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2015 Spring

Organic and Bio-materials

V

Bioinspired and Biointegrated Materials as Frontiers Nanomaterials V

Focus on advanced biotechnologies ( bio - molecular inspiration, - mimetic synthesis, - replication, - immobilization, - templating, - molecular imprinting) in healthcare and environmental technologies of nano - materials, - devices, - robotic systems

“Bioinspired and Biointegrated Materials as New Frontiers Nanomaterials I, II, III, IV ” Symposia were successful events due to presented reports “nanoscientists, inspired by nature”, and discussions on advanced nanomaterials future developments to the “hot” field natural inspiration and 3D printing of bionanomaterials and newest nanotechnologies of these nanomaterials and based on ones bio – electronic, - photonic, - magnetic systems for Healthcare fields.

We are honored to announce the 2015 year’s symposium what is aimed to give overview of multifunctional applications in biomedical healthcare field and environmental biotechnology of developed processes bio - inspiration, immobilization, templating, mimicking, imprinting of nanomaterials, -systems, -robotic devices. This is newest nanobio -materials, -systems, -robotic devices field, which determines developing biomimetic cells and skin, bone tissue engineering, remodeling ones and adaptation to a regeneration of neural systems using, created implantable bionic systems.

 

Scope:

 

This symposium will cover the frontiers on the design, researching, engineering molecular scale characterization multifunctional biomolecular systems in medical and environmental researches; bio -photonics, -electronics, -magnetic molecular systems and bioimmobilized nanoparticles as nanorobots in bionanomedical applications in vivo, using bioinspired, mimetic, templated by biomolecules (virus, marine plants proteins) inorganic nanoparticles for the quantum dots nanosystems and nature and grown nanofibres for bioinspired composite materials.

The symposium will bring together researchers from physical, chemical sciences; bioscience, biobrain processing modeling and nanotechnology to discuss the latest advancements.

Proposed subjects for presentations and discussions at the May 11 to 12-15 SESSIONS have actuality for Investigators of lasted the EU MPNS COST Actions on 2013 – 2017, for example, the Action MP 1301 “New Generation of Biomimetic and Customized Implants for Bone Engineering (NEWGEN) and the Action MP 1005 ”From nano to macro biomaterials (design, processing, characterization, modeling and applications to stem cells regenerative orthopedic and dental medicine (NAMABIO) and the Action MP 1206 “Electrospun Nano-fibes for bioinspired composite materials and innovative industrial applications”.

Tentative list of Invited Collaborators as Organizers – Chairs – Speakers, and the Scientific Committee Members to five days Sessions:

The May 11 to 12, Two Day Session: “Biological and Bio -mimetic, -synthesized supramolecular polymers and molecular imprinting cells, tissues, scaffolds grown and mimetic surface, interface organization. Nanocharacterization.”

Invited to collaborate:

Frederic Guittard’s Group Surfaces and Interfaces; Joao F. Mano, 3B’s Research Group Biomaterials, Biodegradables and Biomimetics; Haupt’s Research Group on Biomimetic Polymers, Molecular Imprinting, and Nanostructuration; Masaru Tanaka’s Research Group Biomaterials. Cell Engineering; Insung S. Choi’s Lab., Center for Cell-Encapsulation Research; Duncan S. Sutherland Research Group Nanobiointerfces; Johannes Heitz’s Research Group Laser Assisted Nanotechnologies, Emmanuel Stratakis, Research Group Micro/nano – structures biomimetic surfaces for neural cells grown in scaffolds, Hiroshi Endo, Bioinspired surface topologies technologies: two types of superhydrophobic; Mikaeyel Aznauryan, Single-molecular probing in vivo and viro; Andreas Bo O. Dahlin, Bionanophotonics; Yoshikatsu Akiyama, Biosurface/Interfaces.

The May 13, One Day Session “Biogenic, Bio - mimetic minerals, -hybrid supramolecular materials and - activated metal, metal hybrid surfaces: a recrystallization at biointerfaces in living cells and with natural bone and bone regeneration”.

Invited to collaborate:

The EU MPNS COST Action MP 1301 “New Generation Biomimetic and Customized Implants for Bone Engineering” (NEWGEN): Francis Cambier, Belgium Ceramic Centre, Mons, Belgium; Paolo Palmero, Politechnic de Torino, Italy; Lorin Michael Benneker, University of Bern, Inselspital University of Bern, Switzerland; Maria Luisa Ferrer, Mineralization/Biomineralization: 3D scaffolds. ICMM, Spain; Osamu Suzuki,Tohoku University, Japan; V. Dubok, IPMS,NASU, Kyiv, Ukraine; Julian R. Jones’s Group on Glass Scaffolds, Dep.of Materials, Imperial College, London, UK; Lia Rimondini, Sccafolds for natural polymers, Universite del Piemonte Orientale “Amedeo Avogadro”, Italy.

The May 14, One Day Session ”Bio - synthesized, -templated, -immobilized inorganic nanoparticles, nanocarbon molecules in complex nanomaterials: fundamentals and biomedical functions”. Special Invited: Fumio Watari, Nano Biomedical Society, Hokkaido University, Sapporo, Japan; S. Wong, Brookhaven Nat.Lab.,USA.

Invited to collaborate the Working Groups:

Simon Robert Hall “Biosynthesis processing” Complex Functional Materials Group, University of Bristol, UK; Daria C. Zelasko-Leon, Philip B. Messesrsmith’s Group Biomedical Engineering, Northwestern University,USA; Genevieve Pourroy, Fundamentals and Biomedical Functions of Magnetic Nanoparticles Chemistry, CNRS, IPCMS, University of Strasbourg, France; L.Reznichenko, The working team Biomedical nanomaterials. Institute BioColloidal.Chemistry, Ukraine.

The May 15, One Day Session “Biological and biomimetic single, supramolecules and biotemplated quantum dots as photonic, electronic, magnetic molecular systems in cells: fundamentals and molecular processes in cells. Discussion on Organic - and Bioorganic – electronics, - photonics - magnetic systems and smart interfaces biomedical frontiers”.

Invited to collaborate:

Cordt Zollfrank’s Biogenic Polymers Group and Daniel Van Opdenbosch, Research group “Holographic patterning of functional materials using phototactic microorganisms”, Germany; Bo Zhu, State Key Lab. For Modification of Chemical Fibers and Polymer Materials, Donghua University, China; Victoria Birkedal’s Group Single Molecule Photonic, iNANOand CDNA, Aarhus University, Denmark; Arzum Erdem, DNA Chip and the disposable genosensor, Ege University, Turkey; Beate Strehlit’s Working Group Biological field measuring methods, UBZ, Germany; Uwe Ritter, Andreas Schober and Nikos Tsierkezos, Institute for Chemistry and Biotechnology, TU Ilmenau, Germany; Friedrich C. Simmel’s Lab “Physics of Biomolecular Systems”, TU of Munchen, Germany; Shutao Wang, Lab. of Bioinspired Smart Interface Science, Technical Institute of Physics and Chemistry of the Chinese Academy of Sciences, Beijing, China; Ovidiu Crisan, Magnetism and Superconductivity Lab. National Institute for Materials Physics, Bucharest –Magurele, Romania. Peilin Chen, Research Center for Applied Sciences, Academia Sinica, Taiwan; Andreas J. Mason and Wei Li , Implantable Sensors, Michigan State University, USA; Ling Fu, Huazhong University of Science and Technology, Medical Optoelectronics in Wuhan National Lab. for Optoelectronics, China and Damian Bird, Universal Biosensors Pty Ltd, Victoria, Australia, Femtosecond Biophotonics.

A special Invited Young Researcher Session for young scientists and graduate students’ talks is planned in the symposium’s first day, May 11, on 5-7 p.m. (Invited Organizer – Chair MSc, Researcher Oleksii Dubok, TSN University of Kyiv, IPMS, NASU, Ukraine).

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Special ONE DAY Session “Bio –molecular inspiration, -mimetic synthesis of multifunctional nano –materials, -systems”. Invied chairs : Prof. Neil Tomas University of Nottingham, Centre for Biomolecular Sciences, School of Chemistry, neil.thomas@nottingham.ac.uk; Dr. Erik Daniel Glowacki, Linz Institute for Organic Solar Cells (LIOS), Linz Austria; eric_daniel.glowacki@jku.at; Prof. Bo Zhu, State Key Lab for Modification of Chemical Fibers and Polymer Materials & College of Materials Science and Engineering, Donghua University, Shanghai, China, bzhu@dhu.edu.cn. Assisted by PhD Jeanne Bernadette Tse Sum Bui, Compiègne University of Technology, CNRS Enzyme and Cell Engineering Laboratory, France; jeanne.tse-sum-bui@utc.fr
08:40
Authors : Prof. Karsten Haupt (karsten.haupt@utc.fr)
Affiliations : Compiegne University of Technology, CNRS Institute for Enzyme and Cell Engineering, Compiegne, France

Resume : The technology of molecular imprinting allows for the preparation of synthetic polymers with specific binding sites for a target molecule. This can be achieved if the target is present during the polymerization process, thus acting as a molecular template. Monomers carrying certain functional groups are arranged around the template through either noncovalent or covalent interactions. Following polymerization with a high degree of cross-linking, the functional groups are held in position by the polymer network. Subsequent removal of the template by solvent extraction or chemical cleavage leaves cavities that are complementary to the template in terms of size, shape and arrangement of functional groups. These highly specific receptor sites are capable of rebinding the target molecule with a high specificity, sometimes comparable to that of antibodies. Molecularly imprinted polymers have therefore been dubbed "antibody mimics". It has been shown that they can be substituted for biological receptors in certain formats of immunoassays and biosensors. They have also been used as stationary phases for affinity separations, for the screening of combinatorial libraries, and as enzyme mimics in catalytic applications.

V.1-.1
09:00
Authors : Thomas J. Webster
Affiliations : Department of Chemical Engineering, Northeastern University, Boston, MA 02115; th.webster@neu.edu

Resume : This presentation will focus on the unprecedented impact nanotechnology has had across all of medicine, specifically focusing on improved disease prevention, diagnosis, and treatment. Disease systems will cover cancer, infection, inflammation, and poor tissue growth including bone, vascular, cardiovascular, bladder, nervous system and others. A particular focus will be placed on sensors that personalize medicine towards one's immune system. Toxicity will also be covered. A new field, picomedicine, will also be introduced concerning how further advances in medicine can be made. Here, the focus will be placed on controlling electron distributions (as opposed to atomic distribution in nanotechnology) to improve disease prevention, diagnosis, and treatment.

V.1-.2
09:30
Authors : Dr.Simon R. Hall (presenter), David C. Green, Rebecca Boston, Jason Potticary, Wataru Ogasawara
Affiliations : School of Chemistry, University of Leeds, Leeds, LS2 9JT, UK; Complex Functional Materials Group, School of Chemistry, University of Bristol, Bristol, BS8 1TS; Department of Bioengineering/Bioenergetics, Nagaoka University of Technology, 1603 1 Kamitomioka, Nagaoka, Japan; simon.hall@bristol.ac.uk

Resume : Scalability in the synthesis of high temperature superconductors can be achieved via a biotemplated sol-gel synthesis. On calcination, the individual metal salts undergo multiple chemical transformations before finally reacting to form the correct stoichiometry. The precise mechanism of sol-gel synthesis of Bi2Sr2CaCu2O8+x (Bi-2212) is to-date unknown. Synthetic protocols invariably result in the formation of large crystallites of SrCO3 (decomp. 1,494 °C) that are stable throughout calcination (typically around 900 °C) altering the final stoichiometry of the superconductor. It has been postulated that calcium may be present in the synthesis at elevated temperatures as calcium carbonate, which aids in the melting of SrCO3 by forming a low-melting eutectic mixture. This would ensure full melting of the strontium phase and its subsequent availability for reaction to form Bi-2212, but this has never been conclusively determined. By employing a biopolymer, which chelates multivalent cations, we can restrict the nucleation of SrCO3 to the nanoscale and easily follow via X-ray diffraction and thermogravimetric analysis, the development of a mixed carbonate eutectic as the synthesis of Bi-2212 progresses. Positive identification of a eutectic-based mechanism of formation enables us to further lower the eutectic melting point by the incorporation of a biopolymer rich in potassium, resulting in the formation of Bi-2212 at 50 K lower than has been previously observed.

V.1-.3
10:10
Authors : Mitsuhiro Terakawa
Affiliations : Keio University; terakawa@elec.keio.ac.jp

Resume : The use of biodegradable polymers as a scaffold in tissue engineering has attracting increasing attention due to its high biocompatibility. Laser processing has advantages in dry processing without using a toxic chemical component and in ease of processing complex-shaped surfaces after molding. Femtosecond laser realized processing of transparent materials including biodegradable polymers via multiphoton absorption with minimized heat affected zone. In this lecture, femtosecond laser processing of poly-L-lactic acid (PLLA) and polylactic-co-glycolic acid (PLGA) as well as the formation of periodic nanostructure on the surface of the polymers will be presented to discuss the potential of laser processing for fabrication of highly-biocompatible scaffold. We demonstrate that high-spatial frequency periodic structure whose periodicity is approximately 100 nm was formed perpendicular to the laser polarization on the surface of PLLA film. The repetition rate of femtosecond laser is considered to be one of the key parameters for the nanostructure formation on a biodegradable polymer. Interestingly, the modification of chemical structure on the laser irradiated area resulted in the changes in the degradability and cell adhesiveness. Experimental results on the laser processing of the biodegradable polymers as well as degradation and cell adhesive properties will be presented.

V.1-.5
11:00
Authors : Fengchi Wu, Xiangxiang Liu, Man Wu, Ye Tian and Zhongwei Niu*
Affiliations : Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China niu@mail.ipc.ac.cn

Resume : Over the past few years, viruses and virus-like particles have received increasing attention in various areas of science, such as vaccinology and nanotechnology. Among these different viruses, tobacco mosaic virus (TMV) has become a popular candidate for nanotechnology research due to its rod-like character. It has been shown that the TMV can be manipulated chemically and genetically, which has resulted in many interesting applications. However, traditional techniques used for the modification of TMV have many drawbacks that limit the usefulness of the TMV to a great extent. Herein, a method for site-specific and high yield modification of tobacco mosaic virus coat protein utilizing genetic code expanding technology and copper free cycloaddition reaction has been established, and biotin-functionalized virus-like particles are built by the self-assembly of the protein monomers. We further demonstrate our recent progresses in the investigation of the cellular internalization mechanism and in vivo biobehavior of these rod-like viral nanoparticles and viral-like particles. 1. Liu, Z.; Qiao, J.; Niu, Z*. Chem. Soc. Rev., 2012, 41, 6178-6194. 2. Wu, M.; Shi, J.; Wang, F.; Niu, Z*. Biomacromolecules, 2013, 14, 4032-4037 3. Wu, F.; Zhang, H.; Wang, J. Y., Niu, Z*. Chem. Commun. 2014, 50, 4007-4009 4. Tian, Y.; Wu, M.; Niu, Z*. Adv. Healthc. Mater. 2014, DOI: 10.1002/adhm.201400508.

V.1-.6
11:45
Authors : Dr. Eric Daniel Głowacki
Affiliations : Linz Institute for Organic Solar Cells (LIOS), Physical Chemistry, Johannes Kepler University, Linz (Austria), Eric_Daniel.Glowacki@iku.at

Resume : We review recent work from our group as well as other researchers demonstrating the efficacy of using natural-origin materials in semiconductor-based devices for interfacing with biology. Many natural materials offer both excellent semiconducting properties, and importantly, electronic as well as ionic conductivity. Biochemical systems are ionic, and not electronic, thus any attempts of active bioelectronics devices must involve ionic/electronic transducing elements. In particular, progress in the use of nanocrystalline and microcrystalline organic hydrogen-bonded pigments will be discussed. These materials have been ubiquitous throughout history and are widely produced today industrially as colorants in everyday products as various as cosmetics and printing inks, and have numerous properties that make them intrinsically biocompatible. The bioconjugation chemistry of these materials and subsequent deployment in electronic devices requiring reliable and specific bio-sensing will be covered.

V.1-.8
12:30
Authors : Jeanne Bernadette TSE SUM BUI
Affiliations : Research Engineer CNRS, jeanne.tse-sum-bui@utc.fr

Resume : Molecularly imprinted polymers are synthetic antibody mimics synthesised by a templating approach at the molecular level, by copolymerisation of functional and crosslinking monomers. Molecular imprinting of small molecules is now considered a routine but the imprinting of proteins still remains a challenge, as the generation of selective imprinted cavities is extremely difficult due to the complexity of their functional sites. In this work, water soluble trypsin and kallikrein molecularly imprinted nanoparticles (MIP-NPs) were obtained by a solid-phase synthesis approach. Glass beads, functionalized with p-aminobenzamidine, an inhibitor of the two enzymes, were used as solid support. This strategy enables an oriented immobilization of the enzyme, upon which thermoresponsive MIP-NPs were synthesized. The MIP-NPs were released by a simple temperature change, resulting in template-free polymers endowed with improved binding site homogeneity, since all the binding sites have the same orientation. Thus, the MIP-NPs exhibit strong affinities (Kdapp : 20-48 pM) for their respective template proteins, comparable to those of natural antibodies. This methodology can be extended to other proteins by immobilising their corresponding substrate, inhibitor or cofactor, thus offering new smart materials for separation and biorecognition.

V.1-.10
14:25
Authors : Emmanuel Stratakis
Affiliations : Foundation for Research and Technology Hellas (FORTH), Institute of Electronic Structure and Laser (IESL), P.O. Box 1527, Heraklion 711 10; stratak@materials.uoc.gr

Resume : Nature offers a diverse wealth of multifunctional and responsive surfaces [1]. We have developed a methodology to realize biomimetic artificial responsive surfaces based on the synergistic effect of direct laser texturing complemented with controlled attachement of specific functional coatings. The laser- textured surfaces comprise microcones exhibiting roughness at both the micro- and the nano- scales, which remarkably mimic the hierarchical morphology and wetting properties of surfaces found in nature. It is shown that depending on the functional coating, pH-, Photo-, Electro-, and Chemo- responsiveness is attained. The implementation of laser engineered surfaces for the development of biomimetic culture substrates for neuron cell adhesion and differentiation is additionally presented. We demonstrate that it is possible to modulate neuron cells’ response and network morphology via laser-assisted tuning of the surface geometrical characteristics. More importantly, it is shown that cellular differentiation can be substantially influenced by the morphological characteristics of the biomimetic substrates. Our results indicate that laser-textured micro/nano Si surfaces provide an excellent platform towards controlling neuronal network topology. The application of our technology for nerve tissue engineering purposes, especially for the treatment of axon degeneration is envisaged. [1] E. Stratakis and V Zorba, Biomimetic Artificial Nanostructured Surfaces, Wiley VCH (2010).

V.1-.12
14:50
Authors : K. Kertész1, G. Piszter1, Zs. Bálint2, L. P. Biró1
Affiliations : 1 Institute of Technical Physics and Materials Science, Centre for Energy Research, 1525 Budapest, PO Box 49, Hungary (http://www.nanotechnology.hu/); 2 Hungarian Natural History Museum, Baross utca 13, H-1088 Budapest, Hungary; kertesz.krisztian@ttk.mta.hu

Resume : In photonic crystals the propagation of electromagnetic waves is forbidden at certain wavelengths, they are reflected. For color generation in the visible, the periodicity of optical properties (refractive index) in the structure has to be in the range of few 100 nm. This condition is well fulfilled by photonic nanoarchitectures of biologic origin, such as the nanocomposites in butterfly wing scales. The building materials are chitin and air. Even minor structural differences could lead to well definite color differences [1]. As we recently discussed one of the possible application of such biological and bioinspired photonic crystal structures is as a selective vapor sensor [2, 3], where crucial aspects are the reproducibility of the measurements and the constant sensor quality. Here we present the comparison of reflectance spectra on the four dorsal wings of a large number of exemplars belonging to the same species. Fast perpendicular nondestructive measurements [4] will be compared with lower throughput but averaging investigations using integrating spheres. It will be pointed out the technical details, also the biological importance of the well determined color. To our knowledge this is the first work investigating the color variation of a butterfly species possessing structural color. [1] Zs. Bálint et al. JRS Interface 73 (2012) 1745 [2] K. Kertesz et al. Appl Surf Sci 281 (2013) 49 [3] G. Piszter et al. Opt Exp 22 (2014) 22649 [4] Zs. Bálint et. al. Genus 21 (2010) 163

V.1-.13
15:30
Authors : R. Gelli, S. Del Buffa, P. Tempesti, M. Bonini, F. Ridi, P. Baglioni
Affiliations : CSGI & Dept. of Chemistry "Ugo Schiff" University of Florence Italy; massimo.bonini@unifi.it

Resume : One of the major topics in bone tissue engineering is the development of synthetic scaffolds in which cells could adhere, proliferate and differentiate, so promoting the physiological biomineralization and the regeneration of the damaged tissue. In this contribution we describe the synthesis and the characterization of a hydrogel based on imogolites and cross-linked gelatin. Imogolites are a class of synthetic clays with hollow fiber shape and nanometric dimensions, exposing two chemically different surfaces, allowing for their asymmetric functionalization and the selective adsorption of molecules and ions. Imogolites were introduced within a gelatin-based matrix to take advantage of its biocompatibility and to impart peculiar mechanical and drug-loading properties. A biocompatible cross-linking agent was adopted in order to further modulate the mechanical properties of the hydrogel. The structural and mechanical properties of the samples were studied by means of different techniques (SAXS, XRD, FT-IR, SEM, TGA, syringe injectability), showing that the material could be either formulated as an injectable fluid, as well as a macroporous scaffold, by properly tuning its composition and by applying cryo-treatments. The macroporous scaffolds was also subjected to a mineralization process, highlighting that imogolite nanotubes do not prevent the growth of hydroxyapatite, but instead promote its crystallinity.

V.1-.15
16:15
Authors : Jianzhi Zhu, Wenjie Sun, Xiangyang Shi*
Affiliations : College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People’s Republic of China. E-mail: xshi@dhu.edu.cn

Resume : Hybrid nanogels are promising nanoscale materials for biomedical imaging applications, since they inherit the superiority of bulk hydrogels as well as characteristic of nanoparticles (NPs) stabilized by hydrophilic polymers [1, 2]. γ-Polyglutamic acid (γ-PGA) has been widely used in different biomedical applications due to its favorable biocompatibility, high aqueous dispersibility, and stable interior network structure [3], however, the use of γ-PGA-based hybrid nanogel systems in magnetic resonance (MR) imaging has not been reported yet. Herein, we developed a facile approach to forming iron oxide NP-loaded γ-PGA hybrid nanogels for T2-weighted MR imaging applications. In this study, γ-PGA with carboxyl groups activated by 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride in aqueous solution was firstly emulsified, followed by in situ chemical crosslinking with polyethyleneimine (PEI)-coated iron oxide NPs (PEI-Fe3O4) with a size of 8.9 ± 2.1 nm synthesized via a mild reduction route. The formed γ-PGA nanogels containing iron oxide NPs (γ-PGA/PEI-Fe3O4) with a size of 150 nm are water-dispersible, colloidally stable, noncytotoxic in a given concentration range, and display a relatively high r2 relaxivity (171.1 mM-1 s-1). Likewise, the hybrid nanogels can be uptaken by cancer cells at a relatively high level. These properties rendered the formed γ-PGA/PEI- Fe3O4 nanogels with an ability to be used as effective contrast agent for MR imaging of cancer cells in vitro and the xenografted tumor model in vivo (Fig. 1) via the passive enhanced permeability and retention effect after intravenous injection. This developed γ-PGA/PEI-Fe3O4 hybrid nanogels may hold great promise to be used as a novel contrast agent for MR imaging or other theranostic applications. Acknowledgements: This research is financially supported by the Sino-German Center for Research Promotion (GZ899) and the National Natural Science Foundation of China (21273032). References [1] Singh S, Möller M, Pich A. Journal of Polymer Science Part A: Polymer Chemistry. 2013;51:3044-57. [2] Gonçalves M, Maciel D, Capelo Db, Xiao S, Sun W, Shi X, et al. Biomacromolecules. 2014;15:492-9. [3] Wang S, Cao X, Shen M, Guo R, Bányai I, Shi X. Colloids and Surfaces B: Biointerfaces. 2012;89:254-64.

V.Y..4
16:25
Authors : Dongyoon Kim1, Subeom Park3, Byung Hee Hong3, Myung-Han Yoon1,2
Affiliations : 1 School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea 2 Department of Nanobio Materials and Electronics (WCU), Gwangju Institute of Science and Technology, Gwangju 500-712, Korea 3 Department of Chemistry, Seoul National University, Seoul 151-747, Republic of Korea; kim@gist.ac.kr

Resume : In this work, we report on the nano-engineered bacterial cellulose via in-situ incorporation of graphene oxide and its application to the artificial neuronal culture scaffold. These materials exhibit excellent cell viability, optical transparency, controlled porocity and favorable mechanical modulus which successfully support for primary neuronal cultures. Furthermore, the resultant three-dimensional neuronal network in this scaffold mimics various phenotypes of neurons in the physiological condition, for example, cellular morphology, axonal outgrowth dynamics and branching and also the layered structure of a real brain tissue. We expect that this nanocarbon-hybridized cellulose can serve as a model 3D neuronal culture useful for the neuroscience research at the well-defined and physiologically-mimicking condition.

V.Y..6
16:30
Authors : Anansa Ahmed and R. V. Ramanujan
Affiliations : School of Materials Science and Engineering, Nanyang Technological University, Singapore-639798; anansa1@e.ntu.edu.sg

Resume : Current research is focusing on creating higher forms of materials, structures and systems by providing them with “life” functions, particularly focusing on incorporating multifunctionality into single materials. Magnet filler-polymer matrix composites (MagPol) are promising materials in which to incorporate multifunctionality because of the flexibility in the choice of polymer matrix and magnetic fillers. The use of magnetic fillers has several advantages which include remote contactless heating and actuation, several actuation modes, high actuation strain and strain rate, self-sensing and quick response. Or work focuses on the development of a tri-functional composite capable of damage sensing, self healing and actuation for use as an artificial actuating skin or muscle. By choosing an appropriate polymer matrix, a sensing element and magnetic nanoparticle filler, we can tune the response of the composite. A commercially available stilbene dye is used to impart a strain sensing functionality to the composite with a visible colour change after plastic deformation. This dye is added to a thermoplastic polymer matrix capable of undergoing thermally activated self healing via a reptation mechanism. The required thermal energy is provided by Curie temperature tuned magnetic nanoparticle fillers so as to prevent pyrolysis of the polymer. Due to the presence of the magnetic filler, the composite also shows actuation under an externally applied magnetic field.

V.Y..7
16:35
Authors : Weihong Jin, Guosong Wu, Hongqing Feng, Ang Gao, Hao Wu, Xiang Peng, Paul K. Chu
Affiliations : Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong; whjin2-c@my.cityu.edu.hk

Resume : Rare-earth magnesium alloys which do not contain harmful aluminum are attractive to biomedical applications such as cardiovascular stents and orthopedic implants. However, the major limitation is the rapid degradation rate in the physiological environment leading to loss of mechanical integrity, excessive production of hydrogen, potential toxicity, and insufficient biocompatibility. Therefore, it is crucial to retard and control the initial corrosion rate of magnesium alloys. In this work, without introducing extraneous elements, a small amount of Nd is introduced into rare earth WE43 Mg alloy by ion implantation to improve the corrosion resistance. The surface composition, morphology, polarization, and electrochemical properties, as well as weight loss, pH, and leached ion concentrations, after immersion are systematically evaluated to determine the corrosion behavior. The cell adhesion and viability are also determined to evaluate the biological response in vitro. A relatively smooth and hydrophobic surface layer composed of mainly Nd2O3 and MgO is produced and degradation of the Nd-implanted WE43 magnesium alloy is significantly retarded. Moreover, significantly enhanced cell adhesion and excellent biocompatibility are observed after Nd ion implantation. The surface treatment provides a practical strategy to enhance the corrosion resistance and biological properties of WE43 Mg alloy simultaneously.

V.Y..8
16:40
Authors : Yi Zhao, Aude Falcimaigne-Cordin, Karsten Haupt
Affiliations : Compiègne University of Technology, CNRS FRE 3580 Enzyme and Cell Engineering Laboratory, Compiègne 60203, France Yi ZHAO (yi.zhao@utc.fr), Aude FALCIMAIGNE CORDIN (aude.cordin@utc.fr), Karsten HAUPT (karsten.haupt@utc.fr)

Resume : Molecularly imprinted polymers (MIPs) are synthetic receptors that possess the similar binding properties for a specific target molecule to biological antibodies. Besides the well-known applications in separation and analytical sciences for the environment and food analysis, MIPs also emerged recently in the biochemical field as diagnostic sensor, chemicals traps to remove undesirable substance from the body, smart materials responsive to the modification of the surrounding environment or as drug delivery system for the controlled release of bio-active substances, where usually the combination of biocompatibility and degradability can be advantageous for their biological applications. Biochemically degradable MIPs have been developed for a potential application as an activation-modulated drug delivery system. Degradable MIPs have been synthesized using cleavable cross-linkers containing, for example, a disulfure bond. In reducing media, as found in intracellular cell, the disulfure bond could be reduced resulting to the cleavage of the polymer chains. In this work, different forms of cleavable MIPs (bulks, nanoparticles, nanogels) were synthesized by different polymerization methods using S-propanolol as model template. The binding properties were examined and compared to conventional non-degradable MIPs. The efficiency of MIP degradation was evaluated with different cleavable agents, monitored by chemical functional modification, molecular weight decrease and morphological change. The in vivo biocompability and intracellular localization were performed as well.

V.Y..9
16:45
Authors : Sofia Nestora (a), Franck Merlier (a), Magali Bartkowski (a), Bin Li (a), Andrew Greaves (b), Karsten Haupt (a)*, Bernadette Tse Sum Bui (a)*
Affiliations : a) Compiègne University of Technology, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France; b) Laboratoires de Recherches de L'OREAL, 1 av Eugène Schueller, 93600 Aulnay sous Bois, France *jeanne.tse-sum-bui@utc.fr, karsten.haupt@utc.fr

Resume : One of the main constituants of human sweat, responsible for axillary malodors, are branched medium-chain (C6-C10) volatile fatty acids such as (E)-3-methyl-2-hexenoic acid (3M2H) and 3-hydroxy-3-methyl-hexanoic acid (3H3MH). They are produced from their non-odorant precursors, the glutamine conjugates, by the action of an N-acylglutamine aminoacylase from Corynebacteria naturally present on the skin surface. In order to prevent the formation of malodor, one possible approach would be to capture and thus render inaccessible these precursors, hence preventing the formation of odorant acids. Molecularly imprinted polymers (MIPs) are synthetic antibody mimics that are produced by a templating process at the molecular level. They are able to specifically recognize and bind their molecular targets, and therefore seem to be ideal candidates for capturing the above precursors. MIPs based on the monomer N-acryloyl-p-aminobenzamidine, which can form strong stoichiometric electrostatic interactions with carboxylates, were synthesized, using as template, structural analogues of the glutamine conjugates. The resulting MIPs showed high binding capacity and specificity for their imprinting templates and the glutamine conjugate precursors of 3M2H and 3H3MH in ‘artificial sweat’, a medium consisting of urea, lactic acid, albumin and sodium chloride. An LC/MS-MS method was then optimized to quantify the glutamine conjugates and their hydrolysis products, the odorant acids 3H3MH and 3M2H, simultaneously in human sweat. The results show that the MIP could capture more of these analytes than the corresponding non-imprinted control polymer (NIP). Thus, these MIPs have great potential for applications as anti-odor agents in cosmetics.

V.Y..10
16:50
Authors : Jingjing Xu, Bin Li, Karsten Haupt and Bernadette Tse Sum Bui*
Affiliations : Compiègne University of Technology, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France; *jeanne.tse-sum-bui@utc.fr

Resume : Salicylic acid (SA) is frequently used as a topical treatment for skin inflammatory disorders like acne, psoriasis and seborrheic dermatitis. The main side effects caused by this treatment are acute irritation and moderate chemical burns. One way to control this problem would be to control the delivery of SA. Sol-gel-based molecularly imprinted polymers (MIPs) for salicylic acid (SA) were synthesized and evaluated in aqueous environments in the aim to apply them as drug delivery carriers. The sol-gel polymers were prepared with 3-(aminopropyl)triethoxysilane and trimethoxyphenylsilane, as functional monomers and tetraethyl orthosilicate as crosslinker. The MIP was very specific as negligible binding was observed for the NIP. The MIP has a very high binding capacity for the drug in ethanol, a solvent compatible with drug formulation and biomedical applications. In vitro release profiles of the polymers in water were then evaluated and the results were modeled by Fick’s law of diffusion and the power law. Analysis shows that the release mechanism was predominantly diffusion-controlled. To the best of our knowledge, this is the first example of a sol-gel MIP that was demonstrated for its potential as drug delivery device for controlled drug release.

V.Y..11
17:00
Authors : Min Hee Kim1, Dong Su Im1, Donghwan Cho2, Oh Hyeong Kwon2, Won Ho Park1,*
Affiliations : 1 Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, Korea 2 Department of Polymer Science and Engineering Kumoh National Institute of Technology, Gumi, Gyeongbuk 730-701, Korea parkwh@cnu.ac.kr

Resume : Tissue engineering has potential to address this need through the combination of biomaterials, growth factors, and cells. Highly porous scaffolds are generally used as the substrate for anchorage dependent cells and to facilitate nutrient and metabolite distribution to guide cell growth leading to new bone tissue formation. Hydroxyapatite (HAP) has been investigated for bone replacement since this material mimics natural bone mineral features. HAP has been studied extensively in cell culture and possesses osteoconductivity. Regenerated Bombyx mori silk fibroin (SF) has excellent biological and mechanical properties, including biocompatibility, programmable biodegradability, and remarkable strength and toughness. One of the important physical forms for biomaterials is the formation of hydrogels, which has been extensively studied for a variety of polymers. The sol-gel transition depended on the concentration of the protein, temperature, and pH. In the SF hydrogel, random coil to β-sheet (physical cross-linking) structural transitions were noted during the process of hydrogelation. Due to the β-sheet formation, SF exhibits relatively slow degradation in vitro and in vivo, compared to collagen and many other biopolymers. In this study, the synthesis and characterization of bone-like mineral HAP into highly porous biodegradable silk fibroin scaffold with via chemical cross-linking reaction of SF by gamma-ray were investigated.

V.Y..13
17:05
Authors : M. Daoud Attieh , A. Elkak, A. Falcimaigne-Cordin, K. Haupt
Affiliations : Compiègne University of Technology CNRS Enzyme and Cell Engineering Laboratory; mira.daoud-attieh@utc.fr

Resume : Molecularly imprinted polymers (MIPs) are tailor-made synthetic receptors that are able to specifically recognize and bind their target molecules. Due to their high chemical and physical stability, easy availability, low cost and high selectivity, MIPs are considered as serious alternatives to natural receptors, such as antibodies. They can thus be widely used in various applications where selective binding is required, such as immunoassays, affinity separation, and biosensors [1,2]. Recently, it has been suggested that MIPs could be applied as drug delivery systems for their controlled release properties [3]. Free radical polymerization is often used to prepare molecular imprinting materials such as hydrogels and typical chemical initiators and/or chemical catalysts are frequently used to initiate the polymerization. However, exposure to these free radical polymerization reagents leads to toxic final compounds, hence limiting their use in biomedical, pharmaceutical and environmental applications [4]. Herein, we describe, for the first time, a new approach for the synthesis of molecularly imprinted hydrogels by free radical polymerization using less toxic conditions. Immobilized initiator systems were developed for nanoparticles synthesis in aqueous media, and applied to the MIP preparation. Different templates such as proteins and small analytes were imprinted. The efficiency of the resulting MIP hydrogels were compared to MIPs prepared by traditional free radical polymerization. References [1] R. Arshady, K. Mosbach, Makromol Chem 1981, 182, 687 – 692. [2] K. Haupt, AV. Linares, M. Bompart, B. Tse Sum Bui, Top Curr Chem 2012, 325, 1-28. [3] R. Suedee, C. Bodhibukkana, N.Tangthong, C. Amnuaikit, S. Kaewnopparat, T. Srichana, J Control Release 2008, 129, 170-178. [4] M. Shirangi, J. Toraño, B. Sellergren, W. Hennik, G. Somsen, C. Nostrum. Bioconjug Chem 2015, 26, 90-100.

V.Y..14
17:15
Authors : E. Sokullu, A. Berchtikou, M.A. Gauthier, T. Ozaki
Affiliations : Institut national de la recherche scientifique, INRS Énergie, Matériaux et Télécommunications, 1650, boul. Lionel-Boulet, Varennes (Québec) J3X 1S2C; E-mail: sokullu@emt.inrs.ca

Resume : The selective generation of plasmonic nanobubbles (PNB) inside cancer cells has recently been proposed as a novel theranostics platform. In general, PNB therapy relies on receptor-mediated endocytosis of gold NPs and formation of gold NP aggregates within the cells. Exposure of the gold aggregates to a short laser pulse transiently superheats them, leading to the formation of a bubble which mechanically disrupts the surrounding tissue. As the ablation of cells can be confirmed due to the high optical scattering of bubbles, PNB can also be tuned for diagnosis purposes to generate sublethal small bubbles for detection of cancer cells. However, variability in the cellular uptake and subsequent aggregation of gold NPs may be detrimental to reproducibility of PNB. In this study bacteriophages are exploited as biological templates for the synthesis of well-defined gold aggregates. M13 and T4 phage display platforms were used for this purpose. Gold NP self-assembly can be directed by displaying molecular recognition moieties on the phage surface, and targeting to cancer cells can be achieved by displaying targeting peptides. The number of functional moieties on the phage surface can be controlled at the genetic level. Moreover, different sizes of gold particles can be conjugated to the phage surfaces, which will enable control over the characteristics of the nanobubble generated at a given laser fluence.

V.Y..16
17:30
Authors : Dongyoon Kim1, Subeom Park3, Byung Hee Hong3, Myung-Han Yoon1,2
Affiliations : 1 School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea 2 Department of Nanobio Materials and Electronics (WCU), Gwangju Institute of Science and Technology, Gwangju 500-712, Korea 3 Department of Chemistry, Seoul National University, Seoul 151-747, Republic of Korea; kim@gist.ac.kr

Resume : In this work, we report on the nano-engineered bacterial cellulose via in-situ incorporation of graphene oxide and its application to the artificial neuronal culture scaffold. These materials exhibit excellent cell viability, optical transparency, controlled porocity and favorable mechanical modulus which successfully support for primary neuronal cultures. Furthermore, the resultant three-dimensional neuronal network in this scaffold mimics various phenotypes of neurons in the physiological condition, for example, cellular morphology, axonal outgrowth dynamics and branching and also the layered structure of a real brain tissue. We expect that this nanocarbon-hybridized cellulose can serve as a model 3D neuronal culture useful for the neuroscience research at the well-defined and physiologically-mimicking condition.

V.V-P1..4
17:30
Authors : Victor Tudorica, Neil R. Thomas
Affiliations : School of Chemistry, Centre for Biomolecular Sciences, University of Nottingham, United Kingdom

Resume : Spider silk is a fibrous material which displays high strength and toughness. Additionally it is thin and biocompatible which gives it a wide variety of biomedical applications. Spiders use it when forming webs in order to aid their movement, catch prey or escape a predator. The diving bell spider (Argyroneta aquatica) is the only spider that spends almost its entire life underwater. In order to fulfil its oxygen needs, it forms a bubble (diving bell). This outstanding spider-silk construct functions both as an air reservoir and as a physical gill allowing the oxygen- carbon dioxide exchange while underwater. The unique feature raises the question of whether the spidroins (spider silk protein blocks) of water spiders are fundamentally different in structure to those of terrestrial spiders. Harnessing the properties of such a material could lead to applications in healthcare and nanotechnology. Recently, SEM images have shown that the “diving bell” silk has a similar structure to that of Polymer Electrolyte Membrane Fuel Cell (PEMFC) gas diffusion layers (GDLs) which combined with its selective gaseous exchange properties makes it a candidate for applications such as dialysis equipment or fuel cell membranes. Testing the material’s suitability for such applications requires a thorough understanding of the silken dumbbell at molecular level (by RNA sequencing and peptide sequencing). Our preliminary results indicate the presence of the highly conserved C-terminus spidroin motif

V.V-P1..5
17:30
Authors : Jianzhi Zhu, Wenjie Sun, Xiangyang Shi
Affiliations : College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People’s Republic of China. E-mail: xshi@dhu.edu.cn

Resume : Hybrid nanogels are promising nanoscale materials for biomedical imaging applications, since they inherit the superiority of bulk hydrogels as well as characteristic of nanoparticles (NPs) stabilized by hydrophilic polymers [1, 2]. γ-Polyglutamic acid (γ-PGA) has been widely used in different biomedical applications due to its favorable biocompatibility, high aqueous dispersibility, and stable interior network structure [3], however, the use of γ-PGA-based hybrid nanogel systems in magnetic resonance (MR) imaging has not been reported yet. Herein, we developed a facile approach to forming iron oxide NP-loaded γ-PGA hybrid nanogels for T2-weighted MR imaging applications. In this study, γ-PGA with carboxyl groups activated by 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride in aqueous solution was firstly emulsified, followed by in situ chemical crosslinking with polyethyleneimine (PEI)-coated iron oxide NPs (PEI-Fe3O4) with a size of 8.9 ± 2.1 nm synthesized via a mild reduction route. The formed γ-PGA nanogels containing iron oxide NPs (γ-PGA/PEI-Fe3O4) with a size of 150 nm are water-dispersible, colloidally stable, noncytotoxic in a given concentration range, and display a relatively high r2 relaxivity (171.1 mM-1 s-1). Likewise, the hybrid nanogels can be uptaken by cancer cells at a relatively high level. These properties rendered the formed γ-PGA/PEI- Fe3O4 nanogels with an ability to be used as effective contrast agent for MR imaging of cancer cells in vitro and the xenografted tumor model in vivo (Fig. 1) via the passive enhanced permeability and retention effect after intravenous injection. This developed γ-PGA/PEI-Fe3O4 hybrid nanogels may hold great promise to be used as a novel contrast agent for MR imaging or other theranostic applications. Acknowledgements: This research is financially supported by the Sino-German Center for Research Promotion (GZ899) and the National Natural Science Foundation of China (21273032). References [1] Singh S, Möller M, Pich A. Journal of Polymer Science Part A: Polymer Chemistry. 2013;51:3044-57. [2] Gonçalves M, Maciel D, Capelo Db, Xiao S, Sun W, Shi X, et al. Biomacromolecules. 2014;15:492-9. [3] Wang S, Cao X, Shen M, Guo R, Bányai I, Shi X. Colloids and Surfaces B: Biointerfaces. 2012;89:254-64.

V.V-P1..6
17:35
Authors : Oleksii Dubok, Iryna Bozhyk, E.Buzaneva (1), D.I.Zabolotny, H.A.Karas(2), N.Kurgan, V.Karbivskiy(3)
Affiliations : (1) Institute for Problem of Material Science NASU and TSN University of Kyiv, Ukraine. (2) O.S. Kolomyichenko Otolaryngology Institute NA Medical Sciences of Ukraine, Kyiv, Ukraine (3) SPM&RS-Centre IMP NASU, Kyiv, Ukraine

Resume : To the purpose of modeling processes after implantation of bioactive synthesized HAp ceramic and to evaluate bioadhesion to cell culture, biofilm growth and cytotoxicity of domestic bioactive ceramic SynthetBone (Lab.synthesis in Dep. Analytical Chemistry and Functional Ceramics, IPMS NASU , Kyiv) on different stages of their interaction with the environment of tissue in vitro studies have been carried out using cell cultures. The conventional method of culturing cells have been used with HEP- 2 cells in DMEM culture medium (Sigma, USA) and with addition of penicillin, streptomycin and 5% fetal calf serum in 24 well plates in an incubator at 37 ° C in atmosphere with 5% СО2.. The replacement of the culture medium performed daily. Such varieties of the SynthetBone have been used: powder of composed nanoparticles of nanocomposite hydroxyapatite (HAp) - tricalcium phosphate (TCP) (80 : 20%) (particle has size 25 - 40 nm, granules - 0.3 -0.5 mm, pore - 50 - 150mkm); bioglass granules and bioglass-ceramics granules. In two parallel experiments the freshly calcite ceramics have been used or the same ceramics that was modified by overnight exposure in helofuzyn. The cells investigation by the microscopy and fluorescent inverted microscope methods carried out. All experiments showed tight adhesion of cells to ceramic and active multilayered cell growth at the surface and near area. It was reveal what the fresh calcite ceramics inhibit of cell growth was observed during the first 1.5 - 2 days compared to the ceramics modified by helofuzyn. Fluorescent study also found a higher level of luminescence of nuclei and cytoplasm of cells near and on the modified ceramics at the first few days of cultivation, reflecting the increase in their proliferate activity. These features can be explained by the active adsorption at the surface of fresh calcite ceramics of components of the culture solution and the excretion of some elements from these surfaces accumulated on them due to segregation processes during calcination. These processes are especially significant during first time after implantation and are accompanying by local changes in pH and ionic composition of the solution, which causes an initial delay in cell growth. Structural and chemical characteristics of HAp surface with cells grown including AFM, XPS, Raman will be presented as confirmation developed models bioactivity for this surface.

V.V-P1..7
17:35
Authors : Ohad Goldbart1, Anastasia Sedova1,2, Lena Yadgarov1, Rita Rosentsveig1, Dmitry Shumalinsky3, Leonid Lobik3, H. Daniel Wagner1 and Reshef Tenne1
Affiliations : 1. Department of Materials and Interfaces, Weizmann Institute, Rehovot 76100, Israel 2. Azrieli - College of Engineering Jerusalem, P.O.Box 3566, Jerusalem, 9103501, Israel 3. Urology Department, Barzilai Medical Center, Hahistadrout St. 2, Ashkelon 78278, Israel

Resume : Insertion of endoscopes and other medical devices to the human body are ubiquitous, especially among aged males. The applied force for the insertion/extraction of the device from the urethra must overcome the endoscope (or catheter)-surface human-tissue interaction. In daily practice a gel is applied on the endoscope surface, in order to facilitate its entry to the urethra, providing also a local anesthesia. In the present work MoS2 nanoparticles, with fullerene-like structure and MoS2 doped with minute amounts of rhenium atoms, have been added to a commercial gel, in order to reduce the metal\catheter-urethra interaction and alleviate the potential damage to the epithelial tissue. In order to compare between the different gels a urethra model was designed and fabricated, which allowed a quantitative assessment of the applied force for extraction of the endoscope from a soft polymer-based ring. It is shown that the Re-doped nanoparticles reduce the traction force used to retrieve the metallic lead of the endoscope or the catheters from the soft ring by a factor close to three times with respect to the original gel. The mechanism of the mitigation of both friction and adhesion forces in these systems by the nanoparticles is discussed.

V.V-P1..8
17:35
Authors : Anansa Ahmed and R. V. Ramanujan
Affiliations : School of Materials Science and Engineering, Nanyang Technological University, Singapore-639798; anansa1@e.ntu.edu.sg

Resume : Current research is focusing on creating higher forms of materials, structures and systems by providing them with “life” functions, particularly focusing on incorporating multifunctionality into single materials. Magnet filler-polymer matrix composites (MagPol) are promising materials in which to incorporate multifunctionality because of the flexibility in the choice of polymer matrix and magnetic fillers. The use of magnetic fillers has several advantages which include remote contactless heating and actuation, several actuation modes, high actuation strain and strain rate, self-sensing and quick response. Or work focuses on the development of a tri-functional composite capable of damage sensing, self healing and actuation for use as an artificial actuating skin or muscle. By choosing an appropriate polymer matrix, a sensing element and magnetic nanoparticle filler, we can tune the response of the composite. A commercially available stilbene dye is used to impart a strain sensing functionality to the composite with a visible colour change after plastic deformation. This dye is added to a thermoplastic polymer matrix capable of undergoing thermally activated self healing via a reptation mechanism. The required thermal energy is provided by Curie temperature tuned magnetic nanoparticle fillers so as to prevent pyrolysis of the polymer. Due to the presence of the magnetic filler, the composite also shows actuation under an externally applied magnetic field.

V.V-P1..11
17:35
Authors : Jingjing Xu, Bin Li, Karsten Haupt and Bernadette Tse Sum Bui*
Affiliations : Compiègne University of Technology, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France; *jeanne.tse-sum-bui@utc.fr

Resume : Salicylic acid (SA) is frequently used as a topical treatment for skin inflammatory disorders like acne, psoriasis and seborrheic dermatitis. The main side effects caused by this treatment are acute irritation and moderate chemical burns. One way to control this problem would be to control the delivery of SA. Sol-gel-based molecularly imprinted polymers (MIPs) for salicylic acid (SA) were synthesized and evaluated in aqueous environments in the aim to apply them as drug delivery carriers. The sol-gel polymers were prepared with 3-(aminopropyl)triethoxysilane and trimethoxyphenylsilane, as functional monomers and tetraethyl orthosilicate as crosslinker. The MIP was very specific as negligible binding was observed for the NIP. The MIP has a very high binding capacity for the drug in ethanol, a solvent compatible with drug formulation and biomedical applications. In vitro release profiles of the polymers in water were then evaluated and the results were modeled by Fick’s law of diffusion and the power law. Analysis shows that the release mechanism was predominantly diffusion-controlled. To the best of our knowledge, this is the first example of a sol-gel MIP that was demonstrated for its potential as drug delivery device for controlled drug release.

V.V-P1..12
17:35
Authors : Weihong Jin (1), Qiwen Wang (2), Guosong Wu (1), Guping Tang (1,2), Paul K. Chu (1)
Affiliations : 1Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong; email: paul.chu@cityu.edu.hk 2Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou, 310028, China

Resume : Gene therapy is promising in treating many hereditary or acquired diseases. It is crucial to develop safe and efficient gene carriers to efficiently deliver the genetic materials to the targeted cells with minimal toxicity. Polyethylenimine (PEI) is a widely used cationic polymer in gene delivery systems, but it is difficult to achieve both good therapeutic effects and low toxicity. It is well known that metallic elements play vital roles in biological systems and activities. In this work, the cationic polymer, β-cyclodextrin-polyethylenimine (PEI-CyD), is fabricated with low molecular weight PEI (600Da) crosslinked by β-cyclodextrin (β-CyD) and then doped with a rare-earth element, neodymium (Nd) by a plasma process to produce a new type of gene vector, Nd@PEI-CyD, for potential cancer therapy. Luciferase expression and EGFP transfection experiments performed in vitro reveal that Nd@PEI-CyD has significantly higher transfection efficiency than lipofectamine 2000 and PEI-CyD. Our results also show that the rare-earth element, Nd, stimulates the energy metabolism of cells to produce more ATP, enhances cell uptake by increasing the caveolin expression, and regulates the cellular pathways by adjusting the calmodulin. Our results suggest a new strategy to prepare highly effective metal-incorporated non-viral gene vectors.

V.V-P1..13
17:35
Authors : Yi Zhao, Aude Falcimaigne-Cordin, Karsten Haupt
Affiliations : Compiègne University of Technology, CNRS FRE 3580 Enzyme and Cell Engineering Laboratory, Compiègne 60203, France Yi ZHAO (yi.zhao@utc.fr), Aude FALCIMAIGNE CORDIN (aude.cordin@utc.fr), Karsten HAUPT (karsten.haupt@utc.fr)

Resume : Molecularly imprinted polymers (MIPs) are synthetic receptors that possess the similar binding properties for a specific target molecule to biological antibodies. Besides the well-known applications in separation and analytical sciences for the environment and food analysis, MIPs also emerged recently in the biochemical field as diagnostic sensor, chemicals traps to remove undesirable substance from the body, smart materials responsive to the modification of the surrounding environment or as drug delivery system for the controlled release of bio-active substances, where usually the combination of biocompatibility and degradability can be advantageous for their biological applications. Biochemically degradable MIPs have been developed for a potential application as an activation-modulated drug delivery system. Degradable MIPs have been synthesized using cleavable cross-linkers containing, for example, a disulfure bond. In reducing media, as found in intracellular cell, the disulfure bond could be reduced resulting to the cleavage of the polymer chains. In this work, different forms of cleavable MIPs (bulks, nanoparticles, nanogels) were synthesized by different polymerization methods using S-propanolol as model template. The binding properties were examined and compared to conventional non-degradable MIPs. The efficiency of MIP degradation was evaluated with different cleavable agents, monitored by chemical functional modification, molecular weight decrease and morphological change. The in vivo biocompability and intracellular localization were performed as well.

V.V-P1..14
17:35
Authors : Valeria Califano (1), Francesco Bloisi (2, 3), Antonio Aronne (4), Giovanni Ausanio (2, 3), Libera Nasti (5), Luciano Vicari (2,3)
Affiliations : (1) Istituto Motori - CNR, Napoli, Italy (2) SPIN - CNR, Napoli, Italy (3) Dipartimento di Fisica, Università di Napoli "Federico II", Napoli, Italy (4) Department of Chemical Engineering, Materials and Industrial Production, University of Naples “Federico II”, Napoli, Italy (5) Accademia Di Belle Arti, Napoli, Italy

Resume : MAPLE (Matrix Assisted Pulsed Laser Evaporation) is a pulsed laser deposition technique similar to PLD. The main difference respect to PLD is the target, formed by a frozen solution of the material to be deposed. This feature allows a softer desorption mechanism since most of the laser radiation is absorbed by the solvent. Lipases are enzymes of biotechnological relevance since they catalyze hydrolysis, esterification and transesterification of long-chain acylglycerides, such as lipids. Essential for their industrial application is their immobilization on a water insoluble support. In this work, MAPLE was used to obtain the deposition/immobilization of lipase from Candida Rugosa (CRL). Films of lipase were already obtained from a ice matrix with a laser wavelength of 1064 nm and showed catalytic activity. However, water is poorly absorbing at that laser wavelength. Furthermore, an important phenomenon of protein self-association was highlighted, that could be responsible for lipase reduced activity. Taking advantage on the possibility of freezing a micro-emulsion without inducing phase separation, here we utilized a target composed by a protein stabilized pentane-in-water micro-emulsion. It is demonstrated that this target composition has a protective effect on the unfolding/aggregation of lipase. To lower the target ablation threshold, a low percentage of the excipient m-DOPA, absorbing the1064 nm radiation, was added.

V.V-P1..16
17:35
Authors : Federico Zen, Thomas Duff, M. Daniela Angione, Ronan J. Cullen, Joana Vasconcelos, James Behan, , Dilushan Jayasundara, Eoin M. Scanlan,* Paula E. Colavita*
Affiliations : School of Chemistry and Centre for Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), University of Dublin Trinity College, College Green, Dublin, Dublin D2, Ireland

Resume : The glycocalyx found in certain cell membranes consists of an ensemble of glycosylated molecules, which direct specific cell-cell interactions and biological recognition events and, furthermore, can inhibit nonspecific undesirable adhesion of other cells and molecules at the cell surface. Mimicking the antifouling properties of the glycocalyx offers a promising strategy to prevent clinical problems associated with nonspecific adsorption of plasma proteins on implants and medical devices, such as thrombosis. This films of saccharides or saccharide decorate molecules have recently emerged as new class of synthetic antifouling coatings. Surprisingly, even small monosaccharide units appear capable of preventing/minimising protein fouling. Here, we report a study of protein adsorption at carbohydrate-modified and bare amorphous carbon surfaces, using a combination of spectroscopic and nanogravimetric methods. The adsorption of three proteins from buffer saline solution was investigated using surfaces coated with four monosaccharides. We show results indicating that the carbohydrate layer significantly reduces surface protein adsorption, particularly in the case of protein concentrations close to physiological values in biologically important fluids (e.g. serum). Surface energy, charge and molecular composition are discussed with the purpose of understanding the implications of our results for the design of carbon surfaces that resist protein fouling in biodevice applications.

V.V-P1..19
17:35
Authors : K. Kertész1, G. Piszter1, Zs. Bálint2, L. P. Biró1
Affiliations : 1 Institute of Technical Physics and Materials Science, Centre for Energy Research, 1525 Budapest, PO Box 49, Hungary (http://www.nanotechnology.hu/); 2 Hungarian Natural History Museum, Baross utca 13, H-1088 Budapest, Hungary

Resume : The photonic crystal type nanoarchitectures occurring in butterfly wings are nanocoposites of chitin and air [1]. The chemical changes of the atmosphere surrounding the wings induce small, but measurable color changes [2]. Moreover these changes are substance specific [3]. The mechanism beyond this sensitivity and selectivity is the capillary condensation of the volatiles in the nanocavities of the photonic nanoarchitecture [4, 5]. We showed recently that when using Lycaenid butterfly wings, the color changes can be best interpreted in their 3D visual space [6, 7]. The general findings regarding the mechanism of sensitivity and selectivity, and the parameters influencing the sensing, like the temperature of the wing sensor and vapor saturation [8] will be discussed. [1] Biró, L. P.; Vigneron, J. P.; Laser Photon. Rev. 2011, 5, 27. [2] Potyrailo, R. A., et al.; Nat. Photonics 2007, 1, 123-128. [3] Biró, L. P.; et al.; In Proc. of SPIE Vol. 2007; Vol. 6593, pp. 659318-1. [4] Kertész, K.; et al.;. Appl. Surf. Sci. 2013, 281, 49-53. [5] Tamáska, I.; et al.; J. Insect Sci. 2013, 13, 87. [6] Bálint, Zs.; et al.; J. R. Soc. Interface 2012, 9, 1745-1756. [7] Piszter, G.; et al.; Opt. Express 2014, 22, 22649. [8] Kertész, K.; et al.; Mater. Sci. Eng. C 2014, 39, 221-226.

V.V-P1..21
17:35
Authors : Joana Vasconcelos, M. Daniela Angione, Federico Zen, Ronan J. Cullen, James Behan, Paula E. Colavita*
Affiliations : School of Chemistry and Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Trinity College Dublin, College Green, Dublin D2, Ireland

Resume : Lipids are key components in biological systems, acting as a structural element of cells and being responsible for signalling and storing energy. As surface active compounds, lipids can self-assemble at solid surfaces as intact vesicles or lipid sheets. Understanding how lipids interact with materials and what is their role in determining the physiological response to a biomaterial will contribute to our ability to prevent undesirable bioresponses. Carbon materials, nanomaterials and coatings are currently under intense study for biomaterial and biodevice applications; here we report a study on the interactions between model lipid assemblies and carbon surfaces. The adsorption of phosphatidylcholine (PC) / phosphatidylserine (PS) liposomes onto amorphous carbon surfaces was investigated as a function of pH. We used Infrared Reflectance Spectroscopy (IRRAS) measurements to determine whether PC/PS liposomes adsorb at carbons with different surface chemistry and surface free energy. We also used fluorescence imaging and fluorescence recovery after photobleaching (FRAP) methods in order to understand the mode of adsorption, in particular whether liposomes undergo rupture upon adsorption. Our results suggest that surface modification of carbon can be used to tune the mode of adsorption, a result that has implications for applications of carbon materials and nanomaterials in biology.

V.V-P1..22
17:35
Authors : F. Bertorelle, M. Pinto, G. Fracasso, V. Amendola, M. Colombatti, M. Meneghetti
Affiliations : University of Padova, Department of Chemical Sciences, Nanostructures & Optics Laboratory, fabrizio.bertorelle@gmail.com

Resume : Designing and synthesis of multifunctional nanoparticles for biological application is an actual challenge in which many research groups are now involved. Most of these studies are focused on the lowering the detection limit of cell expressions with respect to current diagnosis techniques. Our results start from the synthesis of naked AuNPs and FeOxNPs obtained with Laser Ablation synthesis in water. Nanoparticles are then combined in a core-shell-satellite type structure using MPTMS. SERS properties of these nanostructures are obtained with a dye embedded in the MPTMS shell. To perform immunomagnetic sorting, satellite AuNP are functionalized with an antibody for membrane prostatic antigen (PSMA). Characterization of nanosystems was performed with UV-Vis-NIR and SERS spectroscopy. Size and shape were determined either by TEM and DLS measurements. Z-potential and EDX measurements were also performed to have the whole comprehension of the physical-chemistry and composition of these nanosystems. Antibody-functionalized core-shell-satellite nanoparticles were incubated with PSMA+ or PSMA- cell lines to demonstrate the possibility to perform selective immunomagnetic sorting of cancer cells. We will also show that combining immunomagnetic sorting with Raman-SERRS measurements performed at single cell level could serve as powerful tool to select and recognize cells with specific antigen expressions.

V.V-P1..23
17:40
Authors : E. Sokullu, A. Berchtikou, M.A. Gauthier, T. Ozaki
Affiliations : Institut national de la recherche scientifique, INRS Énergie, Matériaux et Télécommunications, 1650, boul. Lionel-Boulet, Varennes (Québec) J3X 1S2C; E-mail: sokullu@emt.inrs.ca

Resume : The selective generation of plasmonic nanobubbles (PNB) inside cancer cells has recently been proposed as a novel theranostics platform. In general, PNB therapy relies on receptor-mediated endocytosis of gold NPs and formation of gold NP aggregates within the cells. Exposure of the gold aggregates to a short laser pulse transiently superheats them, leading to the formation of a bubble which mechanically disrupts the surrounding tissue. As the ablation of cells can be confirmed due to the high optical scattering of bubbles, PNB can also be tuned for diagnosis purposes to generate sublethal small bubbles for detection of cancer cells. However, variability in the cellular uptake and subsequent aggregation of gold NPs may be detrimental to reproducibility of PNB. In this study bacteriophages are exploited as biological templates for the synthesis of well-defined gold aggregates. M13 and T4 phage display platforms were used for this purpose. Gold NP self-assembly can be directed by displaying molecular recognition moieties on the phage surface, and targeting to cancer cells can be achieved by displaying targeting peptides. The number of functional moieties on the phage surface can be controlled at the genetic level. Moreover, different sizes of gold particles can be conjugated to the phage surfaces, which will enable control over the characteristics of the nanobubble generated at a given laser fluence.

V.V-P1..25
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Special Session “Biological and Biomimetic surfaces, interfaces on molecular – level organization and characterization” : Invited Chairs: Prof. Insung S.Choi, KAIST, Korea ischoi@kaist.ac.kr and Dr. Emmanuel Stratakis, IESL, FORTH, Greece; stratak@iesl.forth.gr, Dr. Chiung-Wen Kuo, ResearchCenter for Applied Sciences, Academia Sinica, Taiwan, kuo55@gate.sinica.edu.tw, Assisted by Dr. Evi Kavatzikidou, Institute of Electronic Structure and Lasers (IESL), Greece
08:30
Authors : Insung S. Choi
Affiliations : Center for Cell-Encapsulation Research, Molecular-Level Interface Research Center, Department of Chemistry, Department of Bio and Brain Engineering, KAIST, Daejeon 305-701, Korea; ischoi@kaist.ac.kr

Resume : Topography, the physical characteristics of an environment, is one of the most prominent stimuli neurons can encounter in the body. Many aspects of neurons and neuronal behavior are affected by the size, shape, and pattern of the physical features of the environment. A recent increase in the use of nanometric topographies, due to improved fabrication techniques, has resulted in new findings on neuronal behavior and development. Factors such as neuron adhesion, neurite alignment, and even the rate of neurite formation have all been highlighted through nanotopographies as complex phenomena that are driven by intricate intracellular mechanisms. The translation of physical cues is a biologically complex process thought to begin with recognition by membrane receptors as well as physical, cell-to-surface interactions, but the internal biological pathways that follow are still unclear. In this respect, nanotopography would be a more suitable platform on which to study receptor interfaces than microtopography because of the subcellular topographical features that are relevant in scale to the receptor activity. Ultimately, the characterization of this unknown network of pathways will unveil many aspects of the behavior and intracellular processes of neurons, and play an important role in the manipulation of neuronal development for applications in neural circuits, neuroregenerative medicine and prostheses, and much more.

V.2-.1
09:00
Authors : Chiung-Wen Kuo, and Peilin Chen
Affiliations : Research Center for Applied Sciences, Academia Sinica, 128, Section 2, Academia Road, Nankang, Taipei 115, Taiwan; peilin@gate.sinica.edu.tw

Resume : Cells explore the surfaces of materials through membrane-bound receptors, such as the integrins, and use them to interact with extracellular matrix molecules adsorbed on the substrate surfaces, resulting in the formation of focal adhesions. With recent advances in nanotechnology, biosensors and bioelectronics are being fabricated with ever decreasing feature sizes. The performances of these devices depend on how cells interact with nanostructures on the device surfaces. However, the behavior of cells on nanostructures is not yet fully understood. Here we present a systematic study of cell-nanostructure interaction using polymeric nanopillars with various diameters. We also report the development of a well-defined stem cell culture system using surface functionalized polymeric nanopillars with tunable surface chemical and physical properties. Nanopillars, modified with peptides derived from vitronectin, can be used as defined substrates to maintain both human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells for up to 3 months without differentiation. The surface chemical properties of the nanopillars were more critical than the physical properties in maintaining stem cells. However, the physical properties of the nanopillars influenced the stem cell fate. Softer nanopillars promoted the undifferentiated state, and more rigid nanopillars encouraged stem cell differentiation. Such nanopillar system also allows us to selectively sorting specific cells n

V.2-.2
09:50
Authors : Dorota Bartczak, (1) Otto L. Muskens,(1)Tilman Sanchez-Elsner,(2) Timothy M. Millar(2) Antonios G. Kanaras,(1)*
Affiliations : (1) Institute for Life Sciences, Faculty of Physical and Applied Sciences, University of Southampton, UK, SO171BJ (2) Faculty of Medicine, University of Southampton, UK; antoniskan@gmail.com

Resume : In this presentation, we will discuss our recent developments on the use of functional nanoparitcles in biomedical applications. We will mainly focus on the interactions of colloidal gold nanoparticles and endothelial cells. Endothelial cells are the major players in a vital biological process-the angiogenesis. Angiogenesis is the growth of new capillary vessels to bring oxygen and nutrients at the most distant areas of the body. Manipulation of this process could be the key to the development of several diseases including cancer. We will show our recent efforts towards the manipulation of angiogenesis using bio-functionalized nanoparticles.

V.2-.4
10:05
Authors : Yoshikatsu Akiyama
Affiliations : Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University; yakiyama@abmes.twmu.ac.jp

Resume : Temperature-responsive cell culture surfaces, which is poly(N-isopropylacrylamide) (PIPAAm) modified tissue-culture polystyrene (TCPS) (PIPAAm-TCPS), has been used for the fabrication of various types of single monolayer cells sheet such as epithelium keratinocyte, corneal epithelial cell, oral mucosal epithelial cell, urothelial cell, periodontal ligament, cardiomyocyte, hepatocyte cell sheets, and so on. Various types of cell sheets have been directly transplanted to reconstruct tissue, and some of them are clinically applied. PIPAAm-TCPS has been conventionally prepared by using electron beam (EB) irradiation and is currently released as commercially available products. Although PIPAAm-TCPS has been elaborately characterized with FT-IR, XPS, AFM and cell detachment / attachment assay, the interaction between cells and PIPAAm-TCPS surface was not quantitatively estimated. To quantitatively estimate the interaction, we have newly prepared PIPAAm-TCPS having five parallel micro-channels for cell culture by using micro-fabrication technology. This construction allows concurrently generating five different shear forces to apply to cells in individual microchannels having various resistance of each channel and simultaneously gives an identical cell incubation condition to all test channels. Shear stress-dependent cell detachment process from PIPAAm-TCPS was evaluated at various shear stresses.  EB irradiation method allows for mass-production of conventional PIPAAm-TCPS as a commercially-available product. However, the EB irradiation method is costly for the preparation of PIPAAm-TCPS. A facile method for the mass-production has been alternatively required to reduce the cost of mass-production without expensive and special EB irradiation system. In this presentation, we will show microfluidic-based analytical system for evaluating the interaction between cell and PIAPAm-TCPS surface and a facile method for the preparation of PIPAAm-TCPS.

V.2-.5
11:30
Authors : Mingjie Liu
Affiliations : Institute of Chemistry, Chinese Acadamy of Sciences liumj@iccas.ac.cn

Resume : Machine technology frequently puts magnetic or electrostatic repulsive forces to practical use, as in maglev trains, vehicle suspensions or non-contact bearings. In contrast, materials design overwhelmingly focuses on attractive interactions, such as in the many advanced polymer-based composites, where inorganic fillers interact with a polymer matrix to improve mechanical properties. However, articular cartilage strikingly illustrates how electrostatic repulsion can be harnessed to achieve unparalleled functional efficiency: it permits virtually frictionless mechanical motion within joints, even under high compression. Here we describe a composite hydrogel with anisotropic mechanical properties dominated by electrostatic repulsion between negatively charged unilamellar titanate nanosheets embedded within it. Crucial to the behaviour of this hydrogel is the serendipitous discovery of cofacial nanosheet alignment in aqueous colloidal dispersions subjected to a strong magnetic field, which maximizes electrostatic repulsion and thereby induces a quasi-crystalline structural ordering over macroscopic length scales and with uniformly large face-to-face nanosheet separation. We fix this transiently induced structural order by transforming the dispersion into a hydrogel using light-triggered in situ vinyl polymerization. The resultant hydrogel, containing charged inorganic structures that align cofacially in a magnetic flux, deforms easily under shear forces applied parallel to the embedded nanosheets yet resists compressive forces applied orthogonally. We anticipate that the concept of embedding anisotropic repulsive electrostatics within a composite material, inspired by articular cartilage, will open up new possibilities for developing soft materials with unusual functions.

V.2-.7
14:55
Authors : Osamu Suzuki; Takahisa Anada
Affiliations : Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Japan

Resume : Various calcium phosphate ceramics have been examined as scaffold materials for osteoblastic cells in bone tissue engineering. Octacalcium phosphate crystals, if synthesized in a specified condition, enhance mouse bone marrow derived stromal ST-2 cells inoculated on the surface, through increasing phosphorylation of p38 in the eosteoblastic signaling [1]. The change of inorganic ion composition around the crystals, caused by the conversion from OCP to hydroxyapatite (HA) crystals, could be one of factors involved in stimulating the osteoblastic cellular activity [2]. The physicochemical process during the material maturation into the thermodynamically stable phase (HA) could induce the surface morphological changes at nano-meter scale on the OCP material [3]. When OCP is mechanically mixed with amorphous calcium phosphate (ACP), which is a calcium phosphate salt soluble more than OCP, the conversion rate into HA from OCP increases with the modification of surface morphology of OCP [3]. Furthermore, the osteoconductivity of the mixture increases compared to the respective single phase if examined by its implantation into critical-sized rat calvaria defect [3]. Thus, it seems likely that calcium phosphate materials show the bioactivity through physicochemical process under physiological environment. [1] Nishikawa R et al. Dent Mater J 33:242 (2014); [2] Suzuki O et al. Biomaterials 27:2671 (2006); [3] Kobayashi K et al. ACS Appl Mater Interfaces 6:22602 (2014).

V.3-.3
15:20
Authors : Jun Kobayashi, Yoshinori Arisaka, Kazuo Ohashi, Kohei Tatsumi, Kyungsook Kim, Yoshikatsu Akiyama, Masayuki Yamato, and Teruo Okano
Affiliations : Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawadacho, Shinjuku-ku, Tokyo 162-8666, Japan.

Resume : Hepatocyte sheet-based tissue engineering is an attractive method for the treatment of liver diseases. However, hepatocytes rapidly lose their viability and phenotypic functions on isolation from the native in vivo microenvironment of the liver. In this paper, heparin-functionalized poly(N-isopropylacrylamide) (PIPAAm)-grafted cell culture surface, which interacts with heparin-binding proteins such as heparin-binding EGF-like growth factor (HB-EGF), has been designed for maintaining hepatic functions during the cultivation. Heparin-functionalized thermoresponsive cell-culture surface induced the multivalent affinity binding of heparin-binding proteins in active form in the state of shrunken PIPAAm chains at 37 °C. By lowering temperature to 20 °C, the affinity binding between heparin-binding proteins and immobilized heparin was reduced with increasing the mobility of heparin and the swollen PIPAAm chains. When the medium contained less than 10 ng/cm2 of soluble HB-EGF, the hepatocytes were not able to adhere and form their cell sheets. Hepatocytes adhered well and formed their sheets on HB-EGF-bound heparin-functionalized thermoresponsive surface. The secretion of albumin was maintained and higher compared to that on PIPAAm-grafted surfaces with soluble HB-EGF. By lowering temperature to 20 °C, the cultured cell sheets were detached from the surface. The function of hepatic cell sheet was maintained by using advanced thermoresponsive cell culture surfaces.

V.3-.4
 
Invited Special Poster Session : “Hyper Bio Assembler for 3D Cellular System Innovation” with Invited Poster Presentations of Lecturers, Keynote Presenters of May 11-15 sessions for "Face-to-Face" panel discussions. Invited Chairs: Assis. Prof. Yoshikatsu Akiyama, Tokyo Women's Medical University, Japan and Dr. Emmanuel Stratakis, IESL, FORTH, University of Crete, Greece
17:00
Authors : Prof. Dr. Tatsuo Arai
Affiliations : Department of Systems Innovation, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan arai@sys.es.osaka-u.ac.jp, http://bio-asm.jp/english

Resume : Research in a new field, Hyper Bio Assembler for 3D Cellular Innovation (Bio Assembler) started in July 2011 as a five year project supported by Grants-in-Aid for Scientific Research on Innovative Areas from Japanese Ministry of Education (MEXT). The aim of project is to build 3D cellular systems capable of functioning in vitro and to propose an entirely new interdisciplinary area never previously explored. The Bio Assembler will elucidate the principles of ultrahigh speed measurement as well as the manipulation techniques and tissue function expression. The project consists of three research groups; first, ?Measurement and control of cell characteristics? which focuses on measuring physical properties of cells taken from living organisms at high speed and separating target cells useful in constructing cellular systems; second, ?3D cellular system assembly? which aims at shaping and assembling 3D cellular systems with complex morphologies and vascular networks inside; third, ?Analysis and evaluation of 3D cellular systems? which provides analysis and evaluation of growth, differentiation-inducing, morphogenesis controls and transplantation responses of created 3D cellular systems, and conducts functional elucidation as well as comparison with and verification of in vivo with looking for ways to bring our outcomes into the tissue life science such as regenerative medicine, cell assay, and so on. Our final goal is to establish three major academic achievements; ?Cell Sort Engineering?, ?3D Cellular System Design Logic? and ?Cell Sociology? as well as providing innovative measurement and control technologies based on micro/nano robotics.

V.V-P2..1
17:00
Authors : Takahisa Anada, Osamu Suzuki
Affiliations : Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Japan

Resume : A spherical multicellular aggregate can be used as an in vitro model for the 3D cellular construct in tissue engineering and tumors. Oxygen is one of the most important factors for cell survival and growth in three-dimensional culture. The increase in size of spheroid results in a hypoxia and causes cell necrosis in the core of large spheroids. We have developed an oxygen-permeable spheroid culture device using polydimethylsiloxane (PDMS) to prevent oxygen deprivation of the spheroid. In this study, we investigated whether this chip has advantages in the cell proliferation, viability, cellular function, and differentiation in comparison with the conventional non-oxygen-permeable chip. We found that the oxygen-permeable chip is useful for engineering 3D cellular constructs with high viability and functionality for tissue engineering.

V.V-P2..2
17:00
Authors : Yoshikatsu Akiyama
Affiliations : Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, Japan

Resume : Temperature-responsive cell culture surface (TRCS), poly(N-isopropylacrylamide) (PIPAAm) grafted tissue culture polystyrene (TCPS) (PIPAAm-TCPS) prepared by electron beam (EB) irradiation, has been used to fabricate various cells sheets. The fabricated cell sheets has been successfully transplanted to treat the damaged human tissues such as corneal, esophageal ulcerations, periodontitis and so on. Therefore, a lot of researchers are desiring for TRCS to research cell-sheet based tissue regeneration and/or to readily fabricate various tissues used for diagnosis. However, researchers in average laboratory can not prepare the TRCS because EB irradiation system is much expensive and not common equipment. Facile methods for preparing TRCS without EB system have been required. Based on the requirement, we newly developed a facile method for perpetrating TRCS without EB equipment. Advantage of the facile method is that commercially available polystyrene (PSt) surfaces is readily modified with thioxanthone based photo-initiator. In addition, PIPAAm was grafted on the photo-initiator modified PS surface by using visible light irradiation. These procedures were performed by commonly used chemical reaction and equipment. Researchers in the average laboratory also can readily prepare TRCS by this facile method as well as prepare polyacrylamide gel for the electrophoresis.

V.V-P2..3
17:05
Authors : Yukiko T. Matsunaga
Affiliations : The University of Tokyo

Resume : Microvasculatures are greatly involved in the disease such as inflammation, angiogenesis and cancer metastasis, and these events are highly complex, thus sophisticated model is required to understand these mechanism actions at cellular and tissue levels. Recent microtechnologies allow fabrication of three-dimensional (3D) microtissue structures and have a higher capability to control their microenvironments precisely. Since microvasculature is multicellular tissues and also microvascular disease is highly related to the chemical and mechanical factors such as extracellular matrices, shear stress and pressure, in vitro models enable analyzing various interactions from simple to complex state will be useful. Here, we introduce our 3D microvasculature models fabricated by a micro-molding method. Briefly, we prepared collagen microchannels by using collagen gels and PDMS hosting chamber including a needle (120 µm in diameter) as a channel mold. By using the device, continuous lumen structure of human umbilical vascular endothelial cells (HUVECs) surrounded by collagen gels can be formed. Our vasculature model allows continuous monitoring of the vascular permeability and sprouting responding to the angiogenic factors. Compared to the traditional 2D culture system, our model is very useful for the investigation of the vascular disease at both cellular and tissue levels.

V.V-P2..5
17:05
Authors : Masumi Yamada, Minoru Seki
Affiliations : Chiba University, Japan

Resume : Microfluidic technologies are capable of producing functional, micrometer-sized materials including particles, fibers, and sheets, composed of multiple small compartments with different physicochemical properties. In this presentation we briefly introduce our recent achievements on the preparation of anisotropic hydrogel materials and their applications to the construction of in vivo tissue-mimicking microenvironments. Hydrogel microfibers having anisotropic cross sections were prepared by utilizing the stable laminar flow in microfluidic channels, which were employed for tissue engineering applications including the guidance of cell proliferation, formation of heterotypic hepatic micro-organoids, and analysis of cancer cell migration in 3D environments. In addition, unique hydrogel micro-materials were produced such as stripe-patterned heterogeneous hydrogel sheets, yarn-ball-shaped hydrogel beads, and single micrometer-sized ECM beads. These results clearly demonstrated the usefulness of microfluidic technologies for preparing micrometer-sized, cell-encapsulating and/or cell-adhesive hydrogels.

V.V-P2..6
17:10
Authors : Bo Zhu
Affiliations : State Key Lab for Modification of Chemical Fibers and Polymer Materials & College of Materials Science and Engineering, Donghua University, Shanghai,China

Resume : Electrically conducting polymers (ECPs) were widely used to electrically couple with cells in implanted electronic devices and biosensors, mainly thanks to the long-term stability of their electrical properties in biological environments and their mechanical properties comparable with that of the extracellular matrix. To erase/diminish nonspecific interaction for ECPs would not only increase the selectivity of biosensors but also reduce the inflammation risk for implants. In this presentation, we show that with finely tuning the chemical structure, ECPs could specifically interact with cells on a protein-resistance background. Our recent works further demonstrated that with combining specific interaction with protein resistance on ECPs, they could differentiate cells, dynamically interact with and release cells, or even help to construct a three-dimensional platform to capture CTC cells with both high efficiency and selectivity.

V.V-P2..8
17:10
Authors : Paola Palmero1, Helen Reveron2, Laura Montanaro1 and Jérôme Chevalier2,3
Affiliations : 1Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi, 24, 10129 Torino, Italy; email: paola.palmero@polito.it 2Université de Lyon, INSA de Lyon, MATEIS CNRS UMR5510, 20 Avenue Albert Einstein, F-69621 Villeurbanne Cedex, France 3Institut Universitaire de France, 103 bd Saint-Michel, Paris 75005, France

Resume : LONGLIFE (“Advanced multifunctional zirconia ceramics for long-lasting implants”) coordinated by Prof. Jérôme Chevalier from INSA of Lyon, is a Collaborative European project in the frame of the 7th Framework Program. LONGLIFE aims at developing new zirconia-based oral and spine (lumbar inter-vertebral disc) implants, characterized by lifetime longer than 60 years and superior reliability as compared to previous biomedical devices. In order to reach this ambitious goal, efforts should be dedicated to improve the in-vivo stability of zirconia, by one side, and enhance the osseointegration capabilities of the implants in contact with bone, on the other. At the same time, the project aims at developing new ceramic-oriented designs for the implants as well as new multi-physic accelerated testing in vitro, able to reproduce more effectively the different degradation mechanisms and their interplay. In this work, the achievements concerning the design and development of new zirconia-based materials are presented, reached thanks to the synergies between Politecnico of Torino and INSA of Lyon. Among the technical oxide ceramics able to be used as structural biomaterials, zirconia-based ceramics are considered the best choice thanks to their excellent mechanical properties, biocompatibility and aesthetics. However, the current zirconia-based implants suffer from degradation under hydrothermal conditions (low temperature and presence of water), leading to surface degradation and possibly to fracture. Therefore, in order to increase the implants reliability, a strong effort is required to improve the zirconia stability in the presence of water, without decreasing its toughness and strength. The strategy chosen in LONGLIFE towards this goal is reported. Besides an innovative design of the composite material, a controlled synthesis process was used to produce multi-phase composites, having controlled architecture and composition. The results achieved demonstrate the effectiveness of this strategy towards the development of strong, tough and stable materials, fulfilling the objectives of the project.

V.V-P2..10
17:10
Authors : Thomas J. Webster
Affiliations : Department of Chemical Engineering, Northeastern University, Boston, MA 02115

Resume : This presentation will focus on the unprecedented impact nanotechnology has had across all of medicine, specifically focusing on improved disease prevention, diagnosis, and treatment. Disease systems will cover cancer, infection, inflammation, and poor tissue growth including bone, vascular, cardiovascular, bladder, nervous system and others. A particular focus will be placed on sensors that personalize medicine towards one's immune system. Toxicity will also be covered. A new field, picomedicine, will also be introduced concerning how further advances in medicine can be made. Here, the focus will be placed on controlling electron distributions (as opposed to atomic distribution in nanotechnology) to improve disease prevention, diagnosis, and treatment.

V.V-P2..11
17:10
Authors : A. Vlandas, M. Danoy, I. Kurylo, J.C. Galas, A. Estevez-torres, A. Padirac, Y. Rondelez
Affiliations : IEMN/CNRS;IEMN/CNRS;IEMN/CNRS;LPN/CNRS;LPN/CNRS;LIMMS/CNRS and IIS, Univ. Tokyo;LIMMS/CNRS and IIS, Univ. Tokyo;

Resume : Molecular programming (also known as DNA computing) in its various implementations make it possible to implement complex instructions at the molecular scale. One of the DNA framework for molecular programming, the PEN framework developed by Y. Rondelez et al., provides a robust basis on which one can develop DNA based dynamic systems. It relies on a combination of three enzymes (a Polymerase, an Exonuclease and a Nickase) working on 22bp long DNA “templates” which encode relationships between 11bp strands. However the current communication paradigm is grounded in a classical molecular biology approach: inputting information with pipettes and reading states using fluorescence in a real time PCR machine. Such an approach is hard to miniaturize and parallelize and thus limits what can be done with this DNA framework. We report here our work done to use active surfaces for DNA computing. In fact, by working in microfluidics it is possible to benefit from an advantageous surface/volume ratio. This in turn makes it possible to design active surfaces which can release or expose sufficiently large quantity of DNA strand to effectively action our DNA dynamical system. We design these systems to be electrically driven to facilitate interfacing with classical computers. Not only do they provide greater control in giving instruction to DNA computers but they enable important new advances such as spatialisation of input.

V.V-P2..12
17:10
Authors : Heorhii VOROBETS
Affiliations : Yuriy Fedkovych Chernivtsi National University

Resume : The principles of self-organization of intelligent systems - is the basis of the designing of modern computers. The activation of hardware of any computer system implementation by running the bios-program and scan program environment of the operating system. This process has certain analogies with beginning of viability of living organisms from the genetic code of DNA, if we assume that the process bios - activation of computer is accompanied by searching conformity and agreement between a hardware and software system available and the formation of fixed relationships between them. This is especially true for modern branched and network systems, hot plug and auto identification of additional modules and peripherals. Systems that use elements of artificial intelligence and genetic algorithms are usually targeted at solving applied problems and can not influence on the architectural, structural and logical connections within the system. However, the range of tasks that need to be addressed when creating a modern bio integrated and cyber physical systems requires a multifunctional computer tools. On the other hand, the achievement of micro- and nanoelectronics, miniaturization units and of limited applicability in such systems, traditional architecture and system solutions. In this study substantiate the structural and functional and algorithmic methods and approaches to address these problems through the use of adaptive and self reconfigurable computer systems. The essence of adaptive re configuration in this sense is to use the principle of adapting the system to the new conditions of its operation, which is similar to the adaptation and variation in nature. The level of functional variability of the system shall be determined by the results of the knowledge environment functioning and formation of appropriate databases and knowledge. Such problems are currently solved by computer artificial neural network by learning and recognition of signals and images. The principle of self reconfiguration system provides in this case the possibility of correction and fixation of acquired knowledge and its application not only to improve the algorithm of the system, but also the partial modification of its architectural design. This is equivalent to the genetic modification of the DNA code of living organisms. Our results show that the proposed approach and the structural and algorithmic solutions can be the basis for the synthesis of multifunctional intelligent computer systems embedded in kiber physical devices and systems for various levels and purposes.

V.V-P2..13
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Invited Special ONE DAY Session: “Biogenic, Bio - mimetic minerals, - mimetic supramolecular materials and - activated metal, metal hybrid surfaces for bone engineering” : Dr Paola Palmero, Vice Chair of the CostAction MP1301, Department of Applied Science and Technology, Politecnico di Torino, paola.palmero@polito.it; Dr. Maria Farsari , IESL,FORTH, Greece, mfarsari@iesl.forth.gr; and Dr. Donata Iandolo, Linkoping University, Sweden, donata.iandolo@liu.se; Assisted by MS, Researcher Oleksii Dubok, TSN University of Kyiv, IPMS NASU, Ukraine, oleksii.dubok@gmail.com
08:45
Authors : Paola Palmero
Affiliations : Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi, 24, 10129 Torino, Italy; email: paola.palmero@polito.it

Resume : The dramatic increase in average life expectancy during the 20th century ranks as one of society’s greatest achievements. At the same time, an increased quality of life is expected as well. As a result, there is currently an ever increasing demand for biomedical devices with a perfect reliability and lifetime longer than 60 years. For this reason, new customized implants need to be developed, thus stimulating the research towards new materials, tailored macro- and micro-structures and advanced manufacturing methods. Commercial calcium phosphates are restricted to applications that require only moderate load bearing abilities. On the other hand, high toughness bioinert materials (such as alumina/zirconia composites) are used in the articulating couple of artificial joint implants, but they do not support direct bone/material interfaces or bond. To date, there is no tough and strong ceramic in regular clinical use with ability to create a strong, biologically relevant, interface with bone. New customized techniques, such as additive manufacturing, are currently under development leading to tailored material architecture and customized biomedical devices. However, most of the advances are currently achieved at the laboratory scale. The industrial development can be reached only if the gap between academic research, clinical use and commercial production is reduced, highlighting the need of continuous exchanges and cooperation between surgeons, industrials, engineers, material scientists and biologists. For this reason, this Cost Action, coordinated by Dr. Francis Cambier from Belgium Ceramic Research Centre of Mons, aims at creating the seed for the European research and industry collaboration, combining basic knowledge from academic laboratories, R&D centres, medical units from hospitals, and a significant number of companies. Currently, Institutions from 29 European Countries are involved in the Action, counting also on the participation of extra-Europe participants such as USA and Canada.

V.4..1
09:00
Authors : Anthi Ranella, Alexandros Selimis, Maria Farsari
Affiliations : IESL-FORTH, N. Plastira 100, 70013, Heraklion, Greece

Resume : Over the recent years, a highly interdisciplinary field lying on the interface of Biology, Chemistry and Engineering has been developed concerning the design and the fabrication of bioinspired surfaces for the development of functional substitutes for damaged tissue. Towards this aim, the Direct Laser Writing (DLW) technique based on Multi-Photon Polymerization (MPP) of photosensitive materials has established a strong foothold, since it enables the fabrication of three-dimensional (3D) structures with sub-micron resolution. A decisive parameter for the accurate structures fabrication by DLW, besides the irradiation ones, is the structuring material choice. Indeed, it is the material properties that ineluctably and crucially determine definitely the success of the printing process and additionally the resolution of the laser fabricated structures as well as the potentiality of their bulk and surface functionalization. Here, we present the variety of the employed materials extended from non-biodegradable hybrid organic-inorganic ones to biodegradable synthetic or natural biopolymers. Other than that, the effect of the 3D structures topography on different cell responses are discussed. In particular, cell responses such as proliferation, migration and differentiation are investigated.

V.4..2
09:30
Authors : Donata Iandolo*, Lim Jing?, Akhilandeshwari Ravichandran?, Jerry Chan#, Teoh Swee-Hin?, Daniel Simon*, Magnus Berggren*
Affiliations : * Laboratory of Organic Electronics, Dept. of Science and Technology, Link?ping University, Norrk?ping, ? Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, # Duke-NUS Graduate Medical Schoolб donata.iandolo@liu.se

Resume : Previous studies have reported the use of 3D Polycaprolactone (PCL) macroporous scaffolds in bone tissue engineering applications, demonstrating their usefulness in promoting bone healing.1-3 In alternative studies conductive coating layers, used to provide electrical stimulation, were similarly shown to positively affect the proliferation of bone forming cells and their long-term functions.4 Here we report the use of vapor phase polymerization as a robust and reproducible technique for coating 3D printed PCL scaffolds with Poly(3,4-ethylenedioxythiophene):tosylate (PEDOT:tos). The surface and mechanical properties of the so developed conductive scaffolds have been investigated being these features crucial for their use in osteoinduction studies. Moreover, human fetal Mesenchymal Stem Cells (hfMSC) were showed to actively proliferate on the PEDOT:tos coated scaffolds. These preliminary results pave the way to further studies on the combined effects of electrical and topographical cues in bone tissue regeneration applications. References 1. Biomaterials (2014) 35, 5647-5659, 2. J. Biomed Mater Res A. (2010) 93, 4, 1358-1367, 3. Tissue Engineering: Part A (2011) 17, 19 2389-2397, 4. J. Mater. Chem. B, (2013) 1, 1439

V.4..3
09:50
Authors : Yaopeng Zhang*, Zhaobo Li, Qiangqiang Liu, Huili Shao
Affiliations : Donghua University, China

Resume : Bombyx mori silk is of great interest to people for its outstanding mechanical and biological properties. However, the traditional electrospun regenerated silk fibroin (RSF) scaffolds from aqueous solution were weak and had limited applications. This study was to fabricate reinforced scaffolds with well-aligned RSF fibers electrospun on a layer of native extracellular matrix, bladder acellular matrix graft (BAMG). The silk fibroin fibers were well-aligned as a grill in multiple layers. Both the BAMG and the grill structure significantly improved the tensile properties and suture retention of the composite scaffolds. This made the scaffolds can be sutured well with tissue during implantation. In vitro assay indicates that the scaffolds had a good biocompatibility. Porcine iliac endothelial cells (PIECs) were attached and proliferated well on the vascular endothelial growth factor (VEGF) loaded scaffolds compared with those without VEGF. Moreover, the grill-like structure guides PIECs well along the aligned fiber.

V.4..4
10:20
Authors : Min Hee Kim 1, Dong Su Im 1, Donghwan Cho 2, Oh Hyeong Kwon 2, Won Ho Park 1*
Affiliations : 1 Department of Advanced Organic Materials and Textile system Engineering, Chungnam National University, Daejeon, 305-764, Korea 2 Department of Polymer Science and Engineering, Kumoh National Institute of Technology Gumi, 730-701 Korea

Resume : Tissue engineering has potential to address this need through the combination of biomaterials, growth factors, and cells. Highly porous scaffolds are generally used as the substrate for anchorage dependent cells and to facilitate nutrient and metabolite distribution to guide cell growth leading to new bone tissue formation. Hydroxyapatite (HAP) has been investigated for bone replacement since this material mimics natural bone mineral features. HAP has been studied extensively in cell culture and possesses osteoconductivity. Regenerated Bombyx mori silk fibroin (SF) has excellent biological and mechanical properties, including biocompatibility, programmable biodegradability, and remarkable strength and toughness. One of the important physical forms for biomaterials is the formation of hydrogels, which has been extensively studied for a variety of polymers. The sol-gel transition depended on the concentration of the protein, temperature, and pH. In the SF hydrogel, random coil to β-sheet (physical cross-linking) structural transitions were noted during the process of hydrogelation. Due to the β-sheet formation, SF exhibits relatively slow degradation in vitro and in vivo, compared to collagen and many other biopolymers. In this study, the synthesis and characterization of bone-like mineral HAP into highly porous biodegradable silk fibroin scaffold with via chemical cross-linking reaction of SF by gamma-ray were investigated.

V.4..5
11:00
Authors : Franck TANCRET (1), Jingtao ZHANG (1,2), François PECQUEUX (1,2,3), Weizhen LIU (1,2), Pierre LEURET (2,4), Nathalie PAYRAUDEAU-LE ROUX (1,2), Pierre WEISS (2), Jean-Michel BOULER (2,4)
Affiliations : 1: Institut des Matériaux Jean Rouxel – Nantes (IMN), Université de Nantes, CNRS, France. 2: Laboratoire d’Ingénierie Ostéo-Articulaire et Dentaire (LIOAD), Université de Nantes, INSERM, France. 3: Graftys, France. 4: Chimie Et Interdisciplinarité, Synthèse, Analyse, Modélisation (CEISAM) , Université de Nantes, CNRS, France. franck.tancret@univ-nantes.fr

Resume : Calcium phosphates are widely used as resorbable bone substitutes under different forms, like Biphasic Calcium Phosphate (BCP) ceramics, Calcium Phosphate Cements (CPC), the latter being either purely inorganic cements or composite materials obtained by the addition of organic polymers and/or fibers. However, a number of technological issues still need to be addressed, in particular on the improvement of mechanical properties. The present lecture aims at reviewing recent works on the links between microstructure (porosity at different scales, nature and amount of polymer or fiber additives…) and several mechanical properties, may they concern elastic behaviour (Young’s modulus) or fracture behaviour (toughness and tolerance to damage), in relation with processing (strength and its statistical Weibull analysis). Ways to produce a variety of microstructures will be described (incorporating porogenic agents, changing the sintering temperature of BCP ceramics or the liquid-to-powder ratio in the CPC paste, adding cellulose ethers or fibers to CPC…) and their influence on mechanical properties will be discussed, in an attempt to propose strategies for material optimisation. In particular, it is shown that the addition of specific additives can improve fracture toughness, induce a good tolerance to damage, and may lead to interesting porous microstructures in view of biomedical applications.

V.4..6
11:30
Authors : Mathilde Giraudel1, Van Quang Le1, Faerber Jacques1, Andrea Cochis2, Lia Rimondini2,3, Leandro Jacomine4, Geneviève Pourroy1 and Adele Carrado1
Affiliations : 1 Institut de Physique et Chimie des Matériaux de Strasbourg IPCMS, UMR 7504 CNRS-ECPM-Université de Strasbourg, 23 rue du Loess BP 43, 67034 Strasbourg cedex 2 2 Department of Health Sciences; Università del Piemonte Orientale “Amedeo Avogadro”; Novara, Italy; 3 Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali; Firenze, Italy; 4 Institut Charles Sadron (ICS) CNRS - Université de Strasbourg (UPR 22). 23 rue du Loess, BP 84047. 67034 Strasbourg Cedex 2. France

Resume : We developed and patented a new method for improving osteointegration of titanium-based alloys [1]. Metallic artificial implants are widely used as medical devices to replace, support or enhance an existing biological structure. Titanium-based alloys possess advanced mechanical properties and excellent biocompatibility, but the bioactivity with the host has also to be improved. We obtained Ca-P /sodium titanate/ Ti-6Al-4V devices with good cell viability properties as well as good cohesion between the substrate and the coating. The implant surface was modified by mechanical, chemical and heat treatments, then a Ca-P layer was deposited onto the metal surface by sol-gel route. The coating has been characterized by X-ray diffraction, scanning electron microscopy performed on transverse section, and EDSX analysis. Nanoscratch tests showed a plastic deformation during the mechanical stress and no delamination of the coating was observed. SEM observations of residual grooves after the scratch with a maximal load of 100 mN showed the good adhesion of all Ca-P coatings onto the substrate. In vitro bioactivity of implants was test by Kokubo’s methods using the simulated body fluid. Finally, biological evaluations such as cell morphology and density as well as cell viability were performed. [1] Multilayer metallic materials with nanoporous calcium phosphate surface layers for medical implants Carrado et al. PCT Int. Appl. (2013), WO 2013068591 A1 20130516

V.4..7
12:10
Authors : Ahmed Salama, Andreas Taubert
Affiliations : Institute of Chemistry, University of Potsdam; ataubert@uni-potsdam.de

Resume : Cellulose/calcium phosphate hybrid materials were synthesized via an ionic liquid-assisted route. Scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, infrared spectroscopy, and thermogravimetric analysis/differential thermal analysis show that, depending on the reaction conditions, cellulose/hydroxyapatite, cellulose/chloroapatite, or cellulose/monetite composites form. Preliminary studies with MC3T3-E1 pre-osteoblasts show that the cells proliferate on the hybrid materials suggesting that the ionic liquid-based process yields materials that are potentially useful as scaffolds for regenerative therapies.

V.4..9
12:30
Authors : Paola Palmero1, Helen Reveron2, Laura Montanaro1 and Jérôme Chevalier2,3
Affiliations : 1Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi, 24, 10129 Torino, Italy; email: paola.palmero@polito.it 2Université de Lyon, INSA de Lyon, MATEIS CNRS UMR5510, 20 Avenue Albert Einstein, F-69621 Villeurbanne Cedex, France 3Institut Universitaire de France, 103 bd Saint-Michel, Paris 75005, France

Resume : LONGLIFE (“Advanced multifunctional zirconia ceramics for long-lasting implants”) coordinated by Prof. Jérôme Chevalier from INSA of Lyon, is a Collaborative European project in the frame of the 7th Framework Program. LONGLIFE aims at developing new zirconia-based oral and spine (lumbar inter-vertebral disc) implants, characterized by lifetime longer than 60 years and superior reliability as compared to previous biomedical devices. In order to reach this ambitious goal, efforts should be dedicated to improve the in-vivo stability of zirconia, by one side, and enhance the osseointegration capabilities of the implants in contact with bone, on the other. At the same time, the project aims at developing new ceramic-oriented designs for the implants as well as new multi-physic accelerated testing in vitro, able to reproduce more effectively the different degradation mechanisms and their interplay. In this work, the achievements concerning the design and development of new zirconia-based materials are presented, reached thanks to the synergies between Politecnico of Torino and INSA of Lyon. Among the technical oxide ceramics able to be used as structural biomaterials, zirconia-based ceramics are considered the best choice thanks to their excellent mechanical properties, biocompatibility and aesthetics. However, the current zirconia-based implants suffer from degradation under hydrothermal conditions (low temperature and presence of water), leading to surface degradation and possibly to fracture. Therefore, in order to increase the implants reliability, a strong effort is required to improve the zirconia stability in the presence of water, without decreasing its toughness and strength. The strategy chosen in LONGLIFE towards this goal is reported. Besides an innovative design of the composite material, a controlled synthesis process was used to produce multi-phase composites, having controlled architecture and composition. The results achieved demonstrate the effectiveness of this strategy towards the development of strong, tough and stable materials, fulfilling the objectives of the project.

V.4..10
14:00
Authors : Paola Palmero (1), Mariangela Lombardi (1), Krzysztof Haberko (2), Waldemar Pyda (2) and Laura Montanaro (1)
Affiliations : 1) Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi, 24, 10129 Torino, Italy; email: paola.palmeroolito.it; 2) Faculty of Materials Science and Ceramics, AGH University of Science and Technology, A. Mickiewicza 30 Ave., Krakow, Poland.

Resume : Thanks to its biocompatibility, bioactivity, high osteoconductive and/or osteoinductive behaviour, hydroxyapatite (HA) has been deeply investigated for the production of bone substitutes. Besides several techniques currently used to synthesize HA powders, an innovative route implies the extraction of natural HA from bio-wastes. This method offers both biological and economic advances over the traditional synthetic techniques. Among bio-wastes, fish, bovine and pig bones have been explored as HA raw materials. The extraction of carbonated HA occurred through thermal, subcritical water or alkaline hydrothermal processes. In this work, natural HA powder was extracted from the cortical part of long pig bones, by treating at 100 °C with a NaOH water solution (4 M) for 48 hours. In order to remove the remaining sodium hydroxide, the material was then carefully washed into distilled water until a pH of 7 of the filtrate was reached. The dried powder was submitted to washing steps in distilled water, inducing de-carbonation by exploiting the high solubility of Ca(OH)2 in pure water. Washing steps were prolonged until a pH of 7 in the filtrate was reached. Starting from this natural HA powder, macro-porous ceramic materials have been prepared by a gel-casting process combined with a sacrificial template method. In particular, agar was used as natural gelling agent and polyethylene (PE) spheres as pore former. The sintered components were characterized by tailored macro- and micro-porosity features, as required for the development of engineered ceramic scaffolds.

V.4..11
14:45
Authors : Anna Rybachuk (2), Liudmyla Rieznichenko (1), Vladislav Malanchuk (2), Tamara Gruzina (1), Zoya Ulberg (1)
Affiliations : (1) F.D. Ovcharenko Institute of Biocolloidal Chemistry of NAS of Ukraine. Vernadskogo av., 42, Kyiv-03142, Ukraine; e-mail: Reznichenko_LS@mail.ru (2) Bohomolets National Medical University, Ukraine

Resume : The frequency of surgical septic postoperative complications in dirty wounds, which include a group of surgical interventions for purulent processes of maxillofacial area, averages more than 30-40%. This index is growing by every year due to increasing of widespread of multidrug-resistant pathogen strains and weakening of the immune status of patients. The same causes are among the leading in the case of the emergence and development of inflammatory complications in dental implantology because of a high risk of surgical wound infection by oral microflora. So, the prevention and treatment of such complications in the practice of maxillofacial surgery and dental implantology are important today. The effectiveness of silver nanoparticles (AgNP) and their combination with gold nanoparticles (Ag/AuNP) as new effective antimicrobial substances for maxillofacial surgery and dental implantology, in comparison with traditional antimicrobial substances has been studied. High bactericidal effectiveness of the studied metal nanoparticles (AgNP and Ag/AuNP) has been revealed in vitro against wide spectra of pathogens including multidrug-resistant ones (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Haemophilus influenzae, Micrococcus, Candida albicans etc.). All pathogens have been isolated from patients of the Department of Maxillofacial Surgery. High antimicrobial and anti-inflammatory effectiveness of AgNP and Ag/AuNP substances, in comparison with traditional antimicrobial drugs, has been shown in vivo on the model of abscesses of submandibular area using microbiological, biochemical and histological methods. Wistar rats have been used for modeling abscesses of submandibular area by means of subcutaneous injection in the submandibular area of the suspension containing clinical isolate of Staphylococcus aureus and active carbon powder.

V.4..13
15:00
Authors : Vitalii Dubok
Affiliations : I.M.Frantsevich Institute for Problem of Materials Science NASU

Resume : By simulation of the biological process of synthesis and morphology of phosphates in the body the bioactive multiple doped nanoceramics have been developed which have 1000 - 10 000 times larger cross section of thermal neutron capture (or neutron with selected energy range), designed for neutron capture therapy of cancer tumors. The nanoceramics can be fixed at a predetermined position of the body through biochemical bonding and is fully bioresobable during 6 to 12 months after implantation.

V.4..14
Start atSubject View AllNum.
09:15
Authors : R. Tenne
Affiliations : Weizmann Institute; reshef.tenne@weizmann.ac.il

Resume : This presentation is aimed at demonstrating the progress with the synthesis and characterization of new inorganic nanotubes (INT) and fullerene-like (IF) nanoparticles (NP) from 2-D layered compounds. Two important categories of new IF/INT nanostructures will be discussed in particular: 1. Synthesis of Doped IF/INT of WS2 (MoS2) by rhenium and niobium; 2. Synthesis of IF and in particular INT from the ternary misfit compounds, like PbS-TaS2, GdS-CrS2. The synthesis of 1-D nanostructures from this vast group of layered materials is particularly promising. Re-doped IF-MoS2 NP exhibit superior solid lubrication behavior in different environment and can find numerous applications in e.g. medical technology, which will be briefly demonstrated. Major progress has been achieved in elucidating the structure of INT and IF using advanced microscopy techniques, like aberration corrected TEM and electron tomography. Applications of the IF/INT as superior solid lubricants and for reinforcement of polymer nanocomposites, which gained a lot of momentum in recent times, will be briefly discussed. Few recent studies indicate that this brand of nanoparticles is not-toxic and biocompatible. With expanding product lines, manufacturing and sales, this generation of superior lubricants is becoming gradually a commodity.

V.5..2
11:00
Authors : Yan Li, Xin ZHANG*
Affiliations : National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, CHINA, xzhang@ipe.ac.cn

Resume : Gene silencing via RNA interference (RNAi) has been demonstrated as a great potential therapeutic agent for cancer treatment. The emerging RNAi therapy delivers gene-silencing RNAs to the cytoplasm where they recognize and degrade the complementary target mRNA in a sequence-specific manner at a post-transcriptional level. However, safe and efficient transduction of siRNAs to target tissues and cells remains a major challenge for clinical trials. Therefore, we have developed several functional lipid-based nanoparticles as carriers for siRNA delivery to overcome the disadvantages mentioned above. For instance, many kinds of nanosized cationic delivery systems have been developed for siRNAs delivery via electrostatic interaction, such as cationic polymers, cationic lipids and so on. However, due to low charge density and stiff backbone structure, siRNA with 21-23 bp nucleotides has inherently poor binding ability to cationic polymers and lipid carriers, which results in low siRNA loading efficiency. In order to achieve high loading efficiency for gene transfection, cationic polymers and lipids must be used in an excess amount, whereas they often exhibit severe cytotoxicity. In this study, we report chemically conjugated siPlk1-phospholipids enveloped hybrid nanoparticles (siPlk1-PCNPs) for siRNA delivery to overcome the siRNA’s stiff backbone structures and enhance siRNA loading efficiency.

V.5..6
11:40
Authors : Chao Zhang, Jingru Shi, Tingting Fang, Huili Shao, Xuechao Hu, Yaopeng Zhang*
Affiliations : State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China; zyp@dhu.edu.cn

Resume : Regenerated silk fibroin (RSF)/graphene oxide (GO) hybrid silk fibers were dry-spun from a mixed dope of GO suspension and RSF aqueous solution. The rheological properties of the RSF/GO spinning dope were varying with the GO content. Synchrotron Radiation Wide Angle X-ray Diffraction and FTIR results showed that the addition of GO could confine the crystallization of silk fibroin which lead to the decrease of crystallinity, the smaller crystallite size and more formation of interphase zones in the artificial silks. Synchrotron Radiation Small Angle X-ray Scattering were also applied in the hybrid silks and hybrid solutions to study the interphase zones formed by the complex interaction between silk fibroin and GO flakes. An outstanding reinforcement was observed for the dry-spun hybrid silk fibers, of which the breaking stress reached 341.5 MPa.

V.5..8
14:50
Authors : Paolo Perna, Francisco J. Terán, Julio Camarero, and Rodolfo Miranda
Affiliations : IMDEA NANOSCIENCE, c/ Faraday 9, Campus de Cantoblanco ES-28049, Madrid, Spain; paolo.perna@imdea.org

Resume : Magnetoelectronics at the nanoscale provides new strategies to tackle issues concerning to life sciences and healthcare: from recognition of molecules to therapeutic health treatments. Magnetic structures can be exploited to detect molecules, bacteria and cells or to improve the functionalities of artificial biological tissues and finally may be directly employed to drug delivery and to kill cancers. However, this is only possible through a smart bio-functionalization of the magnetic objects, and having control onto their magnetic properties. Nanomagnetism applied to Nanomedicine appears thus as an emerging and multidisciplinary discipline that aims to provide personalized and more efficient tools to detect and treat health diseases, as delivering cancer treatments at the right place, at the right dose and at the right moment. This novel technology offers clear advantages over conventional techniques that can suppose a real breakthrough in biomedical research, and health care. Within this context, I illustrate different examples of bio-sensing and bio-medical applications based on magnetic nanomaterials.

V.5..14
15:10
Authors : J. Jouhannaud1, I. Miladi2, A. Garofalo1, P. Poulet2, D. Felder-Flesch1, G. Pourroy1
Affiliations : 1Institut de Physique et Chimie des Matériaux de Strasbourg IPCMS, UMR 7504 CNRS-ECPM-Université de Strasbourg, 23 rue du loess BP 43, 67034 2; 2Laboratoire des Sciences de l'Ingénieur, de l'Informatique et de l'Imagerie - ICube - UMR 7357 Institut de Physique Biologique Faculté de Médecine 4, rue Kirschleger 67085 Strasbourg cedex. Corresponding author: genevieve.pourroy@ipcms.u-strasbg.fr

Resume : Nanoparticles combining optical and magnetic signals are promising for the detection of sentinel nodes by hand-held probes in cancer surgery. Now, radioactive colloids (RuS labelled with 99mTc) or/and Vital Blue dye are injected around the primary tumour and detected by nuclear probe or the eye respectively. The aim of our work is to replace the radioactive colloids by magnetic colloids by developing and optimizing dendronized iron oxide nanoparticles. The nanoparticles were produced in an aqueous basic media and sorted by varying the ionic force, temperature and magnetic field parameters. The dendrons are either of first or second generation and bear a Patent Blue dye and/or a fluorescent dye. The suspensions have been characterized by dynamic light scattering, magnetic susceptibility and the amount of incorporated Patent Blue/fluorescent dye analyzed. We show that the structure and the composition of the aggregates strongly impact the optical and magnetic properties of the suspensions and therefore are critical for optimizing their detection sensitivity. Major attention was given to the effect of the dendron length, the grafting ratios, as well as to the electrostatic interactions between the dendron and the dye. Finally, in-vivo experiments on mice have been performed to determine the biodistribution. This work was supported by the European Union in the framework of the program «Nano@matrix» INTERREG IV Upper Rhine Valley.

V.5..15
16:00
Authors : Ingrid Hilger
Affiliations : Institute for Diagnostic and Interventional Radiology, Jena University Hospital, Erlanger Allee 101. D-07747 Jena, Germany

Resume : Currently, therapy of cancer is mainly based on different modalities, which are distinctly selected depending on the cancer stage and tumor entity to be treated. Most of them are not highly specific, leading to side effects as well as long and painful patient recovery. Therefore, new treatment modalities have been proposed. Among them hyperthermia is considered to be a promising tool which basically addresses the transient rise of temperature above 37 °C leading to tumor cell death due to distinct changes of the tumor pathophysiology. Two different concepts of heating have been followed so far: hyperthermia (temperatures between 41 and 45 °C Experience from the clinical and preclinical studies has shown that a dedicated combination of hyperthermia with established oncologic modalities could markedly increase the therapeutic outcome and patient compliance (e.g. [6]). In this context, the synergy of therapeutic modalities with regard to breast and pancreatic cancer cells at early stages of tumor development are being evaluated in a collaborative European project (Multifunctional Nanotechnology for selective detection and Treatment of cancer, MultiFun, 7th framework program). Hereto, dedicated nanoparticle formulations in terms of their heating capabilities, morphologic and magnetic features, in vitro biocompatibility in isolated cells, pharmacokinetic properties and their therapeutic efficacy in vivo are being investigated.

V.5..16
16:25
Authors : Ovidiu Crisan
Affiliations : National Institute for Materials Physics, Bucharest, Romania; ocrisan@yahoo.com

Resume : FePt system attracts currently a great deal of interest for applications as future RE free permanent magnets. Among the key issues to be solved one may count the decreasing of the ordering temperature and improvement of magnetic behaviour. For that purpose we have studied the effect of Ag addition into FePtB melt spun ribbons combined with a nitriding post synthesis procedure, aimed at refining the microstructure and enhancing the magnetic performances. Deep structural characterization by transmission electron microscopy, electron diffraction, energy dispersive X-ray spectroscopy and X-ray diffraction allowed us to observe the morphology and to correctly assign and identify the nature of the main granular phases observed. Nitriding procedure is shown to enhance the (001) texturing and the degree of ordering of the L10 FePt phase, as well as strong increase of coercivity, compared to the as-cast state. These changes are interpreted in terms of mechanisms of Ag segregation towards intergranular region associated to N diffusion and creation of vacancies that favor consistently the process of ordering the FePt grains into the L10 tetragonal phase.

V.5..17
16:40
Authors : F. Bertorelle, M. Pinto, G. Fracasso, V. Amendola, M. Colombatti, M. Meneghetti
Affiliations : Department of Chemical Sciences, University of Padova, Padova (Italy) F. Bertorelle; V. Amendola; M. Meneghetti; Dept. of Pathology - Sect. Immunology, University of Verona, c/o Policlinico GB Rossi, Verona (Italy): M. Pinto; G. Fracasso;M. Colombatti; fabrizio.bertorelle@studenti.unipd.it

Resume : Designing and synthesis of multifunctional nanoparticles for biological application is an actual challenge in which many research groups are now involved. Most of these studies are focused on the lowering the detection limit of cell expressions with respect to current diagnosis techniques. Our results start from the synthesis of naked AuNPs and FeOxNPs obtained with Laser Ablation synthesis in water. Nanoparticles are then combined in a core-shell-satellite type structure using MPTMS. SERS properties of these nanostructures are obtained with a dye embedded in the MPTMS shell. To perform immunomagnetic sorting, satellite AuNP are functionalized with an antibody for membrane prostatic antigen (PSMA). This type of nanostructures show promising results in immunomagnetic sorting and detection of prostate cancer cells. Associating different dyes with different antibodies there is also the possibility of performing SERS measurement with a multiplexed approach. 1 M. Meneghetti, et al. Small,(2012) 8, 3733 2 F. Bertorelle, et al. J. Phys. Chem. C (2014) 118, 14534 3 V. Amendola, et al. Small (2014) 10, 2476

V.5..18
17:40
Authors : Xiangyang Shi
Affiliations : College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China. E-mail: xshi@dhu.edu.cn

Resume : The hydrothermally synthesized Fe3O4 NPs usually have a controllable size, narrow size distribution, and tunable magnetic properties, offering many opportunities in the field of biomedical imaging applications. This talk will be focused on the recent progress of hydrothermal synthesis and functionalization of iron oxide nanoparticles for magnetic resonance (MR) imaging applications developed in our group. Our work has been involved in the use of modified hydrothermal routes to generate surface-functionalized Fe3O4 NPs with good water dispersibility, colloid stability, and relatively high r2 relaxivity. In particular, the hydrothermally synthesized Fe3O4 NPs coated with branched polyethyleneimine were able to be further surface functionalized with targeting ligands (e.g., folic acid and hyaluronic acid), enabling effective tumor MR imaging with high specificity via receptor-mediated active targeting pathway. What’s more, the hydrothermal route was also used to synthesize Fe3O4@Au composite nanoparticles for dual mode MR and computed tomography (CT) imaging and photothermal therapy of tumors.

V.5..21
 
Special Poster Session –Panel Discussions “Face-to-Face” Invited Lecturers/Presenters – Participants : ”Biological, Biomimetic synthesized NPs, Functilized inorganic NPs, nanotubes and Nanocarbon molecules in complex nanomaterials with potential biomedical functions. Magnetic NPs: nanomagnetism and bio –sensing, -medical functions" Chair/Organizer Dr.Paolo Perna. Assisted by MS student Elena Yunda, eny@tpu.ru
18:00
Authors : Kyong-Hoon Choi, Kyu-Dong Lee, Un-Ho Kim, Bong-Ju Park, Jin-Seung Jung
Affiliations : Department of Electrical & Biological Physics and Plasma Bioscience Research Center, Kwangwoon University, 20 Kwangwoongil, Nowon-gu, Seoul, 139-701, Korea; Department of Chemistry, Gangneung-Wonju National University, Gangneung 210-702, Korea; jjscm@gwnu.ac.kr

Resume : In addition to the increasing number of nanotechnology applications in medical diagnostics and therapeutics, the utilization of nanomaterials in consumer products has recently become more important. Among them, the spinel ferrite materials have widely been paid attention due to their current and future applications not only in the field of catalysts but also in the field of biology and medicine, such as diagnostics, biosensing, therapeutics, drug delivery and targeting. In this study, biocompatible multifunctional particles (CoFe2O4@HP) of well-defined sizes (60, 133, 245, 335 nm) were fabricated to be used as photodynamic therapeutic agents for cancer cells. Functionality of photodynamic therapy (PDT) is provided by hematoporphyrin (HP) which generates singlet oxygen with high quantum yield by photodynamic treatment. The HP molecules are covalently bonded to the surface of CoFe2O4 nanoparticle in various sizes of CoFe2O4 nanoparticle.The magnetic properties of cobalt ferrite (CoFe2O4) particles have been finely adjusted by controlling the size of the primary CoFe2O4 nanograins and the secondary superstructure composited particles formed by an aggregation of the nanograins. The prepared CoFe2O4@HP particles with various sizes exhibit high water solubility, good magnetic resonance imaging (MRI) and biocompatibility without any cytotoxicity. Especially, anticancer activities of CoFe2O4@HP present remarkable photodynamic anticancer efficiency via apoptosis in PC-3 cells, which showed particle size and dose dependent toxicity. This size dependent effect is determined by the particle specific surface area. These results indicate that CoFe2O4@HP are suitable for effective photodynamic therapy (PDT) and have potential as therapeutic agents for MRI based PDT, because they have a high value of saturation-magnetization and superparamagnetism

V.V-P3..1
18:00
Authors : P. Perna* (1), D. Maccariello (1,2), J. L. F. Cuñado (2), F. Ajejas (1), M. A. Niño (1), J. Camarero (1,2) and R. Miranda (1,2)
Affiliations : (1) IMDEA Nanoscience, c/ Faraday, 9 Ciudad Universitaria de Cantoblanco 28049, Madrid (Spain); email: paolo.perna@imdea.org (2) D.F.M.C., Universitad Autonoma de Madrid, 28049 Madrid, Spain paolo.perna@imdea.org

Resume : The understanding of the magnetoresistance (MR) responses as related to the way how the magnetization reverses in any kind of magnetic material is of great interest, not only for fundamental study, but importantly also for the improvement of the current spintronics technology. In this work, we demonstrate that by having direct experimental access to the magnetization vector during the reversal (by using a vectorial-Kerr magnetometry) we can predict the magnetoresistive signals. We demonstrate so in spin-valve [1] and in its basic constituents, i.e. single FM layer [2] and bilayer FM / AFM. We performed RT angular and field resolved M-H loops and the corresponding simultaneous resistance changes, and demonstrate that the R(H) curves depend exclusively on the reversal processes (ultimately dictated by magnetic anisotropy of the system). Very importantly, from the vectorial-resolved M-H we can experimentally determine the relative angle between the magnetization vectors of the two FM layers in spin-valve and the magnetic torque and the angle enclosed between the magnetization vector and the applied current direction in a single FM layer. This allows for the simulation and prediction of the giant magnetoresistance (GMR), anisotropic magnetoresistance (AMR) and Planar Hall Effect (PHE) in the whole angular range and for any magnetic field values. [1] P. Perna et al. Phys. Rev. B 86, 024421 (2012) [2] P. Perna et al. Appl. Phys. Lett. 104, 202407 (2014)

V.V-P3..3
18:00
Authors : A.D Crisan, O. Crisan, K. von Haeften, A.M. Ellis, C. Binns
Affiliations : National Institute for Materials Physics, PO Box MG-7, 077125, Bucharest-Magurele, Romania Department of Physics and Astronomy, University of Leicester, LE1 7RH Leicester, UK; ocrisan@yahoo.com

Resume : A novel method of synthesis of cluster-assembled magnetic nanoparticles is presented and the properties of the obtained gas-stabilised monodispersed Fe clusters are reported. A home made UHV chamber connected to a multiple cluster source, is presented. The technique uses rare gas flow (Ar) with that captures evaporated metal vapours and sputters the condensed clusters in-situ onto suitable chosen substrates. Extremely rigorous control of the size distribution and cluster density is achieved withthis technique. Cluster size is simply controlled by the vapour pressure of the metal source inside the pick-up chamber. Detailed analysis of the transmission electron micrographs of the Fe clusters onto C coated Cu grids shows that there is a simple linear relationship between average metal cluster size and metal vapour pressure during deposition. Magnetic properties are investigated and correlated with the cluster structure. Enhanced coercivity is observed for the Fe clusters and attributed to strong planar magnetic anisotropy. Applications of core-shell clusters as hyperthermia therapy agents is discussed

V.V-P3..4
18:00
Authors : Igor Kobasa, Iryna Kondratyeva
Affiliations : Yuriy Fedkovych Chernivtsi National University,Chernivtsi, Ukraine

Resume : Photocatalyst semiconductor titanium dioxide is widely utilized as a self-cleaning and self disinfecting material for surface coating in many applications. It has a more helpful role in our environmental purification due to its nontoxicity, photoinduced super hydrophobicity and antifogging effect. Titanium dioxide used frequently in cosmetics, pharmaceutical, paint and paper industry. Recently the interest of TiO2 increased to the environmental, medical and biological application. The materials based on titanium dioxide can be used to create of antibacterial ceramic, paint and varnish coating and packing with antibacterial properties. It was studied the antibacterial activity of samples highly porous titanium dioxide. They were obtained of high temperature (700-1100 oC) by hydrolysis of titanium tetrachloride vapor in the air-hydrogen flame. Research of antibacterial activity of TiO2 performed by a diffusion method (disk methods) according to ISO 27447: 2009. It was shown that the samples of suspensions titanium dioxide (0.1-10.0 %) are exhibit antibacterial activity to bacterias Escherichia coli and Staphylococcus aureus. It was observed a complete neutralization of colonies of microorganisms Escherichia coli while lesion area of bacteria Staphylococcus aureus was lower. In both cases, the effect of antibacterial action increases with the concentration of TiO2.

V.V-P3..6
18:05
Authors : Yaopeng Zhang*, Chao Zhang, Qingfa Peng, Lingyue Cai, Huili Shao
Affiliations : State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China; zyp@dhu.edu.cn

Resume : Animal silks from spider and silkworm attract many people’s attention due to their outstanding mechanical properties and the smart spinning method of the animals. In this paper, several biomimetic spinning methods for spider silk and silkworm silk were designed. By mimicking the dry-spinning method of silkworm and spider, regenerated silk fibroin (RSF) aqueous solution was used as spinning dope and spun in air directly at room temperature. The as-spun fiber was then post-drawn in ethanol aqueous solution to induce the conformation transition of RSF. The oriented crystalline and amorphous regions of the silk fibers contribute to the remarkable mechanical properties of the artificial silk. By mimicking the functions of the spinning apparatus of spider and silkworm, ion and protein concentrations in the RSF aqueous solution were adjusted in microfluidic chips with multiple channels. Inspired by the shape and dimensions of the natural spinning apparatus, a microfluidic chip was designed and applied to the studies of aggregation mechanism of silk fibroin in micro-channel. Moreover, the dry-spun/electrospun fibers of RSF were also toughened or reinforced by mimicking the core-shell structure of natural animal silks, adding silk sericin, graphene oxide, TiO2 nanoparticles in spinning dopes and changing the collecting method in electrospinning process.

V.V-P3..8
18:05
Authors : Ohad Goldbart1, Anastasia Sedova1,2, Lena Yadgarov1, Rita Rosentsveig1, Dmitry Shumalinsky3, Leonid Lobik3, H. Daniel Wagner1 and Reshef Tenne1
Affiliations : 1. Department of Materials and Interfaces, Weizmann Institute, Rehovot 76100, Israel 2. Azrieli - College of Engineering Jerusalem, P.O.Box 3566, Jerusalem, 9103501, Israel 3. Urology Department, Barzilai Medical Center, Hahistadrout St. 2, Ashkelon 78278, Israel

Resume : Insertion of endoscopes and other medical devices to the human body are ubiquitous, especially among aged males. The applied force for the insertion/extraction of the device from the urethra must overcome the endoscope (or catheter)-surface human-tissue interaction. In daily practice a gel is applied on the endoscope surface, in order to facilitate its entry to the urethra, providing also a local anesthesia. In the present work MoS2 nanoparticles, with fullerene-like structure and MoS2 doped with minute amounts of rhenium atoms, have been added to a commercial gel, in order to reduce the metal\catheter-urethra interaction and alleviate the potential damage to the epithelial tissue. In order to compare between the different gels a urethra model was designed and fabricated, which allowed a quantitative assessment of the applied force for extraction of the endoscope from a soft polymer-based ring. It is shown that the Re-doped nanoparticles reduce the traction force used to retrieve the metallic lead of the endoscope or the catheters from the soft ring by a factor close to three times with respect to the original gel. The mechanism of the mitigation of both friction and adhesion forces in these systems by the nanoparticles is discussed.

V.V-P3..10
 
Invited Special Poster Session – Panel Discussions on “Smart interfaces functionality and biomedical frontiers” : “Face-to-Face” Invited Lecturers/Presenters – Participants. Invited Chief/Organizer Dr. Peilen Chen, Center for Applied Sciences Academia Sinica, Taiwan, peilin@gate.sinica.edu.tw
18:10
Authors : Yu-Sheng Hsiao,Chiung-Wen Kuo, Peilin Chen
Affiliations : Department of Materials Engineering, Ming Chi University of Technology, Taipei, Taiwan Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; peilin@gate.sinica.edu.tw

Resume : Here we develop a universal solution-processing approach for producing three dimensional (3D) conducting polymer-based bioelectronic interfaces (BEIs), which can be integrated on chips for rare circulating tumor cell (CTC) isolation and detection. Based on the chemical oxidative polymerization and modified poly(dimethylsiloxane) (PDMS) transfer printing technology, the poly(3,4-ethylenedioxythiophene) (PEDOT)-based micro/nanorod array films can be fabricated with topographical and chemical control through the chemical oxidative polymerization. This 3D PEDOT-based BEI film features the advantageous characteristics: (1) diverse dimensional structures (tunable from the microscale to the nanoscale), (2) varied surface chemical properties (tunable from nonspecific to specific), (3) high electrical conductivity, and (4) reversible electrochemical switching, and (5) high optical transparency. Furthermore, we integrated this 3D PEDOT-based BEI on chips, which exhibited optimal cell-capture efficiency from MCF7 cells was approximately 85%; featured highly efficient performance for the cell isolation of rare CTCs with minimal contamination from surrounding nontargeted cells (e.g., EpCAM-negative cells, white blood cells); preserved the cell viability with negligible effect on cells. According to the electric cell-substrate impedance sensing concept, the 3D BEI-based device was also demonstrated as a high sensitive and specific liquid biopsy tool for rapid CTC purification, detection and characterization. Therefore, it is conceivable that use of this platform will meet the requirements on developing for the next-generation bioelectronics for biomedical applications.

V.V-P4..1
18:10
Authors : Chiung Wen Kuo, Yi-Wen Chen, Peilin Chen
Affiliations : Research Center for Applied Sciences, Academia Sinica, Taipei-115, Taiwan, R.O.C.; peilin@gate.sinica.edu.tw

Resume : Circulating tumor cells (CTCs) in the bloodstream are exceptionally rare. As a result, efficient and specific isolation and capture of CTCs from peripheral blood are important not only for disease diagnosis, but also for patient treatment at early stages. In this work, we reported a platform comprised of electrospun nanofibers and a microfluidic device to isolate CTCs in whole blood with high efficiency and specificity. The nanofibers with high surface-to-volume ratio were aligned or randomly electrospun and embedded in the microchannels. The fiber diameter and biotin conjugation were investigated and optimized by studying their effects on the CTCs capture efficiency. In addition, both microfluidic devices contained aligned and random nanofibers showed low yield of nonspecific binding of CTCs. As compared with the aligned device, the random device yielded higher capture efficiency and recovery of 97% from whole blood. Results showed that high numbers of captured CTCs were viable, making them an ideal platform for subsequent molecular- or cellular-level studies.

V.V-P4..2
18:10
Authors : Qichao Pan1, Yaqiong Zhang1, Hsing-An Lin2, Shyh-Chyang Luo3, Hsiao-hua Yu4, Haichao Zhao5, Bo Zhu1
Affiliations : 1 State Key Lab for Modification of Chemical Fibers and Polymer Materials & College of Materials Science and Engineering, Donghua University, 2999 North Renmin Road, Songjiang, Shanghai, 201600, China; 2 Responsive Organic Materials Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan; 3 Department of Materials Science and Engineering, National Cheng Kung University, Tainan,Taiwan,70101; 4 Institute of Chemistry, Academia Sinica, 128 Academic Road, Sec. 2, Nankang, Taipei , Taiwan, 11529 5Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo,315201, China; bzhu@dhu.edu.cn

Resume : Electrical conducting polymers (ECPs) are promising as interfacial materials to provide a combination of the electrical, biochemical and topological requirements for neural interfaces. ECPs are electrical/ionic conductive and effective at electron-ion transition over device-tissue boundary. Pioneer works have demonstrated the efficient utilization of ECPs to modulate cellular activities (including cell adhesion, migration, DNA synthesis, protein secretion, etc.) upon electrical stimulation. Nowadays, many research interests on bioelectronic devices have shifted to ethylenedioxythiophene (EDOT)-based polymers because of its long-term electro-stability in aqueous solution. In the present research, hydrophilic and bio-functionalized ethylenedioxythiophene (EDOT) building blocks were designed on mimicking natural bio-membranes, and chemically synthesized. The functionalized EDOT building blocks were electrodeposited as cell-nonstick or stick polymer thin films. The surface properties of the thin films could be easily switched from cell binding to cell resistant by deposition of a new layer of EDOT polymer onto the existing surface. This feature allow us to integrate the material assembly process with top-down lithographic process, thereby fabricating patterned all conducting polymer biointerface to spatially define the cell behaviors. The spatial control on attachment of cell could be available at a single cell if the binding domain was small enough. Finally, this platform was further employed to integrate spatial arrangements, surface functionality and electric stimulation to direct cell polarity, proliferation and differentiation. The spatial definition of cells at cellular level on all-conducting-polymer interface would not only help us to understand how electrical stimuli modulates cellular activities of neurons, but also could be adopted to guide nerve regeneration.

V.V-P4..3
18:10
Authors : O.Zeggai; H.Zeggai; A. Ould-Abbes; M. Belarbi
Affiliations : Research Unit of Materials and Renewable Energies (URMER), University Abou Bakr Belkaïd, B.P. 119, Tlemcen, Algeria; zeggai_oussama@yahoo.com

Resume : In recent years, a new form of carbon nanotube electrode spread like promising nanoelectronic devices based on carbon and for biological and medical applications. Because of their physical property, chemical and electrical. Carbon nanotubes can be effectively used as electrochemical sensors. The integration of carbon nanotubes provides a good solid support for the immobilization of enzymes. Determining glucose levels using biosensors, especially in medical diagnostics and in food industries. This action provides high accuracy and rapid detection rate. The purpose of this study includes the presentation of the transistor-based biosensor field effect structure with a grid of carbon nanotubes for detection of glucose with an analytical study of biosensor, and in this model we present a relationship combines between the glucose concentration and one of transistor parameters. .

V.V-P4..5
18:10
Authors : Maria Vorobets, Heorhii Vorobets
Affiliations : Yuriy Fedkovych Chernivtsi National University; g.vorobets@chnu.edu.ua

Resume : Organic acids and their salts (sorbic acid (E200) and sodium sorbate, potassium sorbate, calcium sorbate (E201, E202, E203), benzoic acid (E210) and sodium benzoate, potassium benzoate, calcium benzoate (E211, E212, E213), acetic acid (E 260) and sodium acetate, calcium acetate, ammonium acetate (E262, E263, E264), ascorbic acid (E300) and sodium ascorbate, calcium ascorbate, potassium ascorbate (E301, E302, E303), etc.) are widely used as food additives (preservatives, antioxidants) in the food industry and in cosmetics, pharmacy and medicine. However, beyond the limiting doses they can have harmful effects on the human body that actualizes on developing rapid methods of control of their content in food products. When sampling for analysis important point is to ensure the necessary concentration of controlled substances according to the sensitivity of the measuring channel information-measuring system. The purpose of this study was to investigate the sorption properties of porous silicon on some of these organic acids and their salts. Using double-stage processing of silicon wafers for the active porous silicon surface: 1) chemical treatment in ammoniac-peroxide and acid-peroxide solutions for various combination options; 2) formation of anodic porous silicon surface at different values of anode current. The research of the sensory properties of porous silicon surface was performed photometric, spectral and other methods. The sensitivity of the optical solar cells was provided at a pico Ampere. For rapid analysis of the results used mobile computerized measuring system. The error of measurements and data processing did not exceed 8%. According to the research proposed and optimized structure of the measuring cell and method of rapid analysis of some organic acids and their salts-food additives. It is shown that in the study of the sorption properties of porous silicon on organic compounds there is a relationship between the presence of active -O-O- and -O-H groups and chemical surface treatment method of silicon wafers in ammoniac-peroxide or acid-peroxide solutions.

V.V-P4..11
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Invited One Day Session “Smart interfaces functionality and biomedical frontiers” : Invited Chairs/Organizers: Dr Peilin Chen (Center for Applied Sciences Academia Sinica, Taiwan, peilin@gate.sinica.edu.tw ) , Prof. Bo Zhu (Donghua University, Shanghai, China, bzhu@dhu.edu.cn )
08:30
Authors : Peilin Chen
Affiliations : Research Center for Applied Sciences, peilin@gate.sinica.edu.tw

Resume : An optically transparent poly(3,4-ethylenedioxythiophene) (PEDOT) based organic electronic devices have been developed to investigate the behavior of human mesenchymal stem cell (hMSC) and to sense and to capture circulating tumor cells. We first conducted a control experiment on electrical cell-substrate impedance sensing (ECIS) device by culturing the hMSC on the chip. According to our result, the impedance increased reflected the hMSC proliferation, attachment and motility during the first 16 hours of cell culture. In order to control the differentiation of human mesenchymal stem cell (hMSC) on chip, we also developed all-solution-processed multifunctional organic devices, comprising reduced graphene oxide (rGO) and dexamethasone 21-phosphate disodium salt (DEX) drug loaded poly(3,4-ethylenedioxythiophene) (PEDOT) microelectrode arrays on indium tin oxide glass, that can be used to manipulate differentiation. In our devices, the rGO micropatterns were used as the adhesive coating to attract the adhesion of hMSC cells whereas PLL-g-PEG coated PEDOT electrodes served as the anti-adhesive coating where no hMSC cells can attach. In addition, the PEDOT electrodes also work as drug releasing components where control DEX release from PEDOT matrix can be achieved via cyclic potential stimulation (CPS). To capture the circulating tumor cells, we fabricated 3D PEDOT-based micro/nanorod array, which can be further surface-grafted with capture agents for directed specific recognition to study the cell–substrate interactions on bioelectronics interfaces (BEIs). This BEI platform features the advantageous characteristics: (1) diverse dimensional structures (tunable from the microscale to the nanoscale), (2) varied surface chemical properties (tunable from nonspecific to specific), (3) high electrical conductivity, and (4) reversible chemical redox switching. Furthermore, through systematic studies of PEDOT systems, we explore the effects of both chemistry and topography on the circulating tumor cell (CTC)-capture performance. The 400 nm PEDOT pillars exhibited the optimal cell-capture efficiency; it could be used to isolate CTCs with minimal contamination from surrounding nontargeted cells (e.g., EpCAM-negative cells, white blood cells) and negligible disruption of the CTCs’ viability and functions. It is conceivable that PEDOT-based micro/nanorod array films function as a critical therapeutic intervention for monitoring tumor progression and metathesis, providing valuable insight into the use of electronics for tissue engineering and regenerative medicine.

V.6..1
08:50
Authors : Bo ZHU
Affiliations : State Key Lab for Modification of Chemical Fibers and Polymer Materials & College of Materials Science and Engineering, Donghua University, 2999 North Renmin Road, Songjiang, Shanghai, 201600, China; bzhu@dhu.edu.cn

Resume : Immunogenic scar formation would insulate electrodes from targeted cells and tissues, thereby reducing the lifetime of the bioelectronic devices. Nonspecific interactions could make electrodes binding non-targeted cells, and lead to the poor selectivity of biosensors. Ideally, an electrode material capable of electrically interfacing with cells selectively and efficiently would be integrated without being recognized by the immune system or nonspecific interaction to cells. We developed a series of biomimetic conducting polymer from zwitterionic and cell-targeted ethylenedioxythiophenes (EDOTs), which specifically interact with cells on a protein-resistance background. The predominant presence of zwitterionic side-groups on surface prevent enzyme and cell binding, allowing the polymers to functionalize in aqueous buffer for an extended period of time and preventing nonspecific interaction. The cell-targeted peptides, proteins or polymer chains on surface ensure specific interaction with desired cells. The further introduction of stimuli-responsive bio-conjugation linkage or polymer chains allows a stimuli-responsive cell capture and release, which could be used to build noninvasively removed bioelectronic devices or cell-detachable sensors. Combining the molecular design features, these materials further demonstrated the following superior material performances. They displayed low impedance at low frequency range, and this is ideal for biological application. The biofunctions presented on the surface could also provide biochemical stimulation to the differentiation of cells attached. Stable binding and electrical communication could be created between biomimetic PEDOTs and cells attached. The cell-substrate interaction could be largely enhanced by Nano-assemble of PEDOTs or extension of polymer chains on surfaces.

V.6..2
09:10
Authors : Jouni Ahopelto
Affiliations : VTT Technical Research Centre of Finland, Microsystems and Nanoelectronics, jouni.ahopelto@vtt.fi

Resume : Preventive health care is an emerging trend requiring easy to use methods and tools. Health monitoring on daily or weekly basis would benefit from fast and label-free detection of for example saliva, urine or blood, and from portable units with connectivity via internet or mobile phones. Most of the biomolecules carry net charge detectable with charge sensitive devices, such as field effect transistors, leaving the problem to achieve selectivity and immobilisation of the receptors. Hydrophobins are protein amphiphiles with a hydrophobic patch at one end facilitating formation of well-ordered monolayers on hydrophobic surfaces and, consequently, providing means to immobilize receptors [1]. Hydrophobins have been used to exfoliate and in-situ functionalise graphene flakes [2] and for directed self-assembly of gold nanoparticles on silicon surfaces [3]. Hydrophobins can be genetically engineered to realise receptor modules specific to various analytes. In these experiments graphene field effect transistors (GFET) have been used as charge detectors and the non-linear characteristics near the Dirac point provide very high charge sensitivity. The fusion proteins form dense monolayer on the hydrophobic graphene channel [4] and immobilise the receptors enabling selective bio-recognition. We have tested the approach with hydrophobin-ZE - ZR amino acid zipper pair and hydrophobin-protein A – IgG immunoglobulin antibody and measured sensitivities at the level of femtomols with a few seconds response time. In the presentation we will discuss the possible applications and the potential limitations of the concept. [1] Linder, M. B., Szilvay, G. R.,Nakari-Setälä, T., Penttilä, M. E., FEMS Microbiology Reviews 29 (2005) 877–896. [2] Laaksonen, P., Kainlauri, M., Laaksonen, T., Shchepetov, A., Jiang, H., Ahopelto, J., and Linder, M. B., Angew. Chem. Int. Ed., 49, (2010), 4946-4949. [3] Laaksonen, P., Kivioja, J., Paananen, A., Kainlauri, M., Kontturi, K., Ahopelto, J., and Linder, M.B., Langmuir 25 (2009) 5185–5192. [4] Kivioja, J.; Kurppa, K.; Kainlauri, M.; Linder, M. B.; Ahopelto, J., Appl. Phys. Lett. 94 (2009) 183901.

V.6..3
09:40
Authors : Koichi Kato (kokato@hiroshima-u.ac.jp), Shintaro Sakakitani, Chiharu Kubota, Ryo Nishikiori
Affiliations : Department of Biomaterials, Institute of Biomedical & Health Sciences, Hiroshima University

Resume : It may be expected that methods for investigating cell-cell interactions in an in vitro tissue model will facilitate to gain deeper insights into operating principles underlying organogenesis during development and tissue regeneration. Here we present our approach in which a cell culture substrate was fabricated through micrometer-scale two-dimensional antibody display for permitting to investigate dynamics of heterotypic cellular interactions. In this study, special attention was paid to an epithelial-mesenchymal interaction that is ubiquitously seen in tissue morphogenesis. For the preparation of a cell culture substrate, the surface of a glass plate was cationically modified and then used as a substrate for two-dimensional antibody display. Using a poly(dimethylsiloxane) stamp with a microfluidics, two antibodies against distinct surface markers of epithelial and mesenchymal cells were immobilized in a site-addressable manner: Two antibodies were immobilized in separate stripes in close proximity to each other. To demonstrate the feasibility of the substrate for micrometer-scale positioning of heterotypic cells, populations of epithelial and mesenchymal cells were simultaneously seeded to the substrate that displayed antibodies. It was shown that our method using the stamp with a microfluidics allowed us to immobilize two different antibodies in a site-addressable manner. On this substrate, gingival epithelial cells and mesenchymal cells could be successfully positioned to the regions where antibodies specific for either of the two cell types were immobilized. These cells could be further cultured in situ on the substrate for analyzing cellular proliferation, migration and morphological changes. The results obtained in our study suggest that the two-dimensional antibody display serves to establish the spatially-controlled co-culture of two different cell populations, providing an initial condition for further study on the dynamic evolution of heterotypic cellular systems.

V.6..4
10:10
Authors : Shigeori Takenaka
Affiliations : Department of Applied Chemistry & Research Center for Bio-microsensing Technology, Kyushu Institute of Technology; shige@che.kyutech.ac.jp

Resume : To understand life it is important to clarify various phenomena that occur in the cell at the molecular level. Several methods have been developed to monitor cellular processes, especially, imaging of specific signaling molecules including metal ions that may provide spatiotemporal information on their location in the cell. Since potassium ion (K+) plays an important role in many physiological events such as homeostasis in the heart muscle and hyper polarization of neurons, it is important to develop not only a detection method for this cation, but also a fluorescence imaging technique. However, specific detection of K+ is hampered because of the existence of sodium (Na+) and other ions in the cell. Oligonucleotides with sequences of human telomeric DNA or thrombin binding aptamer (TBA) are known to form tetraplex structures upon K+ ion binding. We successfully synthesized a novel fluorescent reagent, PSO-5, showing preference for K+ on the basis of a conformational change in G-rich DNA. PSO-5 consisted of thrombin binding aptamer (TBA) carrying FAM at the 5’-end conjugated with a peptide carrying TAMRA and biotin at the middle and at its C-terminus, respectively. The ternary complex of streptavidin with PSO-5 and biotinylated nuclear export signal peptide in a 1:1:3 stoichiometry had the dissociation constant of 2.24 mM for K+ and its preference for K+ is 236 times over Na+. K+ in the cell was visualized based on the FRET ratio of this complex. Changes in the FRET signal of PSO-5 was observed to indicate a decrease in K+ concentration in the cell upon addition of amphotericin B and ouabain which facilitate K+ efflux from the cell.1 [1] K. Ohtsuka, S. Sato, Y. Sato, K. Sota, S. Ohzawa, T. Matsuda, K. Takemoto, N. Takamune, B. Juskowiak, T. Nagai, S. Takenaka, Chem. Comm., 2012, 48, 4740-4742.

V.6..5
11:00
Authors : Shutao WANG
Affiliations : Technical Institute of Physics and Chemistry of the Chinese Academy of Sciences; stwang@mail.ipc.ac.cn

Resume : Circulating tumor cells (CTCs) have become an emerging ?biomarker? for monitoring cancer metastasis and prognosis. Although there are existing technologies available for isolating/counting CTCs, the most common of which using immunomagnetic beads, they are limited by their low capture efficiencies and low specificities. By introducing a three-dimensional (3D) nanostructured substrate ? specifically, a silicon-nanowire (SiNW) array coated with anti-EpCAM ? we can capture CTCs with much higher efficiency and specificity. The conventional methods of isolating CTCs depend on biomolecular recognitions, such as antigen-antibody interaction. Unlikely, we here proposed that nanoscaled local topographic interactions besides biomolecular recognitions inspired by natural immuno-recognizing system. This cooperative effect of physical and chemical issues between CTCs and substrate leads to increased binding of CTCs, which significantly enhance capture efficiency. Recently, we have also developed a 3D cell capture/release system triggered by aptamer enzyme, electrical potential and Temperature, which is effective and of ?free damage" to capture and release cancer cells. The bio-inspired interfaces of cell capture and release open up a light to rare-cell based diagnostics, such as CTCs, fetal cells, stem cell and so on.

V.6..6
11:50
Authors : G. Rosenman, A. Handelman
Affiliations : Tel Aviv University: Holon Institute of Technology; gilr@eng.tau.ac.il

Resume : Genetically encoded green fluorescent protein and its homologs opened the avenue for a wide applications of these intrinsic biological labels representing specific biological proteins. In this work we report on a new visible blue/green fluorescent phenomenon found in -sheet nanowires of ultrashort bionspired peptides. Di- and tri-peptide nanostructures self-assembled from biomolecules of different compositions and origin such as aromatic, aliphatic, linear and cyclic (diphenylalanine, FF-; dileucine, LL-; triphenylalanine, FFF- monomers) have been studied. At elevated temperature 140-180?C these supramolecular structures of diverse morphologies undergone the same irreversible thermally-induced phase transformation. This reconstruction process is followed by deep modification at all levels: molecular, electronic, elementary symmetry, peptide secondary structure, displaying new nanowire morphology and completely new common physical properties. The thermally-induced nanowire supramolecular ensembles acquire -sheet secondary structure with new building blocks and new non-covalent hydrogen bonds. In this phase the -sheet nanowires, irrespective of their native biomolecules origin, exhibit similar profound alteration of optoelectronic properties and appearance of visible (blue and green) fluorescence ascribed to non-covalent hydrogen bonds. Observed visible fluorescent of self-assembled dyes (hydrogen bonds) in thermally-induced peptide fiber nanostructures can be used as intrinsic optical labels in biomedicine as well for a new generation of novel optoelectronic biocompatible nanomaterials for emerging nanophotonic applications such as bio-lasers, integrated optics and biocompatible markers.

V.6..8
12:05
Authors : Arzum Erdem
Affiliations : Ege University, Faculty of Pharmacy, Analytical Chemistry Department, 35100 Bornova, Izmir, TURKEY arzum.erdem@ege.edu.tr

Resume : Screen-printed electrodes (SPEs) are used as cost-effective (bio)sensor platforms, which have been successfully utilised for rapid in situ analysis of biomarkers, enviromental pollutants, biorecognition interactions such as nucleic acids, or proteins due to their advantageous material properties, disposability, simplicity, and rapid responses (1-10). Screen printed electrodes have been fabricated as the miniaturized and multi channel forms of the electrochemical analysis systems (8-10). These disposable (bio)sensors can easily be modified with different nanomaterials such as carbon nanotubes, nanoparticles and dendrimers [4,7,10]. They are appropriate candidates for on-line measurements of numerous biological samples due to require low sample volumes. Multi channel screen-printed electrochemical array systems tested for biomolecular interactions have been overviewed herein for monitoring of spesific biomolecular recognitions; such as, nucleic acids and aptamer-protein interactions with their advantages and further applications. Acknowledgements. A.E would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as the associate member of TUBA for its partial support. References: 1- J. Wang, Electroanalysis 17, 2005, 7-14. 2- M. Li, Y.-T. Li, D.-W. Li, Y.-T. Long, Analytica Chimica Acta, 734, 2012, 31?44 3- O. Dom?nguez Renedo,M.A. Alonso-Lomillo, M.J. Arcos Mart?nez, Talanta 73, 2007, 202?219. 4- D. Kumar, B.B. Prasad, Sensors and Actuators B: Chem. , 171, 2012, 1141-1150. 5- J. Wang, D. Xu, A. Erdem, R. Polsky, M. Salazar, Talanta, 56, 2002, 931-938 6- J. Wang, J.-W. Mo, A. Erdem, Electroanalysis, 14, 2002, 1365-1368. 7- F. Rohrbach, H. Karadeniz, A. Erdem, M. Famulok, G. Mayer, Analytical Biochemistry, 421, 2012, 454-459. 8- A. Erdem, G. Congur, E. Eksin, Sensors and Actuators B: Chem., 188, 2013, 1089-1095. 9- A. Erdem, G. Congur, Talanta, 118, 2014, 7-13. 10- A. Erdem, G. Congur, Sensors and Actuators B: Chem., 196, 2014, 168-174.

V.6..9
12:30
Authors : Jau-Ye Shiu, Viola Vogel
Affiliations : Jau-Ye Shiu, shiuj@mat.ethz.ch

Resume : Cells are known to probe different physical properties of their environment. In the mechanotransduction field many tools are used to study these signaling events, such as engineered substrates that allow cellular traction forces measurements. Our study introduces a novel nanopillar platform, which revealed that the forces acting on perinuclear nanopillars are rich in α5β1-integrins and significantly higher than those acting on αvβ3 integrin-rich peripheral focal adhesions. These perinulear forces increased upon integrin activation using Mn2 and decreased with pharmacological Latrunculin B* treatement. LMNA knockouts allowed to manipulate peripheral versus perinuclear traction forces and confirmed that these central forces originate from actin fibers that span across the cell nucleus.

V.6..10

No abstract for this day


Symposium organizers
Contact us: the symposium’s Working Team emrs@univ.kiev.ua
Karsten HauptCompiegne University of Technology, CNRS / Institute for Enzyme and Cell Engineering Biomimetic Polymers / Molecular Imprinting and Nanostructuration Group

Rue du Dr. Schweitzer F-60200 Compiegne France

+33 344 23 44 08
+33 344 23 44 08
karsten.haupt@utc.fr
Peter SCHARFFTechnical University of llmenau

Institute of Chemistry and Biotechnology, Weimarer Strasse 25, 98693 Ilmenau, Germany

+49 36 77 69 3603(04)
peter.scharff@tu-ilmenau.de
Michael H. BartlDep. of Chemistry / Physical and Materials Chemistry Group

315 South 1400 East, Rm 4402 Salt Lake City, UT 84112-0850 USA

(801) 585 5160
(801) 581 8433
bartl@chem.utah.edu
Eugenia BUZANEVATaras Shevchenko National University of Kyiv

NASU “Physical and Chemical Material Science Centre”, Volodymyrs'ka Str. 64/13, 01601 Kyiv, Ukraine

+38 044 294 26 22
emrs@univ.kiev.ua