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Biomaterials and soft materials


Bioinspired and biointegrated materials as new frontiers nanomaterials

This symposium is composed of SPECIAL SESSIONS covering hot topics such as biomimetic materials, supramolecular nanomaterials and smart materials. These materials find applications in a range of fields encompassing smart living building and human health.


The newest research ideas and nanotechnologies from smart (nano)materials, integrated systems to robotic devices will be presented. The covered themes include tissue and organ regeneration (e.g. skin, bone tissue and neural networks) as well as implantable bionic systems.

These systems can include, but is not limited to, molecular systems, nanomaterials (templated by biomolecules such as viruses, marine plants, proteins, pigments), nanoparticles as nanorobots for in vivo applications, supramolecular complexes, inorganic NPs.

The symposium will bring together researchers from chemical, physical sciences and bio - science and – nanotechnology biomaterials for nanomedicine and engineering bio - electronic, - photonic, - magnetic nanosystems to discuss the latest advancements.

Proposed subjects for discussions at this event have actuality for Investigators - Collaborators of the EU COST Actions, as and the EU HORIZON 2020-2025 Projects.

A special Young Scientist FORUM (hosting Post-Graduate, PhD and Graduate students’ talks) will be held at the symposium’s second day.

Hot topics to be covered by the symposium:

  1. Design and synthesis of (supramolecular) materials and their action in  bio – mimetic material synthesis;
  2. Bioinspired inorganic, smart nanoparticles (NPs);
  3. Design and creation of smart supramolecular materials and their biohybrids;
  4. Novel concepts in nano - characterization, bio-recognition of smart and specially bio-hybrid stimuli responsive nanomaterials with applications for clinical, food and environmental monitoring;
  5. Molecularly imprinted materials;
  6. Smart, biomimetic materials as scaffolds for tissue engineering;
  7. Electronic/photonic/magnetic smart biomolecules (nucleic acids, virus, marine plants proteins, pigments) and their mimetic analogues;
  8. Neuroelectronics;
  9. Biosensing devices for medical (in vivo and in vitro diagnostics) and environmental biotechnologies: developed 3rd generation biosensors, carbon 2D materials, bioelectronic textiles, printed paper electronics, electronic skin);

Invited partners:

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08:50 Opening remarks & Symposium Presentation Prof.Insung S. CHOI    
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08:45 Plenary Session Lecture (08. 45 – 09.45) by Professor Sir James Fraser Stoddart, Nobel Laureate in Chemistry (2016), Northwestern University, USA    
08:45 The Nobel Prize in Chemistry 2016 was awarded jointly to Jean-Pierre Sauvage, Sir J. Fraser Stoddart and Bernard L.Feringa “for the design and synthesis of molecular machines”    
YOUNG INVESTIGATOR FORUM : Invited Organizers/Chairs - PhD, Post-Doctoral Researchers Nanasaheb D.Thorat (Poland) and Katharina Brassat (Germany), PhD student Toshifumi Imajo (Japan)
Authors : Nanasaheb D. Thorat, Joanna Bauer
Affiliations : Department of Biomedical Engineering, Faculty of Fundamental Problems in Technology, Politechnika Wroclawska, Wroclaw, Poland

Resume : Countless efforts are made by scientist to design different type of nanostructures, in the investigation for next generation cancer care applications. Various nanostructures often employ multimodality and show encouraging results at preclinical stage in cancer therapy. Specially designed smart nanostructures such as hybrid nanostructures are responsive to external physical stimuli such as light, magnetic field, electric, ultrasound, radio frequency, X-ray, etc are currently being investigated for cancer therapy. Thus, hybrid nanostructures provide a unique combination of important properties to address key challenges in modern cancer therapy: (i) an active tumor targeting mechanism of therapeutic drugs driven by a physical force rather than passive antibody matching, (ii) an externally/remotely controlled drugs on-demand release mechanism, and (iii) a capability for advanced image guided tumor therapy and therapy monitoring. The present report/talk cover various type of nanostructures that are developed by our group and those are responsive to magnetic and light-triggered modalities currently being developed for nonconventional cancer treatments. The physical basis of nanostructures and stimuli responsive modality is explained; so audience with a physics or, materials science background can easily grasp new developments in this field.

Invited Presentations : The 7en minutes Report on Frontier Research
Authors : Toshifumi Imajo, Takashi Suemasu, Kaoru Toko
Affiliations : Univ. of Tsukuba

Resume : Ge has higher carrier mobility than conventional mainstream material Si, and also has a low crystallization temperature (< 500 °C), which can be synthesized onto insulators under the heatproof temperature. Therefore, Ge on insulator technology has been widely studied for lowering the fabrication cost and improving the device performance. In particular, Ge-based devices fabricated on flexible plastic substrates will open up the possibility for developing advanced wearable devices such as multi-functional display. Solid phase crystallization (SPC) is a simple method to directly form polycrystalline Ge (poly-Ge) thin films on insulating substrates at low temperatures. Recently, we found that the control of the atomic density of an amorphous Ge precursor for SPC dramatically enlarged the grain size and updated the highest record of hole mobility of semiconductor thin film on insulator [1,2]. In this study, we applied this method onto plastic substrate. As a result, the SPC-Ge on a plastic substrate exhibited higher hole mobility than that of bulk Si. This result mean that single-crystal wafers are no longer necessary for fabricating a high-mobility semiconductor thin film. Besides, the resulting hole mobility 680 cm2/Vs is the highest value to date among those of semiconductor layers directly formed on insulators at low temperatures. This achievement will give a way to realize advanced electronic and optical devices simultaneously allowing for high performance, inexpensiveness, and flexibility. [1] K. Toko et al., Sci. Rep. 7, 16981 (2017). [2] T. Imajo et al., Applied Physics Express 12, 015508 (2019).

Authors : M. Marini,1,2 S. Stassi,1 M. Allione,2 B. Torre,2 A. Giugni,2 M. Moretti,2 P. Zhang,2 C.F. Pirri,1 C. Ricciardi,1 E. Di Fabrizio, 2
Affiliations : 1 DISAT, Politecnico di Torino, Corso Duca Degli Abruzzi, 24, 10129 Torino, Italy. 2 PSE Division, KAUST, Thuwal 23955, Saudi Arabia.

Resume : Recently we obtained free-standing self-assembled biomolecules fibers, by using a physiological-compatible preparation and super-hydrophobic surfaces (SHS). A droplet of the biomolecule solution is deposited over the SHS. At room temperature, the water in solution evaporates: the droplet decreases in volume and moves from one pillar to the next. With this method the molecules in solution are pulled and linked between micro-pillars. We suspended DNA, DNA/ligands, and cell membranes that were characterized by EM, Raman Spectroscopy and Laser Doppler Vibrometer. The DNA fibers allowed us obtaining background-free TEM direct images and measuring DNA bases and backbone without the use of contrast agents, with a resolution of 1.5 Å. TEM diffraction confirmed the quantities measured. Raman spectroscopy data expanded the EM data with structural/chemical information on DNA monomers, conformation and fluctuations related to the environment. DNA bundles were also used as ultrasensitive mechanical resonators to detect and study deviations to the native form, by administering intercalants and the chemotherapy cisplatin at increasing concentration. Alteration to sizes and Young’s modulus were successfully quantified. Overall, the results show that our approach can be applied to medical-oriented developments such as the optimal chemotherapy titration and the evaluation of the effects on DNA of pollutants and contaminants such as heavy metals.

Authors : Qun Ma, Pengcheng Gao*, Fan Xia*
Affiliations : Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences (CUG), 388 lumo Road, Wuhan 430074, R. P. China.

Resume : During the decades, widespread advances have been achieved on nanochannels correspondingly, including nanochannel based DNA sequencing, single-molecular detections, smart sensors, energy transfer/storage and so on. However, researchers focus all interests on the contribution from the functional elements (FEs) at the inner wall (IW) of nanochannels, little attention has been paid on the contribution from the FEs at the outer surface (OS) of nanochannels. Herein, we achieve explicit partition of FEOS and FEIW based on accurate regional-modification of OS and IW. Furthermore, the FEIW are served for ionic gating, and the chosen FEOS (hydrophobic or charged) are served for blocking interference molecules into the nanochannels, decreasing the false signals for the ionic gating in complex environments. Furthermore, we also define a composite factor, areas of a radar map, to evaluate the FEOS performance for blocking interference molecules.

Authors : Christopher Jay T. Robidillo,1,2 Jonathan G. C. Veinot1*
Affiliations : 1Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada 2Department of Physical Sciences and Mathematics, University of the Philippines Manila, Manila 1000, Philippines *Corresponding author

Resume : This study reports the ratiometric photoluminescent detection and quantification of the toxicologically potent organophosphate ester nerve agents paraoxon (PX) and parathion (PT) using red-photoluminescent silicon-based quantum dots (SiQDs) and the green fluorescent protein mAmetrine1.2 (mAm).1 The nerve agents selectively quench SiQD photoluminescence via a dynamic quenching mechanism mediated by organophosphate nitroaromatic groups. The method reported herein afforded micromolar detection limits for PX and PT and is unaffected by inorganic and organic interferents. In addition, paper-based sensors prepared from SiQDs and mAm have been successfully employed for the detection of PX and PT at low concentrations using a color analysis smartphone application. The sensor reported herein can potentially be used in the determination of PX and PT in actual samples. Reference: 1. Robidillo, C. J. T.; Wandelt, S.; Dalangin, R..; Zhang, L.; Yu, H.; Meldrum A.; Campbell, R.E.; Veinot, J. G. C. ACS Appl. Mater. Interfaces 2019, 11, 33478-33488.

Authors : Alina Vladescu1, Jürgen Schmidt2, Pinar Yilgor Huri3, Catalin Vitelaru1, Dorit Kloss2, Nesrin Hasirci4,6, Vasif Hasirci5,6, Lidia R. Constantin1, Mariana Braic1
Affiliations : 1National Institute of Research and Development for Optoelectronics - INOE 2000, 409 Atomistilor St., 077125, Magurele, Romania 2Innovent e.V., Jena, Prüssingstraße 27B, 07745 Jena, Germany 3Ankara University, Department of Biomedical Eng., Golbasi, Ankara, Turkey 4Near East University, Nicosia, Mersin 10, Turkey 5Acibadem Mehmet Ali Aydinlar Univ., Dept. Med. Eng., Istanbul, Turkey 6BIOMATEN, Middle East Technical University, Ankara 06100, Turkey

Resume : The growing implant market requires advanced Ti-based implants for long-term application, and for the newly engineered biodegradable Mg alloys with controllable dissolution rate s short-term applications. For this reason, Mg-alloys gain much more attention recently. Mg alloys are biodegradable material, but with too high corrosion rate and degradation occurs before the end of healing process. Moreover, during the corrosion, hydrogen released thereby causes pH increase of the surrounding tissue, inducing apoptosis and necrosis of tissue cells. For these reasons, it is a challenge to controlled degradation rate of Mg alloy and to provide sufficient time for the tissue to heal up to complete degradation of Mg implant. The aim of the present paper is to investigate different types of CaP coatings as possible candidate for temporary implants made of MgCa1 alloy. The coatings were prepared by magnetron sputtering and micro-arc oxidation methods. The coatings were investigated in terms of microchemical, microstructural and mechanical properties, corrosion resistance in SBF at 37°C, as well as the biocompatibility with human cell line. We acknowledge the support of the Romanian projects: no. COFUND-ERANET-RUS-PLUS-CoatDegraBac (no. 68/2018), within PNCDI III; Core Program-2020; no. 19PFE/2018 (PROINSTITUTIO).

Authors : Ph.D student Alexina Ollier1,2, Marcin Kisiel1, Urs Gysin1 and Ersnt Meyer1
Affiliations : 1 Institute of Physics, University of Basel, Switzerland 2 Swiss Nanoscience Institute, Klingelbergstrasse 82, 4056 Basel, Switzerland

Resume : Understanding nanoscale energy dissipation is nowadays among few priorities particularly in solid state systems. Breakdown of topological protection, loss of quantum information and disorder-assisted hot electrons scattering in graphene are just a few examples of systems, where the presence of energy dissipation has a great impact on the studied object. It is therefore critical to know, how and where energy leaks. Pendulum geometry Atomic Force Microscope (pAFM), oscillating like a pendulum over the surface, is perfectly suited to measure such tiny amount of dissipation, since a minimum detectable power loss is of the order of aW. Here, we report on a low temperature (T=5K) measurement of striking singlets or multiplets of dissipation peaks above graphene nanodrums surface. The stress present in the structure leads to formation of few nanometer sized graphene quantum dots (GDS) and the observed dissipation peaks are attributed to tip-induced charge state transitions in quantum-dot- like entities. In the real space the charging features are observed as Coulomb rings surrounding the GDS. The dissipation peaks and Coulomb rings strongly depend on the external magnetic field (B=0T-2T), the behavior we attributed to crossover from quantum dot carrier confinement to the confinement by magnetic field.

Authors : A.A. Romansky, V.L. Karbivskyy, V.А. Dubok, S.S. Smolyak, L.I. Karbivska
Affiliations : G.V. Kurdyumov Institute for Metal Physics of the N.A.S. of Ukraine

Resume : Previously, we developed samples of biocompatible composite based on calcium phosphates – "Syntekost" (synthetic bone). The aim of this work is to optimize the properties of bioactive materials for bone tissue recovery – ensuring their oncoprotective and anti-inflammatory properties. At various temperature conditions, a number of inorganic materials were synthesized: calcium phosphate ceramics, glasses and sitalls, as well as composites based on nanodispersed calcium hydroxyapatite. The effect of synthesis temperature conditions on the structure and properties of the obtained materials is investigated. Doping of a number of samples with bioactive elements was carried out with the aim of their functionalization. Samples of conductive bioactive nanocomposites based on nanodispersed apatite and nanodispersed graphite were obtained, and their properties were studied by electrophysical methods. An analysis of the XPS data of the obtained samples showed that, depending on the conditions of glass synthesis – melt cooling rate, temperature regime, and glass doping, the binding energy of the constituent elements changes. The temperature conditions of the process for producing such materials were optimized. The relative fraction of bioactive calcium phosphates and their crystallinity correlate with synthesis conditions, in particular – temperature regime. The XPS data for the fused glass sample indicate the presence of CaSiO_3 and Mg_2SiO_4, which are associated with bio-sitalls.

Authors : Siaka Fadera, I-Chi Lee
Affiliations : Chang Gung University

Resume : The existence of cancer stem cells (CSCs) has been demonstrated in several solid tumors including brain cancer. These CSCs represent a sub-population of cells within tumor bulk that exhibit stem-cell-like characteristics and they are believed to play a significant role in tumor formation and recurrence, metastasis and they are resistant to conventional chemotherapeutic drugs. They are enriched in stem cells markers and markers associated with higher drug resistance and self-renewal. Chemotherapy is a well-accepted treatment modality for different cancer types including glioblastoma, however, its efficacy on glioblastoma still remains far from the best in most cases. The chemotherapeutic drugs are mostly effective on normal cancer cells leaving glioblastoma (GBM) stem-like cells insignificantly affected after treatment. Therefore, the establishment of better treatment strategy is needed in order to combat glioblastoma stem-like cells drug resistance. One such strategy is the induction of differentiation on GBM CSCs to normal cancer cells that will circumvent their self-renewal ability and improve their sensitivity to anti-cancer drugs. Previous literature has used low-intensity ultrasound (LIPUS) to inhibit the proliferation and induce apoptosis in cancer cells. Additionally, we have reported the induction effect of LIPUS on liver CSCs. In the present study, LIPUS was deigned to induce differentiation and concomitantly enhance the sensitivity and an uptake of drug by CSCs isolated from GBM cell line. We first designed and developed three dimensional tumor microenvironment using hyaluronic acid (HA) based polyelectrolyte multilayer (PEM) film system in order facilitate the isolation and enrichment of GBM CSCs. The next step is the use of LIPUS stimulation of GBM CSCs in combination with anti-cancer loaded gold nanoparticles. Colony formation, migration and invasion, putative CSCs marker expression, drug resistance, stem cell related genes expression and immunostaining were all investigated and compared with control group. The results revealed that high colony formation was observed on PEM system after 3 days of culture which continued through 7 days after culture. Higher expression of CD133, higher migration and invasion ability, up-regulation of proteins and genes related to CSCs and an increased drug resistance capacity were observed in cells isolated on PEM film system. From our previous study, LIPUS stimulation may result to down- regulation of CSCs colonies, down-regulation of CSCs related proteins and genes, down-regulation of migration and invasion ability, and an enhanced drug sensitivity of CSCs. Our proposed study design may provide alternative therapeutic strategy on the differentiation and effective combination therapy of glioblastoma CSCs.

Authors : Alina Sharova, Mario Caironi
Affiliations : Dipartimento di Fisica, Politecnico di Milano, P.zza L. da Vinci 32, 20133 Milan, Italy; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia, Via Pascoli 70/3, 20133 Milan, Italy

Resume : Going beyond the traditional concept of electronic devices, we convey the idea of making electronics edible. This unconventional approach exploits the electronic properties of natural and food-based materials for developing ingestible functional devices. Critical biomedical, pharmaceutical, and food industry applications are targeted by the proposed field. In this framework, we explore the potential of cost-effective and edible substance, honey, to be used as electrolytic gate viscous dielectric. Honey-gated organic field effect transistors (OFETs) based on both n & p type semiconductors are fabricated. A distinctive feature of these transistors is their long-term stability, reproducibility and low voltage < 1V operation in air. Devices exhibit forward-looking electronic performances, notably, electron and hole mobility–capacitance product of 3.5 × 10–3 μF/Vs and 23 × 10–3 μF/Vs, respectively, surpassing ones of the previously reported water-gated OFETs. Furthermore, the observed devices responsivity to humidity provides promising opportunities for sensing applications. We then demonstrate, for the first time, the implementation of honey-based integrated circuits: inverting logic gate and ring oscillator.

Authors : Francesca Guagnini, Peter B. Crowley
Affiliations : School of Chemistry, National University of Ireland, Galway, Ireland

Resume : Thanks to their versatility, proteins are ideal building blocks for functional materials. Controlled protein assembly is necessary for bottom-up fabrication of biomaterials.[1] Many strategies can be employed to achieve protein assembly, like computational design of protein interfaces, supercharged proteins or tethered linkers.[1] The use of macrocyclic receptors is a viable alternative. Macrocyclic receptors, like cucurbiturils or calixarenes, recognize patches on protein surfaces and induce assembly / crystallization by bridging two or more proteins. This transferable approach has been demonstrated in different proteins and does not require expensive protein functionalization or the use of designed proteins.[2-3] Metal coordination is another directional interaction employed to facilitate protein assembly.[4] We postulated that macrocycle recognition coupled with metal coordination will increase the control over assembly design. To demonstrate this idea, we tested two different protein model systems: a β-propeller lectin and a highly cationic small protein. Our research paves the way for new biohybrid materials. [1] Luo, Q.; Hou, C.; Bai, Y.; Wang, R.; Liu, J., Chem. Rev., 2016, 116, 13571–13632. [2] Rennie, M. L.; Fox, G. C.; Pérez, J.; Crowley, P. B., IUCRJ, 2019, 6, 238-247. [3] Guagnini, F.; Antonik, P. M.; Rennie, M. L.; O’Byrne, P.; Khan, A. R.; Pinalli, R.; Dalcanale, E.; Crowley, P. B., Angew. Chemie Int. Ed., 2018, 57, 7126–7130. [4] Bailey, J. B.; Zhang, L.; Chiong, J. A.; Ahn, S.; Tezcan, F. A., J. Am. Chem. Soc., 2017, 139, 8160–8166.

GENERAL POSTER SESSION : Chairs: Insung S. Choi (Korea), Peter Scharff (Germany), Emmanuel Stratakis (Greece), Thomas J. Webster (USA), Eugenia Buzaneva and Oleksandr Ivanyuta (Ukraine)
Authors : R.M. Dodo1, M. Abdulwahab1,2, U. Shehu1, F. Asuke1, A. Hamisu1
Affiliations : 1Department of Metallurgical and Materials Engineering, Ahmadu Bello University, Zaria, Nigeria 2Department of Chemical, Metallurgical and Materials Engineering, Tshwane University of Technology, Pretoria, South Africa

Resume : The present study explored wear and biocompatibility characteristics of polypropylene (PP)/ periwinkle shell particulates (PWS) composites for dental implant application. Wet-sliding the stainless steel ball against specimen disc revealed the abrasive wear behaviour of the composites under load and linear speed of 5N and 10 cm/s respectively. In the wear tests, the specimens were wetted with simulated human saliva with and without sodium fluoride (NaF) additive. On the other hand, a mouse specimen was used to carry out a biocompatibility test. The composite demonstrated superb wear resistance at 40 and 50wt% reinforcement loading. Further, results showed that wear rate decreased considerably with increasing PWS content. Higher wear rate was noted on composites under condition of simulated human saliva with NaF addition. The biocompatibility test reveals that developed composite is harmless to the living tissues. Thus, PP/PWS composites could be recommended to be used as dental restorative material.

Authors : Toshifumi Imajo, Takashi Suemasu, Kaoru Toko
Affiliations : Univ. of Tukuba

Resume : To realize high-performance thin-film transistors, solid-phase crystallization (SPC) of Ge films on insulators has been widely investigated, which is a simple method to directly form polycrystalline thin films on insulators. Recently, we succeeded in increasing the grain size of the Ge thin film on the glass substrate by controlling the density of amorphous Ge, which is the precursor of SPC, and have continued to update the highest record of mobility of the low-temperature synthetic film [1,2]. In this study, we develop this SPC-method onto plastic substrate. In the experiment, GeO2 underlayer was sputtered on both SiO2 glass and plastic substrates. After that, amorphous Ge precursors were prepared by molecular beam while heating the samples at 150 °C. Finally, the samples were then loaded into a conventional tube furnace in a N2 atmosphere and annealed (375 °C, 150 h) to induce SPC. As a result, SPC-Ge formed on a plastic has almost the same crystal grain size as that on a glass. By examining the electrical properties of substrate dependence of Ge layer, we found that there is almost the same tendency of electrical properties between the samples on plastic and glass substrates. With the above, we performed post annealing (500 °C, 5 h) in order to reduce the grain boundary barrier and the impurity scattering, and achieving hole mobility of 680 cm2/Vs for Ge thin film on plastic substrate. It is the highest value to date among those of semiconductor layers directly formed on insulator and is a result for exploiting excellent flexible devices. [1] K. Toko et al., Sci. Rep. 7, 16981 (2017). [2] T. Imajo et al., Applied Physics Express 12, 015508 (2019).

Authors : Pengxiang Si, Fan Jiang, Qingsha S. Cheng*, Geoffrey Rivers, and Boxin Zhao*
Affiliations : P. Si, Dr. G. Rivers, Prof. B. Zhao Department of Chemical Engineering Waterloo Institute for Nanotechnology University of Waterloo 200 University Avenue West, N2L 3G1, Canada F. Jiang, Prof. Q. S. Cheng Department of Electrical and Electronic Engineering Southern University of Science and Technology 1088 Xueyuan Ave, 518055, China

Resume : Antennas are an essential component of next generation wearable electronics for wireless communication. It is highly desirable to develop a robust self-healable antenna to maintain the integrity and function of the device because the wearable devices are becoming ever-thinner and smaller, making them subject to serious wear and damage during the body bending or stretching. However, it has been a significant material challenge as the integral antenna can not be exposed to external stimulus such as heat, light and chemical agents without damaging the devices. Herein, we design a latex-polyelectrolyte coacervation (LPC) system compounded with conductive filler to form a printable conductive paste which exhibits robust toughness, stiffness, extensibility, high electrically conductivity, one-step printability and self-healing capability simultaneously. The formation of polymer network via electrostatic interaction and hydrophobic interaction within antenna provide a self-healing mechanism via the absorption of moisture in the ambient air. The LPC system is promising for broad applications in self-healable stretchable electronics.

Authors : Oleksandr Ivanyuta1, Eugenia Buzaneva2, Uwe Ritter3, Peter Scharff3
Affiliations : 1 Faculty of Radiophysics Electronics and Computer Systems, Taras Shevchenko National University of Kyiv, Ukraine E-mail: 2 The Scientific and Training Center on Physical and Chemical Material Science, National Taras Shevchenko National University of Kyiv and NAS of Ukraine 3 Institute of Chemistry & Biotechnology, T U Ilmenau, Germany

Resume : Biofunctionalization of carbon nanotubes, which are well-known for their unique complex of properties, is in the centre of research attention for numerous biomedical applications, also as for fundamental study of nanotubes as templates. Covalent or non-covalent immobilization of biomolecules on carbon nanotubes opens the doors for novel biocomposite manufacturing and biosensing. Carbon nanotube functionalization by DNA, RNA, oligonucleotides or single nucleotides is of special interest as possible approach for nucleic acid sensing, gene delivery, gene therapy, clinical diagnosis and pathogen monitoring. It is also used in nanotechology as technique for suspensioning, characterization and separation of nanotubes. In our investigations the non-covalent immobilization in aqueous suspensions of AMP and DNA on multi-walled carbon nanotubes developed. Previous oxidation of multi-walled carbon nanotubes by ozonolysis were used if it was necessary. The characterization of functionalized nanotubes was carried out by Scanning Electron Microscopy, Transmission Electron Microscopy, Optical Microscopy, Raman, UV-visible-NIR, IR- and Photoluminescence Spectroscopies. Various models of interface formation between biomolecules and nanotube surface are discussed. Optical characteristics of novel biofunctionalized nanotubes also gave us necessary information for their future application as biosensors or activating agents.

Authors : Monica Marini 1, Bruno Torre 2, Marco Allione 2, Maria Caterina Morello 2, Manola Moretti 2, Andrea Giugni 2, Enzo di Fabrizio 2
Affiliations : 1 DISAT, Politecnico di Torino, Corso Duca Degli Abruzzi, 24, 10129 Torino, Italy. 2 SMILEs Lab, PSE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia

Resume : Oriented and self-organized DNA filaments were obtained by micro-fabricated super-hydrophobic surfaces (SHS). The bio-macromolecule, suspended over the SHS, can be characterized by using several techniques, such as electron microscopy and optical spectroscopy. DNA direct imaging by high resolution TEM (HRTEM) allowed solving the base pairs with a resolution of 1.5 Å [1,2] and the metrological details can be effectively corroborated by Raman spectroscopy data [3,4]. Raman spectra in the range 600-1800 cm-1 were acquired on suspended DNA filaments and on the droplet residual. The spectra obtained at the same working conditions on DNA samples and buffer deposited over a CaF2 window were used as negative control. The study of the spectra revealed the absence of physiologically compatible buffers on suspended filaments while their contribution is strong in the DNA spectra acquired on CaF2 windows and on the droplet residual. This suggests that the optimized SHS platform separates small molecules from the suspended DNA and the non-interacted material is concentrated in the droplet residual. The SHS-DNA platform revealed a strong potential for the study of polarized Raman spectra as the DNA filaments are autonomously oriented with different angles over the device and for the analysis of the presence and influence of molecules affecting the DNA double helix such as chemotherapeutic compound (Cisplatin), heavy metals and methylations.

Authors : Alexina Ollier1,2, Marcin Kisiel1, Urs Gysin1 and Ernst Meyer1
Affiliations : 1 Institute of Physics, University of Basel, Switzerland 2 Swiss Nanoscience Institute, Klingelbergstrasse 82, 4056 Basel, Switzerland

Resume : Understanding nanoscale energy dissipation is nowadays among few priorities particularly in solid state systems. Breakdown of topological protection, loss of quantum information and disorder–assisted hot electrons scattering in graphene are just few examples of systems, where the presence of energy dissipation has a great impact on the studied object. It is therefore critical to know, how and where energy leaks. High sensitivity pendulum geometry Atomic Force Microscope (AFM), oscillating like a pendulum over the surface, is perfectly suited to measure tiny amount of dissipation. The tip position on the sample is controlled with atomic accuracy owing to a tunneling current line and the enhanced sensitivity allows to distinguish between electronic, phononic or van der Waals types of dissipation. Measurements can be performed in a wide range of temperatures from 5K to room temperature and in magnetic fields spanning from B=0T to B=7T. The design of the sample holder allows to perform dissipation measurements while passing electric current in the plane of the sample surface. In this work we performed energy dissipation measurements on a suspended graphene sheet at room temperature under UHV. The graphene is deposited on a hole patterned substrate in order to have suspended circular (with a diameter of 6.5 µm) graphene sheet. The experiments allows to investigate the phononic and electronic energy dissipation of the suspended graphene.

Authors : Happy Agarwal; VenkatKumar Shanmugam*
Affiliations : School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore – 632014, TN, India *Corresponding Author Mail id:;

Resume : In the present study, we have biosynthesized ZnO NPs and investigated the mechanism of anti-inflammatory action of the nanoparticles in both in vitro and in vivo models. In the in vitro model system, RAW 264.7 murine macrophages were pre-incubated with ZnO NPs and the effect of NPs on the lipopolysaccharide (LPS)-induced activation of nuclear factor-kappa B (NF- κB) pathway was evaluated. Results show that LPS mediated increase in expression NF- κB pathway was downregulated by an increase in expression of inhibitor of kappa B alpha (IκBα) in NP treated cells in a dose-dependent manner. The anti-inflammatory potential of ZnO NPs was also evaluated in Carrageenan induced mice paw edema model. Administration of ZnO NPs to mice, prevented Carrageenan induced mice paw edema and also suppressed the expression of pro-inflammatory mediators like interleukin-1 beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), and cyclooxygenase-2 (COX-2). Histological analysis of mice paw tissue section clearly indicated a reduction in leukocyte and neutrophil extravasation to the tissue space in mice paw that was pre-treated with ZnO NPs. Present findings give us an insight into the ZnO NPs mediated anti-inflammatory action.

Authors : Soumya Menon, Dr.Venkatkumar S.
Affiliations : School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore 632014, India

Resume : Cervical cancer is the most common malignancy suffered by women worldwide in the female reproductive system. The chemotherapeutic treatment using anticancer drugs are developed recently, but they lack delivery to the targeted tumour tissues. The commonly used anticancer drug paclitaxel is highly efficient against various cancer types, but due to its low solubility the enhancement of its property decreases. Recent advances includes nanotechnology based combinatorial drug delivery systems that offer enhanced pharmacokinetic property due the EPR (enhanced permeable retention), the larger surface area to volume ratio in nanoparticles, it also helps in adsorbing large amount of drug and act as a drug carrier. The combination of nanosystems aids in active or passive drug delivery through the cell membranes via endocytosis. In particular selenium nanoparticles is a well established chemotherapeutic agent due to low toxicity to the normal tissues, when compared to other nanosystems. The selenium nanoparticles act as both prooxidant in the presence of cancer tissues by producing ROS which eventually causes oxidative stress, mitochondrial damage, alter the genetic arrangements of the cancerous tissues, they are also major component of many antioxidant enzymes that also function at protecting from tumour like GSH (glutathione), selenomethioninecystien (recent established amino acid), theoredoxin (Trx) and so on. They act as antioxidant agent for normal tissues by scavenging ROS, regulating the DNA repair proteins, and protecting the damages caused by oxidative stress. In this paper more emphasis is done on the green synthesis of selenium nanoparticles to avoid the utilization of external harmful chemicals. The precursor used for the reduction of the sodium selenite which itself it a harmful chemical which was traditionally used as anticancerous agent, are Mucuna pruriens seed extract which is also a traditional medicine against various cancer types. The extract is used as a bioreductive catalyst which also helps in stabilization of the nanoparticle development. The biosynthesized SeNPs are optimized using BB design for evaluating the optimum particle size and process variable. The optimized SeNPs are then coated with chitosan which acts a biodegradable linker against anticancer drug paclitaxel. The combinatorial nanosystem is evaluated for its anticancerious activity against HeLa cancer cells. This innovative strategy can help in overcoming the drawbacks faced by chemotherapeutic drugs with low solubility, delivery to the targeted sites, highly biodegradable and low toxicity.

Authors : A.A. Romansky, V.L. Karbivskyy, V.А. Dubok, S.S. Smolyak, L.I. Karbivska
Affiliations : G.V. Kurdyumov Institute for Metal Physics of the N.A.S. of Ukraine

Resume : Previously, we developed samples of biocompatible composite based on calcium phosphates – "Syntekost" (synthetic bone). The aim of this work is to optimize the properties of bioactive materials for bone tissue recovery – ensuring their oncoprotective and anti-inflammatory properties. At various temperature conditions, a number of inorganic materials were synthesized: calcium phosphate ceramics, glasses and sitalls, as well as composites based on nanodispersed calcium hydroxyapatite. The effect of synthesis temperature conditions on the structure and properties of the obtained materials is investigated. Doping of a number of samples with bioactive elements was carried out with the aim of their functionalization. Samples of conductive bioactive nanocomposites based on nanodispersed apatite and nanodispersed graphite were obtained, and their properties were studied by electrophysical methods. An analysis of the XPS data of the obtained samples showed that, depending on the conditions of glass synthesis – melt cooling rate, temperature regime, and glass doping, the binding energy of the constituent elements changes. The temperature conditions of the process for producing such materials were optimized. The relative fraction of bioactive calcium phosphates and their crystallinity correlate with synthesis conditions, in particular – temperature regime. The XPS data for the fused glass sample indicate the presence of CaSiO_3 and Mg_2SiO_4, which are associated with bio-sitalls.

Authors : Geun-Tae Yun, Woo-Bin Jung, and Hee-Tae Jung*
Affiliations : Korea Advanced Institute of Science and Technology (KAIST)

Resume : Both high static contact repellency and resistance to liquid drop impact are quite necessary to achieving a high-performance omniphobic surface. The cuticles of springtails, a small insect in nature, have both of these features, which result from their hierarchical structure composed of primary nanostructures on secondary microgrooves. Despite intensive efforts, none of the previous reports that were inspired by the springtail were able to achieve both high static repellency and pressure resistance simultaneously due to a general trade-off between these features. We demonstrate for the first time a springtail-inspired superomniphobic surface showing both features by fabrication of a hierarchical structure consisting of serif-T–shaped nanostructures on microscale wrinkles. Our biomimetic engineering yielded a surface having high repellency to diverse liquids, from water to ethanol, with an apparent contact angle above 150°. The surface was also able to show extreme pressure resistance from the impacts of diverse liquid drops, which is the best pressure resistance on omniphobic surface ever reported.

Authors : Priya Mullick 1 , Gopal Das 2* and Aiyagari Ramesh 1*
Affiliations : 1 Department of Biosciences and Bioengineering, 2 Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Tel: 91-361-2582313; Fax: 91-361-2582349 (Gopal Das) Tel: 91-361-2582205; Fax: 91-361-2582249 (Aiyagari Ramesh) E-mail: (Gopal Das); (Aiyagari Ramesh)

Resume : Herein, we report a biogenic route of hydroxyapatite nanoparticle (HANP) synthesis either in presence of the whole cell (WC) of the lactic acid bacteria (LAB) Lactobacillus rhamnosus GG or a malonic acid amphiphile (MA). HANPs were generated by in situ precipitation and the characteristic peaks at 3568, 1461, and 1041 cm-1 observed in FTIR analysis and the prominent peaks at around 2θ = 26° and 2θ = 33° in PXRD analysis revealed that the LAB cells and MA could generate hydroxyapatite phase. FESEM revealed that WC-HANPs were spindle-shaped and 70-110 nm in size while MA-HANPs were spherical and 22-27 nm in size. HRTEM indicated that the lattice distance for WC-HANPs and MA-HANPs was 0.28 nm and 0.29 nm, respectively, while SAED confirmed the crystallinity of HANPs. TGA analysis indicated degradation of WC-HANPs and MA-HANPs from 20°C to 800°C, with 83% and 87% residual mass, respectively. BET analysis indicated that WC-HANPs and MA-HANPs had a surface area of 170.47 m²/g and 90 m²/g, respectively, and an average pore size of 4.9 nm and 4.04 nm, respectively. Both the HANPs were non-toxic to cultured osteosarcoma MG-63 cells. Fluorescence microscopic analysis revealed that the MG-63 cells grown in presence of HANPs appeared elongated, suggesting that HANPs likely support bone cell differentiation. The biogenic route of HANP synthesis outlined herein is an interesting prospect to generate potentially biocompatible nanomaterials for tissue engineering applications.

Authors : Alina Sharova, Mario Caironi
Affiliations : Dipartimento di Fisica, Politecnico di Milano, P.zza L. da Vinci 32, 20133 Milan, Italy; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia, Via Pascoli 70/3, 20133 Milan, Italy

Resume : The use of natural and bioinspired materials is an emerging key approach towards development of new-generation safe technology. Going beyond the traditional concept of electronic devices, we convey the idea of making electronics edible. This unconventional approach exploits the electronic properties of natural and food-based materials for developing ingestible functional devices. Critical biomedical, pharmaceutical, and food industry applications are targeted by the proposed field. In this framework, we explore the potential of cost-effective and edible substance, honey, to be used as electrolytic gate viscous dielectric. Honey-gated organic field effect transistors (OFETs) based on both n & p type semiconductors are fabricated. A distinctive feature of these transistors is their long-term stability, reproducibility and low voltage < 1V operation in air. Devices exhibit forward-looking electronic performances, notably, electron and hole mobility–capacitance product of 3.5 × 10–3 μF/Vs and 23 × 10–3 μF/Vs, respectively, surpassing ones of the previously reported water-gated OFETs. Furthermore, the observed devices responsivity to humidity provides promising opportunities for sensing applications. We then demonstrate, for the first time, the implementation of honey-based integrated circuits: inverting logic gate and ring oscillator. Lastly, honey-gated OFETs are fabricated on edible flexible tattoo-paper substrate that acts as a versatile platform for organic edible electronics [1]. [1] Giorgio E. Bonacchini, Caterina Bossio, Francesco Greco, Virgilio Mattoli, Yun‐Hi Kim, Guglielmo Lanzani, and Mario Caironi, Tattoo‐Paper Transfer as a Versatile Platform for All‐Printed Organic Edible Electronics, Advanced Materials 30 (14): 1706091, 2018

Authors : Sangita Kundu, and Nilmoni Sarkar
Affiliations : Senior Research Fellow, Department of Chemistry, Indian Institute of Technology Kharagpur-721302 West Bengal India; Professor, Department of Chemistry, Indian Institute of Technology Kharagpur-721302 West Bengal India

Resume : In vivo thermometry is an emerging area of research to understand the temperature regulation within and between the organs and to describe the intricate cellular metabolic processes. Highly green luminescent gold nanoclusters (Au NCs) have been successfully implemented as potential sensors for temperature measurement in vivo because of their small size, biocompatibility, quick equilibration with temperature and most importantly their response reflect the thermal environment in the physiological temperature range. This thermoresponsive fluorescence lifetime based nanoprobe based on the supramolecular host-guest recognizition employing 6-aza-2-thiothymine and L-arginine (L-Arg) shows selective cytoplasm staining and robust response to temperature. Subsequently, fluorescence lifetime imaging microscopy (FLIM) has been employed to elucidate intracellular nanothermometry inside living cells. In depth understanding of protein-Au NCs interaction offer a promising avenue to rationally design target-specific fluorescent biolabeling agent. Because of the small surface area of the Au NCs, these probes open up the new possibility of combining therapeutic moieties having the specific cytoplasm targeting capability and simultaneously monitor the temperature assisted drug- delivery/release.

Authors : Hyoung-Joon Jin
Affiliations : Inha University

Resume : Recently, nanocellulose has gained much attention as a potential reinforcing material in the field of composites owing to its excellent mechanical, biodegradable, renewable, and biocompatible properties. Cellulose nanocrystals (CNCs), which combine natural abundance and excellent mechanical properties, are a promising candidate for use as polymer reinforcement agents. CNCs are employed in a variety of fields to enhance the mechanical properties of products such as cosmetics, pharmaceuticals, paper coatings, and additives in paper and paperboard. The high density of hydroxyl groups provides the hydrophilic properties of these materials, making them also suitable for the production of hydrogels. However, because of their hydrophilic nature, CNCs are mainly used to control the hydrophilic properties and improve the mechanical properties of hydrophobic polymers, especially synthetic polymers. On the other hand, in the case of hydrophilic polymers, an additional physicochemical cross-linking process is required because CNCs only affect the mechanical properties of composite materials. In this work, dual functionalized-CNCs as a nanofiller was used to develope a gelatin-based nanocomposite film with enhanced mechanical properties and hydrolytic stability . Through the sodium periodate oxidation reaction, C2-C3 cleavage of cellulose molecules was generated and the aldehyde groups were introduced on these carbon atoms. Using dialdehyde CNCs, interfacial covalent bonding was formed between the aldehyde group of the CNCs and the amine group of the gelatin molecule through a nucleophilic addition reaction. To investigate the effect of the dialdehyde CNC content on the properties of the biocomposite, the mechanical and physicochemical properties and the hydrolytic stability of the fabricated biocomposite films were studied using various analysis techniques. The bi-functional CNCs, which can have both cross-linking and reinforcing effects, would expand the fabrication and exploration of high-performance biopolymer nanocomposites. The gelatin-based nanocomposite film developed by this strategy has greatly improved mechanical properties and hydrolytic stability, which suggests that gelatin can be used not only as a hydrophilic polymer but also as a universal polymer.

Authors : Doohun Kim, Kyeong-Hee Lee, Bongju Kim, Minwoo Kim
Affiliations : Korea Electro-technology Research Institute (KERI), Changwon 51543, Republic of Korea; Dental Life Science Research Institute & Clinical Translational Research Center for Dental Science, Seoul National University Dental Hospital, Seoul 03080, Republic of Korea; Dentis Ltd., Daegu 41065, Republic of Korea

Resume : To increase bone regeneration around a titanium implant, nanopattern surfaces with 10 nm nanopores in 120 nm dimples were produced by electrochemical nanopattern formation (ENF). The ENF surfaces were obtained by removing the TiO2 nanotube layers prepared by an anodization process. To determine the effect of the ENF surface in vitro, cell proliferation assays, alkaline phosphatase (ALP) activity assays, Alizarin red staining, western blotting, and immunocytochemistry were performed. Atomic force microscopy and scanning electron microscopy analysis showed that the ENF surface had an ultrafine surface roughness with highly aligned nanoporous morphology. In vitro, human mesenchymal stem cells exhibited increased cell proliferation and enhanced expression of osteogenic molecules such as ALP, osteopontin, and osteocalcin on ENF surfaces compared to the TiO2 nanotube surfaces. Therefore, ENF is a promising technique for coating osteointegrative implant materials.

Authors : Siyu Xiong, Prof Andrew Davenport, Prof Kwang-Leong Choy
Affiliations : Siyu Xiong, Prof Kwang-Leong Choy: Institute for Material Discovery, University College London, London, WC1E 7JE, United Kingdom Prof Andrew Davenport: Centre for Nephrology, Royal Free Hospital, London NW3 2PF, United Kingdom

Resume : Indoxyl sulfate (IS) has been reported to not only accelerate the progression of chronic kidney disease (CKD), but also increase the risk of cardiovascular disease by increasing the oxidative stress of CKD patients. Current haemodialysis facilities are immobilized due to huge dialysis machine and dialysate bag. Some wearable artificial kidney (WAK) devices are still based on diffusion principles, which could not fundamentally solve the problem of portability. We report on a novel IS absorbent material based on biocompatible polycaprolactone-chitosan (PCL-CS) nanocomposite fibrous structure. This composite absorbent material has a high internal surface to volume ratio so as to greatly increase the interaction between the IS and chitosan, which exhibits character of high binding affinity to IS molecules. The PCL-CS absorbent material was fabricated by 2D-controlled close-range electrospinning. The surface morphology and chemical composition were characterized by a combination of scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The IS molecule absorption of the PCL-CS absorbent was quantitatively evaluated. This novel nanocomposite absorbent may provide new sights for the removal of metabolic toxins in a portable way.

Authors : Kaiwei Tang(1)*, Guomin Wang(1), Paul K. Chu(1).
Affiliations : (1) City University of Hong Kong, China

Resume : Au decorated aligned ZnO nanorods are synthesized on the Ti substrate by the silane coupling agent-assisted seed layer formation and hydrothermal process with the following magnetron sputtering of gold nanoparticles. The Au@ZnO systems inactivate about 80% of Escherichia coli in the first 1h culture in the dark through an extracellular electron transfer mechanism instead of ROS production and zinc ion releasing. Electrons on the respiratory chains of the bacterial membrane transfer to the surface of the materials, making bacteria lose electron until death. At the same time, the transferred extracellular electrons convert into the bacterial current by the electrochemical workstation, primarily demonstrating the property of the materials to function as a sensor of bacteria counting and status observation. This work sheds light on the detailed understanding of antibacterial behaviors of ZnO-based materials especially at the early stage of bacteria-material contact and provides insights for the designing of devices with both antibacterial and detection applications.

Authors : Tae Ann Kim*, Jun Beom Pyo, Sang-Soo-Lee, and Min Park
Affiliations : Photo-electronic Hybrids Research Center, Korea Institute of Science and Technology, Republic of Korea

Resume : Developing materials that can absorb large amounts of strain without fracture or significant degradation in their electronic properties is important to realize stretchable electronics. Here, stretchable conductors made from reduced graphene oxide/polydimethylsiloxane (r-GO/PDMS) composite are fabricated to achieve stable resistance changes under uniaxial stretching. By controlling the weight fraction of GO in water, highly aligned and porous GO structure is obtained over 0.5 wt% of GO solution. After thermal and chemical reduction of GO to r-GO film, PDMS is infiltrated into pores of the film to make free-standing and elastic composites. Electrical percolation of r-GO in the composite is achieved at the r-GO concentration over 0.3 wt% along with improvement of electrical conductivity. However, highly aligned r-GO/PDMS composite only exhibits notable suppression of resistance change under the large deformation of 100% strain. The resistance change of 0.3 wt% r-GO/PDMS composites at 100% strain is over 7 times higher than that of 0.7 wt% r-GO/PDMS composites, exhibiting the dramatic effect of r-GO alignments. Even after repeated stretching/releasing cycles, the variation in resistance from 0 to 70% of strain is stable. The stretchable conductor based on the aligned r-GO/PDMS composite was successfully applied as a resistive strain sensor.

Authors : N. Tsierkezos, U. Ritter, P. Scharff (2), O.Ivanyuta, E. Buzaneva (1)
Affiliations : 1National Taras Shevshenko University of Kyiv, 64/13, Vladimirska Str., 01033, Kiev, Ukraine e-mail: 2 Technische Universitat Ilmenau, Institut fur Chemistry & Biotechnoly, Postfach 100565, 986884 Ilmenau, Germany

Resume : The trend of organic thin films research toward conductivity - photovoltaic chip has allowed using the/films from ds-DNA –templated naocarbons molecular layers obtained by biotechnology. On the base of the review deals to analysis of electronic properties, photosensitivity, photoelectron moving force (PhEMF) and their stability under UV-vis irradiation for nanocarbon films with DNA molecules, we selected the thin films from silf-assembled layers of fullerene C60/C60 oxygen derivatives/ds-DNA on silicon for the detail investigations. The developing model of conductivity, and photovoltaic effect, in these layerss takes in account that C60 molecule is an acceptor of electrons. And the effect enhances with formation of C60 oxygen derivatives: 6-5 open fullerene C60 as we showed in first time [1].The evidence of self-assembling of these layers with (ds-DNA) in the nanostructured films on Si surface were obtained on the base of STM and SEM images of the films with the assembles (8-10 and 30-40 nm in diameter).The conductivity of the films was modulated by diode characteristics of fullerene C60/6-5 open fullerene C60 and C60/ ds-DNA contacts for n-type semiconductors fullerenes witch were determined by STP - tunneling spectroscopy. The discovered dynamic behavior of photosensitivity to 200-400 nm irradiation and PhMF appearance (0,25-0,37 eV) at 400-1000 nm irradiation (during 10 min - 1 h) of these films with several structures allow to consider these nanostructured layers/films as conducting/ photovoltaic chips. The examples for an application of these chips based on conductivity/photovoltaic models which have been developed for organic nanostructured thin films from C60 fullerene/ds-DNA molecular assemblies are discussed. Ref.,{1] E. Buzaneva, A. Gorchinskiy, P. Scharff, K. Risch, A. Nassiopoulou, C. Tsamis, Yu. Prilutsyy, O. Ivanuta, A. Zhugayevych, D. Kolomiyets, A. Veligura, DNA, DNA/Metal Nanoparticles, DNA/Nanocarbon and Macrocyclic Metal Complex/Fullerene Molecular Building Blocks for Nanosystems: Electronics and Sensing, In Book Frontiers of multifunctional integrated nanosystems, Eds: Eugenia Buzaneva and Peter Scharff, NATO Science Series, II-Mathematics, Physics and Chemistry–Vol 64, Kluwer Akademic Publishers, Dordrecht, 251-276, 2004.

Authors : Wiktoria Lipińska (1)*, Katarzyna Siuzdak (1), Piotr Barski (2), Agnieszka Lindstaedt (2), Katarzyna Grochowska (1)
Affiliations : (1) Centre for Plasma and Laser Engineering, The Szewalski Institute of Fluid-Flow Machinery, Fiszera 14, 80-231 Gdańsk, Poland; (2) Prochimia Surfaces, Zacisze 2, 81-823 Sopot, Poland

Resume : Nowadays, diabetes became a serious problem since everyday 400 million people face with it. Following that, extensive research works towards the enzymatic electrochemical biosensors are undertaken since those devices can selectively monitor glucose level in human body fluids. In this work, preparation method and electrochemical activity of the enzyme-functionalized Au-Ti electrode are presented. Firstly, the conductive platform was fabricated via anodization process of Ti and further chemical etching that results in regularly distributed nanodimples. Secondly, the thin Au film sputtering followed by the thermal annealing leads to the Au nanoparticles formation in the host Ti hollows revealed by scanning electron microscopy. Thirdly, the surface of obtained material was modified with glucose oxidase by the hybrid immobilization route. The first layer was the self-assembled organic monolayer composed of HSC11H22EG6OCH2COONHS chains. Then, GOx enzyme was anchored via the N-hydroxysuccinimide ligand using the cross-linking immobilization route. Basing on the cyclic voltammetry measurements performed in PBS the sensitivity of 43.926 μMcm-2mM-1 was reached within glucose concentration range of 0.006-2.345 mM while the limit of detection was as low as 5.331 μM. Additionally, the measurements in artificial sweat and saliva as well as human serum were carried out to show the performance in the real conditions. Research is financed by NCBR via LIDER/2/0003/L-8/16/NCBR/2017 grant.

Authors : Aishee Dey* (1), Preetam Guha Ray (2), Santanu Dhara (2) & Sudarsan Neogi (1)
Affiliations : (1) Department of Chemical Engineering, Indian Institute of Technology Kharagpur, India - 721302 (2) School of Medical Science and Technology, Indian Institute of Technology Kharagpur, India - 721302.

Resume : Nanosized zinc oxide is gaining importance in the biomedical field due to its commendable antibacterial efficacy. The increased specific surface area of the nanoparticles ensures enhanced particle activity, hence proving itself to be more effective than the bulk sized particles. The fluorescence property of zinc oxide arised due to defects and its use in biomedical field is recently being explored. In this work, shape controlled fluorescent zinc oxide nanoparticles was synthesized by a facile and novel wet chemical technique. Morphological and physico-chemical characterization of the nanoparticles were performed in order to affirm structural and functional aspects of the nanoparticles. Further, bactericidal activity of the 30 nm sized ZnO nanoparticles were tested against B. subtilis and E. coli. It was observed that 100 ppm conc of the nanoparticles were able to demonstrate 99% (5 log reduction) in bacterial colony size in 6 h of incubation time. Similar results were also witnessed in zone of inhibition, growth curve analysis, DNA leakage assay and live-dead assay, thus confirming bactericidal efficacy of the nanoparticles. Further, in vitro cytocompatibility was also performed with L929 fibroblast cells in order to evaluate toxicity profile of the nanomaterial. The excellent bactericidal efficacy coupled with low toxicity profile renders the fluorescently active sub-50 nm sized ZnO nanoparticles as an excellent alternative for next generation non-invasive biomedical applications. References 1. Ray, Preetam Guha, et al. "Sonication Assisted Hierarchical Decoration of Ag-NP on Zinc Oxide Nanoflower Impregnated Eggshell Membrane: Evaluation of Antibacterial Activity and in Vitro Cytocompatibility." ACS Sustainable Chemistry & Engineering 7.16 (2019): 13717-13733. 2. Alekish, Myassar, et al. "In vitro antibacterial effects of zinc oxide nanoparticles on multiple drug-resistant strains of Staphylococcus aureus and Escherichia coli: An alternative approach for antibacterial therapy of mastitis in sheep." Veterinary world 11.10 (2018): 1428.

Authors : Wenyi Hu* (1), Yuval Elani (2), and Claudia Contini (1).
Affiliations : (1) Department of Chemistry, Imperial College London, London, UK (2) Department of Chemical Engineering, Imperial College London, UK.

Resume : The current state of the art in artificial cell science relies on the construction of lipid vesicle membranes whose inherent properties, however, limit their applications particularly in sustaining cell internal biochemistry and communication between neighbors. Polymersomes are considered as ideal alternatives to liposomes because of their enhanced stability, chemical versatility, chemical and mechanical resistance. Nevertheless, the use of polymeric vesicles is restricted due to their low membrane permeability. Here, a suite of porous polymer membranes which is intrinsically permeable to molecules of defined sizes is explored as chassis for artificial cell, with the ideal functionalities of retaining specific molecules (e.g. protein and DNA) but allowing influx and efflux of small molecules (e.g. metabolites, signal molecules and mRNA). The porous vesicles are expected to form directly by self-assembly of copolymers, and the porosity is hypothesized to be controlled by varying experimental parameters during extrusion. The combinations of copolymers involve the mixtures of diblock copolymer PEG-PBD and triblock copolymer PEG-PPO-PEG with different molar ratios, and the solutions of two or more triblock copolymers PEO-PPO-PEO with different head-to-tail ratios. The porous polymer vesicle is novel in artificial cell science; thus, many potential applications can be tapped into, including for spatially-segregated biochemical reactions in polymer-based synthetic organelles.

Authors : Xiaozhen Li, Shuang Tian, Zhongming Huang, Yafang Xiao, Xiao Cui, Chun-Sing Lee
Affiliations : City University of Hong Kong

Resume : High-performance photothermal agents remain highly required for realizing efficient tumor photoelimination. However, low-photoconversion and non-biodegradability of photothermal materials seriously lead to restricted tumor suppression or unavoidable biosafety issues. In such, a major mission is focused on developing efficient and dependable agents with prominent photothermal conversion efficiency for maximized cancer photoelimination. Herein, we developed a biodegradable photothermal therapeutic (PTT) agent, π-conjugated oligomer nanoparticles (F8-PEG NPs), for highly efficient cancer theranostics. By exploiting an oligomer with excellent near-infrared (NIR) absorption, the nanoparticles show a high photothermal conversion efficiency (PCE) up to 82% surpassing those of reported inorganic and organic PTT agents. In addition, the oligomer nanoparticles show excellent photostability on one hand but also good biodegradability. The F8-PEG NPs are also demonstrated to have excellent biosafety and PTT efficacy both in vitro and in vivo. This contribution not only proposes a promising oligomer-based PTT agent, but also provides insight into developing highly efficient nanomaterials for cancer theranostics.

Authors : Xiao Cui,Yafang Xiao,Xiaozhen Li,Shuang Tian,Qi Zhao
Affiliations : Department of Chemistry, Institution Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, Address 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, P. R. China

Resume : Exploitation of new photosensitizes with sufficient oxygen-independent O2−• generation is highly desirable for overcoming hypoxia in photodynamic therapy (PDT). Herein, we report the first demonstration using a radical molecule as a new photosensitizer for hypoxic-overcoming photodynamic therapy. After self-assembling the radical molecules into nanoparticles (NPs), the NPs show good water dispersibility, good biocompatibility, board near infrared (NIR) absorption and emission at ~ 800 nm. Significantly, radical NPs remain stable in various biological solution, 100-days exposure to ambient environment, and long-term laser irradiation, which are ultrastable compared to many reported radical materials. Efficient 1O2 and O2−• generation and cytotoxicity were observed upon NIR irradiation. More importantly, even under hypoxia condition, sufficient oxygen-independent O2−• generation and cytotoxicity were observed addressing the most important hurdle for successful PDT in oxygen-deficient tumor microenvironment. This work demonstrates that stable radical molecules could have interesting properties suitable for novel biomedical applications.

Authors : Yafang XIAO, Xiao CUI, Shuang TIAN, Xiaozhen LI, Qi ZHAO.
Affiliations : Department of Chemistry Institution Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, Address 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, P. R. China

Resume : Rational manipulation of energy utilization from excited-state radiation of theranostic agents with donor-acceptor structure is relatively unexplored. Herein, we present an effective strategy to tune the exciton dynamics of radiative excited state decay for augmenting two-photon nanotheranostics. As a proof of concept, two thermally activated delayed fluorescence (TADF) molecules with different electron-donating segments are engineered, which possess donor-acceptor structures and strong emissions in the deep-red region with aggregation-induced emission characteristics. Molecular simulations demonstrate that change of the electron-donating sections could effectively regulate the singlet-triplet energy gap and oscillator strength, which promises efficient energy flow. Two-photon laser with great permeability is used to excite TADF NPs to perform as theranostic agents with singlet oxygen generation and fluorescent imaging. These unique performances enable the proposed TADF emitters to exhibit tailored balances between two-photon singlet oxygen generation and fluorescence emission. This result demonstrates that TADF emitters can be rationally designed as superior candidates for nanotheranostics agents by the custom controlling exciton dynamics.

Authors : Shi Mo (1), Paul K. Chu, *(1), Kaiwei Tang (1), Babak Mehrjou (1), Guomin Wang (1), Huaiyu Wang (2).
Affiliations : (1) Department of Physics, City University of Hong Kong, Hong Kong, China; (2) The Shenzhen Institutes of Advanced Technology, The Chinese Academy of Science (CAS), China.

Resume : Sheet-like structures such as nanoflake and nanolamellae are of great interest in biomedical engineering, because the sharp edge or large surface area can affect the bacterial and cellular behaviors on attaching to these structures. The nanolamellar arrays can be readily constructed on the surface of polyetheretherketone (PEEK) through one-step plasma treatment, showing a re-crystallization and time-dependent growth process. The gap, thickness and size of the nanolamellar array are controlled by adjusting the volume of plasma injection and the treatment time, which in turn affect the antibacterial activity and the growth of osteoblasts. The sharp edge and dimensional parameters of the nanolamellar arrays contribute to the antibacterial activity. However, due to the large size of the osteoblast and the stiffness of the cell membrane, the osteoblasts can grow on the arrays, which is affected by the dimension of the arrays. The improved osteoblast proliferation also renders it desirable osteointegration activity. The overall results in this work offer insights into the antibacterial behavior and the osteogenesis of the nanolamellar arrays, thus assuring this type of structure better application in biomedical engineering.

Authors : Ting-Yu Lu, Ching-Cheng Tsai, and Jiashing Yu*
Affiliations : Department of Chemical Engineering, National Taiwan University

Resume : Human adipose-derived stem cells (hASCs) can differentiate into multiple lineages and be harvested abundantly. However, the expression of pluripotency markers, which is important for the renewal and differentiation capabilities of hASCs, decreases during monolayer culture. Increasing evidence has proven that cells aggregated to form cell spheroids in 3D cell cultures better mimic the in vivo microenvironment and can enhance the expression of stemness markers. In this study, first, uniform hASC spheroids were formed by seeding cells in agarose microwell plates, and the size of the spheroids could be adjusted. Most importantly, the stemness expression of the spheroids increased significantly. Additionally, we utilized microbial transglutaminase (mTG), which is an enzyme that exhibits highly specific activity over a wide range of temperature and pH, to crosslink gelatin. The enzymatic crosslinking reaction is milder than physical and chemical methods, which may lead to cell death. The properties of the gelatin/mTG hydrogel were evaluated in detail. In addition, the spheroids were encapsulated in the 3D hydrogel successfully. The results showed that the hydrogel has low toxicity to the cells, which significantly proliferated in the 3D hydrogel. Moreover, the analysis of the differentiation potential indicated that the cell spheroids in the 3D hydrogel exhibited good activity, especially adipogenesis and chondrogenesis, compared to the cell suspension group. Furthermore, the in vivo data confirmed the excellent injectability and biocompatibility of the 3D hydrogel. Second, we demonstrated that cell spheroids entrapped in gelatin hydrogel can accelerate wound healing. To evaluate the in vivo effects of these spheroids/hydrogel system, we applied hASC single cells/hydrogel and hASC spheroids/hydrogel in a designed rat skin repair model. Results showed that skin wounds treated with hASC spheroids hydrogel system had faster-wound closure and a significantly higher ratio of angiogenesis. Based on the in vitro and in vivo results, the self‐assembled hASC spheroids hydrogel system may be a promising cellular source for skin tissue engineering and wound regeneration.

Authors : Seungje Lee*(1), Minji Ko(1), Soomin Ahn(1), Seo Yeon Shin(1), Gang Yeol Yoo(2), Woong Kim(2) and Young Rag Do(1)
Affiliations : (1) Department of Chemistry, KOOKMIN University, Seoul, Republic of Korea, (2) Department of Materials Science and Engineering, Korea University

Resume : Multifunctional anti-reflective (AR) SiO2 nano-rod structures were fabricated on glass on an ITO substrate using nano-spheres in a hexagonal-closed-packing array in an attempt to improve the efficiency of light extraction. Compared to the reflection of flat ITO/glass substrates, surface reflection of AR patterned ITO/glass decreases with gradual change of refractive index, resulting in an increase in the amount of transmitted light. SiO2 nano rod structures were fabricated by a combination of polystyrene (PS)-based nano-sphere lithography (NSL) and reactive ion etching (RIE). Dimensions of height and diameter can be adjusted by selective gas etching process. O2, CHF3, and Ar gas were selected to fabricate the SiO2 nano structure. Finally, moth-eye patterned AR SiO2 nano structures were fabricated. The measurement results for our nanostructure (diameter: 150 nm, height: 400 nm) showed a 2% reflectance reduction in the 380~780 nm range compared to reflectance of flat ITO/glass substrate. Due to the deposition of SiO2 layer by Plasma Enhanced Chemical Vapor Deposition (PECVD), super hydrophilic nano patterns were obtained. To obtain an anti-fogging effect, we performed self-assembled monolayers (SAMs) treatment by applying perfluoro siloxane (PFS) on AR patterned SiO2 nanostructure. A field emission scanning electron microscope (FE-SEM) was used to investigate the morphology of the AR-patterned SiO2 nanostructure. Hydrophobicity was measured by contact angle measurement.

Authors : Miryam Criado-Gonzalez1,2; Alejandro Hernandez-Sosa3; Maria Rosa Aguilar3,4; Luis Rojo3,4; Fouzia Boulmedais1; Rebeca Hernández3
Affiliations : 1. Université de Strasbourg, CNRS, Institut Charles Sadron, Strasbourg, France 2. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, Strasbourg, France 3. Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Madrid, Spain 4. Biomedical Research Networking Center in Biomaterials, Bioengineering and Nanomedicine, CIBER-BBN, Monforte de Lemos, 5. 28029 Madrid, Spain

Resume : We report on the development of interpenetrating polymer networks (IPN) for specific application in osteochondral regeneration based on the employment of Localized Enzyme Assisted Self-Assembly (LEASA) systems on hydroxyapatite nanoparticles (HA-NPs) as building blocks for the construction of 3D peptide based hydrogels and their combination with alginate hydrogels crosslinked with bioactive cations: Ca2+, Zn2+ and Sr2+. Silica nanoparticles have been recently reported to control the self-assembly of a peptide based hydrogel onto their surface by enzymatic transformation of non-interacting peptides into hydrogelators. To the best of our knowledge, this is the first time that HA nanoparticles are employed as templates for peptide gelation. The combination of alginate and a peptide network as an IPN gives rise to a bioactive material able to be printed, with modulated mechanical properties depending on the concentration of HA nanoparticles and the crosslinker: polymer ratios. As a first step, UV-vis experiments demonstrated the successful adsorption of alkaline phosphatase (ALP) onto HA-NPs. A gel was obtained after 24 hours when ALP-HA-NPs were put in contact with a solution of Fmoc-FFpY peptide. Then, the formation of the IPN was ascertained through morphological and rheological measurements. The determination of the yield stress, thixotropic properties and gelation time allowed to assess the suitability of the proposed material for bioprinting.

Authors : Dr., Docent Oleksandr .Ivanyuta
Affiliations : Taras Shevchenko National University of Kyiv,64/13, Volodymyrska Str., Kyiv, 01601, Ukraine

Resume : Excessive microwave exposure in the early 1970s led to injuries and fatal accidents. The higher the power of microwave radiation, the greater the pathological changes in nerve cells. The study aims to investigate the effect of microwave radiation on botanic and animal DNA. [1] DNA of lily flowers and cattle were selected for this study. DNA were exposed for 1 min a day 10 h for 15 days at 1 - 30 GHz frequency (power density, 0.1 µW / cm (2)). The whole-body specific absorption rate was estimated to be 5 µW / kg. Exposure took place in a ventilated resonator surface wave placed in a rectangular waveguide. After completion of the exposure period, DNA were samples conductivity was measured. Experiments were performed in a blind manner and repeated. After completion of the exposure period, DNA were samples conductivity was measured. Experiments were performed in a blind manner and repeated. A significant decrease conductivity was after 9 days of exposure for DNA lily flowers as compared with DNA cattle after 14 days exposed. The study concludes that a reduction in conductivity or an increase in sample volume may cause significant damage in brain due to chronic exposure of these radiations. I report on a new method of electrodynamic modeling of the complex biological objects. The method is based on the computer experiment realized with CAD systems. One-way analysis of variance method was adopted for statistical analysis. Simulations have with CAD systems shown that microwaves in the frequency range from 1 to 30 GHz can penetrate the cranium and that <20% of them can penetrate the deep brain, where they can penetrate <1-4 cm into the brain. These biomarkers clearly indicate possible health implications of such exposures. The great attention is given to the influence of microwave electromagnetic field on a human body, first of all, on a human head. 1. Kesari KK, Kumar S, Behari J. Pathophysiology of microwave radiation: Effect on rat brain. Appl Biochem Biotechnol. 2012;166:379–388

Authors : Susloparova, A.* (1), Mahdi, G. (1), Guérin, D. (1), Halliez, S. (2), Colin, M. (2), Buée, L. (2), Coffinier, Y. (1), Dargent, T. (1), Alibart, F.(1,3), Pecqueur S. (1).
Affiliations : (1) Univ. Lille, CNRS, Centrale Lille, Yncréa ISEN, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000 Lille, France (2) Université de Lille, Univ. Lille, Inserm, CHU-Lille, Jean-Pierre Aubert Research Centre (JPARC, UMR - S 1172), 59045 Lille, France (3) Laboratoire Nanotechnologies & Nanosystèmes (LN2), CNRS, Université de Sherbrooke, J1X0A5, Sherbrooke, Canada.

Resume : One great challenge for bio-sensing is to ensure that detection/transduction of biochemically rich systems is fully mastered to reliably record relevant information descriptors. For neuro-sensing with microelectrode arrays (MEAs), signal transduction lies at the interface between extracellular ionic currents and the electrodes. By chemically/morphologically engineering electrode materials’ interface to ensure the best electrochemical surface impedance, the aim is to find the right materials that detect ionic signals from neurons and transduce them into electronic signals with the lowest information loss. In this direction, we show how to lower the surface impedance of microelectrodes by electropolymerization of synthesized thiophene-based monomers, functionalized for higher cell biocompatibility and higher electrochemical performances. By in-situ monitoring of impedance change with voltage-ramped impedance spectroscopy, we systematically screened electrical and chemical deposition conditions to control the materials’ morphology (confirmed by atomic force and electron microscopy) reaching lower impedances than commercial MEAs and comparable to state-of-the art spin-coated polymers. With the presented preliminary biocompatibility tests, we aim to show that unconventional deposition processes can access to wider ranges of materials’ chemistry and morphology that cope with biological-constraints/electrical-performances to unlock future emerging bioelectronics technologies.

Authors : Miguel A. Jimenez-Munoz, Kenny Malpartida-Cardenas, Nicolas Moser, Pantelis Georgiou, Jesus Rodriguez-Manzano
Affiliations : Centre for Bio-Inspired Technology, Department of Electrical and Electronic Engineering, Imperial College London, London, UK

Resume : According to the WHO, Malaria is one of the most severe infectious diseases in the world, affecting almost half of the population. Thus, early diagnosis becomes critical. This disease is caused by the Plasmodium parasite whose main human-infective species are: P. Falciparum (Pf), P. Vivax (Pv), P. Ovale (Po), P. Knowlesi (Pk) and P. Malariae (Pm). Point-of-Care (PoC) systems promise to be a fast and costless alternative to current diagnosis methods, suitable for resource limited settings. Nevertheless, they still present some limitations such as low detection limit or the need of expensive optical equipment. More importantly, most of these PoC systems only focus on the detection of pan-Plasmodium, or Pf, leaving infections caused by other species undiagnosed. Increasing infections of Pv, Pk or Po in countries such as India or Malaysia have been recently reported, urging the development of new PoC devices for their detection. In previous work we have shown a novel Lab-on-a-Chip (LoC) electrochemical biosensor based on real-time LAMP and Ion-Sensitive Field Electrode transistors (ISFET) able to detect Pf. In this work, we propose to extend this system to the detection of other Plasmodium species. This methodology allow us to combine the accuracy of nucleic acid amplification with sensitive and unlabeled detection provided by the ISFET sensors. Furthermore, this LoC technology is promising for multiplexing, allowing the detection of several pathogens at once, ideal for PoC applications.

Authors : M. Chetyrkina (1), F. Talalaev (1,2), S. Kostyuk (3) and P. Troshin (1,2).
Affiliations : (1) Skolkovo Institute of Science and Technology, Russia (2) Institute for Problems of Chemical Physics of Russian Academy of Sciences (IPCP RAS), Russia (3) Research Centre for Medical Genetics (RCMG), Russia

Resume : The preventive and personalized medicine strategy for disease treatment stands apart from traditional medicine therapy. Nowadays, non-invasive monitoring and diagnostic systems based on wearable electronics and e-skin are considered as crucially important for implementation of smart healthcare technologies. However, materials for such devices should fulfill specific requirements, e.g. have a good combination of charge-transport properties, mechanical flexibility, and biocompatibility. In this work, we explored the biological effects of a series of different organic semiconductors on in vitro living system. Human embryo lung fibroblasts were incubated at standard conditions with thin films of studied materials. Cells were fixed at different time points in order to find out acute and long-term effects on fibroblasts (30 hours, 96 hours, 14 days). Flow cytometry and immunofluorescence microscopy techniques were performed with antibodies to H2A histone family member X and 8-oxoguanine for DNA damages evaluation. The occurred changes in cell metabolism were detected by real-time polymerase chain reaction, which was carried out with primers to the genes involved in apoptosis regulation and oxidative stress in cells. Results of our study demonstrated that some of the studied organic semiconductors represent biocompatible materials suitable for wearableand on-skin electronics.

Authors : Zhuyun Li, Benhui Hu, Eugene V. Makeyev, Xiaodong Chen
Affiliations : Dr. Z. Li, Dr. B. Hu, Prof. X. Chen Innovative Center for Flexible Devices (iFLEX) School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798, Singapore Prof. E. V. Makeyev Centre for Developmental Neurobiology King’s College London New Hunt’s House London SE1 1UL, UK

Resume : Cellular signalling through diffusible factors and direct cell-to-cell contacts are known to play a critical role in controlling cell viability. To understand how mechanical neuron-neuron contact affects neural survival is essential for study brain development. However, tools to systematically manipulate inter-neuron connection rarely occurs in previous studies. Herein, we developed a strategy to impose stereotypic interneuronal spacing and connectivity using topology micropatterns. With the artificial surface, we found that increased inter-neuron connectivity promotes the survival of neurons by activating neuron survival pathways. Overall, our approach helped to pave the way to improve primary neuron cell culture methodology, and more importantly, enable us to manipulate neuronal survival simply by controlling the connectivity between neurons.

Authors : Ting-Ying Wu, Zhen-Yu Guan, Chih-Yu Wu and Hsien-Yeh Chen*
Affiliations : Department of Chemical Engineering, National Taiwan University

Resume : The 3-dimensional(3D) porous materials or scaffold have been widely applied to the different fields. Here, we report an innovative manufacturing process for the fabrication of 3D porous structures containing any ingredients even the living cells via the chemical vapor deposition (CVD). This report indicates that the scaffold via sublimation/deposition process could encapsulate any substances, including living cells, growth factors and functional guiding molecules, to form a mono- or multi-functional scaffold and performed its biological function. According to the results, the survival rate of the cell-loaded scaffold via sublimation/deposition process was 80.8±5.3%. The proliferation and osteogenesis activities of MC3T3-E1 loaded in scaffold composited with ingredients, fibroblast growth factor (FGF-2) and bone morphogenetic protein (BMP-2), respectively, could be enhanced, comparing with cell-loaded scaffold without ingredients. Otherwise, osteogenesis and adipogenesis of human adipose-derived stem cells (hASCs) encapsulated in scaffold composited with PRP and cultured with induction medium performed better than the others group without PRP or induction medium. Moreover, according to the neurogenesis test, neurite length of pheochromocytoma 12 cell line (PC12) loaded in scaffold composited with ingredients, PEDOT:PSS and PRP, was 115.3 ± 26.8 μm which is the longest in different groups and tendency of neurogenesis activity were also as same as neurite length. Based on these result, the ingredients could be successfully loaded in the same Trojan scaffold, and also perform their individual functions. This novel approach and scaffold mentioned here provide another way for fabricating multifunctional scaffold with potentially broad applicability.

Authors : Dongkyu Kang * (1), (2), Jaechul Pyun (2) & Joonseok Lee (1), (3).
Affiliations : (1) Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, South Korea (2) Department of Material Science and Engineering, Yonsei University, Seodaemun-gu Seoul 120-749, South Korea (3) Division of Nano and Information Technology, KIST School, Korea University of Science and Technology (UST), Seoul * lead presenter

Resume : Upconversion nanoparticles (UCNPs) are commonly used as energy donor via a LRET (luminescence resonance energy transfer) in biological applications. In contrast to classic RET donors such as gold nanoparticles, quantum dots, UCNPs can emit near-infrared (NIR) upon the NIR excitation, which provides reduced signal-to-noise ratio due to the strong penetration and less autofluorescence in NIR region called diagnostic window. Here, we report NIR-to-NIR signal based LRET system for detection of progesterone, a natural steroid female sex hormone. We synthesized NaYF4@NaYF4:Yb,Tm@NaYF4 inert-core/active-shell/inert-shell UCNPs as LRET donor. Progesterone-HRP-IRdye and progesterone-BSA-IRdye were used as LRET acceptors with different size for efficient LRET from the UCNPs due to exact spectrum match of the UCNP’s emission and IRdye QC-1’s absorption. Anti-progesterone antibody was conjugated on the surface of water-soluble UCNPs. Progesterone was detected in NIR-to-NIR signal based LRET system via competitive immunoassay. The limitation of detection for progesterone was determined to be 1.36 pg/ml in 10-fold diluted human serum samples. We believe that the LRET system have considerable diagnostic potential, being background-free and able to rapidly detect biomarkers.

Authors : Pingqiang Cai, Zhuyun Li, Ela Sachyani Keneth, Luying Wang, Changjin Wan, Ying Jiang, Benhui Hu, Yun-Long Wu, Shutao Wang, Chwee Teck Lim, Eugene V. Makeyev, Shlomo Magdassi,* and Xiaodong Chen*
Affiliations : Prof. X. Chen, Dr. P. Cai, Dr. Z. Li, Dr. C. Wan, Dr. B. Hu, Dr. Y.-L. Wu, Y. Jiang Innovative Center for Flexible Devices (iFLEX),School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore Prof. S. Magdassi, E. S. Keneth Institute of Chemistry, Centre for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. Prof. S. Wang, Dr. L. Wang CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing, 100190, P. R. China. Prof. C. T. Lim Mechanobiology Institute, Department of Biomedical Engineering, National University of Singapore, 5A Engineering Drive 1, Singapore 117411. Prof. E. V. Makeyev Centre for Developmental Neurobiology, King's College London, New Hunt's House, London SE1 1UL, UK.

Resume : Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems led current studies to approximate it simply as an in-plane stress. To evaluate possible contribution of force vectors acting in other directions, we reconstituted double epithelium in a 3D-printed GeminiChip containing a sessile and a pendant channel. Intriguingly, the sessile epithelia were prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favored live cell delamination. Transcriptome analyses showed upregulation of RhoA, BMP2 and hypoxia signaling genes in the pendant epithelium, consistent with the onset of an epithelial-mesenchymal transition program. HepG2 microtumor spheroids also displayed differential spreading patterns in the sessile and pendant configuration. Using multilayered GeminiChip, our results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer.

Authors : Jing Yu, Chen Xiaodong
Affiliations : Nanyang Technological University

Resume : Biological processes such as morphogenesis, angiogenesis or embryogenesis involves group of cells coordinating their movements collectively to achieve specific functions, in a constricted fashion. Unlike single cell, collective cellular motions, which involve intercellular coordination, are much more complex and largely remain mysterious. Variances in environment could easily lead to changes in cell behaviors; factors such as chemical, physical or mechanical signals are common regulators in guiding cell migration. Among these factors, topological cue is a critical, yet easily neglected factor. Different from most benchside scenarios, in which migrations are allowed on infinite surfaces, the physiological microenvironment of cellular migration is usually confined. Here we built fibronectin patterns on polyacrylamide substrate to study confined cell migration. We showed that motion of cell cohort could be modeled through topological cues and confinement. Madin-Darby Canine Kidney (MDCK) cell line is used as an epithelial model to describe a form of oscillation phenomenon under various circular confinement, which amplitude and frequency vary with both the size of constrain as well as regions in the confined monolayer. Through spatio-temporal force analysis, increased intercellular tension and cell-substrate traction are observed with increasing degree of confinement. Together with confocal microscopy of actin cytoskeleton and average orientation field mapping on the cell sheet, a possible fluid-to-solid transition could attribute to this interesting collective cell motion.

Authors : Eunyoung Jeon(1)*, Joonseok Lee(1)
Affiliations : (1) Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Division of Nano and Information Technology, KIST School, University of Science and Technology (UST), Seoul, 02792, Korea

Resume : Conventional drug-eluting stents (DESs), biomedical implants, mainly use polymers or laser etching to load drugs. However, there are limitations in that polymers cause side effects such as late-thrombosis in the body and laser etching is complicated and expensive. Herein, we propose a porous nanostructured silica film for polymer-free DESs platform, which is synthesized by a sol-gel process using a catalyst, a silicon source, and structure-directing agents. As the structure-directing agents form micelles and grow on the surface, the porous nanostructured silica film is formed and has tunable pore sizes and channel lengths by adjusting reagents, molar ratios and reaction time. This suggests that a large amount of materials can be loaded, from large molecular weight molecules to small ones, and the films can be easily formed on the surface of commercial bare-metal stent. In addition, it is expected to be physically stable in the actual applications through pico-indentation experiment, expansion simulation and SEM, which evaluates the physical characteristics such as young's modulus (85.26 GPa) and hardness (2.69 GPa). Moreover, surface functionalization into hydrophobic with silane coupling agents (Octyltrimethoxysilane, Hexadecyltrimethoxysilane) allow the hydrophobic drugs to be loaded without polymers. The porous nanostructured silica film may represent an attractive platform that can be used in many other areas as well as polymer-free DESs.

Authors : Petruta Preda1,3*, Marioara Avram1,2, Diana Stan2, Bogdan Mincu2, Anton Ficai3
Affiliations : 1 National Institute for Research and Development in Microtechnologies - IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190, Bucharest, Romania 2 DDS Diagnostic SRL, 7 Vlad Judetul Street, Bucharest, Romania 3 Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 1-7 Gh. Polizu, 011061, Bucharest, Romania

Resume : Bionanocomposite films based of polycaprolactone (PCL) and fish collagen nanoparticles embedded with sea buckthorn oil were prepared by the solvent evaporation method at room temperature conditions. These materials were performed in order to be used as promising candidates in medical applications like regeneration of skin wounds and not only (surgical suture and prosthetic devices) by improvement the proliferation of fibroblast cells properties. The following raw materials were used for obtaining the polymeric composites: polycaprolactone (Sigma-Aldrich, Mw = 45,000); fish collagen extracted in the laboratory from fish waste; pure cyclohexanone (Sigma-Aldrich); acetic acid (Sigma-Aldrich) and sea buckthorn oil. Both polymeric films developed as well as collagen and sea buckthorn oil respectively have been physico-chemically and biologically characterized (FT-IR, SEM, UV-vis, EDX, HPLC, SDS–PAGE, WCA/water contact angle and fibroblast cell cultures study). Experimental results suggest that polycaprolactone films with collagen and sea buckthorn could be used for medical applications, when additional studies are required. Keywords: polymeric composites, skin wounds, fibroblast cell cultures ACKNOWLEDGEMENTS The work of PETRUTA PREDA has been funded by the Operational Programme Human Capital of the Ministry of European Funds through the Financial Agreement 51668/09.07.2019, SMIS code 124705. This work was supported by the grant COP A 1.2.3., ID: P_40_197/2016.

Authors : Jin Gu Kang, N. Ashwin K. Bharadwaj, Gaurav Chaudhary, Ashesh Ghosh, Marta C.Hatzell, Kenneth S. Schweizer, Randy H. Ewoldt, Paul V. Braun
Affiliations : Korea Institute of Science and Technology; University of Illinois at Urbana-Champaign

Resume : The study of a composite system made of semi-flexible polymeric networks combined with colloidal particles has remained in its infancy despite its ubiquitous nature in the biological space. By imparting functionalities into colloids or polymer networks, reconfigurable and active materials system that dynamically respond to external stimuli can be realized. Here, we demonstrate the 3D hydrogel composites consisting of semi-flexible fibrin networks and colloids. We develop the methods of integration of colloidal particles into fibrin networks and observe the structure of the resulting composite hydrogels. We characterize the mechanical properties by measuring oscillatory shear elastic moduli at the linear and non-linear regimes. The relation between the structure and the mechanical properties are discussed. Furthermore, we synthesize thermo-responsive hard-sphere colloids and soft-microgel colloids via atom-transfer-radical-polymerization and surfactant-free emulsion polymerization respectively. We successfully incorporate these into fibrin networks using the developed method. The changes in structure and dynamics upon the temperature change are observed and the origin of this switchable property is discussed.

Authors : A.F. Bonciu, S. Iosub, M. Filipescu, V. Dinca* and M. Dinescu
Affiliations : A.F. Bonciu 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania 3) University of Bucharest, Faculty of Physics, RO 077125, Magurele, Romania S. Iosub 2) Institute of Biochemistry of the Romanian Academy, 060031 Bucharest, Romania M. Filipescu 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania V. Dinca 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania M. Dinescu 1)National Institute for Lasers, Plasma and Radiation Physics, Magurele, Bucharest, 077125, Romania

Resume : In the last few years, Ceria (CeO2) nanostructures are thought to improve the biointerface mechanical properties, but also to stimulate the regenerating tissue biochemical activity by neutralizing the oxidative stress and stimulating cells proliferation. Nevertheless, there is a lack of information of mamallian cells on Ceria coatings, especially with well-defined nano and microstructured surface. Within this context, we present for the first time new designed pyramid shaped nanostructured ceria films obtained by Pulsed Laser Deposition (PLD), targeting the modulated response of SaOs-2 bone cells. The nanostructured surfaces obtained by PLD revealed shapes from the quasipyramidal, with rounded edges and dimensions of 90-120 nm, to the sharp edges and dimensions up to 350 nm, obtained by varying the number of pulses. The shapes and sizes of the nanostructures influenced both wettability and cellular behaviour. Nevertheless, the influence of pyramidal shaped ceria on the in vitro biological performance of SaOs-2 cells used as bone cell model was demonstrated by the differences in early adhesion and distribution of phenotype SaOs-2 cells, onto the ceria surface. In conclusion, different nanostructured ceria films obtained by PLD influenced especially the early response of osteosarcoma cell and are good candidates to improve the performance of orthopedic implants. In perspective, different cells lines response will be analysed toward establishing an osteogenic response. Acknowledgement This work was supported by the National Authority for Research and Innovation in the frame of the Nucleus Program and grants of the Romanian Ministry of Research and Innovation,CCCDI-UEFISCDI, project numbers PN-III-P1-1.2-PCCDI-2017- 0637 (MultiMonD2).

Authors : Ivanyuta O.,1 Gogotsi H.,1 Buzaneva E.,1 Ritter U.,2 Scharff P.,2
Affiliations : 1 National Taras Shevchenko University of Kyiv, Ukraine; 2 TU of Ilmenau, Institute for Chemistry and Biotechnology, Germany E-mail:

Resume : The characterization of the protein amino-acids in natural cells and mimetic medias is one of great value in the amino-acid studies. Using results of investigations for intermolecular interaction between the C60 and ds-, ss- DNA sequences [1], predictive modeling of a interface structures for the fullerol (immobilized by –OH groups fullerene C60) molecules in a cluster and this molecule at an interface with metal ion (Cu or Ag ) complex having coordinative bond to selected amino-acid (glycine, alanine, methionine, histidine) molecules [2,3] has been realized. This modeling should derive interface properties from the chemical functionalities of the constituents, while operating stimuli- response molecular nanostructures in aqueous solutions with amino-acid molecules. Modeling was carried out taking in account that these interfaces organization may increase the photoactivity and the biocompatibility to this carbon molecule counterpart. The interface designing concept as the base for the fullerol molecule in a cluster sensor and molecular ion metal complex sensor development has been discussed. Based on well established rationale of interface organization in biological macromolecules (nucleic acids, proteins), driven by non-covalent interactions (hydrogen bonding, hydrophobic effect and electrostatic interaction) in living cells, we have plan to select biomolecules for non-covalent interaction with a core of the fullerol molecule and a molecule of organic ligand in ion metal complex – amino acids. Then molecular interface organization in solutions can be revealed by photospectroscopy. From experimental results specific interface formation between [Сuх(5-amtetrazole)у]2 complex and histidine molecule for organic specially synthesis ligand with the same coordination nature of this amino-acid molecule has been determined. A special structure of a ligand and histidine molecules gives more coordination geometry to molecular complexes, determines specific interface formation in this supramolecule.Selective coordination of such supramolecule in solutions with the histidine molecules (pH 2.7 – 12.9) was confirmed by absorbance spectra with the intensive band (600 800 nm) and it is assigned to the d – d transition of Cu. Maximum position shifted from 753 to 615 nm after adding the histidine was demonstrated. The histidine concentration effect for the solutions with the fullerol molecular clusters and the functionalized by Cu –azole ligand complex fullerol molecules was also determined. The next step is the development of controlled by light excitation interface between the fullerol, selected ion metal complex and many protein-acid molecules organization for their photo-spectroscopy recognition and single molecular biosensor controlled design. References: 1. O. Kysil, I.Sporysh, E.Buzaneva, T. Erb, G.Gobsch, U.Ritter, P.Scharff, Mat. Sc. and Eng. B169/1-3 (2010) 85. 2. O. A. Bondar, L. V. Lukashuk, A. B. Lysenko, H. Krautscheid, E. B. Rusanov, A. N. Chernega, K V. Domasevitch, Cryst. Eng. Comm, 10 (2008), 1216. 3. J. G. Mesu; T. Visser; F. Soulimani; E. E. van Faassen; P. de Peinder; A. M. Beale; B. M. Weckhuysen, Inorg. Chem., 45/5 (2006) 1960.

Authors : Gryn D.V., Naumenko A.P., Gubanov V.O.
Affiliations : Taras Shevchenko National University of Kyiv

Resume : Cisplatin (cis-Pt) is a complex of platinum which widely used as an anticancer drug. Transplatin (trans-Pt) has the same chemical formula, but different positions of atoms. Cisplatin connects to DNA in cancer cells and stops DNA replication, namely making cross-links between guanine bases. Transplatin is less reactive to DNA but also demonstrate cytotoxicity effect. In this work we make an attempt to connect the differences in the biological activity of mentioned structures with the data obtained by optical spectroscopy methods. The UV-vis spectra as well as fluorescence and phosphorescence spectra of water solutions of cis-Pt and trans-Pt were investigated at temperatures 300 and 77 K. Quantum chemical calculations were used for correct interpretation of obtained results. We also characterized these compounds using such powerful method as Raman spectroscopy.

Authors : Alkmini D. Negka, Panagiota Koralli, Maria Goulielmaki, Lida Evmorfia Vagiaki, Aristea Pavlou, Giannis N. Antoniou, Panagiotis E. Keivanidis, Dimitris Moschovas, Apostolos Avgeropoulos, Aristotelis Xenakis, Vassilis Zoumpourlis, Antonia Dimitrakopoulou-Strauss, Vasilis G. Gregoriou, Christos L. Chochos
Affiliations : Clinical Cooperation Unit Nuclear Medicine, German Cancer Research Center, 69120 Heidelberg, Germany;Institute of Chemical Biology, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, Athens, 11635, Greece; Department of Mechanical Engineering and Materials Science and Engineering, Cyprus University of Technology, Limassol 3041, Cyprus;Department of Materials Science Engineering, University of Ioannina, Ioannina 45110, Greece;National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, Athens, 11635, Greece

Resume : To date, the therapeutic properties of π-conjugated polymers especially in the form of aqueous nanoparticles, the so-called conjugated polymer nanoparticles (CPNs), have been verified merely upon laser irradiation via the well-established photodynamic and photothermal therapies. In this contribution, it is manifested for the first time the natural anticancer properties of single CPNs. This has been accomplished by studying the cell viability of new CPNs in physiological human umbilical cord mesenchymal stem cells (WJ-MSC), human colorectal carcinoma cell line (HCT116) and metastatic human melanoma cell line (WM164). The discovery of natural anticancer properties in conjugated polymers can form a new frontier in nano-biotechnology and nanomedicine and represent a new direction that is orthogonal to the more established areas of conjugated polymers for optoelectronic devices and conjugated polyelectrolyte biosensors. The combined natural anticancer and bioimaging properties of CPNs point to a new strategy for obtaining novel organic nanomaterials for cancer theranostics.

Authors : Panagiota Koralli, Alkmini Negka, Lida Evmorfia Vagiaki, Antonia Dimitrakopoulou-Strauss, Vasilis G. Gregoriou, Christos L. Chochos
Affiliations : National Hellenic Research Foundation, Institute of Chemical Biology, Athens, Greece; German Cancer Research Center (DKFZ), Clinical Cooperation Unit Nuclear Medicin, Heidelberg, Germany; National Hellenic Research Foundation, Athens, Greece

Resume : Low-bandgap aqueous conjugated polymer nanoparticles (CPNs) have been prepared and applied in biological applications, especially in bio-imaging, due to their unique optoelectronic properties.In this study, we successfully synthesized a series of conjugated polymers combining thiophene as the electron donating and quinoxaline as the electron deficient (TQs) with varying the number of fluoro atoms in the repeat unit. Water-soluble nanoparticles formatted by two different methods (nanoprecipitation and encapsulation), supporting their potential use as low-bandgap fluorescent probes for bio-imaging.The DLS measurements indicate that all CPNs in aqueous suspension have a unimodal size distribution less than 80nm.TEM & SEM measurements showed that the CPNs have spherical shape.The optical properties of the CPNs show light absorption and fluorescence emission in the UV-Vis region,tunable depending on the structure, the position and the number of fluoro atoms on the polymer backbone.It is revealed a significant reduction of the fluorescence quantum yield in aqueous media derived from severe aggregation and charge transfer induced fluorescence quenching, which can be controlled with proper rational design on the polymer backbone. Moreover, the on-going tests for cellular cytotoxicity and the ability of the obtained probes to image cancer cells are of great importance.Overall, CP dots introduce a new generation of fluorescent probes that appear to be the quintessential contrast agents

Authors : H.Gogotsi(a),,O.Ivanyuta(a),,E.Buzaneva(a),U.Ritter(b),P.Scharff(b)
Affiliations : (a)National Taras Shevchenko University of Kyiv,, 64, Volodymirska Str., 01601 Kyiv, Ukraine, (b)TU Ilmenau, Institut für Chemie and Biotechnology, Postfach 100565, 98684 Ilmenau, Germany.

Resume : The design and optical characterization of the protein amino - acids is key for understanding of the amino-acids molecules behaviour as basis sequences of biological macromolecules ( proteins) and their using as photoactive molecules, for example, in an advanced photovoltaic systems for an energy sources [1]. Therefore, we have start to design amino-acids molecules and study classic models for light excited electron transition through LUMO-HOMO band gap for the molecules and discuss ones using theories for LUMO-HOMO band gap formation for these molecules having different confirmations [2]. Then we selected amino-acid molecule for studies by the PL spectroscopy take in account that can use the laser with 408 nm wavelength (3.3 eV) for experiments. The cysteine PL excited by the laser with wavelength 408 nm( 3.3 eV) is characterized by the band in 1.5- 2.7 eV range. This band included of subbands, which are centered at 2.04, 2.24 and 2.4 eV. The present of subbands are explained by the dependence on the conformation of the molecule geometry in the cysteine layer of the HOMO-LUMO value as it is found due to compare of these subbands with calculations of electronic excitations of the cysteine conformers [2]. Therefore it is possible to use light excited electron transitions in the cysteine layer with different molecule geometry in photonic systems. 1. Koo, Suk Tai Chang, Joseph M. Slocik, Rajesh R. Naik and Orlin D. Velev, Aqueous soft matter based photovoltaic devices, J. Mater. Chem., 2011, Advanced Article.;2.R. Maul, M. Preuss, F. Ortmann, K. Hannewald, and F. Bechstedt, Electronic Excitations of Glycine, Alanine, and Cysteine Conformers from First-Principles Calculations, J. Phys. Chem. A 2007, 111, p.4370-4377.

Authors : Krasimir Koev*, Nikolaj Donkov, Hristo Naidenski, Vesslin Kussovski, Timerfayaz Nurgaliev, Latchezar Avramov
Affiliations : Assoc. prof. M.D. PhD Kr. Koev* Department of ophthalmology, Medical University 8 Byalo More str., Sofia, Bulgaria, E-mail: Assoc. prof. M.D. PhD Kr. Koev, Assoc. Prof. Dr. N. Donkov, Prof. D.Sc. T. Nurgaliev, Prof. D.Sc. LatchezarAvramov Institute of Electronics “Acad. Emil Djakov”, Bulgarian Academy of Sciences 72 Tsarigradsko chaussee blvd., 1784 Sofia, Bulgaria Prof. DVM, D.Sc. Hristo Najdenski, Assoc. prof. M.D. PhD Vesslin Kussovski The Stefan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 GeorgiBonchev str., 1113 Sofia, Bulgaria

Resume : Long-term use of ocular prostheses causes frequent ocular infections with a number of adverse effects.The antibacterial and antifungal activity was investigated of Ag-doped Al2O3 nanolayers deposited by radioactive magnetron sputtering on ocular prostheses after a two-year period. A microbiological study was performed to determine the antibacterial action of the nanocomposite Ag/Al2O3 nanolayers against gram positive and gram negative bacteria, and an antifungal action against Candida albicans. From the conducted microbiological studies we have found the preservation of the antibacterial and antifungal action of the Ag/Al2O3 nanocoating of the eye prostheses after two years. The results of microbiological studies show that antibacterial and antifungal activity has the same full inactivation by intensity for the reference period. Experimental studies show a very promising application of such antibacterial and antifungal Ag/Al2O3 nanocoating to reduce ocular infections in the placement of ocular prostheses over a prolonged period of use. Keywords: Ag/Al2O3 nanolayers, antibacterial and antifungal action, ocular prostheses coating, two-year period

Authors : Ahmaduddin Khan, Niroj Kumar Sahu
Affiliations : Centre for Nanotechnology Research

Resume : Nanoparticles mediated drug delivery system has immense therapeutic applications especially in the field of cancer treatment. The chemo-therapeutic efficacy is enhanced when combined with radiation therapy or thermal therapy (Hyperthermia). For thermalchemotherapy, appropriate functionalised magnetic nanoparticles with desired magnetic parameter are pivotal. In this direction, we synthesised a highly aqueous dispersible PEG-dicarboxylic acid functionalised iron oxide (Fe3O4) nanocluster with superior magnetic properties by a simple solvothermal approach. The well distributed nanocluster (size~200nm) are formed by the assembly of smaller nanoparticles. Fe3O4 crystallizes in inverse spinel structure with crystallite size of 16.9 nm and possess mesoporous structure with high specific surface area of 71.3 m²/g. The carboxyl group was activated using hydrazine hydrate to form pH sensitive hydrazone bond which further was utilised to attach with doxorubicin (DOX) through C=O linkage. High drug loading efficacy was achieved due mesoporous nature of the nanoparticles and carboxyl group activation. This system shows higher and sustained release at pH 4.3 in comparison to normal physiological pH of 7.4 because of labile hydrazone bond. The synthesised Fe3O4 nanoclusters are biocompatible upto a concentration of 1mg as observed in vitro in MCF-7 breast cancer cell line. Due to higher saturation magnetisation of the nanocluster, a hyperthermic temperature of ~ 43 ºC was achieved in just 105 s at nanoparticle concentration of 2 mg/ml and at the applied AC magnetic field strength of 35.2 kA/m and frequency of 316 kHz. Hence, the PEGylated mesoporous Fe3O4 nanoclusters can be effectively utilised for thermochemotherapy of cancer.

Authors : Jooran Kim
Affiliations : Korea Institute of Industrial Technology

Resume : 3D bioprinting is a widely used technology to dispense cell-laden biomaterials for rapid fabrication of complex 3D tissue constructs or artificial organs. To date, many studies have been investigated the deposition and patterning of cell-laden bioinks with a 3D bioprinter. However, the precise positioning, reasonable mechanical properties, and controlled cell distributions of constructs in 3D bioprinting system still remain technical challenges. In this study, we developed heterogeneous cell-laden microchannel network using human umbilical vein endothelial cell-cardiomyocytes by a direct patterning with a 3D bioprinter. We fabricated 3D tissue constructs consisting of the core (human umbilical vein endothelial cell -laden collagen) and the sheath (cardiomyocytes-laden gelatin methacrylate). We could achieve a stable 3D multilayered core-sheath structure with the reasonable elastic modulus. This system also facilitated cell alignment and migration within each constructs and promoted vascular network with high cell viability. Calcium imaging was used to optically probe intracellular calcium ion signals during excitation-contraction coupling in cardiomyocytes within 3D vascular network. This paper presents the new approach for fabricating vascularized heterogeneous 3D scaffolds and highly controlled deposition technique of bioinks. The cell-laden 3D constructs could be extended to serve as in vitro models for clinical cardiovascular disease researches and cardiovascular tissue regenerations.

Authors : A.I. Visan1*, I. Jinga1, I. Ungureanu1, F. Antohe2, M.C. Chifiriuc3, R. Cristescu1
Affiliations : 1 Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, Magurele, Ilfov, Romania; 2 Proteomics Department, Institute of Cellular Biology and Pathology “N. Simionescu” Romanian Academy, Bucharest, Romania; 3 Department of Microbiology, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania *Corresponding author:

Resume : Polymer-coated stents with antiproliferative drugs and growth factor have been proposed to defeat adverse reactions which occur in cardiovascular treatment. We report on successful deposition of functionalized thin films of (everolimus and paclitaxel) drugs encapsulated in complex matrices [either blends of poly(L-lactide) and collagen or nanoparticles of poly(lactic-co-glycolic acid) - polyvinyl alcohol] containing vascular endothelial growth factor (VEGF) by Matrix Assisted Pulsed Laser Evaporation (MAPLE). The morphology, hydrophilicity, and biodegradability of the composite coatings have been investigated. The main recipes of the drug functionalized polymer matrices, synthesized by MAPLE, have been validated by biological investigations, drug release profiles, and physical-chemical investigations. In vitro evaluation tests performed on the fabricated thin films have revealed great biocompatibility, that may endorse them as competitive candidates for the development of improved non-toxic surfaces resistant to microbial colonization. The proposed functionalized thin films apart from preventing the restenosis (due to selected drug inhibitors), also could eliminate the risk of late thrombosis (as the covered stent is replaced by connective tissue thanks to VEGF addition) and are expected to act as improved/appropriate/effective coatings for the next-generation drug-eluting stents.

Authors : Anita Visan1, Carmen Ristoscu1, Gianina Popescu-Pelin1, Mihai Soprony1, R. Cristescu1, Carmen Mariana Chifiriuc3, David Grossin4, Fabien Brouillet4, Ion N. Mihailescu1
Affiliations : 1 National Institute for Lasers, Plasma and Radiation Physics, Magurele, Ilfov, Romania ; 2 National Institute of Materials Physics, Magurele, Ilfov, Romania;3Department of Microbiology, Faculty of Biology, University of Bucharest, 060101; Research Institute of the University of Bucharest –ICUB, Spl. Independentei 91-95, Bucharest, Romania;4 CIRIMAT – Carnot Institute, University of Toulouse, ENSIACET; 4 Allée Emile Monso, 31030 Toulouse Cedex 4, France

Resume : We report on the successful deposition of antimicrobial chitosan-biomimetic nanocrystalline apatite –tetracycline thin films by Matrix Assisted Pulsed Laser Evaporation (MAPLE) using a KrF* excimer laser source (λ = 248 nm, ζFWHM ≤ 25 ns). Typical FTIR spectra of the obtained thin films were found to be highly similar to the spectrum of the initial powders. Scanning electron microscopy have evidenced a typical morphology characteristic to the deposition technique, advantageous for medical application, the nanoscale roughness increasing with the chitosan concentration. We have evaluated the antibacterial properties of the thin films containing chitosan and tetracycline deposited by MAPLE on titanium samples, using as model organisms both the Gram-negative E. coli and Gram-positive E. faecalis. The biocompatibility of the obtained films deposited on Ti substrates was evaluated by in vitro tests on human bone osteosarcoma cells. These tests have revealed the morphology and cellular cycle of the cells growing on the obtained thin films. The results have demonstrated that the chitosan- biomimetic nanocrystalline apatite-tetracycline composite thin films have improved the bone formation and further facilitated the anchorage between the bone and prosthesis, thus validating the MAPLE efficiency.

Authors : Iole Venditti 1, Chiara Battocchio 1, Luca Tortora 1, Giovanna Iucci 1, Martina Marsotto 1, Marina Porchia 2, Francesco Tisato 2, Maura Pellei 3, Carlo Santini 3 Corresponding Author, e-mail:
Affiliations : 1 Sciences Dept. Roma Tre University of Rome, Italy 2 ICMATE, National Research Council (CNR), Padua, Italy 3 School of Science and Technology, Chemistry Division, University of Camerino, Italy

Resume : Gold nanorods (AuNRs) are successfully employed in drug delivery, biosensors, and biotechnologies [1,2]. Their wide success is due to their unique chemical properties, biocompatibility, easy, cheap and versatile synthesis. In this framework, hydropilic AuNRs were synthetized with the aim to obtain strongly hydrophilic nanomaterials, suitable for drug delivery. AuNRs were investigated by UV-Vis-NIR, FT-IR and HR-XPS spectrocopies, confirming nanosize, with typical surface plasmon resonance (SPR) bands at 550 nm and 900 nm, and surface functionalization by ascorbic acid (AA) and cetyl trimethyl ammonium bromide (CTAB). Therefore, AuNRs were used as drug delivery system for copper (I)-based anti-tumor complex namely [Cu(PTA)4]+[BF4]- (PTA = 1,3,5-triaza-7-phosphadamantane) [3-5]. The loading procedures and efficiency of Cu(I) complexes on the AuNRs surface were optimized in order to control their bioavailability and release. References [1] Park K., Drummy L. F., Wadams R. C., Koerner H., Nepal D., Fabris L., Vaia R. A. Growth Mechanism of Gold Nanorods. Chem. Mater. 2013; 25: 555-563 [2] Venditti I. Engineered gold-based nanomaterials: morphologies and functionalities in biomedical applications. A mini review. Bioengineering. 2019; 6(2): 53 [3] Gandin V., Tisato F., Dolmella A., Pellei M., Santini C., Giorgetti M., Marzano C., Porchia M. In Vitro and in Vivo Anticancer Activity of Copper(I) Complexes with Homoscorpionate Tridentate Tris(pyrazolyl)borate and Auxiliary Monodentate Phosphine Ligands. J. Med. Chem. 2014, 57 (11): 4745-4760. [4] M. Porchia, F. Benetollo, F. Refosco, F. Tisato, C. Marzano, V. Gandin. Synthesis and structural characterization of copper(I) complexes bearing N-methyl-1,3,5-triaza-7-phosphaadamantane (mPTA): Cytotoxic activity evaluation of a series of water soluble Cu(I) derivatives containing PTA, PTAH and mPTA ligands. J. Inorg. Biochem. 2009; 103 (12): 1644 [5] Fratoddi I., Venditti I., Battocchio C., Carlini L., Porchia M., Tisato F., Bondino F., Magnano E., Pellei M., Santini C. Highly hydrophilic gold nanoparticles as carrier for anticancer copper(I) complexes: loading and release studies for biomedical applications. Nanomaterials. 2019; 9: 772 Acknowledgements: The Grant of Excellence Departments, MIUR (ARTICOLO 1, COMMI 314 – 337 LEGGE 232/2016), is gratefully acknowledged by authors of Roma Tre University.

Authors : Iole Venditti1, Irene Schiesaro1, Martina Marsotto1, Maura Pellei2, Carlo Santini2, Luca Bagnarelli2, Giovanna Iucci1, Carlo Meneghini1, Chiara Battocchio1
Affiliations : 1 Dept. of Science, University of Roma Tre, Viale G. Marconi 446, Rome, Italy. 2 School of Science and Technology, Chemistry Division, University of Camerino, Camerino (MC), Italy.

Resume : Copper complexes are coming out as metal-based drugs candidates for the treatment of cancer, due to their wide structural variability, biologically accessible redox properties and bioavailability. Recently, in our quest to find suitable ligands in the design of copper-based anticancer agents, we focused our attention on the synthesis of copper complexes of bis(azol-1-yl)carboxylate ligands functionalized with biomolecules. In addition, Cu(II) complexes of alkyl bis(pyrazol-1-yl)acetate ligands have been investigated for the development of a new and more efficient promoter for the Kharasch-Sosnovsky reaction to oxidize alkenes in allyl position. Since such coordination compounds have low solubility in aqueous medium, it is necessary to design a strategic approach allowing for drug delivery. By conjugating the copper complexes with hydrophilic gold nanoparticles, it is possible to improve their solubility and stability in water, increasing their bioavailability. Moreover, these drug delivery systems allow the investigation of a slow and controlled release of copper complexes [1]. In this context, we carried out a spectroscopic investigation of the molecular, electronic structure and coordination geometry of a selection of Cu(II)-coordination compounds, by means of complementary X-ray techniques induced by Synchrotron Radiation: the molecular and electronic structure were probed by means of SR-XPS and NEXAFS, the oxidation state and the local coordination chemistry of the metal ion were assessed by Cu K-edge XAFS analysed in the near edge (XANES) and extended (EXAFS) regions.

Authors : Su-Geun Yang
Affiliations : Department of Biomedical Science, College of Medicine, Inha University

Resume : In this study, poly-L-dopa nanoparticle-based drug delivery system was designed for near-infrared light controlled trimodal therapy in which photodynamic therapy (PDT), photothermal therapy (PTT) and chemotherapy are combined to produce synergistic therapeutic effects. Poly-L-dopa nanoparticles (PLD NPs) were synthesized by oxidation and self-polymerization of L-DOPA with KMnO_4. For designing purposes of PTT, PLD NPs were modified with the photosensitizer, pheophorbide a (PheoA), via reducible disulfide linker for glutathione-responsive intracellular release. Due to the ability to convert NIR light into heat, PLD NPs could be used as a photothermal agent without further modification for PTT. Also, PLD NPs have a fluorescence resonance energy transfer- (FRET) based self-quenching effect which reduces the side effects by keeping the system photo-inactive during blood circulation. PheoA modified PLD NPs (PLD-PheoA NPs) were loaded with chemotherapy drug, doxorubicin (Dox), through ᴨ-ᴨ stacking and hydrogen bonding. The in vitro antitumor effect of PLD-PheoA/Dox NPs with trimodal PDT/PTT/chemotherapy was investigated in terms of photothermal efficiency, photoactivity, stimuli-responsive drug release, cellular uptake, and therapeutic efficacy. The best of our knowledge, this is the only study presented in literature, using poly-L-dopa nanoparticles with trimodal synergistic therapy approach for cancer treatment.

Authors : Ji-Eun Lee, Seung-Min Lee, and Kwang-Ho Lee
Affiliations : Advanced Materials Science and Engineering, College of Engineering, Kangwon National University, Republic of Korea

Resume : To make micropattern including bio-substances on nano-mesh, we developed the photo-crosslinking process using hydrogel. For in-situ micropatterning, the synthesized hyaluronic acid was empolyed with transparent micromold and UV irradiation. The photosensitive hyaluronic acid was rapidly cross-linked through the porous nano-mesh. When hyaluronic acid is patterned on the nanomesh, drugs and cells can be stably selected, and the release of drugs and the behavior of various cells can be controlled according to the patterning conditions. The immobilized material was released by diffusion, which was very precisely controlled through the coating of hyaluronic acid and nanomesh, and the theoretical verification with finite element analysis was performed. The proposed research could be used in the field of tissue engineering for local drug delivery or regeneration of damaged tissues in the body.

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08:45 Plenary Lecture - Prof. André Geim Nobel Laureate in Physics (2010) University of Manchester, U.K.    
18:30 Graduate Student Awards Ceremony    
19:55 SOCIAL EVENT    
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08:45 Plenary Lecture - Prof. Ulrike Diebold TU Vienna, Austria    
18:30 Concluding Remark: Symposium R Organizer – Prof. Peter SCHARFF    

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Symposium organizers
Emmanuel STRATAKISInstitute of Electrnic Structure and Laser (IESL)

Foundation of Research and Technology Hellas (FORTH) and University of Crete, Nikolau Plastira 1000, Voutes, Heraklion, Crete

+30 2810 3912 74
Eugenia BUZANEVATaras Shevchenko National University of Kyiv

NASU “Physical and Chemical Material Science Centre”, Volodymyrs'ka Str. 64/13, 01601 Kyiv, Ukraine

+38 044 294 26 22
Insung S. CHOI (Main)The Center for Cell-Encapsulation Research, KAIST

Dep. of Chemistry and Dep. Bio and Brain Engineering - 281, Daejeon 34141, Korea

+82 42 350 2880
Peter SCHARFFTechnical University of llmenau

Institute of Chemistry and Biotechnology, Weimarer Strasse 25, 98693 Ilmenau, Germany

+49 36 77 69 3603(04)
Thomas J. WEBSTER Northeastern University

Department of Chemical Engineering - Center Advanced Materials Research - 313 Snell Engineering Center - Boston, IMA 02115, USA

+1 617 373 6585