preview all symposia

LAYERED, HYBRID AND BIOMATERIALS

E

Bioinspired and biointegrated materials as new frontiers nanomaterials VIII

Following the success of the symposia I - VI due to the reports and discussions on  rapidly development  bioinspired, biomimetic technologies for next generation  biomedical nano – materials, - systems, - robotic devices,  the symposium VII  is aimed to give overview of  recent development for fundamentals of nanotechnologies for biomedical engineering multifunctional materials in biomedical healthcare field, environmental control and security. Newest nanotechnologies and bio - materials, - systems, - robotic devices fields which determine developing biomimetic cells and skin, bone tissue engineering, remodeling ones and adaptation to a regeneration of neural systems using created implantable bionic systems.

Scope:

The symposium will cover the frontiers on the engineering, researching, molecular scale characterization multifunctional  biomolecular biosensor systems in medical and environmental researches and bio - photonics, - electronics, - magnetic molecular systems, bioimmobilized NPs as nanorobots in vivo applications using bio – inspired, mimetic, templated by biomolecules ( virus, marine plants proteins, pigments) inorganic nanoparticles for the quantum dots nanosystems, bioinspired composite  materials... The design, engineering of these materials are aimed to obtain the properties which respond to external, biologically compatible stimuli (physical, chemical, biological) and to electronic, photonic, magnetic nanosystems. And next step is transferring from nano to macro materials for regenerative medicine of bones and teeth (stem cells regenerative orthopedic and dental medicine) for example, and  engineering of multifunctional biointerfaces and biotemplating. The symposium will bring together researchers from chemical, physical sciences and bio - science and – nanotechnology biomaterials for nanomedicine and engineering bio - electronic, - photonic, - magnetic nanosystems to discuss the latest advancements.

Proposed subjects for discussions at this event have actuality for Investigators of the EU MPNS, MP COST Actions on 2015 – 2018, for example, the Action MP 1005 ”From nano to macro biomaterials (design, processing, characterization, modeling and applications to stem cells regenerative orthopedic and dental medicine - NAMABIO)

A special Young Researcher session for ADVANCED Researchers (Post-Graduate, PhD and graduate students’ talks) is planned at the symposium’s first day on 16.30 – 18.00.                                   

Thank you in advance for your special message – asking to include your submitted abstract for keynote presentation at this session. 

Contact us nanobiotech@bigmir.net.

Hot topics to be covered by the symposium:

  • from biological supramolecular materials to bio – inspired and  - mimetic material synthesis;                    
  • bioinspired synthesis of inorganic  nanoparticles (NPs), systems;  
  • bio-hybrid biomedical nanomaterials: biosynthesized, bioimmobilized and biointegrated inorganic nanoparticles, carbon and bioimmobilized carbon supramolecules;   
  • 3D molecular imprinting biological cell structures and biomimetics ones as scaffolds in tissue engineering  
  • electronic, photonic and magnetic functions of  biosupramolecules (nucleic acids, virus, marine plants proteins, pigments) and mimetic analogs: adaptation to human systems functions for biomedical nano – systems, -  robotic devices designing, for example,  molecular robot - DNA motor “robotic” molecule into living cell; Special – Neuroelectronics.                                           
  • biosensing characterization for medical and environmental biotechnologies;                                
  • biomimetic analogs functions in compare to bionic functions for natural systems.

Documentation

young-scientists-forum-presenters-profiles-24-05.pdf

1 MBDownload

keynote-presenters-profiles-25-05-i.pdf

597.37 KbDownload
Start atSubject View AllNum.Add
 
Keynote Forum. Frontiers in Nano - materials/systems/interfaces Multifunctionality for Human Well - being : Organizers/ Chairs: Professors Bo Zhu (China ) & insung S.Choi (Korea), Dr.Donata Iandolo (UK), Dr.Maciej Cieplak (Poland)
09:15
Authors : Prof. Dr. Bert Muller
Affiliations : Biomaterials Science Center (BMC), University of Basel, Gewerbestrasse 14 4123 Allschwil Switzerlan bert.mueller@unibas.ch

Resume : Biomaterials nanoscience and nanotechnology development determinate Nanomedicine success because Nanomedicine, also termed nanotechnology-enabled medicine [1], is the science and technology of diagnosing, treating, and preventing diseases and traumatic injuries, of relieving pain, and of preserving and improving health using molecular tools and molecular knowledge of the human body according to the European Science Foundation. To clearly distinguish nanomedicine from established treatment forms, it can alternatively be defined as characterizing hard and soft tissues on the nanometer scale and tailoring nanostructured man-made materials for improving human health. The understanding of tissue organization down to its nanometer-size components and the development of interrelated tools to prevent, diagnose, and treat diseases are essential steps in current clinically applied science. The societal need for cost-effective improvements of patient health thereby motivates the research activities in nanomedicine and their scientific approaches [2]. These efforts are converged on targeting applications in orthopedics and dentistry; oral and musculoskeletal biology are both largely driven by mechanical forces, and the prosthesis materials in both applications require matching the unique nanostructure and material properties of the host tissues [3]. [1] B. Müller, Nanomedicine at a glance Book chapter 1 "in Nanoscience and Nanotechnology: Advances and Developments in Nano-sized Materials” (Ed. M.Van de Voorde) De Gruyter (2018) pp. 3-15 DOI:10.1117/12.2317867 [2] C. Netzer, P. Distel, U. Wolfram, H. Deyhle, G. F. Jost, S. Schären, J.Geurts Comparative analysis of bone structural parameters reveals subchondral cortical plate resorption and increased trabecular bone remodeling in human facet joint osteoarthritis International Journal of Molecular Sciences 19 (2018) 854 DOI:10.3390/ijms19030845 [3] C. Bikis, L. Degrugillier, P. Thalmann, G. Schulz, B. Müller, S. E.Hieber, D. F. Kalbermatten, S. Madduri Three-dimensional imaging and analysis of entire peripheral nerves after repair and reconstruction, Journal of Neuroscience Methods 295 (2018) 37-44 DOI:10.1016/j.jneumeth.2017.11.015

E.1.1
10:00
Authors : Insung S. Choi
Affiliations : Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Korea

Resume : Nature has developed a fascinating strategy of cryptobiosis for counteracting the stressful, and often lethal, environmental conditions. For example, certain bacteria sporulate to transform from a metabolically active, vegetative state to an ametabolic endospore state. The bacterial endospores, encased within tough biomolecular shells, withstand the extremes of harmful stressors, such as radiation, desiccation, and malnutrition, for extended periods of time and return to a vegetative state by breaking their protective shells apart when their environment becomes hospitable for living. Inspired by cryptobiosis found in nature, researchers have sought to chemically control and tailor the metabolic behaviors of non-spore-forming cells as well as enhancing their viability against adverse environmental conditions, by forming thin (< 100 nm), tough artificial shells [1-5]. These living ?cell-in-shell? structures, called artificial spores, enable chemical control of cell division, protection against physical and chemical stresses, and cell-surface functionalizability, armed with exogenous properties that are not innate to the cells but are introduced chemically. The field has further advanced to the stage of chemical sporulation and germination, where cytoprotective shells are formed on living cells and broken apart on demand. The (degradable) cell-in-shell hybrids are anticipated to find their applications in various biomedical and bionanotechnological areas, such as cytotherapeutics, high-throughput screening, sensors, and biocatalysis, as well as providing a versatile research platform for single-cell biology. In this sense, the artificial spore can be considered as a micrometric Iron Man: what is important is not the shell but the cell inside the shell.

E.1.2
14:00
Authors : Qichao Pan; Yaoqiong Zhang; Sihao Qian; Zhi Geng; Bo ZHU
Affiliations : College of Materials Science and Engineering, Shanghai University, 99 Shangda Road, BaoShan, Shanghai, 200444, China

Resume : Implanted electronics suffer from long-term stability of implanting, arisen from the immune response, nerve migration, bonding failure of electronic materials, etc. Toward these issues, we developed a series of bioinspired EDOT polymers integrating nonspecific-binding resistance, smart function and low-impedance. By defining structures for building blocks, the bioinspired conducting polymers could statically or dynamically electro-couple with cells at a high selectivity, strongly interact with targeted cells via a three-dimensional way, depress remarkably the immune response, or strongly bond the substrate to ensure the stable communication with cells. These bioinspired PEDOT materials demonstrated their potential as an intimate, stable and efficient electrical interface to cells.

E.1.3
14:45
Authors : Karsten Haupt,* Bernadette Tse Sum Bui, Paulina Medina Rangel
Affiliations : Sorbonne Universités, Université de Technologie de Compiègne UMR CNRS 7025 Enzyme and Cell engineering

Resume : Molecularly imprinted polymers (MIPs) are synthetic antibody mimics that specifically recognize molecular targets [1,2]. They are highly cross-linked polymers synthesized in the presence of the target molecule acting as a molecular template. This templating induces three-dimensional binding sites in the polymer that are complementary to the template in size, shape and chemical functionality. The synthetic antibody can recognize and bind its target with an affinity and selectivity similar to a biological antibody. We present here new approaches for the syn-thesis of MIP, by localized polymerization at the nanoscale. Photoinduced living radical polymerization using a dendritic multiiniferter was used to synthesize protein-size, soluble MIP nanogels with a homogeneous size distri-bution [3]. Their mean diameter is 17 nm and the average molecular weight 97 kDa, thus their size and density are very close to those of biological antibodies. The MIP nanogels show specific binding of their targets, small organic molecules or proteins, with a nanomolar affinity and a good selectivity. In addition, the direct coating of thin MIP films around fluorescent nanoparticles [4,5] by localized photopolymerization using the particles as individual internal light sources (Fig. 1), are described. The targets of these MIPs are cell surface glucosides, and peptide epitopes of cell surface proteins. The use of these functional nanomaterials as antibody-mimicking inhibitors of biological functions in biomedicine and cosmetics [6,7], for bioassays [8] as well as for cell and tissue imaging [5,9] by fluorescence microscopy will be demonstrated on fixated and living keratinocytes. References [1] R. Arshady, K. Mosbach, Makromol. Chem. 182 (1981) 687-692. [2] K. Haupt, et al., Top. Curr. Chem. 325 (2012) 1-28. [3] P. Çakir, et al. Adv. Mater. 25 (2013) 1048-1051. [4] S. Beyazit, et al. Angew. Chem. Int. Ed. 53 (2014) 8919-8923. [5] M. Panagiotopoulou et al. Angew. Chem. Int. Ed. 55 (2016) 8244-8248. [6] A. Cutivet et al. J. Am. Chem. Soc. 131 (2009) 14699-14702. [7] S. Nestora et al. Angew. Chem. Int. Ed. 55 (2016) 6252-6256. [8] J. Xu, K. Haupt, B. Tse Sum Bui, ACS Appl. Mater. Interfaces 9 (2017) 24476-24483. [9] M. Panagiotopoulou et al. Biosens. Bioelectron. 88 (2017) 85-93.

E.1.4
15:00
Authors : Yaopeng Zhang
Affiliations : State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, Shanghai, 201620, China

Resume : Native silk nanoribbons (SNRs) with a height of around 0.4 nm were exfoliated from natural silkworm silk (DS) by partially dissolving DS in eco-friendly alkaline solution. In the process of dissolving, the solvent does not destroy the nanofibril structure completely, but influenced the dimensions of SNRs. Transmission electron microscope, atomic force microscope, synchrotron radiation small angle X-ray scattering, and molecular dynamics simulations were applied to confirm the single molecular layer nanoribbon. It was found that SNRs containing single ?-sheet layer and amorphous structure are the basic building blocks of the DS with hierarchical structures. The novel partially dissolution method for fabricating SNRs may pave a new route for creating stronger and tougher silk based materials and further ensure the functionality and durability of materials in various applications, such as filtration, tissue engineering, biosensing and optics.

E.1.5
16:15
Authors : Jarno Salonen
Affiliations : Industrial Physics Laboratory, Department of Physics and Astronomy, University of Turku, FI-20014 Turku, Finland

Resume : Regenerative electroless etching (ReEtching) is a method of producing nanostructured semiconductors in which an oxidant (Ox1) is capable of initiating etching by injecting holes into the semiconductor valence band and a second oxidant (Ox2), which would be unreactive in the primary reaction, is used to regenerate Ox1. Thereby the extent and the rate of reaction are controlled by the amount and rate of Ox2 added in the process. This general strategy is demonstrated specifically for the production of highly luminescent, porous Si from the reaction of V2O5 in HF(aq) as Ox1 and H2O2(aq) as Ox2 with Si powder, chunks and wafers. Numerous applications of nanostructured silicon, e.g. consumer products, nanomedicine and biosensing, would benefit from economical production of porous powder on the kilogram to ton scale. However, that has been infeasible up until now because of the uncontrollable electroless etching processes. The new ReEtching method overcomes the problem and may be used in an industrial level production of mesoporous silicon. In addition to a feasible large-scale production of different kind of nanostructured Si structures, ReEtching can also be used to pre-anodized mesoporous silicon powder. The result is stunningly bright and persistent photoluminescence due to much smaller quantum-confined structures within the mesopores produced by ReEtching, also enabling biosensing, diagnostic and even theranostic use of ReEtched particles.

E.1.6
16:30
Authors : Arzum Erdem
Affiliations : Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova, 35100 Izmir, TURKEY * email: arzum.erdem@ege.edu.tr and arzume@hotmail.com

Resume : Electrochemical biosensors are coupling the inherent specifity of biorecognition reactions with the high sensitivity of physical transducers that hold a great potential for their implementation to the analysis of nucleic acids, proteins, toxins etc. related to clinical, environmental, or forensic investigations. The recent developments of nanoscale materials integrated biosensors based on graphenes, nanotubes, nanoparticles, dendrimers, nanorods, nanowires, and other nanomaterials have received the significant interest in different areas of biotechnology. The progress of advanced (bio)sensor technologies could impact considerably in the areas of genomics, proteomics, biomedical diagnostics, drug discovery as well as enviromental monitoring and food security. The nanomaterials integrated electrochemical biosensors developed for monitoring the biointeractions of nucleic acids, aptamer-protein, or drug-DNA etc. have been overviewed herein with their further applications. Acknowledgements. A.E acknowledges to the financial support from Turkish Scientific and Technological Research Council (TÜBİTAK) (Project No: 111T073 and 115Z099) as the Project Investigator, and she also would like to express her gratitude to the Turkish Academy of Sciences (TÜBA) as the Principal member for its partial support.

E.1.7
16:45
Authors : Maciej Cieplak,* Patrycja Lach, Piyush Sindhu Sharma, Marta Sosnowska, Krzysztof Noworyta, Francis D'Souza,* and Wlodzimierz Kutner*
Affiliations : Institute of Physical Chemistry, Polish Academy of Sciences (IPC PAS), Kasprzaka 44/52, 01-224 Warsaw, Poland; Department of Chemistry, University of North Texas, 1155 Union Circle, #305070, Denton, TX 76203-5017, USA; Faculty of Mathematics and Natural Sciences, School of Sciences, Cardinal Stefan Wyszynski University in Warsaw, Woycickiego 1/3, 01-815 Warsaw, Poland; e-mail: mcieplak@ichf.edu.pl

Resume : Molecularly imprinted polymers (MIPs) are excellent example of bio-mimicking recognition materials. They have found numerous applications in selective chemosensing. For electrochemical determination of electroinactive analytes, usually some external redox probe is added. It is assumed that binding of target analyte molecules by MIP molecular cavities causes MIP film swelling or shrinking. This behavior leads to changes in MIP film permittivity for the redox probe and thus changes in faradaic currents corresponding to reduction or oxidation of the probe (so called “gating effect”) in CV and DPV determinations. However, this mechanism seems to be inadequate for electrochemical sensors with conductive MIP film recognition units. It was proven that drop of the redox probe oxidation peak in DPV determination originates from changes in electrochemical properties of the MIP film. We can speculate that diffusion of a redox probe is a not crucial issue in terms of selective determination with the MIP film coated electrode. Therefore, a new specially designed monomer, vis., p-bis(2,2'-bithien-5-yl)methyl-ferrocene benzene was used for deposition of a self-reporting MIP film. This monomer acted as both a crosslinking monomer and an internal redox probe. The self-reporting MIP film modified electrodes were used for electrochemical determination of p-synephrine – a diet supplement that is suspected of causing serious cardiovascular diseases - in the absence of the external redox probe.

E.1.8
 
Special Poster Session "face-to-face" Keynote Presenter - Participants : Organizers/Chairs : Professors Arzum Erdem
17:45
Authors : Arzum Erdem
Affiliations : Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova, 35100 Izmir, TURKEY * email: arzum.erdem@ege.edu.tr and arzume@hotmail.com

Resume : Electrochemical biosensors are coupling the inherent specifity of biorecognition reactions with the high sensitivity of physical transducers that hold a great potential for their implementation to the analysis of nucleic acids, proteins, toxins etc. related to clinical, environmental, or forensic investigations. The recent developments of nanoscale materials integrated biosensors based on graphenes, nanotubes, nanoparticles, dendrimers, nanorods, nanowires, and other nanomaterials have received the significant interest in different areas of biotechnology. The progress of advanced (bio)sensor technologies could impact considerably in the areas of genomics, proteomics, biomedical diagnostics, drug discovery as well as enviromental monitoring and food security. The nanomaterials integrated electrochemical biosensors developed for monitoring the biointeractions of nucleic acids, aptamer-protein, or drug-DNA etc. have been overviewed herein with their further applications. Acknowledgements. A.E acknowledges to the financial support from Turkish Scientific and Technological Research Council (TÜB?TAK) (Project No: 111T073 and 115Z099) as the Project Investigator, and she also would like to express her gratitude to the Turkish Academy of Sciences (TÜBA) as the Principal member for its partial support.

E.P1.1
17:45
Authors : Qichao Pan; Yaoqiong Zhang; Sihao Qian; Zhi Geng; Bo ZHU
Affiliations : Shanghai Distinguished Experts, College of Materials Science and Engineering, Shanghai University, 99 Shangda Road, BaoShan, Shanghai, 200444, China, bozhu@shu.edu.cn

Resume : Advances of bioelectronics towards high-resolution communication and long-term implantation are reaching limitations of traditional materials such as metals and silicone. An ideal electronic material combining softness, biofunction and low impedance is desired to meet the required biocompatibility, and electrical trade-offs for interfacing with cells/tissues. Toward this aim, we developed a series of cell-membrane mimicking EDOT polymers, which combining biofunction with low impedance. These conducting polymers present a high selectivity of interaction to targeted cells and proteins. The selective interaction could be well controlled to be static, dynamic or even 3-dimensional by defining various structures for building blocks. All these materials demonstrated an intimate, stable and efficient electrical interface with targeted cells, which is ensured by integrating nonspecific-binding resistance, specific interaction and low-impedance into one PEDOT polymer. To ensure integrity of the bioinspired PEDOTs on electrodes in long term stimulation, we further developed a series of bioinspired PEDOTs with a very strong adhesion to electrode substrates. The electrodes of the bioinspired PEDOTs demonstrated their long-term stability to functionalize in aqueous buffer at low impedance.

E.P1.2
17:45
Authors : Professor Insung S.Choi
Affiliations : Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Korea

Resume : Nature has developed a fascinating strategy of cryptobiosis for counteracting the stressful, and often lethal, environmental conditions. For example, certain bacteria sporulate to transform from a metabolically active, vegetative state to an ametabolic endospore state. The bacterial endospores, encased within tough biomolecular shells, withstand the extremes of harmful stressors, such as radiation, desiccation, and malnutrition, for extended periods of time and return to a vegetative state by breaking their protective shells apart when their environment becomes hospitable for living. Inspired by cryptobiosis found in nature, researchers have sought to chemically control and tailor the metabolic behaviors of non-spore-forming cells as well as enhancing their viability against adverse environmental conditions, by forming thin (< 100 nm), tough artificial shells [1-5]. These living ?cell-in-shell? structures, called artificial spores, enable chemical control of cell division, protection against physical and chemical stresses, and cell-surface functionalizability, armed with exogenous properties that are not innate to the cells but are introduced chemically. The field has further advanced to the stage of chemical sporulation and germination, where cytoprotective shells are formed on living cells and broken apart on demand. The (degradable) cell-in-shell hybrids are anticipated to find their applications in various biomedical and bionanotechnological areas, such as cytotherapeutics, high-throughput screening, sensors, and biocatalysis, as well as providing a versatile research platform for single-cell biology. In this sense, the artificial spore can be considered as a micrometric Iron Man: what is important is not the shell but the cell inside the shell.

E.P1.3
17:45
Authors : Professor Yaopeng Zhang
Affiliations : State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, Shanghai, 201620, China

Resume : Native silk nanoribbons (SNRs) with a height of around 0.4 nm were exfoliated from natural silkworm silk (DS) by partially dissolving DS in eco-friendly alkaline solution. In the process of dissolving, the solvent does not destroy the nanofibril structure completely, but influenced the dimensions of SNRs. Transmission electron microscope, atomic force microscope, synchrotron radiation small angle X-ray scattering, and molecular dynamics simulations were applied to confirm the single molecular layer nanoribbon. It was found that SNRs containing single ?-sheet layer and amorphous structure are the basic building blocks of the DS with hierarchical structures. The novel partially dissolution method for fabricating SNRs may pave a new route for creating stronger and tougher silk based materials and further ensure the functionality and durability of materials in various applications, such as filtration, tissue engineering, biosensing and optics.

E.P1.4
17:45
Authors : Karsten Haupt,* Bernadette Tse Sum Bui, Paulina Medina Rangel
Affiliations : Sorbonne Universités, Université de Technologie de Compiègne UMR CNRS 7025 Enzyme and Cell engineering

Resume : Molecularly imprinted polymers (MIPs) are synthetic antibody mimics that specifically recognize molecular targets [1,2]. They are highly cross-linked polymers synthesized in the presence of the target molecule acting as a molecular template. This templating induces three-dimensional binding sites in the polymer that are complementary to the template in size, shape and chemical functionality. The synthetic antibody can recognize and bind its target with an affinity and selectivity similar to a biological antibody. We present here new approaches for the syn-thesis of MIP, by localized polymerization at the nanoscale. Photoinduced living radical polymerization using a dendritic multiiniferter was used to synthesize protein-size, soluble MIP nanogels with a homogeneous size distri-bution [3]. Their mean diameter is 17 nm and the average molecular weight 97 kDa, thus their size and density are very close to those of biological antibodies. The MIP nanogels show specific binding of their targets, small organic molecules or proteins, with a nanomolar affinity and a good selectivity. In addition, the direct coating of thin MIP films around fluorescent nanoparticles [4,5] by localized photopolymerization using the particles as individual internal light sources (Fig. 1), are described. The targets of these MIPs are cell surface glucosides, and peptide epitopes of cell surface proteins. The use of these functional nanomaterials as antibody-mimicking inhibitors of biological functions in biomedicine and cosmetics [6,7], for bioassays [8] as well as for cell and tissue imaging [5,9] by fluorescence microscopy will be demonstrated on fixated and living keratinocytes. References [1] R. Arshady, K. Mosbach, Makromol. Chem. 182 (1981) 687-692. [2] K. Haupt, et al., Top. Curr. Chem. 325 (2012) 1-28. [3] P. Çakir, et al. Adv. Mater. 25 (2013) 1048-1051. [4] S. Beyazit, et al. Angew. Chem. Int. Ed. 53 (2014) 8919-8923. [5] M. Panagiotopoulou et al. Angew. Chem. Int. Ed. 55 (2016) 8244-8248. [6] A. Cutivet et al. J. Am. Chem. Soc. 131 (2009) 14699-14702. [7] S. Nestora et al. Angew. Chem. Int. Ed. 55 (2016) 6252-6256. [8] J. Xu, K. Haupt, B. Tse Sum Bui, ACS Appl. Mater. Interfaces 9 (2017) 24476-24483. [9] M. Panagiotopoulou et al. Biosens. Bioelectron. 88 (2017) 85-93.

E.P1.5
Start atSubject View AllNum.Add
 
Young Scientists Forum. Frontiers in Nano - materials/systems/interfaces Multifunctionality for Human Well – being : Organizers/Chairs: Katharina Brassat (Germany), Joana M.Vasconcelas & Federico Zen (Ireland). Supervisors: Maciej Cieplak (Poland) & Donata Iandolo (UK)
09:00
Authors : Bernard L. Feringa
Affiliations : Synthetic Organic Chemistry ? Stratingh Institute for Chemistry, Faculty of Science and Engineering, University of Groningen, Groningen, The Netherlands, b.l.feringa@rug.nl

Resume : The design and construction of dynamic functional molecular systems, which mimic some of the properties of living systems, poses a huge contemporary challenge. Recent developments in supramolecular self -assembly, molecular switches, motors and machines, and chemical reaction networks, offer an excellent basis for integrating dynamic properties in molecular systems. In this perspective, we discuss different approaches towards dynamic functional molecular systems covering areas such as translated motion, dissipative self-assembly, self-regulation and biohybrid systems. The selected examples illustrate the level of control and complexity that can be achieved at present in this rapidly growing and exciting field of research.

E.YSF.1
09:50
Authors : K. Brassat
Affiliations : Nanostructuring, Nanoanalysis and Photonic Materials group, Dept. of Physics, Paderborn University, Germany

Resume : Biological and bioinspired units such as protein micelles, lipid vesicles or peptide macromolecules offer manifold functionalities at high specificity. Research in life and materials sciences develops methods to make these functional bio units accessible for next generation devices for e.g. sensing, molecular electronics or bio photonics. Most bio units form in liquid suspensions. This is a suitable environment for e.g. drug delivery systems, however is a drawback for the integration of bio units into electronic devices. Facing this challenge, here we present an approach for the site-selective deposition of single functional bio units, i.e. protein micelles and DNA origamis, into ordered arrays on large areas of solid surfaces. In particular, we use nanosphere lithography as a bottom-up approach for the nanopatterning of different material surfaces. We create nanohole arrays, i.e. ordered cylindrical holes in thin films, exhibiting a surface topography along with a local material contrast. We use these nanoholes as templates for the site-selective deposition of casein micelles. We take advantage of the templates chemical contrast. We locally perform an enzyme mediated autodeposition and create nanostructured biocoatings. In another example, we site-selectively adsorb DNA origamis inside the nanoholes. These origamis are for instance suitable transporters for quantum dots enabling bioinspired next generation nanoelectronics.

E.YSF.2
10:10
Authors : Alice C. Taylor, Citalali Gonzalez, Patrizia Ferretti, Stoffel Janssens, Richard B. Jackman, Eliot Fried
Affiliations : Okinawa Institiute of Science and Technology 1919-1 Tancha, Onna-son, Kunigami-gun Okinawa, Japan 904-0495 London Centre for Nanotechnology Department of Electronic and Electrical Engineering University College London 17-19 Gordon Street, London, WC1H 0AH, UK

Resume : The recent rise in life expectancy has led an increase in the number of cases of neurological diseases such as Alzheimer?s, Parkinson?s and macular degeneration. Traditional therapeutic approaches are ineffective as regeneration is limited in the Central Nervous System (CNS). Neural prosthetics and stem cell therapy present exciting solutions for enabling the function of the brain to be restored. Implanted materials for neuronal prosthetics must have outstanding electrical properties whilst being inert and biocompatible. Neural stem cells (NSCs) have great potential for inducing repair in damaged areas of the central nervous system (CNS). NSCs have the ability to self-renew, but also are able to differentiate into the main cells in the CNS: neurons, oligodendrocytes and astrocytes. Understanding the differentiation into these cell lineages is critical for regenerative therapy treatment of such for mentioned neurological diseases as detailed knowledge of how these specific cells are affected by disease is vital (1). In order to utilise the potential of stem cells in the field of regenerative medicine, it is essential that we are able to isolate the cells from their natural setting, propagate the cells in culture, and introduce the cells to a foreign environment (2). Given the outstanding bioactivity shown by nanodiamonds (NDs) towards neurons (3), the proliferation and differentiation of human NSCs (hNSCs) and adipose derived stem cells (ADSCs) and their relationship with diamond has been investigated. Here, the biocompatibility of diamond upon boron inclusion and nanostructuring has been investigated, as well as the interaction of hNSCs and ADSCs with functionalised NDs. Oxygen-NDs significantly favouring the proliferation and adhesion of hNSC over Hydrogen-NDs and the tissue culture polystyrene (TCPS) control. Contact angle and protein adsorption investigations suggesting why O-NDs are highly suited for hNSC growth. Also, ND surfaces with O and H functionalisation are shown to influence the differentiation of hNSCs in varying ways, with hNSCs fate being investigated via both inductive and spontaneous differentiation assays. 1. Lindvall O, Kokaia Z. Stem cells for the treatment of neurological disorders. Nature. Nature Publishing Group; 2006 Jun 29;441(7097):1094?6. 2. Scadden DT. The stem-cell niche as an entity of action. Nature. 2006 Jun 29;441(7097):1075?9. 3. Thalhammer A, Edgington RJ, Cingolani LA, Schoepfer R, Jackman RB. The use of nanodiamond monolayer coatings to promote the formation of functional neuronal networks. Biomaterials. 2010 Mar;31(8):2097?104.

E.YSF.3
10:30 Coffee Break    
11:00
Authors : Donata Iandolo,1 Charalampos Pitsalidis,1 Francesca Santoro,2,3 Bianxiao Cui,3 Roisin M. Owens.1
Affiliations : 1Department of Chemical Engineering and Biotechnology, University of Cambridge (UK), 2Center for Advanced Biomaterials for Healthcare, IIT (Italy), 3Department of Chemistry, Stanford University (US).

Resume : One of the latest trends in the fields of tissue engineering as well as oncological research is the development of in vitro 3D systems mimicking tissues or organs in vitro. Indeed, there is an increasing demand for biomimetic in vitro models recapitulating the tridimensional structure and microenvironment experienced by cells in vivo. In addition to chemical and mechanical cues, some tissues are known to be regulated by endogenous bioelectrical cues. One such tissue is bone. It has also been demonstrated to exhibit piezoelectric properties in vivo. Electroactive scaffolds, based on conducting polymers, are promising candidates to address bone piezoelectricity and to enhance osteogenesis in vitro as well as in vivo. Herein, we describe the development of a biomimetic 3D model of bone tissue based on the conducting polymer PEDOT:PSS and human adipose derived stem cells. 3D electroactive porous scaffolds were produced using the ice-templating technique. Different PEDOT:PSS to Collagen ratios) were explored. Pores sizes, mechanical and impedance properties were measured as a function of scaffolds composition. Osteogenic differentiation studies were run and the different compositions were assessed for their impact on stem cells fate. SEM/FIB was used to investigate the interaction between materials and cells, highlighting an intimate contact of the cells lining the pores walls. The results obtained so far underline the usefulness of the developed porous conductive scaffolds as in vitro platforms to study hADSCs for bone tissue engineering.

E.YSF.4
11:20
Authors : Nanasaheb D. Thorat1, Joanna Bauere2 and Syed A.M. Tofail1
Affiliations : 1. Material and Surface Science Institute, Bernal Institute, University of Limerick, Limerick, V94 T9PX, Ireland 2. Department of Biomedical Engineering, Politechnika Wroclawska, Wroclaw, Poland

Resume : Cancer cells can become resistant to chemotherapeutic drugs and pose a challenging impediment for oncologists in providing effective chemotherapy treatment. Nanomedicine may allow overcoming chemoresistance and is the focus of our investigation. Here we show the validity of nanomedicine approach for targeted chemotherapeutic cargo delivery to overcome chemoresistance in cancer cells both in vitro and in vivo. For this, we functionalise ~100 nm long porous silica nanoparticles (~20 nm diameter ordered pore structure) by conjugating anticancer drug, cytochrome c enzyme and dual-function anticancer aptamer AS1411 in single supra-assembled nanocargos. The supra-assembly on the porous silica nanostructure allows for a high loading of catalytic enzyme cytochrome c, anticancer drug and aptamer. The silica supra-assembly is characterized by transmission electron microscopy (TEM) and BET analysis. Conjugation of cargoes has been monitored at each step by UV-Vis and Fluorescence spectroscopy. Finally, the constructed supra-assembled nanocarrier tested in vitro and in vivo. A pH-responsive, intracellular theranostic cargo delivery has been achieved and the triple action of the nanocargo made an efficient killing of drug resistance colon cancer cells in vitro by supressing the P-glycoprotein (P-gp) level. The nanocargos displayed triplex therapy effects on the drug resistance cancer cells both in vitro and in vivo

E.YSF.5
11:35
Authors : Indranath Chakraborty,?? Neus Feliu,?§ Sathi Roy,?? Kenneth Dawson,? and Wolfgang J. Parak*???
Affiliations : ? Fachbereich Physik, Philipps Universität Marburg, 35037 Marburg, Germany ? Fachbereich Physik und Chemie, and Center for Hybrid Nanostructure (CHyN), Universität Hamburg, 22761 Hamburg, Germany § Department of Laboratory Medicine (LABMED), Karolinska Institutet, 171 77 Stockholm, Sweden ? Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Dublin 4, Ireland ? CIC Biomagune, 20014 San Sebastian, Spain

Resume : Tuning nanoparticles surfaces is very important to introduce new properties to the system such as solubility, self-assembly, sensing, biocompatibility, etc.1, 2 This talk will explain the growth of silver nanoparticles in aqueous solution, without the presence of typical surfactant molecules, but under the presence of different proteins.3 The shape of the resulting silver nanoparticles could be tuned by the selection of the types of proteins. The number of accessible lysine groups was found to be mainly responsible for the anisotropy in nanoparticle formation. Viability measurements of cells exposed to protein capped spherical or prism-shaped NPs did not reveal differences between both geometries. Thus, in the case of protein protected Ag NPs, no shape-induced toxicity was found under the investigated exposure conditions.

E.YSF.6
11:50
Authors : Abubakre O. K., Medupin R. O., Abdulkareem A. S., Muriana R. A.
Affiliations : Mechanical Engineering Department, Federal University of Technology, Minna, Nigeria Chemical Engineering Department, Federal University of Technology, Minna, Nigeria Nanotechnology Group, Centre for Genetic Engineering and Biotechnology, Federal University of Technology, Minna, Nigeria

Resume : The desire to restore the quality of life to transtibial amputees in Nigeria has been on the front burner in recent years. In this study, a home-grown nanocomposite (NC) material (multi-walled carbon nanotube reinforced natural rubber) and multiflex dynamic response 2 foot (a common foreign foot prosthetics in Nigeria) were investigated with a view to comparing their water absorption capacity, thermal stability, wear resistance and morphological properties. The inherent challenge of ensuring uniform distribution of multi-walled carbon nanotube (MWCNT) in the host matrix was addressed by the use of sodium dodecylbenzene sulfonate (C18H29NaO3S). The CNT was synthesised via catalytic chemical vapour deposition (CCVD) technique and the NC was produced using an electrically heated hydraulic press. While the initial decomposition temperatures (Tonset) of the materials show that the newly developed NC with 260.01 oC is more thermally stable than M. DR2 foot with the temperature of 238.17 oC, incorporation of MWCNTs into the unfilled NR matrix shows a significant change in Tonset. MWCNT loading was found to influence the moisture content of the reinforced matrix by about 7% with the NC being 35% more thermally stable than M. DR2 foot. SEM/EDS micrographs indicated complete embedment of MWCNTs in NR matrix thereby making it more suitable than M. DR2 foot which was inundated with cavities. While it takes both NR/MWCNT and DR2 foot 120 days to attain saturation point in water, the former is 93% more dimensionally stable than the latter and also demonstrated better resistance to wear than the latter. It can, therefore, be concluded from the foregoing that the home-grown material is to be preferred to its foreign counterpart for anthropomorphic prosthetic foot application. Keywords ? Natural rubber, NR/MWNT, TGA/DTG, SEM/EDS, Water Absorption, DR2 foot, Wear Resistance

E.YSF.7
13:00 Lunch Break    
14:00
Authors : Zhi Geng, Bo Zhu
Affiliations : Zhi Geng, State Key Lab for Modification of Chemical Fibers and Polymer Materials& College of Materials Science and Engineering, Donghua University; Bo Zhu, , College of Materials Science and Engineering, Shanghai University

Resume : Poly(3,4-ethylenedioxythiophene) (PEDOT) materials was considered as star materials for fabricating bioelectronic devices 1-4 due to its excellent electrical conductivity and electrochemical stability5,6. Decoration with functional groups and introducing nanostructures would enhance the electric and biological properties of PEDOT materials, which could expand the potential applications in the fields of bioelectronics. Therefore, it was important to design simple but controllable nano-assembling methods for functionalized PEDOT materials. Compared to traditional template assembling, template-free method had the beneficial of easy fabrication in large scale and integrated preservation of achieved nanostructures, which would make it possible for fabricating nano electronic devices in wafer-scale. Several methods had been reported to realize nano assembling PEDOT materials, however, few of them could realize fabricating different types of nano-morphologies for various functionalized EDOT monomers with one simple method. Herein, we proposed template-free method for fabricating various functionalized PEDOT thin films with nanodot and nanotube morphologies via electrochemical depositing. The morphology parameters could be tuned including diameter, length and density. Glancing incident wide angle XRD was measured to testify the difference of crystal orientation for the achieved nanodot and nanotube thin films. Meanwhile, possible formation reason and process of nanodot and nanotube morphologies were proposed. Besides, simulation tests were carried out and verified the beneficial of the PEDOT and functionalized PEDOT thin films with nanotube morphology using in bioelectronic devices. We hoped the achieved template-free assembling method could be expanded to other kinds of bio-functionalized PEDOT materials and designed PEDOT thin films with proper bio-functional groups and 3D nanomorphologies based on the requirements in practical application of bioelectronic devices. Reference: 1. Khodagholy, D.; Rivnay, J.; Sessolo, M.; Gurfinkel, M.; Leleux, P.; Jimison, L. H.; Stavrinidou, E.; Herve, T.; Sanaur, S.; Owens, R. M.; Malliaras, G. G. Nat Commun 2013, 4, 2133. 2. Rivnay, J.; Inal, S.; Collins, B. A.; Sessolo, M.; Stavrinidou, E.; Strakosas, X.; Tassone, C.; Delongchamp, D. M.; Malliaras, G. G. Nat. Commun. 2016, 7, 11287. 3. Williamson, A.; Rivnay, J.; Kergoat, L.; Jonsson, A.; Inal, S.; Uguz, I.; Ferro, M.; Ivanov, A.; Sjostrom, T. A.; Simon, D. T.; Berggren, M.; Malliaras, G. G.; Bernard, C. Advanced materials 2015, 27, 3138. 4. Williamson, A.; Ferro, M.; Leleux, P.; Ismailova, E.; Kaszas, A.; Doublet, T.; Quilichini, P.; Rivnay, J.; Rozsa, B.; Katona, G.; Bernard, C.; Malliaras, G. G. Advanced materials 2015. 5. Sotzing, G. A.; Briglin, S. M.; Grubbs, R. H.; Lewis, N. S. Analytical chemistry 2000, 72, 3181. 6. Jalili, R.; Razal, J. M.; Innis, P. C.; Wallace, G. G. Advanced Functional Materials 2011, 21, 3363.

E.YSF.8
14:00
Authors : Qianqian Niu, Yaopeng Zhang
Affiliations : State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, Shanghai, 201620, China

Resume : Silkworm silk and spider silk with outstanding properties have unique hierarchical structures at mesoscale. As the basic building block of the hierarchical structure, silk nanofibrils (SNF) is the key unit for the formation of high performance silk based materials. Conventional methods to prepare SNFs have some limitations, such as nanofiber aggregation/inadequate dissociation, low yield, toxic solvent, imperfect building model of silk hierarchical structure etc. To fabricate stable SNFs suspension efficiently, a novel solution system was used to prepare SNFs. Transmission electron microscope, atomic force microscope, and synchrotron radiation small angle X-ray scattering were applied to confirm the size of SNFs accurately. Moreover, a SNF ultra thin film with good flexibility, high transmittance, biocompatibility and biodegradability were fabricated via vacuum filtration from the SNFs suspension. The film may have potential application for biosensing devices, optics, photonics or tissue engineering.

E.YSF.9
14:00
Authors : Christopher Jay T. Robidillo, Jonathan G.C. Veinot
Affiliations : Department of Chemistry University of Alberta

Resume : Diseases typically result from the accumulation of substances that disrupt the normal operation of cells. The introduction of enzymes, catalytic protein molecules essential for normal biological function, which act on such substances into affected tissues provides an attractive alternative for curing such diseases. Silicon nanocrystals, owing to their limited toxicity and photodynamics, offer a potentially safer and more efficient bioimaging platform compared to status quo organic dyes. Thus, a hybrid material consisting of enzymes that have been interfaced with silicon nanocrystals could offer simultaneous imaging and therapy. This study reports, for the first time, methods for the preparation of enzyme-conjugated silicon nanocrystals from native enzymes and acid or alkene-terminated silicon nanocrystals through the amide coupling and thiol-ene reactions, respectively. Model enzymes, glucose oxidase and lactase were successfully immobilized on silicon nanocrystals as confirmed by Fourier Transform Infrared Spectroscopy and X-ray Photoelectron Spectroscopy. Moreover, single reaction and cascade kinetic assays confirm that the conjugated enzymes retain their catalytic activity. The hybrids manifested either excellent solubility or good dispersibility in buffer, and were photostable, exhibiting bright orange photoluminescence even after more than a month of dispersion in an aqueous medium. The methods reported herein are general and can be used for the preparation of bioinorganic silicon-based hybrids that can be employed in personalized medicine for targeting and potentially treating diseases like cancer and other metabolic disorders.

E.YSF.10
14:00
Authors : Paulina X. Medina-Rangel,* Bernadette Tse Sum Bui, Karsten Haupt
Affiliations : Sorbonne Universités, Université de Technologie de Compiègne, CNRS UMR 7025 Enzyme and Cell Engineering

Resume : Advanced affinity tools for cell imaging are of particular interest as they can detect, localize and quantify molecular targets. Aberrant gly-cosylation sites and deregulated expression of growth factor receptors are promising bi-omarkers of many human diseases, most no-tably cancer. However, targeting these bi-omarkers is often challenging due to a lack of receptor molecules. Molecularly imprinted pol-ymers (MIPs) are tailor-made synthetic recep-tors (antibody mimics), able to specifically rec-ognize target molecules. They are synthesized by co-polymerizing functional and cross-linking monomers in the presence of a molecular template, resulting in the formation of binding sites with affinities and specificities compara-ble to those of natural antibodies [1]. Herein, we demonstrate biotargeting and bi-oimaging with fluorescently labeled MIPs on two different cancer biomarkers: hyaluronan and a growth factor receptor protein. MIPs were synthesized using a solid-phase synthe-sis approach in which an epitope of the bi-omarkers was immobilized on glass beads (as solid support) via click chemistry. This configu-ration allows an oriented immobilization of the template upon which thermoresponsive MIP nanoparticles were synthesized. The binding sites of the resulting MIPs all have the same orientation, thus MIPs synthesized by the sol-id-phase approach can be considered analo-gous to monoclonal antibodies [2-3]. Hyaluronan imaging was achieved by applying rhodamine-doped MIPs specific for glucuronic acid (an epitope of hyaluronan) on fixed hu-man keratinocytes [4]. Hyaluronan is com-posed of alternating units of D-glucuronic acid (GlcA) and N-acetyl-D-glucosamine. Thus, azide functionalized-glucuronic acid was im-mobilized on glass beads bearing alkyne groups. MIP-GlcA (70 nm) as water soluble particles were found to bind selectively extra-cellular, intracellular and nuclear hyaluronan, as imaged by epifluorescence and confocal microscopies. The specificity of bind-ing was verified with a non-imprinted control polymer and by comparing the staining with a hyaluronan binding protein. For bioimaging the growth factor receptor membrane protein, a short peptide (terminal alkyne functionalized) was selected as epitope for immobilization on azide-modified glass beads. The MIP nanoparticles (50 nm) specifi-cally recognized both the template peptide and the whole protein. Cell imaging studies with fluorescent dye-doped MIPs were per-formed. RFERENCES: [1] K. Haupt, A. V. Linares, M. Bompart and B. Tse Sum Bui, Top. Curr. Chem. 325 (2012), 1-28 [2] S. Ambrosini, S. Beyazit, K. Haupt and B. Tse Sum Bui, Chem. Commun. 49 (2013), 6746-6748 [3] J. Xu, P.X. Medina-Rangel, K. Haupt and B. Tse Sum Bui, Methods Enzymol. 590 (2017), 115-141 [4] M. Panagiotopoulou, S. Kunath, P. X. Medina-Rangel, K. Haupt and B. Tse Sum Bui, Biosens. Bioelectron. 88 (2017), 85-93

E.YSF.11
14:00
Authors : Hiromasa Murata, Takashi Suemasu, Kaoru Toko
Affiliations : University of Tsukuba

Resume : Multilayer graphene (MLG) has been actively investigated because of its high electrical and thermal conductivities. Because graphene has a unique two-dimensional structure, its characteristics are anisotropic. Therefore, large-grained highly oriented MLG on insulators is highly desirable. Metal-induced layer exchange (MILE) is a promising technique allowing for large-grained, highly-oriented Ge and Si on glass [1]. In this study, we applied MILE to amorphous carbon (a-C) and fabricated high-quality MLG at as low as 500 °C. Ni and a-C thin films (each 50 nm thick) were sequentially prepared on glass using magnetron sputtering. Samples were annealed at 500 °C for 50 h. The Ni layers were then etched away. The Raman spectra of back side of the samples have sharp D, G, and 2D peaks corresponding to MLG, indicating the layer exchange between the C and Ni layers [2,3]. The cross-sectional TEM analyses showed that the layers of a-C and Ni were exchanged and {002} oriented MLG formed on glass substrate. The grain size was approximately several hundred nm. After the removal of the Ni layers, the MLG covered the entire substrate. The electrical conductivity was approximately 400 Scm< sup>-1< /sup>. The uniformity and electrical conductivity are the highest level among the MLG directly formed on glass at low temperature. [1] Toko et al., APL. 104, 022106 (2014). [2] Murata et al., APL. 110, 033108 (2017). [3] Murata et al., APL. 111, 243104 (2017). (Highlighted in Nature INDEX.)

E.YSF.12
15:30 Coffee Break    
16:00
Authors : Vidya N Chamundeswari*, Say Chye Joachim Loo*#
Affiliations : *School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 #Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551

Resume : Electrospinning has emerged as a versatile, cost effective and reliable technique for fabrication of micro/nano fibers and particles with a strict regulation of solution parameters. However, there is minimal evidence till date of any successful dual structured particle on fiber “Sandwich”drug delivery system obtained from electrospinning that could provide controlled release of two or more different drugs. In this study we have successfully fabricated a dual structured electrospun fibrous - micro particle system through electrospinning for delivery of a hydrophilic and a hydrophobic drug simultaneously. A blend of two FDA approved biodegradable polymers was used as the electrospinning solution. Preliminary work focused on optimization of the processing parameters to arrive at 5-8μm porous particles that were electro-sprayed on a fibrous mesh network of PLGA-PCL fibres. The particles were further protected by another fibrous mesh on the top to prevent its loss during post fabrication, thereby the entire system resembling a sandwich. Characterization of the scaffold was carried out using Scanning Electron Microscopy, Rheometer and Fourier transform Infrared Spectroscopy. The hydrophobic Dexamethasone (Dex) was loaded into the fibers while Ascorbic acid (AA) was the hydrophilic biomolecule loaded into the particles. The release profiles of these drugs were quantified with the help of High Performance Liquid Chromatography (HPLC) which enabled the detection of Dex at 246nm and AA at 262 nm respectively. These scaffolds were used to study the differentiation capabilities of mesenchymal stem cells (MSCs) into osteo and chondro lineage. Future studies are directed towards understanding the influence of the carrier morphology and the synergistic effect of multiple biomolecules will be explored in depth for musculo-skeletal tissue engineering.

E.YSF.13
17:00
Authors : S. Franchi1, Y. Kosto2, A. Zanut3, I. Khalakhan2, Y. Yakovlev2, G. Valenti3, K. C. Prince1, F. Paulocci3, N. Tsud2, V. Matolin2
Affiliations : 1 Elettra-Sincrotrone Trieste S.C.p.A., in Area Science Park, Strada Statale 14, km 163.5, Basovizza (Trieste), 34149, Italy 2 Charles University, Faculty of Mathematics and Physics, Department of Plasma and Surface Science, V Hole?ovickach 2, 18000, Prague, Czech Republic 3 University of Bologna, Department of Chemistry ??G. Ciamician??, Via Selmi 2, 40126 Bologna, Italy. stefano.franchi79@gmail.com

Resume : Hydrogen peroxide (H2O2) is a strong oxidizing compound. It is involved in signalling and defence responses in biological systems, where enzymes (oxidases) reduce it to H2O through different pathways. In food and clinical analysis, concentrations of organic molecules are quantified by measuring the H2O2 produced by the coupled activity of oxidases. Although many methods are available for H2O2 detection, they are not suitable for industrial application. Electrochemical techniques, such as voltammetry, are advantageous for low level H2O2 detection in industrial applications because they are simple, fast and sensitive. Since commonly used electrodes are subject to non-linearity in their response, poisoning, and interference with collateral reactions when complex matrices are involved, we here propose an alternative sensing system. Cerium oxide (CeO2) nanoparticles show oxidase-like activity due to their capacity to change electronic configuration and exhibit oxygen vacancies, or defects, in the lattice structure. We conducted an electrochemical, morphological and spectroscopic study aimed at investigating the performance of polycrystalline CeO2 thin layers on Glassy Carbon electrodes as redox sensors, detecting micromolar concentrations of H2O2 in Phosphate Buffer Solution. Surface characterization was performed by SEM and AFM imaging before and after the electrochemical measurements, while system stability was investigated by Synchrotron Radiation Photoelectron Spectroscopy.

E.YSF.14
 
Special Poster Session of Young Scientist Forum with Evening Party : Organizers/Chairs: Katharina Brassat (Germany), Joana M.Vasconcelas & Federico Zen (Ireland). Supervisors: Maciej Cieplak (Poland) & Donata Iandolo (UK)
17:30
Authors : S. Franchi1, Y. Kosto2, A. Zanut3, I. Khalakhan2, Y. Yakovlev2, G. Valenti3, K. C. Prince1, F. Paulocci3, N. Tsud2, V. Matolin2
Affiliations : 1 Elettra-Sincrotrone Trieste S.C.p.A., in Area Science Park, Strada Statale 14, km 163.5, Basovizza (Trieste), 34149, Italy 2 Charles University, Faculty of Mathematics and Physics, Department of Plasma and Surface Science, V Holešovickach 2, 18000, Prague, Czech Republic 3 University of Bologna, Department of Chemistry ‘‘G. Ciamician’’, Via Selmi 2, 40126 Bologna, Italy.

Resume : Hydrogen peroxide (H2O2) is a strong oxidizing compound. It is involved in signalling and defence responses in biological systems, where enzymes (oxidases) reduce it to H2O through different pathways. In food and clinical analysis, concentrations of organic molecules are quantified by measuring the H2O2 produced by the coupled activity of oxidases. Although many methods are available for H2O2 detection, they are not suitable for industrial application. Electrochemical techniques, such as voltammetry, are advantageous for low level H2O2 detection in industrial applications because they are simple, fast and sensitive. Since commonly used electrodes are subject to non-linearity in their response, poisoning, and interference with collateral reactions when complex matrices are involved, we here propose an alternative sensing system. Cerium oxide (CeO2) nanoparticles show oxidase-like activity due to their capacity to change electronic configuration and exhibit oxygen vacancies, or defects, in the lattice structure. We conducted an electrochemical, morphological and spectroscopic study aimed at investigating the performance of polycrystalline CeO2 thin layers on Glassy Carbon electrodes as redox sensors, detecting micromolar concentrations of H2O2 in Phosphate Buffer Solution. Surface characterization was performed by SEM and AFM imaging before and after the electrochemical measurements, while system stability was investigated by Synchrotron Radiation Photoelectron Spectroscopy.

E.PYSF.1
17:30
Authors : MSc Malgorzata Murawska, PhD Wiktor Szymanski
Affiliations : Centre for Systems Chemistry, Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands, University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093 Warsaw, Poland e-mail: w.c.szymanski@rug.nl , w.szymanski@umcg.nl

Resume : (project) Experimental methodic characterization and obtained results of investigations are presented and discussed.

E.PYSF.2
17:30
Authors : Bozena Sikora, Izabela Kamińska1, Krzysztof Fronc1, Przemysław Kowalik1, Jakub Mikulski1, Maciej Szewczyk2,3, Anna Konopka4, Roman Minikayev1, Tomasz Wojciechowski1, Andrzej Sienkiewicz5,6, Mariusz Łapiński7, Sławomir Kret1, Piotr Stępień3,4,8, Grzegorz Wilczyński4, Wojciech Paszkowicz1, Danek Elbaum1 sikorab@ifpan.edu.pl
Affiliations : 1 Institute of Physics, Polish Academy of Sciences, al Lotników 32/46, 02-668 Warsaw, Poland. 2 Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw.3 Institute of Biochemistry and Biophysics PAN, Pawińskiego 5a, 02-106 Warsaw.4 Nencki Institute of Experimental Biology PAN, Pasteura 3, 02-093 Warsaw.5 Laboratory of Physics of Complex Matter, EPFL, Station 3, CH-1015 Lausanne, witzerland.6ADSresonaces, CH-1028 Préverenges, Switzerland.7 Institute of Optoelectronics, Military University of Technology, Gen. S. Kaliskiego 2, 00-908 Warsaw.8 Centre of New Technologies, Ochota, University of Warsaw, S. Banacha 2c, 02-097, Warsaw.

Resume : Nanoconstructs based on NaYF4 doped rare earth ions and Fe3O4 nanoparticles (SPIONs) co-capsulated in SiO2 were designed, synthesized, optimized and characterized. These nanoconstructs reveal simultaneously unique magnetic and photo-luminescent properties. In particular, nanoconstructs combine the capability of up-conversion of near infrared (NIR) to visible (VIS) or ultraviolet (UV) light with strong paramagnetic or superparamagnetic properties. The capability of up-conversion has the potential for clinical applications of imaging of diseased tissues and for in situ generation of reactive oxygen species (ROS). The latter process occurs via energy transfer from VIS-light emitting NaYF4 nanoparticles to photosensitizer molecules, which are attached to their surface. As such, the functionalized up-converting β-NaYF4 nanoparticles can be used for both photodynamic diagnosis (PDD) and therapy (PDT). Additionally, paramagnetic and superparamagnetic properties of the nanoconstructs offer numerous advantageous functionalities, including: nanoparticles tracking with the external magnetic field, enhanced contrast in magnetic resonance imaging (MRI), as well as diseased tissue eradication via local heating with alternating magnetic field (hyperthermia). β-NaYF4 nanoparticles with sizes < 20 nm, having relatively high efficiency of up-conversion luminescence (UCL), were synthesized by co-precipitation. The whole palette of UCL emission bands, resulting from the presence of various rare earth ions, was obtained. Moreover, under NIR light stimulation, the UCL of these nanoconstructs could excite molecules of a selected photosensitizer, Rose Bengal, towards the efficient generation of ROS.

E.PYSF.3
17:30
Authors : Christopher Jay T. Robidillo Jonathan G. C. Veinot
Affiliations : Department of Chemistry University of Alberta

Resume : Diseases typically result from the accumulation of substances that disrupt the normal operation of cells. The introduction of enzymes, catalytic protein molecules essential for normal biological function, which act on such substances into affected tissues provides an attractive alternative for curing such diseases. Silicon nanocrystals, owing to their limited toxicity and photodynamics, offer a potentially safer and more efficient bioimaging platform compared to status quo organic dyes. Thus, a hybrid material consisting of enzymes that have been interfaced with silicon nanocrystals could offer simultaneous imaging and therapy. This study reports, for the first time, methods for the preparation of enzyme-conjugated silicon nanocrystals from native enzymes and acid or alkene-terminated silicon nanocrystals through the amide coupling and thiol-ene reactions, respectively. Model enzymes, glucose oxidase and lactase were successfully immobilized on silicon nanocrystals as confirmed by Fourier Transform Infrared Spectroscopy and X-ray Photoelectron Spectroscopy. Moreover, single reaction and cascade kinetic assays confirm that the conjugated enzymes retain their catalytic activity. The hybrids manifested either excellent solubility or good dispersibility in buffer, and were photostable, exhibiting bright orange photoluminescence even after more than a month of dispersion in an aqueous medium. The methods reported herein are general and can be used for the preparation of bioinorganic silicon-based hybrids that can be employed in personalized medicine for targeting and potentially treating diseases like cancer and other metabolic disorders.

E.PYSF.4
17:30
Authors : Won-Yong Jin, Hock Beng Lee, Ovhal Manoj Mayaji, Jae-Wook Kang*
Affiliations : Graduate School of Flexible and Printable Electronics, Chonbuk National University

Resume : Textile based optoelectronic devices have been widely investigated for wearable device application because of its wearability, lightweight, ease of processing, and low-cost production. Particularly, textile based organic photovoltaics (OPVs) have shown promising results. However, the photovoltaic performance of textile OPV is hindered by the non-uniform coating of conductive fibers and high sheet resistance of electrode. In this work, highly flexible, transparent hybrid conducting electrodes (TCE) were fabricated via embedding silver sub-electrodes/conductive polymers into an UV-curable polymer. The photovoltaic performance with active layer consisting of PTB7-Th:PC71BM shows a relatively high power conversion efficiency (PCE) of ~ 5% with an active area of 0.3 cm2. To prepare functional textile OPVs, multiple devices were weaved and integrated into the fiber thread with various aperture area. An output voltage of > 7 V was achieved by connecting seven devices in series. Additionally, flexible supercapacitor (SCs) were integrated with solar cells as textile based power pack. The SCs were fabricated based on PEDOT:PSS electrodes sandwiched between gel electrolyte in symmetrical device structure. An areal capacitance of >3.8 mF/cm2 was successfully achieved for the SC device. The multifunctional textile OPVs and SCs fabricated herein exhibits underlying potential for next-generation optoelectronics application.

E.PYSF.5
17:30
Authors : Chulhee Lee, Taeyoung Song, Im Tae Hong, Keon Jae Lee, Duk young Jeon
Affiliations : Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST)

Resume : A typical method to increase photoluminescence efficiency is shell formation on quantum dots (QDs) cores for removing surface trap sites. However, insulating property of QDs shell hinders efficient hole and electron injection between shell-coated QD layer and adjacent transport layers leading to decreased device efficiency when the QD layer is applied in optoelectronics. To solve this issue, thermal annealing is usually adapted to increase photoluminescence efficiency by controlling optimum amount of defect sites. On the other hands, relative high temperature used during thermal annealing may cause deterioration of interface between core and shell of QDs. To avoid the deterioration of the interface, Intense pulse light(IPL) annealing can be introduced as an alternative to thermal annealing as it almost instantaneously supplies heat within very short time and brings enough energy to QDs. In this study, we aim to maximize the photoluminescence efficiency of the QDs by controlling the defect sites of the core through a IPL annealing technique while maintaining the interface between core and shell of QDs intact. IPL annealing technique is possible to efficiently remove the defect sites by applying optimum amount of heat to quantum dots core using very short pulse. It is because IPL annealing can avoid over heating on QDs very easily by controlling pulse width. We have observed considerable improvement of quantum efficiency of the QDs by using the afore-mentioned IPL annealing process.

E.PYSF.6
17:30
Authors : Donata Kuczyńska, Agata Sotniczuk, Piotr Kwaśniak, Halina Garbacz
Affiliations : Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland

Resume : The long-term integration of the implants with human body depends on the chemical and physical surface properties of the substrates. Therefore, surface engineering of biomedical materials, is consider to be major technique for improving processes on the implant/body interface. A technique that can modify the titanium possessing initial, not flat topography is Direct Laser Interference Lithography (DLIL), without the deformation thereof. It can generate complex types of texture with high resolution, resulting in micrometer size patterns. Expect for modifying the surface appearance, laser irradiation, with the specific thermal characteristic, can generate appropriate microstructures below the free surface including nanocrystalline or metastable phases. The dissolution of the light elements, such as oxygen or nitrogen, strongly depends on the interactions between the laser, substrate and the atmosphere. The mentioned effects are especially important in the case of the processes limited to the substrate surface. This is of great importance in biomedical applications, where the assessment of various processes such as, protein adsorption, cell adhesion or corrosion resistance is not limited to topographical parameters but includes the formation of oxide layers and microstructural changes. Therefore, this study deals with a careful cross-section microstructural analysis by combining complementary methods such as FIB, SEM, EDS and STEM on different scales to elucidate the microstructure and surface chemistry after laser- patterning and the correlation with phase composition. The gained results are important with regard to the evaluation of laser patterned surfaces in biomedical applications. The description of the microstructure and chemical composition of the DLIL modified titanium samples, can be useful when understanding and characterizing the processes on the bone/implant interface. This research was financially supported by The National Science Centre Poland under Grant no. 2016/23/N/ST8/02044.

E.PYSF.7
17:30
Authors : Jeehun Park, Doewon Moon, Junsang Doh
Affiliations : Pohang University of Science and Technology, Korea

Resume : Dynamic adhesion and detachment of subcellular regions occurs during cell migration. Migrating cells exhibit highly polarized morphology with non-uniform distribution of adhesions across the cells. To systematically investigate roles of adhesions in each region of cells, we devised a new method enabling us to detach defined sub-cellular regions of cells and observe cell responses in real time using a cell-friendly photoresist poly(2,2-dimethoxy nitrobenzyl methacrylate-r-methyl methacrylate-r-poly(ethylene glycol) methacrylate) (PDMP).[1] Unlike conventional photoresist polymers, PDMP dissolves in cell culture medium with near neutral pH conditions after brief UV exposure without harming cells [2], thus in situ micropatterning can be performed in the presence of cells. First, PDMP polymer thin films formed by spincoating on glass coverslips were briefly treated with air plasma and coated with fibronectin. Then, cells were attached on fibronectin-coated PDMP thin films. By using a spatial light modulator, we illuminated UV to specific sub-cellular regions to selectively dissolve PDMP thin films underneath the cells, resulting in detachment of cell adhesions on UV-illuminated regions. Using this new method, we systematically investigated how cells respond when specific sub-cellular regions of the cells were detached.

E.PYSF.8
17:30
Authors : Qianqian Niu, Yaopeng Zhang
Affiliations : State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, Shanghai, 201620, China

Resume : Silk is a kind of natural macromolecule material with good mechanical properties, and good biocompatibility. The mechanical properties of silk based materials are influenced by hierarchical structure, such as molecular, β-crystalline, and nanofiber structure. Moreover, fibrillar structure, especially at nanoscale, has a crucial effect on the excellent performance of native silk. The properties of silk-based materials are originated from the special arrangement at the mesoscale of hierarchical structures. Conventional ways of preparing silk nanofibrils have many disadvantages, such as, toxic solvent, unstable silk nanofibrils, and low productive rate. And the research of silk nanofibrils is not detailed. To fabricate stable SNFs suspension efficiently, novel solution systems were used. Transmission electron microscope, atomic force microscope, and synchrotron radiation small angle X-ray scattering were applied to confirm the size of SNFs accurately. Molecular dynamics simulations of silk models demonstrated that the potential of mean force required to break the HBs between silk fibroin chains or the Van der Waals’ interactions between β-sheet layers in a particular solvent system. Mesoscale research has an important value on the forming and application of materials and the study of silk nanofibrils has guiding significance to form silk-based materials and construct the hierarchical structure model.

E.PYSF.9
17:30
Authors : Zhi Geng, Bo Zhu
Affiliations : State Key Lab for Modification of Chemical Fibers and Polymer Materials& College of Materials Science and Engineering, Donghua University; College of Materials Science and Engineering, Shanghai University

Resume : Poly(3,4-ethylenedioxythiophene) (PEDOT) materials was considered as star materials for fabricating bioelectronic devices due to its excellent electrical conductivity and electrochemical stability. Decoration with functional groups and introducing nanostructures would enhance the electric and biological properties of PEDOT materials, which could expand the potential applications in the fields of bioelectronics. Therefore, it was important to design simple but controllable nano-assembling methods for functionalized PEDOT materials. Compared to traditional template assembling, template-free method had the beneficial of easy fabrication in large scale and integrated preservation of achieved nanostructures, which would make it possible for fabricating nano electronic devices in wafer-scale. Several methods had been reported to realize nano assembling PEDOT materials, however, few of them could realize fabricating different types of nano-morphologies for various functionalized EDOT monomers with one simple method. Herein, we proposed template-free method for fabricating various functionalized PEDOT thin films with nanodot and nanotube morphologies via electrochemical depositing. The morphology parameters could be tuned including diameter, length and density. Glancing incident wide angle XRD was measured to testify the difference of crystal orientation for the achieved nanodot and nanotube thin films. Meanwhile, possible formation reason and process of nanodot and nanotube morphologies were proposed. Besides, simulation tests were carried out and verified the beneficial of the PEDOT and functionalized PEDOT thin films with nanotube morphology using in bioelectronic devices. We hoped the achieved template-free assembling method could be expanded to other kinds of bio-functionalized PEDOT materials and designed PEDOT thin films with proper bio-functional groups and 3D nanomorphologies based on the requirements in practical application of bioelectronic devices.

E.PYSF.10
17:30
Authors : Hiromasa Murata, Yoshiki Nakajima, Takashi Suemasu, Kaoru Toko
Affiliations : University of Tsukuba

Resume : Multilayer graphene (MLG) on insulators will lead to various advanced electronic devices. Graphene has a unique two-dimensional structure, whose characteristics are anisotropic. In line with this, large-grained, highly oriented MLG on insulators has been widely investigated. We previously reported the formation of large-grained, highly-oriented MLG on insulators at low temperature by metal-induced layer exchange. However, it was still not clear what type of metal induces the layer exchange growth of MLG. This study investigates the effect of the species of transition metal in the layer exchange growth of MLG. 50-nm-thick metal (Fe, Co, Ni, Ru, Ir, Pt, Ti, Mo, Pd, Cu, Ag, or Au) and 75-nm-thick amorphous carbon (a-C) thin films were prepared onto a glass using magnetron sputtering. The samples were annealed at 600-1000 °C for 1 h. The metal layers were then etched away. Interactions between transition metals and a-C were classified into 4 groups: (1) Layer exchange (Fe, Co, Ni, Ru, Ir, Pt), (2) Carbonization (Ti, Mo), (3) Local formation of MLG (Pd), and (4) No reaction (Cu, Ag, Au). Thus, the layer exchange was achieved for late transition metals. The metals in group (1) were divided into 2 types: low temperature growth or high crystallinity growth of MLG. Pd has an intermediate characteristic because it is located in the middle in the early and late transition metals. The guidelines for selection of catalyst metal species in the layer exchange growth of MLG will be presented based on the periodic table.

E.PYSF.11
17:30
Authors : Hojae Lee, Wonil Kim, and Insung S. Choi*
Affiliations : Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Korea

Resume : Fe-tannin species have widely been used in our daily life, from food to anti-corrosive products, and recently applied to the interfacial coating with characteristics of non-specific adhesion. Inspired by iron gall ink, a facile strategy was developed for fabricating freestanding films and surfactant-free oil-in-water emulsions through stepwise assembly of Fe(II)-tannin complexes. Fe(II) cations make soluble mono-catecholate complexes with tannins in aqueous solution, and are subsequently oxidized and self-assemble to Fe(III)-tannin complexes for liquid-interface coating. The difference in binding constants of Fe(II)-tannin and Fe(III)-tannin facilitates the oxidation step by lowering the reduction potential of Fe(II). This work provides an insight into the stepwise control of metal-organic complexes for nanobiomedical and nanobiotechnological applications.

E.PYSF.12
17:30
Authors : K. Brassat, A. Taube, D. Kool, L. Tasche, K. P. Hoyer, M. Schaper, J. K. N. Lindner
Affiliations : Nanostructuring, Nanoanalysis and Photonic Materials group, Dept. of Physics, Paderborn University, Germany; Center for Optoelectronics and Photonics Paderborn CeOPP, Paderborn, Germany; Lehrstuhl für Werkstoffkunde, Dept. of Mechan. Engineering, Paderborn University, Germany; Institute for lightweight design with hybrid systems, Paderborn, Germany

Resume : Additive manufacturing allows for custom-made design of implants and medical devices. Here, the Ti-6Al-4V alloy, which is known to show good biocompatibility, is exploited as printing material of in-vivo used elements. Besides the general materials biocompatibility, the control of cell adhesion on a surface depends strongly on the surface morphology. In this paper, we present an interdisciplinary approach combining the expertise of mechanical engineering and nanotechnology to face this problem on different size scales. On the one hand, we investigate a tailored macroscopic architecture of an additively manufactured element. The freedom of 3D device architecture allows for a local adjustment of the mechanical stability while providing lightweight design. Experimental results and numerical simulations for design rules for an optimum device performance are presented. On the other hand, we show an approach to control the cell-implant interactions on a nanopatterned 3D printed Ti-6Al-4V surface. To this end, we present results on the creation of ordered nanopore arrays by self-assembly processes in block copolymer thin films. The block copolymer lithography allows for large-area surface patterning with sub 20 nm features, which are in particular interesting as the nanopore diameter matches the size of the focal adhesions of a cell allowing for the control of cell adhesion. Both aspects together, we sketch an overall approach for improved implant performance bridging size scales from centimeters to nanometers.

E.PYSF.13
17:30
Authors : L. Drbohlavová, A. Talbi, V. Mortet
Affiliations : Institute of Physics, Academy of Sciences Czech Republic v.v.i, Prague 8, Czech Republic, Czech Technical University, Faculty of Biomedical Engineering, Kladno, Czech Republic; Univ. Lille, Centrale Lille, UVHC, ISEN, LIA LICS/LEMAC - IEMN UMR CNRS 8520, F-59000; Institute of Physics, Academy of Sciences Czech Republic v.v.i, Prague 8, Czech Republic, Czech Technical University, Faculty of Biomedical Engineering, Kladno, Czech Republic

Resume : Nowadays the detection of pathogens is an inherent part of environmental or food industry safety. In spite of good selectivity of conventional methods, they are time-consuming and labour intensive. Biosensors are good candidates for real-time monitoring and fast detection of pathogenic agents ‎[1]. Acoustic devices are in the focus of researchers for bio-sensing applications using appropriate surface functionalization ‎[2]. Love wave surface acoustic wave (LW-SAW) sensors possess high sensitivity in liquid ‎[3]. Acoustic energy is confined in the guiding layer close to the sensitive surface, and the energy is not radiated in the liquid due to using pure shear wave with displacement parallel to the surface ‎[4]. Integration of diamond layer brings many favourable properties such as biocompatibility, various biomolecules attachment and prolonged stability of attached biomolecules ‎[5]‎[6]‎[7]. For these reasons, we theoretically investigated properties of layered structures Diamond/SiO2/36°YX LiNbO3 and Diamond/SiO2/ST-cut quartz for potential biosensor applications. Theoretical calculations were carried out with normalized thickness hSiO2/λ in the range of (0.01 – 1) and different thicknesses of the diamond coating. Legendre and Laguerre polynomial approach of wave propagation in layered structures ‎[8] was used for determination of the phase velocity vp and electromechanical coupling coefficient K2 dispersion curves. We also investigated the sensitivity of Diamond/SiO2/ST-cut quartz and Diamond/SiO2/36°YX LiNbO3 structures by using 100 nm thick PMMA film surface loading. The optimal sensitivity of the Diamond/ SiO2/36°YX LiNbO3 is obtained for silicon dioxide normalized thickness hSiO2/λ between 0.3 and 0.6 for all tested diamond thicknesses. In this range of normalized thicknesses hSiO2/λ, the electromechanical coupling coefficient is steeply decreasing from 15 % to 5 %. The diamond/SiO2/ST-cut quartz structure simulation results show similar behaviour. The highest sensitivity and K2 of this structure are obtained for normalized thickness hSiO2/λ between 0.2 and 0.6. Results of this theoretical study show it is possible to fabricate LW-SAW devices with a very thin diamond coating without significant loss of the sensitivity. Properties of LW-SAW devices fabricated on 36° YX LiNbO3 substrate will be presented and compared with theoretical results and previous studies on LW-SAW devices fabricated on ST-cut quartz substrate [9]. [1] SINGH, A. et al., Biosensors and Bioelectronics, 2009, 24(12), 3645-3651. [2] LÄNGE, et al., Analytical and Bioanalytical Chemistry, 2008, 391(5), 1509-1519 [3] GRONEWOLD, Thomas M.A., Analytica Chimica Acta, 2007, 603(2), 119-128. [4] RABUS, D. et al., Journal of Applied Physics, 2015, 118(11), 114505 [5] KRUEGER, Anke a Daniel LANG., Advanced Functional Materials, 22(5), 890-906 [6] Vadym N. Mochalin, et al., Nature Nanotechnology, 7(1):11–23. [7] VAIJAYANTHIMALA, V., et al., Biomaterials, 33(31), 7794-7802 [8] BOU MATAR, Olivier et al., The Journal of the Acoustical Society of America, 2013, 133(3), 1415-1424 [9] Drbohlavová, L. et al., Proceedings 2017, 1, 540.

E.PYSF.14
17:30
Authors : A.N. Kalinkevich, O.V. Kalinkevich, S.N. Danilchenko, M.V. Pogorielov, V.M. Deineka, R.G. Vasyliev, A.M. Sklyar
Affiliations : Institutute for applied physics; Medical Institute of Sumy State University; State Institute of Genetic and Regenerative Medicine; Sumy State pedagogical University

Resume : A series of composite materials based on chitosan were synthesized in order to obtain on the common polymeric basis a set of materials promising soft tissue regeneration, replacing bone defects, regeneration of nerve tissue, as well as materials that could be used as internal hemostatic. The chitosan base was modified by other organic (polyvinyl alcohol) and inorganic (nanocrystalline hydroxyapatite and metal and metal oxide nanoparticles) substances. The materials were characterized by physico-chemical and biological methods, which led to the conclusion that the proposed chitosan-based materials can be used in reconstructive surgery and tissue engineering.

E.PYSF.15
17:30
Authors : Jong Hyun Lee†, Andreas Hadjistylli†, Nazila Kamaly*†
Affiliations : †Department of Micro- and Nanotechnology, Denmark Technical University, 2800 kgs. Lyngby, Denmark Email: nazk@nanotech.dtu.dk

Resume : Atherosclerosis is a systemic and chronic inflammatory condition in which plaques build up inside the arteries. Accumulation of cholesterol in early lesions leads to the formation of macrophage foam cells that ingest free cholesterol, eventually resulting in the presence of intra- and extracellular cholesterol crystal (CC) in advanced atherosclerotic plaques. Over time, growth of the necrotic core leads to plaque destabilization and vessel narrowing, which in turn increases the risk of rupture and thrombosis, leading to heart attacks and strokes. In this work we have developed novel catalytic and anti-inflammatory polymeric nanomedicines that are capable of directly diminishing a major detrimental effect of atherosclerosis; the formation of CCs within plaques. We present the development and characterisation of targeted polymeric nanobiocatalysts capable of selectively targeting atherosclerotic plaques and ‘dissolving’ CCs via a bioinspired catalytic approach based on innate cholesterol catabolic pathways.

E.PYSF.16
17:30
Authors : Salime Bazban-Shotorbani, Nazila Kamaly*
Affiliations : Technical University of Denmark, Department of Micro and Nanotechnology, DTU Nanotech, 2800 Kgs. Lyngby, Denmark

Resume : Atherosclerosis is an inflammation-driven chronic disease of the arteries and the leading cause of death worldwide. Therefore, there is a growing need for efficient drug-testing and drug-screening systems. Commonly used drug-testing technologies are based on two-dimensional cell culture systems, which cannot recapitulate in vivo conditions. On the other hand, animal models are not only lengthy and costly, but also poor predictors of human responses. To overcome these shortcomings, we have proposed to use organ-on-a-chip technology for atherosclerotic nanomedicine studies. We have developed a microfluidic chip consisting of two PDMS layers, separated by a polyester membrane. Each layer has a microfluidic channel, which is capable of simulating the shear condition of a vessel. In addition, endothelial cells were cultured on the membrane and then inflamed to mimic an atherosclerotic vessel. Calcein AM assay was used to investigate cell-viability and morphology of the cells. Moreover, Immunohistochemistry studies and permeation studies were performed. The results of these studies successfully showed tight junctions between cells before inflammation procedure, as well as compromised and leaky junctions after this procedure, which is the main indicator of inflamed vessels. Consequently, the proposed microfluidic chip, which mimics shear condition of a vessel and inflammatory condition of atherosclerosis, is a suitable alternative for typical atherosclerotic drug-screening systems.

E.PYSF.17
 
GENERAL POSTER SESSION with EVENING Party. : Organizers/Chairs: Dr. Oleksandr Ivanyuta, TSN University of Kyiv,Ukraine
17:45
Authors : Vidya N Chamundeswari*, Say Chye Joachim Loo*#
Affiliations : *School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 #Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551

Resume : Electrospinning has emerged as a versatile, cost effective and reliable technique for fabrication of micro/nano fibers and particles with a strict regulation of solution parameters. However, there is minimal evidence till date of any successful dual structured particle on fiber “Sandwich”drug delivery system obtained from electrospinning that could provide controlled release of two or more different drugs. In this study we have successfully fabricated a dual structured electrospun fibrous - micro particle system through electrospinning for delivery of a hydrophilic and a hydrophobic drug simultaneously. A blend of two FDA approved biodegradable polymers was used as the electrospinning solution. Preliminary work focused on optimization of the processing parameters to arrive at 5-8μm porous particles that were electro-sprayed on a fibrous mesh network of PLGA-PCL fibres. The particles were further protected by another fibrous mesh on the top to prevent its loss during post fabrication, thereby the entire system resembling a sandwich. Characterization of the scaffold was carried out using Scanning Electron Microscopy, Rheometer and Fourier transform Infrared Spectroscopy. The hydrophobic Dexamethasone (Dex) was loaded into the fibers while Ascorbic acid (AA) was the hydrophilic biomolecule loaded into the particles. The release profiles of these drugs were quantified with the help of High Performance Liquid Chromatography (HPLC) which enabled the detection of Dex at 246nm and AA at 262 nm respectively. These scaffolds were used to study the differentiation capabilities of mesenchymal stem cells (MSCs) into osteo and chondro lineage. Future studies are directed towards understanding the influence of the carrier morphology and the synergistic effect of multiple biomolecules will be explored in depth for musculo-skeletal tissue engineering.

E.P2.1
17:45
Authors : Vdovenkova T.A.
Affiliations : T.V.A., Saint-Leonard, Quebec, Canada tetyanavdovenkova@cmail.carleton.ca

Resume : AMPA and NMDA synaptic receptor redistribution along neuronal membrane, during Slow Oscillations (SO) of deep sleep accompanied by sigma and ripple action potentials (a. p.), could be the basis for nanoengineering in memory improvement [1]. Calculation of emitted, by accelerated electric charge Q, electric field has shown that linear oscillation of Q = 4x10-7 C during 27.3 min accumulates charge q of cytosolic Na+ ions at axon terminal (q = 20.45x10-5 C/m2 for relative permittivity 384000) sufficient for VGCC activation and, thus, initiates SO a. p. from cortical neuron with 2.5 mm projection of axon along direction of emitted electric field. Subsequent oscillations during 58 ? 77 min simulate nonREM IVth stage. Increase in value of oscillating charge to 35x10-7 C, application of interacting with GABAa receptors sleep promoting medication and ageing accelerate initiation of SO a. p. They are accompanied by sleep spindles and ripple a. p. incorporating synthesized AMPA and NMDA receptors into neuronal membrane for explicit memory improvement. [1] Vdovenkova T.A., Private view on circadian rhythms of brain activity, Symposium "Bioinspired and biointegrated materials as new frontiers nanomaterials VII", 22 ? 26 May, 2017, Strasbourg, France. https://www.european-mrs.com/bioinspired-and-biointegrated-materials-new-frontiers-nanomaterials-vii-emrs

E.P2.2
17:45
Authors : N.Tsierkezos2, U.Ritter2, P.Scharff2, D. Karpenko 3, O. Ivanyuta 1, E.Buzaneva1
Affiliations : 1Taras Shevchenko National University of Kyiv, Faculty of Radiophysics Electronics and Computer Systems, Volodymyrska Str. 64/13, 01601 Kyiv, Ukraine, 2Institute of Chemistry and Biotechnology, 98684, Ilmenau, Germany, PF 100565; 3 NTU of Ukraine “KPI”, Politechnichna14, 03056, Kyiv-56, Ukraine

Resume : The design concept to create the stimuli responsive photoactive at visible range carbon tubes nanostructures is based on constructed molecular models for the photoemission of semiconductor multi-wall carbon nanotubes (MWCNT’s) functionalized by attached to the core and ends photoactive molecular complexes: metal (d - transition metal Cu) - organic (azole ligand) complex or by this complexe with coordinative bonded biomolecule (histidine). The tubes are the building blocks for nanostructures organization due to bonds between the metal ions and the ligands or the histidine molecules at different tubes. The architecture (SEM images) and the photoemission (PL spectroscopy) for nanostructures from these functionalized MWCNT in adsorbed layer at silicon substrate are characterized. The SEM images for this layer are interpreted using proposed molecular architectures models for building blocks connected through Cu2 with attached two ligands molecules at different tubes cores and these bonds are partially retain in the complexes with coordinative bonded histedine molecules that can decrease formation of these structures due to their adsorbing on tubes. These layers with namely one and both two types of nanostructures are characterized by photoemission wide bands, having different intensities and different three subbands in visible range.

E.P2.3
17:45
Authors : N.Tsierkezos2, U.Ritter2, P.Scharff2, D. Karpenko 3, O. Ivanyuta1, E.Buzaneva1
Affiliations : 1 Taras Shevchenko National University of Kyiv, Faculty of Radiophysics Electronics and Computer Systems, Volodymyrska Str. 64/13, 01601 Kyiv, Ukraine, 2 Institute of Chemistry and Biotechnology, 98684, Ilmenau, Germany, PF 100565; 3 NTU of Ukraine ???KPI???, Politechnichna14, 03056, Kyiv-56, Ukraine

Resume : The design concept to create the stimuli responsive photoactive at visible range carbon tubes nanostructures is based on constructed molecular models for the photoemission of semiconductor carbon nanotubes (CNT?s) functionalized by attached to the core and ends photoactive molecular complexes: metal (d - transition metal Cu) - organic (azole ligand) complex or by this complexe with coordinative bonded biomolecule (histidine). The tubes are the building blocks for nanostructures organization due to bonds between the metal ions and the ligands or the histidine molecules at different tubes. The architecture (SEM images) and the photoemission (PL spectroscopy) for nanostructures from these functionalized MWCNT in adsorbed layer at silicon substrate are characterized. The SEM images for this layer are interpreted using proposed molecular architectures models for building blocks connected through Cu2+ with attached two ligands molecules at different tubes cores and these bonds are partially retain in the complexes with coordinative bonded histedine molecules that can decrease formation of these structures due to their adsorbing on tubes. These layers with namely one and both two types of nanostructures are characterized by photoemission wide bands, having different intensities and different three subbands in visible range.

E.P2.4
17:45
Authors : Alina Sionkowska
Affiliations : Department of Chemistry of Biomaterials and Cosmetics, Faculty of Chemistry Nicolaus Copernicus Nicolas University,Torun,Poland, as@chem.umk.pl

Resume : 3D biopolymeric materials based on the blends of collagen (Coll), chitosan (CTS), and hyaluronic acid (HA) were prepared by lyophilization technique. Magnetic particles synthesized by precipitation of iron (II) sulfate heptahydrate and iron (III) chloride hexahydrate in an aqueous solution of chitosan were added to a biopolymer mixture. Dialdehyde starch (DAS) was used as a cross-linking agent for the materials. The structure of the obtained materials was studied using infrared spectroscopy and scanning electron microscope imaging. The properties of the 3D materials such as density, porosity, swelling ability and mechanical properties were studied. It was found that 3D composites made from collagen, chitosan, and hyaluronic acid with magnetic particles are hydrophilic with a high swelling ability (up to 2,646%). Cross-linking of such biopolymeric materials with DAS alters the swelling degree and porosity of materials. The cross-linking process has no significant effect on the density of the materials. The addition of magnetic particles to Coll/CTS/HA materials decreases its swelling ability (1,795% for material containing 30% of magnetic particles) and increases the density of the studied materials. 3D materials based on Coll/CTS/HA with magnetic particles are rigid and inflexible. With the increasing content of magnetic particles in the polymer blend, the Young's modulus decreases. 3D material with magnetite particles can be used in biomedical applications, such as tissue repair and drug delivery.

E.P2.5
17:45
Authors : Shigeori Takenaka
Affiliations : Department of Applied Chemistry & Research Center for Bio-microsensing Technology, Kyushu Institute of Technology, Kitakyushu 804-8550 Japan, shige@che.kyutech.ac.jp

Resume : Potassium ion (K+) plays an important role in many physiological events such as homeostasis in the heart muscle and hyper polarization of neurons and thus it is important to develop not only a detection method for this cation, but also a fluorescence imaging technique, especially that may provide spatiotemporal information on their location in the cell. However, specific detection of K+ is hampered because of the existence of sodium (Na+) and other ions in the cell. Limited methods have been developed to monitor K+ in a living cell. Oligonucleotides with sequences of thrombin binding aptamer (TBA) are known to form tetraplex structures upon K+ ion binding. We successfully synthesized a Fluorescence resonance energy transfer (FRET)-type fluorescent reagent, PSO-5, showing preference for K+ on the basis of a conformational change in TBA sequence. PSO-5 consisted of thrombin binding aptamer (TBA) carrying FAM at the 5?-end conjugated with a peptide carrying TAMRA and biotin at the middle and at its C-terminus, respectively (Fig. 1A). The ternary complex of streptavidin with PSO-5 and biotinylated nuclear export signal peptide in a 1:1:3 stoichiometry had the dissociation constant of 2.24 mM for K+ and its preference for K+ is 236 times over Na+. K+ in the cell was visualized based on the FRET ratio change of this complex. FRET signal of PSO-5 decreased upon addition of amphotericin B and ouabain which facilitate K+ efflux from the cell, which shows decreasing K+ concentration in the cell {1,2}. As a next step, we tried to design the reagent carrying higher preference for K+ over Na+ and to visualize intercellular K+ concentration gradient on the cell surface using it. One of the successful approaches was the random biotinylation of membrane protein by Sulfo-NHS-biotin and localization of the reagent on the cell surface through streptavidin. [1] Ohtsuka, K. et al., Chem. Commun., 48 (2012) 4749. [2] S. Takenaka, Synthesis of fluorescent potassium ion-sensing probes based on a thrombin-binding DNA aptamer-peptide conjugate, 2015. Curr. Protoc. Nucleic Acid Chem. 62:8.9.1-8.9.9. doi: 10.1002/0471142700.nc0809s62.

E.P2.6
17:45
Authors : Peilin Chen
Affiliations : Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan e-mail: peilin@gate.sinica.edu.tw

Resume : I will discuss recent development in the advanced optical imaging biosystems in our group. The various nanoparticles for sensing the pH value in cellular environment created by us. By surface functionalization schemes, it is possible to control the location of nanoparticles in cells allowing us to track the local pH value around the nanoparticles inside cancer cells. The acidification process of nanoparticles in the endocytosis process can be recorded. Such technique can be extended to detect the local chemical reaction inside living cells. As for in vivo imaging, we have utilized the multi-photon microscopy to investigate the disease models with the help of nanoparticles. I will discuss the applications of multi-photon imaging in various mouse models. On our multi-photon platform, it is possible to track the movement of various types of cells including platelets, white blood cells, red blood cells, tumor stem cells and nanomaterials in mouse models using surface modified nanoparticles at a frame rate as high as 100 frames. References: Kun Hung Chen, David J. Lundy, Elsie K.W.Toh, Chien Hsi Chen, Peilin Chen et al., Nanoparticle distribution during systematic inflammation in size- depended and organ specific, Nanoscale, 2015, 7, 15863.

E.P2.7
17:45
Authors : Sathi Roy1 #, Nancy Mohamed Ahmed Elbaz2 #, Neus Feliu1, Wolfgang J. Parak1*
Affiliations : 1FachbereichPhysik und Chemie, and Center for Hybrid Nanostructure (CHyN), Universität Hamburg, Hamburg, Germany. 2FachbereichPhysik, Philipps Universität Marburg, Germany. #contributed equally

Resume : Development of effective nano-sized drug delivery system has attracted lot of attention in pharmaceutical research. Polyelectrolyte capsules (PEC) made of different biodegradable and non-biodegradable polymers has emerged as a promising candidate for this purpose.1,2 Despite several advantages, the bigger size (>2 µm) of this system restricts their circulation into bloodstream and limits from any real application.3 Here we report a novel biodegradable PEC drug delivery system (?1µm) made of alginate (ALGI) and poly-L-arginine (PARG) with a pH-sensitive outer layer of EUDRAGIT L 100 (EuL) polymer. Curcumin which possesses antioxidant, anti-inflammatory and anticancer activity, has been used as a model drug.4 Encapsulation of curcumin inside this nanocapsule protects their therapeutic activity and also increases bio-availability. Cell viability, uptake and release studies were conducted to confirm their effectiveness as model drug delivery system.

E.P2.8
17:45
Authors : dr inż. Natalia Sienkiewicz, dr hab. inż. Krzysztof Strzelec, prof PŁ, mgr inż. Tomasz Szmechtyk
Affiliations : Lodz University of Technology, Faculty of Chemistry, Institute of Polymer and Dye Technology

Resume : Recently, there is a growing interest in using polymers to obtain materials that have antibacterial and antifungal properties. These materials can be used as antibacterial touch surfaces, in places where hygiene and sterile conditions are particularly required (e.g. in healthcare, cosmetology pharmacology or food industries) and thus can become an alternative to commonly used disinfectants, which mostly show high toxicity to the environment and the human health. This is particularly important because, as research shows, about 80% of infectious diseases are transmitted through touch. The largest clusters of colon Escherichia coli, the stick of pneumonia Klebsiella pneumoniae and Staphylococcus aureus which cause the most common infections in hospitals and other medical places occur on objects located directly next to patients, such as bed rails, chairs, and cabinets so there are constitute tactile surfaces, from which bacteria can easily transfer to patients, employees or other people in hospitals. Therefore, the type of used materials has a great importance in the preparation of final products that should primarily inhibit the growth of bacteria, viruses and fungi. Selected extracts and essential oils will be used as antibacterial, antifungal and antiaging additives for polymers. Our initial studies have shown that the use of turmeric extract in the amount of 50 000 ppm as an antibacterial additive to the foam enabled to obtain ready-made products with antibacterial properties for Bacillus subtilis and Candida albicans. The additional use of the alternative source of natural antioxidants and antimicrobials increased the resistance to aging compared to the standard sample at a level comparable with commercial antioxidants.

E.P2.9
17:45
Authors : dr inż. Natalia Sienkiewicz, dr hab. inż. Krzysztof Strzelec, prof PŁ, mgr inż. Tomasz Szmechtyk
Affiliations : Lodz University of Technology, Faculty of Chemistry, Institute of Polymer and Dye Technology

Resume : It has become very important to find a natural antimicrobial material that can be incorporated into the formulation of polyurethanes and retain its bacteriostatic ability. In our opinion much attention should be focused on polyurethane modification with substances of vegetable origin of a biocidal nature. Plants and extracts obtained from them have been used for centuries in ethnomedicine and for cosmetic purposes. Currently, it is well known that plant-derived components with the strongest biological activity include essential oils and polyphenols, classified as secondary metabolites of plants. Essential oils are characterized by intense fragrance composed of mixtures of volatile compounds, such as terpenes (monoterpenes, diterpenes, sesquiterpenes), terpenoids (isoprenoids) and other aromatic and aliphatic compounds (aldehydes, alcohols, phenols, tannins, esters, ketones). Polyphenols containing pyrogallols showed strong antibacterial activity which could have potent antibacterial activity suitable for commercial use. The assumed research program will go to get foams with antibacterial properties and improvement the anti-aging properties of these materials.

E.P2.10
Start atSubject View AllNum.Add
09:00 PLEANARY SESSION    
12:00 Lunch Break    
 
(project) Collaborative Session E-MRS/Polish Society of Biomaterials: Polish National Project” New materials & technologies for biomedical engineering” : Chair: Prof. Malgorzata Lewandowska-Szumiel
13:00
Authors : Jan Chlopek
Affiliations : Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland, chlopek@agh.edu.pl

Resume : The aim and tasks of the project “New Materials and Technologies for Biomedical Engineering”. Work in progress: new biomaterials designed for the treatment of human tissues and organs.

E.3.1
13:20
Authors : Malgorzata Lewandowska-Szumiel
Affiliations : Tissue Engineering Team, Center of Biostructure Research, Department of Biophysics and Human Physiology, Medical University of Warsaw, Poland, mszumiel@ib.amwaw.edu.pl

Resume : The idea of tissue reconstruction by means of tissue engineering (TE) is extremely up-to-date. One of the TE method is transplantation of patient own cells isolated from the health part of the tissue, proliferated, differentiated in vitro, settled on 3 dimensional (3D) scaffold and stimulated to produce extracellular matrix. In Poland the consortium consisting of 10 institutions has been established in order to elaborate the optimal implantable constructions of this type. Specialists in material engineering representing all biomaterial fields (polymers, ceramics, metals and composites) are represented in the consortium and will work on carriers for cell transplantation. Candidate scaffolds will be tested in vitro in the culture of human cells harvested from bone, cartilage and marrow. Two radiation centers experienced with sterilization of medical products are also involved in the venture in order to resolve problems with sterilization of all the products as well as to use radiation as a method of desirable modifications of material properties. All scaffolds are to be designed in strong collaboration with orthopedic and laryngological surgeons. The main expectation is to work out TE constructions dedicated to the specific clinical situations. Due to the certainly interdisciplinary character of the consortium we do believe to be able to accomplish the result faster then it could be achieved in many small bilateral collaborations.

E.3.2
13:40
Authors : Elzbieta Pamula
Affiliations : Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland, epamula@agh.edu.pl

Resume : Sodium aluminophosphate glasses were evaluated for their bone repair ability. The glasses belonging to the system 45Na2O-xAl2O3-(55-x)P2O5, with x=(3, 5, 7, 10 mol%) were prepared by a melt-quenching method. We assessed the effect of Al2O3 content on the properties of Na2O-Al2O3-P2O5 (NAP) glasses, which were characterized by density measurements, DSC analyses, solubility, bioactivity in simulated body fluid and cytocompatibility with MG-63 cells. To our best knowledge, this is the first investigation of calcium-free Na2O-Al2O3-P2O5 system glasses as bioactive materials for bone tissue engineering.

E.3.3
15:30 Coffee Break    
16:55
Authors : Krzysztof J. Kurzydlowski
Affiliations : Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland, kjk@inmat.pw.edu.pl

Resume : The main objective of the network ?Nanocomposites for Medical Applications? is: to enhance the research potential of all the partners both due to improved efficiency of intellectual work and extended available facilities in the fields: materials science and engineering, biomechanics, biomaterials and medicine.The example of publications is our article ?Architecture and Properties of PUR/Calcite Compose Scaffolds for Bonee Tissue Engineering?

E.3.4
17:15
Authors : Krzysztof Sikorski
Affiliations : Faculty of Materials Science and Engineering, Warsaw University of Technology, Poland, sikorski@inmat.pw.edu.pl

Resume : The project includes optimization of the microstructure of composites for restorative materials in stomatology with the aim to improve mechanical properties and reduce the contraction during polymerization. The following activities are foreseen: development of new composites for stomatology. characterization of microstructure, quantitative description of the microstructure using image analysis and stereological methods, investigations of mechanical properties (tensile and compression test, tribological properties), application of finite elements methods to simulate internal stresses, study of the quality of the junction between restorative materials and tooth tissue, study of the degradation of composite materials in bio-environment. The aim of the work is to develop new composites that better an amalgam will fulfill the requirements for permanent fillings. It is expected that high strength and wear resistant material can be obtained by suitable type and form of reinforcing particles and polymerising contraction could be considerably diminished by appropriate combination of two polymers self compensating volumetric changes during polymerisation. The calculations of the residual stresses (performed by FEM simulation) in the composite together with the quantitative analysis of the microstructure and mechanical testing of the samples could allow to design the optimum microstructure of the composite in question.Various, advanced methods and techniques of material investigations will be used to characterise the behaviour of the composites in the conditions simulating the oral cavity conditions to which PF are exposed to. The contact area between the composite filling and the tooth tissue will be examined from the point of view of the metabolism products promoting of decay. The obtained composites should meet ISO standards. Their clinical studies are planned and the materials developed will be commercialized ones in with pre-selected companies.

E.3.5
17:30
Authors : Joanna Siejka - Kulczyk
Affiliations : Faculty of Materials Science and Engineering, Warsaw University of Technology, Poland, jsiejka@inmat.pw.edu.pl

Resume : The most popular materials currently used for dental fillings are ceramic - polymer composites, which increasingly replace amalgam due to the possible toxicity of mercury and the unpleasant dark color. Resin composites are promising materials, but have some disadvantages. These are: lower than for amalgams mechanical parameters and significant polymerization shrinkage. These inconveniences can be limited by the microstructure optimization. Such studies have been carried out at the Faculty of Materials Science and Engineering of Warsaw University of Technology. Fabricated materials consist of polymer matrix based on acrylic resins and ceramic glass fillers. Fillers are characterized by various particle size distributions and chemical compositions. Average particle size of powders differs by 3 orders of magnitude. Three fractions of powders were used. It is well known that adding of ceramic fillers improve both mechanical and physico ? chemical properties. From the microstructure optimization point of view, it seems that the addition of powders which differ in particle size is the most advantageous. However, the good adhesion between polymer and ceramic phases has to be ensured. Thus, the fillers are silanized in order to obtain better bonding with the polymer matrix. Nevertheless, good joint between organic and inorganic phases is still a ?week point? of fabricated composites. In particular, nano ? filler can agglomerate when its volume fraction is above the critical value. This may lead to a substantial decrease of mechanical properties. This report views the effect of microstructure on selected mechanical (flexural strength, micro - hardness) and physico ? chemical (polymerization shrinkage, water sorption) properties.

E.3.6
17:40
Authors : Roman Major1, Malgorzta Gonsior2, Marek Sanak3, Roman Kustosz2, Juergen M. Lackner4
Affiliations : Institute of Metallurgy and Materials Science, Polish Academy of Sciences, Reymonta St. 25, Cracow, Poland, r.major@imim.pl

Resume : The, main goal of the work was to develop novel blood contacting composite materials for the re-design of the flexible mechanical ventricle assist device (VAD) heart valves. The idea was to elaborate metal-reinforced polymer composites. The studies performed have lead to the material selection for the surface modification of metallic scaffold. Haemo-compatible, biofunctional, ultra-elastic, thin carbon-based coatings were proposed. The surface was designed in order to eliminate thrombogenic and microbial construction by reduction of turbulence and sufficient washing of biofunctional-adapted surfaces and reduce heart assist costs allowing broad use for temporary heart support. A classical instrumentation for the dynamic test of hemocompatibility involves a flow chamber with a contact surface between blood stream and tested plate. In the current study we investigated a simplified model of the whole blood shear stress, based on a cone and plate rotational viscometer. Several indices of platelet activation were analyzed, including platelet- and granulocyte-platelet aggregates, platelet activation markers and platelet-derived microparticles. This model allowed to estimate platelet destruction, however no adhesion could be measured directly. In following tests of several polymer and metallic layer coated materials, the test revealed comparable performance to more laborious hemocompatibility experiments. Acknowlegement The main theme of the work concerns the statutory works of the Institute of Metallurgy and Materials Science PAS ?Z-2? and applied research project: ?Nonthrombogenic metal-polymer composites with adaptable micro and macro flexibility for next generation heart valves in artificial heart devices?. This project is implemented under the Program for M-ERA.NET Transnational Call 2014, according to the agreement DZP/M-ERA.NET-2014/291/2015, funded by the National Centre for Research and Development

E.3.7
19:00 E-MRS Fall Meeting Evening Party    
Start atSubject View AllNum.Add
 
One Day Session:Towards novel concepts in biomaterials/structures/interfaces systems sciences & technologies: Imaging Life at the atomic/nanoscale dedicated to the 2017 Nobel Laureates in chemistry Professors : Jacques Dubochot, Joachim Frank, Richard Henderson “for the development of cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution”. Organizer/Chair Prof. Peilin Chen, Taiwan
09:45
Authors : Ioan Andricioaei
Affiliations : University of California, Irvine, California 92697, USA andricio@uci.edu

Resume : Dynamics and thermodynamics of the interaction of carbon nanotubes with DNA and their effects on DNA properties The interaction of single-walled carbon nanotubes (SWNTs) with DNA is crucial for several nano-materials applications, including SWNT:DNA nanoscale devices or nanosized building blocks for use in nano-switches and nanoscale wiring. In biotechnological applications, modulating the DNA:SWNT interaction is important for SWNT purification, DNA recognition, and ultrafast DNA sequencing, and drug delivery. I will present a study of the the conformational equilibrium and the dynamics between B-to-A forms of double-stranded DNA adsorbed onto single-walled carbon nanotubes (SWNT) using free energy profile calculations based on all-atom molecular dynamics simulations. The potential of mean force of the B-to-A transition of ds-DNA in the presence of an uncharged (10,0) carbon nanotube for two dodecamers with poly-AT or poly-GC sequences is calculated as a function of a root-mean-square-distance metric quantifying the B-to-A transition. The calculations reveal that in the presence of a SWNT DNA favors B-form DNA significantly in both poly-GC and poly-AT sequences. Furthermore, the poly-AT DNA:SWNT complex shows a higher energy penalty for adopting an A-like conformation than poly-GC DNA:SWNT by several kcal/mol. The presence of a SWNT on either poly-AT or poly-GC DNA affects the free energy of the transition such that the B form is favored by as much as 10 kcal/mol. The presence of the marked structural differences between B- and A-DNA have consequence for understanding thermal and conduction properties of composites of nanotubes and DNA used in biomaterials applications.

E.4.1
10:30 Coffee Break    
11:30
Authors : S. Osella, B. Trzaskowski
Affiliations : Chemical and Biological Systems Simulation Lab, Centre of New Technologies, University of Warsaw, Banacha 2C, 02-097 Warsaw, Poland.

Resume : In the last five years, a lot of research effort has been devoted to the creation of hybrid materials which change the electronic properties of one constituent by changing the optoelectronic properties of the other one. The most appealing approach consists on the interaction between organic materials or metals with biological system such as proteins or DNA. Although experimental efforts have already resulted in the formation of a number of stable hybrid bio-organic materials, the main bottleneck of this research field is the formation of the interface between the biological part and the organic/metal one. In particular, the efficiency of the final devices is very low due to problems with the interfacing of such different materials, charge recombination at the interface and the high possibility of losing the function of the biological component which leads to inactivation of the device. Here, we present a multiscale computational design which allow the study of complex interfaces for stable and highly efficient hybrid materials for biomimetic application. In particular, we focus on the use of graphene as organic material/metal and light harvesting protein complexes (Photosystem I) as biological counterpart, linked together via a self-assembly monolayer (SAM) and a biological linker (cytochrome C) to allow flexibility of the whole system, in order to create novel biomimetic materials for solar-to-fuel, bio-transistors or bioorganic electronic applications.

E.4.2
12:00
Authors : Peilin Chen
Affiliations : Research Center for Applied Sciences, Academia Sinica

Resume : In this lecture, we will present the recent developments in our group related to circulating tumor cells (CTCs). We will first discuss the utilization of multi-photon microscopy for monitoring CTCs in the blood stream with the help of quantum dots. CTCs are cancer cells that break away from a primary tumor or metastatic site, escape from immunosurveillance, and then circulate in the peripheral blood with the capability of forming distant metastases. The number of CTCs has been used as an indication for the progress of tumor state. However, how CTCs travel in the bloodstream and how they crossed the endothelial barrier are not known. In our group, we have utilized the multi-photon microscopy to study CTCs noninvasively. Pancreatic cancer cells expressing fluorescence were subcutaneously injected to the earlobes of mice forming solid tumor. When the cancer cells break away from the tumor mass, the cancer cells in blood stream can be monitored. The number of CTCs observed in the blood vessels near the tumor mass increased to a maximum value after five week of inoculation. We also tried to identify a sub population of CTCs such as cancer stem cells (CSCs). The trajectories of CTCs and CSCs were measured and analyzed.

E.4.3
13:00 Lunch Break    
14:00
Authors : Vicki Wing-Kiu Yeung, Ji-Yen Cheng
Affiliations : a Research Center for Applied Sciences, Academia Sinica, Taiwan b Institute of Biophotonic, National Yang-Ming University, Taipei, Taiwan c Department of Mechanical and Mechantronic Engineering, National Taiwan Ocean University, Taiwan

Resume : Medical decision based on single biomarkers often results in a high possibility of false positives and false negatives. The combination of multiple biomarkers improves accuracy of disease diagnosis. This demonstrates the importance of developing sensitive multiplexing assay of biomarkers to improve reliability of diagnosis. Nano-structure transmission SPR is an emerging technology with potential for multiplex detection and development of POC testing platforms. SPR sensors based on nanostructures facilitate chip-scale integration and allow high-throughput and label-free detections of biomarkers. Nanostructure SPR substrates can be fabricated with inexpensive and high-yield techniques and are therefore suitable candidates for low cost mass production and integration of disposable sensor cartridges. Multiple arrays can be fabricated and by immobilizing different recognition molecule, each array can be used to probe specific target molecule and can be interrogated separately. In this work, we demonstrate a method that employs gold nanoslits (GNS) surface plasmon resonance (SPR) sensor integrated in a microfluidic chip for multiplex detection of miRNA biomarkers. An array of nanoslit structures was designed and fabricated on an olefin polymer film using nanoimprint lithography and 80 nm gold was deposited. The approach for detection include double hybridizations by the capturing probe I on the Gold nanoparticles (AuNPs) and the second capturing probe II immobilized on the GNS array. The double hybridization with two probes complementary to the target molecules results in high specificity of detection. AuNPs used for the signal enhancement of SPR. In this work, target miRNA as low as 100 fM were detected simultaneously without any labeling and amplification. The high sensitivity of this method is attributed to the AuNPs-enhanced transmission SPR on the gold nanoslits through increased surface mass, high dielectric constant of Au particles and electromagnetic coupling between AuNPs and the extraordinary optical SPR transmission of gold nanoslits.

E.4.4
15:30 Coffee Break    

Symposium organizers
Arzum ERDEMEge University

Analyt. Chem. Dep., Faculty of Pharmacy, 35100 Bornova, Izmir, Turkey

arzume@hotmail.com
Eugenia BUZANEVATSN University of Kyiv, NASU

The Scientific and Training Centre “Physical and Chemical Material Science”, Volodymyrska Str. 64/13, 01601 Kyiv, Ukraine

emrs@univ.kiev.ua
Peilin CHENResearch Center for Applied Sciences

Academia Sinica, Taiwan, 128, Sect.2 Academia Rd, Taipei, Taiwan 115

peilin@gate.sinica.edu.tw
Peter SCHARFFTechnical University of llmenau

Institute of Chemistry and Biotechnology, Weimarer Strasse 25, 98693 Ilmenau, Germany

peter.scharff@tu-ilmenau.de