preview all symposia

Biomaterials and soft materials


Bioinspired and biointegrated materials as new frontiers nanomaterials

This symposium is composed of SPECIAL SESSIONS covering hot topics such as biomimetic materials, supramolecular nanomaterials and smart materials. These materials find applications in a range of fields encompassing smart living building and human health.


The newest research ideas and nanotechnologies from smart (nano)materials, integrated systems to robotic devices will be presented. The covered themes include tissue and organ regeneration (e.g. skin, bone tissue and neural networks) as well as implantable bionic systems.

These systems can include, but is not limited to, molecular systems, nanomaterials (templated by biomolecules such as viruses, marine plants, proteins, pigments), nanoparticles as nanorobots for in vivo applications, supramolecular complexes, inorganic NPs.

The symposium will bring together researchers from chemical, physical sciences and bio - science and – nanotechnology biomaterials for nanomedicine and engineering bio - electronic, - photonic, - magnetic nanosystems to discuss the latest advancements.

Proposed subjects for discussions at this event have actuality for Investigators - Collaborators of the EU COST Actions, as and the EU HORIZON 2020-2025 Projects.

A special Young Scientist FORUM (hosting Post-Graduate, PhD and Graduate students’ talks) will be held at the symposium’s second day.

Hot topics to be covered by the symposium:

  1. Design and synthesis of (supramolecular) materials and their action in  bio – mimetic material synthesis;
  2. Bioinspired inorganic, smart nanoparticles (NPs);
  3. Design and creation of smart supramolecular materials and their biohybrids;
  4. Novel concepts in nano - characterization, bio-recognition of smart and specially bio-hybrid stimuli responsive nanomaterials with applications for clinical, food and environmental monitoring;
  5. Molecularly imprinted materials;
  6. Smart, biomimetic materials as scaffolds for tissue engineering;
  7. Electronic/photonic/magnetic smart biomolecules (nucleic acids, virus, marine plants proteins, pigments) and their mimetic analogues;
  8. Neuroelectronics;
  9. Biosensing devices for medical (in vivo and in vitro diagnostics) and environmental biotechnologies: developed 3rd generation biosensors, carbon 2D materials, bioelectronic textiles, printed paper electronics, electronic skin);

Invited partners:



272.27 KbDownload
Start atSubject View AllNum.Add
08:50 Opening remarks & Symposium Presentation Prof. Insung S. CHOI    
Smart Nanomaterials and Nanosystems Strategy. Forum Key Presenters : Chairs - Dr.Emmanuel I.Stratakis (Institute of Electronic Structure and Lasers -FRT Hellas,Greece), Professors: Insung S. Choi (KAIST,Korea), Bert Mueller (University of Basel, Switzerland),Yutaka Wakayama (National Institute for Materials Science, Japan), Joerg K.N.Linder ( Universitat Paderborn, Germany), Roger Woerdenweber (Universitat zu Koeln, Germany)
Authors : Emmanuel Stratakis1,2
Affiliations : 1Foundation for Research and Technology - Hellas (F.O.R.T.H.), Institute of Electronic Structure and Laser (I.E.S.L.), Heraklion, Crete, Greece, 2 Physics Department, University of Crete, Heraklion, Crete, Greece

Resume : The surface topography of biomaterials can have an important impact on the cellular adhesion, growth and proliferation. Apart from the overall roughness, the detailed morphological features at all length scales, significantly affect the cell-biomaterial interactions in a plethora of applications including structural implants, tissue engineering scaffolds and biosensors. We present simple, one-step direct femtosecond (fs) laser patterning techniques to fabricate various types of micro/nano structured biomaterials platforms. Variation of the laser fluence, alters the surface morphology of solid materials, leading to a rippled-type, at lower laser fluences, or a conical spiked-type morphology, as the laser fluence increases. Hierarchically-structured cell culture platforms incorporating gold nanoparticles functionalized with specific bio-functional moieties have been additionally developed. Cells with nerve cell phenotype were cultured on the substrates. More specifically, PC12 cells cultured on the developed substrates and treated with nerve growth factor showed a differentiation response that was highly dependent on the surface topography. While, experiments with DRG/SCG nerve cells showed a differential orientation of the cells, depending of the underlying geometry of the laser engineered surface structures (Figure 1). Depending on the laser processing conditions, distinct SW10 cell-philic or cell-repellent patterned areas can be attained with a desired motif, enabling spatial patterning of cells in a controllable manner. Furthermore, we report on the fs laser microfabrication of porous collagen scaffolds, providing implants that via precise micron-sized features held the promise to control neuron cell phenotypes. Finally, the fs laser fabrication of a novel microfluidic platform for the study of the combined effect of fluid shear forces and culture substrate morphology on neuron cell proliferation and directionality will be demonstrated. Our work provides a versatile laser-based biofabrication approach to tune neuron cell responses by proper selection of the surface free energy of the substrate and may be promising for the design of cell culture biomaterial platforms for neural tissue engineering applications.

Authors : Karsten Haupt; Paulina Medina Rangel; Jingjing Xu; Bernadette Tse Sum Bui
Affiliations : Sorbonne Universities, Université de Technologie de Compiègne, CNRS Institute for Enzyme and Cell Engineering, Compiègne, France

Resume : Molecularly imprinted polymers (MIPs) are synthetic antibody mimics that specifically recognize molecular targets.1,2 They are highly cross-linked polymers synthesized in the presence of the target molecule ore an epitope thereof, acting as a molecular template. This templating induces three-dimensional binding sites in the polymer that are complementary to the template in size, shape and chemical functionality. The synthetic antibody can recognize and bind its target with an affinity and selectivity similar to a biological antibody.1 Herein, we demonstrate the potential of MIPs for antibody therapy on the example of two cell surface biomarker targets, glycans and a cell-cell adhesion protein.3 We also present the potential use of MIPs for the inhibition of cell-virus interactions.4 Finally, their applications in diagnostics and bioanalysis will be demonstrated.3,5

Authors : Marleine Tamer (a,b), Maria Bassil (b), Mario El Tahchi (b), Sebastien Balme (a), Philippe Miele (a), and Mikhael Bechelany (a)
Affiliations : a. European Institute of Membranes, ENSCM, CNRS, University of Montpellier, France.; b. Laboratory of Biomaterials and Intelligent Materials, Department of Physics, Lebanese University - Faculty of Science II, Jdeidet, Lebanon.

Resume : Organs-on-a-chip technology has shown strong promise in organ replication, combining bioengineering and microfluidic technologies to closely mimic the natural organ [1,2]. Kidney-on-a-chip has emerged as a novel strategy for the improvement of filtration function in the case of kidney disease [3]. Inspired by the natural organ and the dialysis system, we designed a new bioartificial kidney device composed of several filtration units. Each unit encapsulates a Polyacrylamide hydrogel matrix, considered as functional unit of the device and acts as an exchange membrane. Several hydrogel matrices are prepared and their physicochemical properties are tailored as desired in order to test their effects on the filtration ability and efficiency of the “filtration unit”. Results show that the speed of solute diffusion across the matrix is controlled by varying the pores number, while the size selectivity and the molecular weight cutoff of the matrix depend on the pore size. Bovine serum albumin 66 kDa and Fluorescein isothiocyanate–dextran (MW: 40, 70, 150 kDa) were used to test the molecular weight cutoff of PAAM membrane as they mimic the blood proteins. The results show a restriction of the passage of proteins having a molecular weight above 66 kDa across the membrane. The ion selectivity of the membrane was tested to verify the stability of a normal body fluid composition. The future bioartificial kidney would be an assembly of several filtration units working in parallel each having a specific function and imitating a specific renal activity. 1. Zheng, F. et al. Organ-on-a-Chip Systems: Microengineering to Biomimic Living Systems. Small 12, 2253–2282 (2016). 2. Ashammakhi, N., Wesseling-Perry, K., Hasan, A., Elkhammas, E. & Zhang, Y. S. Kidney-on-a-chip: untapped opportunities. Kidney Int. 94, 1073–1086 (2018). 3. Wilmer, M. J. et al. Kidney-on-a-Chip Technology for Drug-Induced Nephrotoxicity Screening. Trends Biotechnol. 34, 156–170 (2016).

Round Table Discussion : invited 7en minutes Key Speech on Frontier Reserch
Authors : Thomas J. Webster, Art Zafiropoulo Chair
Affiliations : Department of Chemical Engineering, Northeastern University, Boston, MA 02115

Resume : There is an acute shortage of organs due to disease, trauma, congenital defects, and most importantly, age related maladies. While biotechnology (and nanotechnology) has made great strides towards improving tissue growth, infection control has been largely forgotten. Critically, as a consequence, the Centers for Disease Control have predicted more deaths from antibiotic-resistant bacteria than all cancers combined by 2050. Moreover, there has been a lack of biotechnology translation to real commercial products. This talk will summarize how nanotechnology with FDA approval can be used to increase tissue growth and decrease implant infection without using antibiotics. Studies will also be highlighted using nano sensors (while getting regulatory approval). Our group has shown that nanofeatures, nano-modifications, nanoparticles, and most importantly, nanosensors can reduce bacterial growth without using antibiotics. This talk will summarize biotechnology techniques and efforts to create nanosensors for a wide range of medical and tissue engineering applications, particularly those that have received FDA approval and are currently being implanted in humans.

Authors : Monica Marini1, S. Stassi,1 M. Allione,2 B. Torre,2 A. Giugni,2 M. Moretti,2 C.F. Pirri,1 C. Ricciardi,1 E. Di Fabrizio2
Affiliations : 1 Department of Applied Science and Technology (DISAT), Polytechnic of Turin, Italy 2 SMILEs Lab, PSE Division, KAUST, Thuwal 23955, Saudi Arabia

Resume : Super-hydrophobic surfaces (SHS) with regular patterns of micropillars were used to suspend DNA molecules and DNA after its interaction with other compounds such as protein, intercalants, and chemotherapeutic drugs. Briefly, a 5l droplet of a physiological-compatible solution containing nucleic acids and ligands is pipetted on the SHS. At temperature and humidity-controlled conditions the solution evaporates, and the droplet reduces the volume retracting from one pillar to the following one. The DNA helices follow this movement and the molecules linked to the top of one pillar are pulled to the next in line. With this technique we obtained free-standing self-assembled back ground-free DNA bundles, analysed by several techniques such as Raman Spectroscopy, high-resolution transmission electron microscopy (HRTEM), and Laser Doppler Vibrometer. The strategy shown allows studying the native characteristics of DNA helices and their alterations to the pristine conditions, showing a great potential in medical-oriented research.

Authors : Ch. Wenger
Affiliations : IHP - Leibniz Institut fuer innovative Mikroelektronik, 15236 Frankfurt / Oder, Germany

Resume : Recently, artificial intelligence reached impressive milestones in many machine learning tasks such as the recognition of faces, objects, and speech. These achievements have been mostly demonstrated in software running on high-performance computers. Novel CMOS approaches with in-memory processing is however more promising in view of the reduced latency and the improved energy efficiency. In this scenario, emerging memory technologies as resistive switching memory (RRAM), have been proposed for hardware accelerators of both learning and inference tasks. Latest results support RRAM technology as memristive synapses for in-memory hardware accelerators of machine learning.

Authors : Valerio Voliani
Affiliations : Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, P.zza San Silvestro, 12 - 56127, Pisa (PI), Italy.

Resume : Inorganic nanomaterials hold the promise to shift the current medical paradigms for the treatment and diagnosis of several diseases, among which neoplasms and infections. This is due to their peculiar chemical, physical and physiological behaviors. Despite the massive efforts, treatments based on inorganic nanomaterials are mainly at the preclinical stage, due to the body persistence issue. Indeed, non-biodegradable materials usually result in long-term persistence within excretion system organs, increasing their likelihood of toxicity. In this round table, the next strategies to support the translation of inorganic nanomaterials to the clinical settings will be discussed. The research leading to these results has received funding from AIRC under MFAG 2017 – ID 19852 project – P.I. Voliani Valerio.

Start atSubject View AllNum.Add
08:45 Plenary Session (08. 45 – 09.45): Lecture by Professor Sir James Fraser Stoddart, Nobel Laureate in Chemistry (2016), Northwestern University, USA    
09:45 The Nobel Prize in Chemistry 2016 was awarded jointly to Jean-Pierre Sauvage, Sir J. Fraser Stoddart and Bernard L.Feringa “for the design and synthesis of molecular machines”    
09:45 10.00 - Coffee Break    
Young Investigator Forum : Invited Organizers/Chairs - PhD, Post-Doctoral Researchers Nanasaheb D.Thorat (Poland)and Katharina Brassat (Germany), PhD student Toshifumi Imajo (Japan)
Authors : Professor Insung S.Choi
Affiliations : KAIST, Korea

Resume : 2020 Young Investigator Forum - 107 Presentations on Frontier Research in Smart Nanomaterias, Nanosytems for a better life building and Human well-being

SESSION Frontier Research in Theranostics : Invited Organizer/Chair - PhD, Post-Doctoral Researchers Nanasaheb D.Thorat (Poland)
Authors : Nanasaheb D. Thorat, Joanna Bauer
Affiliations : Department of Biomedical Engineering, Faculty of Fundamental Problems in Technology, Politechnika Wroclawska, Wroclaw, Poland

Resume : Countless efforts are made by scientist to design different type of nanostructures, in the investigation for next generation cancer care applications. Various nanostructures often employ multimodality and show encouraging results at preclinical stage in cancer therapy. Specially designed smart nanostructures such as hybrid nanostructures are responsive to external physical stimuli such as light, magnetic field, electric, ultrasound, radio frequency, X-ray, etc are currently being investigated for cancer therapy. Thus, hybrid nanostructures provide a unique combination of important properties to address key challenges in modern cancer therapy: (i) an active tumor targeting mechanism of therapeutic drugs driven by a physical force rather than passive antibody matching, (ii) an externally/remotely controlled drugs on-demand release mechanism, and (iii) a capability for advanced image guided tumor therapy and therapy monitoring. The present report/talk cover various type of nanostructures that are developed by our group and those are responsive to magnetic and light-triggered modalities currently being developed for nonconventional cancer treatments. The physical basis of nanostructures and stimuli responsive modality is explained; so audience with a physics or, materials science background can easily grasp new developments in this field.

Authors : Grace Brennan1, Nanasaheb Thorat1,2, Martina Pescio1, Silvia Bergamino1, Joanna Bauer2, Ning Liu1, Tofail Syed1, Christophe Silien1.
Affiliations : 1 Modelling Simulation and Innovative Characterisation (MOSAIC), Department of Physics, School of Natural Sciences and Bernal Institute, University of Limerick, Limerick, V94 T9PX, Ireland. 2 Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology, Wybrzeże Stanisława Wyspiańskiego 27, Wrocław 50-370, Poland.

Resume : Magnetic-plasmonic, Fe3O4-Au nanoparticles have been of interest in recent years, owing to their bifunctionality and biocompatibility. A plasmonic gold shell facilitates enhanced optical properties, with strong light scattering and absorption capabilities, facilitating bioimaging, surface-enhanced Raman and photothermal activity. Furthermore, thanks to its magnetic core, magnetic manipulation can be achieved which can be exploited in magnetic-field directed drug delivery, MRI contrast imaging and alternating magnetic-field hyperthermia. With great potential for clinical translation, it is important to uncover the physical properties these particles possess, which may help to optimise the stimuli applied to achieve the most efficient theranostics. Herein, superparamagnetic cores are functionalised with varying stages of gold growth from core-satellite structure to full gold shell growth. The optical extinction and single-particle scattering spectra of the various nanostructures are collected, exposing a spectral drift (up to 110 nm) or difference between the extinction and scattering maxima wavelengths. We explore how such a large drift may be of use in multimodal nanotheranostics, where photothermal therapy is conducted near the maximum absorption wavelength and imaging is carried out near the scattering maximum. COMSOL Multiphysics ® was used to create finite element analysis (FEA) models in wave optics and heat transfer to support the experimental findings. We also explore the effects of the gold shell on the magnetic properties of the multistage nanoparticles. This work was supported by Science Foundation Ireland (SFI) centre CÚRAM, the European Regional Development Fund (Grant Number 13/RC/2073), Marie Sklodowska-Curie Grant Agreement No. 751903. and CDA award 13CDA2221.

Authors : Erik Laurini, Domenico Marson, Suzana Aulic, Andrea Mio, Maurizio Fermeglia and Sabrina Pricl
Affiliations : MolBNL@UniTS Laboratory, Department of Engineering and Architecture, University of Trieste, Trieste, 34018, Italy

Resume : Theranostics is a new field of medicine which combines specific targeted therapies based on specific targeted diagnostic tests. The theranostic paradigm in cancer involves nanoscience to unite diagnostic and therapeutic applications to form agents for diagnosis, drug/gene delivery and treatment response monitoring. In this respect, our team as a part of a pan-European task force in the field has developed a series of innovative theranostic systems which proven to be excellent agents in cancer imaging and therapeutics in vivo. Nanotechnology-based imaging in cancer diagnosis plays a prominent role in both improving imaging sensitivity and specificity and reducing toxicity. Quite recently, based on the previous experience with Amphiphilic Dendrimers (AD) and further studies on AD modifications, we developed innovative nanosystems for positron emission tomography (PET) and single photon emission computed tomography (SPECT) imaging again exploiting AD-based self-assembly in which the surface of the amphiphile was decorated with different radionuclide (Gallium, Gadolinium and Indium) complexed within different macrocyclic chelator. The key findings of these efforts can be summarized as follows: the nanovectors were characterized by effective accumulation in tumors, exceeding sensitivity and specific imaging of various tumours, and was especially efficacious for tumors otherwise undetectable using the clinical gold reference. In addition, this nanovectors were endowed with an excellent safety profile and favorable pharmacokinetics. This study also demonstrated that nanotechnology based on self-assembling dendrimers can a fresh perspective for biomedical imaging and cancer diagnosis, i.e., cancer theranostics.

Authors : Dr. Ganeshlenin Kandasamy
Affiliations : Department of Biomedical Engineering, Vel Tech Rangarajan Dr. Sagunthala R&D Institute of Science and Technology, Chennai, Tamil Nadu, India

Resume : The induction of the heat by the magnetic nanoparticles (especially superparamagnetic iron oxide nanoparticles – i.e., SPIONs (i.e., Fe3O4/Fe2O3)) under the exposure of an alternating magnetic field (AMF) has a remarkable potential for being utilized in distinct non-invasive cancer nanotherapeutics such as magnetic hyperthermia therapy (or thermotherapy) and magnetically-triggered release of drugs for chemotherapy. But, the poor induction efficacies (estimated using parameters such as specific absorption rate (SAR) and/or intrinsic loss power (ILP)) of these SPIONs in the controlled environments have hampered their practical implementation so far. This could be mainly attributed to the significant lack of any of the following properties of the SPIONs: suitable size/shape, uniform aqueous dispersibility, biocompatibility, saturation magnetization and/or surface chemistry (for cell uptake/ functionalization). Therefore, it is highly desirable to prepare the SPIONs with the improved physicochemical, magnetic and biological properties for efficient nanotherapeutics. We have approached this via two different processes. In the first process, we have surface-engineered (individual or combined) (i) the unicore hydrophilic SPIONs via π-π conjugated carboxylic acid (COOH-)/amine (NH2-) based surfactants, and (ii) the multicore hydrophilic SPIONs (also called as iron oxide nanoclusters - IONCs) via ethylene glycol & ethanolamine based solvents in in situ manner through co-precipitation and/or thermolysis synthesis methods. It has been observed that these unicore/multicore SPIONs have possessed better properties including colloidal stability, and showed better efficacies (i.e., heat induction (SAR/ILP) & therapeutic) in the treatment of breast and liver cancers via thermotherapy. In the second process, we have encapsulated the as-synthesized hydrophobic SPIONs inside polymeric nanoparticles along with two diverse drugs – i.e., curcumin (a chemotherapeutic drug) and verapamil/nifedipine (calcium channel blocker (CCB) based drugs) to form multi-functional nanoparticles. The polymeric nanoparticles are made of poly(lactic-co-glycolic acid) (PLGA) in association with different stabilizers (i.e., polyvinyl alcohol (PVA) or d-α-tocopherol polyethylene glycol 1000 succinate (TPGS – vitamin E molecules)). Herein, the in vitro magnetically-triggered drug release studies have revealed that the application of AMF has effectually enhanced the discharge of the drugs, which have consequently improved the growth inhibition rates in the cervical and liver cancer cells via combined thermotherapy and chemotherapy (i.e., multimodal cancer nanotherapeutics).

Authors : Vijaykumar V. Jadhav, Pritamkumar V. Shinde, Rajaram S. Mane, and Colm O’Dwyer
Affiliations : School of Chemistry, University College of Cork, Cork, Ireland Department of Physics, Shivaji Mahavidyalaya, Udgir, India Center of Nanomaterials for Energy Harvesting/Generation and Storage Technologies, School of Physical Science, S. R. T. M. University, Nanded, Maharashtra, India

Resume : Hybrid nanostructures of metal, polymer, chalcogenide, oxide, hydroxide, nitride, and carbonaceous materials are believed to promise a great impact on the next generation of cancer diagnosis and therapy technologies. This chapter focuses on hybrid nanostructures that contain two or more distinct nanoparticles assembled in a functional structure that itself is still of nanoscale dimensions. This type of research aims to build a hybrid nanostructure whose medical effects are superior to those that could be realized from any simple mixture of the individual nanostructures. The unique characteristics of hybrid nanostructures in the nanometer range, such as high surface-area-to-volume ratio or shape/size-dependent optical properties, are drastically different from those of their bulk counterparts and hold pledge in the clinical field for disease therapeutics. This work deals with various kinds of novel hybrid nanostructures such as TiO2@Au, nanotube, silica, gold, and polymer that have demonstrated potential biogenic applications, especially in cancer research, including detection and treatments. Synergistic effects corroborated in these hybrid organic and inorganic nanostructures, useful for theranostics, are also explored in this article.

Authors : Niroj Kumar Sahu
Affiliations : Associate Professor, Centre for Nanotechnology Research, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India

Resume : Nanoparticle mediated chemotherapy for cancer is a potentially curative treatment modality that has been undergoing rapid technological improvements with the development of nanotechnology. Since chemotherapy is not fully effective at certain therapy resistance tumor cells, a combination of drug and heat can effectively exhibit a tumoricidal effect assuring selective attachment to target sites conserving surrounding healthy tissues. Nanomaterials are potential candidates to get functionalized for both chemotherapy and thermal therapy (hyperthermia) in a single system. Further, the dual drug delivery system using two drugs shows effective synergistic effects which enhances tumor regression capabilities compared to individual analog. In this regard, formulation of nano drug delivery systems by layer-by-layer (LBL) techniques employing dual drug loaded magnetite (Fe3O4) nanoparticles for synergistic chemothermal therapy has been investigated. The multifunctional mesoporous dual drug delivery system having magnetite with spherical morphology and high drug loading capacity shows sustained release in lysosomal and physiological pH. Polyphenolic drug increases the effect of DOX in MDA-MB-231 cells as well as repair the DOX induced cardiotoxicity. Thus this nano drug delivery system can be efficiently utilized for thermo-chemotherapy of cancer as well as to combat the cardiotoxicity induced by DOX which were investigated in vitro in H9C2 cells.

Authors : Enrico Catalano, Jürgen Geisler , Vessela N. Kristensen
Affiliations : Faculty of Medicine, University of Oslo (UiO), Oslo, Norway

Resume : Breast cancer is the second leading cause of death in women worldwide. The extremely fast development of metastasis and ability to develop resistance mechanism to all the conventional drugs make them very difficult to treat which are the causes of high morbidity and mortality of breast cancer patients. Nanotheranostics can be used for this purpose to implement the integration of diagnostic and therapeutic function in one system using the benefits of nanotechnology, with the purpose to treat cancer like one-size-fits-all scenario. The development of liposomes conjugated to superparamagnetic gold-coated iron oxide nanoparticles (Au-SPIONs) functionalized with an aptamer, to mimic natural antibody targeting, was implemented to allow target delivery of the anticancer drug of doxorubicin (DOX) on triple-negative breast cancer cells. Liposomes with iron oxide nanoparticles (SPIONs) and gold nanoparticles (GNPs) were prepared by ultrasound-assisted and controlled seeded growth synthetic methods, respectively. The aptamers AS1411, ERaptD4 and H2 were loaded by a desolvation cross-linking method and characterized by magnetic and physicochemical techniques. The synthesized nanoparticles were found to be spherical with an average diameter of 90 nm and zeta potential of about -49.4 mV. The level of cell death involved in the pathway of apoptosis, were measured to evaluate the synergistic effect of Au-SPIONs-mediated RF hyperthermia. MCF-7 and MDA-MB-231 human breast cancer cells were treated with different concentrations of Au-SPIONs. After incubation with NPs, the cells were exposed to RF waves (13.56 MHz; 100 W; 15 min). Cellular uptake of nanoparticles was confirmed qualitatively and quantitatively. The in-vitro anti-tumor effect of the designed delivery vehicle on MCF7 and MDA-MB-231 human breast cancer cells was evaluated by cell viability assay. The results obtained from cell viability and apoptosis evaluation showed that NPs and radiofrequency (RF) had no significant effect when applied separately, while their combination had synergistic effects on cell viability percentage and doxorubicin release and the level of apoptosis induction. The experimental results revealed that it could significantly inhibit the proliferation of cancerous cells. Aptamer-functionalized magnetic liposomes improved cellular uptake and efficiency to breast cancer cells as compared to non-targeting nanoparticles because of the high affinity of mentioned aptamer toward the overexpressed nucleolin and targeting of HER2 and ERα on MCF7 and MDA-MB-231 cell surface. The obtained results showed that the use of aptamer-functionalized magneto-plasmonic NPs in the process of hyperthermia and radiofrequency (RF) of cancer holds a great promise to develop a new combinatorial breast cancer therapy strategy.

Authors : Vishwajeet M Khot
Affiliations : Department of Medical Physics, Center for Interdisciplinary Research, D Y Patil Education Society (Institution Deemed to be University), Kolhapur-416006 (MS) India

Resume : Magnetic Hyperthermia Therapy (MHT) with magnetic nanoparticles (MNPs) has emerged as a potential treatment method for cancer either alone or in conjunction with chemo and radiotherapy. The major drawback during such treatment is to control the temperature and distribution of nanoparticles in vivo. In MHT, magnetic nanoparticles when subjected to an alternating current (AC) magnetic field heats the specific region of body or tissue [1]. Heat dissipation by MNPs is measured in terms of specific absorbance rate which in turn depends on magnetic moment, anisotropy energy, density, particle size and size distribution[2]. In the present talk, I will be discussing about synthesis, characterization, surface functionalization of nanocrystalline iron oxide based ferrites for MHT. Iron oxide (magntite) being a class of spinel ferrites due to its proven biocompatibility and good chemical stability is extensively studied and projected for its application in MHT. Other nanoferrites including cobalt ferrite, magnesium ferrite and mixed ferrites like Co-Zn ferrite etc have also been explored for their possible application in MHT [3]. To attain specific absorption rate at lowest particle concentrations, magnetic nanoparticles with uniform size distribution and high magnetic moment need to be synthesized. Tuning of magneto-structural properties will be achieved by choice of proper synthesis route. Once synthesized particle need to be surface functionalized with biocompatible polymers to improve colloidal stability in physiological media and cell compatibility. Finally, magnetic induction heating studies of these surface functionalized nanoferrites can be evaluated in vitro for their potential application as heating mediator in MHT for cancer. References: 1. V. M. Khot, A. B. Salunkhe, N. D. Thorat, R. S. Ningthoujam, and S. H. Pawar, Dalton Trans., 2013, 42, 1249 2. A.B. Salunkhe,, V.M. Khot and S.H. Pawar, Current Topics in Medicinal Chemistry, 2014, 14, 572-594 3. V.M. Khot, A.B. Salunkhe, J.M. Ruso, S.H. Pawar, Journal of Magnetism and Magnetic Materials 384 (2015) 335–343

Invited Presentations : The 7en minutes Report on Frontier Research
Authors : Toshifumi Imajo, Takashi Suemasu, Kaoru Toko
Affiliations : Univ. of Tsukuba

Resume : Ge has higher carrier mobility than conventional mainstream material Si, and also has a low crystallization temperature (< 500 °C), which can be synthesized onto insulators under the heatproof temperature. Therefore, Ge on insulator technology has been widely studied for lowering the fabrication cost and improving the device performance. In particular, Ge-based devices fabricated on flexible plastic substrates will open up the possibility for developing advanced wearable devices such as multi-functional display. Solid phase crystallization (SPC) is a simple method to directly form polycrystalline Ge (poly-Ge) thin films on insulating substrates at low temperatures. Recently, we found that the control of the atomic density of an amorphous Ge precursor for SPC dramatically enlarged the grain size and updated the highest record of hole mobility of semiconductor thin film on insulator [1,2]. In this study, we applied this method onto plastic substrate. As a result, the SPC-Ge on a plastic substrate exhibited higher hole mobility than that of bulk Si. This result mean that single-crystal wafers are no longer necessary for fabricating a high-mobility semiconductor thin film. Besides, the resulting hole mobility 680 cm2/Vs is the highest value to date among those of semiconductor layers directly formed on insulators at low temperatures. This achievement will give a way to realize advanced electronic and optical devices simultaneously allowing for high performance, inexpensiveness, and flexibility. [1] K. Toko et al., Sci. Rep. 7, 16981 (2017). [2] T. Imajo et al., Applied Physics Express 12, 015508 (2019).

Authors : M. Marini,1,2 S. Stassi,1 M. Allione,2 B. Torre,2 A. Giugni,2 M. Moretti,2 P. Zhang,2 C.F. Pirri,1 C. Ricciardi,1 E. Di Fabrizio, 2
Affiliations : 1 DISAT, Politecnico di Torino, Corso Duca Degli Abruzzi, 24, 10129 Torino, Italy. 2 PSE Division, KAUST, Thuwal 23955, Saudi Arabia.

Resume : Recently we obtained free-standing self-assembled biomolecules fibers, by using a physiological-compatible preparation and super-hydrophobic surfaces (SHS). A droplet of the biomolecule solution is deposited over the SHS. At room temperature, the water in solution evaporates: the droplet decreases in volume and moves from one pillar to the next. With this method the molecules in solution are pulled and linked between micro-pillars. We suspended DNA, DNA/ligands, and cell membranes that were characterized by EM, Raman Spectroscopy and Laser Doppler Vibrometer. The DNA fibers allowed us obtaining background-free TEM direct images and measuring DNA bases and backbone without the use of contrast agents, with a resolution of 1.5 Å. TEM diffraction confirmed the quantities measured. Raman spectroscopy data expanded the EM data with structural/chemical information on DNA monomers, conformation and fluctuations related to the environment. DNA bundles were also used as ultrasensitive mechanical resonators to detect and study deviations to the native form, by administering intercalants and the chemotherapy cisplatin at increasing concentration. Alteration to sizes and Young’s modulus were successfully quantified. Overall, the results show that our approach can be applied to medical-oriented developments such as the optimal chemotherapy titration and the evaluation of the effects on DNA of pollutants and contaminants such as heavy metals.

Authors : Qun Ma, Pengcheng Gao*, Fan Xia*
Affiliations : Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences (CUG), 388 lumo Road, Wuhan 430074, R. P. China.

Resume : During the decades, widespread advances have been achieved on nanochannels correspondingly, including nanochannel based DNA sequencing, single-molecular detections, smart sensors, energy transfer/storage and so on. However, researchers focus all interests on the contribution from the functional elements (FEs) at the inner wall (IW) of nanochannels, little attention has been paid on the contribution from the FEs at the outer surface (OS) of nanochannels. Herein, we achieve explicit partition of FEOS and FEIW based on accurate regional-modification of OS and IW. Furthermore, the FEIW are served for ionic gating, and the chosen FEOS (hydrophobic or charged) are served for blocking interference molecules into the nanochannels, decreasing the false signals for the ionic gating in complex environments. Furthermore, we also define a composite factor, areas of a radar map, to evaluate the FEOS performance for blocking interference molecules.

Authors : Christopher Jay T. Robidillo,1,2 Jonathan G. C. Veinot1*
Affiliations : 1Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada 2Department of Physical Sciences and Mathematics, University of the Philippines Manila, Manila 1000, Philippines *Corresponding author

Resume : This study reports the ratiometric photoluminescent detection and quantification of the toxicologically potent organophosphate ester nerve agents paraoxon (PX) and parathion (PT) using red-photoluminescent silicon-based quantum dots (SiQDs) and the green fluorescent protein mAmetrine1.2 (mAm).1 The nerve agents selectively quench SiQD photoluminescence via a dynamic quenching mechanism mediated by organophosphate nitroaromatic groups. The method reported herein afforded micromolar detection limits for PX and PT and is unaffected by inorganic and organic interferents. In addition, paper-based sensors prepared from SiQDs and mAm have been successfully employed for the detection of PX and PT at low concentrations using a color analysis smartphone application. The sensor reported herein can potentially be used in the determination of PX and PT in actual samples. Reference: 1. Robidillo, C. J. T.; Wandelt, S.; Dalangin, R..; Zhang, L.; Yu, H.; Meldrum A.; Campbell, R.E.; Veinot, J. G. C. ACS Appl. Mater. Interfaces 2019, 11, 33478-33488.

Authors : Viraj Bhingardive1,2, Guillaume Le Saux1,2 , Avishay Edri3, Angel Porgador3, and Mark Schvartzman1,2
Affiliations : Department of Materials Engineering1, Ilse Katz Institute for Nanoscale Science and Technology2, The Shraga Segal Department of Microbiology Immunology and Genetics Faculty of Health Sciences3, Ben-Gurion University of the Negev

Resume : Nanowires have been attracting great interest for numerous applications. In particular, their dimensions that are comparable to those of biomolecules, as well as their ultra-high aspect ratio and mechanical flexibility, make them very attractive for mimicking physiological environment of cells in the fundamental studies of the cell-environment intentions. To provide nanowires with the ability of chemical signaling they are often functionalized with signaling molecules. However, such a functionalization, based on either chemisorption or physisorption of biomolecules has not been site specific. Here, we took advantage of the fact that chemical-vapor-deposition grown nanowires carry on their tips metallic catalytic nanoparticles, to achieve the first of its type site selective functionalization of biomolecules. Such functionalization comes to provide a biomimetic 3D cell environment with precisely positioned biomolecules. We have grown Si nanowires from Au catalyst. We covered Au tips with biotinylated thiols, and used Neutravidin bridge to attach to the molecules of biotinylated anti-NKp30 – an activating ligands for Natural Killer cells, which are lymphocytes of the innate immune system. To study the effect of clustering at both nanometric and micron scale on cells, we investigated the effect of continues bed of nanowires verses micropatterned areas of nanowires. Further, we varied the stiffness of nanowires by changing their length and followed its effect on cell population.

Authors : Alina Vladescu1, Jürgen Schmidt2, Pinar Yilgor Huri3, Catalin Vitelaru1, Dorit Kloss2, Nesrin Hasirci4,6, Vasif Hasirci5,6, Lidia R. Constantin1, Mariana Braic1
Affiliations : 1National Institute of Research and Development for Optoelectronics - INOE 2000, 409 Atomistilor St., 077125, Magurele, Romania 2Innovent e.V., Jena, Prüssingstraße 27B, 07745 Jena, Germany 3Ankara University, Department of Biomedical Eng., Golbasi, Ankara, Turkey 4Near East University, Nicosia, Mersin 10, Turkey 5Acibadem Mehmet Ali Aydinlar Univ., Dept. Med. Eng., Istanbul, Turkey 6BIOMATEN, Middle East Technical University, Ankara 06100, Turkey

Resume : The growing implant market requires advanced Ti-based implants for long-term application, and for the newly engineered biodegradable Mg alloys with controllable dissolution rate s short-term applications. For this reason, Mg-alloys gain much more attention recently. Mg alloys are biodegradable material, but with too high corrosion rate and degradation occurs before the end of healing process. Moreover, during the corrosion, hydrogen released thereby causes pH increase of the surrounding tissue, inducing apoptosis and necrosis of tissue cells. For these reasons, it is a challenge to controlled degradation rate of Mg alloy and to provide sufficient time for the tissue to heal up to complete degradation of Mg implant. The aim of the present paper is to investigate different types of CaP coatings as possible candidate for temporary implants made of MgCa1 alloy. The coatings were prepared by magnetron sputtering and micro-arc oxidation methods. The coatings were investigated in terms of microchemical, microstructural and mechanical properties, corrosion resistance in SBF at 37°C, as well as the biocompatibility with human cell line. We acknowledge the support of the Romanian projects: no. COFUND-ERANET-RUS-PLUS-CoatDegraBac (no. 68/2018), within PNCDI III; Core Program-2020; no. 19PFE/2018 (PROINSTITUTIO).

Authors : Ph.D student Alexina Ollier1,2, Marcin Kisiel1, Urs Gysin1 and Ersnt Meyer1
Affiliations : 1 Institute of Physics, University of Basel, Switzerland 2 Swiss Nanoscience Institute, Klingelbergstrasse 82, 4056 Basel, Switzerland

Resume : Understanding nanoscale energy dissipation is nowadays among few priorities particularly in solid state systems. Breakdown of topological protection, loss of quantum information and disorder-assisted hot electrons scattering in graphene are just a few examples of systems, where the presence of energy dissipation has a great impact on the studied object. It is therefore critical to know, how and where energy leaks. Pendulum geometry Atomic Force Microscope (pAFM), oscillating like a pendulum over the surface, is perfectly suited to measure such tiny amount of dissipation, since a minimum detectable power loss is of the order of aW. Here, we report on a low temperature (T=5K) measurement of striking singlets or multiplets of dissipation peaks above graphene nanodrums surface. The stress present in the structure leads to formation of few nanometer sized graphene quantum dots (GDS) and the observed dissipation peaks are attributed to tip-induced charge state transitions in quantum-dot- like entities. In the real space the charging features are observed as Coulomb rings surrounding the GDS. The dissipation peaks and Coulomb rings strongly depend on the external magnetic field (B=0T-2T), the behavior we attributed to crossover from quantum dot carrier confinement to the confinement by magnetic field.

Authors : A.A. Romansky, V.L. Karbivskyy, V.А. Dubok, S.S. Smolyak, L.I. Karbivska
Affiliations : G.V. Kurdyumov Institute for Metal Physics of the N.A.S. of Ukraine

Resume : Previously, we developed samples of biocompatible composite based on calcium phosphates – "Syntekost" (synthetic bone). The aim of this work is to optimize the properties of bioactive materials for bone tissue recovery – ensuring their oncoprotective and anti-inflammatory properties. At various temperature conditions, a number of inorganic materials were synthesized: calcium phosphate ceramics, glasses and sitalls, as well as composites based on nanodispersed calcium hydroxyapatite. The effect of synthesis temperature conditions on the structure and properties of the obtained materials is investigated. Doping of a number of samples with bioactive elements was carried out with the aim of their functionalization. Samples of conductive bioactive nanocomposites based on nanodispersed apatite and nanodispersed graphite were obtained, and their properties were studied by electrophysical methods. An analysis of the XPS data of the obtained samples showed that, depending on the conditions of glass synthesis – melt cooling rate, temperature regime, and glass doping, the binding energy of the constituent elements changes. The temperature conditions of the process for producing such materials were optimized. The relative fraction of bioactive calcium phosphates and their crystallinity correlate with synthesis conditions, in particular – temperature regime. The XPS data for the fused glass sample indicate the presence of CaSiO_3 and Mg_2SiO_4, which are associated with bio-sitalls.

Authors : Siaka Fadera, I-Chi Lee
Affiliations : Chang Gung University

Resume : The existence of cancer stem cells (CSCs) has been demonstrated in several solid tumors including brain cancer. These CSCs represent a sub-population of cells within tumor bulk that exhibit stem-cell-like characteristics and they are believed to play a significant role in tumor formation and recurrence, metastasis and they are resistant to conventional chemotherapeutic drugs. They are enriched in stem cells markers and markers associated with higher drug resistance and self-renewal. Chemotherapy is a well-accepted treatment modality for different cancer types including glioblastoma, however, its efficacy on glioblastoma still remains far from the best in most cases. The chemotherapeutic drugs are mostly effective on normal cancer cells leaving glioblastoma (GBM) stem-like cells insignificantly affected after treatment. Therefore, the establishment of better treatment strategy is needed in order to combat glioblastoma stem-like cells drug resistance. One such strategy is the induction of differentiation on GBM CSCs to normal cancer cells that will circumvent their self-renewal ability and improve their sensitivity to anti-cancer drugs. Previous literature has used low-intensity ultrasound (LIPUS) to inhibit the proliferation and induce apoptosis in cancer cells. Additionally, we have reported the induction effect of LIPUS on liver CSCs. In the present study, LIPUS was deigned to induce differentiation and concomitantly enhance the sensitivity and an uptake of drug by CSCs isolated from GBM cell line. We first designed and developed three dimensional tumor microenvironment using hyaluronic acid (HA) based polyelectrolyte multilayer (PEM) film system in order facilitate the isolation and enrichment of GBM CSCs. The next step is the use of LIPUS stimulation of GBM CSCs in combination with anti-cancer loaded gold nanoparticles. Colony formation, migration and invasion, putative CSCs marker expression, drug resistance, stem cell related genes expression and immunostaining were all investigated and compared with control group. The results revealed that high colony formation was observed on PEM system after 3 days of culture which continued through 7 days after culture. Higher expression of CD133, higher migration and invasion ability, up-regulation of proteins and genes related to CSCs and an increased drug resistance capacity were observed in cells isolated on PEM film system. From our previous study, LIPUS stimulation may result to down- regulation of CSCs colonies, down-regulation of CSCs related proteins and genes, down-regulation of migration and invasion ability, and an enhanced drug sensitivity of CSCs. Our proposed study design may provide alternative therapeutic strategy on the differentiation and effective combination therapy of glioblastoma CSCs.

Authors : Alina Sharova, Mario Caironi
Affiliations : Dipartimento di Fisica, Politecnico di Milano, P.zza L. da Vinci 32, 20133 Milan, Italy; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia, Via Pascoli 70/3, 20133 Milan, Italy

Resume : Going beyond the traditional concept of electronic devices, we convey the idea of making electronics edible. This unconventional approach exploits the electronic properties of natural and food-based materials for developing ingestible functional devices. Critical biomedical, pharmaceutical, and food industry applications are targeted by the proposed field. In this framework, we explore the potential of cost-effective and edible substance, honey, to be used as electrolytic gate viscous dielectric. Honey-gated organic field effect transistors (OFETs) based on both n & p type semiconductors are fabricated. A distinctive feature of these transistors is their long-term stability, reproducibility and low voltage < 1V operation in air. Devices exhibit forward-looking electronic performances, notably, electron and hole mobility–capacitance product of 3.5 × 10–3 μF/Vs and 23 × 10–3 μF/Vs, respectively, surpassing ones of the previously reported water-gated OFETs. Furthermore, the observed devices responsivity to humidity provides promising opportunities for sensing applications. We then demonstrate, for the first time, the implementation of honey-based integrated circuits: inverting logic gate and ring oscillator.

Authors : Rotar, D.V. (1), Kobasa, I.M. (2), Kornyakov A. O. (3), Vorobets, M.M. (4)
Affiliations : (1) PhD, Departments of Microbiology and Virology Bukovina State Medical University, 2, Theater Square, Chernivtsi, 58002, Ukraine E-mail: (2)Yu. Fedkovych Chernivtsi National University., Ukraine 2 Kotsiubynskiy str., Chernivtsi 58012 E-mail: (3) Bukovyna State Medical University, Ukraine 3rd year student of medical faculty Bukovina State Medical University, 2, Theater Square, Chernivtsi, 58002, Ukraine Email: (4)Yu. Fedkovych Chernivtsi National University. , Ukraine 2 Kotsiubynskiy str., Chernivtsi 58012 E-mail:

Resume : Antibiotic resistance is one of the most pressing problems of modern medical practice. This situation should be addressed through the rational and correct prescribing antimicrobial agents in clinics. Changes in Ukraine's health care system have the need for reconstruction and restoration of medical facilities. The use of ordinary repair materials for this purpose can not be used. Studies have shown the dependence of adhesive and colonization properties of clinical antibiotic-resistant strains of microorganisms from the effects of materials which based on TiO 2 Degussa P25 with photocatalytic activity under UV with a wavelength of 365 nm with a different exposure time. The experiment was carried out by the method of diffusion, a microorganism lawn culture was sown on a cup with a nutrient medium, a part of the cup was covered with a light-proof material, and placed in a light chamber (the duration of irradiation of the lawn was different) . After 24 hours cultivation of lawn cultures of antibiotic-resistant strains of microorganisms were recorded. Established that material with nano-TiO 2 under UV irradiation him with a wavelength of 365 nm resulted in stunted growth and development of culture lawn clinical strain of microorganism. Thus, from a culture with a microbial load of 10^5 cells / ml on the control samples developed a dense lawn, indicating the regeneration of the culture, its adhesion to the surface and colonization of the surface. Nano-TiO 2 at UV with a wavelength of 365 nm caused a delay in the development of all cultures of clinical strains of microorganisms. Thus, from the working suspension with a microbial load of 10^5 cells / ml grew single colonies in an amount of from 2 to 4 CFU. Materials based on Degussa P25 TiO 2 are promising for the manufacture of surfaces of medical equipment where there is a threat of colonization by antibiotic resistant strains of microorganisms.

Authors : Francesca Guagnini, Peter B. Crowley
Affiliations : School of Chemistry, National University of Ireland, Galway, Ireland

Resume : Thanks to their versatility, proteins are ideal building blocks for functional materials. Controlled protein assembly is necessary for bottom-up fabrication of biomaterials.[1] Many strategies can be employed to achieve protein assembly, like computational design of protein interfaces, supercharged proteins or tethered linkers.[1] The use of macrocyclic receptors is a viable alternative. Macrocyclic receptors, like cucurbiturils or calixarenes, recognize patches on protein surfaces and induce assembly / crystallization by bridging two or more proteins. This transferable approach has been demonstrated in different proteins and does not require expensive protein functionalization or the use of designed proteins.[2-3] Metal coordination is another directional interaction employed to facilitate protein assembly.[4] We postulated that macrocycle recognition coupled with metal coordination will increase the control over assembly design. To demonstrate this idea, we tested two different protein model systems: a β-propeller lectin and a highly cationic small protein. Our research paves the way for new biohybrid materials. [1] Luo, Q.; Hou, C.; Bai, Y.; Wang, R.; Liu, J., Chem. Rev., 2016, 116, 13571–13632. [2] Rennie, M. L.; Fox, G. C.; Pérez, J.; Crowley, P. B., IUCRJ, 2019, 6, 238-247. [3] Guagnini, F.; Antonik, P. M.; Rennie, M. L.; O’Byrne, P.; Khan, A. R.; Pinalli, R.; Dalcanale, E.; Crowley, P. B., Angew. Chemie Int. Ed., 2018, 57, 7126–7130. [4] Bailey, J. B.; Zhang, L.; Chiong, J. A.; Ahn, S.; Tezcan, F. A., J. Am. Chem. Soc., 2017, 139, 8160–8166.

GENERAL POSTER SESSION : Chairs: Insung S. Choi (Korea), Peter Scharff (Germany), Emmanuel Stratakis (Greece), Thomas J. Webster (USA), Eugenia Buzaneva and Oleksandr Ivanyuta (Ukraine)
Authors : R.M. Dodo1, M. Abdulwahab1,2, U. Shehu1, F. Asuke1, A. Hamisu1
Affiliations : 1Department of Metallurgical and Materials Engineering, Ahmadu Bello University, Zaria, Nigeria 2Department of Chemical, Metallurgical and Materials Engineering, Tshwane University of Technology, Pretoria, South Africa

Resume : The present study explored wear and biocompatibility characteristics of polypropylene (PP)/ periwinkle shell particulates (PWS) composites for dental implant application. Wet-sliding the stainless steel ball against specimen disc revealed the abrasive wear behaviour of the composites under load and linear speed of 5N and 10 cm/s respectively. In the wear tests, the specimens were wetted with simulated human saliva with and without sodium fluoride (NaF) additive. On the other hand, a mouse specimen was used to carry out a biocompatibility test. The composite demonstrated superb wear resistance at 40 and 50wt% reinforcement loading. Further, results showed that wear rate decreased considerably with increasing PWS content. Higher wear rate was noted on composites under condition of simulated human saliva with NaF addition. The biocompatibility test reveals that developed composite is harmless to the living tissues. Thus, PP/PWS composites could be recommended to be used as dental restorative material.

Authors : Toshifumi Imajo, Takashi Suemasu, Kaoru Toko
Affiliations : Univ. of Tukuba

Resume : To realize high-performance thin-film transistors, solid-phase crystallization (SPC) of Ge films on insulators has been widely investigated, which is a simple method to directly form polycrystalline thin films on insulators. Recently, we succeeded in increasing the grain size of the Ge thin film on the glass substrate by controlling the density of amorphous Ge, which is the precursor of SPC, and have continued to update the highest record of mobility of the low-temperature synthetic film [1,2]. In this study, we develop this SPC-method onto plastic substrate. In the experiment, GeO2 underlayer was sputtered on both SiO2 glass and plastic substrates. After that, amorphous Ge precursors were prepared by molecular beam while heating the samples at 150 °C. Finally, the samples were then loaded into a conventional tube furnace in a N2 atmosphere and annealed (375 °C, 150 h) to induce SPC. As a result, SPC-Ge formed on a plastic has almost the same crystal grain size as that on a glass. By examining the electrical properties of substrate dependence of Ge layer, we found that there is almost the same tendency of electrical properties between the samples on plastic and glass substrates. With the above, we performed post annealing (500 °C, 5 h) in order to reduce the grain boundary barrier and the impurity scattering, and achieving hole mobility of 680 cm2/Vs for Ge thin film on plastic substrate. It is the highest value to date among those of semiconductor layers directly formed on insulator and is a result for exploiting excellent flexible devices. [1] K. Toko et al., Sci. Rep. 7, 16981 (2017). [2] T. Imajo et al., Applied Physics Express 12, 015508 (2019).

Authors : Professor Dr. Tatsuo Arai
Affiliations : Department of Systems Innovation, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan ,

Resume : Research in a new field, Hyper Bio Assembler for 3D Cellular Innovation (Bio Assembler) started in July 2011 as a five year project supported by Grants-in-Aid for Scientific Research on Innovative Areas from Japanese Ministry of Education (MEXT). The aim of project is to build 3D cellular systems capable of functioning in vitro and to propose an entirely new interdisciplinary area never previously explored. The Bio Assembler will elucidate the principles of ultrahigh speed measurement as well as the manipulation techniques and tissue function expression. The project consists of three research groups; first, “Measurement and control of cell characteristics” which focuses on measuring physical properties of cells taken from living organisms at high speed and separating target cells useful in constructing cellular systems; second, “3D cellular system assembly” which aims at shaping and assembling 3D cellular systems with complex morphologies and vascular networks inside; third, “Analysis and evaluation of 3D cellular systems” which provides analysis and evaluation of growth, differentiation-inducing, morphogenesis controls and transplantation responses of created 3D cellular systems, and conducts functional elucidation as well as comparison with and verification of in vivo with looking for ways to bring our outcomes into the tissue life science such as regenerative medicine, cell assay, and so on. Our final goal is to establish three major academic achievements; “Cell Sort Engineering”, “3D Cellular System Design Logic” and “Cell Sociology” as well as providing innovative measurement and control technologies based on micro-nano robotics.

Authors : Pengxiang Si, Fan Jiang, Qingsha S. Cheng*, Geoffrey Rivers, and Boxin Zhao*
Affiliations : P. Si, Dr. G. Rivers, Prof. B. Zhao Department of Chemical Engineering Waterloo Institute for Nanotechnology University of Waterloo 200 University Avenue West, N2L 3G1, Canada F. Jiang, Prof. Q. S. Cheng Department of Electrical and Electronic Engineering Southern University of Science and Technology 1088 Xueyuan Ave, 518055, China

Resume : Antennas are an essential component of next generation wearable electronics for wireless communication. It is highly desirable to develop a robust self-healable antenna to maintain the integrity and function of the device because the wearable devices are becoming ever-thinner and smaller, making them subject to serious wear and damage during the body bending or stretching. However, it has been a significant material challenge as the integral antenna can not be exposed to external stimulus such as heat, light and chemical agents without damaging the devices. Herein, we design a latex-polyelectrolyte coacervation (LPC) system compounded with conductive filler to form a printable conductive paste which exhibits robust toughness, stiffness, extensibility, high electrically conductivity, one-step printability and self-healing capability simultaneously. The formation of polymer network via electrostatic interaction and hydrophobic interaction within antenna provide a self-healing mechanism via the absorption of moisture in the ambient air. The LPC system is promising for broad applications in self-healable stretchable electronics.

Authors : Oleksandr Ivanyuta1, Eugenia Buzaneva2, Uwe Ritter3, Peter Scharff3
Affiliations : 1 Faculty of Radiophysics Electronics and Computer Systems, Taras Shevchenko National University of Kyiv, Ukraine E-mail: 2 The Scientific and Training Center on Physical and Chemical Material Science, National Taras Shevchenko National University of Kyiv and NAS of Ukraine 3 Institute of Chemistry & Biotechnology, T U Ilmenau, Germany

Resume : Biofunctionalization of carbon nanotubes, which are well-known for their unique complex of properties, is in the centre of research attention for numerous biomedical applications, also as for fundamental study of nanotubes as templates. Covalent or non-covalent immobilization of biomolecules on carbon nanotubes opens the doors for novel biocomposite manufacturing and biosensing. Carbon nanotube functionalization by DNA, RNA, oligonucleotides or single nucleotides is of special interest as possible approach for nucleic acid sensing, gene delivery, gene therapy, clinical diagnosis and pathogen monitoring. It is also used in nanotechology as technique for suspensioning, characterization and separation of nanotubes. In our investigations the non-covalent immobilization in aqueous suspensions of AMP and DNA on multi-walled carbon nanotubes developed. Previous oxidation of multi-walled carbon nanotubes by ozonolysis were used if it was necessary. The characterization of functionalized nanotubes was carried out by Scanning Electron Microscopy, Transmission Electron Microscopy, Optical Microscopy, Raman, UV-visible-NIR, IR- and Photoluminescence Spectroscopies. Various models of interface formation between biomolecules and nanotube surface are discussed. Optical characteristics of novel biofunctionalized nanotubes also gave us necessary information for their future application as biosensors or activating agents.

Authors : Monica Marini 1, Bruno Torre 2, Marco Allione 2, Maria Caterina Morello 2, Manola Moretti 2, Andrea Giugni 2, Enzo di Fabrizio 2
Affiliations : 1 DISAT, Politecnico di Torino, Corso Duca Degli Abruzzi, 24, 10129 Torino, Italy. 2 SMILEs Lab, PSE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia

Resume : Oriented and self-organized DNA filaments were obtained by micro-fabricated super-hydrophobic surfaces (SHS). The bio-macromolecule, suspended over the SHS, can be characterized by using several techniques, such as electron microscopy and optical spectroscopy. DNA direct imaging by high resolution TEM (HRTEM) allowed solving the base pairs with a resolution of 1.5 Å [1,2] and the metrological details can be effectively corroborated by Raman spectroscopy data [3,4]. Raman spectra in the range 600-1800 cm-1 were acquired on suspended DNA filaments and on the droplet residual. The spectra obtained at the same working conditions on DNA samples and buffer deposited over a CaF2 window were used as negative control. The study of the spectra revealed the absence of physiologically compatible buffers on suspended filaments while their contribution is strong in the DNA spectra acquired on CaF2 windows and on the droplet residual. This suggests that the optimized SHS platform separates small molecules from the suspended DNA and the non-interacted material is concentrated in the droplet residual. The SHS-DNA platform revealed a strong potential for the study of polarized Raman spectra as the DNA filaments are autonomously oriented with different angles over the device and for the analysis of the presence and influence of molecules affecting the DNA double helix such as chemotherapeutic compound (Cisplatin), heavy metals and methylations.

Authors : Alexina Ollier1,2, Marcin Kisiel1, Urs Gysin1 and Ernst Meyer1
Affiliations : 1 Institute of Physics, University of Basel, Switzerland 2 Swiss Nanoscience Institute, Klingelbergstrasse 82, 4056 Basel, Switzerland

Resume : Understanding nanoscale energy dissipation is nowadays among few priorities particularly in solid state systems. Breakdown of topological protection, loss of quantum information and disorder–assisted hot electrons scattering in graphene are just few examples of systems, where the presence of energy dissipation has a great impact on the studied object. It is therefore critical to know, how and where energy leaks. High sensitivity pendulum geometry Atomic Force Microscope (AFM), oscillating like a pendulum over the surface, is perfectly suited to measure tiny amount of dissipation. The tip position on the sample is controlled with atomic accuracy owing to a tunneling current line and the enhanced sensitivity allows to distinguish between electronic, phononic or van der Waals types of dissipation. Measurements can be performed in a wide range of temperatures from 5K to room temperature and in magnetic fields spanning from B=0T to B=7T. The design of the sample holder allows to perform dissipation measurements while passing electric current in the plane of the sample surface. In this work we performed energy dissipation measurements on a suspended graphene sheet at room temperature under UHV. The graphene is deposited on a hole patterned substrate in order to have suspended circular (with a diameter of 6.5 µm) graphene sheet. The experiments allows to investigate the phononic and electronic energy dissipation of the suspended graphene.

Authors : Happy Agarwal; VenkatKumar Shanmugam*
Affiliations : School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore – 632014, TN, India *Corresponding Author Mail id:;

Resume : In the present study, we have biosynthesized ZnO NPs and investigated the mechanism of anti-inflammatory action of the nanoparticles in both in vitro and in vivo models. In the in vitro model system, RAW 264.7 murine macrophages were pre-incubated with ZnO NPs and the effect of NPs on the lipopolysaccharide (LPS)-induced activation of nuclear factor-kappa B (NF- κB) pathway was evaluated. Results show that LPS mediated increase in expression NF- κB pathway was downregulated by an increase in expression of inhibitor of kappa B alpha (IκBα) in NP treated cells in a dose-dependent manner. The anti-inflammatory potential of ZnO NPs was also evaluated in Carrageenan induced mice paw edema model. Administration of ZnO NPs to mice, prevented Carrageenan induced mice paw edema and also suppressed the expression of pro-inflammatory mediators like interleukin-1 beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), and cyclooxygenase-2 (COX-2). Histological analysis of mice paw tissue section clearly indicated a reduction in leukocyte and neutrophil extravasation to the tissue space in mice paw that was pre-treated with ZnO NPs. Present findings give us an insight into the ZnO NPs mediated anti-inflammatory action.

Authors : Soumya Menon, Dr.Venkatkumar S.
Affiliations : School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore 632014, India

Resume : Cervical cancer is the most common malignancy suffered by women worldwide in the female reproductive system. The chemotherapeutic treatment using anticancer drugs are developed recently, but they lack delivery to the targeted tumour tissues. The commonly used anticancer drug paclitaxel is highly efficient against various cancer types, but due to its low solubility the enhancement of its property decreases. Recent advances includes nanotechnology based combinatorial drug delivery systems that offer enhanced pharmacokinetic property due the EPR (enhanced permeable retention), the larger surface area to volume ratio in nanoparticles, it also helps in adsorbing large amount of drug and act as a drug carrier. The combination of nanosystems aids in active or passive drug delivery through the cell membranes via endocytosis. In particular selenium nanoparticles is a well established chemotherapeutic agent due to low toxicity to the normal tissues, when compared to other nanosystems. The selenium nanoparticles act as both prooxidant in the presence of cancer tissues by producing ROS which eventually causes oxidative stress, mitochondrial damage, alter the genetic arrangements of the cancerous tissues, they are also major component of many antioxidant enzymes that also function at protecting from tumour like GSH (glutathione), selenomethioninecystien (recent established amino acid), theoredoxin (Trx) and so on. They act as antioxidant agent for normal tissues by scavenging ROS, regulating the DNA repair proteins, and protecting the damages caused by oxidative stress. In this paper more emphasis is done on the green synthesis of selenium nanoparticles to avoid the utilization of external harmful chemicals. The precursor used for the reduction of the sodium selenite which itself it a harmful chemical which was traditionally used as anticancerous agent, are Mucuna pruriens seed extract which is also a traditional medicine against various cancer types. The extract is used as a bioreductive catalyst which also helps in stabilization of the nanoparticle development. The biosynthesized SeNPs are optimized using BB design for evaluating the optimum particle size and process variable. The optimized SeNPs are then coated with chitosan which acts a biodegradable linker against anticancer drug paclitaxel. The combinatorial nanosystem is evaluated for its anticancerious activity against HeLa cancer cells. This innovative strategy can help in overcoming the drawbacks faced by chemotherapeutic drugs with low solubility, delivery to the targeted sites, highly biodegradable and low toxicity.

Authors : A.A. Romansky, V.L. Karbivskyy, V.А. Dubok, S.S. Smolyak, L.I. Karbivska
Affiliations : G.V. Kurdyumov Institute for Metal Physics of the N.A.S. of Ukraine

Resume : Previously, we developed samples of biocompatible composite based on calcium phosphates – "Syntekost" (synthetic bone). The aim of this work is to optimize the properties of bioactive materials for bone tissue recovery – ensuring their oncoprotective and anti-inflammatory properties. At various temperature conditions, a number of inorganic materials were synthesized: calcium phosphate ceramics, glasses and sitalls, as well as composites based on nanodispersed calcium hydroxyapatite. The effect of synthesis temperature conditions on the structure and properties of the obtained materials is investigated. Doping of a number of samples with bioactive elements was carried out with the aim of their functionalization. Samples of conductive bioactive nanocomposites based on nanodispersed apatite and nanodispersed graphite were obtained, and their properties were studied by electrophysical methods. An analysis of the XPS data of the obtained samples showed that, depending on the conditions of glass synthesis – melt cooling rate, temperature regime, and glass doping, the binding energy of the constituent elements changes. The temperature conditions of the process for producing such materials were optimized. The relative fraction of bioactive calcium phosphates and their crystallinity correlate with synthesis conditions, in particular – temperature regime. The XPS data for the fused glass sample indicate the presence of CaSiO_3 and Mg_2SiO_4, which are associated with bio-sitalls.

Authors : Viraj Bhingardive1,2, Guillaume Le Saux1,2 , Avishay Edri3, Angel Porgador3, and Mark Schvartzman1,2
Affiliations : Department of Materials Engineering1, Ilse Katz Institute for Nanoscale Science and Technology2, The Shraga Segal Department of Microbiology Immunology and Genetics Faculty of Health Sciences3, Ben-Gurion University of the Negev

Resume : Nanowires have been attracting great interest for numerous applications. In particular, their dimensions that are comparable to those of biomolecules, as well as their ultra-high aspect ratio and mechanical flexibility, make them very attractive for mimicking physiological environment of cells in the fundamental studies of the cell-environment intentions. To provide nanowires with the ability of chemical signaling they are often functionalized with signaling molecules. However, such a functionalization, based on either chemisorption or physisorption of biomolecules has not been site specific. Here, we took advantage of the fact that chemical-vapor-deposition grown nanowires carry on their tips metallic catalytic nanoparticles, to achieve the first of its type site selective functionalization of biomolecules. Such functionalization comes to provide a biomimetic 3D cell environment with precisely positioned biomolecules. We have grown Si nanowires from Au catalyst. We covered Au tips with biotinylated thiols, and used Neutravidin bridge to attach to the molecules of biotinylated anti-NKp30 – an activating ligands for Natural Killer cells, which are lymphocytes of the innate immune system. To study the effect of clustering at both nanometric and micron scale on cells, we investigated the effect of continues bed of nanowires verses micropatterned areas of nanowires. Further, we varied the stiffness of nanowires by changing their length and followed its effect on cell population.

Authors : Geun-Tae Yun, Woo-Bin Jung, and Hee-Tae Jung*
Affiliations : Korea Advanced Institute of Science and Technology (KAIST)

Resume : Both high static contact repellency and resistance to liquid drop impact are quite necessary to achieving a high-performance omniphobic surface. The cuticles of springtails, a small insect in nature, have both of these features, which result from their hierarchical structure composed of primary nanostructures on secondary microgrooves. Despite intensive efforts, none of the previous reports that were inspired by the springtail were able to achieve both high static repellency and pressure resistance simultaneously due to a general trade-off between these features. We demonstrate for the first time a springtail-inspired superomniphobic surface showing both features by fabrication of a hierarchical structure consisting of serif-T–shaped nanostructures on microscale wrinkles. Our biomimetic engineering yielded a surface having high repellency to diverse liquids, from water to ethanol, with an apparent contact angle above 150°. The surface was also able to show extreme pressure resistance from the impacts of diverse liquid drops, which is the best pressure resistance on omniphobic surface ever reported.

Authors : Priya Mullick 1 , Gopal Das 2* and Aiyagari Ramesh 1*
Affiliations : 1 Department of Biosciences and Bioengineering, 2 Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Tel: 91-361-2582313; Fax: 91-361-2582349 (Gopal Das) Tel: 91-361-2582205; Fax: 91-361-2582249 (Aiyagari Ramesh) E-mail: (Gopal Das); (Aiyagari Ramesh)

Resume : Herein, we report a biogenic route of hydroxyapatite nanoparticle (HANP) synthesis either in presence of the whole cell (WC) of the lactic acid bacteria (LAB) Lactobacillus rhamnosus GG or a malonic acid amphiphile (MA). HANPs were generated by in situ precipitation and the characteristic peaks at 3568, 1461, and 1041 cm-1 observed in FTIR analysis and the prominent peaks at around 2θ = 26° and 2θ = 33° in PXRD analysis revealed that the LAB cells and MA could generate hydroxyapatite phase. FESEM revealed that WC-HANPs were spindle-shaped and 70-110 nm in size while MA-HANPs were spherical and 22-27 nm in size. HRTEM indicated that the lattice distance for WC-HANPs and MA-HANPs was 0.28 nm and 0.29 nm, respectively, while SAED confirmed the crystallinity of HANPs. TGA analysis indicated degradation of WC-HANPs and MA-HANPs from 20°C to 800°C, with 83% and 87% residual mass, respectively. BET analysis indicated that WC-HANPs and MA-HANPs had a surface area of 170.47 m²/g and 90 m²/g, respectively, and an average pore size of 4.9 nm and 4.04 nm, respectively. Both the HANPs were non-toxic to cultured osteosarcoma MG-63 cells. Fluorescence microscopic analysis revealed that the MG-63 cells grown in presence of HANPs appeared elongated, suggesting that HANPs likely support bone cell differentiation. The biogenic route of HANP synthesis outlined herein is an interesting prospect to generate potentially biocompatible nanomaterials for tissue engineering applications.

Authors : Alina Sharova, Mario Caironi
Affiliations : Dipartimento di Fisica, Politecnico di Milano, P.zza L. da Vinci 32, 20133 Milan, Italy; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia, Via Pascoli 70/3, 20133 Milan, Italy

Resume : The use of natural and bioinspired materials is an emerging key approach towards development of new-generation safe technology. Going beyond the traditional concept of electronic devices, we convey the idea of making electronics edible. This unconventional approach exploits the electronic properties of natural and food-based materials for developing ingestible functional devices. Critical biomedical, pharmaceutical, and food industry applications are targeted by the proposed field. In this framework, we explore the potential of cost-effective and edible substance, honey, to be used as electrolytic gate viscous dielectric. Honey-gated organic field effect transistors (OFETs) based on both n & p type semiconductors are fabricated. A distinctive feature of these transistors is their long-term stability, reproducibility and low voltage < 1V operation in air. Devices exhibit forward-looking electronic performances, notably, electron and hole mobility–capacitance product of 3.5 × 10–3 μF/Vs and 23 × 10–3 μF/Vs, respectively, surpassing ones of the previously reported water-gated OFETs. Furthermore, the observed devices responsivity to humidity provides promising opportunities for sensing applications. We then demonstrate, for the first time, the implementation of honey-based integrated circuits: inverting logic gate and ring oscillator. Lastly, honey-gated OFETs are fabricated on edible flexible tattoo-paper substrate that acts as a versatile platform for organic edible electronics [1]. [1] Giorgio E. Bonacchini, Caterina Bossio, Francesco Greco, Virgilio Mattoli, Yun‐Hi Kim, Guglielmo Lanzani, and Mario Caironi, Tattoo‐Paper Transfer as a Versatile Platform for All‐Printed Organic Edible Electronics, Advanced Materials 30 (14): 1706091, 2018

Authors : Sangita Kundu, and Nilmoni Sarkar
Affiliations : Senior Research Fellow, Department of Chemistry, Indian Institute of Technology Kharagpur-721302 West Bengal India; Professor, Department of Chemistry, Indian Institute of Technology Kharagpur-721302 West Bengal India

Resume : In vivo thermometry is an emerging area of research to understand the temperature regulation within and between the organs and to describe the intricate cellular metabolic processes. Highly green luminescent gold nanoclusters (Au NCs) have been successfully implemented as potential sensors for temperature measurement in vivo because of their small size, biocompatibility, quick equilibration with temperature and most importantly their response reflect the thermal environment in the physiological temperature range. This thermoresponsive fluorescence lifetime based nanoprobe based on the supramolecular host-guest recognizition employing 6-aza-2-thiothymine and L-arginine (L-Arg) shows selective cytoplasm staining and robust response to temperature. Subsequently, fluorescence lifetime imaging microscopy (FLIM) has been employed to elucidate intracellular nanothermometry inside living cells. In depth understanding of protein-Au NCs interaction offer a promising avenue to rationally design target-specific fluorescent biolabeling agent. Because of the small surface area of the Au NCs, these probes open up the new possibility of combining therapeutic moieties having the specific cytoplasm targeting capability and simultaneously monitor the temperature assisted drug- delivery/release.

Authors : Hyoung-Joon Jin
Affiliations : Inha University

Resume : Recently, nanocellulose has gained much attention as a potential reinforcing material in the field of composites owing to its excellent mechanical, biodegradable, renewable, and biocompatible properties. Cellulose nanocrystals (CNCs), which combine natural abundance and excellent mechanical properties, are a promising candidate for use as polymer reinforcement agents. CNCs are employed in a variety of fields to enhance the mechanical properties of products such as cosmetics, pharmaceuticals, paper coatings, and additives in paper and paperboard. The high density of hydroxyl groups provides the hydrophilic properties of these materials, making them also suitable for the production of hydrogels. However, because of their hydrophilic nature, CNCs are mainly used to control the hydrophilic properties and improve the mechanical properties of hydrophobic polymers, especially synthetic polymers. On the other hand, in the case of hydrophilic polymers, an additional physicochemical cross-linking process is required because CNCs only affect the mechanical properties of composite materials. In this work, dual functionalized-CNCs as a nanofiller was used to develope a gelatin-based nanocomposite film with enhanced mechanical properties and hydrolytic stability . Through the sodium periodate oxidation reaction, C2-C3 cleavage of cellulose molecules was generated and the aldehyde groups were introduced on these carbon atoms. Using dialdehyde CNCs, interfacial covalent bonding was formed between the aldehyde group of the CNCs and the amine group of the gelatin molecule through a nucleophilic addition reaction. To investigate the effect of the dialdehyde CNC content on the properties of the biocomposite, the mechanical and physicochemical properties and the hydrolytic stability of the fabricated biocomposite films were studied using various analysis techniques. The bi-functional CNCs, which can have both cross-linking and reinforcing effects, would expand the fabrication and exploration of high-performance biopolymer nanocomposites. The gelatin-based nanocomposite film developed by this strategy has greatly improved mechanical properties and hydrolytic stability, which suggests that gelatin can be used not only as a hydrophilic polymer but also as a universal polymer.

Authors : Doohun Kim, Kyeong-Hee Lee, Bongju Kim, Minwoo Kim
Affiliations : Korea Electro-technology Research Institute (KERI), Changwon 51543, Republic of Korea; Dental Life Science Research Institute & Clinical Translational Research Center for Dental Science, Seoul National University Dental Hospital, Seoul 03080, Republic of Korea; Dentis Ltd., Daegu 41065, Republic of Korea

Resume : To increase bone regeneration around a titanium implant, nanopattern surfaces with 10 nm nanopores in 120 nm dimples were produced by electrochemical nanopattern formation (ENF). The ENF surfaces were obtained by removing the TiO2 nanotube layers prepared by an anodization process. To determine the effect of the ENF surface in vitro, cell proliferation assays, alkaline phosphatase (ALP) activity assays, Alizarin red staining, western blotting, and immunocytochemistry were performed. Atomic force microscopy and scanning electron microscopy analysis showed that the ENF surface had an ultrafine surface roughness with highly aligned nanoporous morphology. In vitro, human mesenchymal stem cells exhibited increased cell proliferation and enhanced expression of osteogenic molecules such as ALP, osteopontin, and osteocalcin on ENF surfaces compared to the TiO2 nanotube surfaces. Therefore, ENF is a promising technique for coating osteointegrative implant materials.

Authors : Siyu Xiong, Prof Andrew Davenport, Prof Kwang-Leong Choy
Affiliations : Siyu Xiong, Prof Kwang-Leong Choy: Institute for Material Discovery, University College London, London, WC1E 7JE, United Kingdom Prof Andrew Davenport: Centre for Nephrology, Royal Free Hospital, London NW3 2PF, United Kingdom

Resume : Indoxyl sulfate (IS) has been reported to not only accelerate the progression of chronic kidney disease (CKD), but also increase the risk of cardiovascular disease by increasing the oxidative stress of CKD patients. Current haemodialysis facilities are immobilized due to huge dialysis machine and dialysate bag. Some wearable artificial kidney (WAK) devices are still based on diffusion principles, which could not fundamentally solve the problem of portability. We report on a novel IS absorbent material based on biocompatible polycaprolactone-chitosan (PCL-CS) nanocomposite fibrous structure. This composite absorbent material has a high internal surface to volume ratio so as to greatly increase the interaction between the IS and chitosan, which exhibits character of high binding affinity to IS molecules. The PCL-CS absorbent material was fabricated by 2D-controlled close-range electrospinning. The surface morphology and chemical composition were characterized by a combination of scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The IS molecule absorption of the PCL-CS absorbent was quantitatively evaluated. This novel nanocomposite absorbent may provide new sights for the removal of metabolic toxins in a portable way.

Authors : Kaiwei Tang(1)*, Guomin Wang(1), Paul K. Chu(1).
Affiliations : (1) City University of Hong Kong, China

Resume : Au decorated aligned ZnO nanorods are synthesized on the Ti substrate by the silane coupling agent-assisted seed layer formation and hydrothermal process with the following magnetron sputtering of gold nanoparticles. The Au@ZnO systems inactivate about 80% of Escherichia coli in the first 1h culture in the dark through an extracellular electron transfer mechanism instead of ROS production and zinc ion releasing. Electrons on the respiratory chains of the bacterial membrane transfer to the surface of the materials, making bacteria lose electron until death. At the same time, the transferred extracellular electrons convert into the bacterial current by the electrochemical workstation, primarily demonstrating the property of the materials to function as a sensor of bacteria counting and status observation. This work sheds light on the detailed understanding of antibacterial behaviors of ZnO-based materials especially at the early stage of bacteria-material contact and provides insights for the designing of devices with both antibacterial and detection applications.

Authors : Tae Ann Kim*, Jun Beom Pyo, Sang-Soo-Lee, and Min Park
Affiliations : Photo-electronic Hybrids Research Center, Korea Institute of Science and Technology, Republic of Korea

Resume : Developing materials that can absorb large amounts of strain without fracture or significant degradation in their electronic properties is important to realize stretchable electronics. Here, stretchable conductors made from reduced graphene oxide/polydimethylsiloxane (r-GO/PDMS) composite are fabricated to achieve stable resistance changes under uniaxial stretching. By controlling the weight fraction of GO in water, highly aligned and porous GO structure is obtained over 0.5 wt% of GO solution. After thermal and chemical reduction of GO to r-GO film, PDMS is infiltrated into pores of the film to make free-standing and elastic composites. Electrical percolation of r-GO in the composite is achieved at the r-GO concentration over 0.3 wt% along with improvement of electrical conductivity. However, highly aligned r-GO/PDMS composite only exhibits notable suppression of resistance change under the large deformation of 100% strain. The resistance change of 0.3 wt% r-GO/PDMS composites at 100% strain is over 7 times higher than that of 0.7 wt% r-GO/PDMS composites, exhibiting the dramatic effect of r-GO alignments. Even after repeated stretching/releasing cycles, the variation in resistance from 0 to 70% of strain is stable. The stretchable conductor based on the aligned r-GO/PDMS composite was successfully applied as a resistive strain sensor.

Authors : N. Tsierkezos, U. Ritter, P. Scharff (2), O.Ivanyuta, E. Buzaneva (1)
Affiliations : 1National Taras Shevshenko University of Kyiv, 64/13, Vladimirska Str., 01033, Kiev, Ukraine e-mail: 2 Technische Universitat Ilmenau, Institut fur Chemistry & Biotechnoly, Postfach 100565, 986884 Ilmenau, Germany

Resume : The trend of organic thin films research toward conductivity - photovoltaic chip has allowed using the/films from ds-DNA –templated naocarbons molecular layers obtained by biotechnology. On the base of the review deals to analysis of electronic properties, photosensitivity, photoelectron moving force (PhEMF) and their stability under UV-vis irradiation for nanocarbon films with DNA molecules, we selected the thin films from silf-assembled layers of fullerene C60/C60 oxygen derivatives/ds-DNA on silicon for the detail investigations. The developing model of conductivity, and photovoltaic effect, in these layerss takes in account that C60 molecule is an acceptor of electrons. And the effect enhances with formation of C60 oxygen derivatives: 6-5 open fullerene C60 as we showed in first time [1].The evidence of self-assembling of these layers with (ds-DNA) in the nanostructured films on Si surface were obtained on the base of STM and SEM images of the films with the assembles (8-10 and 30-40 nm in diameter).The conductivity of the films was modulated by diode characteristics of fullerene C60/6-5 open fullerene C60 and C60/ ds-DNA contacts for n-type semiconductors fullerenes witch were determined by STP - tunneling spectroscopy. The discovered dynamic behavior of photosensitivity to 200-400 nm irradiation and PhMF appearance (0,25-0,37 eV) at 400-1000 nm irradiation (during 10 min - 1 h) of these films with several structures allow to consider these nanostructured layers/films as conducting/ photovoltaic chips. The examples for an application of these chips based on conductivity/photovoltaic models which have been developed for organic nanostructured thin films from C60 fullerene/ds-DNA molecular assemblies are discussed. Ref.,{1] E. Buzaneva, A. Gorchinskiy, P. Scharff, K. Risch, A. Nassiopoulou, C. Tsamis, Yu. Prilutsyy, O. Ivanuta, A. Zhugayevych, D. Kolomiyets, A. Veligura, DNA, DNA/Metal Nanoparticles, DNA/Nanocarbon and Macrocyclic Metal Complex/Fullerene Molecular Building Blocks for Nanosystems: Electronics and Sensing, In Book Frontiers of multifunctional integrated nanosystems, Eds: Eugenia Buzaneva and Peter Scharff, NATO Science Series, II-Mathematics, Physics and Chemistry–Vol 64, Kluwer Akademic Publishers, Dordrecht, 251-276, 2004.

Authors : Wiktoria Lipińska (1)*, Katarzyna Siuzdak (1), Piotr Barski (2), Agnieszka Lindstaedt (2), Katarzyna Grochowska (1)
Affiliations : (1) Centre for Plasma and Laser Engineering, The Szewalski Institute of Fluid-Flow Machinery, Fiszera 14, 80-231 Gdańsk, Poland; (2) Prochimia Surfaces, Zacisze 2, 81-823 Sopot, Poland

Resume : Nowadays, diabetes became a serious problem since everyday 400 million people face with it. Following that, extensive research works towards the enzymatic electrochemical biosensors are undertaken since those devices can selectively monitor glucose level in human body fluids. In this work, preparation method and electrochemical activity of the enzyme-functionalized Au-Ti electrode are presented. Firstly, the conductive platform was fabricated via anodization process of Ti and further chemical etching that results in regularly distributed nanodimples. Secondly, the thin Au film sputtering followed by the thermal annealing leads to the Au nanoparticles formation in the host Ti hollows revealed by scanning electron microscopy. Thirdly, the surface of obtained material was modified with glucose oxidase by the hybrid immobilization route. The first layer was the self-assembled organic monolayer composed of HSC11H22EG6OCH2COONHS chains. Then, GOx enzyme was anchored via the N-hydroxysuccinimide ligand using the cross-linking immobilization route. Basing on the cyclic voltammetry measurements performed in PBS the sensitivity of 43.926 μMcm-2mM-1 was reached within glucose concentration range of 0.006-2.345 mM while the limit of detection was as low as 5.331 μM. Additionally, the measurements in artificial sweat and saliva as well as human serum were carried out to show the performance in the real conditions. Research is financed by NCBR via LIDER/2/0003/L-8/16/NCBR/2017 grant.

Authors : Aishee Dey* (1), Preetam Guha Ray (2), Santanu Dhara (2) & Sudarsan Neogi (1)
Affiliations : (1) Department of Chemical Engineering, Indian Institute of Technology Kharagpur, India - 721302 (2) School of Medical Science and Technology, Indian Institute of Technology Kharagpur, India - 721302.

Resume : Nanosized zinc oxide is gaining importance in the biomedical field due to its commendable antibacterial efficacy. The increased specific surface area of the nanoparticles ensures enhanced particle activity, hence proving itself to be more effective than the bulk sized particles. The fluorescence property of zinc oxide arised due to defects and its use in biomedical field is recently being explored. In this work, shape controlled fluorescent zinc oxide nanoparticles was synthesized by a facile and novel wet chemical technique. Morphological and physico-chemical characterization of the nanoparticles were performed in order to affirm structural and functional aspects of the nanoparticles. Further, bactericidal activity of the 30 nm sized ZnO nanoparticles were tested against B. subtilis and E. coli. It was observed that 100 ppm conc of the nanoparticles were able to demonstrate 99% (5 log reduction) in bacterial colony size in 6 h of incubation time. Similar results were also witnessed in zone of inhibition, growth curve analysis, DNA leakage assay and live-dead assay, thus confirming bactericidal efficacy of the nanoparticles. Further, in vitro cytocompatibility was also performed with L929 fibroblast cells in order to evaluate toxicity profile of the nanomaterial. The excellent bactericidal efficacy coupled with low toxicity profile renders the fluorescently active sub-50 nm sized ZnO nanoparticles as an excellent alternative for next generation non-invasive biomedical applications. References 1. Ray, Preetam Guha, et al. "Sonication Assisted Hierarchical Decoration of Ag-NP on Zinc Oxide Nanoflower Impregnated Eggshell Membrane: Evaluation of Antibacterial Activity and in Vitro Cytocompatibility." ACS Sustainable Chemistry & Engineering 7.16 (2019): 13717-13733. 2. Alekish, Myassar, et al. "In vitro antibacterial effects of zinc oxide nanoparticles on multiple drug-resistant strains of Staphylococcus aureus and Escherichia coli: An alternative approach for antibacterial therapy of mastitis in sheep." Veterinary world 11.10 (2018): 1428.

Authors : Wenyi Hu* (1), Yuval Elani (2), and Claudia Contini (1).
Affiliations : (1) Department of Chemistry, Imperial College London, London, UK (2) Department of Chemical Engineering, Imperial College London, UK.

Resume : The current state of the art in artificial cell science relies on the construction of lipid vesicle membranes whose inherent properties, however, limit their applications particularly in sustaining cell internal biochemistry and communication between neighbors. Polymersomes are considered as ideal alternatives to liposomes because of their enhanced stability, chemical versatility, chemical and mechanical resistance. Nevertheless, the use of polymeric vesicles is restricted due to their low membrane permeability. Here, a suite of porous polymer membranes which is intrinsically permeable to molecules of defined sizes is explored as chassis for artificial cell, with the ideal functionalities of retaining specific molecules (e.g. protein and DNA) but allowing influx and efflux of small molecules (e.g. metabolites, signal molecules and mRNA). The porous vesicles are expected to form directly by self-assembly of copolymers, and the porosity is hypothesized to be controlled by varying experimental parameters during extrusion. The combinations of copolymers involve the mixtures of diblock copolymer PEG-PBD and triblock copolymer PEG-PPO-PEG with different molar ratios, and the solutions of two or more triblock copolymers PEO-PPO-PEO with different head-to-tail ratios. The porous polymer vesicle is novel in artificial cell science; thus, many potential applications can be tapped into, including for spatially-segregated biochemical reactions in polymer-based synthetic organelles.

Authors : Xiaozhen Li, Shuang Tian, Zhongming Huang, Yafang Xiao, Xiao Cui, Chun-Sing Lee
Affiliations : City University of Hong Kong

Resume : High-performance photothermal agents remain highly required for realizing efficient tumor photoelimination. However, low-photoconversion and non-biodegradability of photothermal materials seriously lead to restricted tumor suppression or unavoidable biosafety issues. In such, a major mission is focused on developing efficient and dependable agents with prominent photothermal conversion efficiency for maximized cancer photoelimination. Herein, we developed a biodegradable photothermal therapeutic (PTT) agent, π-conjugated oligomer nanoparticles (F8-PEG NPs), for highly efficient cancer theranostics. By exploiting an oligomer with excellent near-infrared (NIR) absorption, the nanoparticles show a high photothermal conversion efficiency (PCE) up to 82% surpassing those of reported inorganic and organic PTT agents. In addition, the oligomer nanoparticles show excellent photostability on one hand but also good biodegradability. The F8-PEG NPs are also demonstrated to have excellent biosafety and PTT efficacy both in vitro and in vivo. This contribution not only proposes a promising oligomer-based PTT agent, but also provides insight into developing highly efficient nanomaterials for cancer theranostics.

Authors : Xiao Cui,Yafang Xiao,Xiaozhen Li,Shuang Tian,Qi Zhao
Affiliations : Department of Chemistry, Institution Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, Address 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, P. R. China

Resume : Exploitation of new photosensitizes with sufficient oxygen-independent O2−• generation is highly desirable for overcoming hypoxia in photodynamic therapy (PDT). Herein, we report the first demonstration using a radical molecule as a new photosensitizer for hypoxic-overcoming photodynamic therapy. After self-assembling the radical molecules into nanoparticles (NPs), the NPs show good water dispersibility, good biocompatibility, board near infrared (NIR) absorption and emission at ~ 800 nm. Significantly, radical NPs remain stable in various biological solution, 100-days exposure to ambient environment, and long-term laser irradiation, which are ultrastable compared to many reported radical materials. Efficient 1O2 and O2−• generation and cytotoxicity were observed upon NIR irradiation. More importantly, even under hypoxia condition, sufficient oxygen-independent O2−• generation and cytotoxicity were observed addressing the most important hurdle for successful PDT in oxygen-deficient tumor microenvironment. This work demonstrates that stable radical molecules could have interesting properties suitable for novel biomedical applications.

Authors : Yafang XIAO, Xiao CUI, Shuang TIAN, Xiaozhen LI, Qi ZHAO.
Affiliations : Department of Chemistry Institution Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, Address 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, P. R. China

Resume : Rational manipulation of energy utilization from excited-state radiation of theranostic agents with donor-acceptor structure is relatively unexplored. Herein, we present an effective strategy to tune the exciton dynamics of radiative excited state decay for augmenting two-photon nanotheranostics. As a proof of concept, two thermally activated delayed fluorescence (TADF) molecules with different electron-donating segments are engineered, which possess donor-acceptor structures and strong emissions in the deep-red region with aggregation-induced emission characteristics. Molecular simulations demonstrate that change of the electron-donating sections could effectively regulate the singlet-triplet energy gap and oscillator strength, which promises efficient energy flow. Two-photon laser with great permeability is used to excite TADF NPs to perform as theranostic agents with singlet oxygen generation and fluorescent imaging. These unique performances enable the proposed TADF emitters to exhibit tailored balances between two-photon singlet oxygen generation and fluorescence emission. This result demonstrates that TADF emitters can be rationally designed as superior candidates for nanotheranostics agents by the custom controlling exciton dynamics.

Authors : Shi Mo (1), Paul K. Chu, *(1), Kaiwei Tang (1), Babak Mehrjou (1), Guomin Wang (1), Huaiyu Wang (2).
Affiliations : (1) Department of Physics, City University of Hong Kong, Hong Kong, China; (2) The Shenzhen Institutes of Advanced Technology, The Chinese Academy of Science (CAS), China.

Resume : Sheet-like structures such as nanoflake and nanolamellae are of great interest in biomedical engineering, because the sharp edge or large surface area can affect the bacterial and cellular behaviors on attaching to these structures. The nanolamellar arrays can be readily constructed on the surface of polyetheretherketone (PEEK) through one-step plasma treatment, showing a re-crystallization and time-dependent growth process. The gap, thickness and size of the nanolamellar array are controlled by adjusting the volume of plasma injection and the treatment time, which in turn affect the antibacterial activity and the growth of osteoblasts. The sharp edge and dimensional parameters of the nanolamellar arrays contribute to the antibacterial activity. However, due to the large size of the osteoblast and the stiffness of the cell membrane, the osteoblasts can grow on the arrays, which is affected by the dimension of the arrays. The improved osteoblast proliferation also renders it desirable osteointegration activity. The overall results in this work offer insights into the antibacterial behavior and the osteogenesis of the nanolamellar arrays, thus assuring this type of structure better application in biomedical engineering.

Authors : Ting-Yu Lu, Ching-Cheng Tsai, and Jiashing Yu*
Affiliations : Department of Chemical Engineering, National Taiwan University

Resume : Human adipose-derived stem cells (hASCs) can differentiate into multiple lineages and be harvested abundantly. However, the expression of pluripotency markers, which is important for the renewal and differentiation capabilities of hASCs, decreases during monolayer culture. Increasing evidence has proven that cells aggregated to form cell spheroids in 3D cell cultures better mimic the in vivo microenvironment and can enhance the expression of stemness markers. In this study, first, uniform hASC spheroids were formed by seeding cells in agarose microwell plates, and the size of the spheroids could be adjusted. Most importantly, the stemness expression of the spheroids increased significantly. Additionally, we utilized microbial transglutaminase (mTG), which is an enzyme that exhibits highly specific activity over a wide range of temperature and pH, to crosslink gelatin. The enzymatic crosslinking reaction is milder than physical and chemical methods, which may lead to cell death. The properties of the gelatin/mTG hydrogel were evaluated in detail. In addition, the spheroids were encapsulated in the 3D hydrogel successfully. The results showed that the hydrogel has low toxicity to the cells, which significantly proliferated in the 3D hydrogel. Moreover, the analysis of the differentiation potential indicated that the cell spheroids in the 3D hydrogel exhibited good activity, especially adipogenesis and chondrogenesis, compared to the cell suspension group. Furthermore, the in vivo data confirmed the excellent injectability and biocompatibility of the 3D hydrogel. Second, we demonstrated that cell spheroids entrapped in gelatin hydrogel can accelerate wound healing. To evaluate the in vivo effects of these spheroids/hydrogel system, we applied hASC single cells/hydrogel and hASC spheroids/hydrogel in a designed rat skin repair model. Results showed that skin wounds treated with hASC spheroids hydrogel system had faster-wound closure and a significantly higher ratio of angiogenesis. Based on the in vitro and in vivo results, the self‐assembled hASC spheroids hydrogel system may be a promising cellular source for skin tissue engineering and wound regeneration.

Authors : Seungje Lee*(1), Minji Ko(1), Soomin Ahn(1), Seo Yeon Shin(1), Gang Yeol Yoo(2), Woong Kim(2) and Young Rag Do(1)
Affiliations : (1) Department of Chemistry, KOOKMIN University, Seoul, Republic of Korea, (2) Department of Materials Science and Engineering, Korea University

Resume : Multifunctional anti-reflective (AR) SiO2 nano-rod structures were fabricated on glass on an ITO substrate using nano-spheres in a hexagonal-closed-packing array in an attempt to improve the efficiency of light extraction. Compared to the reflection of flat ITO/glass substrates, surface reflection of AR patterned ITO/glass decreases with gradual change of refractive index, resulting in an increase in the amount of transmitted light. SiO2 nano rod structures were fabricated by a combination of polystyrene (PS)-based nano-sphere lithography (NSL) and reactive ion etching (RIE). Dimensions of height and diameter can be adjusted by selective gas etching process. O2, CHF3, and Ar gas were selected to fabricate the SiO2 nano structure. Finally, moth-eye patterned AR SiO2 nano structures were fabricated. The measurement results for our nanostructure (diameter: 150 nm, height: 400 nm) showed a 2% reflectance reduction in the 380~780 nm range compared to reflectance of flat ITO/glass substrate. Due to the deposition of SiO2 layer by Plasma Enhanced Chemical Vapor Deposition (PECVD), super hydrophilic nano patterns were obtained. To obtain an anti-fogging effect, we performed self-assembled monolayers (SAMs) treatment by applying perfluoro siloxane (PFS) on AR patterned SiO2 nanostructure. A field emission scanning electron microscope (FE-SEM) was used to investigate the morphology of the AR-patterned SiO2 nanostructure. Hydrophobicity was measured by contact angle measurement.

Authors : Miryam Criado-Gonzalez1,2; Alejandro Hernandez-Sosa3; Maria Rosa Aguilar3,4; Luis Rojo3,4; Fouzia Boulmedais1; Rebeca Hernández3
Affiliations : 1. Université de Strasbourg, CNRS, Institut Charles Sadron, Strasbourg, France 2. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, Strasbourg, France 3. Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Madrid, Spain 4. Biomedical Research Networking Center in Biomaterials, Bioengineering and Nanomedicine, CIBER-BBN, Monforte de Lemos, 5. 28029 Madrid, Spain

Resume : We report on the development of interpenetrating polymer networks (IPN) for specific application in osteochondral regeneration based on the employment of Localized Enzyme Assisted Self-Assembly (LEASA) systems on hydroxyapatite nanoparticles (HA-NPs) as building blocks for the construction of 3D peptide based hydrogels and their combination with alginate hydrogels crosslinked with bioactive cations: Ca2+, Zn2+ and Sr2+. Silica nanoparticles have been recently reported to control the self-assembly of a peptide based hydrogel onto their surface by enzymatic transformation of non-interacting peptides into hydrogelators. To the best of our knowledge, this is the first time that HA nanoparticles are employed as templates for peptide gelation. The combination of alginate and a peptide network as an IPN gives rise to a bioactive material able to be printed, with modulated mechanical properties depending on the concentration of HA nanoparticles and the crosslinker: polymer ratios. As a first step, UV-vis experiments demonstrated the successful adsorption of alkaline phosphatase (ALP) onto HA-NPs. A gel was obtained after 24 hours when ALP-HA-NPs were put in contact with a solution of Fmoc-FFpY peptide. Then, the formation of the IPN was ascertained through morphological and rheological measurements. The determination of the yield stress, thixotropic properties and gelation time allowed to assess the suitability of the proposed material for bioprinting.

Authors : Dr., Docent Oleksandr .Ivanyuta
Affiliations : Taras Shevchenko National University of Kyiv,64/13, Volodymyrska Str., Kyiv, 01601, Ukraine

Resume : Excessive microwave exposure in the early 1970s led to injuries and fatal accidents. The higher the power of microwave radiation, the greater the pathological changes in nerve cells. The study aims to investigate the effect of microwave radiation on botanic and animal DNA. [1] DNA of lily flowers and cattle were selected for this study. DNA were exposed for 1 min a day 10 h for 15 days at 1 - 30 GHz frequency (power density, 0.1 µW / cm (2)). The whole-body specific absorption rate was estimated to be 5 µW / kg. Exposure took place in a ventilated resonator surface wave placed in a rectangular waveguide. After completion of the exposure period, DNA were samples conductivity was measured. Experiments were performed in a blind manner and repeated. After completion of the exposure period, DNA were samples conductivity was measured. Experiments were performed in a blind manner and repeated. A significant decrease conductivity was after 9 days of exposure for DNA lily flowers as compared with DNA cattle after 14 days exposed. The study concludes that a reduction in conductivity or an increase in sample volume may cause significant damage in brain due to chronic exposure of these radiations. I report on a new method of electrodynamic modeling of the complex biological objects. The method is based on the computer experiment realized with CAD systems. One-way analysis of variance method was adopted for statistical analysis. Simulations have with CAD systems shown that microwaves in the frequency range from 1 to 30 GHz can penetrate the cranium and that <20% of them can penetrate the deep brain, where they can penetrate <1-4 cm into the brain. These biomarkers clearly indicate possible health implications of such exposures. The great attention is given to the influence of microwave electromagnetic field on a human body, first of all, on a human head. 1. Kesari KK, Kumar S, Behari J. Pathophysiology of microwave radiation: Effect on rat brain. Appl Biochem Biotechnol. 2012;166:379–388

Authors : Susloparova, A.* (1), Mahdi, G. (1), Guérin, D. (1), Halliez, S. (2), Colin, M. (2), Buée, L. (2), Coffinier, Y. (1), Dargent, T. (1), Alibart, F.(1,3), Pecqueur S. (1).
Affiliations : (1) Univ. Lille, CNRS, Centrale Lille, Yncréa ISEN, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000 Lille, France (2) Université de Lille, Univ. Lille, Inserm, CHU-Lille, Jean-Pierre Aubert Research Centre (JPARC, UMR - S 1172), 59045 Lille, France (3) Laboratoire Nanotechnologies & Nanosystèmes (LN2), CNRS, Université de Sherbrooke, J1X0A5, Sherbrooke, Canada.

Resume : One great challenge for bio-sensing is to ensure that detection/transduction of biochemically rich systems is fully mastered to reliably record relevant information descriptors. For neuro-sensing with microelectrode arrays (MEAs), signal transduction lies at the interface between extracellular ionic currents and the electrodes. By chemically/morphologically engineering electrode materials’ interface to ensure the best electrochemical surface impedance, the aim is to find the right materials that detect ionic signals from neurons and transduce them into electronic signals with the lowest information loss. In this direction, we show how to lower the surface impedance of microelectrodes by electropolymerization of synthesized thiophene-based monomers, functionalized for higher cell biocompatibility and higher electrochemical performances. By in-situ monitoring of impedance change with voltage-ramped impedance spectroscopy, we systematically screened electrical and chemical deposition conditions to control the materials’ morphology (confirmed by atomic force and electron microscopy) reaching lower impedances than commercial MEAs and comparable to state-of-the art spin-coated polymers. With the presented preliminary biocompatibility tests, we aim to show that unconventional deposition processes can access to wider ranges of materials’ chemistry and morphology that cope with biological-constraints/electrical-performances to unlock future emerging bioelectronics technologies.

Authors : Miguel A. Jimenez-Munoz, Kenny Malpartida-Cardenas, Nicolas Moser, Pantelis Georgiou, Jesus Rodriguez-Manzano
Affiliations : Centre for Bio-Inspired Technology, Department of Electrical and Electronic Engineering, Imperial College London, London, UK

Resume : According to the WHO, Malaria is one of the most severe infectious diseases in the world, affecting almost half of the population. Thus, early diagnosis becomes critical. This disease is caused by the Plasmodium parasite whose main human-infective species are: P. Falciparum (Pf), P. Vivax (Pv), P. Ovale (Po), P. Knowlesi (Pk) and P. Malariae (Pm). Point-of-Care (PoC) systems promise to be a fast and costless alternative to current diagnosis methods, suitable for resource limited settings. Nevertheless, they still present some limitations such as low detection limit or the need of expensive optical equipment. More importantly, most of these PoC systems only focus on the detection of pan-Plasmodium, or Pf, leaving infections caused by other species undiagnosed. Increasing infections of Pv, Pk or Po in countries such as India or Malaysia have been recently reported, urging the development of new PoC devices for their detection. In previous work we have shown a novel Lab-on-a-Chip (LoC) electrochemical biosensor based on real-time LAMP and Ion-Sensitive Field Electrode transistors (ISFET) able to detect Pf. In this work, we propose to extend this system to the detection of other Plasmodium species. This methodology allow us to combine the accuracy of nucleic acid amplification with sensitive and unlabeled detection provided by the ISFET sensors. Furthermore, this LoC technology is promising for multiplexing, allowing the detection of several pathogens at once, ideal for PoC applications.

Authors : M. Chetyrkina (1), F. Talalaev (1,2), S. Kostyuk (3) and P. Troshin (1,2).
Affiliations : (1) Skolkovo Institute of Science and Technology, Russia (2) Institute for Problems of Chemical Physics of Russian Academy of Sciences (IPCP RAS), Russia (3) Research Centre for Medical Genetics (RCMG), Russia

Resume : The preventive and personalized medicine strategy for disease treatment stands apart from traditional medicine therapy. Nowadays, non-invasive monitoring and diagnostic systems based on wearable electronics and e-skin are considered as crucially important for implementation of smart healthcare technologies. However, materials for such devices should fulfill specific requirements, e.g. have a good combination of charge-transport properties, mechanical flexibility, and biocompatibility. In this work, we explored the biological effects of a series of different organic semiconductors on in vitro living system. Human embryo lung fibroblasts were incubated at standard conditions with thin films of studied materials. Cells were fixed at different time points in order to find out acute and long-term effects on fibroblasts (30 hours, 96 hours, 14 days). Flow cytometry and immunofluorescence microscopy techniques were performed with antibodies to H2A histone family member X and 8-oxoguanine for DNA damages evaluation. The occurred changes in cell metabolism were detected by real-time polymerase chain reaction, which was carried out with primers to the genes involved in apoptosis regulation and oxidative stress in cells. Results of our study demonstrated that some of the studied organic semiconductors represent biocompatible materials suitable for wearableand on-skin electronics.

Authors : Zhuyun Li, Benhui Hu, Eugene V. Makeyev, Xiaodong Chen
Affiliations : Dr. Z. Li, Dr. B. Hu, Prof. X. Chen Innovative Center for Flexible Devices (iFLEX) School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798, Singapore Prof. E. V. Makeyev Centre for Developmental Neurobiology King’s College London New Hunt’s House London SE1 1UL, UK

Resume : Cellular signalling through diffusible factors and direct cell-to-cell contacts are known to play a critical role in controlling cell viability. To understand how mechanical neuron-neuron contact affects neural survival is essential for study brain development. However, tools to systematically manipulate inter-neuron connection rarely occurs in previous studies. Herein, we developed a strategy to impose stereotypic interneuronal spacing and connectivity using topology micropatterns. With the artificial surface, we found that increased inter-neuron connectivity promotes the survival of neurons by activating neuron survival pathways. Overall, our approach helped to pave the way to improve primary neuron cell culture methodology, and more importantly, enable us to manipulate neuronal survival simply by controlling the connectivity between neurons.

Authors : Ting-Ying Wu, Zhen-Yu Guan, Chih-Yu Wu and Hsien-Yeh Chen*
Affiliations : Department of Chemical Engineering, National Taiwan University

Resume : The 3-dimensional(3D) porous materials or scaffold have been widely applied to the different fields. Here, we report an innovative manufacturing process for the fabrication of 3D porous structures containing any ingredients even the living cells via the chemical vapor deposition (CVD). This report indicates that the scaffold via sublimation/deposition process could encapsulate any substances, including living cells, growth factors and functional guiding molecules, to form a mono- or multi-functional scaffold and performed its biological function. According to the results, the survival rate of the cell-loaded scaffold via sublimation/deposition process was 80.8±5.3%. The proliferation and osteogenesis activities of MC3T3-E1 loaded in scaffold composited with ingredients, fibroblast growth factor (FGF-2) and bone morphogenetic protein (BMP-2), respectively, could be enhanced, comparing with cell-loaded scaffold without ingredients. Otherwise, osteogenesis and adipogenesis of human adipose-derived stem cells (hASCs) encapsulated in scaffold composited with PRP and cultured with induction medium performed better than the others group without PRP or induction medium. Moreover, according to the neurogenesis test, neurite length of pheochromocytoma 12 cell line (PC12) loaded in scaffold composited with ingredients, PEDOT:PSS and PRP, was 115.3 ± 26.8 μm which is the longest in different groups and tendency of neurogenesis activity were also as same as neurite length. Based on these result, the ingredients could be successfully loaded in the same Trojan scaffold, and also perform their individual functions. This novel approach and scaffold mentioned here provide another way for fabricating multifunctional scaffold with potentially broad applicability.

Authors : Dongkyu Kang * (1), (2), Jaechul Pyun (2) & Joonseok Lee (1), (3).
Affiliations : (1) Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, South Korea (2) Department of Material Science and Engineering, Yonsei University, Seodaemun-gu Seoul 120-749, South Korea (3) Division of Nano and Information Technology, KIST School, Korea University of Science and Technology (UST), Seoul * lead presenter

Resume : Upconversion nanoparticles (UCNPs) are commonly used as energy donor via a LRET (luminescence resonance energy transfer) in biological applications. In contrast to classic RET donors such as gold nanoparticles, quantum dots, UCNPs can emit near-infrared (NIR) upon the NIR excitation, which provides reduced signal-to-noise ratio due to the strong penetration and less autofluorescence in NIR region called diagnostic window. Here, we report NIR-to-NIR signal based LRET system for detection of progesterone, a natural steroid female sex hormone. We synthesized NaYF4@NaYF4:Yb,Tm@NaYF4 inert-core/active-shell/inert-shell UCNPs as LRET donor. Progesterone-HRP-IRdye and progesterone-BSA-IRdye were used as LRET acceptors with different size for efficient LRET from the UCNPs due to exact spectrum match of the UCNP’s emission and IRdye QC-1’s absorption. Anti-progesterone antibody was conjugated on the surface of water-soluble UCNPs. Progesterone was detected in NIR-to-NIR signal based LRET system via competitive immunoassay. The limitation of detection for progesterone was determined to be 1.36 pg/ml in 10-fold diluted human serum samples. We believe that the LRET system have considerable diagnostic potential, being background-free and able to rapidly detect biomarkers.

Authors : Pingqiang Cai, Zhuyun Li, Ela Sachyani Keneth, Luying Wang, Changjin Wan, Ying Jiang, Benhui Hu, Yun-Long Wu, Shutao Wang, Chwee Teck Lim, Eugene V. Makeyev, Shlomo Magdassi,* and Xiaodong Chen*
Affiliations : Prof. X. Chen, Dr. P. Cai, Dr. Z. Li, Dr. C. Wan, Dr. B. Hu, Dr. Y.-L. Wu, Y. Jiang Innovative Center for Flexible Devices (iFLEX),School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore Prof. S. Magdassi, E. S. Keneth Institute of Chemistry, Centre for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. Prof. S. Wang, Dr. L. Wang CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing, 100190, P. R. China. Prof. C. T. Lim Mechanobiology Institute, Department of Biomedical Engineering, National University of Singapore, 5A Engineering Drive 1, Singapore 117411. Prof. E. V. Makeyev Centre for Developmental Neurobiology, King's College London, New Hunt's House, London SE1 1UL, UK.

Resume : Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems led current studies to approximate it simply as an in-plane stress. To evaluate possible contribution of force vectors acting in other directions, we reconstituted double epithelium in a 3D-printed GeminiChip containing a sessile and a pendant channel. Intriguingly, the sessile epithelia were prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favored live cell delamination. Transcriptome analyses showed upregulation of RhoA, BMP2 and hypoxia signaling genes in the pendant epithelium, consistent with the onset of an epithelial-mesenchymal transition program. HepG2 microtumor spheroids also displayed differential spreading patterns in the sessile and pendant configuration. Using multilayered GeminiChip, our results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer.

Authors : Jing Yu, Chen Xiaodong
Affiliations : Nanyang Technological University

Resume : Biological processes such as morphogenesis, angiogenesis or embryogenesis involves group of cells coordinating their movements collectively to achieve specific functions, in a constricted fashion. Unlike single cell, collective cellular motions, which involve intercellular coordination, are much more complex and largely remain mysterious. Variances in environment could easily lead to changes in cell behaviors; factors such as chemical, physical or mechanical signals are common regulators in guiding cell migration. Among these factors, topological cue is a critical, yet easily neglected factor. Different from most benchside scenarios, in which migrations are allowed on infinite surfaces, the physiological microenvironment of cellular migration is usually confined. Here we built fibronectin patterns on polyacrylamide substrate to study confined cell migration. We showed that motion of cell cohort could be modeled through topological cues and confinement. Madin-Darby Canine Kidney (MDCK) cell line is used as an epithelial model to describe a form of oscillation phenomenon under various circular confinement, which amplitude and frequency vary with both the size of constrain as well as regions in the confined monolayer. Through spatio-temporal force analysis, increased intercellular tension and cell-substrate traction are observed with increasing degree of confinement. Together with confocal microscopy of actin cytoskeleton and average orientation field mapping on the cell sheet, a possible fluid-to-solid transition could attribute to this interesting collective cell motion.

Authors : Eunyoung Jeon(1)*, Joonseok Lee(1)
Affiliations : (1) Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Division of Nano and Information Technology, KIST School, University of Science and Technology (UST), Seoul, 02792, Korea

Resume : Conventional drug-eluting stents (DESs), biomedical implants, mainly use polymers or laser etching to load drugs. However, there are limitations in that polymers cause side effects such as late-thrombosis in the body and laser etching is complicated and expensive. Herein, we propose a porous nanostructured silica film for polymer-free DESs platform, which is synthesized by a sol-gel process using a catalyst, a silicon source, and structure-directing agents. As the structure-directing agents form micelles and grow on the surface, the porous nanostructured silica film is formed and has tunable pore sizes and channel lengths by adjusting reagents, molar ratios and reaction time. This suggests that a large amount of materials can be loaded, from large molecular weight molecules to small ones, and the films can be easily formed on the surface of commercial bare-metal stent. In addition, it is expected to be physically stable in the actual applications through pico-indentation experiment, expansion simulation and SEM, which evaluates the physical characteristics such as young's modulus (85.26 GPa) and hardness (2.69 GPa). Moreover, surface functionalization into hydrophobic with silane coupling agents (Octyltrimethoxysilane, Hexadecyltrimethoxysilane) allow the hydrophobic drugs to be loaded without polymers. The porous nanostructured silica film may represent an attractive platform that can be used in many other areas as well as polymer-free DESs.

Authors : Petruta Preda1,3*, Marioara Avram1,2, Diana Stan2, Bogdan Mincu2, Anton Ficai3
Affiliations : 1 National Institute for Research and Development in Microtechnologies - IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190, Bucharest, Romania 2 DDS Diagnostic SRL, 7 Vlad Judetul Street, Bucharest, Romania 3 Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 1-7 Gh. Polizu, 011061, Bucharest, Romania

Resume : Bionanocomposite films based of polycaprolactone (PCL) and fish collagen nanoparticles embedded with sea buckthorn oil were prepared by the solvent evaporation method at room temperature conditions. These materials were performed in order to be used as promising candidates in medical applications like regeneration of skin wounds and not only (surgical suture and prosthetic devices) by improvement the proliferation of fibroblast cells properties. The following raw materials were used for obtaining the polymeric composites: polycaprolactone (Sigma-Aldrich, Mw = 45,000); fish collagen extracted in the laboratory from fish waste; pure cyclohexanone (Sigma-Aldrich); acetic acid (Sigma-Aldrich) and sea buckthorn oil. Both polymeric films developed as well as collagen and sea buckthorn oil respectively have been physico-chemically and biologically characterized (FT-IR, SEM, UV-vis, EDX, HPLC, SDS–PAGE, WCA/water contact angle and fibroblast cell cultures study). Experimental results suggest that polycaprolactone films with collagen and sea buckthorn could be used for medical applications, when additional studies are required. Keywords: polymeric composites, skin wounds, fibroblast cell cultures ACKNOWLEDGEMENTS The work of PETRUTA PREDA has been funded by the Operational Programme Human Capital of the Ministry of European Funds through the Financial Agreement 51668/09.07.2019, SMIS code 124705. This work was supported by the grant COP A 1.2.3., ID: P_40_197/2016.

Authors : Jin Gu Kang, N. Ashwin K. Bharadwaj, Gaurav Chaudhary, Ashesh Ghosh, Marta C.Hatzell, Kenneth S. Schweizer, Randy H. Ewoldt, Paul V. Braun
Affiliations : Korea Institute of Science and Technology; University of Illinois at Urbana-Champaign

Resume : The study of a composite system made of semi-flexible polymeric networks combined with colloidal particles has remained in its infancy despite its ubiquitous nature in the biological space. By imparting functionalities into colloids or polymer networks, reconfigurable and active materials system that dynamically respond to external stimuli can be realized. Here, we demonstrate the 3D hydrogel composites consisting of semi-flexible fibrin networks and colloids. We develop the methods of integration of colloidal particles into fibrin networks and observe the structure of the resulting composite hydrogels. We characterize the mechanical properties by measuring oscillatory shear elastic moduli at the linear and non-linear regimes. The relation between the structure and the mechanical properties are discussed. Furthermore, we synthesize thermo-responsive hard-sphere colloids and soft-microgel colloids via atom-transfer-radical-polymerization and surfactant-free emulsion polymerization respectively. We successfully incorporate these into fibrin networks using the developed method. The changes in structure and dynamics upon the temperature change are observed and the origin of this switchable property is discussed.

Authors : A.F. Bonciu, S. Iosub, M. Filipescu, V. Dinca* and M. Dinescu
Affiliations : A.F. Bonciu 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania 3) University of Bucharest, Faculty of Physics, RO 077125, Magurele, Romania S. Iosub 2) Institute of Biochemistry of the Romanian Academy, 060031 Bucharest, Romania M. Filipescu 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania V. Dinca 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania M. Dinescu 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania

Resume : In the last few years, Ceria (CeO2) nanostructures are thought to improve the biointerface mechanical properties, but also to stimulate the regenerating tissue biochemical activity by neutralizing the oxidative stress and stimulating cells proliferation. Nevertheless, there is a lack of information of mamallian cells on Ceria coatings, especially with well-defined nano and microstructured surface. Within this context, we present for the first time new designed pyramid shaped nanostructured ceria films obtained by Pulsed Laser Deposition (PLD), targeting the modulated response of SaOs-2 bone cells. The nanostructured surfaces obtained by PLD revealed shapes from the quasipyramidal, with rounded edges and dimensions of 90-120 nm, to the sharp edges and dimensions up to 350 nm, obtained by varying the number of pulses. The shapes and sizes of the nanostructures influenced both wettability and cellular behaviour. Nevertheless, the influence of pyramidal shaped ceria on the in vitro biological performance of SaOs-2 cells used as bone cell model was demonstrated by the differences in early adhesion and distribution of phenotype SaOs-2 cells, onto the ceria surface. In conclusion, different nanostructured ceria films obtained by PLD influenced especially the early response of osteosarcoma cell and are good candidates to improve the performance of orthopedic implants. In perspective, different cells lines response will be analysed toward establishing an osteogenic response. Acknowledgement This work was supported by the National Authority for Research and Innovation in the frame of the Nucleus Program and grants of the Romanian Ministry of Research and Innovation,CCCDI-UEFISCDI, project numbers PN-III-P1-1.2-PCCDI-2017- 0637 (MultiMonD2).

Authors : Ivanyuta O.,1 Gogotsi H.,1 Buzaneva E.,1 Ritter U.,2 Scharff P.,2
Affiliations : 1 National Taras Shevchenko University of Kyiv, Ukraine; 2 TU of Ilmenau, Institute for Chemistry and Biotechnology, Germany E-mail:

Resume : The characterization of the protein amino-acids in natural cells and mimetic medias is one of great value in the amino-acid studies. Using results of investigations for intermolecular interaction between the C60 and ds-, ss- DNA sequences [1], predictive modeling of a interface structures for the fullerol (immobilized by –OH groups fullerene C60) molecules in a cluster and this molecule at an interface with metal ion (Cu or Ag ) complex having coordinative bond to selected amino-acid (glycine, alanine, methionine, histidine) molecules [2,3] has been realized. This modeling should derive interface properties from the chemical functionalities of the constituents, while operating stimuli- response molecular nanostructures in aqueous solutions with amino-acid molecules. Modeling was carried out taking in account that these interfaces organization may increase the photoactivity and the biocompatibility to this carbon molecule counterpart. The interface designing concept as the base for the fullerol molecule in a cluster sensor and molecular ion metal complex sensor development has been discussed. Based on well established rationale of interface organization in biological macromolecules (nucleic acids, proteins), driven by non-covalent interactions (hydrogen bonding, hydrophobic effect and electrostatic interaction) in living cells, we have plan to select biomolecules for non-covalent interaction with a core of the fullerol molecule and a molecule of organic ligand in ion metal complex – amino acids. Then molecular interface organization in solutions can be revealed by photospectroscopy. From experimental results specific interface formation between [Сuх(5-amtetrazole)у]2 complex and histidine molecule for organic specially synthesis ligand with the same coordination nature of this amino-acid molecule has been determined. A special structure of a ligand and histidine molecules gives more coordination geometry to molecular complexes, determines specific interface formation in this supramolecule.Selective coordination of such supramolecule in solutions with the histidine molecules (pH 2.7 – 12.9) was confirmed by absorbance spectra with the intensive band (600 800 nm) and it is assigned to the d – d transition of Cu. Maximum position shifted from 753 to 615 nm after adding the histidine was demonstrated. The histidine concentration effect for the solutions with the fullerol molecular clusters and the functionalized by Cu –azole ligand complex fullerol molecules was also determined. The next step is the development of controlled by light excitation interface between the fullerol, selected ion metal complex and many protein-acid molecules organization for their photo-spectroscopy recognition and single molecular biosensor controlled design. References: 1. O. Kysil, I.Sporysh, E.Buzaneva, T. Erb, G.Gobsch, U.Ritter, P.Scharff, Mat. Sc. and Eng. B169/1-3 (2010) 85. 2. O. A. Bondar, L. V. Lukashuk, A. B. Lysenko, H. Krautscheid, E. B. Rusanov, A. N. Chernega, K V. Domasevitch, Cryst. Eng. Comm, 10 (2008), 1216. 3. J. G. Mesu; T. Visser; F. Soulimani; E. E. van Faassen; P. de Peinder; A. M. Beale; B. M. Weckhuysen, Inorg. Chem., 45/5 (2006) 1960.

Authors : Gryn D.V., Naumenko A.P., Gubanov V.O.
Affiliations : Taras Shevchenko National University of Kyiv

Resume : Cisplatin (cis-Pt) is a complex of platinum which widely used as an anticancer drug. Transplatin (trans-Pt) has the same chemical formula, but different positions of atoms. Cisplatin connects to DNA in cancer cells and stops DNA replication, namely making cross-links between guanine bases. Transplatin is less reactive to DNA but also demonstrate cytotoxicity effect. In this work we make an attempt to connect the differences in the biological activity of mentioned structures with the data obtained by optical spectroscopy methods. The UV-vis spectra as well as fluorescence and phosphorescence spectra of water solutions of cis-Pt and trans-Pt were investigated at temperatures 300 and 77 K. Quantum chemical calculations were used for correct interpretation of obtained results. We also characterized these compounds using such powerful method as Raman spectroscopy.

Authors : Alkmini D. Negka, Panagiota Koralli, Maria Goulielmaki, Lida Evmorfia Vagiaki, Aristea Pavlou, Giannis N. Antoniou, Panagiotis E. Keivanidis, Dimitris Moschovas, Apostolos Avgeropoulos, Aristotelis Xenakis, Vassilis Zoumpourlis, Antonia Dimitrakopoulou-Strauss, Vasilis G. Gregoriou, Christos L. Chochos
Affiliations : Clinical Cooperation Unit Nuclear Medicine, German Cancer Research Center, 69120 Heidelberg, Germany;Institute of Chemical Biology, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, Athens, 11635, Greece; Department of Mechanical Engineering and Materials Science and Engineering, Cyprus University of Technology, Limassol 3041, Cyprus;Department of Materials Science Engineering, University of Ioannina, Ioannina 45110, Greece;National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, Athens, 11635, Greece

Resume : To date, the therapeutic properties of π-conjugated polymers especially in the form of aqueous nanoparticles, the so-called conjugated polymer nanoparticles (CPNs), have been verified merely upon laser irradiation via the well-established photodynamic and photothermal therapies. In this contribution, it is manifested for the first time the natural anticancer properties of single CPNs. This has been accomplished by studying the cell viability of new CPNs in physiological human umbilical cord mesenchymal stem cells (WJ-MSC), human colorectal carcinoma cell line (HCT116) and metastatic human melanoma cell line (WM164). The discovery of natural anticancer properties in conjugated polymers can form a new frontier in nano-biotechnology and nanomedicine and represent a new direction that is orthogonal to the more established areas of conjugated polymers for optoelectronic devices and conjugated polyelectrolyte biosensors. The combined natural anticancer and bioimaging properties of CPNs point to a new strategy for obtaining novel organic nanomaterials for cancer theranostics.

Authors : Panagiota Koralli, Alkmini Negka, Lida Evmorfia Vagiaki, Antonia Dimitrakopoulou-Strauss, Vasilis G. Gregoriou, Christos L. Chochos
Affiliations : National Hellenic Research Foundation, Institute of Chemical Biology, Athens, Greece; German Cancer Research Center (DKFZ), Clinical Cooperation Unit Nuclear Medicin, Heidelberg, Germany; National Hellenic Research Foundation, Athens, Greece

Resume : Low-bandgap aqueous conjugated polymer nanoparticles (CPNs) have been prepared and applied in biological applications, especially in bio-imaging, due to their unique optoelectronic properties.In this study, we successfully synthesized a series of conjugated polymers combining thiophene as the electron donating and quinoxaline as the electron deficient (TQs) with varying the number of fluoro atoms in the repeat unit. Water-soluble nanoparticles formatted by two different methods (nanoprecipitation and encapsulation), supporting their potential use as low-bandgap fluorescent probes for bio-imaging.The DLS measurements indicate that all CPNs in aqueous suspension have a unimodal size distribution less than 80nm.TEM & SEM measurements showed that the CPNs have spherical shape.The optical properties of the CPNs show light absorption and fluorescence emission in the UV-Vis region,tunable depending on the structure, the position and the number of fluoro atoms on the polymer backbone.It is revealed a significant reduction of the fluorescence quantum yield in aqueous media derived from severe aggregation and charge transfer induced fluorescence quenching, which can be controlled with proper rational design on the polymer backbone. Moreover, the on-going tests for cellular cytotoxicity and the ability of the obtained probes to image cancer cells are of great importance.Overall, CP dots introduce a new generation of fluorescent probes that appear to be the quintessential contrast agents

Authors : H.Gogotsi(a),,O.Ivanyuta(a),,E.Buzaneva(a),U.Ritter(b),P.Scharff(b)
Affiliations : (a)National Taras Shevchenko University of Kyiv,, 64, Volodymirska Str., 01601 Kyiv, Ukraine, (b)TU Ilmenau, Institut für Chemie and Biotechnology, Postfach 100565, 98684 Ilmenau, Germany.

Resume : The design and optical characterization of the protein amino - acids is key for understanding of the amino-acids molecules behaviour as basis sequences of biological macromolecules ( proteins) and their using as photoactive molecules, for example, in an advanced photovoltaic systems for an energy sources [1]. Therefore, we have start to design amino-acids molecules and study classic models for light excited electron transition through LUMO-HOMO band gap for the molecules and discuss ones using theories for LUMO-HOMO band gap formation for these molecules having different confirmations [2]. Then we selected amino-acid molecule for studies by the PL spectroscopy take in account that can use the laser with 408 nm wavelength (3.3 eV) for experiments. The cysteine PL excited by the laser with wavelength 408 nm( 3.3 eV) is characterized by the band in 1.5- 2.7 eV range. This band included of subbands, which are centered at 2.04, 2.24 and 2.4 eV. The present of subbands are explained by the dependence on the conformation of the molecule geometry in the cysteine layer of the HOMO-LUMO value as it is found due to compare of these subbands with calculations of electronic excitations of the cysteine conformers [2]. Therefore it is possible to use light excited electron transitions in the cysteine layer with different molecule geometry in photonic systems. 1. Koo, Suk Tai Chang, Joseph M. Slocik, Rajesh R. Naik and Orlin D. Velev, Aqueous soft matter based photovoltaic devices, J. Mater. Chem., 2011, Advanced Article.;2.R. Maul, M. Preuss, F. Ortmann, K. Hannewald, and F. Bechstedt, Electronic Excitations of Glycine, Alanine, and Cysteine Conformers from First-Principles Calculations, J. Phys. Chem. A 2007, 111, p.4370-4377.

Authors : Krasimir Koev*, Nikolaj Donkov, Hristo Naidenski, Vesslin Kussovski, Timerfayaz Nurgaliev, Latchezar Avramov
Affiliations : Assoc. prof. M.D. PhD Kr. Koev* Department of ophthalmology, Medical University 8 Byalo More str., Sofia, Bulgaria, E-mail: Assoc. prof. M.D. PhD Kr. Koev, Assoc. Prof. Dr. N. Donkov, Prof. D.Sc. T. Nurgaliev, Prof. D.Sc. LatchezarAvramov Institute of Electronics “Acad. Emil Djakov”, Bulgarian Academy of Sciences 72 Tsarigradsko chaussee blvd., 1784 Sofia, Bulgaria Prof. DVM, D.Sc. Hristo Najdenski, Assoc. prof. M.D. PhD Vesslin Kussovski The Stefan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 GeorgiBonchev str., 1113 Sofia, Bulgaria

Resume : Long-term use of ocular prostheses causes frequent ocular infections with a number of adverse effects.The antibacterial and antifungal activity was investigated of Ag-doped Al2O3 nanolayers deposited by radioactive magnetron sputtering on ocular prostheses after a two-year period. A microbiological study was performed to determine the antibacterial action of the nanocomposite Ag/Al2O3 nanolayers against gram positive and gram negative bacteria, and an antifungal action against Candida albicans. From the conducted microbiological studies we have found the preservation of the antibacterial and antifungal action of the Ag/Al2O3 nanocoating of the eye prostheses after two years. The results of microbiological studies show that antibacterial and antifungal activity has the same full inactivation by intensity for the reference period. Experimental studies show a very promising application of such antibacterial and antifungal Ag/Al2O3 nanocoating to reduce ocular infections in the placement of ocular prostheses over a prolonged period of use. Keywords: Ag/Al2O3 nanolayers, antibacterial and antifungal action, ocular prostheses coating, two-year period

Authors : Ahmaduddin Khan, Niroj Kumar Sahu
Affiliations : Centre for Nanotechnology Research

Resume : Nanoparticles mediated drug delivery system has immense therapeutic applications especially in the field of cancer treatment. The chemo-therapeutic efficacy is enhanced when combined with radiation therapy or thermal therapy (Hyperthermia). For thermalchemotherapy, appropriate functionalised magnetic nanoparticles with desired magnetic parameter are pivotal. In this direction, we synthesised a highly aqueous dispersible PEG-dicarboxylic acid functionalised iron oxide (Fe3O4) nanocluster with superior magnetic properties by a simple solvothermal approach. The well distributed nanocluster (size~200nm) are formed by the assembly of smaller nanoparticles. Fe3O4 crystallizes in inverse spinel structure with crystallite size of 16.9 nm and possess mesoporous structure with high specific surface area of 71.3 m²/g. The carboxyl group was activated using hydrazine hydrate to form pH sensitive hydrazone bond which further was utilised to attach with doxorubicin (DOX) through C=O linkage. High drug loading efficacy was achieved due mesoporous nature of the nanoparticles and carboxyl group activation. This system shows higher and sustained release at pH 4.3 in comparison to normal physiological pH of 7.4 because of labile hydrazone bond. The synthesised Fe3O4 nanoclusters are biocompatible upto a concentration of 1mg as observed in vitro in MCF-7 breast cancer cell line. Due to higher saturation magnetisation of the nanocluster, a hyperthermic temperature of ~ 43 ºC was achieved in just 105 s at nanoparticle concentration of 2 mg/ml and at the applied AC magnetic field strength of 35.2 kA/m and frequency of 316 kHz. Hence, the PEGylated mesoporous Fe3O4 nanoclusters can be effectively utilised for thermochemotherapy of cancer.

Authors : Jooran Kim
Affiliations : Korea Institute of Industrial Technology

Resume : 3D bioprinting is a widely used technology to dispense cell-laden biomaterials for rapid fabrication of complex 3D tissue constructs or artificial organs. To date, many studies have been investigated the deposition and patterning of cell-laden bioinks with a 3D bioprinter. However, the precise positioning, reasonable mechanical properties, and controlled cell distributions of constructs in 3D bioprinting system still remain technical challenges. In this study, we developed heterogeneous cell-laden microchannel network using human umbilical vein endothelial cell-cardiomyocytes by a direct patterning with a 3D bioprinter. We fabricated 3D tissue constructs consisting of the core (human umbilical vein endothelial cell -laden collagen) and the sheath (cardiomyocytes-laden gelatin methacrylate). We could achieve a stable 3D multilayered core-sheath structure with the reasonable elastic modulus. This system also facilitated cell alignment and migration within each constructs and promoted vascular network with high cell viability. Calcium imaging was used to optically probe intracellular calcium ion signals during excitation-contraction coupling in cardiomyocytes within 3D vascular network. This paper presents the new approach for fabricating vascularized heterogeneous 3D scaffolds and highly controlled deposition technique of bioinks. The cell-laden 3D constructs could be extended to serve as in vitro models for clinical cardiovascular disease researches and cardiovascular tissue regenerations.

Authors : A.I. Visan1*, I. Jinga1, I. Ungureanu1, F. Antohe2, M.C. Chifiriuc3, R. Cristescu1
Affiliations : 1 Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, Magurele, Ilfov, Romania; 2 Proteomics Department, Institute of Cellular Biology and Pathology “N. Simionescu” Romanian Academy, Bucharest, Romania; 3 Department of Microbiology, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania *Corresponding author:

Resume : Polymer-coated stents with antiproliferative drugs and growth factor have been proposed to defeat adverse reactions which occur in cardiovascular treatment. We report on successful deposition of functionalized thin films of (everolimus and paclitaxel) drugs encapsulated in complex matrices [either blends of poly(L-lactide) and collagen or nanoparticles of poly(lactic-co-glycolic acid) - polyvinyl alcohol] containing vascular endothelial growth factor (VEGF) by Matrix Assisted Pulsed Laser Evaporation (MAPLE). The morphology, hydrophilicity, and biodegradability of the composite coatings have been investigated. The main recipes of the drug functionalized polymer matrices, synthesized by MAPLE, have been validated by biological investigations, drug release profiles, and physical-chemical investigations. In vitro evaluation tests performed on the fabricated thin films have revealed great biocompatibility, that may endorse them as competitive candidates for the development of improved non-toxic surfaces resistant to microbial colonization. The proposed functionalized thin films apart from preventing the restenosis (due to selected drug inhibitors), also could eliminate the risk of late thrombosis (as the covered stent is replaced by connective tissue thanks to VEGF addition) and are expected to act as improved/appropriate/effective coatings for the next-generation drug-eluting stents.

Authors : Anita Visan1, Carmen Ristoscu1, Gianina Popescu-Pelin1, Mihai Soprony1, R. Cristescu1, Carmen Mariana Chifiriuc3, David Grossin4, Fabien Brouillet4, Ion N. Mihailescu1
Affiliations : 1 National Institute for Lasers, Plasma and Radiation Physics, Magurele, Ilfov, Romania ; 2 National Institute of Materials Physics, Magurele, Ilfov, Romania;3Department of Microbiology, Faculty of Biology, University of Bucharest, 060101; Research Institute of the University of Bucharest –ICUB, Spl. Independentei 91-95, Bucharest, Romania;4 CIRIMAT – Carnot Institute, University of Toulouse, ENSIACET; 4 Allée Emile Monso, 31030 Toulouse Cedex 4, France

Resume : We report on the successful deposition of antimicrobial chitosan-biomimetic nanocrystalline apatite –tetracycline thin films by Matrix Assisted Pulsed Laser Evaporation (MAPLE) using a KrF* excimer laser source (λ = 248 nm, ζFWHM ≤ 25 ns). Typical FTIR spectra of the obtained thin films were found to be highly similar to the spectrum of the initial powders. Scanning electron microscopy have evidenced a typical morphology characteristic to the deposition technique, advantageous for medical application, the nanoscale roughness increasing with the chitosan concentration. We have evaluated the antibacterial properties of the thin films containing chitosan and tetracycline deposited by MAPLE on titanium samples, using as model organisms both the Gram-negative E. coli and Gram-positive E. faecalis. The biocompatibility of the obtained films deposited on Ti substrates was evaluated by in vitro tests on human bone osteosarcoma cells. These tests have revealed the morphology and cellular cycle of the cells growing on the obtained thin films. The results have demonstrated that the chitosan- biomimetic nanocrystalline apatite-tetracycline composite thin films have improved the bone formation and further facilitated the anchorage between the bone and prosthesis, thus validating the MAPLE efficiency.

Authors : Iole Venditti 1, Chiara Battocchio 1, Luca Tortora 1, Giovanna Iucci 1, Martina Marsotto 1, Marina Porchia 2, Francesco Tisato 2, Maura Pellei 3, Carlo Santini 3 Corresponding Author, e-mail:
Affiliations : 1 Sciences Dept. Roma Tre University of Rome, Italy 2 ICMATE, National Research Council (CNR), Padua, Italy 3 School of Science and Technology, Chemistry Division, University of Camerino, Italy

Resume : Gold nanorods (AuNRs) are successfully employed in drug delivery, biosensors, and biotechnologies [1,2]. Their wide success is due to their unique chemical properties, biocompatibility, easy, cheap and versatile synthesis. In this framework, hydropilic AuNRs were synthetized with the aim to obtain strongly hydrophilic nanomaterials, suitable for drug delivery. AuNRs were investigated by UV-Vis-NIR, FT-IR and HR-XPS spectrocopies, confirming nanosize, with typical surface plasmon resonance (SPR) bands at 550 nm and 900 nm, and surface functionalization by ascorbic acid (AA) and cetyl trimethyl ammonium bromide (CTAB). Therefore, AuNRs were used as drug delivery system for copper (I)-based anti-tumor complex namely [Cu(PTA)4]+[BF4]- (PTA = 1,3,5-triaza-7-phosphadamantane) [3-5]. The loading procedures and efficiency of Cu(I) complexes on the AuNRs surface were optimized in order to control their bioavailability and release. References [1] Park K., Drummy L. F., Wadams R. C., Koerner H., Nepal D., Fabris L., Vaia R. A. Growth Mechanism of Gold Nanorods. Chem. Mater. 2013; 25: 555-563 [2] Venditti I. Engineered gold-based nanomaterials: morphologies and functionalities in biomedical applications. A mini review. Bioengineering. 2019; 6(2): 53 [3] Gandin V., Tisato F., Dolmella A., Pellei M., Santini C., Giorgetti M., Marzano C., Porchia M. In Vitro and in Vivo Anticancer Activity of Copper(I) Complexes with Homoscorpionate Tridentate Tris(pyrazolyl)borate and Auxiliary Monodentate Phosphine Ligands. J. Med. Chem. 2014, 57 (11): 4745-4760. [4] M. Porchia, F. Benetollo, F. Refosco, F. Tisato, C. Marzano, V. Gandin. Synthesis and structural characterization of copper(I) complexes bearing N-methyl-1,3,5-triaza-7-phosphaadamantane (mPTA): Cytotoxic activity evaluation of a series of water soluble Cu(I) derivatives containing PTA, PTAH and mPTA ligands. J. Inorg. Biochem. 2009; 103 (12): 1644 [5] Fratoddi I., Venditti I., Battocchio C., Carlini L., Porchia M., Tisato F., Bondino F., Magnano E., Pellei M., Santini C. Highly hydrophilic gold nanoparticles as carrier for anticancer copper(I) complexes: loading and release studies for biomedical applications. Nanomaterials. 2019; 9: 772 Acknowledgements: The Grant of Excellence Departments, MIUR (ARTICOLO 1, COMMI 314 – 337 LEGGE 232/2016), is gratefully acknowledged by authors of Roma Tre University.

Authors : Iole Venditti1, Irene Schiesaro1, Martina Marsotto1, Maura Pellei2, Carlo Santini2, Luca Bagnarelli2, Giovanna Iucci1, Carlo Meneghini1, Chiara Battocchio1
Affiliations : 1 Dept. of Science, University of Roma Tre, Viale G. Marconi 446, Rome, Italy. 2 School of Science and Technology, Chemistry Division, University of Camerino, Camerino (MC), Italy.

Resume : Copper complexes are coming out as metal-based drugs candidates for the treatment of cancer, due to their wide structural variability, biologically accessible redox properties and bioavailability. Recently, in our quest to find suitable ligands in the design of copper-based anticancer agents, we focused our attention on the synthesis of copper complexes of bis(azol-1-yl)carboxylate ligands functionalized with biomolecules. In addition, Cu(II) complexes of alkyl bis(pyrazol-1-yl)acetate ligands have been investigated for the development of a new and more efficient promoter for the Kharasch-Sosnovsky reaction to oxidize alkenes in allyl position. Since such coordination compounds have low solubility in aqueous medium, it is necessary to design a strategic approach allowing for drug delivery. By conjugating the copper complexes with hydrophilic gold nanoparticles, it is possible to improve their solubility and stability in water, increasing their bioavailability. Moreover, these drug delivery systems allow the investigation of a slow and controlled release of copper complexes [1]. In this context, we carried out a spectroscopic investigation of the molecular, electronic structure and coordination geometry of a selection of Cu(II)-coordination compounds, by means of complementary X-ray techniques induced by Synchrotron Radiation: the molecular and electronic structure were probed by means of SR-XPS and NEXAFS, the oxidation state and the local coordination chemistry of the metal ion were assessed by Cu K-edge XAFS analysed in the near edge (XANES) and extended (EXAFS) regions.

Authors : Su-Geun Yang
Affiliations : Department of Biomedical Science, College of Medicine, Inha University

Resume : In this study, poly-L-dopa nanoparticle-based drug delivery system was designed for near-infrared light controlled trimodal therapy in which photodynamic therapy (PDT), photothermal therapy (PTT) and chemotherapy are combined to produce synergistic therapeutic effects. Poly-L-dopa nanoparticles (PLD NPs) were synthesized by oxidation and self-polymerization of L-DOPA with KMnO_4. For designing purposes of PTT, PLD NPs were modified with the photosensitizer, pheophorbide a (PheoA), via reducible disulfide linker for glutathione-responsive intracellular release. Due to the ability to convert NIR light into heat, PLD NPs could be used as a photothermal agent without further modification for PTT. Also, PLD NPs have a fluorescence resonance energy transfer- (FRET) based self-quenching effect which reduces the side effects by keeping the system photo-inactive during blood circulation. PheoA modified PLD NPs (PLD-PheoA NPs) were loaded with chemotherapy drug, doxorubicin (Dox), through ᴨ-ᴨ stacking and hydrogen bonding. The in vitro antitumor effect of PLD-PheoA/Dox NPs with trimodal PDT/PTT/chemotherapy was investigated in terms of photothermal efficiency, photoactivity, stimuli-responsive drug release, cellular uptake, and therapeutic efficacy. The best of our knowledge, this is the only study presented in literature, using poly-L-dopa nanoparticles with trimodal synergistic therapy approach for cancer treatment.

Authors : Ji-Eun Lee, Seung-Min Lee, and Kwang-Ho Lee
Affiliations : Advanced Materials Science and Engineering, College of Engineering, Kangwon National University, Republic of Korea

Resume : To make micropattern including bio-substances on nano-mesh, we developed the photo-crosslinking process using hydrogel. For in-situ micropatterning, the synthesized hyaluronic acid was empolyed with transparent micromold and UV irradiation. The photosensitive hyaluronic acid was rapidly cross-linked through the porous nano-mesh. When hyaluronic acid is patterned on the nanomesh, drugs and cells can be stably selected, and the release of drugs and the behavior of various cells can be controlled according to the patterning conditions. The immobilized material was released by diffusion, which was very precisely controlled through the coating of hyaluronic acid and nanomesh, and the theoretical verification with finite element analysis was performed. The proposed research could be used in the field of tissue engineering for local drug delivery or regeneration of damaged tissues in the body.

Start atSubject View AllNum.Add
08:45 Plenary Session - (08. 45 – 09.45): Lecture by Prof. André Geim Nobel Laureate in Physics (2010) University of Manchester, U.K.    
09:45 The Nobel Prize in Physics 2010 was awarded jointly to Andre Geim and Konstantin Novoselov “for ground breaking experiments regarding the two-dimensional material graphene”    
09:45 10.00 - Coffee Break    
NEW FRONTIERS IN SMART CARBON. Invited Presenters Session : Invited Chairs - Professors Peter Scharff (Germany), Maurizio Prato (Italy), Hsing-An Lin (China) and Jun Maruyama (Japan)
Authors : Maurizio Prato
Affiliations : Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste, Italy CICbiomaGUNE, San Sebastián, Spain

Resume : Connecting nanostructured materials to biological compartments is a crucial step in prosthetic applications, where the interfacing surfaces should provide minimal undesired perturbation to the target tissue. Ultimately, the (nano)material of choice has to be biocompatible and promote cellular growth and adhesion with minimal cytotoxicity or dis-regulation of, for example, cellular activity and proliferation. In this context, carbon nanomaterials, including nanotubes and graphene, are particularly well suited for the design and construction of functional interfaces. This is mainly due to the extraordinary properties of these novel materials, which combine mechanical strength, thermal and electrical conductivity. Our group has been involved in the organic functionalization of various types of nanocarbons, including carbon nanotubes and graphene. The organic functionalization offers the great advantage of producing soluble and easy-to-handle materials. As a consequence, since biocompatibility is expected to improve upon functionalization, many modified carbon nanomaterials may be useful in the field of nanomedicine. In particular, we have shown that carbon nanotubes can act as active substrates for neuronal growth, a field that has given so far very exciting results. Nanotubes are compatible with neurons, but, especially, they play a very interesting role in interneuronal communication. Improved synaptic communication is just one example. Research toward nerve regeneration in damaged tissues has also given very interesting results. During this talk, we will show the latest and most exciting results obtained in our laboratories in these fast developing fields.

Authors : Ji-Young Hwang, Seung Beom Kang, Sang Won Lee, Ueon Sang Shin, Sang-Hoon Lee
Affiliations : Research Center for Carbon Convergence Materials, Korea Institute of Carbon Convergence Technology (KCTECH), Jeonju, Republic of Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea; Department of Biomedical Engineering, Korea University, Seoul, Republic of Korea

Resume : Engineering of the surface is the multidisciplinary research of materials science and has a broad range of applications to chemistry, biology, engineering and medicine. Surface modification of a biomaterial can be done by different methods for altering into required characteristics, such as size, morphology, topology, wettability, roughness, surface charge, reactivity, biocompatibility and applicability. Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions of tissues. Here we provide an overview of the various applications of our surface modification systems and also discuss successes to date, current limitations and future directions. We developed surface modification system of nanotubes, nanoparticles, polymers, plastic wares, polydimethylsiloxane chips, using chemicals, zwitterionic polymers, polyethylene glycol (PEG), 3-aminopropyltriethoxysilane (APTES), extracellular matrix (ECM), other bioactive molecules, etc for efficiently enhancing cell functions. Our results highlight an innovative surface-modified biomaterials for advance cell culture and tissue regeneration system and may allow the development of enabling technologies for tissue engineering and regenerative medicine applications.

Authors : Hsing-An Lin1,2,3, Yoshikatsu Sato4, Yasutomo Segawa2,3, Taishi Nishihara2,3, Kakishi Uno3, Lawrence T. Scott5, Tetsuya Higashiyama3,4, Kenichiro Itami2,3,4
Affiliations : 1 School of Materials Science and Engineering, Shanghai University, Shanghai, 200444, China. 2 JST ERATO, Itami Molecular Nanocarbon Project, Chikusa, Nagoya 464-8602, Japan. 3 Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan. 4 Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Japan. 5 Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467-3860, USA

Resume : Incorporating hydrophilic tetraethylene glycol with saddle-shaped nanographene make water-soluble warped nanographene (WNG) has good solubility in organic solvents and water. It exhibits strong greenyellow fluorescence, high photostability, and low cytotoxicity. The aforementioned properties imply that water-soluble WNG is applicable to live-cell imaging. Subsequently, water-soluble WNG was applied for HeLa cell imaging. The results indicated that water-soluble WNG internalized in HeLa cells and accumulated into the lysosome as aggregates. During live-cell imaging by water-soluble WNG, we serendipitously discovered that the HeLa cells stained with water-soluble WNG could be killed by selective laser irradiation. In the presence of 5.0 mM of water-soluble WNG, the light irradiated HeLa cell shrunk, generated blebs, and finally died. Moreover, dose-depended cell viability is observed. The cell viability was 98% in the presence of 0.01 mM of water-soluble WNG. Upon increasing the concentration of water-soluble WNG from 0.01 to 1.0 mM, a dramatic decrease in cell viability was observed, and 91% of the cells were killed in the presence of 1.0 mM water-soluble WNG under irradiation. Using 5.0 mM water-soluble WNG, almost no cells survived under the irradiation. In contrast, even in the presence of water-soluble WNG, no obvious change in cell morphology and viability was observed without light stimulation. Although the mechanism is unclear, the relatively high efficiency of the singlet oxygen generation of water-soluble WNG may contribute to its HeLa cell death. [1] Hsing-An Lin, Yoshikatsu Sato, Yasutomo Segawa, Taishi Nishihara, Kakishi Uno, Lawrence T. Scott, Tetsuya Higashiyama, Kenichiro Itami, Synthesis of a water-soluble warped nanographene and its application for photo-induced cell death. Angew. Chem. Int. Ed. 2018, 57, 2874-2878. (VIP)

Authors : Jun Maruyama, Tsutomu Shinagawa, Mitsuru Watanabe, Yukiyasu Kashiwagi, Shohei Maruyama, Toru Nagaoka, Wakana Matsuda, Yusuke Tsutsui, Shu Seki, Hiroshi Uyama
Affiliations : Osaka Research Institute of Industrial Science and Technology; Kyoto University; Osaka University

Resume : Cylindrical self-assembly of uniform polystyrene nanoparticles with fructose was carried out by hydrothermal treatment in the presence of both carbon nanofiber and sodium alginate, which is followed by heat treatment in an inert atmosphere. The carbonization generated fructose-derived honeycomb-like carbon walls with helically-aligned nanopores left after the polystyrene decomposition. The diffuse reflectance circular dichroism measurements gave peaks with opposite signs for the D- and L-fructose-derived cylindrical carbons. Circularly polarized light sensitivity in transient photoconductivity was confirmed apparently in the carbon-based helical structures. This sensitivity as well as straightforward formation of composites with another component to give helicity showed potential applications of the helically-aligned pores.

Authors : Rachel Yerushalmi - Rozen, Evgenee Yekymov, Eugene Katz,
Affiliations : Dept. of Chemical Engineering Ben-Gurion University of the Negev Israel; Dept. of Solar Energy and Env. Phys. the, Jacob Blaustein Institute for Desert Research, BGU

Resume : Exohedral van der Waals (vdW) hybrids comprising carbon nanotubes (CNT) and fullerene molecules (C60) may be used for engineering of carbon nano-materials with tunable physiochemical properties. As the hybrids preserve the sp2 hybridization of the components, superior electron transport, low percolation threshold, good electron accepting and efficient singlet oxygen sensitizing ability are expected. These hybrids may be useful as electron acceptors and charge carriers in bulk-heterojunction organic photovoltaics (OPV). In the reported study we demonstrate that induced SP3 point defects in CNT (via UV/O3 treatment) can be used for controlling the nano-structure of CNT- C60 hybrids. We describe a two-step process for assembly of CNTs networks and thermal (vacuum) sublimation of fullerenes nanocrystals. Transmission electron microscopy indicates that the hybrids comprise of random 3D scaffolds of individual CNT and fullerite nanocrystals, and Raman analysis reveals their non-covalent nature. While surface migration of the fullerenes on the CNT surface leads to coarsening of the nanocrystals, we find that controlled induction of SP3 point defects in the CNT can trap the initial nano-morphology and preserve the nano-dimensions and structure of the fullerene crystals, also at elevated temperatures and prolonged annealing. The nano-morphology of the treated nanotubes is preserved also when the hybrids are coated by a polymer layer (poly(3-hexylthiophene-2,5-diyl, P3HT), and the polymer-hybrid films show significant quenching of the photoluminescence, indicating that these hybrids could be useful in photovoltaic applications.

Authors : Prabhat Kumar, Martin Šilhavík, Zahid Ali Zafar, and Jiří Červenka
Affiliations : Department of Thin Films and Nanostructures, FZU - Institute of Physics of the Czech Academy of Sciences, Prague 16200, Czech Republic

Resume : We demonstrate an ultrasensitive strain-gauge sensor using a conductive and flexible 3D graphene aerogel that enables detecting small pressures and various biological signals from the movement of human skin. The active element of the sensor is composed of a 3D graphene nanoporous framework made of graphene sheets separated with air-filled pores. The 3D graphene aerogel is synthesized using a simple hydrothermal technique followed by freeze-drying and thermal annealing. The resulting graphene aerogel is elastic and exhibits a significant change of electrical resistance with strain. It is found that the resistance of the sample is highly sensitive to applied strain, enabling the detection of small changes of the sample size down to a few micrometers. The responsivity of the samples allows detecting fast events down to a few microseconds. We show that the 3D graphene-based strain-gauge can be used as a wearable electronic sensor for human heartbeat monitoring as well as for other kinds of biological motion detection.

Authors : Cathal Larrigy, Eoghan Vaughan, Nadim Shahin, Alessandra Imbrogno, Pingyang Ma, Daniela Iacopino, Micheal Burke, and Aidan J. Quinn
Affiliations : Tyndall National Institute, University College Cork,

Resume : There is increasing interest in laser methods for direct-write formation of graphitic and graphene-like carbon structures from polymers, especially under ambient conditions. Applications include direct incorporation of sensing functionality (e.g., electrochemical, temperature, humidity, strain) onto plastic components. While the most popular material for laser-induced graphitization is polyimide, laser-induced graphitization of renewable materials through “multiple lasing” processes has been explored recently. Chitosan, the second most abundant natural polysaccharide after cellulose, is derived from partial deacetylation of chitin (found in insect exoskeletons, some crustaceans and fungi cell walls). Here we report on site-selective laser-induced formation of porous 3D graphene on flexible and water-soluble chitosan films using low-cost lasers. Raman spectroscopy demonstrates formation of high-quality graphene through the presence of sharp 2D peak. Measured full-width at half maximum intensity values (FWHM) values for the 2D peak were FWHM(2D) < 100 cm-1 (wavenumbers). We have also demonstrated proof-of-principle electrochemical sensing of with two redox systems: outer-sphere [Ru(NH3)6]3+/2+ and inner-sphere [Fe(CN)6]3-/4-. Quasi-reversible Nernstian behavior was observed in cyclic voltammetry measurements for both systems over time scales ~ 20 minutes. Measurements at longer time-scales showed evidence of electrode degradation, consistent with dissolution of the biopolymer substrate. These results showcase the potential of these exciting materials for development of compostable sensors which could be used in sustainable packages for perishable food, e.g., dairy or meat products.

13:00 14:00 Lunch Break    
Authors : Mandeep Singh, Dr. Ashish Gupta, Dr. S. R Dhakate, Dr. Shashank Sundriyal
Affiliations : 1. CSIR-National Physical Laboratory, New Delhi, India 2. Academy of scientific and innovative research, NPL campus, New Delhi

Resume : PAN (Polyacrylonitrile), Rayon and Pitch are three main precursors for carbon fibers where PAN is used mostly due to its good thermal stability, high carbon content and good tensile strength. However, PAN-based carbon nanofibers limit its use in bulk due to the high cost of precursor i.e. PAN. So there is a need to develop carbon fibers from low-cost precursors. Biopolymers such as lignin and cellulose have high carbon content and have the potential to become a low-cost precursor due to their abundance in nature. Lignin is the second most abundant biopolymer after cellulose. Due to the highly complex structure of lignin, it is very difficult to spun it alone in the form of fibers. So, we have blended lignin with PVA (polyvinyl alcohol) and spun the blend using electrospinning. In this work, we present the optimization and synthesis of carbon nanofibers from Lignin: PVA blend using the electrospinning technique. Further, these mats were characterized by their electrochemical properties. SEM analysis shows that lignin: PVA based carbon nanofibers have a diameter in range 600-850 nm. These nanofiber mats were further characterized for their microstructural, thermal and electrical properties using XRD, RAMAN, TGA, DSC, BET. These mats show a specific capacitance of 145F/g at 1A/g.

Authors : Yu Zhao, Yi Tao, Kabin Lin, Jingjie Sha and Yunfei Chen
Affiliations : School of Mechanical Engineering, Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China

Resume : In this work, in order to elucidate the intrinsic mechanism of thermal transport along different crystallographic planes in layered materials, we propose a simple and convenient method to manufacture inclined graphite applying Focused Ion beam (FIB). Then, the thermal conductivities along several crystallographic planes in graphite at room temperature are measured using the time-domain thermoreflectance (TDTR) method. To address the issue of lacking in-plane symmetry in inclined graphite, a three dimensional thermal model is developed to extract the thermal conductivity for layered materials from the measured signals of the TDTR method. In addition, for comparison with the cut graphite samples and extracting the in-plane and cross-plane thermal conductivities of graphite sample, a piece of graphite flake is prepared to conduct the TDTR experiment. Our experimental results show that the processing method is reliable and validate the three dimensional thermal model which we develop is appropriate to describe the thermal transport in layered materials. More important is that the results also provide a direct experimental evidence for the classical anisotropy model derived from the Fourier’s law can well predicting the thermal transport along an arbitrary crystallographic plane in layered materials. Furthermore, for graphite, after phonons undergoing the sufficient scattering processes, the in-plane phonon modes dominate the process of thermal transport along all crystallographic planes except the cross-plane direction owing to the value of the in-plane thermal conductivity is much larger than that of the cross-plane conductivity. Our study provides a new perspective on the thermal transport in graphite and other layered materials, and provides an important design guideline for actively manipulating the direction of thermal transport in nano-layered materials.

15:30 16:00 Coffee Break    
Round Table Discussion : invited 7en minutes Key Speech on Frontier Research
Authors : Oleksandr Ivanyuta1, Eugenia Buzaneva2, Uwe Ritter3, Peter Scharff3
Affiliations : 1 Faculty of Radiophysics Electronics and Computer Systems, Taras Shevchenko National University of Kyiv, Ukraine E-mail: 2 The Scientific and Training Center on Physical and Chemical Material Science, National Taras Shevchenko National University of Kyiv and NAS of Ukraine 3 Institute of Chemistry & Biotechnology, T U Ilmenau, Germany

Resume : Biofunctionalization of carbon nanotubes, which are well-known for their unique complex of properties, is in the centre of research attention for numerous biomedical applications, also as for fundamental study of nanotubes as templates. Covalent or non-covalent immobilization of biomolecules on carbon nanotubes opens the doors for novel biocomposite manufacturing and biosensing. Carbon nanotube functionalization by DNA, RNA, oligonucleotides or single nucleotides is of special interest as possible approach for nucleic acid sensing, gene delivery, gene therapy, clinical diagnosis and pathogen monitoring. It is also used in nanotechology as technique for suspensioning, characterization and separation of nanotubes. In our investigations the non-covalent immobilization in aqueous suspensions of AMP and DNA on multi-walled carbon nanotubes developed. Previous oxidation of multi-walled carbon nanotubes by ozonolysis were used if it was necessary. The characterization of functionalized nanotubes was carried out by Scanning Electron Microscopy, Transmission Electron Microscopy, Optical Microscopy, Raman, UV-visible-NIR, IR- and Photoluminescence Spectroscopies. Various models of interface formation between biomolecules and nanotube surface are discussed. Optical characteristics of novel biofunctionalized nanotubes also gave us necessary information for their future application as biosensors or activating agents.

Authors : Jun Maruyama, Tsutomu Shinagawa, Mitsuru Watanabe, Yukiyasu Kashiwagi, Shohei Maruyama, Toru Nagaoka, Wakana Matsuda, Yusuke Tsutsui, Shu Seki, Hiroshi Uyama
Affiliations : Osaka Research Institute of Industrial Science and Technology; Kyoto University; Osaka University

Resume : Potential applications of cylindrical carbon with helically aligned pores were explored based on its concomitant properties. The first example is transient photoconductivity (ϕΣμ), in which ϕ is the photocarrier generation yield and Σμ is the sum of the charge-carrier mobilities. The flash-photolysis time-resolved microwave conductivity (FP-TRMC) technique was conducted using circularly polarized light excitation at 532 nm. The ϕΣμ difference at the cylindrical carbon synthesized using D-fructose was observed for the left- and right-handed circularly polarized light. In contrast, no appreciable ϕΣμ difference was observed for the cylindrical carbon synthesized using the racemic fructose. These results indicated the potential to form an optoelectronic device that could change the conductivity by the circularly-polarized light. The second example is the feasibility to form composites with the helical structure due to easy access of the constituents to the pores. A composite of the cylindrical carbon and colloidal Au nanoparticles (Au NPs) was formed by immersing the cylindrical carbon in the colloidal Au dispersion in toluene and rinsing with the dispersion medium. The Au NPs clusters were helically aligned like stepping stone. This simple formation method enabling the helical alignment of the Au NP clusters implied the potential of the cylindrical carbon as a platform for feasible generation of helical structures.

Authors : Hsing-An Lin1,2,3, Yoshikatsu Sato4, Yasutomo Segawa2,3, Taishi Nishihara2, Kakishi Uno3, Lawrence T. Scott5, Tetsuya Higashiyama3,4, Kenichiro Itami2,3,4
Affiliations : 1. Department of Polymer Materials, Shanghai University, Baoshan, Shanghai, 200444, China.; 2. JST ERATO, Itami Molecular Nanocarbon Project, Chikusa, Nagoya 464-8602, Japan.; 3. Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan.; 4. Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Japan.; 5. Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467-3860, USA

Resume : Nanographene, a small piece of graphene, has attracted unprecedented interest across diverse scientific disciplines particularly in organic electronics.1 The biological applications of nanographenes such as bioimaging, cancer therapies, and drug delivery would provide significant opportunity and breakthrough in the field. However, intrinsic aggregation behavior and low solubility of nanographenes stemming from the flat structures hamper their development in bioapplications. Herein, we report a water-soluble warped nanographene (WNG)2 that can be easily synthesized by a sequence of regioselective C–H borylation and cross-coupling of saddle-shaped WNG core structure. The high water solubility of water-soluble WNG is ensured by its saddle-shaped structure with hydrophilic tetraethylene glycol chains. The water-soluble WNG possesses a range of additional promising properties such as long lifetime fluorescence, good photostability and low cytotoxicity. Moreover, the water-soluble WNG has been successfully applied for live HeLa cell imaging and photo-induced cell death.

18:30 Graduate Student Awards Ceremony    
19:55 SOCIAL EVENT    
Start atSubject View AllNum.Add
08:45 Plenary Session - (08. 45 – 09.45): Lecture by Prof. Ulrike Diebold TU Vienna, Austria    
09:45 Coffee Break    
13:00 Lunch Break    
Authors : Chang Kyu Jeong
Affiliations : Division of Advanced Materials Engineering, Jeonbuk National University, Jeonju, Jeonbuk 54896, Republic of Korea

Resume : Elastic composite-based piezoelectric energy harvesting technology is highly desired to enable a wide range of device applications, including self-powered wearable electronics, robotic skins, and biomedical devices. Recently developed piezoelectric composites are based on inorganic piezoelectric fillers and polymeric soft matrix to take the advantages of both components. However, there are still limitations such as the weak stress transfer to piezoelectric elements and the poor dispersion of fillers in matrix. In this talk, a highly-enhanced piezocomposite energy harvesters (PCEH) is representatively developed using a three-dimensional (3D) interconnected electroceramic skeleton by mimicking and reproducing the sea porifera architecture (although the speaker will introduce other research examples and achievements). This new mechanically reinforced PCEH is demonstrated to resolve the problems of previous reported conventional piezocomposites, and in turn induces stronger piezoelectric energy harvesting responses. The generated voltage, current density and instantaneous power density of the biomimetic PCEH device reach up to ~16 times higher power output than that of conventional randomly-dispersed particle-based PCEH. This work broadens the further developments of high-output elastic piezocomposite energy harvesting and sensor application with biomimetic architecture.

15:30 Coffee Break    
Novel applications of nanoparticles and nanostructures in biological systems. Invited Presenters : Session Organizer - Professor Peilin Chen (Academia Sinica, Taiwan)
Authors : Dar-Bin Shieh, DDS, DMSc
Affiliations : 1 Institute of Oral Medicine and Department of Stomatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan 2 International Institute of Macromolecular Analysis and Nanomedicine Innovations (iMANI), Center of Applied Nanomedicine, Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan, Taiwan.

Resume : We developed a type of zero-valent iron nanoparticle (ZVI NP) that exhibits selective anti-cancer potency through non-apoptotic cell death in several cancer types. In human head-and-neck cancers (HNC), we demonstrated that ZVI NPs selectively induce cancer ferroptosis yet spare the normal cells and presented strong efficacy in human cancer bearing rodent model without detectable systemic toxicity. Mitochondria and lysosome dependent pathways play critical roles in the ZVI NPs induced cancer cells death. Further investigation revealed that 10 ferroptosis associated gene panel may serve to predict their susceptibility to ZVI NPs treatment. Combination of ferroptosis inducers with ZVI could reverse ZVI therapeutic resistance. In addition, we also discovered that ZVI NPs inhibited the allograft growth of mouse Lewis lung carcinoma. Strikingly, multi-color fluorescent-immunohistochemistry demonstrated that the infiltrated M1 macrophages increased in tumor site whereas M2 macrophage located toward the tumor periphery after ZVI NPs treatment. ZVI also significantly down regulate PD-L1 in tumor infiltrating T cells. These results suggested that ZVI NPs inhibit tumor growth in vivo through both direct induction of cancer cell ferroptosis and modulation of tumor microenvironmental immune system towards anti-cancer phenotype.

Authors : Yu-Hsuan Chen, Hsuen-Li Chen, Dehui Wan
Affiliations : Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan (Yu-Hsuan Chen, Dehui Wan); Department of Material Science and Engineering, National Taiwan University, Taipei, Taiwan (Hsuen-Li Chen)

Resume : Nowadays, problems of energy crises and climate change pose a threat to human living. Therefore, developing eco-friendly cooling systems is an utmost issue to work on. Most of current cooling methods require energy to carry heat away. By means of passive radiative cooling, the system can radiate heat to outer space through the atmospheric transmission window between 8-13 ?m, thus lowering the temperature without any energy consumption. Some photonic solar reflector and thermal emitter, like HfO2, SiO2 and metamaterial film, can reflect incident sunlight while emitting radiation in the atmosphere windows. However, with its complicated fabrication and high cost, nanophotonic approach is hard to scale up to meet the requirements of commercial applications. Meanwhile, we focus on natural materials of great potential which are still not well-studied. Compared to the typically narrow Infrared (IR) emissivity peaks of artificial daytime cooling materials, the extremely broadband emissivity peak of the natural materials could cover two atmospheric windows (8-13 ?m and 16-25 ?m). Here, we present a study on silk cocoon, which is characterized by its 90% absorption at IR wavelengths and simple, well-studied fabrication process for different morphologies. We fabricate silk fibroin thin film with thickness ranging from 6 to 88 ?m by adjusting the concentration of silk fibroin solution. We observe the broadening of thickness leads to an increase in emissivity over IR wavelength regions, indicating thickness is the key factor to optical and radiative control. In addition to simulated spectra, optical constants of silk film are considered for further analysis. We also investigate the board band absorption and extinction coefficient at NIR wavelength. Throughout the IR wavelength, silk film exhibits a maximum absorbance of 92% at the thickness of 100 ?m. However, absorbance of silk film has positive correlation with thickness in solar spectrum region, resulting in high Psun performance. Therefore, thermal simulation is applied to find the lowest Tequ, at which the optimal thickness of silk film is located. Finally, we conduct temperature measurement on silk film practically to verify its daytime radiation cooling ability. With silk covering, the temperature of mobile phone decrease by 3.5 K, while the average absorbance of mobile phone is significantly enhanced to 94 % in atmospheric window.

Authors : Tzu-Ting Tseng,?, Yi-Ping Chen, *,?,?, Yun-Pu Chang,? Chung-Yuan Mou,?,? and Si-HanWu*,?,?
Affiliations : ?Department of Chemistry, National Taiwan University, Taipei 106, Taiwan ?Department of Chemistry, University of California Davis, California 95616, United States ?Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 110, Taiwan ?International Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan

Resume : Mesoporous silica nanoparticles (MSNs) is a promising nanocarrier for delivering anti-tumor drugs to cancer. Polyethylene glycol (PEG) has been linked to many nanocarriers to increase the dispersity, bioavailability, and circulation time of nanoparticles. However, on the other hand, PEGylation could limit the cellular uptake and endosomal escape of MSN, resulted in significant loss of activity of the delivery system. In this study, to overcome the ?PEG dilemma?, we synthesized pHR-MSN-PEG/DA@EPIs, in which pH-responsive (pHR) core-shell MSNs were utilized as nanocarriers, the anti-tumor drug epirubicin (EPI) was encapsulated by electrostatic interaction, and the amine-containing (DA) functional groups were introduced to control the loading and release of EPI. In vitro studies showed that the plain pHR-MSN-PEG/DA is non-toxic and the cleavage of PEG from carriers was achieved in response to an acidic environment, result in high efficiency of cellular uptake. In addition, pHR-MSN-PEG/DA@EPIs showed considerable cytotoxicity towards 4T1 tumor cells. For in vivo studies, pHR-MSN-PEG/DA showed excellent passive targeting behavior due to the enhanced permeability and retention (EPR) effect, and strong tumor inhibition effects in a chicken embryo chorioallantoic membrane (CAM) tumor model.

Authors : Si-Han Wu, Chung-Yuan Mou, and Yi-Ping Chen
Affiliations : Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 110, Taiwan

Resume : Overcoming the immunosuppressive tumor microenvironment is critical to realizing the potential of cancer immunotherapy strategies. Recent evidences are emerging to show that the cyclic di-guanylate (c-di-GMP), a molecular adjuvant, could induce the production of type I interferons (IFNs) via the stimulator of interferon genes (STING) dependent pathway in antigen presenting cells (APC), leading to enhance the tumor immunogenicity. However, the efficacy of negatively charged c-di-GMP may be limited by some inherent shortcomings, including the poorly membrane permeable, rapid clearance and the inefficiency of cytosolic delivery. Hence, we attempt to develop an alternative ?in situ vaccination? approach based on nanotechnology to initiate an antitumor immune response. In this study, PEGylated RITC fluorescent mesoporous silica nanoparticles (MSN) with a positively charged molecule (N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride, TA) by co-condensation was synthesized to form RMSN-PEG/TA. The characteristics of nanoparticles were determined by transmission electron microscopy (TEM), dynamic light scattering (DLS) and nitrogen adsorption-desorption isotherm. The anionic c-di-GMP was loaded into cationic RMSN-PEG/TA via electrostatic interactions (c-di-GMP@RMSN-PEG/TA), showing the loading amount of c-di-GMP on c-di-GMP@ RMSN-PEG/TA was around 3 wt%. RAW264.7 cells treated with c-di-GMP@ RMSN-PEG/TA obvious increased the production of IL-6, IL-1?, and IFN-? analyzed by real-time PCR, as well as the expression level of phospho-STING (Ser365) protein investigated by western blot. The mouse 4T1 breast tumor-bearing Balb/c mice received injection of c-di-GMP@ RMSN-PEG/TA revealed dramatically tumor growth inhibition accompanied by the infiltration of activated CD11c+ dendritic cells and CD4+ T cells at the tumor site detected by flow cytometry. We validate this in situ vaccination by STING pathway activation provides an attractive therapeutics for breast cancer, highlighting its potential to improve clinical outcomes of cancer immunotherapy.

Authors : Tai-Wei Feng, Jui-Ting Hsiao and Ming-Fa Hsieh
Affiliations : Department of Biomedical Engineering, Chung Yuan Christian University, Taiwan

Resume : Introduction The most common cancer in women is breast cancer and it continues to affect millions of people worldwide. Survival rate increases with early detection but diagnostic procedures may also put patients at risk because of radiation exposure. Development of alternatives such as near-infrared diagnostics may be the key to this problem. Fluorescent dyes may be used but due to their intrinsic characteristics, modification or encapsulation must be done to make it biocompatible. With this, biopolymers may be employed as carriers or encapsulating agents. Experiment Synthesis of polyester-based amphiphilic block copolymers was carried out by ring-opening polymerization reaction. The critical micelle concentration of the copolymers were measured followed by the encapsulation of 2QMEH, a two-photon fluorescent dye. The optimal copolymer for 2QMEH encapsulation showing a loading efficiency of 68.6% was selected. Results and Discussion Encapsulation of 2QMEH gave a two-photon absorption (TPA) cross section of around ~55-120 GM at 820 nm. This is significantly greater than the threshold value for biological applications showing a good potential for in vivo bio-imaging to be used together with near-infrared for breast cancer diagnosis. Acknowledgements: The authors would like to thank the Ministry of Science and Technology of Taiwan for the financial support under grant number MOST 108-2119-M-033-002. References 1) M.L. Gou, X.L. Xheng, K. Men, J. Zhang, L. Zheng, X.H. Wang, F. Luo, Y.L. Zhao, X. Zhao, Y.Q. Wei, Z.Y. Qian, J. Phys. Chem. B., 113, 39 (2009). 2) M. Drobizhev, S. Tillo, N.S. Makarov, T.E. Hughes, A. Rebane, J. Phys. Chem. B., 113, 4 (2009).

Authors : Jau-Ye Shiu, Zhe Lin, Lina Aires, and Viola Vogel
Affiliations : ETH Zurich, department of health science and technology

Resume : Our ability to control cell behavior by properly engineered materials and microenvironments is tightly coupled to understanding the mechanisms of cell-matrix interactions. Anisotropy of extracellular matrix (ECM) drives cell alignment and directional migration during processes like development and wound healing, but also in cancer cell migration and invasion. So far, migrational persistence whether on flat isotropic or anisotropic surface patterns and fibers was mostly studied as local phenomenon asking how specific integrins are responsible for the recognition of the spatial ECM cues. Yet, cell alignment and directional migration in response to external ECM cues requires signal integration across length scales. By producing patterned 2 µm ECM stripes, which lead to cell alignment, and testing the previously described pan-integrin null fibroblast cells, we indeed observed that cell alignment and directional migration on patterned stripes were lost when ?1 integrin was absent. By combining nanopillar arrays with printed cell-adhesive fibronectin (FN) stripes, we could probe subcellular force distributions at submicron resolution. While it was previously recognized that the ?v- and ?1-class integrin signaling pathways are coupled, via myosin II contractility, we discover here that myosin III coupling of these integrin signaling pathways requires a stiff cell nucleus. Importantly, directional migration along adhesive patterned stripes was also impaired for lamin A/C knockout cells, incapable of forming an actin cap and restored upon lamin A/C rescue. Together, our data suggest that ?1 integrin is required for the recognition of spatial ECM cues and that force transmission to the nucleus via lamin A/C is essential for subsequent directional migration.

Round Table Discussion : invited 7en minutes Key Speech on Frontier Reserch
Authors : Csaba Balazsi
Affiliations : Centre for Energy Research, Konkoly – Thege M. str. 29-33, 1121 Budapest, Hungary

Resume : Ceramic structures at the nanometre range have been proven to have improved properties and characteristics that differ from their bulk, allowing for opportunities in novel technological applications. Ceramic layers with some advantages, including a very high surface-to-volume and aspect ratio can be processed by cheap and quick method, that is electrospraying. This presentation reviews results on the electrospinning and electrospraying of various bioceramic layers starting from biogenic raw materials and their potential applications in the biomedical field.

Authors : Donata IANDOLO
Affiliations : INSERM U1059, Campus Santé Innovations, Saint-Priest-en-Jarez, France

Resume : Bioelectricty is reported to be one of the key regulators of morphogenesis in vivo and electrical potentials have been reported to influence a number of physiological processes. Bioelectronics materials and devices are able to interact with living systems (e.g., tissues, organs, cells). They are indeed able to translate an ionic signal into an electronic readout and they are also able to deliver stimuli influencing ion movements therefore affecting specific target functions (e.g., tissue regeneration).

Authors : Dr., Docent Oleksandr .Ivanyuta
Affiliations : Taras Shevchenko National University of Kyiv,64/13, Volodymyrska Str., Kyiv, 01601, Ukraine

Resume : A biosensor based on collagen composite biopolymers nanofibers is found to be effective in identifying physical influences. Biopolymer nanofibers were made by electrospinning method. After optimizing the synthesis parameters, nanofibers biopolymers were obtained, averaged 50-100 nm in diameter, and then modified on the surface of the working electrode. Effect of the intensity of the transducer buffer and the working potential of the current response. The nanofilament-modified electrode to the temperature is optimized to obtain the maximum current response [1]. Effect of the intensity of the transducer buffer and the working potential of the current response. The nanofilament-modified electrode to the temperature is optimized to obtain the maximum current response. The results revealed that the temperature of the collagen composite biopolymer nanofibers had the advantages of rapid reaction, excellent reproducibility, high stability and showed a nonlinear reaction in the dynamic range from 273 K to 373 K with a detection limit of 0.1 K and a high sensitivity of 1 µA·mM−1· cm−2. The proposed strategy based on collagen composite biopolymer nanofibers can be extended for the development of other enzyme-based biosensors. [1] O.Dubok, O.Shynkaruk, E.Buzaneva, Lanthanides oxides usage to increase radiopaque of bioactive ceramics, Funct. Mater. 2013; 20 (2): 172-179.

18:30 Concluding Remark: Symposium R Organizer – Prof. Peter SCHARFF    
Start atSubject View AllNum.Add
FRONTIER RESEARCH IN NANOMEDICINE Key and Invited Presenters : Session Organizer - Professor Peilin Chen, Academia Sinica, Taiwan
Authors : Peilin Chen
Affiliations : Research Center for Applied Sciences, Academia Sinica, Taiwan.

Resume : In this study, we employed a novel one-step electrospinning process to fabricate poly(ethylene oxide) (PEO)/poly(3,4-ethylenedioxythiophene): polystyrenesulfonate (PEDOT:PSS) core/shell nanofiber structures with improved water resistance and good electrochemical properties. We then integrated a biocompatible polymer coating with three-dimensional (3D) PEDOT-based nanofiber devices for dynamic control over the capture/release performance of rare circulating tumor cells (CTCs), as well as the label-free detection by using organic electrochemical transistors (OECTs). The detailed capture/release behavior of the circulating tumor cells was studied using an organic bioelectronic platform comprising PEO/PEDOT:PSS nanofiber mats with 3 wt % (3-glycidyloxypropyl)trimethoxysilane as an additive. We have demonstrated that these nanofiber mats deposited on five-patterned indium tin oxide finger electrodes are excellent candidates for use as functional bioelectronic interfaces for the isolation, detection, sequential collection, and enrichment of rare CTCs through electrical activation of every single electrode. This combination behaved as an ideal model system displaying a high cell-capture yield for antibody-positive cells while resisting the adhesion of antibody-negative cells. Taking advantage of the electrochemical doping/dedoping characteristics of PEDOT:PSS materials, the captured rare cells could be electrically triggered release through the desorption phenomena of PLL-g-PEG-biotin on the device surface. More than 90% of the targeted cancer cells were captured on the 3D PEDOT-based nanofiber microfluidic device; over 87% of captured cancer cells were subsequently released for collection; approximately 80% of spiked cancer cells could be collected in a 96-well plate. For the OECT design, it was demonstrated for monitoring CTC-capture performance and identifying cancer cell phenotypes. This 3D PEDOT-based bioelectronic device approach appears to be an economical route for the large-scale preparation and detection of systems for enhancing the downstream characterization of rare CTCs.

Authors : Fuyuhiko Tamanoi
Affiliations : Institute for Integrated Cell-Material Sciences, Institute for Advanced Study, Kyoto University

Resume : Our attempt to develop a new type of radiation therapy by using gadolinium-loaded nanoparticles and synchrotron-generated monochromatic X-rays will be discussed.

Authors : Michihiro Nakamura
Affiliations : Department of Organ Anatomy & Nanomedicine, Yamaguchi University Graduate School of Medicine

Resume : Medical applications of multifunctional nanoparticles are expected to be one of the most important possibilities in innovative medicine. Organosilica nanoparticles are novel nanomaterials that are prepared from a single organosilicate coupling agent (organotrialkoxysilane) such as 3-mercaptopropyltrimethoxysilane. Organosilica nanoparticles are both structurally and functionally different from typical silica nanoparticles (inorganosilica nanoparticles) prepared from tetraalkoxysilane. The organosilica nanoparticles contain both interior and exterior functionalities such as mercaptopropyl residue as prepared. The organosilica nanoparticles allow for facile surface and internal functionalization, offering new opportunities to create multifunctionalized nanoparticles. Over the last two decades, research on the internal functionalization of organosilica nanoparticles has evolved. Various sizes of fluorescent organosilica nanoparticle containing various types of fluorescent dye including near infrared (NIR) dye can be prepared using a one-pot synthesis. In addition, functional fusions of organosilica nanoparticles and other functional nanoparticles such as quantum dots, gold nanoparticles, and iron oxides are possible based on organosilica particles technology. These multifunctionalized organosilica nanoparticles are useful for various imaging techniques such as in vivo imaging, cell labeling, time-lapse fluorescent imaging, and multimodal imaging. Multifunctionalized organosilica nanoparticles have high potential to create novel imaging systems and provide novel information of cell characteristics and functions. In recent year, we have launched additional research on surface functionalization of organosilica nanoparticles using biomolecules and polymers. Surface-functionalized organosilica nanoparticles revealed various alterations of the interaction with cells including tumor cells and macrophages. We applied multifunctionalized NIR organosilica nanoparticles to tumor-bearing mouse. The particles showed an accumulation on tumor tissue on NIR in vivo imaging, and damaged tumor cells by using photodynamic effect. Imaging and therapy using multifunctionalized organosilica nanoparticles allow for nano-theranostics.

Authors : Mi Hyeon Cho, Yan Li, Hyunjin Kim, Yongdoo Choi
Affiliations : National Cancer Center

Resume : My laboratory has been developing various types of activatable and dual-targeted photosensitizing agents as smart theranostics for selective near-infrared fluorescence imaging and photodynamic therapy (PDT) of cancers and inflammatory diseases. Recently a fucoidan-based theranostic nanogel (CFN-gel) consisting of a fucoidan backbone, redox-responsive cleavable linker, and photosensitizer was developed to achieve activatable near-infrared fluorescence imaging of tumor sites and an enhanced PDT to induce the complete death of cancer cells. A CFN-gel has nanomolar affinity for P-selectin and VEGF. Moreover, a CFN-gel is non-fluorescent and non-phototoxic upon its systemic administration due to the aggregation induced self-quenching in its fluorescence and singlet oxygen generation. After internalization into cancer cells and tumor neovascular endothelial cells, its photoactivity is recovered in response to the intracellular redox potential, thereby enabling selective near-infrared fluorescence imaging and an enhanced PDT of tumors. It also provides a significant antitumor effect in the absence of light treatment in vivo. Our study indicates that a fucoidan-based theranostic nanogel is a new theranostic material for imaging and treating cancer with high efficacy and specificity.

10:10 Coffee Break    
Authors : I-Chun Chen, Ting-Yu Lu, Jiashing Yu
Affiliations : National Taiwan University, Taiwan

Resume : The native tissues are complex structures consisting of different cell types, extracellular matrix materials, and biomolecules. Traditional tissue engineering strategies have not been able to fully reproduce biomimetic and heterogeneous tissue constructs because of the lack of appropriate biomaterials and technologies. However, recently developed three-dimensional bioprinting techniques can be leveraged to produce biomimetic and complex tissue structures. To achieve this, multicomponent bioinks composed of multiple biomaterials (natural, synthetic, or hybrid biomaterials), different types of cells, and soluble factors have been developed. In addition, advanced bioprinting technologies have enabled us to print multimaterial bioinks with spatial and microscale resolution in a rapid and continuous manner, aiming to reproduce the complex architecture of the native tissues. In this study, we developmented a new formulation of bio-ink which is based on methacrylated keratin and methacrylated glycol chitosan. The feasibility of this bioink was tested with human adipose-derived stem cell toward specific differentiation conditions.

Authors : Hideaki Yamamoto1, Takuma Sumi1, Shigeo Sato1, Ayumi Hirano-Iwata1,2
Affiliations : 1. Research Institute of Electrical Communication, Tohoku University, Sendai, Japan; 2. WPI-Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, Sendai, Japan.

Resume : Nerve-cell culture takes an irreplaceable role in molecular and cellular neuroscience. However, its use in studies at the systems level has been limited due to the substantial difference between in vivo in vitro network dynamics. The dynamics is affected by the difference in the intercellular connectivity (Yamamoto et al., Sci. Adv. 4, eaau4914 (2018)), as well as by the excessively strong excitatory synapses in cultured neurons. Recently, a study was reported that stiff scaffolds enhance the strengths of excitatory synapses in cultured hippocampal neurons (Zhang et al., Sci. Rep. 4, 6215 (2014)). As neuronal cultures are generally performed on polystyrene or glass, which is approximately 10^6 to 10^7 times stiffer than the brain tissue, we hypothesized that the in vitro artifact in excitatory synaptic strength can be reduced by using a scaffold that mimics the elasticity of the brain tissue. Here we employed an ultrasoft silicone elastomer, whose elastic modulus resembles that of the brain tissue (~0.5 kPa), as a scaffold for culturing rat cortical neurons. We investigated the effect of the biomimetic elasticity on the strength of excitatory synapses and the spontaneous network activity (Sumi et al., arXiv 1912.05050 (2019)). We found that the amplitude of excitatory postsynaptic currents was smaller on softer scaffolds. Although globally synchronized bursting activity in cortical cultures were still observed when the cells were grown on the ultrasoft substrate, neuronal correlation was significantly reduced as compared to the cultures on stiffer (>14 kPa) substrates. In the latter half of the talk, we will show how the multielectrode array devices with the ultrasoft cell-device interface can be fabricated by taking advantage of additive manufacturing technologies, such as inkjet printing (Yamamoto et al., Adv. Biosys. 3, 1900130 (2019)). Our device employs 3D micropillar electrodes, which can be fabricated relatively easily by inkjet printing. Such devices facilitate the stimulation and recording of cultured neuronal networks grown on biomimetic scaffolds, for both basic research and pharmacological studies.

Authors : Shih-En Chou, Ji-Yen Cheng
Affiliations : Research Center for Applied Sciences, Academia Sinica Taiwan

Resume : Cell adhesion is a requisite stage in a number of physiological and pathological processes, such as cell differentiation, immune responses, and tumor metastasis. The process of cell adhesion is composed of three steps, cell-substrate contact, cell spreading, and cytoskeleton reorganization. Conventional adhesion assays, such as colorimetric and fluorometric detection, are time-consuming and insensitive. Tracking of cell morphological changes is often difficult to quantify. In the past decades, surface plasmon resonance (SPR) is widely applied in biosensing technology due to the rapid, real-time and label-free characteristics. Here, we developed a novel metal nanoslit-based biosensor with a detection platform of transmissive surface plasmon resonance (t-SPR). The platform can determine cell adhesion by Fano resonance signals, which are changed during cell binding to the nanoslit. Therefore, we can simultaneously analyze the focal adhesion and cell spreading through the spectral peak and dip of the Fano resonance. The peaks and dips reflect the long- and short-range cellular changes, respectively. We previously examined the features of cell adhesion and found that this method has great potential for estimating the thickness of adherent cells and the metastatic potencies of lung cancer cells. Metastasis, a complicated process in which cancer cells spread from a primary tumor to distant sites via the circulatory system, is the main cause of death in cancer patients. In light of this fact, we are now screening commercial drugs like ion-channel inhibitors and G protein-coupled receptors (GPCR) compounds which are related to cell adhesion and might involve in metastasis. The screening could reveal candidates which may be used as anti-metastasis drugs.

Authors : Daisuke Miyoshi, Kazuki Kohata, Wataru Sugimoto, Katsuhiko Itoh, Keiko Kwauchi
Affiliations : Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, 650-0047, Japan

Resume : Molecular environments inside cells are surprisingly crowded with numerous number of biomolecules. About 40 percent of the cell volume is occupied by biomolecules. Biochemical reactions are subject to be temporally, spatially, and specifically controlled. From biochemical and biophysical point of views, it is incredible that selective interactions and specific functionalization of biomolecules in the temporal and spatial-specific manner can be achieved under the complex molecular crowding environments. One of the key futures to achieve the specific interaction of biomolecules is collective biomolecular behavior. In recent years, biomolecular localization and compartmentation systems using droplets via liquid-liquid phase separation (LLPS) inside cells, become one of the most hot research topics in biology. Droplets are temporarily formed, and their formation is reversible and responsive to various external signals, which are in contrast with aggregation of biomolecules which is generally irreversible. In this presentation, we will show a model system of a droplet in a test tube, in which we use nucleic acids (RNA and DNA) which forms G-quadruplexes and arginine-rich peptides which do not have any stable structure. By mixing these two components, a rapid LLPS was observed. It was suggested that the G-quadruplex structure is critical for undergoing LLPS. In the talk, we would like to discuss property of the droplet as a biomaterial.


Symposium organizers
Emmanuel STRATAKISInstitute of Electrnic Structure and Laser (IESL)

Foundation of Research and Technology Hellas (FORTH) and University of Crete, Nikolau Plastira 1000, Voutes, Heraklion, Crete

+30 2810 3912 74
Eugenia BUZANEVATaras Shevchenko National University of Kyiv

NASU “Physical and Chemical Material Science Centre”, Volodymyrs'ka Str. 64/13, 01601 Kyiv, Ukraine

+38 044 294 26 22
Insung S. CHOI (Main)The Center for Cell-Encapsulation Research, KAIST

Dep. of Chemistry and Dep. Bio and Brain Engineering - 281, Daejeon 34141, Korea

+82 42 350 2880
Peter SCHARFFTechnical University of llmenau

Institute of Chemistry and Biotechnology, Weimarer Strasse 25, 98693 Ilmenau, Germany

+49 36 77 69 3603(04)
Thomas J. WEBSTER Northeastern University

Department of Chemical Engineering - Center Advanced Materials Research - 313 Snell Engineering Center - Boston, IMA 02115, USA

+1 617 373 6585