrared for breast cancer diagnosis. Acknowledgements: The authors would like to thank the Ministry of Science and Technology of Taiwan for the financial support under grant number MOST 108-2119-M-033-002. References 1) M.L. Gou, X.L. Xheng, K. Men, J. Zhang, L. Zheng, X.H. Wang, F. Luo, Y.L. Zhao, X. Zhao, Y.Q. Wei, Z.Y. Qian, J. Phys. Chem. B., 113, 39 (2009). 2) M. Drobizhev, S. Tillo, N.S. Makarov, T.E. Hughes, A. Rebane, J. Phys. Chem. B., 113, 4 (2009).

R.5.5
17:30
Authors : Jau-Ye Shiu, Zhe Lin, Lina Aires, and Viola Vogel
Affiliations : ETH Zurich, department of health science and technology

Resume : Our ability to control cell behavior by properly engineered materials and microenvironments is tightly coupled to understanding the mechanisms of cell-matrix interactions. Anisotropy of extracellular matrix (ECM) drives cell alignment and directional migration during processes like development and wound healing, but also in cancer cell migration and invasion. So far, migrational persistence whether on flat isotropic or anisotropic surface patterns and fibers was mostly studied as local phenomenon asking how specific integrins are responsible for the recognition of the spatial ECM cues. Yet, cell alignment and directional migration in response to external ECM cues requires signal integration across length scales. By producing patterned 2 µm ECM stripes, which lead to cell alignment, and testing the previously described pan-integrin null fibroblast cells, we indeed observed that cell alignment and directional migration on patterned stripes were lost when ?1 integrin was absent. By combining nanopillar arrays with printed cell-adhesive fibronectin (FN) stripes, we could probe subcellular force distributions at submicron resolution. While it was previously recognized that the ?v- and ?1-class integrin signaling pathways are coupled, via myosin II contractility, we discover here that myosin III coupling of these integrin signaling pathways requires a stiff cell nucleus. Importantly, directional migration along adhesive patterned stripes was also impaired for lamin A/C knockout cells, incapable of forming an actin cap and restored upon lamin A/C rescue. Together, our data suggest that ?1 integrin is required for the recognition of spatial ECM cues and that force transmission to the nucleus via lamin A/C is essential for subsequent directional migration.

R.5.6
 
ROUND TABLE DISCUSSION on FRONTIER RESEARCH : invited 7en minutes Key Speech on Frontier Reserch Organizer/Chair Dr. Donata Iandolo (Ecole des Mines de Saint-Etienne, University of Lyon, Universite Jean Monnet, INSERM, France) (17.45-18.30)
17:45
Authors : Antonina P. Naumenko
Affiliations : Taras Shevchenko National University of Kyiv, Faculty of Physics Volodymyrs’ka str. 64/13, 01601 Kyiv, Ukraine

Resume : Cisplatin (cis-Pt) is a complex of platinum which widely used as an anticancer drug. Transplatin (trans-Pt) has the same chemical formula, but different positions of atoms. Cisplatin connects to DNA in cancer cells and stops DNA replication, namely making cross-links between guanine bases. Transplatin is less reactive to DNA but also demonstrate cytotoxicity effect. In this work we make an attempt to connect the differences in the biological activity of mentioned structures with the data obtained by optical spectroscopy methods. The UV-vis spectra as well as fluorescence and phosphorescence spectra of water solutions of cis-Pt and trans-Pt were investigated at temperatures 300 and 77 K. Quantum chemical calculations were used for correct interpretation of obtained results. We also characterized these compounds using such powerful method as Raman spectroscopy.

R.RT3.1
17:45
Authors : Jing Yu1, Minglun Li1, Xin Xu1, Jilei Wang1, Yan Cheng1
Affiliations : 1 School of Materials Science and Engineering, Nanyang Technological University, Singapore

Resume : The unique functionalities of polyelectrolyte brushes depend on several types of specific interactions, including solvent structure effects, hydrophobic forces, electrostatic interactions, and specific ion interactions. Subtle variations in the solution environment can lead to conformational and surface structural changes of the polyelectrolyte brushes, which is discussed mainly from a surface interaction perspective in this review. We highlight the use of these surface-grafted polymer films to create functional surfaces for various applications, including non-fouling surfaces, boundary lubricants, as well as stimuli-responsive surfaces. Based on the SFA and AFM methods, the impact factors for constraining the lubricity of polyelectrolyte brushes have been investigated. Experimental results reveal that the charged polymer chains and the osmotic pressure of the counterions can lead to wear resistance and low friction in monovalent salt solution. However, the lubrication can break down in the presence of multivalent counterions, even at very low concentrations, resulting from electrostatic bridging and brush collapse between chains from apposing surfaces and changes in surface topology. This study helps to give some insight into the interchain interactions contained in polyelectrolyte brushes but also can promote their application in many technical, medical, physiological, and biological areas. Additionally, we investigated the structure and antifouling properties of surface tethered zwitterionic peptide monolayers with different peptide chain lengths and charge distributions using a combination of surface plasma resonance (SPR), atomic force microscopy (AFM), and all atomistic molecular dynamics (MD) simulation techniques. Our results demonstrate that longer zwitterionic peptides exhibit better antifouling performance. The patchy charge distributions of the positive and negative charges in the peptides, although affecting the structure of the peptide molecules, do not significantly change the antifouling properties of the peptide monolayers.

R.RT3.2
17:45
Authors : Chiung Wen Kuo, Feby Wijaya Pratiw, Peilin Chen
Affiliations : Research Center for Applied Sciences, Academia Sinica, Taiwan

Resume : In this lecture, we will discuss the use of surface-modified nanoparticles such as quantum dots and mesoporous nanoparticles (MSNs) for bio-imaging. The applications of surface-modified nanoparticles for pH sensing will be first introduced. We will present two ratiometric pH sensor designs using water-soluble, dual-emission, Mn2+-doped quantum dots (Qdots) decorated with D-penicillamine (DPA-MnQdots) and mesoporous silica nanoparticles (MSNs) modified with two different pH-sensitive dyes, fluorescein isothiocyanate (FITC) and Oregon green (OG), and a reference dye, rhodamine B isothiocyanate (RITC). The behavior of these surface-modified nanoparticles in cells will be discussed. For in vivo imaging, we will present the study of real-time circulating tumor cells (CTCs) and cancer stem cells (CSCs) imaging in the bloodstreams of living animals using multi-photon microscopy and antibody-conjugated quantum dots. We have developed a cancer model for noninvasive imaging wherein pancreatic cancer cells expressing fluorescent proteins were subcutaneously injected into the earlobes of mice to form solid tumors. When the cancer cells broke from the solid tumor, CTCs with fluorescent proteins in the bloodstream at different stages of development could be monitored noninvasively in real-time.

R.RT3.3
17:45
Authors : Csaba Balazsi
Affiliations : Centre for Energy Research, Konkoly ? Thege M. str. 29-33, 1121 Budapest, Hungary

Resume : Ceramic structures at the nanometre range have been proven to have improved properties and characteristics that differ from their bulk, allowing for opportunities in novel technological applications. Ceramic layers with some advantages, including a very high surface-to-volume and aspect ratio can be processed by cheap and quick method, that is electrospraying. This presentation reviews results on the electrospinning and electrospraying of various bioceramic layers starting from biogenic raw materials and their potential applications in the biomedical field.

R.RT3.4
17:45
Authors : Joana Lopes1,3, Gonçalo Oliveira1,3, Marta Rocha1, Fábio Mendes1, Pedro Duarte2, André Santos2, Cláudia Nunes1,3, Paula M. Vilarinho2, Paula Ferreira2, Manuel A. Coimbra3, Idalina Gonçalves1
Affiliations : 1CICECO – Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal 2CICECO – Aveiro Institute of Materials, Department of Materials and Ceramic Engineering, University of Aveiro, 3810-193, Aveiro, Portugal 3LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal

Resume : The huge amount of landfilled plastic and agrofood wastes derived from the extensive trade in agrofood sector, the short shelf-life of fresh foodstuffs, and the plastic packaging materials give rise to harsh environmental issues. Aiming to overcome these constraints, in this work, the feasibility of using agrofood byproducts on the development of active biobased materials was studied. As cases of study potato and coffee roasting byproducts were used as raw materials. The influence of phenolic compounds recovered from potato peels and coffee silverskin dosage on physicochemical, mechanical, and antioxidant properties of potato starch (recovered from potato washing slurries) films was studied. In addition, the processability of potato starch/coffee silverskin-based formulation by a 3D printing technology (FDM) was assessed. Both potato peel-phenolic compounds and coffee silverskin increased the flexibility, humidity tolerance, and antioxidant capacity of pristine potato starch films. The potato starch/coffee silverskin-based formulation was efficiently extruded as 3D printer filaments and further used to print a prototype. This work confirms the suitability of considering potato and coffee roasting byproducts as a source of biomolecules of interest for the development of antioxidant biobased formulations that can bring benefits for both agrofood and plastic industries while moving forward to the additive manufacturing processes. Acknowledgments: Thanks are due to the University of Aveiro and FCT/MCTES for the financial support of QOPNA research Unit (FCT Ref. UID/QUI/00062/2019) and of CICECO-Aveiro Institute of Materials (FCT Ref. UIDB/50011/2020 & UIDP/50011/2020). The authors acknowledge to POTATOPLASTIC project (POCI-01-0247-FEDER- 017938), financed by FEDER through POCI, “Isolago – Indústria de Plásticos, S. A.”, the project leader, “A Saloinha, Lda.” for providing potato byproducts, and to FEB – Cafés, S. A. for supplying coffee silverskin. The costs resulting from the FCT hiring is funded by national funds (OE), through FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. FCT is also thanked for the Investigator FCT program (PF, IF/00300/2015), for the Individual Call to Scientific Employment Stimulus (IG, CEECIND/00430/2017), and for the grants ref. SFRH/BPD/104712/2014 (IG), SFRH/BD/136804/2018 (JL), and SFRH/BPD/100627/2014 (CN).

R.RT3.5
17:45
Authors : Agnese Rabissi1-2, Alejandro Alonso-Díaz2, Jordi Floriach-Clark1, Montserrat Capellades2, Núria S. Coll2 , Anna Laromaine1*
Affiliations : 1. Institute of Material Science of Barcelona (ICMAB), CSIC, Campus UAB, Bellaterra, Barcelona, 08193, Spain. 2. Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, 08193, Spain. * lead presenter

Resume : Food security and the need to increase sustainably crop yields for a rapidly growing world population are among the greatest social and economic challenges of our century. The most extended agricultural practice to enhance crop yield is to increase host plant density, which in turn tends to increase the severity of plant diseases.1 Furthermore, globalization has contributed to the spreading of new pests into regions in which plants were not adapted, causing unaffordable agronomic losses. Most plant diseases occurring in agriculture are caused by fungal pathogens.2 Plant diseases caused by pathogenic bacteria are less prevalent; but their effects on agriculture are also devastating.3 In this work, we present an anti-bacterial and anti-fungal patch, which exploits the potential of smart-based nanomaterials as pesticides and silver nanoparticles (AgNPs) as anti-bacterial active component, improving the efficiency and environmental sustainability of pesticides application. In order to prevent the release of the NPs to the environment and their runoff loss during application, we attached the active silver nanoparticles to a bacterial cellulose (BC) matrix to obtain BC-AgNPs hybrid films. The bacterial cellulose matrix has similar chemical composition to the plant cellulose present in leaves but shows higher purity, crystallinity and water absorbance.4-5 We take advantage of the gel-like nature of the bacterial cellulose matrix to in situ synthesize and embed AgNPs. We evaluated the release and attachment of silver nanoparticles to a bacterial cellulose matrix and their in vitro anti-pathogenic properties against an array of plant pathogens with high agro-economical impact, such as the bacterium Pseudomonas syringae and the fungus Botrytis cinerea. These anti-bacterial and anti-fungal capacities were also assessed in vivo using the plant species Nicotiana benthamiana a close relative of tobacco and tomato; both widely used as a model organisms in plant biology.6 Insights of other applications on wound healing also will be provided. References (1) Sundström, J. F. et al. Future threats to agricultural food production posed by environmental degradation, climate change, and animal and plant diseases - a risk analysis in three economic and climate settings. Food Secur. 6, 201?215 (2014). (2) Agrios, G. N. Plant Pathology. Quinta edición. Academic Press. Nueva York. (2005). (3) V. Rajesh Kannan, K. K. B. Sustainable Approaches to controlling plant pathogenic bacteria. (2015). (4) Klemm, D. et al. Nanocelluloses: A new family of nature-based materials. Angew. Chemie - Int. Ed. 50, 5438?5466 (2011). (5) 5 Zeng, M., Laromaine, A. & Roig, A. Bacterial cellulose films: influence of bacterial strain and drying route on film properties. Cellulose 21, 4455?4469 (2014). (6)Enhancing Localized Pesticide Action through Plant Foliage by Silver-Cellulose Hybrid Patches A. Alonso-Díaz, J.i Floriach-Clark, J. Fuentes, M. Capellades, N. S. Coll, A. Laromaine; ACS Biomater. Sci. Eng., (2019), 5 (2), pp 413?419.

R.RT3.6
17:45
Authors : S. Yu. Turishchev1, D. Marchenko2, V. Sivakov3, E.A. Belikov1, O.A. Chuvenkova1, E.V. Parinova1, D.A. Koyuda1, R.G. Chumakov4, A.M. Lebedev4, T.V. Kulikova1, A.A. Berezhnoi1, I.V. Valiakhmedova1, E.V. Preobrazhenskaya5 and S.S. Antipov1,5,6
Affiliations : 1Voronezh State University, 394018, Voronezh, Russia 2Helmholtz-Zentrum Berlin, 12489, Berlin, Germany 3Leibniz Institute of Photonic Technology, 07745, Jena, Germany 4National Research Center "Kurchatov Institute", 123182, Moscow, Russia 5Institute of cell biophysics RAS, 142290, Pushchino, Russia 6Immanuel Kant Baltic Federal University, 236016, Kaliningrad, Russia

Resume : The combination of a few nanometers inorganic particles with nature-like objects can play a crucial role in engineering of novel low-dimensional hybrid materials. One of the convenient objects for such kind of technology development is E.coli bacterial culture. The surface properties play an important role for such systems. PhotoEmission Electron Microscopy (PEEM) can play a key-role by providing the powerful ability to perform chemically sensitive small spot spectromicroscopy surface analysis at one time. The crucial point is bio-objects stability under special conditions of surface sensitive vacuum experiments. X-ray photoelectron spectroscopy (XPS) and Scanning Electron Microscopy (SEM) control studies were performed before and after PEEM experiments with E.coli cells. PEEM images were collected under Hg lamp irradiation and with the use of tunable high intensive synchrotron light. Obtained results demonstrate a possibility of effective PEEM bioimaging of the E.coli cells under ?hard? conditions. The surface topology of single bacteria has been detected by PEEM that well correlates with the SEM studies results. Only partial bacteria shell damage have been shown. Our observation strongly suggests that PEEM spectromicroscopy surface analysis can be applied up to a single E.coli cell structure and composition studies without significant bioobject destruction. The study was supported by Russian Science Foundation (Pr. 19-72-20180).

R.RT3.7
17:45
Authors : Donata IANDOLO
Affiliations : INSERM U1059, Campus Santé Innovations, Saint-Priest-en-Jarez, France

Resume : Bioelectricty is reported to be one of the key regulators of morphogenesis in vivo and electrical potentials have been reported to influence a number of physiological processes. Bioelectronics materials and devices are able to interact with living systems (e.g., tissues, organs, cells). They are indeed able to translate an ionic signal into an electronic readout and they are also able to deliver stimuli influencing ion movements therefore affecting specific target functions (e.g., tissue regeneration).

R.RT3.8
17:45
Authors : Dr., Docent Oleksandr .Ivanyuta
Affiliations : Taras Shevchenko National University of Kyiv,64/13, Volodymyrska Str., Kyiv, 01601, Ukraine iva@univ.net..ua

Resume : A biosensor based on collagen composite biopolymers nanofibers is found to be effective in identifying physical influences. Biopolymer nanofibers were made by electrospinning method. After optimizing the synthesis parameters, nanofibers biopolymers were obtained, averaged 50-100 nm in diameter, and then modified on the surface of the working electrode. Effect of the intensity of the transducer buffer and the working potential of the current response. The nanofilament-modified electrode to the temperature is optimized to obtain the maximum current response [1]. Effect of the intensity of the transducer buffer and the working potential of the current response. The nanofilament-modified electrode to the temperature is optimized to obtain the maximum current response. The results revealed that the temperature of the collagen composite biopolymer nanofibers had the advantages of rapid reaction, excellent reproducibility, high stability and showed a nonlinear reaction in the dynamic range from 273 K to 373 K with a detection limit of 0.1 K and a high sensitivity of 1 µA·mM−1· cm−2. The proposed strategy based on collagen composite biopolymer nanofibers can be extended for the development of other enzyme-based biosensors. [1] O.Dubok, O.Shynkaruk, E.Buzaneva, Lanthanides oxides usage to increase radiopaque of bioactive ceramics, Funct. Mater. 2013; 20 (2): 172-179. http://dx.doi.org/10.15407/fm20.02.172

R.RT3.9
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Frontier Research in Biomaterials and Nanomedicine FORUM Invited Presenters : Organizer/Chair Professor Peilin Chen (Academia Sinica, Taiwan)
08:30
Authors : Peilin Chen
Affiliations : Research Center for Applied Sciences, Academia Sinica, Taiwan.

Resume : In this study, we employed a novel one-step electrospinning process to fabricate poly(ethylene oxide) (PEO)/poly(3,4-ethylenedioxythiophene): polystyrenesulfonate (PEDOT:PSS) core/shell nanofiber structures with improved water resistance and good electrochemical properties. We then integrated a biocompatible polymer coating with three-dimensional (3D) PEDOT-based nanofiber devices for dynamic control over the capture/release performance of rare circulating tumor cells (CTCs), as well as the label-free detection by using organic electrochemical transistors (OECTs). The detailed capture/release behavior of the circulating tumor cells was studied using an organic bioelectronic platform comprising PEO/PEDOT:PSS nanofiber mats with 3 wt % (3-glycidyloxypropyl)trimethoxysilane as an additive. We have demonstrated that these nanofiber mats deposited on five-patterned indium tin oxide finger electrodes are excellent candidates for use as functional bioelectronic interfaces for the isolation, detection, sequential collection, and enrichment of rare CTCs through electrical activation of every single electrode. This combination behaved as an ideal model system displaying a high cell-capture yield for antibody-positive cells while resisting the adhesion of antibody-negative cells. Taking advantage of the electrochemical doping/dedoping characteristics of PEDOT:PSS materials, the captured rare cells could be electrically triggered release through the desorption phenomena of PLL-g-PEG-biotin on the device surface. More than 90% of the targeted cancer cells were captured on the 3D PEDOT-based nanofiber microfluidic device; over 87% of captured cancer cells were subsequently released for collection; approximately 80% of spiked cancer cells could be collected in a 96-well plate. For the OECT design, it was demonstrated for monitoring CTC-capture performance and identifying cancer cell phenotypes. This 3D PEDOT-based bioelectronic device approach appears to be an economical route for the large-scale preparation and detection of systems for enhancing the downstream characterization of rare CTCs.

R.5.7
08:55
Authors : Fuyuhiko Tamanoi
Affiliations : Institute for Integrated Cell-Material Sciences, Institute for Advanced Study, Kyoto University

Resume : Our attempt to develop a new type of radiation therapy by using gadolinium-loaded nanoparticles and synchrotron-generated monochromatic X-rays will be discussed.

R.5.8
09:20
Authors : Michihiro Nakamura
Affiliations : Department of Organ Anatomy & Nanomedicine, Yamaguchi University Graduate School of Medicine

Resume : Medical applications of multifunctional nanoparticles are expected to be one of the most important possibilities in innovative medicine. Organosilica nanoparticles are novel nanomaterials that are prepared from a single organosilicate coupling agent (organotrialkoxysilane) such as 3-mercaptopropyltrimethoxysilane. Organosilica nanoparticles are both structurally and functionally different from typical silica nanoparticles (inorganosilica nanoparticles) prepared from tetraalkoxysilane. The organosilica nanoparticles contain both interior and exterior functionalities such as mercaptopropyl residue as prepared. The organosilica nanoparticles allow for facile surface and internal functionalization, offering new opportunities to create multifunctionalized nanoparticles. Over the last two decades, research on the internal functionalization of organosilica nanoparticles has evolved. Various sizes of fluorescent organosilica nanoparticle containing various types of fluorescent dye including near infrared (NIR) dye can be prepared using a one-pot synthesis. In addition, functional fusions of organosilica nanoparticles and other functional nanoparticles such as quantum dots, gold nanoparticles, and iron oxides are possible based on organosilica particles technology. These multifunctionalized organosilica nanoparticles are useful for various imaging techniques such as in vivo imaging, cell labeling, time-lapse fluorescent imaging, and multimodal imaging. Multifunctionalized organosilica nanoparticles have high potential to create novel imaging systems and provide novel information of cell characteristics and functions. In recent year, we have launched additional research on surface functionalization of organosilica nanoparticles using biomolecules and polymers. Surface-functionalized organosilica nanoparticles revealed various alterations of the interaction with cells including tumor cells and macrophages. We applied multifunctionalized NIR organosilica nanoparticles to tumor-bearing mouse. The particles showed an accumulation on tumor tissue on NIR in vivo imaging, and damaged tumor cells by using photodynamic effect. Imaging and therapy using multifunctionalized organosilica nanoparticles allow for nano-theranostics.

R.5.9
09:45
Authors : Mi Hyeon Cho, Yan Li, Hyunjin Kim, Yongdoo Choi
Affiliations : National Cancer Center

Resume : My laboratory has been developing various types of activatable and dual-targeted photosensitizing agents as smart theranostics for selective near-infrared fluorescence imaging and photodynamic therapy (PDT) of cancers and inflammatory diseases. Recently a fucoidan-based theranostic nanogel (CFN-gel) consisting of a fucoidan backbone, redox-responsive cleavable linker, and photosensitizer was developed to achieve activatable near-infrared fluorescence imaging of tumor sites and an enhanced PDT to induce the complete death of cancer cells. A CFN-gel has nanomolar affinity for P-selectin and VEGF. Moreover, a CFN-gel is non-fluorescent and non-phototoxic upon its systemic administration due to the aggregation induced self-quenching in its fluorescence and singlet oxygen generation. After internalization into cancer cells and tumor neovascular endothelial cells, its photoactivity is recovered in response to the intracellular redox potential, thereby enabling selective near-infrared fluorescence imaging and an enhanced PDT of tumors. It also provides a significant antitumor effect in the absence of light treatment in vivo. Our study indicates that a fucoidan-based theranostic nanogel is a new theranostic material for imaging and treating cancer with high efficacy and specificity.

R.5.10
10:10 Coffee Break    
10:30
Authors : I-Chun Chen, Ting-Yu Lu, Jiashing Yu
Affiliations : National Taiwan University, Taiwan

Resume : The native tissues are complex structures consisting of different cell types, extracellular matrix materials, and biomolecules. Traditional tissue engineering strategies have not been able to fully reproduce biomimetic and heterogeneous tissue constructs because of the lack of appropriate biomaterials and technologies. However, recently developed three-dimensional bioprinting techniques can be leveraged to produce biomimetic and complex tissue structures. To achieve this, multicomponent bioinks composed of multiple biomaterials (natural, synthetic, or hybrid biomaterials), different types of cells, and soluble factors have been developed. In addition, advanced bioprinting technologies have enabled us to print multimaterial bioinks with spatial and microscale resolution in a rapid and continuous manner, aiming to reproduce the complex architecture of the native tissues. In this study, we developmented a new formulation of bio-ink which is based on methacrylated keratin and methacrylated glycol chitosan. The feasibility of this bioink was tested with human adipose-derived stem cell toward specific differentiation conditions.

R.5.11
10:50
Authors : Hideaki Yamamoto1, Takuma Sumi1, Shigeo Sato1, Ayumi Hirano-Iwata1,2
Affiliations : 1. Research Institute of Electrical Communication, Tohoku University, Sendai, Japan; 2. WPI-Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, Sendai, Japan.

Resume : Nerve-cell culture takes an irreplaceable role in molecular and cellular neuroscience. However, its use in studies at the systems level has been limited due to the substantial difference between in vivo in vitro network dynamics. The dynamics is affected by the difference in the intercellular connectivity (Yamamoto et al., Sci. Adv. 4, eaau4914 (2018)), as well as by the excessively strong excitatory synapses in cultured neurons. Recently, a study was reported that stiff scaffolds enhance the strengths of excitatory synapses in cultured hippocampal neurons (Zhang et al., Sci. Rep. 4, 6215 (2014)). As neuronal cultures are generally performed on polystyrene or glass, which is approximately 10^6 to 10^7 times stiffer than the brain tissue, we hypothesized that the in vitro artifact in excitatory synaptic strength can be reduced by using a scaffold that mimics the elasticity of the brain tissue. Here we employed an ultrasoft silicone elastomer, whose elastic modulus resembles that of the brain tissue (~0.5 kPa), as a scaffold for culturing rat cortical neurons. We investigated the effect of the biomimetic elasticity on the strength of excitatory synapses and the spontaneous network activity (Sumi et al., arXiv 1912.05050 (2019)). We found that the amplitude of excitatory postsynaptic currents was smaller on softer scaffolds. Although globally synchronized bursting activity in cortical cultures were still observed when the cells were grown on the ultrasoft substrate, neuronal correlation was significantly reduced as compared to the cultures on stiffer (>14 kPa) substrates. In the latter half of the talk, we will show how the multielectrode array devices with the ultrasoft cell-device interface can be fabricated by taking advantage of additive manufacturing technologies, such as inkjet printing (Yamamoto et al., Adv. Biosys. 3, 1900130 (2019)). Our device employs 3D micropillar electrodes, which can be fabricated relatively easily by inkjet printing. Such devices facilitate the stimulation and recording of cultured neuronal networks grown on biomimetic scaffolds, for both basic research and pharmacological studies.

R.5.12
11:10
Authors : Shih-En Chou, Ji-Yen Cheng
Affiliations : Research Center for Applied Sciences, Academia Sinica Taiwan

Resume : Cell adhesion is a requisite stage in a number of physiological and pathological processes, such as cell differentiation, immune responses, and tumor metastasis. The process of cell adhesion is composed of three steps, cell-substrate contact, cell spreading, and cytoskeleton reorganization. Conventional adhesion assays, such as colorimetric and fluorometric detection, are time-consuming and insensitive. Tracking of cell morphological changes is often difficult to quantify. In the past decades, surface plasmon resonance (SPR) is widely applied in biosensing technology due to the rapid, real-time and label-free characteristics. Here, we developed a novel metal nanoslit-based biosensor with a detection platform of transmissive surface plasmon resonance (t-SPR). The platform can determine cell adhesion by Fano resonance signals, which are changed during cell binding to the nanoslit. Therefore, we can simultaneously analyze the focal adhesion and cell spreading through the spectral peak and dip of the Fano resonance. The peaks and dips reflect the long- and short-range cellular changes, respectively. We previously examined the features of cell adhesion and found that this method has great potential for estimating the thickness of adherent cells and the metastatic potencies of lung cancer cells. Metastasis, a complicated process in which cancer cells spread from a primary tumor to distant sites via the circulatory system, is the main cause of death in cancer patients. In light of this fact, we are now screening commercial drugs like ion-channel inhibitors and G protein-coupled receptors (GPCR) compounds which are related to cell adhesion and might involve in metastasis. The screening could reveal candidates which may be used as anti-metastasis drugs.

R.5.13
11:30
Authors : Daisuke Miyoshi, Kazuki Kohata, Wataru Sugimoto, Katsuhiko Itoh, Keiko Kwauchi
Affiliations : Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, 650-0047, Japan

Resume : Molecular environments inside cells are surprisingly crowded with numerous number of biomolecules. About 40 percent of the cell volume is occupied by biomolecules. Biochemical reactions are subject to be temporally, spatially, and specifically controlled. From biochemical and biophysical point of views, it is incredible that selective interactions and specific functionalization of biomolecules in the temporal and spatial-specific manner can be achieved under the complex molecular crowding environments. One of the key futures to achieve the specific interaction of biomolecules is collective biomolecular behavior. In recent years, biomolecular localization and compartmentation systems using droplets via liquid-liquid phase separation (LLPS) inside cells, become one of the most hot research topics in biology. Droplets are temporarily formed, and their formation is reversible and responsive to various external signals, which are in contrast with aggregation of biomolecules which is generally irreversible. In this presentation, we will show a model system of a droplet in a test tube, in which we use nucleic acids (RNA and DNA) which forms G-quadruplexes and arginine-rich peptides which do not have any stable structure. By mixing these two components, a rapid LLPS was observed. It was suggested that the G-quadruplex structure is critical for undergoing LLPS. In the talk, we would like to discuss property of the droplet as a biomaterial.

R.5.14

Symposium organizers
Emmanuel STRATAKISInstitute of Electrnic Structure and Laser (IESL)

Foundation of Research and Technology Hellas (FORTH) and University of Crete, Nikolau Plastira 1000, Voutes, Heraklion, Crete

+30 2810 3912 74
stratak@iesl.forth.gr
Eugenia BUZANEVATaras Shevchenko National University of Kyiv

NASU “Physical and Chemical Material Science Centre”, Volodymyrs'ka Str. 64/13, 01601 Kyiv, Ukraine

+38 044 294 26 22
emrs@univ.kiev.ua
Insung S. CHOI (Main)The Center for Cell-Encapsulation Research, KAIST

Dep. of Chemistry and Dep. Bio and Brain Engineering - 281, Daejeon 34141, Korea

+82 42 350 2880
ischoi@kaist.ac.kr
Peter SCHARFFTechnical University of llmenau

Institute of Chemistry and Biotechnology, Weimarer Strasse 25, 98693 Ilmenau, Germany

+49 36 77 69 3603(04)
peter.scharff@tu-ilmenau.de
Thomas J. WEBSTER Northeastern University

Department of Chemical Engineering - Center Advanced Materials Research - 313 Snell Engineering Center - Boston, IMA 02115, USA

+1 617 373 6585
websterthomas02@gmail.com